fugene Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Boehringer Mannheim fugene 6
    Glomerular H-E staining scores in the kidney samples on day 4 (4-d) and weeks 3 (3-w) and 5 (5-w) after repeated injections of <t>pCAG/DT-A/FuGENE™6</t> complex, pCAG/FuGENE™6 complex or mock injections [FuGENE™6 only or PBS(-) only]. At least 100 glomeruli in the H-E-stained sections were randomly chosen per kidney and the lesions were quantified. A total of 4 kidneys for each group were inspected. The glomerular lesions were graded from 0 to 2: score 0, normal; score 1, weak to mild change (exhibiting mild mesangial cell proliferation and mesangial matrix expansion); score 2, severe change (exhibiting lobular structure of glomeruli and adhesion of glomeruli to the inner surface of Bowman's capsule).
    Fugene 6, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 955 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugene 6/product/Boehringer Mannheim
    Average 92 stars, based on 955 article reviews
    Price from $9.99 to $1999.99
    fugene 6 - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    94
    Roche fugene 6
    Effect of cytofectins on CT DNA uptake by macrophages. J774 cells were incubated for up to 48 hr with 0·4–10 µg/ml EMA-CT DNA with or without Fugene 6. DNA uptake was assessed by flow cytometric analysis of the cells collected at different time-points. The levels of DNA uptake are represented as mean fluorescence intensity (MFI) for each group. There was a dose- and time-dependent increase of DNA uptake in the presence of Fugene 6.
    Fugene 6, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 41961 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugene 6/product/Roche
    Average 94 stars, based on 41961 article reviews
    Price from $9.99 to $1999.99
    fugene 6 - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    92
    Thermo Fisher fugene
    Effect of cytofectins on CT DNA uptake by macrophages. J774 cells were incubated for up to 48 hr with 0·4–10 µg/ml EMA-CT DNA with or without Fugene 6. DNA uptake was assessed by flow cytometric analysis of the cells collected at different time-points. The levels of DNA uptake are represented as mean fluorescence intensity (MFI) for each group. There was a dose- and time-dependent increase of DNA uptake in the presence of Fugene 6.
    Fugene, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 395 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugene/product/Thermo Fisher
    Average 92 stars, based on 395 article reviews
    Price from $9.99 to $1999.99
    fugene - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    90
    Boehringer Ingelheim fugene 6
    Effect of cytofectins on CT DNA uptake by macrophages. J774 cells were incubated for up to 48 hr with 0·4–10 µg/ml EMA-CT DNA with or without Fugene 6. DNA uptake was assessed by flow cytometric analysis of the cells collected at different time-points. The levels of DNA uptake are represented as mean fluorescence intensity (MFI) for each group. There was a dose- and time-dependent increase of DNA uptake in the presence of Fugene 6.
    Fugene 6, supplied by Boehringer Ingelheim, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugene 6/product/Boehringer Ingelheim
    Average 90 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    fugene 6 - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    92
    GE Healthcare fugene 6
    Effect of cytofectins on CT DNA uptake by macrophages. J774 cells were incubated for up to 48 hr with 0·4–10 µg/ml EMA-CT DNA with or without Fugene 6. DNA uptake was assessed by flow cytometric analysis of the cells collected at different time-points. The levels of DNA uptake are represented as mean fluorescence intensity (MFI) for each group. There was a dose- and time-dependent increase of DNA uptake in the presence of Fugene 6.
    Fugene 6, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugene 6/product/GE Healthcare
    Average 92 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    fugene 6 - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    Mirus Bio fugene
    Effect of cytofectins on CT DNA uptake by macrophages. J774 cells were incubated for up to 48 hr with 0·4–10 µg/ml EMA-CT DNA with or without Fugene 6. DNA uptake was assessed by flow cytometric analysis of the cells collected at different time-points. The levels of DNA uptake are represented as mean fluorescence intensity (MFI) for each group. There was a dose- and time-dependent increase of DNA uptake in the presence of Fugene 6.
    Fugene, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugene/product/Mirus Bio
    Average 92 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    fugene - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    91
    Stratagene fugene 6
    Effect of cytofectins on CT DNA uptake by macrophages. J774 cells were incubated for up to 48 hr with 0·4–10 µg/ml EMA-CT DNA with or without Fugene 6. DNA uptake was assessed by flow cytometric analysis of the cells collected at different time-points. The levels of DNA uptake are represented as mean fluorescence intensity (MFI) for each group. There was a dose- and time-dependent increase of DNA uptake in the presence of Fugene 6.
    Fugene 6, supplied by Stratagene, used in various techniques. Bioz Stars score: 91/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugene 6/product/Stratagene
    Average 91 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    fugene 6 - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    92
    SwitchGear Genomics fugene
    Effect of cytofectins on CT DNA uptake by macrophages. J774 cells were incubated for up to 48 hr with 0·4–10 µg/ml EMA-CT DNA with or without Fugene 6. DNA uptake was assessed by flow cytometric analysis of the cells collected at different time-points. The levels of DNA uptake are represented as mean fluorescence intensity (MFI) for each group. There was a dose- and time-dependent increase of DNA uptake in the presence of Fugene 6.
    Fugene, supplied by SwitchGear Genomics, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugene/product/SwitchGear Genomics
    Average 92 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    fugene - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    99
    Promega fugene 6
    Z potential of control microparticles and of microparticles treated with PEI, LF2000 and <t>FuGENE</t> 6 ® . Note: The Zeta potential of the functionalized microparticle is also shown. Abbreviations: FMP, functionalized microparticle; PEI, polyethyleneimine; LF, Lipofectamine™.
    Fugene 6, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 10068 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugene 6/product/Promega
    Average 99 stars, based on 10068 article reviews
    Price from $9.99 to $1999.99
    fugene 6 - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    85
    Roche fugene g
    Z potential of control microparticles and of microparticles treated with PEI, LF2000 and <t>FuGENE</t> 6 ® . Note: The Zeta potential of the functionalized microparticle is also shown. Abbreviations: FMP, functionalized microparticle; PEI, polyethyleneimine; LF, Lipofectamine™.
    Fugene G, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugene g/product/Roche
    Average 85 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    fugene g - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    99
    Promega fugene hd
    Transfection. Representative phase contrast and fluorescence images (10x) of <t>ASSCs</t> transfected with a GFP expression plasmid by different methods (Electroporation, LTX, PEI and <t>Fugene</t> HD), along with the efficiency of transfection as measured by cytometry. Each set of transfections was performed three times and standard deviations were negligible.
    Fugene Hd, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 15329 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugene hd/product/Promega
    Average 99 stars, based on 15329 article reviews
    Price from $9.99 to $1999.99
    fugene hd - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    95
    Roche fugene hd
    Silencing in GFP-expressing THP-1 cells. (A) The cell counts as measured by flow cytometry (expressed as a percentage of non-treated cells). ( B ) Silencing was assessed after 3 days of siRNA treatment (50 nM) and expressed as decrease in mean GFP fluorescence or decrease in GFP-positive cells. The polymer:siRNA ratios were 2∶1, 4∶1 and 8∶1. ( C ) Dose-response curves for GFP silencing between 25 and 200 nM siRNA treatment. The CA and LA substituted polymers were used at the polymer:siRNA ratios of 4∶1 and 8∶1, and silencing was assessed after 3 days of treatment. ( D ) Silencing by CA- and LA-substituted polymers and three commercial reagents (Lipofectamine™ 2000, Metafectamine and <t>Fugene</t> HD). The extent of silencing was summarized over a course of 9 days and expressed as decrease in mean GFP fluorescence (top panel) or decrease in GFP-positive cells (middle panel). The lipid substitutions of the polymers used were 2.1 LA/PEI (PEI2-LA) and 6.9 CA/PEI (PEI2-CA).
    Fugene Hd, supplied by Roche, used in various techniques. Bioz Stars score: 95/100, based on 15476 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugene hd/product/Roche
    Average 95 stars, based on 15476 article reviews
    Price from $9.99 to $1999.99
    fugene hd - by Bioz Stars, 2020-08
    95/100 stars
      Buy from Supplier

    90
    SwitchGear Genomics fugene hd
    Silencing in GFP-expressing THP-1 cells. (A) The cell counts as measured by flow cytometry (expressed as a percentage of non-treated cells). ( B ) Silencing was assessed after 3 days of siRNA treatment (50 nM) and expressed as decrease in mean GFP fluorescence or decrease in GFP-positive cells. The polymer:siRNA ratios were 2∶1, 4∶1 and 8∶1. ( C ) Dose-response curves for GFP silencing between 25 and 200 nM siRNA treatment. The CA and LA substituted polymers were used at the polymer:siRNA ratios of 4∶1 and 8∶1, and silencing was assessed after 3 days of treatment. ( D ) Silencing by CA- and LA-substituted polymers and three commercial reagents (Lipofectamine™ 2000, Metafectamine and <t>Fugene</t> HD). The extent of silencing was summarized over a course of 9 days and expressed as decrease in mean GFP fluorescence (top panel) or decrease in GFP-positive cells (middle panel). The lipid substitutions of the polymers used were 2.1 LA/PEI (PEI2-LA) and 6.9 CA/PEI (PEI2-CA).
    Fugene Hd, supplied by SwitchGear Genomics, used in various techniques. Bioz Stars score: 90/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugene hd/product/SwitchGear Genomics
    Average 90 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    fugene hd - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    93
    Thermo Fisher fugene hd
    Silencing in GFP-expressing THP-1 cells. (A) The cell counts as measured by flow cytometry (expressed as a percentage of non-treated cells). ( B ) Silencing was assessed after 3 days of siRNA treatment (50 nM) and expressed as decrease in mean GFP fluorescence or decrease in GFP-positive cells. The polymer:siRNA ratios were 2∶1, 4∶1 and 8∶1. ( C ) Dose-response curves for GFP silencing between 25 and 200 nM siRNA treatment. The CA and LA substituted polymers were used at the polymer:siRNA ratios of 4∶1 and 8∶1, and silencing was assessed after 3 days of treatment. ( D ) Silencing by CA- and LA-substituted polymers and three commercial reagents (Lipofectamine™ 2000, Metafectamine and <t>Fugene</t> HD). The extent of silencing was summarized over a course of 9 days and expressed as decrease in mean GFP fluorescence (top panel) or decrease in GFP-positive cells (middle panel). The lipid substitutions of the polymers used were 2.1 LA/PEI (PEI2-LA) and 6.9 CA/PEI (PEI2-CA).
    Fugene Hd, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 579 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugene hd/product/Thermo Fisher
    Average 93 stars, based on 579 article reviews
    Price from $9.99 to $1999.99
    fugene hd - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    85
    Roche fugene hdtm
    Silencing in GFP-expressing THP-1 cells. (A) The cell counts as measured by flow cytometry (expressed as a percentage of non-treated cells). ( B ) Silencing was assessed after 3 days of siRNA treatment (50 nM) and expressed as decrease in mean GFP fluorescence or decrease in GFP-positive cells. The polymer:siRNA ratios were 2∶1, 4∶1 and 8∶1. ( C ) Dose-response curves for GFP silencing between 25 and 200 nM siRNA treatment. The CA and LA substituted polymers were used at the polymer:siRNA ratios of 4∶1 and 8∶1, and silencing was assessed after 3 days of treatment. ( D ) Silencing by CA- and LA-substituted polymers and three commercial reagents (Lipofectamine™ 2000, Metafectamine and <t>Fugene</t> HD). The extent of silencing was summarized over a course of 9 days and expressed as decrease in mean GFP fluorescence (top panel) or decrease in GFP-positive cells (middle panel). The lipid substitutions of the polymers used were 2.1 LA/PEI (PEI2-LA) and 6.9 CA/PEI (PEI2-CA).
    Fugene Hdtm, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugene hdtm/product/Roche
    Average 85 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    fugene hdtm - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    91
    Promega fugene hf
    Silencing in GFP-expressing THP-1 cells. (A) The cell counts as measured by flow cytometry (expressed as a percentage of non-treated cells). ( B ) Silencing was assessed after 3 days of siRNA treatment (50 nM) and expressed as decrease in mean GFP fluorescence or decrease in GFP-positive cells. The polymer:siRNA ratios were 2∶1, 4∶1 and 8∶1. ( C ) Dose-response curves for GFP silencing between 25 and 200 nM siRNA treatment. The CA and LA substituted polymers were used at the polymer:siRNA ratios of 4∶1 and 8∶1, and silencing was assessed after 3 days of treatment. ( D ) Silencing by CA- and LA-substituted polymers and three commercial reagents (Lipofectamine™ 2000, Metafectamine and <t>Fugene</t> HD). The extent of silencing was summarized over a course of 9 days and expressed as decrease in mean GFP fluorescence (top panel) or decrease in GFP-positive cells (middle panel). The lipid substitutions of the polymers used were 2.1 LA/PEI (PEI2-LA) and 6.9 CA/PEI (PEI2-CA).
    Fugene Hf, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugene hf/product/Promega
    Average 91 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    fugene hf - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    85
    Roche fugene hg
    Silencing in GFP-expressing THP-1 cells. (A) The cell counts as measured by flow cytometry (expressed as a percentage of non-treated cells). ( B ) Silencing was assessed after 3 days of siRNA treatment (50 nM) and expressed as decrease in mean GFP fluorescence or decrease in GFP-positive cells. The polymer:siRNA ratios were 2∶1, 4∶1 and 8∶1. ( C ) Dose-response curves for GFP silencing between 25 and 200 nM siRNA treatment. The CA and LA substituted polymers were used at the polymer:siRNA ratios of 4∶1 and 8∶1, and silencing was assessed after 3 days of treatment. ( D ) Silencing by CA- and LA-substituted polymers and three commercial reagents (Lipofectamine™ 2000, Metafectamine and <t>Fugene</t> HD). The extent of silencing was summarized over a course of 9 days and expressed as decrease in mean GFP fluorescence (top panel) or decrease in GFP-positive cells (middle panel). The lipid substitutions of the polymers used were 2.1 LA/PEI (PEI2-LA) and 6.9 CA/PEI (PEI2-CA).
    Fugene Hg, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugene hg/product/Roche
    Average 85 stars, based on 37 article reviews
    Price from $9.99 to $1999.99
    fugene hg - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    88
    Promega fugene hp
    Silencing in GFP-expressing THP-1 cells. (A) The cell counts as measured by flow cytometry (expressed as a percentage of non-treated cells). ( B ) Silencing was assessed after 3 days of siRNA treatment (50 nM) and expressed as decrease in mean GFP fluorescence or decrease in GFP-positive cells. The polymer:siRNA ratios were 2∶1, 4∶1 and 8∶1. ( C ) Dose-response curves for GFP silencing between 25 and 200 nM siRNA treatment. The CA and LA substituted polymers were used at the polymer:siRNA ratios of 4∶1 and 8∶1, and silencing was assessed after 3 days of treatment. ( D ) Silencing by CA- and LA-substituted polymers and three commercial reagents (Lipofectamine™ 2000, Metafectamine and <t>Fugene</t> HD). The extent of silencing was summarized over a course of 9 days and expressed as decrease in mean GFP fluorescence (top panel) or decrease in GFP-positive cells (middle panel). The lipid substitutions of the polymers used were 2.1 LA/PEI (PEI2-LA) and 6.9 CA/PEI (PEI2-CA).
    Fugene Hp, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugene hp/product/Promega
    Average 88 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    fugene hp - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    88
    Roche fugene kit
    Silencing in GFP-expressing THP-1 cells. (A) The cell counts as measured by flow cytometry (expressed as a percentage of non-treated cells). ( B ) Silencing was assessed after 3 days of siRNA treatment (50 nM) and expressed as decrease in mean GFP fluorescence or decrease in GFP-positive cells. The polymer:siRNA ratios were 2∶1, 4∶1 and 8∶1. ( C ) Dose-response curves for GFP silencing between 25 and 200 nM siRNA treatment. The CA and LA substituted polymers were used at the polymer:siRNA ratios of 4∶1 and 8∶1, and silencing was assessed after 3 days of treatment. ( D ) Silencing by CA- and LA-substituted polymers and three commercial reagents (Lipofectamine™ 2000, Metafectamine and <t>Fugene</t> HD). The extent of silencing was summarized over a course of 9 days and expressed as decrease in mean GFP fluorescence (top panel) or decrease in GFP-positive cells (middle panel). The lipid substitutions of the polymers used were 2.1 LA/PEI (PEI2-LA) and 6.9 CA/PEI (PEI2-CA).
    Fugene Kit, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugene kit/product/Roche
    Average 88 stars, based on 51 article reviews
    Price from $9.99 to $1999.99
    fugene kit - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    92
    Boehringer Mannheim fugene reagent
    Silencing in GFP-expressing THP-1 cells. (A) The cell counts as measured by flow cytometry (expressed as a percentage of non-treated cells). ( B ) Silencing was assessed after 3 days of siRNA treatment (50 nM) and expressed as decrease in mean GFP fluorescence or decrease in GFP-positive cells. The polymer:siRNA ratios were 2∶1, 4∶1 and 8∶1. ( C ) Dose-response curves for GFP silencing between 25 and 200 nM siRNA treatment. The CA and LA substituted polymers were used at the polymer:siRNA ratios of 4∶1 and 8∶1, and silencing was assessed after 3 days of treatment. ( D ) Silencing by CA- and LA-substituted polymers and three commercial reagents (Lipofectamine™ 2000, Metafectamine and <t>Fugene</t> HD). The extent of silencing was summarized over a course of 9 days and expressed as decrease in mean GFP fluorescence (top panel) or decrease in GFP-positive cells (middle panel). The lipid substitutions of the polymers used were 2.1 LA/PEI (PEI2-LA) and 6.9 CA/PEI (PEI2-CA).
    Fugene Reagent, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugene reagent/product/Boehringer Mannheim
    Average 92 stars, based on 89 article reviews
    Price from $9.99 to $1999.99
    fugene reagent - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    94
    Roche fugene 6 reagent
    Activity of the pTα enhancer in various T cell lines. The 4.3- and 0.35-kb pTα enhancer fragments and the control CD3δ enhancer were subcloned upstream of the SV40 promoter/LacZ reporter gene. (A) The cells were transfected using <t>Fugene</t> 6 reagent. A CMV promoter/LacZ construct was used as a positive control for transfection of BW5147 cells. In B, the cells were transfected using Superfect reagent.
    Fugene 6 Reagent, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 7417 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugene 6 reagent/product/Roche
    Average 94 stars, based on 7417 article reviews
    Price from $9.99 to $1999.99
    fugene 6 reagent - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    88
    Roche fugene vi
    Activity of the pTα enhancer in various T cell lines. The 4.3- and 0.35-kb pTα enhancer fragments and the control CD3δ enhancer were subcloned upstream of the SV40 promoter/LacZ reporter gene. (A) The cells were transfected using <t>Fugene</t> 6 reagent. A CMV promoter/LacZ construct was used as a positive control for transfection of BW5147 cells. In B, the cells were transfected using Superfect reagent.
    Fugene Vi, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugene vi/product/Roche
    Average 88 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    fugene vi - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    92
    Fisher Scientific fugene 6
    Activity of the pTα enhancer in various T cell lines. The 4.3- and 0.35-kb pTα enhancer fragments and the control CD3δ enhancer were subcloned upstream of the SV40 promoter/LacZ reporter gene. (A) The cells were transfected using <t>Fugene</t> 6 reagent. A CMV promoter/LacZ construct was used as a positive control for transfection of BW5147 cells. In B, the cells were transfected using Superfect reagent.
    Fugene 6, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugene 6/product/Fisher Scientific
    Average 92 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    fugene 6 - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    88
    Roche fugene 6tm
    Activity of the pTα enhancer in various T cell lines. The 4.3- and 0.35-kb pTα enhancer fragments and the control CD3δ enhancer were subcloned upstream of the SV40 promoter/LacZ reporter gene. (A) The cells were transfected using <t>Fugene</t> 6 reagent. A CMV promoter/LacZ construct was used as a positive control for transfection of BW5147 cells. In B, the cells were transfected using Superfect reagent.
    Fugene 6tm, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugene 6tm/product/Roche
    Average 88 stars, based on 110 article reviews
    Price from $9.99 to $1999.99
    fugene 6tm - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    Image Search Results


    Glomerular H-E staining scores in the kidney samples on day 4 (4-d) and weeks 3 (3-w) and 5 (5-w) after repeated injections of pCAG/DT-A/FuGENE™6 complex, pCAG/FuGENE™6 complex or mock injections [FuGENE™6 only or PBS(-) only]. At least 100 glomeruli in the H-E-stained sections were randomly chosen per kidney and the lesions were quantified. A total of 4 kidneys for each group were inspected. The glomerular lesions were graded from 0 to 2: score 0, normal; score 1, weak to mild change (exhibiting mild mesangial cell proliferation and mesangial matrix expansion); score 2, severe change (exhibiting lobular structure of glomeruli and adhesion of glomeruli to the inner surface of Bowman's capsule).

    Journal: BMC Nephrology

    Article Title: A new mouse model for renal lesions produced by intravenous injection of diphtheria toxin A-chain expression plasmid

    doi: 10.1186/1471-2369-5-4

    Figure Lengend Snippet: Glomerular H-E staining scores in the kidney samples on day 4 (4-d) and weeks 3 (3-w) and 5 (5-w) after repeated injections of pCAG/DT-A/FuGENE™6 complex, pCAG/FuGENE™6 complex or mock injections [FuGENE™6 only or PBS(-) only]. At least 100 glomeruli in the H-E-stained sections were randomly chosen per kidney and the lesions were quantified. A total of 4 kidneys for each group were inspected. The glomerular lesions were graded from 0 to 2: score 0, normal; score 1, weak to mild change (exhibiting mild mesangial cell proliferation and mesangial matrix expansion); score 2, severe change (exhibiting lobular structure of glomeruli and adhesion of glomeruli to the inner surface of Bowman's capsule).

    Article Snippet: For control injection (mock injection), a solution containing 40 μl of FuGENE™6 dissolved in PBS(-) (without DNA) or PBS(-) only was prepared.

    Techniques: Staining

    Serum levels of BUN, CRT, Na + , K + and Cl - in the mice repeatedly injected with pCAG/DT-A/FuGENE™6 complex, pCAG/FuGENE™6 complex, FuGENE™6 only or PBS(-) only. Serum sampling was performed on day 4(4-d) and weeks 3 (3-w) and 5 (5-w).

    Journal: BMC Nephrology

    Article Title: A new mouse model for renal lesions produced by intravenous injection of diphtheria toxin A-chain expression plasmid

    doi: 10.1186/1471-2369-5-4

    Figure Lengend Snippet: Serum levels of BUN, CRT, Na + , K + and Cl - in the mice repeatedly injected with pCAG/DT-A/FuGENE™6 complex, pCAG/FuGENE™6 complex, FuGENE™6 only or PBS(-) only. Serum sampling was performed on day 4(4-d) and weeks 3 (3-w) and 5 (5-w).

    Article Snippet: For control injection (mock injection), a solution containing 40 μl of FuGENE™6 dissolved in PBS(-) (without DNA) or PBS(-) only was prepared.

    Techniques: Mouse Assay, Injection, Sampling

    ( A ) Detection of exogenous pCAG/DT-A by PCR analysis. Genomic DNA was extracted from organs of the mice injected with pCAG/DT-A/FuGENE™6 complex or PBS(-) (mock injection). Detection of pCAG/DT-A was performed by PCR using DTA-S/DTA-2RV primer set (see Figure 1A ). This PCR yielded a product of 267 bp [indicated by an arrow in ( A )]. C indicates that tail DNA (1 μg) from a B6C3F1 mouse was directly subjected to PCR as a negative control. PC indicates that pCAG/DT-A (4 ng) was subjected to PCR as a positive control. ( B ) Detection of DT-A mRNA by RT-PCR analysis. Detection of DT-A mRNA was performed by RT-PCR using βA-1/DTA-RV primer set (see Figure 1A ), which yielded an expected band of 300 bp [indicated by an arrow in ( B )]. In the lower panel of ( B ), the expression pattern of endogenous β-actin mRNA is indicated. Lu, lung; Br, brain; H, heart; K, kidney; In, intestine; L, liver. C-1 indicates that tail DNA (1 μg) from a B6C3F1 mouse was directly subjected to PCR. C-2 indicates that tail DNA (1 μg) from a B6C3F1 mouse was directly subjected to reverse transcription and the resulting solution was then PCR-amplified. PC indicates that pCAG/DT-A (4 ng) was subjected to RT-PCR as a positive control. A few non-specifically amplified bands above the 300-bp band were observed in lane C-2, and approximately 600-bp bands [indicated by an arrowhead in ( B )], probably corresponding to the products obtained through amplification of pCAG/DT-A, were also seen in lane PC.

    Journal: BMC Nephrology

    Article Title: A new mouse model for renal lesions produced by intravenous injection of diphtheria toxin A-chain expression plasmid

    doi: 10.1186/1471-2369-5-4

    Figure Lengend Snippet: ( A ) Detection of exogenous pCAG/DT-A by PCR analysis. Genomic DNA was extracted from organs of the mice injected with pCAG/DT-A/FuGENE™6 complex or PBS(-) (mock injection). Detection of pCAG/DT-A was performed by PCR using DTA-S/DTA-2RV primer set (see Figure 1A ). This PCR yielded a product of 267 bp [indicated by an arrow in ( A )]. C indicates that tail DNA (1 μg) from a B6C3F1 mouse was directly subjected to PCR as a negative control. PC indicates that pCAG/DT-A (4 ng) was subjected to PCR as a positive control. ( B ) Detection of DT-A mRNA by RT-PCR analysis. Detection of DT-A mRNA was performed by RT-PCR using βA-1/DTA-RV primer set (see Figure 1A ), which yielded an expected band of 300 bp [indicated by an arrow in ( B )]. In the lower panel of ( B ), the expression pattern of endogenous β-actin mRNA is indicated. Lu, lung; Br, brain; H, heart; K, kidney; In, intestine; L, liver. C-1 indicates that tail DNA (1 μg) from a B6C3F1 mouse was directly subjected to PCR. C-2 indicates that tail DNA (1 μg) from a B6C3F1 mouse was directly subjected to reverse transcription and the resulting solution was then PCR-amplified. PC indicates that pCAG/DT-A (4 ng) was subjected to RT-PCR as a positive control. A few non-specifically amplified bands above the 300-bp band were observed in lane C-2, and approximately 600-bp bands [indicated by an arrowhead in ( B )], probably corresponding to the products obtained through amplification of pCAG/DT-A, were also seen in lane PC.

    Article Snippet: For control injection (mock injection), a solution containing 40 μl of FuGENE™6 dissolved in PBS(-) (without DNA) or PBS(-) only was prepared.

    Techniques: Polymerase Chain Reaction, Mouse Assay, Injection, Negative Control, Positive Control, Reverse Transcription Polymerase Chain Reaction, Expressing, Amplification

    EGFP fluorescence in 4% paraformaldehyde-fixed heart (a), lung (b,c) and kidneys (d-f) of mice injected 6 times with pCE-29/ FuGENE™6 complex. (a) Inner surface of the heart cut into slices. Note that the luminal surface of the heart exhibited focal and slight EGFP fluorescence (indicated by arrows). (b,c) Inner surface (b) of the lung cut into slices and outer (c) surface of the lung. Note focal or patchy expression of EGFP fluorescence in each sample (indicated by arrows). (d) Inner surface of the kidney cut into slices. Note that several glomeruli expressed EGFP fluorescence (indicated by arrows). (e) A cryostat section of the kidney in (d). Patchy expression for EGFP fluorescence (indicated by an arrow) in glomeruli was noted. Also, some renal tubules exhibited faint fluorescence (indicated by arrowheads). (f) A figure magnified from the boxed region in (e). EGFP fluorescence was patchy in the glomerulus (indicated by arrows).

    Journal: BMC Nephrology

    Article Title: A new mouse model for renal lesions produced by intravenous injection of diphtheria toxin A-chain expression plasmid

    doi: 10.1186/1471-2369-5-4

    Figure Lengend Snippet: EGFP fluorescence in 4% paraformaldehyde-fixed heart (a), lung (b,c) and kidneys (d-f) of mice injected 6 times with pCE-29/ FuGENE™6 complex. (a) Inner surface of the heart cut into slices. Note that the luminal surface of the heart exhibited focal and slight EGFP fluorescence (indicated by arrows). (b,c) Inner surface (b) of the lung cut into slices and outer (c) surface of the lung. Note focal or patchy expression of EGFP fluorescence in each sample (indicated by arrows). (d) Inner surface of the kidney cut into slices. Note that several glomeruli expressed EGFP fluorescence (indicated by arrows). (e) A cryostat section of the kidney in (d). Patchy expression for EGFP fluorescence (indicated by an arrow) in glomeruli was noted. Also, some renal tubules exhibited faint fluorescence (indicated by arrowheads). (f) A figure magnified from the boxed region in (e). EGFP fluorescence was patchy in the glomerulus (indicated by arrows).

    Article Snippet: For control injection (mock injection), a solution containing 40 μl of FuGENE™6 dissolved in PBS(-) (without DNA) or PBS(-) only was prepared.

    Techniques: Fluorescence, Mouse Assay, Injection, Expressing

    H-E staining of glomeruli and renal tubules in the kidneys of mice injected 6 times with pCAG/DT-A/FuGENE™6 complex (a-i), pCAG/FuGENE™6 complex (j) or PBS(-) only (k) on day 4 (a-g) and weeks 3 (h) and 5 (i). On day 4, proliferation of mesangial cells, which causes loss of Bowman's space [indicated by arrows in (a) and (b)] and attenuation of glomerular capillaries [indicated by arrowheads in (c)] were remarkable. In some cases, findings of cellular degeneration [indicated by arrows in (c) and (e)] and appearance of lobular segmental structure of glomerulus (d-f) were observed. Infiltration of lymphocytes into interstitum and renal tubules [indicated by arrowheads in (g)] was sometimes observed. However, no gross alteration in glomeruli was noted in the groups with injection of pCAG/FuGENE™6 complex (j) and of mock injections with FuGENE™6 only (data not shown) or PBS(-) only (k). On week 3, slight recovery was observed in the mice treated with pCAG/DT-A/FuGENE™6 complex, although debris [indicated by an arrow in (h)] existed in Bowman's space. By week 5, the morphology of glomeruli had almost completely recovered, although proteinaceous substances were often observed in Bowman's space (i). (l), Glomerulus of intact kidney.

    Journal: BMC Nephrology

    Article Title: A new mouse model for renal lesions produced by intravenous injection of diphtheria toxin A-chain expression plasmid

    doi: 10.1186/1471-2369-5-4

    Figure Lengend Snippet: H-E staining of glomeruli and renal tubules in the kidneys of mice injected 6 times with pCAG/DT-A/FuGENE™6 complex (a-i), pCAG/FuGENE™6 complex (j) or PBS(-) only (k) on day 4 (a-g) and weeks 3 (h) and 5 (i). On day 4, proliferation of mesangial cells, which causes loss of Bowman's space [indicated by arrows in (a) and (b)] and attenuation of glomerular capillaries [indicated by arrowheads in (c)] were remarkable. In some cases, findings of cellular degeneration [indicated by arrows in (c) and (e)] and appearance of lobular segmental structure of glomerulus (d-f) were observed. Infiltration of lymphocytes into interstitum and renal tubules [indicated by arrowheads in (g)] was sometimes observed. However, no gross alteration in glomeruli was noted in the groups with injection of pCAG/FuGENE™6 complex (j) and of mock injections with FuGENE™6 only (data not shown) or PBS(-) only (k). On week 3, slight recovery was observed in the mice treated with pCAG/DT-A/FuGENE™6 complex, although debris [indicated by an arrow in (h)] existed in Bowman's space. By week 5, the morphology of glomeruli had almost completely recovered, although proteinaceous substances were often observed in Bowman's space (i). (l), Glomerulus of intact kidney.

    Article Snippet: For control injection (mock injection), a solution containing 40 μl of FuGENE™6 dissolved in PBS(-) (without DNA) or PBS(-) only was prepared.

    Techniques: Staining, Mouse Assay, Injection

    Electron microscopic analysis of affected glomeruli (a, c-f, h), and tubules (g) on day 4 (a, c-g) and week 5 (h) after injection of pCAG/DT-A/FuGENE™6 complex. In the affected glomeruli (a), severe matrix expansion with numerous electron-dense deposits was detected in the mesangial matrix. The capillary lumen of endothelial cells was deformed, probably due to proliferation of active mesangial cells. Degeneration of mesangial cells was sometimes observed [indicated by an arrow in (a)]. In contrast, the control glomeruli derived from mock injection of PBS(-) on day 4 (b) exhibited normal appearance. Notably, formation of focal deposits in glomerular basement membrane was often remarkable [indicated by arrows in (c-f)]. The initial, small deposits of glomerular basement membrane are shown by arrowheads in (d) and (e). Incorporation of lipids, probably DNA/lipid complexes that had been re-uptaken by renal tubules, was noted in the cytoplasm of renal tubules [indicated by arrows in (g)]. On week 5, nearly normal glomerular structure was observed (h).

    Journal: BMC Nephrology

    Article Title: A new mouse model for renal lesions produced by intravenous injection of diphtheria toxin A-chain expression plasmid

    doi: 10.1186/1471-2369-5-4

    Figure Lengend Snippet: Electron microscopic analysis of affected glomeruli (a, c-f, h), and tubules (g) on day 4 (a, c-g) and week 5 (h) after injection of pCAG/DT-A/FuGENE™6 complex. In the affected glomeruli (a), severe matrix expansion with numerous electron-dense deposits was detected in the mesangial matrix. The capillary lumen of endothelial cells was deformed, probably due to proliferation of active mesangial cells. Degeneration of mesangial cells was sometimes observed [indicated by an arrow in (a)]. In contrast, the control glomeruli derived from mock injection of PBS(-) on day 4 (b) exhibited normal appearance. Notably, formation of focal deposits in glomerular basement membrane was often remarkable [indicated by arrows in (c-f)]. The initial, small deposits of glomerular basement membrane are shown by arrowheads in (d) and (e). Incorporation of lipids, probably DNA/lipid complexes that had been re-uptaken by renal tubules, was noted in the cytoplasm of renal tubules [indicated by arrows in (g)]. On week 5, nearly normal glomerular structure was observed (h).

    Article Snippet: For control injection (mock injection), a solution containing 40 μl of FuGENE™6 dissolved in PBS(-) (without DNA) or PBS(-) only was prepared.

    Techniques: Injection, Derivative Assay

    PAS and PAM staining of kidney sections sampled day 4 (d-f) and weeks 3 (g-i) and 5 (j-l) after intravenous administration of pCAG/DT-A/FuGENE™6 complex. Note that increased deposition of mesangial matrix, as revealed by increased staining by PAM, was evident in the glomerulus on day 4 (f), but that in later stages (weeks 3 and 5) such deposits were decreased (i,l). (a-c) indicate intact kidney.

    Journal: BMC Nephrology

    Article Title: A new mouse model for renal lesions produced by intravenous injection of diphtheria toxin A-chain expression plasmid

    doi: 10.1186/1471-2369-5-4

    Figure Lengend Snippet: PAS and PAM staining of kidney sections sampled day 4 (d-f) and weeks 3 (g-i) and 5 (j-l) after intravenous administration of pCAG/DT-A/FuGENE™6 complex. Note that increased deposition of mesangial matrix, as revealed by increased staining by PAM, was evident in the glomerulus on day 4 (f), but that in later stages (weeks 3 and 5) such deposits were decreased (i,l). (a-c) indicate intact kidney.

    Article Snippet: For control injection (mock injection), a solution containing 40 μl of FuGENE™6 dissolved in PBS(-) (without DNA) or PBS(-) only was prepared.

    Techniques: Staining

    Effect of cytofectins on CT DNA uptake by macrophages. J774 cells were incubated for up to 48 hr with 0·4–10 µg/ml EMA-CT DNA with or without Fugene 6. DNA uptake was assessed by flow cytometric analysis of the cells collected at different time-points. The levels of DNA uptake are represented as mean fluorescence intensity (MFI) for each group. There was a dose- and time-dependent increase of DNA uptake in the presence of Fugene 6.

    Journal: Immunology

    Article Title: Effect of cytofectins on the immune response of murine macrophages to mammalian DNA

    doi: 10.1046/j.1365-2567.2003.01653.x

    Figure Lengend Snippet: Effect of cytofectins on CT DNA uptake by macrophages. J774 cells were incubated for up to 48 hr with 0·4–10 µg/ml EMA-CT DNA with or without Fugene 6. DNA uptake was assessed by flow cytometric analysis of the cells collected at different time-points. The levels of DNA uptake are represented as mean fluorescence intensity (MFI) for each group. There was a dose- and time-dependent increase of DNA uptake in the presence of Fugene 6.

    Article Snippet: Previous studies have shown that EC DNA is immunostimulatory, in contrast to CT DNA, which is inactive alone., In these experiments, J774 cells were treated with 10 µg/ml of ds and ss CT DNA or EC DNA alone or in complexes with Fugene 6 for up to 48 hr; supernatants were then examined for IL-12 and NO production.

    Techniques: Incubation, Flow Cytometry, Fluorescence

    Confocal microscopy analysis of intracellular distribution of CT DNA. J774 cells were simultaneously incubated with 1 µg/ml of EMA-labelled CT DNA in complexes with Fugene 6 (CT DNA) and 0·5 mg/ml Oregon green-labelled dextran (Dextran) at 37° for 12 hr. Internalized CT DNA (red-coloured) and dextran (green-coloured) were examined by confocal microscopy. CT DNA can be seen mostly co-localized (yellow-coloured) with dextran (Merged), indicating a fluid phase endocytosis pathway for DNA uptake (bar = 10 µm).

    Journal: Immunology

    Article Title: Effect of cytofectins on the immune response of murine macrophages to mammalian DNA

    doi: 10.1046/j.1365-2567.2003.01653.x

    Figure Lengend Snippet: Confocal microscopy analysis of intracellular distribution of CT DNA. J774 cells were simultaneously incubated with 1 µg/ml of EMA-labelled CT DNA in complexes with Fugene 6 (CT DNA) and 0·5 mg/ml Oregon green-labelled dextran (Dextran) at 37° for 12 hr. Internalized CT DNA (red-coloured) and dextran (green-coloured) were examined by confocal microscopy. CT DNA can be seen mostly co-localized (yellow-coloured) with dextran (Merged), indicating a fluid phase endocytosis pathway for DNA uptake (bar = 10 µm).

    Article Snippet: Previous studies have shown that EC DNA is immunostimulatory, in contrast to CT DNA, which is inactive alone., In these experiments, J774 cells were treated with 10 µg/ml of ds and ss CT DNA or EC DNA alone or in complexes with Fugene 6 for up to 48 hr; supernatants were then examined for IL-12 and NO production.

    Techniques: Confocal Microscopy, Incubation

    Effects of chloroquine on induction of NO by CT DNA–cytofectin complexes. J774 cells were pretreated with 1–4 µg/ml of chloroquine for 1 hr, followed by incubation with 10 µg/ml CT DNA in the presence of Fugene 6. EC DNA at 10 µg/ml and LPS at 1 µg/ml were used as controls. NO induction by CT DNA–cytofectin complexes was partially blocked by chloroquine. Whereas chloroquine blocked EC DNA-induced NO, it had a minimal effect on LPS activity. Error bars depict the standard deviations. The data are representative of at least three separate experiments. * P

    Journal: Immunology

    Article Title: Effect of cytofectins on the immune response of murine macrophages to mammalian DNA

    doi: 10.1046/j.1365-2567.2003.01653.x

    Figure Lengend Snippet: Effects of chloroquine on induction of NO by CT DNA–cytofectin complexes. J774 cells were pretreated with 1–4 µg/ml of chloroquine for 1 hr, followed by incubation with 10 µg/ml CT DNA in the presence of Fugene 6. EC DNA at 10 µg/ml and LPS at 1 µg/ml were used as controls. NO induction by CT DNA–cytofectin complexes was partially blocked by chloroquine. Whereas chloroquine blocked EC DNA-induced NO, it had a minimal effect on LPS activity. Error bars depict the standard deviations. The data are representative of at least three separate experiments. * P

    Article Snippet: Previous studies have shown that EC DNA is immunostimulatory, in contrast to CT DNA, which is inactive alone., In these experiments, J774 cells were treated with 10 µg/ml of ds and ss CT DNA or EC DNA alone or in complexes with Fugene 6 for up to 48 hr; supernatants were then examined for IL-12 and NO production.

    Techniques: Incubation, Activity Assay

    Z potential of control microparticles and of microparticles treated with PEI, LF2000 and FuGENE 6 ® . Note: The Zeta potential of the functionalized microparticle is also shown. Abbreviations: FMP, functionalized microparticle; PEI, polyethyleneimine; LF, Lipofectamine™.

    Journal: International Journal of Nanomedicine

    Article Title: Enhancing microparticle internalization by nonphagocytic cells through the use of noncovalently conjugated polyethyleneimine

    doi: 10.2147/IJN.S34635

    Figure Lengend Snippet: Z potential of control microparticles and of microparticles treated with PEI, LF2000 and FuGENE 6 ® . Note: The Zeta potential of the functionalized microparticle is also shown. Abbreviations: FMP, functionalized microparticle; PEI, polyethyleneimine; LF, Lipofectamine™.

    Article Snippet: PEI, LF2000, and FuGENE 6 cytotoxicity assay The cytotoxicity of PEI 25 KDa (Sigma-Aldrich, St Louis, MO) used at three different concentrations (0.05, 0.10, and 0.15 mM), of LF2000 (Life Technologies), and of FuGENE 6 (Promega Corporation, Fitchburg, WI), used at the concentrations recommended by the manufacturers, was evaluated after the transfection procedure by assessing two different parameters: the percentage of cells that remained attached to the dish (normalized to the control group), and the viability of the attached cells.

    Techniques:

    Transfection. Representative phase contrast and fluorescence images (10x) of ASSCs transfected with a GFP expression plasmid by different methods (Electroporation, LTX, PEI and Fugene HD), along with the efficiency of transfection as measured by cytometry. Each set of transfections was performed three times and standard deviations were negligible.

    Journal: PLoS ONE

    Article Title: Generation and Characterization of the First Immortalized Alpaca Cell Line Suitable for Diagnostic and Immunization Studies

    doi: 10.1371/journal.pone.0105643

    Figure Lengend Snippet: Transfection. Representative phase contrast and fluorescence images (10x) of ASSCs transfected with a GFP expression plasmid by different methods (Electroporation, LTX, PEI and Fugene HD), along with the efficiency of transfection as measured by cytometry. Each set of transfections was performed three times and standard deviations were negligible.

    Article Snippet: Therefore ASSCs were transfected by different methods [LTX (Invitrogen), PEI (Polyscience), Fugene HD (Promega) and electroporation] and the efficiency of transfection determined by image based-cytometry.

    Techniques: Transfection, Fluorescence, Expressing, Plasmid Preparation, Electroporation, Cytometry

    Silencing in GFP-expressing THP-1 cells. (A) The cell counts as measured by flow cytometry (expressed as a percentage of non-treated cells). ( B ) Silencing was assessed after 3 days of siRNA treatment (50 nM) and expressed as decrease in mean GFP fluorescence or decrease in GFP-positive cells. The polymer:siRNA ratios were 2∶1, 4∶1 and 8∶1. ( C ) Dose-response curves for GFP silencing between 25 and 200 nM siRNA treatment. The CA and LA substituted polymers were used at the polymer:siRNA ratios of 4∶1 and 8∶1, and silencing was assessed after 3 days of treatment. ( D ) Silencing by CA- and LA-substituted polymers and three commercial reagents (Lipofectamine™ 2000, Metafectamine and Fugene HD). The extent of silencing was summarized over a course of 9 days and expressed as decrease in mean GFP fluorescence (top panel) or decrease in GFP-positive cells (middle panel). The lipid substitutions of the polymers used were 2.1 LA/PEI (PEI2-LA) and 6.9 CA/PEI (PEI2-CA).

    Journal: PLoS ONE

    Article Title: Effective Non-Viral Delivery of siRNA to Acute Myeloid Leukemia Cells with Lipid-Substituted Polyethylenimines

    doi: 10.1371/journal.pone.0044197

    Figure Lengend Snippet: Silencing in GFP-expressing THP-1 cells. (A) The cell counts as measured by flow cytometry (expressed as a percentage of non-treated cells). ( B ) Silencing was assessed after 3 days of siRNA treatment (50 nM) and expressed as decrease in mean GFP fluorescence or decrease in GFP-positive cells. The polymer:siRNA ratios were 2∶1, 4∶1 and 8∶1. ( C ) Dose-response curves for GFP silencing between 25 and 200 nM siRNA treatment. The CA and LA substituted polymers were used at the polymer:siRNA ratios of 4∶1 and 8∶1, and silencing was assessed after 3 days of treatment. ( D ) Silencing by CA- and LA-substituted polymers and three commercial reagents (Lipofectamine™ 2000, Metafectamine and Fugene HD). The extent of silencing was summarized over a course of 9 days and expressed as decrease in mean GFP fluorescence (top panel) or decrease in GFP-positive cells (middle panel). The lipid substitutions of the polymers used were 2.1 LA/PEI (PEI2-LA) and 6.9 CA/PEI (PEI2-CA).

    Article Snippet: PEI25, PEI2-LA20 and RNAi-mate complexes were incubated for 30 min, Lipofectamine™ 2000, RNAiMAX™ and Metafectamine were incubated for 20 minutes, Fugene HD and DOTAP were incubated for 15 min and HiPerFect was incubated for 10 min, prior to drop-wise addition (100 µL) to cells in 200 µL of RPMI medium.

    Techniques: Expressing, Flow Cytometry, Cytometry, Fluorescence

    Activity of the pTα enhancer in various T cell lines. The 4.3- and 0.35-kb pTα enhancer fragments and the control CD3δ enhancer were subcloned upstream of the SV40 promoter/LacZ reporter gene. (A) The cells were transfected using Fugene 6 reagent. A CMV promoter/LacZ construct was used as a positive control for transfection of BW5147 cells. In B, the cells were transfected using Superfect reagent.

    Journal: The Journal of Experimental Medicine

    Article Title: Expression of the Mouse Pre-T Cell Receptor ? Gene Is Controlled by an Upstream Region Containing a Transcriptional Enhancer

    doi:

    Figure Lengend Snippet: Activity of the pTα enhancer in various T cell lines. The 4.3- and 0.35-kb pTα enhancer fragments and the control CD3δ enhancer were subcloned upstream of the SV40 promoter/LacZ reporter gene. (A) The cells were transfected using Fugene 6 reagent. A CMV promoter/LacZ construct was used as a positive control for transfection of BW5147 cells. In B, the cells were transfected using Superfect reagent.

    Article Snippet: Cell lines LR1, BW5147, MEL, and NIH3T3 were transfected using Fugene 6 reagent (Roche Molecular Biochemicals); cells from cell lines LR2 and 642 were transfected using Superfect reagent (Qiagen).

    Techniques: Activity Assay, Transfection, Construct, Positive Control