fluorophore-labeled hydrolysis probes Search Results


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    Vector Laboratories dylight 594 labeled lycopersicon esculentum tomato lectin
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    Thermo Fisher superscript indirect cdna labeling system
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    Thermo Fisher alexa fluor 546
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    Vector Laboratories dylight 649 labeled lycopersicon esculentum tomato lectin
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    Vector Laboratories dylight 488 labeled lycopersicon esculentum lectin
    Dylight 488 Labeled Lycopersicon Esculentum Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher alexa fluor 488 labeled tfp ester
    Characterization of <t>Alexa</t> Fluor ® 488-labeled Aβ42 oligomers by SDS-PAGE and AFM. (A) Fluorescently labeled Aβ42 oligomers appear similar to unlabeled Aβ42 by gel/blot analysis. Oligomers formed from unlabeled Aβ42
    Alexa Fluor 488 Labeled Tfp Ester, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher bodipy fl labeled casein
    Characterization of <t>Alexa</t> Fluor ® 488-labeled Aβ42 oligomers by SDS-PAGE and AFM. (A) Fluorescently labeled Aβ42 oligomers appear similar to unlabeled Aβ42 by gel/blot analysis. Oligomers formed from unlabeled Aβ42
    Bodipy Fl Labeled Casein, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 6 carboxyfluorescein fam
    Characterization of <t>Alexa</t> Fluor ® 488-labeled Aβ42 oligomers by SDS-PAGE and AFM. (A) Fluorescently labeled Aβ42 oligomers appear similar to unlabeled Aβ42 by gel/blot analysis. Oligomers formed from unlabeled Aβ42
    6 Carboxyfluorescein Fam, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 561 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore fluorescein isothiocyanate
    Characterization of <t>Alexa</t> Fluor ® 488-labeled Aβ42 oligomers by SDS-PAGE and AFM. (A) Fluorescently labeled Aβ42 oligomers appear similar to unlabeled Aβ42 by gel/blot analysis. Oligomers formed from unlabeled Aβ42
    Fluorescein Isothiocyanate, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 4279 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher platinum dna polymerase
    Characterization of <t>Alexa</t> Fluor ® 488-labeled Aβ42 oligomers by SDS-PAGE and AFM. (A) Fluorescently labeled Aβ42 oligomers appear similar to unlabeled Aβ42 by gel/blot analysis. Oligomers formed from unlabeled Aβ42
    Platinum Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 209 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quantstudio dx
    Analytical performance comparison between MP96 and LC 2.0 RNA extraction platforms in serum and urine. a Normal human serum or b urine pooled from healthy donors was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of each dilution were extracted using the small volume protocol (0.2 mL) and were tested with the Trioplex assay on the ABI7500 Fast Dx instrument. The same serum ( c ) and urine ( d ) dilutions were tested on the <t>QuantStudio</t> Dx instrument. Mean genome copy equivalents per milliliter (GCE/mL) of viral RNA detected are displayed at each dilution. Error bars represent GCE/mL standard deviation. Straight line represents linear regression of MP96 platform (Roche). Dashed line represents linear regression of LC 2.0 platform (Roche)
    Quantstudio Dx, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher abi 7500 fast dx
    Clinical performance of the Trioplex assay across specimen types. Clinical specimens collected concurrently from 373 cases with previous Zika determination in the acute stage were tested. RNA was extracted with the MagNA Pure 96 small volume external lysis protocol from 373 case-paired serum, 373 urine, and 345 whole blood-EDTA specimens and tested with the Trioplex assay in multiplex format in the <t>ABI</t> 7500 Fast Dx instrument. a Correlation of CT values between case-matching serum and urine specimens; R 2 = 0.36 p
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    Kaneka Corp polymerase activity
    Clinical performance of the Trioplex assay across specimen types. Clinical specimens collected concurrently from 373 cases with previous Zika determination in the acute stage were tested. RNA was extracted with the MagNA Pure 96 small volume external lysis protocol from 373 case-paired serum, 373 urine, and 345 whole blood-EDTA specimens and tested with the Trioplex assay in multiplex format in the <t>ABI</t> 7500 Fast Dx instrument. a Correlation of CT values between case-matching serum and urine specimens; R 2 = 0.36 p
    Polymerase Activity, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad probes
    Clinical performance of the Trioplex assay across specimen types. Clinical specimens collected concurrently from 373 cases with previous Zika determination in the acute stage were tested. RNA was extracted with the MagNA Pure 96 small volume external lysis protocol from 373 case-paired serum, 373 urine, and 345 whole blood-EDTA specimens and tested with the Trioplex assay in multiplex format in the <t>ABI</t> 7500 Fast Dx instrument. a Correlation of CT values between case-matching serum and urine specimens; R 2 = 0.36 p
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    Bio-Rad autodgtm system
    Clinical performance of the Trioplex assay across specimen types. Clinical specimens collected concurrently from 373 cases with previous Zika determination in the acute stage were tested. RNA was extracted with the MagNA Pure 96 small volume external lysis protocol from 373 case-paired serum, 373 urine, and 345 whole blood-EDTA specimens and tested with the Trioplex assay in multiplex format in the <t>ABI</t> 7500 Fast Dx instrument. a Correlation of CT values between case-matching serum and urine specimens; R 2 = 0.36 p
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    Integrated DNA Technologies hexachlorofluorescein hex reporter fluorophores
    Clinical performance of the Trioplex assay across specimen types. Clinical specimens collected concurrently from 373 cases with previous Zika determination in the acute stage were tested. RNA was extracted with the MagNA Pure 96 small volume external lysis protocol from 373 case-paired serum, 373 urine, and 345 whole blood-EDTA specimens and tested with the Trioplex assay in multiplex format in the <t>ABI</t> 7500 Fast Dx instrument. a Correlation of CT values between case-matching serum and urine specimens; R 2 = 0.36 p
    Hexachlorofluorescein Hex Reporter Fluorophores, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dq bsa
    Clinical performance of the Trioplex assay across specimen types. Clinical specimens collected concurrently from 373 cases with previous Zika determination in the acute stage were tested. RNA was extracted with the MagNA Pure 96 small volume external lysis protocol from 373 case-paired serum, 373 urine, and 345 whole blood-EDTA specimens and tested with the Trioplex assay in multiplex format in the <t>ABI</t> 7500 Fast Dx instrument. a Correlation of CT values between case-matching serum and urine specimens; R 2 = 0.36 p
    Dq Bsa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 305 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad bio rad ddpcr supermix
    Clinical performance of the Trioplex assay across specimen types. Clinical specimens collected concurrently from 373 cases with previous Zika determination in the acute stage were tested. RNA was extracted with the MagNA Pure 96 small volume external lysis protocol from 373 case-paired serum, 373 urine, and 345 whole blood-EDTA specimens and tested with the Trioplex assay in multiplex format in the <t>ABI</t> 7500 Fast Dx instrument. a Correlation of CT values between case-matching serum and urine specimens; R 2 = 0.36 p
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    Image Search Results


    Characterization of Alexa Fluor ® 488-labeled Aβ42 oligomers by SDS-PAGE and AFM. (A) Fluorescently labeled Aβ42 oligomers appear similar to unlabeled Aβ42 by gel/blot analysis. Oligomers formed from unlabeled Aβ42

    Journal: Journal of molecular recognition : JMR

    Article Title: Preparation of fluorescently-labeled amyloid-beta peptide assemblies: the effect of fluorophore conjugation on structure and function

    doi: 10.1002/jmr.948

    Figure Lengend Snippet: Characterization of Alexa Fluor ® 488-labeled Aβ42 oligomers by SDS-PAGE and AFM. (A) Fluorescently labeled Aβ42 oligomers appear similar to unlabeled Aβ42 by gel/blot analysis. Oligomers formed from unlabeled Aβ42

    Article Snippet: After 24 h incubation at 4°C, labeling was performed using the Alexa Fluor® 488 TFP ester Microscale Labeling Kit ( , Invitrogen) essentially according to manufacturer protocol.

    Techniques: Labeling, SDS Page, Western Blot

    Following uptake, Alexa Fluor ® 488-labeled Aβ42 oligomers appear as punctate fluorescence within the cell, similar to unlabeled Aβ42 oligomers. Aβ uptake in Neuro-2A cells was analyzed using confocal laser scanning microscopy

    Journal: Journal of molecular recognition : JMR

    Article Title: Preparation of fluorescently-labeled amyloid-beta peptide assemblies: the effect of fluorophore conjugation on structure and function

    doi: 10.1002/jmr.948

    Figure Lengend Snippet: Following uptake, Alexa Fluor ® 488-labeled Aβ42 oligomers appear as punctate fluorescence within the cell, similar to unlabeled Aβ42 oligomers. Aβ uptake in Neuro-2A cells was analyzed using confocal laser scanning microscopy

    Article Snippet: After 24 h incubation at 4°C, labeling was performed using the Alexa Fluor® 488 TFP ester Microscale Labeling Kit ( , Invitrogen) essentially according to manufacturer protocol.

    Techniques: Labeling, Fluorescence, Confocal Laser Scanning Microscopy

    Analytical performance comparison between MP96 and LC 2.0 RNA extraction platforms in serum and urine. a Normal human serum or b urine pooled from healthy donors was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of each dilution were extracted using the small volume protocol (0.2 mL) and were tested with the Trioplex assay on the ABI7500 Fast Dx instrument. The same serum ( c ) and urine ( d ) dilutions were tested on the QuantStudio Dx instrument. Mean genome copy equivalents per milliliter (GCE/mL) of viral RNA detected are displayed at each dilution. Error bars represent GCE/mL standard deviation. Straight line represents linear regression of MP96 platform (Roche). Dashed line represents linear regression of LC 2.0 platform (Roche)

    Journal: Nature Communications

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

    doi: 10.1038/s41467-018-03772-1

    Figure Lengend Snippet: Analytical performance comparison between MP96 and LC 2.0 RNA extraction platforms in serum and urine. a Normal human serum or b urine pooled from healthy donors was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of each dilution were extracted using the small volume protocol (0.2 mL) and were tested with the Trioplex assay on the ABI7500 Fast Dx instrument. The same serum ( c ) and urine ( d ) dilutions were tested on the QuantStudio Dx instrument. Mean genome copy equivalents per milliliter (GCE/mL) of viral RNA detected are displayed at each dilution. Error bars represent GCE/mL standard deviation. Straight line represents linear regression of MP96 platform (Roche). Dashed line represents linear regression of LC 2.0 platform (Roche)

    Article Snippet: The fluorophore-labeled hydrolysis probes bind to the amplified DNA target fragment and the intensity of the fluorescent signal is captured by a real-time PCR instrument: (ABI 7500 fast Dx (ThermoFisher) or QuantStudio Dx (ThermoFisher)).

    Techniques: RNA Extraction, Standard Deviation

    Analytical performance comparison between Trioplex assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the ABI 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions

    Journal: Nature Communications

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

    doi: 10.1038/s41467-018-03772-1

    Figure Lengend Snippet: Analytical performance comparison between Trioplex assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the ABI 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions

    Article Snippet: The fluorophore-labeled hydrolysis probes bind to the amplified DNA target fragment and the intensity of the fluorescent signal is captured by a real-time PCR instrument: (ABI 7500 fast Dx (ThermoFisher) or QuantStudio Dx (ThermoFisher)).

    Techniques: Multiplex Assay, RNA Extraction, Polymerase Chain Reaction, Singleplex Assay, Standard Deviation

    Analytical performance comparison between MP96 and LC 2.0 RNA extraction platforms in whole blood (EDTA). A pool of whole blood donated by healthy donors was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD) and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicas of every dilution were extracted using small volume protocol (0.2 mL) and tested with Trioplex assay on the ABI7500 Fast Dx instrument ( a ) or in the QuantStudio Dx instrument ( b ). Mean genome copy equivalents per milliliter (GCE/mL) of viral RNA detected are displayed at each dilution. Error bars represent GCE/mL standard deviation. Straight line represents linear regression of MP96 platform (Roche). Dashed line represents linear regression of LC 2.0 platform (Roche)

    Journal: Nature Communications

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

    doi: 10.1038/s41467-018-03772-1

    Figure Lengend Snippet: Analytical performance comparison between MP96 and LC 2.0 RNA extraction platforms in whole blood (EDTA). A pool of whole blood donated by healthy donors was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD) and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicas of every dilution were extracted using small volume protocol (0.2 mL) and tested with Trioplex assay on the ABI7500 Fast Dx instrument ( a ) or in the QuantStudio Dx instrument ( b ). Mean genome copy equivalents per milliliter (GCE/mL) of viral RNA detected are displayed at each dilution. Error bars represent GCE/mL standard deviation. Straight line represents linear regression of MP96 platform (Roche). Dashed line represents linear regression of LC 2.0 platform (Roche)

    Article Snippet: The fluorophore-labeled hydrolysis probes bind to the amplified DNA target fragment and the intensity of the fluorescent signal is captured by a real-time PCR instrument: (ABI 7500 fast Dx (ThermoFisher) or QuantStudio Dx (ThermoFisher)).

    Techniques: RNA Extraction, Standard Deviation

    Clinical performance of the Trioplex assay across specimen types. Clinical specimens collected concurrently from 373 cases with previous Zika determination in the acute stage were tested. RNA was extracted with the MagNA Pure 96 small volume external lysis protocol from 373 case-paired serum, 373 urine, and 345 whole blood-EDTA specimens and tested with the Trioplex assay in multiplex format in the ABI 7500 Fast Dx instrument. a Correlation of CT values between case-matching serum and urine specimens; R 2 = 0.36 p

    Journal: Nature Communications

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

    doi: 10.1038/s41467-018-03772-1

    Figure Lengend Snippet: Clinical performance of the Trioplex assay across specimen types. Clinical specimens collected concurrently from 373 cases with previous Zika determination in the acute stage were tested. RNA was extracted with the MagNA Pure 96 small volume external lysis protocol from 373 case-paired serum, 373 urine, and 345 whole blood-EDTA specimens and tested with the Trioplex assay in multiplex format in the ABI 7500 Fast Dx instrument. a Correlation of CT values between case-matching serum and urine specimens; R 2 = 0.36 p

    Article Snippet: The fluorophore-labeled hydrolysis probes bind to the amplified DNA target fragment and the intensity of the fluorescent signal is captured by a real-time PCR instrument: (ABI 7500 fast Dx (ThermoFisher) or QuantStudio Dx (ThermoFisher)).

    Techniques: Lysis, Multiplex Assay

    Analytical performance comparison between Trioplex assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the ABI 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions

    Journal: Nature Communications

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

    doi: 10.1038/s41467-018-03772-1

    Figure Lengend Snippet: Analytical performance comparison between Trioplex assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the ABI 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions

    Article Snippet: The fluorophore-labeled hydrolysis probes bind to the amplified DNA target fragment and the intensity of the fluorescent signal is captured by a real-time PCR instrument: (ABI 7500 fast Dx (ThermoFisher) or QuantStudio Dx (ThermoFisher)).

    Techniques: Multiplex Assay, RNA Extraction, Polymerase Chain Reaction, Singleplex Assay, Standard Deviation