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  • 94
    Promega fugene hd reagent
    Fugene Hd Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 1981 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugene hd reagent/product/Promega
    Average 94 stars, based on 1981 article reviews
    Price from $9.99 to $1999.99
    fugene hd reagent - by Bioz Stars, 2020-07
    94/100 stars
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    99
    Promega hd transfection fugene hd reagent
    Hd Transfection Fugene Hd Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 512 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hd transfection fugene hd reagent/product/Promega
    Average 99 stars, based on 512 article reviews
    Price from $9.99 to $1999.99
    hd transfection fugene hd reagent - by Bioz Stars, 2020-07
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    94
    Roche fugene hd reagent
    DNA transfection reagent enhances internalization of multiple <t>BoNT</t> Lc serotypes in two neuroblastoma cell lines. M17 or N2A cells were exposed for 24 hrs to 50, 10 or 2 nM of BoNT/E toxin (A) or 30 or 6 nM of GST-Lc/E (B) or 50 or 10 nM of BoNT/B toxin (C) or 150 or 30 nM of recombinant Lc/B (D), + or − pre-incubation with <t>FuGene-HD.</t> Cell extracts were prepared and the extent of BoNT intoxication was assessed by Western blotting to monitor SNAP25 or VAMP2 cleavage.
    Fugene Hd Reagent, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 2769 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugene hd reagent/product/Roche
    Average 94 stars, based on 2769 article reviews
    Price from $9.99 to $1999.99
    fugene hd reagent - by Bioz Stars, 2020-07
    94/100 stars
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    92
    Thermo Fisher fugene hd reagent
    DNA transfection reagent enhances internalization of multiple <t>BoNT</t> Lc serotypes in two neuroblastoma cell lines. M17 or N2A cells were exposed for 24 hrs to 50, 10 or 2 nM of BoNT/E toxin (A) or 30 or 6 nM of GST-Lc/E (B) or 50 or 10 nM of BoNT/B toxin (C) or 150 or 30 nM of recombinant Lc/B (D), + or − pre-incubation with <t>FuGene-HD.</t> Cell extracts were prepared and the extent of BoNT intoxication was assessed by Western blotting to monitor SNAP25 or VAMP2 cleavage.
    Fugene Hd Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugene hd reagent/product/Thermo Fisher
    Average 92 stars, based on 44 article reviews
    Price from $9.99 to $1999.99
    fugene hd reagent - by Bioz Stars, 2020-07
    92/100 stars
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    92
    GE Healthcare fugene hd reagent
    DNA transfection reagent enhances internalization of multiple <t>BoNT</t> Lc serotypes in two neuroblastoma cell lines. M17 or N2A cells were exposed for 24 hrs to 50, 10 or 2 nM of BoNT/E toxin (A) or 30 or 6 nM of GST-Lc/E (B) or 50 or 10 nM of BoNT/B toxin (C) or 150 or 30 nM of recombinant Lc/B (D), + or − pre-incubation with <t>FuGene-HD.</t> Cell extracts were prepared and the extent of BoNT intoxication was assessed by Western blotting to monitor SNAP25 or VAMP2 cleavage.
    Fugene Hd Reagent, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugene hd reagent/product/GE Healthcare
    Average 92 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    fugene hd reagent - by Bioz Stars, 2020-07
    92/100 stars
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    Image Search Results


    DNA transfection reagent enhances internalization of multiple BoNT Lc serotypes in two neuroblastoma cell lines. M17 or N2A cells were exposed for 24 hrs to 50, 10 or 2 nM of BoNT/E toxin (A) or 30 or 6 nM of GST-Lc/E (B) or 50 or 10 nM of BoNT/B toxin (C) or 150 or 30 nM of recombinant Lc/B (D), + or − pre-incubation with FuGene-HD. Cell extracts were prepared and the extent of BoNT intoxication was assessed by Western blotting to monitor SNAP25 or VAMP2 cleavage.

    Journal: Toxicon : official journal of the International Society on Toxinology

    Article Title: Lipid and cationic polymer based transduction of botulinum holotoxin, or toxin protease alone, extends the target cell range and improves the efficiency of intoxication

    doi: 10.1016/j.toxicon.2009.10.019

    Figure Lengend Snippet: DNA transfection reagent enhances internalization of multiple BoNT Lc serotypes in two neuroblastoma cell lines. M17 or N2A cells were exposed for 24 hrs to 50, 10 or 2 nM of BoNT/E toxin (A) or 30 or 6 nM of GST-Lc/E (B) or 50 or 10 nM of BoNT/B toxin (C) or 150 or 30 nM of recombinant Lc/B (D), + or − pre-incubation with FuGene-HD. Cell extracts were prepared and the extent of BoNT intoxication was assessed by Western blotting to monitor SNAP25 or VAMP2 cleavage.

    Article Snippet: We observed that neuronal cells intoxicated with BoNT/A immediately after DNA transfection using the FuGene-HD reagent (Roche) appeared more efficiently intoxicated than control cells so we directly tested the effect of FuGene-HD on BoNT intoxication.

    Techniques: Transfection, Recombinant, Incubation, Western Blot

    DNA transfection reagent facilitates rapid BoNT/A internalization by N2A cells and the extent of intoxication is toxin concentration dependent. A. N2A cells were exposed to 10 nM BoNT/A toxin for 0.5, 1, 2, 3, 6 or 24 hrs with (+) or without (−) pre-incubation of toxin with FuGene-HD transfection reagent and immediately harvested. B. BoNT/A toxin was pre-incubated with FuGene-HD then added to N2A cells at 10 nM for 0.5, 1, 2, 3, 6 or 24 hrs. Medium was changed and all the cells were cultured until 48 hrs after initial toxin exposure. C. N2A cells were exposed to 10 nM, 1 nM and 0.1 nM of BoNT/A toxin for 24 hrs with (+) or without (−) pre-incubation with FuGene-HD transfection reagent and then harvested. D. N2A cells were exposed to 10 nM, 1 nM and 0.1 nM of BoNT/A toxin for 3 hrs in the presence of FuGene-HD transfection reagent and then harvested. Cell extracts were prepared and resolved by SDS-PAGE. The extent of BoNT/A intoxication was measured by Western blotting to assess the extent of SNAP25 cleavage.

    Journal: Toxicon : official journal of the International Society on Toxinology

    Article Title: Lipid and cationic polymer based transduction of botulinum holotoxin, or toxin protease alone, extends the target cell range and improves the efficiency of intoxication

    doi: 10.1016/j.toxicon.2009.10.019

    Figure Lengend Snippet: DNA transfection reagent facilitates rapid BoNT/A internalization by N2A cells and the extent of intoxication is toxin concentration dependent. A. N2A cells were exposed to 10 nM BoNT/A toxin for 0.5, 1, 2, 3, 6 or 24 hrs with (+) or without (−) pre-incubation of toxin with FuGene-HD transfection reagent and immediately harvested. B. BoNT/A toxin was pre-incubated with FuGene-HD then added to N2A cells at 10 nM for 0.5, 1, 2, 3, 6 or 24 hrs. Medium was changed and all the cells were cultured until 48 hrs after initial toxin exposure. C. N2A cells were exposed to 10 nM, 1 nM and 0.1 nM of BoNT/A toxin for 24 hrs with (+) or without (−) pre-incubation with FuGene-HD transfection reagent and then harvested. D. N2A cells were exposed to 10 nM, 1 nM and 0.1 nM of BoNT/A toxin for 3 hrs in the presence of FuGene-HD transfection reagent and then harvested. Cell extracts were prepared and resolved by SDS-PAGE. The extent of BoNT/A intoxication was measured by Western blotting to assess the extent of SNAP25 cleavage.

    Article Snippet: We observed that neuronal cells intoxicated with BoNT/A immediately after DNA transfection using the FuGene-HD reagent (Roche) appeared more efficiently intoxicated than control cells so we directly tested the effect of FuGene-HD on BoNT intoxication.

    Techniques: Transfection, Concentration Assay, Incubation, Cell Culture, SDS Page, Western Blot

    DNA transfection reagent facilitates BoNT/A internalization of non-neuronal cell lines. Neuronal cell lines N2A, M17 and non-neuronal cell lines HEK293 (293), HIT-T15 were exposed to 10 nM BoNT/A for 24 hrs with (+) or without (−) pre-incubation with DNA transfection reagents, FuGene-HD or Lipofectamine 2000. Cell extracts were prepared and the extent of BoNT/A intoxication was measured by Western blotting to monitor SNAP25 cleavage.

    Journal: Toxicon : official journal of the International Society on Toxinology

    Article Title: Lipid and cationic polymer based transduction of botulinum holotoxin, or toxin protease alone, extends the target cell range and improves the efficiency of intoxication

    doi: 10.1016/j.toxicon.2009.10.019

    Figure Lengend Snippet: DNA transfection reagent facilitates BoNT/A internalization of non-neuronal cell lines. Neuronal cell lines N2A, M17 and non-neuronal cell lines HEK293 (293), HIT-T15 were exposed to 10 nM BoNT/A for 24 hrs with (+) or without (−) pre-incubation with DNA transfection reagents, FuGene-HD or Lipofectamine 2000. Cell extracts were prepared and the extent of BoNT/A intoxication was measured by Western blotting to monitor SNAP25 cleavage.

    Article Snippet: We observed that neuronal cells intoxicated with BoNT/A immediately after DNA transfection using the FuGene-HD reagent (Roche) appeared more efficiently intoxicated than control cells so we directly tested the effect of FuGene-HD on BoNT intoxication.

    Techniques: Transfection, Incubation, Western Blot

    Lipid-based DNA transfection reagents promote cellular internalization of BoNT/A Lc protease in the absence of Hc. Neuronal cell lines N2A, M17 and non-neuronal cell lines HIT-T15, HEK293 (293) were exposed for 24 hrs to 30 nM of recombinant BoNT/A LC protease, using either the full-length protease (Lc438) or the carboxyl-truncated form (Lc424). The protease was added to cell culture with (+) or without (−) pre-incubation with the DNA transfection reagents, FuGene-HD or Lipofectamine 2000. Cell extracts were prepared and the extent of BoNT/A intoxication was measured by Western blotting to monitor SNAP25 cleavage.

    Journal: Toxicon : official journal of the International Society on Toxinology

    Article Title: Lipid and cationic polymer based transduction of botulinum holotoxin, or toxin protease alone, extends the target cell range and improves the efficiency of intoxication

    doi: 10.1016/j.toxicon.2009.10.019

    Figure Lengend Snippet: Lipid-based DNA transfection reagents promote cellular internalization of BoNT/A Lc protease in the absence of Hc. Neuronal cell lines N2A, M17 and non-neuronal cell lines HIT-T15, HEK293 (293) were exposed for 24 hrs to 30 nM of recombinant BoNT/A LC protease, using either the full-length protease (Lc438) or the carboxyl-truncated form (Lc424). The protease was added to cell culture with (+) or without (−) pre-incubation with the DNA transfection reagents, FuGene-HD or Lipofectamine 2000. Cell extracts were prepared and the extent of BoNT/A intoxication was measured by Western blotting to monitor SNAP25 cleavage.

    Article Snippet: We observed that neuronal cells intoxicated with BoNT/A immediately after DNA transfection using the FuGene-HD reagent (Roche) appeared more efficiently intoxicated than control cells so we directly tested the effect of FuGene-HD on BoNT intoxication.

    Techniques: Transfection, Recombinant, Cell Culture, Incubation, Western Blot

    Defined cationic polymer DNA transfection reagents promote BoNT intoxication independent of DNA transfection. A. N2A cells were incubated for 24 hrs with 20 nM recombinant BoNT/A LC (Lc438) and 0.5 µg pcDNA/CFP expression plasmid with (+) or without (−) pre-incubation with FuGene-HD (FuGene) or PEI having different average molecular weights as indicated. Pre-incubations were performed at a toxin (µg): reagent (µl) ratio of 1:1 with PEI and 1:3 with FuGEne HD. B. N2A cells were incubated for 24 hrs with 20 nM recombinant BoNT/A Lc438 (10 nM for ratios 1:3 and 1:4) and 0.25µg pcDNA/CFP following pre-incubation with FuGene-HD (FuGene) or PEI. (25000 or 75000 MW) at the indicated toxin (µg): reagent (µl) ratio. Cell extracts were prepared and the extent of BoNT/A intoxication was assessed by Western blotting to monitor SNAP25 cleavage. The efficiency of DNA transfection was assayed by Western blotting for CFP.

    Journal: Toxicon : official journal of the International Society on Toxinology

    Article Title: Lipid and cationic polymer based transduction of botulinum holotoxin, or toxin protease alone, extends the target cell range and improves the efficiency of intoxication

    doi: 10.1016/j.toxicon.2009.10.019

    Figure Lengend Snippet: Defined cationic polymer DNA transfection reagents promote BoNT intoxication independent of DNA transfection. A. N2A cells were incubated for 24 hrs with 20 nM recombinant BoNT/A LC (Lc438) and 0.5 µg pcDNA/CFP expression plasmid with (+) or without (−) pre-incubation with FuGene-HD (FuGene) or PEI having different average molecular weights as indicated. Pre-incubations were performed at a toxin (µg): reagent (µl) ratio of 1:1 with PEI and 1:3 with FuGEne HD. B. N2A cells were incubated for 24 hrs with 20 nM recombinant BoNT/A Lc438 (10 nM for ratios 1:3 and 1:4) and 0.25µg pcDNA/CFP following pre-incubation with FuGene-HD (FuGene) or PEI. (25000 or 75000 MW) at the indicated toxin (µg): reagent (µl) ratio. Cell extracts were prepared and the extent of BoNT/A intoxication was assessed by Western blotting to monitor SNAP25 cleavage. The efficiency of DNA transfection was assayed by Western blotting for CFP.

    Article Snippet: We observed that neuronal cells intoxicated with BoNT/A immediately after DNA transfection using the FuGene-HD reagent (Roche) appeared more efficiently intoxicated than control cells so we directly tested the effect of FuGene-HD on BoNT intoxication.

    Techniques: Transfection, Incubation, Recombinant, Expressing, Plasmid Preparation, Western Blot

    Bafilomycin, methylamine hydrochloride and ammonium chloride inhibit DNA transfection reagent-mediated enhancement of BoNT/A holotoxin and Lc internalization into cells. Primary RCGNs, neuronal cell lines N2A, M17 and non-neuronal cell lines 293HEK, HIT-T15 were treated with bafilomycin for 2 hrs and washed with DPBS. Cells were then exposed to 10 nM of BoNT/A toxin for 4 or 24 hrs or 30 nM of Lc438 for 24 hrs, + or − pre-incubation with the FuGene-HD transfection reagent. Control cells were incubated with 10 nM of BoNT/A toxin or 30 nM of Lc438, + or − pre-incubation with FuGene-HD, without prior exposure of the cells to bafilomycin. Cell extracts were prepared and the extent of BoNT/A intoxication was assessed by Western blotting to monitor SNAP25 cleavage. B. N2A, M17 and HIT-T15 cells were treated +/− bafilomycin and then transfected 24 hrs with pcDNA/CFP in FuGene-HD as above. Cell extracts were resolved by SDS-PAGE and CFP was detected by Western blotting. C. M17 cells were treated with 10 mM methylamine hydrochloride for 1 h or 8 mM ammonium chloride (NH 4 Cl) for 2 hrs and then exposed to 10 nM Lc438 with (+) or without (−) FuGene-HD transfection reagent (1:3) for 4 hrs. Cell extracts were prepared and the extent of BoNT/A intoxication was assessed by Western blotting to monitor SNAP25 cleavage.

    Journal: Toxicon : official journal of the International Society on Toxinology

    Article Title: Lipid and cationic polymer based transduction of botulinum holotoxin, or toxin protease alone, extends the target cell range and improves the efficiency of intoxication

    doi: 10.1016/j.toxicon.2009.10.019

    Figure Lengend Snippet: Bafilomycin, methylamine hydrochloride and ammonium chloride inhibit DNA transfection reagent-mediated enhancement of BoNT/A holotoxin and Lc internalization into cells. Primary RCGNs, neuronal cell lines N2A, M17 and non-neuronal cell lines 293HEK, HIT-T15 were treated with bafilomycin for 2 hrs and washed with DPBS. Cells were then exposed to 10 nM of BoNT/A toxin for 4 or 24 hrs or 30 nM of Lc438 for 24 hrs, + or − pre-incubation with the FuGene-HD transfection reagent. Control cells were incubated with 10 nM of BoNT/A toxin or 30 nM of Lc438, + or − pre-incubation with FuGene-HD, without prior exposure of the cells to bafilomycin. Cell extracts were prepared and the extent of BoNT/A intoxication was assessed by Western blotting to monitor SNAP25 cleavage. B. N2A, M17 and HIT-T15 cells were treated +/− bafilomycin and then transfected 24 hrs with pcDNA/CFP in FuGene-HD as above. Cell extracts were resolved by SDS-PAGE and CFP was detected by Western blotting. C. M17 cells were treated with 10 mM methylamine hydrochloride for 1 h or 8 mM ammonium chloride (NH 4 Cl) for 2 hrs and then exposed to 10 nM Lc438 with (+) or without (−) FuGene-HD transfection reagent (1:3) for 4 hrs. Cell extracts were prepared and the extent of BoNT/A intoxication was assessed by Western blotting to monitor SNAP25 cleavage.

    Article Snippet: We observed that neuronal cells intoxicated with BoNT/A immediately after DNA transfection using the FuGene-HD reagent (Roche) appeared more efficiently intoxicated than control cells so we directly tested the effect of FuGene-HD on BoNT intoxication.

    Techniques: Transfection, Incubation, Western Blot, SDS Page