full‐length cyp2d6 Search Results


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  • 99
    Thermo Fisher sybr green dye
    Sybr Green Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5629 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Pacific Biosciences cyp2d6
    Direct barcoding results. Direct barcoding results for the technical replicate with different barcodes. A : UCSC browser screenshot illustrating the detected variants (red lines) of the two fully‐phased haplogroup sequences for each sample barcode relative to the GRCh38 reference. The <t>CYP2D6</t> gene is located on the negative strand. Exon numbers are indicated in white based on the NM_000106.5 transcript sequence. For the haplogroup 2 sequences, the variants determining the CYP2D6 * 35 call are indicated. B : Identity and distribution of all 27 variants across the haplogroup sequences. Order in this table (top to bottom) is identical to those in Figure 2A (left to right). Variants in bold determine the CYP2D6 * 35 haplotype; * indicates that the variant was included on the Roche AmpliChip CYP450 test; # indicates that the variant was included in PharmGKB (February 4, 2016); and “HG” and “i” denote haplogroup and index, respectively.
    Cyp2d6, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 91/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Pacific Biosciences multiplex pcr primer guidelines
    Direct barcoding results. Direct barcoding results for the technical replicate with different barcodes. A : UCSC browser screenshot illustrating the detected variants (red lines) of the two fully‐phased haplogroup sequences for each sample barcode relative to the GRCh38 reference. The <t>CYP2D6</t> gene is located on the negative strand. Exon numbers are indicated in white based on the NM_000106.5 transcript sequence. For the haplogroup 2 sequences, the variants determining the CYP2D6 * 35 call are indicated. B : Identity and distribution of all 27 variants across the haplogroup sequences. Order in this table (top to bottom) is identical to those in Figure 2A (left to right). Variants in bold determine the CYP2D6 * 35 haplotype; * indicates that the variant was included on the Roche AmpliChip CYP450 test; # indicates that the variant was included in PharmGKB (February 4, 2016); and “HG” and “i” denote haplogroup and index, respectively.
    Multiplex Pcr Primer Guidelines, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 91/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Pacific Biosciences pacific biosciences rsii
    Direct barcoding results. Direct barcoding results for the technical replicate with different barcodes. A : UCSC browser screenshot illustrating the detected variants (red lines) of the two fully‐phased haplogroup sequences for each sample barcode relative to the GRCh38 reference. The <t>CYP2D6</t> gene is located on the negative strand. Exon numbers are indicated in white based on the NM_000106.5 transcript sequence. For the haplogroup 2 sequences, the variants determining the CYP2D6 * 35 call are indicated. B : Identity and distribution of all 27 variants across the haplogroup sequences. Order in this table (top to bottom) is identical to those in Figure 2A (left to right). Variants in bold determine the CYP2D6 * 35 haplotype; * indicates that the variant was included on the Roche AmpliChip CYP450 test; # indicates that the variant was included in PharmGKB (February 4, 2016); and “HG” and “i” denote haplogroup and index, respectively.
    Pacific Biosciences Rsii, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 89/100, based on 246 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Thermo Fisher taqman probes
    Direct barcoding results. Direct barcoding results for the technical replicate with different barcodes. A : UCSC browser screenshot illustrating the detected variants (red lines) of the two fully‐phased haplogroup sequences for each sample barcode relative to the GRCh38 reference. The <t>CYP2D6</t> gene is located on the negative strand. Exon numbers are indicated in white based on the NM_000106.5 transcript sequence. For the haplogroup 2 sequences, the variants determining the CYP2D6 * 35 call are indicated. B : Identity and distribution of all 27 variants across the haplogroup sequences. Order in this table (top to bottom) is identical to those in Figure 2A (left to right). Variants in bold determine the CYP2D6 * 35 haplotype; * indicates that the variant was included on the Roche AmpliChip CYP450 test; # indicates that the variant was included in PharmGKB (February 4, 2016); and “HG” and “i” denote haplogroup and index, respectively.
    Taqman Probes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 30308 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher abi prism 7300 sequence detection system
    Direct barcoding results. Direct barcoding results for the technical replicate with different barcodes. A : UCSC browser screenshot illustrating the detected variants (red lines) of the two fully‐phased haplogroup sequences for each sample barcode relative to the GRCh38 reference. The <t>CYP2D6</t> gene is located on the negative strand. Exon numbers are indicated in white based on the NM_000106.5 transcript sequence. For the haplogroup 2 sequences, the variants determining the CYP2D6 * 35 call are indicated. B : Identity and distribution of all 27 variants across the haplogroup sequences. Order in this table (top to bottom) is identical to those in Figure 2A (left to right). Variants in bold determine the CYP2D6 * 35 haplotype; * indicates that the variant was included on the Roche AmpliChip CYP450 test; # indicates that the variant was included in PharmGKB (February 4, 2016); and “HG” and “i” denote haplogroup and index, respectively.
    Abi Prism 7300 Sequence Detection System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 7780 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Pacific Biosciences smrt sequencing technique
    Direct barcoding results. Direct barcoding results for the technical replicate with different barcodes. A : UCSC browser screenshot illustrating the detected variants (red lines) of the two fully‐phased haplogroup sequences for each sample barcode relative to the GRCh38 reference. The <t>CYP2D6</t> gene is located on the negative strand. Exon numbers are indicated in white based on the NM_000106.5 transcript sequence. For the haplogroup 2 sequences, the variants determining the CYP2D6 * 35 call are indicated. B : Identity and distribution of all 27 variants across the haplogroup sequences. Order in this table (top to bottom) is identical to those in Figure 2A (left to right). Variants in bold determine the CYP2D6 * 35 haplotype; * indicates that the variant was included on the Roche AmpliChip CYP450 test; # indicates that the variant was included in PharmGKB (February 4, 2016); and “HG” and “i” denote haplogroup and index, respectively.
    Smrt Sequencing Technique, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    TaKaRa takara la taq
    Direct barcoding results. Direct barcoding results for the technical replicate with different barcodes. A : UCSC browser screenshot illustrating the detected variants (red lines) of the two fully‐phased haplogroup sequences for each sample barcode relative to the GRCh38 reference. The <t>CYP2D6</t> gene is located on the negative strand. Exon numbers are indicated in white based on the NM_000106.5 transcript sequence. For the haplogroup 2 sequences, the variants determining the CYP2D6 * 35 call are indicated. B : Identity and distribution of all 27 variants across the haplogroup sequences. Order in this table (top to bottom) is identical to those in Figure 2A (left to right). Variants in bold determine the CYP2D6 * 35 haplotype; * indicates that the variant was included on the Roche AmpliChip CYP450 test; # indicates that the variant was included in PharmGKB (February 4, 2016); and “HG” and “i” denote haplogroup and index, respectively.
    Takara La Taq, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 3559 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Pacific Biosciences amplicon pacbio sequencing
    Direct barcoding results. Direct barcoding results for the technical replicate with different barcodes. A : UCSC browser screenshot illustrating the detected variants (red lines) of the two fully‐phased haplogroup sequences for each sample barcode relative to the GRCh38 reference. The <t>CYP2D6</t> gene is located on the negative strand. Exon numbers are indicated in white based on the NM_000106.5 transcript sequence. For the haplogroup 2 sequences, the variants determining the CYP2D6 * 35 call are indicated. B : Identity and distribution of all 27 variants across the haplogroup sequences. Order in this table (top to bottom) is identical to those in Figure 2A (left to right). Variants in bold determine the CYP2D6 * 35 haplotype; * indicates that the variant was included on the Roche AmpliChip CYP450 test; # indicates that the variant was included in PharmGKB (February 4, 2016); and “HG” and “i” denote haplogroup and index, respectively.
    Amplicon Pacbio Sequencing, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Mimotopes fmoc solid phase chemistry
    Direct barcoding results. Direct barcoding results for the technical replicate with different barcodes. A : UCSC browser screenshot illustrating the detected variants (red lines) of the two fully‐phased haplogroup sequences for each sample barcode relative to the GRCh38 reference. The <t>CYP2D6</t> gene is located on the negative strand. Exon numbers are indicated in white based on the NM_000106.5 transcript sequence. For the haplogroup 2 sequences, the variants determining the CYP2D6 * 35 call are indicated. B : Identity and distribution of all 27 variants across the haplogroup sequences. Order in this table (top to bottom) is identical to those in Figure 2A (left to right). Variants in bold determine the CYP2D6 * 35 haplotype; * indicates that the variant was included on the Roche AmpliChip CYP450 test; # indicates that the variant was included in PharmGKB (February 4, 2016); and “HG” and “i” denote haplogroup and index, respectively.
    Fmoc Solid Phase Chemistry, supplied by Mimotopes, used in various techniques. Bioz Stars score: 89/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Cell Signaling Technology Inc antibody against caspase 3
    <t>Caspase-3</t> activation is involved in the MA and/or gp120-mediated apoptosis in astrocytes. ( a and b ) SVGA cells were treated with MA and/or gp120 for 24 h and the cytoplasmic proteins were obtained, which were used to measure procaspase-3 (35 kDa) and cleaved caspase-3 (17 kDa) protein expressions ( a ) and caspase-3 cleavage activity ( b ). The cells were also treated with either 100 μ M vit. C ( c ), 25 μ M DAS ( d ), 50 nM DPI ( e ), or 50 nM DFO ( f ) 1 h before the treatment with MA and/or gp120 and the effect on caspase-3 cleavage activity was measured. The activity was normalized with basal levels in untreated control and reported as change in the activity. The bars represent mean±S.E. of three independent experiments with each treatment in triplicates. The P -value ≤0.05 (*) and ≤0.01(**) were considered statistically significant using two-tailed Student's t -test and multiple analysis of variance (ANOVA). The significant values shown above the bars were calculated using Student's t -test for comparison between the treatment and mock-transfected control
    Antibody Against Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 3636 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Taconic Biosciences cyp2d kos
    <t>Caspase-3</t> activation is involved in the MA and/or gp120-mediated apoptosis in astrocytes. ( a and b ) SVGA cells were treated with MA and/or gp120 for 24 h and the cytoplasmic proteins were obtained, which were used to measure procaspase-3 (35 kDa) and cleaved caspase-3 (17 kDa) protein expressions ( a ) and caspase-3 cleavage activity ( b ). The cells were also treated with either 100 μ M vit. C ( c ), 25 μ M DAS ( d ), 50 nM DPI ( e ), or 50 nM DFO ( f ) 1 h before the treatment with MA and/or gp120 and the effect on caspase-3 cleavage activity was measured. The activity was normalized with basal levels in untreated control and reported as change in the activity. The bars represent mean±S.E. of three independent experiments with each treatment in triplicates. The P -value ≤0.05 (*) and ≤0.01(**) were considered statistically significant using two-tailed Student's t -test and multiple analysis of variance (ANOVA). The significant values shown above the bars were calculated using Student's t -test for comparison between the treatment and mock-transfected control
    Cyp2d Kos, supplied by Taconic Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc p53
    Cotreatment with Res and Gef affects the expression of apoptosis-, autophagy- and senescence-related proteins in PC9/G cells. Cells were treated with Gef (1 μM) alone or combined with Res (40 μM) for 72 h. Then, the expression levels of cleaved caspase-3, LC3B-II, beclin 1, <t>p53,</t> and p21 protein were analysed by Western blotting. Data are presented as means ± SD (n = 3). * P
    P53, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 8090 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc mouse anti caspase 7
    Risperidone has no influence on tamoxifen-induced cleavage of caspases and PARP-1 in T47D cells. Cells were treated with 1 µM tamoxifen with or without 3 µM risperidone or 0.3 µM fluoxetine for 72 hours. Representative protein blotting images are shown in ( A ). Treatment of tamoxifen with or without risperidone resulted in increased protein expression of cleaved caspase 9 ( B ), caspase 7 ( C ), caspase 3 ( D ), and PARP-1 ( E ). Graphs show mean ± SEM of three or more independent experiments.  * ,  p
    Mouse Anti Caspase 7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher gene exp cyp2d6 hs02576167 m1
    Identification of genetic variants of <t>CYP2D6</t> with mutations in putative targeting signals. (A and B) Total RNA was extracted from human liver samples and the first 600 nucleotides of the coding region were amplified by RT-PCR. The amplified DNA was cloned and sequenced. (A) Schematic representations of point mutations identified in the human liver samples. (B) Schematic representations of splice variants identified. The deleted or skipped exon regions are shown as hatched boxes.
    Gene Exp Cyp2d6 Hs02576167 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    PerkinElmer cyp2d6 f
    Electrophoresis of PCR products from genomic DNA amplified using <t>CYP2D6</t> F and CYP2D6 4R primers. Lane 1: λ/EcoR I and Hind III marker, lanes 2-4: PCR products from livers expressing full and short length RT-PCR products, Lanes 5, 6: PCR products from livers expressing full length RT-PCR products, Lanes 7, 8: PCR products from livers expressing only short length RT-PCR products.
    Cyp2d6 F, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Pacific Biosciences unique barcodes
    Direct barcoding results. Direct barcoding results for the technical replicate with different <t>barcodes.</t> A : UCSC browser screenshot illustrating the detected variants (red lines) of the two fully‐phased haplogroup sequences for each sample barcode relative to the GRCh38 reference. The CYP2D6 gene is located on the negative strand. Exon numbers are indicated in white based on the NM_000106.5 transcript sequence. For the haplogroup 2 sequences, the variants determining the CYP2D6 * 35 call are indicated. B : Identity and distribution of all 27 variants across the haplogroup sequences. Order in this table (top to bottom) is identical to those in Figure 2A (left to right). Variants in bold determine the CYP2D6 * 35 haplotype; * indicates that the variant was included on the Roche AmpliChip CYP450 test; # indicates that the variant was included in PharmGKB (February 4, 2016); and “HG” and “i” denote haplogroup and index, respectively.
    Unique Barcodes, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 91/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Pacific Biosciences pacbio smrt cell
    Direct barcoding results. Direct barcoding results for the technical replicate with different <t>barcodes.</t> A : UCSC browser screenshot illustrating the detected variants (red lines) of the two fully‐phased haplogroup sequences for each sample barcode relative to the GRCh38 reference. The CYP2D6 gene is located on the negative strand. Exon numbers are indicated in white based on the NM_000106.5 transcript sequence. For the haplogroup 2 sequences, the variants determining the CYP2D6 * 35 call are indicated. B : Identity and distribution of all 27 variants across the haplogroup sequences. Order in this table (top to bottom) is identical to those in Figure 2A (left to right). Variants in bold determine the CYP2D6 * 35 haplotype; * indicates that the variant was included on the Roche AmpliChip CYP450 test; # indicates that the variant was included in PharmGKB (February 4, 2016); and “HG” and “i” denote haplogroup and index, respectively.
    Pacbio Smrt Cell, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 88/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Integrated DNA Technologies supplementary data
    Direct barcoding results. Direct barcoding results for the technical replicate with different <t>barcodes.</t> A : UCSC browser screenshot illustrating the detected variants (red lines) of the two fully‐phased haplogroup sequences for each sample barcode relative to the GRCh38 reference. The CYP2D6 gene is located on the negative strand. Exon numbers are indicated in white based on the NM_000106.5 transcript sequence. For the haplogroup 2 sequences, the variants determining the CYP2D6 * 35 call are indicated. B : Identity and distribution of all 27 variants across the haplogroup sequences. Order in this table (top to bottom) is identical to those in Figure 2A (left to right). Variants in bold determine the CYP2D6 * 35 haplotype; * indicates that the variant was included on the Roche AmpliChip CYP450 test; # indicates that the variant was included in PharmGKB (February 4, 2016); and “HG” and “i” denote haplogroup and index, respectively.
    Supplementary Data, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen qiaamp dna mini kit
    Direct barcoding results. Direct barcoding results for the technical replicate with different <t>barcodes.</t> A : UCSC browser screenshot illustrating the detected variants (red lines) of the two fully‐phased haplogroup sequences for each sample barcode relative to the GRCh38 reference. The CYP2D6 gene is located on the negative strand. Exon numbers are indicated in white based on the NM_000106.5 transcript sequence. For the haplogroup 2 sequences, the variants determining the CYP2D6 * 35 call are indicated. B : Identity and distribution of all 27 variants across the haplogroup sequences. Order in this table (top to bottom) is identical to those in Figure 2A (left to right). Variants in bold determine the CYP2D6 * 35 haplotype; * indicates that the variant was included on the Roche AmpliChip CYP450 test; # indicates that the variant was included in PharmGKB (February 4, 2016); and “HG” and “i” denote haplogroup and index, respectively.
    Qiaamp Dna Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 48891 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Roche unphased diplotypes
    Direct barcoding results. Direct barcoding results for the technical replicate with different <t>barcodes.</t> A : UCSC browser screenshot illustrating the detected variants (red lines) of the two fully‐phased haplogroup sequences for each sample barcode relative to the GRCh38 reference. The CYP2D6 gene is located on the negative strand. Exon numbers are indicated in white based on the NM_000106.5 transcript sequence. For the haplogroup 2 sequences, the variants determining the CYP2D6 * 35 call are indicated. B : Identity and distribution of all 27 variants across the haplogroup sequences. Order in this table (top to bottom) is identical to those in Figure 2A (left to right). Variants in bold determine the CYP2D6 * 35 haplotype; * indicates that the variant was included on the Roche AmpliChip CYP450 test; # indicates that the variant was included in PharmGKB (February 4, 2016); and “HG” and “i” denote haplogroup and index, respectively.
    Unphased Diplotypes, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa takara la taq dna polymerase
    Direct barcoding results. Direct barcoding results for the technical replicate with different <t>barcodes.</t> A : UCSC browser screenshot illustrating the detected variants (red lines) of the two fully‐phased haplogroup sequences for each sample barcode relative to the GRCh38 reference. The CYP2D6 gene is located on the negative strand. Exon numbers are indicated in white based on the NM_000106.5 transcript sequence. For the haplogroup 2 sequences, the variants determining the CYP2D6 * 35 call are indicated. B : Identity and distribution of all 27 variants across the haplogroup sequences. Order in this table (top to bottom) is identical to those in Figure 2A (left to right). Variants in bold determine the CYP2D6 * 35 haplotype; * indicates that the variant was included on the Roche AmpliChip CYP450 test; # indicates that the variant was included in PharmGKB (February 4, 2016); and “HG” and “i” denote haplogroup and index, respectively.
    Takara La Taq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher cdna archive kit
    Direct barcoding results. Direct barcoding results for the technical replicate with different <t>barcodes.</t> A : UCSC browser screenshot illustrating the detected variants (red lines) of the two fully‐phased haplogroup sequences for each sample barcode relative to the GRCh38 reference. The CYP2D6 gene is located on the negative strand. Exon numbers are indicated in white based on the NM_000106.5 transcript sequence. For the haplogroup 2 sequences, the variants determining the CYP2D6 * 35 call are indicated. B : Identity and distribution of all 27 variants across the haplogroup sequences. Order in this table (top to bottom) is identical to those in Figure 2A (left to right). Variants in bold determine the CYP2D6 * 35 haplotype; * indicates that the variant was included on the Roche AmpliChip CYP450 test; # indicates that the variant was included in PharmGKB (February 4, 2016); and “HG” and “i” denote haplogroup and index, respectively.
    Cdna Archive Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1141 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Technelysium version 2 6 5
    Direct barcoding results. Direct barcoding results for the technical replicate with different <t>barcodes.</t> A : UCSC browser screenshot illustrating the detected variants (red lines) of the two fully‐phased haplogroup sequences for each sample barcode relative to the GRCh38 reference. The CYP2D6 gene is located on the negative strand. Exon numbers are indicated in white based on the NM_000106.5 transcript sequence. For the haplogroup 2 sequences, the variants determining the CYP2D6 * 35 call are indicated. B : Identity and distribution of all 27 variants across the haplogroup sequences. Order in this table (top to bottom) is identical to those in Figure 2A (left to right). Variants in bold determine the CYP2D6 * 35 haplotype; * indicates that the variant was included on the Roche AmpliChip CYP450 test; # indicates that the variant was included in PharmGKB (February 4, 2016); and “HG” and “i” denote haplogroup and index, respectively.
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    Direct barcoding results. Direct barcoding results for the technical replicate with different barcodes. A : UCSC browser screenshot illustrating the detected variants (red lines) of the two fully‐phased haplogroup sequences for each sample barcode relative to the GRCh38 reference. The CYP2D6 gene is located on the negative strand. Exon numbers are indicated in white based on the NM_000106.5 transcript sequence. For the haplogroup 2 sequences, the variants determining the CYP2D6 * 35 call are indicated. B : Identity and distribution of all 27 variants across the haplogroup sequences. Order in this table (top to bottom) is identical to those in Figure 2A (left to right). Variants in bold determine the CYP2D6 * 35 haplotype; * indicates that the variant was included on the Roche AmpliChip CYP450 test; # indicates that the variant was included in PharmGKB (February 4, 2016); and “HG” and “i” denote haplogroup and index, respectively.

    Journal: Human Mutation

    Article Title: Flexible and Scalable Full‐Length CYP2D6 Long Amplicon PacBio Sequencing

    doi: 10.1002/humu.23166

    Figure Lengend Snippet: Direct barcoding results. Direct barcoding results for the technical replicate with different barcodes. A : UCSC browser screenshot illustrating the detected variants (red lines) of the two fully‐phased haplogroup sequences for each sample barcode relative to the GRCh38 reference. The CYP2D6 gene is located on the negative strand. Exon numbers are indicated in white based on the NM_000106.5 transcript sequence. For the haplogroup 2 sequences, the variants determining the CYP2D6 * 35 call are indicated. B : Identity and distribution of all 27 variants across the haplogroup sequences. Order in this table (top to bottom) is identical to those in Figure 2A (left to right). Variants in bold determine the CYP2D6 * 35 haplotype; * indicates that the variant was included on the Roche AmpliChip CYP450 test; # indicates that the variant was included in PharmGKB (February 4, 2016); and “HG” and “i” denote haplogroup and index, respectively.

    Article Snippet: The technical replicate was in full agreement with haplogroup 2 for barcode 1 except for an one nucleotide length difference in a 22‐bp T‐homopolymer located in the upstream region of CYP2D6 (22:g.42132029delT) for haplogroup 2.

    Techniques: Sequencing, Variant Assay

    Caspase-3 activation is involved in the MA and/or gp120-mediated apoptosis in astrocytes. ( a and b ) SVGA cells were treated with MA and/or gp120 for 24 h and the cytoplasmic proteins were obtained, which were used to measure procaspase-3 (35 kDa) and cleaved caspase-3 (17 kDa) protein expressions ( a ) and caspase-3 cleavage activity ( b ). The cells were also treated with either 100 μ M vit. C ( c ), 25 μ M DAS ( d ), 50 nM DPI ( e ), or 50 nM DFO ( f ) 1 h before the treatment with MA and/or gp120 and the effect on caspase-3 cleavage activity was measured. The activity was normalized with basal levels in untreated control and reported as change in the activity. The bars represent mean±S.E. of three independent experiments with each treatment in triplicates. The P -value ≤0.05 (*) and ≤0.01(**) were considered statistically significant using two-tailed Student's t -test and multiple analysis of variance (ANOVA). The significant values shown above the bars were calculated using Student's t -test for comparison between the treatment and mock-transfected control

    Journal: Cell Death & Disease

    Article Title: HIV gp120- and methamphetamine-mediated oxidative stress induces astrocyte apoptosis via cytochrome P450 2E1

    doi: 10.1038/cddis.2013.374

    Figure Lengend Snippet: Caspase-3 activation is involved in the MA and/or gp120-mediated apoptosis in astrocytes. ( a and b ) SVGA cells were treated with MA and/or gp120 for 24 h and the cytoplasmic proteins were obtained, which were used to measure procaspase-3 (35 kDa) and cleaved caspase-3 (17 kDa) protein expressions ( a ) and caspase-3 cleavage activity ( b ). The cells were also treated with either 100 μ M vit. C ( c ), 25 μ M DAS ( d ), 50 nM DPI ( e ), or 50 nM DFO ( f ) 1 h before the treatment with MA and/or gp120 and the effect on caspase-3 cleavage activity was measured. The activity was normalized with basal levels in untreated control and reported as change in the activity. The bars represent mean±S.E. of three independent experiments with each treatment in triplicates. The P -value ≤0.05 (*) and ≤0.01(**) were considered statistically significant using two-tailed Student's t -test and multiple analysis of variance (ANOVA). The significant values shown above the bars were calculated using Student's t -test for comparison between the treatment and mock-transfected control

    Article Snippet: Specific antibodies against ferritin heavy chain (B12), CYP2E1 (H-21), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (FL-335) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), antibody against caspase-3, which detects procaspase3 (35 kDa) and cleaved caspase-3 (17 kDa) was purchased from Cell Signaling Technology (Danvers, MA, USA) and antibody against CYP2D6 and CYP2B6 were purchased from Abcam (Cambridge, MA, USA).

    Techniques: Activation Assay, Activity Assay, Two Tailed Test, Transfection

    Schematic illustration of MA- and/or gp120-mediated oxidative stress. MA and/or gp120 increase the expressions of CYP2E1, which generates free electrons via conversion of NADPH to NADP + . The CYP2E1 reaction cycle produces ROS as a result of uncoupling of the reaction. In addition, the NADP + generated during this process is recycled via NOX to produce superoxides and peroxides. The superoxides can further generate peroxides through Fenton chemistry. The ROS generated via this mechanism increases the caspase-3 activity, which then induces DNA fragmentation, ultimately leading to apoptosis in astrocytes. The combination of MA and gp120 thus exacerbates the toxicity through common molecular mechanism(s). R-H represents MA or physiological substrates, such as dopamine. R-H may be oxidized to R-OH, which may further be oxidized to R-(OH) 2 or R-CHO

    Journal: Cell Death & Disease

    Article Title: HIV gp120- and methamphetamine-mediated oxidative stress induces astrocyte apoptosis via cytochrome P450 2E1

    doi: 10.1038/cddis.2013.374

    Figure Lengend Snippet: Schematic illustration of MA- and/or gp120-mediated oxidative stress. MA and/or gp120 increase the expressions of CYP2E1, which generates free electrons via conversion of NADPH to NADP + . The CYP2E1 reaction cycle produces ROS as a result of uncoupling of the reaction. In addition, the NADP + generated during this process is recycled via NOX to produce superoxides and peroxides. The superoxides can further generate peroxides through Fenton chemistry. The ROS generated via this mechanism increases the caspase-3 activity, which then induces DNA fragmentation, ultimately leading to apoptosis in astrocytes. The combination of MA and gp120 thus exacerbates the toxicity through common molecular mechanism(s). R-H represents MA or physiological substrates, such as dopamine. R-H may be oxidized to R-OH, which may further be oxidized to R-(OH) 2 or R-CHO

    Article Snippet: Specific antibodies against ferritin heavy chain (B12), CYP2E1 (H-21), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (FL-335) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), antibody against caspase-3, which detects procaspase3 (35 kDa) and cleaved caspase-3 (17 kDa) was purchased from Cell Signaling Technology (Danvers, MA, USA) and antibody against CYP2D6 and CYP2B6 were purchased from Abcam (Cambridge, MA, USA).

    Techniques: Generated, Activity Assay

    Cotreatment with Res and Gef affects the expression of apoptosis-, autophagy- and senescence-related proteins in PC9/G cells. Cells were treated with Gef (1 μM) alone or combined with Res (40 μM) for 72 h. Then, the expression levels of cleaved caspase-3, LC3B-II, beclin 1, p53, and p21 protein were analysed by Western blotting. Data are presented as means ± SD (n = 3). * P

    Journal: Scientific Reports

    Article Title: Resveratrol overcomes gefitinib resistance by increasing the intracellular gefitinib concentration and triggering apoptosis, autophagy and senescence in PC9/G NSCLC cells

    doi: 10.1038/srep17730

    Figure Lengend Snippet: Cotreatment with Res and Gef affects the expression of apoptosis-, autophagy- and senescence-related proteins in PC9/G cells. Cells were treated with Gef (1 μM) alone or combined with Res (40 μM) for 72 h. Then, the expression levels of cleaved caspase-3, LC3B-II, beclin 1, p53, and p21 protein were analysed by Western blotting. Data are presented as means ± SD (n = 3). * P

    Article Snippet: Primary antibodies against EGFR, phospho-EGFR (pY1068 -EGFR), p21waf1/cip1 , p53, LC3B, caspase-3, beclin-1, and β-actin were obtained from Cell Signalling Technology (Beverly, MA, USA).

    Techniques: Expressing, Western Blot

    Cotreatment with Res and Gef affects the expression of apoptosis-, autophagy- and senescence-related proteins in PC9/G cells. Cells were treated with Gef (1 μM) alone or combined with Res (40 μM) for 72 h. Then, the expression levels of cleaved caspase-3, LC3B-II, beclin 1, p53, and p21 protein were analysed by Western blotting. Data are presented as means ± SD (n = 3). * P

    Journal: Scientific Reports

    Article Title: Resveratrol overcomes gefitinib resistance by increasing the intracellular gefitinib concentration and triggering apoptosis, autophagy and senescence in PC9/G NSCLC cells

    doi: 10.1038/srep17730

    Figure Lengend Snippet: Cotreatment with Res and Gef affects the expression of apoptosis-, autophagy- and senescence-related proteins in PC9/G cells. Cells were treated with Gef (1 μM) alone or combined with Res (40 μM) for 72 h. Then, the expression levels of cleaved caspase-3, LC3B-II, beclin 1, p53, and p21 protein were analysed by Western blotting. Data are presented as means ± SD (n = 3). * P

    Article Snippet: Primary antibodies against EGFR, phospho-EGFR (pY1068 -EGFR), p21waf1/cip1 , p53, LC3B, caspase-3, beclin-1, and β-actin were obtained from Cell Signalling Technology (Beverly, MA, USA).

    Techniques: Expressing, Western Blot

    Risperidone has no influence on tamoxifen-induced cleavage of caspases and PARP-1 in T47D cells. Cells were treated with 1 µM tamoxifen with or without 3 µM risperidone or 0.3 µM fluoxetine for 72 hours. Representative protein blotting images are shown in ( A ). Treatment of tamoxifen with or without risperidone resulted in increased protein expression of cleaved caspase 9 ( B ), caspase 7 ( C ), caspase 3 ( D ), and PARP-1 ( E ). Graphs show mean ± SEM of three or more independent experiments.  * ,  p

    Journal: PLoS ONE

    Article Title: Combination Treatment of Tamoxifen with Risperidone in Breast Cancer

    doi: 10.1371/journal.pone.0098805

    Figure Lengend Snippet: Risperidone has no influence on tamoxifen-induced cleavage of caspases and PARP-1 in T47D cells. Cells were treated with 1 µM tamoxifen with or without 3 µM risperidone or 0.3 µM fluoxetine for 72 hours. Representative protein blotting images are shown in ( A ). Treatment of tamoxifen with or without risperidone resulted in increased protein expression of cleaved caspase 9 ( B ), caspase 7 ( C ), caspase 3 ( D ), and PARP-1 ( E ). Graphs show mean ± SEM of three or more independent experiments. * , p

    Article Snippet: Mouse anti-caspase-7, anti-mouse, anti-rabbit, and anti-goat horseradish peroxidase (HRP)-linked antibodies were purchased from Cell Signaling (Danvers, MA).

    Techniques: Expressing

    Identification of genetic variants of CYP2D6 with mutations in putative targeting signals. (A and B) Total RNA was extracted from human liver samples and the first 600 nucleotides of the coding region were amplified by RT-PCR. The amplified DNA was cloned and sequenced. (A) Schematic representations of point mutations identified in the human liver samples. (B) Schematic representations of splice variants identified. The deleted or skipped exon regions are shown as hatched boxes.

    Journal: Molecular genetics and metabolism

    Article Title: Identification of Genetic Variants of Human Cytochrome P450 2D6 with Impaired Mitochondrial Targeting

    doi: 10.1016/j.ymgme.2009.08.009

    Figure Lengend Snippet: Identification of genetic variants of CYP2D6 with mutations in putative targeting signals. (A and B) Total RNA was extracted from human liver samples and the first 600 nucleotides of the coding region were amplified by RT-PCR. The amplified DNA was cloned and sequenced. (A) Schematic representations of point mutations identified in the human liver samples. (B) Schematic representations of splice variants identified. The deleted or skipped exon regions are shown as hatched boxes.

    Article Snippet: A TaqMan gene expression assay that was designed to detect just the exon 3-skipped splice variant form (Hs02576167_m1, Applied Biosystems) was used.

    Techniques: Amplification, Reverse Transcription Polymerase Chain Reaction, Clone Assay

    Electrophoresis of PCR products from genomic DNA amplified using CYP2D6 F and CYP2D6 4R primers. Lane 1: λ/EcoR I and Hind III marker, lanes 2-4: PCR products from livers expressing full and short length RT-PCR products, Lanes 5, 6: PCR products from livers expressing full length RT-PCR products, Lanes 7, 8: PCR products from livers expressing only short length RT-PCR products.

    Journal: World Journal of Gastroenterology : WJG

    Article Title: Three new alternative splicing variants of human cytochrome P450 2D6 mRNA in human extratumoral liver tissue

    doi: 10.3748/wjg.v10.i22.3356

    Figure Lengend Snippet: Electrophoresis of PCR products from genomic DNA amplified using CYP2D6 F and CYP2D6 4R primers. Lane 1: λ/EcoR I and Hind III marker, lanes 2-4: PCR products from livers expressing full and short length RT-PCR products, Lanes 5, 6: PCR products from livers expressing full length RT-PCR products, Lanes 7, 8: PCR products from livers expressing only short length RT-PCR products.

    Article Snippet: The segment of CYP2D6 gene from exons 1 to 4 was amplified with CYP2D6 F and CYP2D6 4R primers.

    Techniques: Electrophoresis, Polymerase Chain Reaction, Amplification, Marker, Expressing, Reverse Transcription Polymerase Chain Reaction

    Direct barcoding results. Direct barcoding results for the technical replicate with different barcodes. A : UCSC browser screenshot illustrating the detected variants (red lines) of the two fully‐phased haplogroup sequences for each sample barcode relative to the GRCh38 reference. The CYP2D6 gene is located on the negative strand. Exon numbers are indicated in white based on the NM_000106.5 transcript sequence. For the haplogroup 2 sequences, the variants determining the CYP2D6 * 35 call are indicated. B : Identity and distribution of all 27 variants across the haplogroup sequences. Order in this table (top to bottom) is identical to those in Figure 2A (left to right). Variants in bold determine the CYP2D6 * 35 haplotype; * indicates that the variant was included on the Roche AmpliChip CYP450 test; # indicates that the variant was included in PharmGKB (February 4, 2016); and “HG” and “i” denote haplogroup and index, respectively.

    Journal: Human Mutation

    Article Title: Flexible and Scalable Full‐Length CYP2D6 Long Amplicon PacBio Sequencing

    doi: 10.1002/humu.23166

    Figure Lengend Snippet: Direct barcoding results. Direct barcoding results for the technical replicate with different barcodes. A : UCSC browser screenshot illustrating the detected variants (red lines) of the two fully‐phased haplogroup sequences for each sample barcode relative to the GRCh38 reference. The CYP2D6 gene is located on the negative strand. Exon numbers are indicated in white based on the NM_000106.5 transcript sequence. For the haplogroup 2 sequences, the variants determining the CYP2D6 * 35 call are indicated. B : Identity and distribution of all 27 variants across the haplogroup sequences. Order in this table (top to bottom) is identical to those in Figure 2A (left to right). Variants in bold determine the CYP2D6 * 35 haplotype; * indicates that the variant was included on the Roche AmpliChip CYP450 test; # indicates that the variant was included in PharmGKB (February 4, 2016); and “HG” and “i” denote haplogroup and index, respectively.

    Article Snippet: Initial analysis of one individual was performed using two technical replicate libraries with unique barcodes, prepared and sequenced together on one PacBio SMRT cell.

    Techniques: Sequencing, Variant Assay

    Barcoding schemes. Direct versus two‐step sample barcoding. A : In the direct barcoding scheme the sample specific barcodes, indicated by the blue and red for patients 1 and 2, respectively, are attached to the gene‐specific sequences (black arrows) and are introduced in a single PCR reaction. B : For the two‐step procedure for each individual, the region of interest is first amplified with a pair of gene‐specific primers with M13 forward (green) and reverse (purple) sequence tails. A symmetrical sample barcode, indicated by blue and red for patients 1 and 2, respectively, is introduced in a second PCR using a set of M13 barcode primers. A 5' padding sequence (black) is present on the index primers for both the direct and two‐step barcoding schemes to give all fragment identical end sequences to avoid ligation biases during the SMRT bell library preparation.

    Journal: Human Mutation

    Article Title: Flexible and Scalable Full‐Length CYP2D6 Long Amplicon PacBio Sequencing

    doi: 10.1002/humu.23166

    Figure Lengend Snippet: Barcoding schemes. Direct versus two‐step sample barcoding. A : In the direct barcoding scheme the sample specific barcodes, indicated by the blue and red for patients 1 and 2, respectively, are attached to the gene‐specific sequences (black arrows) and are introduced in a single PCR reaction. B : For the two‐step procedure for each individual, the region of interest is first amplified with a pair of gene‐specific primers with M13 forward (green) and reverse (purple) sequence tails. A symmetrical sample barcode, indicated by blue and red for patients 1 and 2, respectively, is introduced in a second PCR using a set of M13 barcode primers. A 5' padding sequence (black) is present on the index primers for both the direct and two‐step barcoding schemes to give all fragment identical end sequences to avoid ligation biases during the SMRT bell library preparation.

    Article Snippet: Initial analysis of one individual was performed using two technical replicate libraries with unique barcodes, prepared and sequenced together on one PacBio SMRT cell.

    Techniques: Polymerase Chain Reaction, Amplification, Sequencing, Ligation