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  • 86
    Chembridge compound sri 29365
    EC 50 and CC 50 plots for <t>SRI</t> <t>29365</t> in HEp-2 and A549 cells. EC 50 and CC 50 values were calculated for SRI 29365 against wt hRSV in either (A) HEp-2 cells or (B) A549 cells. In panel C, the concentration-dependent antiviral and cytotoxic responses were calculated for SRI 29365 against wt hRSV in HEp-2 cells. An eight point concentration dose response cytoprotection assay was performed (compound concentration range of 100–0.04 μg/mL) in the presence of hRSV-infected cells and the percent of cell viability (compared to uninfected, undrugged cell controls) was plotted v. test compound concentration (solid line). Each concentration point was performed in triplicate. In parallel, the same concentration range of test compound was added to uninfected cells to determine compound-induced cytotoxicity (dotted line). EC 50 /CC 50 values were converted to Log M (molar) concentrations ( x -axis). Dose–response curves were plotted, and EC 50 values were calculated using IDBS’ XLfit function 205 [ y = A + (( B − A )/(1 + (( C / x )^ D )))] with minimum ( A ) and maximum ( B ) parameters set at 0 and 100 respectively.
    Compound Sri 29365, supplied by Chembridge, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/compound sri 29365/product/Chembridge
    Average 86 stars, based on 1 article reviews
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    86
    Thermo Fisher adk2
    EC 50 and CC 50 plots for <t>SRI</t> <t>29365</t> in HEp-2 and A549 cells. EC 50 and CC 50 values were calculated for SRI 29365 against wt hRSV in either (A) HEp-2 cells or (B) A549 cells. In panel C, the concentration-dependent antiviral and cytotoxic responses were calculated for SRI 29365 against wt hRSV in HEp-2 cells. An eight point concentration dose response cytoprotection assay was performed (compound concentration range of 100–0.04 μg/mL) in the presence of hRSV-infected cells and the percent of cell viability (compared to uninfected, undrugged cell controls) was plotted v. test compound concentration (solid line). Each concentration point was performed in triplicate. In parallel, the same concentration range of test compound was added to uninfected cells to determine compound-induced cytotoxicity (dotted line). EC 50 /CC 50 values were converted to Log M (molar) concentrations ( x -axis). Dose–response curves were plotted, and EC 50 values were calculated using IDBS’ XLfit function 205 [ y = A + (( B − A )/(1 + (( C / x )^ D )))] with minimum ( A ) and maximum ( B ) parameters set at 0 and 100 respectively.
    Adk2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adk2/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
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    93
    Santa Cruz Biotechnology ikkα sirna
    EC 50 and CC 50 plots for <t>SRI</t> <t>29365</t> in HEp-2 and A549 cells. EC 50 and CC 50 values were calculated for SRI 29365 against wt hRSV in either (A) HEp-2 cells or (B) A549 cells. In panel C, the concentration-dependent antiviral and cytotoxic responses were calculated for SRI 29365 against wt hRSV in HEp-2 cells. An eight point concentration dose response cytoprotection assay was performed (compound concentration range of 100–0.04 μg/mL) in the presence of hRSV-infected cells and the percent of cell viability (compared to uninfected, undrugged cell controls) was plotted v. test compound concentration (solid line). Each concentration point was performed in triplicate. In parallel, the same concentration range of test compound was added to uninfected cells to determine compound-induced cytotoxicity (dotted line). EC 50 /CC 50 values were converted to Log M (molar) concentrations ( x -axis). Dose–response curves were plotted, and EC 50 values were calculated using IDBS’ XLfit function 205 [ y = A + (( B − A )/(1 + (( C / x )^ D )))] with minimum ( A ) and maximum ( B ) parameters set at 0 and 100 respectively.
    Ikkα Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Santa Cruz Biotechnology ikkα
    EC 50 and CC 50 plots for <t>SRI</t> <t>29365</t> in HEp-2 and A549 cells. EC 50 and CC 50 values were calculated for SRI 29365 against wt hRSV in either (A) HEp-2 cells or (B) A549 cells. In panel C, the concentration-dependent antiviral and cytotoxic responses were calculated for SRI 29365 against wt hRSV in HEp-2 cells. An eight point concentration dose response cytoprotection assay was performed (compound concentration range of 100–0.04 μg/mL) in the presence of hRSV-infected cells and the percent of cell viability (compared to uninfected, undrugged cell controls) was plotted v. test compound concentration (solid line). Each concentration point was performed in triplicate. In parallel, the same concentration range of test compound was added to uninfected cells to determine compound-induced cytotoxicity (dotted line). EC 50 /CC 50 values were converted to Log M (molar) concentrations ( x -axis). Dose–response curves were plotted, and EC 50 values were calculated using IDBS’ XLfit function 205 [ y = A + (( B − A )/(1 + (( C / x )^ D )))] with minimum ( A ) and maximum ( B ) parameters set at 0 and 100 respectively.
    Ikkα, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ikkα/product/Santa Cruz Biotechnology
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    85
    Santa Cruz Biotechnology ikk α shrna plasmid mouse
    EC 50 and CC 50 plots for <t>SRI</t> <t>29365</t> in HEp-2 and A549 cells. EC 50 and CC 50 values were calculated for SRI 29365 against wt hRSV in either (A) HEp-2 cells or (B) A549 cells. In panel C, the concentration-dependent antiviral and cytotoxic responses were calculated for SRI 29365 against wt hRSV in HEp-2 cells. An eight point concentration dose response cytoprotection assay was performed (compound concentration range of 100–0.04 μg/mL) in the presence of hRSV-infected cells and the percent of cell viability (compared to uninfected, undrugged cell controls) was plotted v. test compound concentration (solid line). Each concentration point was performed in triplicate. In parallel, the same concentration range of test compound was added to uninfected cells to determine compound-induced cytotoxicity (dotted line). EC 50 /CC 50 values were converted to Log M (molar) concentrations ( x -axis). Dose–response curves were plotted, and EC 50 values were calculated using IDBS’ XLfit function 205 [ y = A + (( B − A )/(1 + (( C / x )^ D )))] with minimum ( A ) and maximum ( B ) parameters set at 0 and 100 respectively.
    Ikk α Shrna Plasmid Mouse, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ikk α shrna plasmid mouse/product/Santa Cruz Biotechnology
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    Image Search Results


    EC 50 and CC 50 plots for SRI 29365 in HEp-2 and A549 cells. EC 50 and CC 50 values were calculated for SRI 29365 against wt hRSV in either (A) HEp-2 cells or (B) A549 cells. In panel C, the concentration-dependent antiviral and cytotoxic responses were calculated for SRI 29365 against wt hRSV in HEp-2 cells. An eight point concentration dose response cytoprotection assay was performed (compound concentration range of 100–0.04 μg/mL) in the presence of hRSV-infected cells and the percent of cell viability (compared to uninfected, undrugged cell controls) was plotted v. test compound concentration (solid line). Each concentration point was performed in triplicate. In parallel, the same concentration range of test compound was added to uninfected cells to determine compound-induced cytotoxicity (dotted line). EC 50 /CC 50 values were converted to Log M (molar) concentrations ( x -axis). Dose–response curves were plotted, and EC 50 values were calculated using IDBS’ XLfit function 205 [ y = A + (( B − A )/(1 + (( C / x )^ D )))] with minimum ( A ) and maximum ( B ) parameters set at 0 and 100 respectively.

    Journal: Antiviral Research

    Article Title: Benzimidazole analogs inhibit respiratory syncytial virus G protein function

    doi: 10.1016/j.antiviral.2015.06.016

    Figure Lengend Snippet: EC 50 and CC 50 plots for SRI 29365 in HEp-2 and A549 cells. EC 50 and CC 50 values were calculated for SRI 29365 against wt hRSV in either (A) HEp-2 cells or (B) A549 cells. In panel C, the concentration-dependent antiviral and cytotoxic responses were calculated for SRI 29365 against wt hRSV in HEp-2 cells. An eight point concentration dose response cytoprotection assay was performed (compound concentration range of 100–0.04 μg/mL) in the presence of hRSV-infected cells and the percent of cell viability (compared to uninfected, undrugged cell controls) was plotted v. test compound concentration (solid line). Each concentration point was performed in triplicate. In parallel, the same concentration range of test compound was added to uninfected cells to determine compound-induced cytotoxicity (dotted line). EC 50 /CC 50 values were converted to Log M (molar) concentrations ( x -axis). Dose–response curves were plotted, and EC 50 values were calculated using IDBS’ XLfit function 205 [ y = A + (( B − A )/(1 + (( C / x )^ D )))] with minimum ( A ) and maximum ( B ) parameters set at 0 and 100 respectively.

    Article Snippet: Compound SRI 29365 (140 mg, Chembridge, San Diego, CA) was supplied as dry powder and was diluted in 7 mL of PBS (20 mg/mL).

    Techniques: Concentration Assay, Infection

    Time-of-addition assays for SRI 29365. SRI 29365 (5 μM) was added to 96-well microplates containing a confluent monolayer of HEp-2 cells at timepoints of −1, 0, 1, 2, 4, 8 h, post viral infection of the cells with 1 MOI of hRSV Long strain. After a four-day incubation, cell viability was determined (compared to uninfected, undrugged cell controls) ( n = 3). The graph shows the change in cell viability as the compound addition timepoint changes, and demonstrates that SRI 29365 did not reduce CPE more than 50% if added at 2 h post-infection.

    Journal: Antiviral Research

    Article Title: Benzimidazole analogs inhibit respiratory syncytial virus G protein function

    doi: 10.1016/j.antiviral.2015.06.016

    Figure Lengend Snippet: Time-of-addition assays for SRI 29365. SRI 29365 (5 μM) was added to 96-well microplates containing a confluent monolayer of HEp-2 cells at timepoints of −1, 0, 1, 2, 4, 8 h, post viral infection of the cells with 1 MOI of hRSV Long strain. After a four-day incubation, cell viability was determined (compared to uninfected, undrugged cell controls) ( n = 3). The graph shows the change in cell viability as the compound addition timepoint changes, and demonstrates that SRI 29365 did not reduce CPE more than 50% if added at 2 h post-infection.

    Article Snippet: Compound SRI 29365 (140 mg, Chembridge, San Diego, CA) was supplied as dry powder and was diluted in 7 mL of PBS (20 mg/mL).

    Techniques: Infection, Incubation

    An A63V mutation in the hRSV G protein grants resistance to SRI 29365. Panel A shows the hRSV G protein genetic sequence trace of the wt hRSV and Panel B shows the genetic trace of the SRI 29365-resistant mutant hRSV, which shows a C → T mutation at position 500 in the sequence trace. Panel C shows the hRSV G protein domain structure and the location of the translated A63V amino acid mutation at the interface of the transmembrane domain and the virion surface domain. Panel D shows the amino acid sequence conservation of the region of the G protein transmembrane domain flanking residue A63 in RSVA Long (wild-type), RSVA Long (SRI-29365-resistant mutant), RSVA Tracy, and RSVB WI/629-12/06-07.

    Journal: Antiviral Research

    Article Title: Benzimidazole analogs inhibit respiratory syncytial virus G protein function

    doi: 10.1016/j.antiviral.2015.06.016

    Figure Lengend Snippet: An A63V mutation in the hRSV G protein grants resistance to SRI 29365. Panel A shows the hRSV G protein genetic sequence trace of the wt hRSV and Panel B shows the genetic trace of the SRI 29365-resistant mutant hRSV, which shows a C → T mutation at position 500 in the sequence trace. Panel C shows the hRSV G protein domain structure and the location of the translated A63V amino acid mutation at the interface of the transmembrane domain and the virion surface domain. Panel D shows the amino acid sequence conservation of the region of the G protein transmembrane domain flanking residue A63 in RSVA Long (wild-type), RSVA Long (SRI-29365-resistant mutant), RSVA Tracy, and RSVB WI/629-12/06-07.

    Article Snippet: Compound SRI 29365 (140 mg, Chembridge, San Diego, CA) was supplied as dry powder and was diluted in 7 mL of PBS (20 mg/mL).

    Techniques: Mutagenesis, Sequencing

    SRI 29365 has differential efficacy in HEp-2, A549, and Vero E6 cells. Confluent monolayers of HEp-2, A549, or Vero E6 cells with either the RSVA Long strain wild-type or SRI-29356-resistant mutant in the presence of 5 μM SRI 29365, and the reduction in virus titer (TCID 50 reduction) was determined relative to infected (-compound) control cells ( n = 4). The compound reduced wt hRSV virus TCID50 values by 300-fold, but only 10-fold in A549 cells, and less than 10-fold in Vero E6 cells. SRI 29365 reduced mutant hRSV virus TCID 50 values by less than 10-fold in all cell types.

    Journal: Antiviral Research

    Article Title: Benzimidazole analogs inhibit respiratory syncytial virus G protein function

    doi: 10.1016/j.antiviral.2015.06.016

    Figure Lengend Snippet: SRI 29365 has differential efficacy in HEp-2, A549, and Vero E6 cells. Confluent monolayers of HEp-2, A549, or Vero E6 cells with either the RSVA Long strain wild-type or SRI-29356-resistant mutant in the presence of 5 μM SRI 29365, and the reduction in virus titer (TCID 50 reduction) was determined relative to infected (-compound) control cells ( n = 4). The compound reduced wt hRSV virus TCID50 values by 300-fold, but only 10-fold in A549 cells, and less than 10-fold in Vero E6 cells. SRI 29365 reduced mutant hRSV virus TCID 50 values by less than 10-fold in all cell types.

    Article Snippet: Compound SRI 29365 (140 mg, Chembridge, San Diego, CA) was supplied as dry powder and was diluted in 7 mL of PBS (20 mg/mL).

    Techniques: Mutagenesis, Infection