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    New England Biolabs fpg
    A) Fluorescence emission of 4 μM probe OGR1 in <t>Fpg</t> reaction buffer at 37 °C without Fpg (gray) and after 25 min with Fpg (black). B) Time course of 4 μM probe OGR1 emission at 465 nm following addition of 658 nM Fpg (black triangles) or <t>hOGG1</t> (gray circles) at 37 °C. This is a representative curve of n=1.
    Fpg, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 326 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fpg/product/New England Biolabs
    Average 99 stars, based on 326 article reviews
    Price from $9.99 to $1999.99
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    98
    New England Biolabs fpg reaction buffer
    A) Fluorescence emission of 4 μM probe OGR1 in <t>Fpg</t> reaction buffer at 37 °C without Fpg (gray) and after 25 min with Fpg (black). B) Time course of 4 μM probe OGR1 emission at 465 nm following addition of 658 nM Fpg (black triangles) or <t>hOGG1</t> (gray circles) at 37 °C. This is a representative curve of n=1.
    Fpg Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fpg reaction buffer/product/New England Biolabs
    Average 98 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    fpg reaction buffer - by Bioz Stars, 2020-07
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    97
    New England Biolabs modified purines formamidopyrimidine dna glycosylase fpg
    A) Fluorescence emission of 4 μM probe OGR1 in <t>Fpg</t> reaction buffer at 37 °C without Fpg (gray) and after 25 min with Fpg (black). B) Time course of 4 μM probe OGR1 emission at 465 nm following addition of 658 nM Fpg (black triangles) or <t>hOGG1</t> (gray circles) at 37 °C. This is a representative curve of n=1.
    Modified Purines Formamidopyrimidine Dna Glycosylase Fpg, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/modified purines formamidopyrimidine dna glycosylase fpg/product/New England Biolabs
    Average 97 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    modified purines formamidopyrimidine dna glycosylase fpg - by Bioz Stars, 2020-07
    97/100 stars
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    Image Search Results


    A) Fluorescence emission of 4 μM probe OGR1 in Fpg reaction buffer at 37 °C without Fpg (gray) and after 25 min with Fpg (black). B) Time course of 4 μM probe OGR1 emission at 465 nm following addition of 658 nM Fpg (black triangles) or hOGG1 (gray circles) at 37 °C. This is a representative curve of n=1.

    Journal: Chembiochem : a European journal of chemical biology

    Article Title: In Vitro Fluorogenic Real-time Assay of the Repair of Oxidative DNA Damage

    doi: 10.1002/cbic.201500184

    Figure Lengend Snippet: A) Fluorescence emission of 4 μM probe OGR1 in Fpg reaction buffer at 37 °C without Fpg (gray) and after 25 min with Fpg (black). B) Time course of 4 μM probe OGR1 emission at 465 nm following addition of 658 nM Fpg (black triangles) or hOGG1 (gray circles) at 37 °C. This is a representative curve of n=1.

    Article Snippet: Fpg, hOGG1, Fpg reaction buffer (NEB buffer 1: 10 mM Bis-Tris-Propane-HCl, 10 mM MgCl2 , 1 mM dithiothreitol, pH 7.0 at 25 °C), hOGG1 reaction buffer (NEB buffer 4: 50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate, 1 mM dithiothreitol, pH 7.9 at 25 °C), and BSA (10 mg/mL) were purchased from New England Biolabs.

    Techniques: Fluorescence

    A and B) PAGE analysis of 4 μM probe OGR1 radiolabeled at the 5′ end with [ 32 P]-phosphate and reacted with 658 nM hOGG1 (A) or Fpg (B) at 37 °C for various times. Piperidine (200 mM, 95 °C, 5 min), which cleaves the DNA backbone at abasic sites, was added to samples as indicated to identify the 8-oxoguanine excision product and the backbone cleavage product. C and D) MALDI mass spectrometry analysis of probe OGR1 following overnight reaction with hOGG1 (C) or Fpg (D).

    Journal: Chembiochem : a European journal of chemical biology

    Article Title: In Vitro Fluorogenic Real-time Assay of the Repair of Oxidative DNA Damage

    doi: 10.1002/cbic.201500184

    Figure Lengend Snippet: A and B) PAGE analysis of 4 μM probe OGR1 radiolabeled at the 5′ end with [ 32 P]-phosphate and reacted with 658 nM hOGG1 (A) or Fpg (B) at 37 °C for various times. Piperidine (200 mM, 95 °C, 5 min), which cleaves the DNA backbone at abasic sites, was added to samples as indicated to identify the 8-oxoguanine excision product and the backbone cleavage product. C and D) MALDI mass spectrometry analysis of probe OGR1 following overnight reaction with hOGG1 (C) or Fpg (D).

    Article Snippet: Fpg, hOGG1, Fpg reaction buffer (NEB buffer 1: 10 mM Bis-Tris-Propane-HCl, 10 mM MgCl2 , 1 mM dithiothreitol, pH 7.0 at 25 °C), hOGG1 reaction buffer (NEB buffer 4: 50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate, 1 mM dithiothreitol, pH 7.9 at 25 °C), and BSA (10 mg/mL) were purchased from New England Biolabs.

    Techniques: Polyacrylamide Gel Electrophoresis, Mass Spectrometry

    ARP discriminates between AP sites generated by enzymatic and nonenzymatic release of N7-meG. DNA substrates (20 nM) were a 9:1 mixture of homoduplex G:C and heteroduplex AP:C generated either by spontaneous N7-meG depurination (nonenzymatic, blue triangles), or N7-meG excision by hAAG (enzymatic, red squares). Substrates were incubated either with purified proteins [( A ) FPG: 0.5 nM; ( C ) ARP: 10 nM] or Arabidopsis cell-free extracts [( B ) arp −/− : 8 µg; ( D ) fpg −/− : 8 µg]. Reactions for detection of AP endonuclease activity ( C and D ) were supplemented with 2 mM MgCl 2 . After stabilization with NaBH 4 , reaction products were separated by denaturing PAGE and detected by fluorescence scanning. Values are means with SEs from three independent experiments.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Nonenzymatic release of N7-methylguanine channels repair of abasic sites into an AP endonuclease-independent pathway in Arabidopsis

    doi: 10.1073/pnas.1719497115

    Figure Lengend Snippet: ARP discriminates between AP sites generated by enzymatic and nonenzymatic release of N7-meG. DNA substrates (20 nM) were a 9:1 mixture of homoduplex G:C and heteroduplex AP:C generated either by spontaneous N7-meG depurination (nonenzymatic, blue triangles), or N7-meG excision by hAAG (enzymatic, red squares). Substrates were incubated either with purified proteins [( A ) FPG: 0.5 nM; ( C ) ARP: 10 nM] or Arabidopsis cell-free extracts [( B ) arp −/− : 8 µg; ( D ) fpg −/− : 8 µg]. Reactions for detection of AP endonuclease activity ( C and D ) were supplemented with 2 mM MgCl 2 . After stabilization with NaBH 4 , reaction products were separated by denaturing PAGE and detected by fluorescence scanning. Values are means with SEs from three independent experiments.

    Article Snippet: Validation of DNA Substrates Containing N7-meG and Me-FAPy-G. DNA substrates (20 nM) containing N7-meG or me-FAPy-G were validated in reactions (50 µL) with hAAG (2 U; NEB), hAPE1 (1 U; NEB), or E. coli Fpg (EcoFpg, 8 U; NEB).

    Techniques: Generated, Incubation, Purification, Activity Assay, Polyacrylamide Gel Electrophoresis, Fluorescence

    FPG incises AP sites generated by spontaneous depurination of N7-meG. ( A ) Double-stranded oligonucleotide substrates (20 nM) containing a single lesion opposite C (N7-meG, me-FAPy-G, or AP site generated by uracil excision) were incubated for 2 h at 37 °C with Arabidopsis FPG (10 nM), hAPE1 (1 U), hAAG (2 U), or E. coli Fpg (8 U). ( B ) A double-stranded oligonucleotide substrate containing a single N7-meG:C pair was preincubated in DNA incision assay buffer ( Methods ) at 37 °C for the indicated times and then treated with human APE1 (1 U), E. coli Fpg (8 U), or Arabidopsis FPG (10 nM) for 1 h at 37 °C, or with NaOH (15 mM) for 10 min. at 70 °C. After stabilization with NaBH 4, reaction products were separated by denaturing PAGE and detected by fluorescence scanning. Values are means with SEs from three independent experiments.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Nonenzymatic release of N7-methylguanine channels repair of abasic sites into an AP endonuclease-independent pathway in Arabidopsis

    doi: 10.1073/pnas.1719497115

    Figure Lengend Snippet: FPG incises AP sites generated by spontaneous depurination of N7-meG. ( A ) Double-stranded oligonucleotide substrates (20 nM) containing a single lesion opposite C (N7-meG, me-FAPy-G, or AP site generated by uracil excision) were incubated for 2 h at 37 °C with Arabidopsis FPG (10 nM), hAPE1 (1 U), hAAG (2 U), or E. coli Fpg (8 U). ( B ) A double-stranded oligonucleotide substrate containing a single N7-meG:C pair was preincubated in DNA incision assay buffer ( Methods ) at 37 °C for the indicated times and then treated with human APE1 (1 U), E. coli Fpg (8 U), or Arabidopsis FPG (10 nM) for 1 h at 37 °C, or with NaOH (15 mM) for 10 min. at 70 °C. After stabilization with NaBH 4, reaction products were separated by denaturing PAGE and detected by fluorescence scanning. Values are means with SEs from three independent experiments.

    Article Snippet: Validation of DNA Substrates Containing N7-meG and Me-FAPy-G. DNA substrates (20 nM) containing N7-meG or me-FAPy-G were validated in reactions (50 µL) with hAAG (2 U; NEB), hAPE1 (1 U; NEB), or E. coli Fpg (EcoFpg, 8 U; NEB).

    Techniques: Generated, Incubation, Polyacrylamide Gel Electrophoresis, Fluorescence

    Depurinated N7-meG is repaired through FPG-dependent SP-BER. DNA substrates (20 nM) were a 9:1 mixture of homoduplex G:C and heteroduplex AP:C generated either by uracil excision (enzymatic AP:C, Left ) or by spontaneous N7-meG depurination (nonenzymatic AP:C, Right ). Similar concentrations of each type of AP site were verified by incision with hAPE1 (10 U) (lanes 2 and 17). Substrates were incubated with Arabidopsis cell-free extracts (8 µg) for 1 h at 37 °C with or without either dGTP or all four dNTPs. After stabilization with NaBH 4 , reaction products were separated by denaturing PAGE and detected by fluorescence scanning. N.E., nonextract.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Nonenzymatic release of N7-methylguanine channels repair of abasic sites into an AP endonuclease-independent pathway in Arabidopsis

    doi: 10.1073/pnas.1719497115

    Figure Lengend Snippet: Depurinated N7-meG is repaired through FPG-dependent SP-BER. DNA substrates (20 nM) were a 9:1 mixture of homoduplex G:C and heteroduplex AP:C generated either by uracil excision (enzymatic AP:C, Left ) or by spontaneous N7-meG depurination (nonenzymatic AP:C, Right ). Similar concentrations of each type of AP site were verified by incision with hAPE1 (10 U) (lanes 2 and 17). Substrates were incubated with Arabidopsis cell-free extracts (8 µg) for 1 h at 37 °C with or without either dGTP or all four dNTPs. After stabilization with NaBH 4 , reaction products were separated by denaturing PAGE and detected by fluorescence scanning. N.E., nonextract.

    Article Snippet: Validation of DNA Substrates Containing N7-meG and Me-FAPy-G. DNA substrates (20 nM) containing N7-meG or me-FAPy-G were validated in reactions (50 µL) with hAAG (2 U; NEB), hAPE1 (1 U; NEB), or E. coli Fpg (EcoFpg, 8 U; NEB).

    Techniques: Generated, Incubation, Polyacrylamide Gel Electrophoresis, Fluorescence

    A) Fluorescence emission of 4 μM probe OGR1 in Fpg reaction buffer at 37 °C without Fpg (gray) and after 25 min with Fpg (black). B) Time course of 4 μM probe OGR1 emission at 465 nm following addition of 658 nM Fpg (black triangles) or hOGG1 (gray circles) at 37 °C. This is a representative curve of n=1.

    Journal: Chembiochem : a European journal of chemical biology

    Article Title: In Vitro Fluorogenic Real-time Assay of the Repair of Oxidative DNA Damage

    doi: 10.1002/cbic.201500184

    Figure Lengend Snippet: A) Fluorescence emission of 4 μM probe OGR1 in Fpg reaction buffer at 37 °C without Fpg (gray) and after 25 min with Fpg (black). B) Time course of 4 μM probe OGR1 emission at 465 nm following addition of 658 nM Fpg (black triangles) or hOGG1 (gray circles) at 37 °C. This is a representative curve of n=1.

    Article Snippet: Fpg, hOGG1, Fpg reaction buffer (NEB buffer 1: 10 mM Bis-Tris-Propane-HCl, 10 mM MgCl2 , 1 mM dithiothreitol, pH 7.0 at 25 °C), hOGG1 reaction buffer (NEB buffer 4: 50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate, 1 mM dithiothreitol, pH 7.9 at 25 °C), and BSA (10 mg/mL) were purchased from New England Biolabs.

    Techniques: Fluorescence

    A and B) PAGE analysis of 4 μM probe OGR1 radiolabeled at the 5′ end with [ 32 P]-phosphate and reacted with 658 nM hOGG1 (A) or Fpg (B) at 37 °C for various times. Piperidine (200 mM, 95 °C, 5 min), which cleaves the DNA backbone at abasic sites, was added to samples as indicated to identify the 8-oxoguanine excision product and the backbone cleavage product. C and D) MALDI mass spectrometry analysis of probe OGR1 following overnight reaction with hOGG1 (C) or Fpg (D).

    Journal: Chembiochem : a European journal of chemical biology

    Article Title: In Vitro Fluorogenic Real-time Assay of the Repair of Oxidative DNA Damage

    doi: 10.1002/cbic.201500184

    Figure Lengend Snippet: A and B) PAGE analysis of 4 μM probe OGR1 radiolabeled at the 5′ end with [ 32 P]-phosphate and reacted with 658 nM hOGG1 (A) or Fpg (B) at 37 °C for various times. Piperidine (200 mM, 95 °C, 5 min), which cleaves the DNA backbone at abasic sites, was added to samples as indicated to identify the 8-oxoguanine excision product and the backbone cleavage product. C and D) MALDI mass spectrometry analysis of probe OGR1 following overnight reaction with hOGG1 (C) or Fpg (D).

    Article Snippet: Fpg, hOGG1, Fpg reaction buffer (NEB buffer 1: 10 mM Bis-Tris-Propane-HCl, 10 mM MgCl2 , 1 mM dithiothreitol, pH 7.0 at 25 °C), hOGG1 reaction buffer (NEB buffer 4: 50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate, 1 mM dithiothreitol, pH 7.9 at 25 °C), and BSA (10 mg/mL) were purchased from New England Biolabs.

    Techniques: Polyacrylamide Gel Electrophoresis, Mass Spectrometry