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  • 78
    Agilent technologies g2565aa g2565ab scanner
    G2565aa G2565ab Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies g2565aa g2565ba microarray scanner system
    G2565aa G2565ba Microarray Scanner System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies g2565aa microarray scanner
    G2565aa Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 237 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies g2565ba scanner
    G2565ba Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 156 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies g2565ca scanner
    Microarray of Slit2 produced log ratios, expression and classification of genes. ( A ) Cartoon of a chicken embryo electroporation. In green we show the putative plasmid solution with fast green being injected inside the neural tube. And the ± shows the position of the electrodes when giving them the electric pulses necessary for the plasmid to enter cells in the NT. ( B ) Workflow and sample preparation and array processing. For the microarray we processed 6 Total RNA samples (1 Control, 2 experimental in duplicates) for gene expression. RNA quality and concentration was determined on the Agilent Bioanalyzer and NanoDrop spectrophotometer. One-color labeling reactions were prepared using the Agilent Two-Color Protocol (Version 6.6) with 400 ng total RNA input. ( C ) Log ratio values collected after microarray show significantly upregulated genes (red), significantly down regulated genes (green), and not differentially expressed (yellow). Control samples were labeled with Cy5 and Experimental sample labeled with Cy3. RNA Spike A mix was used for Cy3 (experimental) samples and RNA spike B mix was used for all Cy5 (Control) samples and scanned at 3 μM resolution on an Agilent <t>G2565CA</t> High Resolution Scanner. ( D ) Percentage of gene expression upon Slit2 gain of function in microarray. The bar graphed represents 37.3% down-regulation genes and 62.7% of incidents generated up-regulated genes of NCCs Slit2 GOF vs control.
    G2565ca Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Agilent technologies microarray scanner g2565ba
    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the <t>microarray</t> data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.
    Microarray Scanner G2565ba, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 1528 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Agilent technologies g2565ca microarray scanner
    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the <t>microarray</t> data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.
    G2565ca Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 735 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies g2565aa scanner
    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the <t>microarray</t> data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.
    G2565aa Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 78/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies g2565ca scanner system
    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the <t>microarray</t> data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.
    G2565ca Scanner System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies g2565ba array scanner
    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the <t>microarray</t> data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.
    G2565ba Array Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 78/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies agilent g2566aa scanner
    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the <t>microarray</t> data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.
    Agilent G2566aa Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 80/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies g2565aa dna microarray scanner
    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the <t>microarray</t> data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.
    G2565aa Dna Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 82/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies g2565ba microarray scanner system
    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the <t>microarray</t> data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.
    G2565ba Microarray Scanner System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies g2565aa array scanner
    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the <t>microarray</t> data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.
    G2565aa Array Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 82/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies g2567aa microarray scanner system
    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the <t>microarray</t> data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.
    G2567aa Microarray Scanner System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies laser scanner g2565ca
    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the <t>microarray</t> data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.
    Laser Scanner G2565ca, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 76/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies g2565aa fluorescence scanner
    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the <t>microarray</t> data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.
    G2565aa Fluorescence Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    Agilent technologies g2565ca dna scanner
    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the <t>microarray</t> data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.
    G2565ca Dna Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 82/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies g2565ba dna scanner
    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the <t>microarray</t> data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.
    G2565ba Dna Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies microarray g2565ba fluorescent scanner
    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the <t>microarray</t> data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.
    Microarray G2565ba Fluorescent Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies dna microarray scanner model g2565aa
    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the <t>microarray</t> data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.
    Dna Microarray Scanner Model G2565aa, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies dual laser microarray scanner g2565aa
    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the <t>microarray</t> data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.
    Dual Laser Microarray Scanner G2565aa, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 81/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies dna microarray scanner g2565ca
    Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of <t>microarray</t> probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic <t>DNA</t> extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.
    Dna Microarray Scanner G2565ca, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 95/100, based on 236 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies data analysis a g2565ba scanner
    Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of <t>microarray</t> probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic <t>DNA</t> extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.
    Data Analysis A G2565ba Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies g2565aa microarray scanner system
    Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of <t>microarray</t> probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic <t>DNA</t> extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.
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    Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of <t>microarray</t> probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic <t>DNA</t> extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.
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    Gene expression profiling of marker genes in differentiated and undifferentiated MCT samples. Heat map and hierarchical clustering of all samples analyzed by <t>DNA</t> <t>microarray</t> using probes referable to the transcripts identified by class prediction analysis. Red indicates up-regulated and green down-regulated genes relative to the mean expression in all samples. For display purposes, samples in each class (differentiated and undifferentiated) were clustered separately and arranged from differentiated (left, yellow) to undifferentiated (right, red). Genes were hierarchically clustered separately based on the Pearson correlation coefficients and average linkage clustering. Units of the bar legend: absolute values.
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    Gene expression profiling of marker genes in differentiated and undifferentiated MCT samples. Heat map and hierarchical clustering of all samples analyzed by <t>DNA</t> <t>microarray</t> using probes referable to the transcripts identified by class prediction analysis. Red indicates up-regulated and green down-regulated genes relative to the mean expression in all samples. For display purposes, samples in each class (differentiated and undifferentiated) were clustered separately and arranged from differentiated (left, yellow) to undifferentiated (right, red). Genes were hierarchically clustered separately based on the Pearson correlation coefficients and average linkage clustering. Units of the bar legend: absolute values.
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    Gene expression profiling of marker genes in differentiated and undifferentiated MCT samples. Heat map and hierarchical clustering of all samples analyzed by <t>DNA</t> <t>microarray</t> using probes referable to the transcripts identified by class prediction analysis. Red indicates up-regulated and green down-regulated genes relative to the mean expression in all samples. For display purposes, samples in each class (differentiated and undifferentiated) were clustered separately and arranged from differentiated (left, yellow) to undifferentiated (right, red). Genes were hierarchically clustered separately based on the Pearson correlation coefficients and average linkage clustering. Units of the bar legend: absolute values.
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    Gene expression profiling of marker genes in differentiated and undifferentiated MCT samples. Heat map and hierarchical clustering of all samples analyzed by <t>DNA</t> <t>microarray</t> using probes referable to the transcripts identified by class prediction analysis. Red indicates up-regulated and green down-regulated genes relative to the mean expression in all samples. For display purposes, samples in each class (differentiated and undifferentiated) were clustered separately and arranged from differentiated (left, yellow) to undifferentiated (right, red). Genes were hierarchically clustered separately based on the Pearson correlation coefficients and average linkage clustering. Units of the bar legend: absolute values.
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    Gene expression profiling of marker genes in differentiated and undifferentiated MCT samples. Heat map and hierarchical clustering of all samples analyzed by <t>DNA</t> <t>microarray</t> using probes referable to the transcripts identified by class prediction analysis. Red indicates up-regulated and green down-regulated genes relative to the mean expression in all samples. For display purposes, samples in each class (differentiated and undifferentiated) were clustered separately and arranged from differentiated (left, yellow) to undifferentiated (right, red). Genes were hierarchically clustered separately based on the Pearson correlation coefficients and average linkage clustering. Units of the bar legend: absolute values.
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    Gene expression profiling of marker genes in differentiated and undifferentiated MCT samples. Heat map and hierarchical clustering of all samples analyzed by <t>DNA</t> <t>microarray</t> using probes referable to the transcripts identified by class prediction analysis. Red indicates up-regulated and green down-regulated genes relative to the mean expression in all samples. For display purposes, samples in each class (differentiated and undifferentiated) were clustered separately and arranged from differentiated (left, yellow) to undifferentiated (right, red). Genes were hierarchically clustered separately based on the Pearson correlation coefficients and average linkage clustering. Units of the bar legend: absolute values.
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    Image Search Results


    Microarray of Slit2 produced log ratios, expression and classification of genes. ( A ) Cartoon of a chicken embryo electroporation. In green we show the putative plasmid solution with fast green being injected inside the neural tube. And the ± shows the position of the electrodes when giving them the electric pulses necessary for the plasmid to enter cells in the NT. ( B ) Workflow and sample preparation and array processing. For the microarray we processed 6 Total RNA samples (1 Control, 2 experimental in duplicates) for gene expression. RNA quality and concentration was determined on the Agilent Bioanalyzer and NanoDrop spectrophotometer. One-color labeling reactions were prepared using the Agilent Two-Color Protocol (Version 6.6) with 400 ng total RNA input. ( C ) Log ratio values collected after microarray show significantly upregulated genes (red), significantly down regulated genes (green), and not differentially expressed (yellow). Control samples were labeled with Cy5 and Experimental sample labeled with Cy3. RNA Spike A mix was used for Cy3 (experimental) samples and RNA spike B mix was used for all Cy5 (Control) samples and scanned at 3 μM resolution on an Agilent G2565CA High Resolution Scanner. ( D ) Percentage of gene expression upon Slit2 gain of function in microarray. The bar graphed represents 37.3% down-regulation genes and 62.7% of incidents generated up-regulated genes of NCCs Slit2 GOF vs control.

    Journal: Gene expression patterns : GEP

    Article Title: Screen for Slit/Robo signaling in trunk neural cells reveals new players

    doi: 10.1016/j.gep.2018.01.002

    Figure Lengend Snippet: Microarray of Slit2 produced log ratios, expression and classification of genes. ( A ) Cartoon of a chicken embryo electroporation. In green we show the putative plasmid solution with fast green being injected inside the neural tube. And the ± shows the position of the electrodes when giving them the electric pulses necessary for the plasmid to enter cells in the NT. ( B ) Workflow and sample preparation and array processing. For the microarray we processed 6 Total RNA samples (1 Control, 2 experimental in duplicates) for gene expression. RNA quality and concentration was determined on the Agilent Bioanalyzer and NanoDrop spectrophotometer. One-color labeling reactions were prepared using the Agilent Two-Color Protocol (Version 6.6) with 400 ng total RNA input. ( C ) Log ratio values collected after microarray show significantly upregulated genes (red), significantly down regulated genes (green), and not differentially expressed (yellow). Control samples were labeled with Cy5 and Experimental sample labeled with Cy3. RNA Spike A mix was used for Cy3 (experimental) samples and RNA spike B mix was used for all Cy5 (Control) samples and scanned at 3 μM resolution on an Agilent G2565CA High Resolution Scanner. ( D ) Percentage of gene expression upon Slit2 gain of function in microarray. The bar graphed represents 37.3% down-regulation genes and 62.7% of incidents generated up-regulated genes of NCCs Slit2 GOF vs control.

    Article Snippet: Next, the labeled cRNA is fragmented and placed on the chicken 4×44K Gene Expression microarray and hybridized at 65 °C for ~17 h. Finally, the arrays are washed and scanned at 3 μM resolution on an Agilent G2565CA High Resolution Scanner.

    Techniques: Microarray, Produced, Expressing, Electroporation, Plasmid Preparation, Injection, Sample Prep, Concentration Assay, Spectrophotometry, Labeling, Generated

    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the microarray data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.

    Journal: Scientific Reports

    Article Title: Transcriptomics in lung tissue upon respiratory syncytial virus infection reveals aging as important modulator of immune activation and matrix maintenance

    doi: 10.1038/s41598-018-35180-2

    Figure Lengend Snippet: Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the microarray data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.

    Article Snippet: After washing (NimbleGen Arrays User’s Guide – Gene Expression Arrays Version 5.0), slides were scanned in an ozone-free room with a microarray scanner (Agilent DNA microarray scanner G2565CA, Agilent Technologies).

    Techniques: Expressing, Microarray, Derivative Assay, Mouse Assay

    Sox21 is a master regulator of normal keratinization in the cuticle of the hair shaft. ( A ) Summary of comparative microarray analyses of P5 back skin samples from WT, heterozygous, and homozygous mice. Gene ontology and expression profiling of the 119

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: The disruption of Sox21-mediated hair shaft cuticle differentiation causes cyclic alopecia in mice

    doi: 10.1073/pnas.0808324106

    Figure Lengend Snippet: Sox21 is a master regulator of normal keratinization in the cuticle of the hair shaft. ( A ) Summary of comparative microarray analyses of P5 back skin samples from WT, heterozygous, and homozygous mice. Gene ontology and expression profiling of the 119

    Article Snippet: Arrays were scanned with a microarray scanner system (G2565BA; Agilent) and the data were analyzed with Genespring GX software, version 7.3.1 (Agilent).

    Techniques: Microarray, Mouse Assay, Expressing

    NF-κB signaling analysis in THP-1 cells infected with SFTSV. The IPA platform was used to analyze components involved in the NF-κB response and their relationships and interactions. Fold changes of transcript expression levels of the genes in NF-κB signaling at 18 hpi were imported from the Agilent microarray analysis described above. Fold changes are shown in red (upregulation) and green (downregulation).

    Journal: Journal of Virology

    Article Title: Suppression of the Interferon and NF-?B Responses by Severe Fever with Thrombocytopenia Syndrome Virus

    doi: 10.1128/JVI.00612-12

    Figure Lengend Snippet: NF-κB signaling analysis in THP-1 cells infected with SFTSV. The IPA platform was used to analyze components involved in the NF-κB response and their relationships and interactions. Fold changes of transcript expression levels of the genes in NF-κB signaling at 18 hpi were imported from the Agilent microarray analysis described above. Fold changes are shown in red (upregulation) and green (downregulation).

    Article Snippet: Human 4x44K slides (Agilent) were used for hybridization, followed by scanning with an Agilent scanner (G2565BA).

    Techniques: Infection, Indirect Immunoperoxidase Assay, Expressing, Microarray

    Pathway analysis of the antiviral IFN response in infected THP-1 cells. The IPA platform was used to analyze components of the IFN response involved in both IRF and NF-κB signaling. Fold changes of transcript expression levels of the genes in IRF and NF-κB signaling at 18 hpi were imported from the Agilent microarray analysis. Fold changes are shown in red (upregulation) and green (downregulation).

    Journal: Journal of Virology

    Article Title: Suppression of the Interferon and NF-?B Responses by Severe Fever with Thrombocytopenia Syndrome Virus

    doi: 10.1128/JVI.00612-12

    Figure Lengend Snippet: Pathway analysis of the antiviral IFN response in infected THP-1 cells. The IPA platform was used to analyze components of the IFN response involved in both IRF and NF-κB signaling. Fold changes of transcript expression levels of the genes in IRF and NF-κB signaling at 18 hpi were imported from the Agilent microarray analysis. Fold changes are shown in red (upregulation) and green (downregulation).

    Article Snippet: Human 4x44K slides (Agilent) were used for hybridization, followed by scanning with an Agilent scanner (G2565BA).

    Techniques: Infection, Indirect Immunoperoxidase Assay, Expressing, Microarray

    Network associated genes differentially expressed in response to BPA. ( A ) “Endocrine System Disorders, Gastrointestinal Disease, Genetic Disorder” network and ( B ) “Cell Death, Cellular Growth and Proliferation, Cancer” network. The images were created using the IPA platform by overlaying the differentially expressed genes in response to BPA detected by Agilent microarray analysis onto a global molecular network from the Ingenuity knowledgebase. Red indicates upregulated genes, green indicates downregulated genes, and white indicates genes that were not annotated in this array but that form part of this network. The bottom numbers indicate the fold changes induced by BPA, and the top numbers are the P -values between the DMSO control group and the BPA treated group. Direct relationships are exhibited with solid arrows and indirect relationships with dashed arrows.

    Journal: PLoS ONE

    Article Title: Identification of Novel Low-Dose Bisphenol A Targets in Human Foreskin Fibroblast Cells Derived from Hypospadias Patients

    doi: 10.1371/journal.pone.0036711

    Figure Lengend Snippet: Network associated genes differentially expressed in response to BPA. ( A ) “Endocrine System Disorders, Gastrointestinal Disease, Genetic Disorder” network and ( B ) “Cell Death, Cellular Growth and Proliferation, Cancer” network. The images were created using the IPA platform by overlaying the differentially expressed genes in response to BPA detected by Agilent microarray analysis onto a global molecular network from the Ingenuity knowledgebase. Red indicates upregulated genes, green indicates downregulated genes, and white indicates genes that were not annotated in this array but that form part of this network. The bottom numbers indicate the fold changes induced by BPA, and the top numbers are the P -values between the DMSO control group and the BPA treated group. Direct relationships are exhibited with solid arrows and indirect relationships with dashed arrows.

    Article Snippet: The arrays were scanned using an Agilent Microarray Scanner (G2565BA, Agilent Technologies).

    Techniques: Indirect Immunoperoxidase Assay, Microarray

    Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of microarray probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic DNA extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.

    Journal: Nucleic Acids Research

    Article Title: Gene expression modulation is associated with gene amplification, supernumerary chromosomes and chromosome loss in antimony-resistant Leishmania infantum

    doi: 10.1093/nar/gkn1069

    Figure Lengend Snippet: Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of microarray probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic DNA extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.

    Article Snippet: Microarray data acquisition and analysis Detection of the Alexa Fluor 555 and Alexa Fluor 647 signals was performed on a G2565CA DNA microarray scanner (Agilent technologies) at a 5-μm resolution.

    Techniques: Transformation Assay, Expressing, Microarray, Southern Blot, Hybridization

    Gene expression profiling of marker genes in differentiated and undifferentiated MCT samples. Heat map and hierarchical clustering of all samples analyzed by DNA microarray using probes referable to the transcripts identified by class prediction analysis. Red indicates up-regulated and green down-regulated genes relative to the mean expression in all samples. For display purposes, samples in each class (differentiated and undifferentiated) were clustered separately and arranged from differentiated (left, yellow) to undifferentiated (right, red). Genes were hierarchically clustered separately based on the Pearson correlation coefficients and average linkage clustering. Units of the bar legend: absolute values.

    Journal: PLoS ONE

    Article Title: Global Gene Expression Analysis of Canine Cutaneous Mast Cell Tumor: Could Molecular Profiling Be Useful for Subtype Classification and Prognostication?

    doi: 10.1371/journal.pone.0095481

    Figure Lengend Snippet: Gene expression profiling of marker genes in differentiated and undifferentiated MCT samples. Heat map and hierarchical clustering of all samples analyzed by DNA microarray using probes referable to the transcripts identified by class prediction analysis. Red indicates up-regulated and green down-regulated genes relative to the mean expression in all samples. For display purposes, samples in each class (differentiated and undifferentiated) were clustered separately and arranged from differentiated (left, yellow) to undifferentiated (right, red). Genes were hierarchically clustered separately based on the Pearson correlation coefficients and average linkage clustering. Units of the bar legend: absolute values.

    Article Snippet: Hybridized slides were scanned at 5 µm resolution using a G2565BA DNA microarray scanner (Agilent Technologies, Santa Clara, CA).

    Techniques: Expressing, Marker, Microarray