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    BPS Bioscience fluorogenic hdac1 assay kit
    LHC and isolated <t>HDAC1</t> deacetylation of nucleosomes and isolated histone H3 containing Kac. ( A ) His-FLAG-HDAC1 (50 nM) showed no detectable deacetylation of H3K9ac nucleosome substrate (100 nM). ( B ) His-FLAG-HDAC1 (5 nM) showed robust deacetylation of both H3K9ac and H3K14ac histone H3 substrates (1 μM). ( C ) Deacetylation of the H3K9K14K18 triacetylated histone H3 (1 μM) by 1 nM LHC. ( D ) Deacetylation of 100 nM H3K9ac nucleosomes (lacking H3K4me2 modification) by 20 nM LHC. Each of these panels shows western blots with the relevant site-specific and total H3 Abs. (n = 3 for all measurements except n = 2 for A and B).
    Fluorogenic Hdac1 Assay Kit, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorogenic hdac1 assay kit/product/BPS Bioscience
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorogenic hdac1 assay kit - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    86
    BPS Bioscience hdac1 fluorogenic assay kit
    LHC and isolated <t>HDAC1</t> deacetylation of nucleosomes and isolated histone H3 containing Kac. ( A ) His-FLAG-HDAC1 (50 nM) showed no detectable deacetylation of H3K9ac nucleosome substrate (100 nM). ( B ) His-FLAG-HDAC1 (5 nM) showed robust deacetylation of both H3K9ac and H3K14ac histone H3 substrates (1 μM). ( C ) Deacetylation of the H3K9K14K18 triacetylated histone H3 (1 μM) by 1 nM LHC. ( D ) Deacetylation of 100 nM H3K9ac nucleosomes (lacking H3K4me2 modification) by 20 nM LHC. Each of these panels shows western blots with the relevant site-specific and total H3 Abs. (n = 3 for all measurements except n = 2 for A and B).
    Hdac1 Fluorogenic Assay Kit, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hdac1 fluorogenic assay kit/product/BPS Bioscience
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hdac1 fluorogenic assay kit - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    Image Search Results


    LHC and isolated HDAC1 deacetylation of nucleosomes and isolated histone H3 containing Kac. ( A ) His-FLAG-HDAC1 (50 nM) showed no detectable deacetylation of H3K9ac nucleosome substrate (100 nM). ( B ) His-FLAG-HDAC1 (5 nM) showed robust deacetylation of both H3K9ac and H3K14ac histone H3 substrates (1 μM). ( C ) Deacetylation of the H3K9K14K18 triacetylated histone H3 (1 μM) by 1 nM LHC. ( D ) Deacetylation of 100 nM H3K9ac nucleosomes (lacking H3K4me2 modification) by 20 nM LHC. Each of these panels shows western blots with the relevant site-specific and total H3 Abs. (n = 3 for all measurements except n = 2 for A and B).

    Journal: eLife

    Article Title: Lysine-14 acetylation of histone H3 in chromatin confers resistance to the deacetylase and demethylase activities of an epigenetic silencing complex

    doi: 10.7554/eLife.37231

    Figure Lengend Snippet: LHC and isolated HDAC1 deacetylation of nucleosomes and isolated histone H3 containing Kac. ( A ) His-FLAG-HDAC1 (50 nM) showed no detectable deacetylation of H3K9ac nucleosome substrate (100 nM). ( B ) His-FLAG-HDAC1 (5 nM) showed robust deacetylation of both H3K9ac and H3K14ac histone H3 substrates (1 μM). ( C ) Deacetylation of the H3K9K14K18 triacetylated histone H3 (1 μM) by 1 nM LHC. ( D ) Deacetylation of 100 nM H3K9ac nucleosomes (lacking H3K4me2 modification) by 20 nM LHC. Each of these panels shows western blots with the relevant site-specific and total H3 Abs. (n = 3 for all measurements except n = 2 for A and B).

    Article Snippet: His-FLAG-HDAC1 and Fluorogenic HDAC1 assay kit was purchased from BPS Bioscience (San Diego, CA).

    Techniques: Isolation, Modification, Western Blot

    Demethylation kinetics of H3K4me2 nucleosomes (100 nM) by LHC (400 nM) quantified by western blots. ( A ) The H3K4me2 nucleosome with 185 bp dsDNA was slowly demethylated by the CoREST complex while nucleosome demethylation with with 146 bp dsDNA was below the level of detection. ( B ) The LSD1 and HDAC1 dual inhibitor Corin (20 μM) inhibited the demethylation of the 185 bp nucleosome substrate; (n = 3 for A and n = 2 for B); error bars represent S.E.M.

    Journal: eLife

    Article Title: Lysine-14 acetylation of histone H3 in chromatin confers resistance to the deacetylase and demethylase activities of an epigenetic silencing complex

    doi: 10.7554/eLife.37231

    Figure Lengend Snippet: Demethylation kinetics of H3K4me2 nucleosomes (100 nM) by LHC (400 nM) quantified by western blots. ( A ) The H3K4me2 nucleosome with 185 bp dsDNA was slowly demethylated by the CoREST complex while nucleosome demethylation with with 146 bp dsDNA was below the level of detection. ( B ) The LSD1 and HDAC1 dual inhibitor Corin (20 μM) inhibited the demethylation of the 185 bp nucleosome substrate; (n = 3 for A and n = 2 for B); error bars represent S.E.M.

    Article Snippet: His-FLAG-HDAC1 and Fluorogenic HDAC1 assay kit was purchased from BPS Bioscience (San Diego, CA).

    Techniques: Western Blot

    The CoREST complex and its H3 substrate histone modifications. ( A ) The CoREST complex (LHC) represses gene transcription by erasing histone modifications on nucleosomes. LSD1 demethylates H3K4me2 and HDAC1 deacetylates multiple Kac sites on histones which are activating marks. ( B ) The sequence of the N-terminal histone H3 tail and the Kme2 and Kac modifications studied in this paper. The short recognition sequence of F40 sortase is labelled in green.

    Journal: eLife

    Article Title: Lysine-14 acetylation of histone H3 in chromatin confers resistance to the deacetylase and demethylase activities of an epigenetic silencing complex

    doi: 10.7554/eLife.37231

    Figure Lengend Snippet: The CoREST complex and its H3 substrate histone modifications. ( A ) The CoREST complex (LHC) represses gene transcription by erasing histone modifications on nucleosomes. LSD1 demethylates H3K4me2 and HDAC1 deacetylates multiple Kac sites on histones which are activating marks. ( B ) The sequence of the N-terminal histone H3 tail and the Kme2 and Kac modifications studied in this paper. The short recognition sequence of F40 sortase is labelled in green.

    Article Snippet: His-FLAG-HDAC1 and Fluorogenic HDAC1 assay kit was purchased from BPS Bioscience (San Diego, CA).

    Techniques: Sequencing

    Quantification and curve fitting of the deacetylation assays. ( A ) Deacetylation of 1 μM Kac histone H3 by 1 nM LHC. ( B ) Deacetylation of 100 nM H3K9K14K18 triacetylated nucleosomes by 40 nM LHC. ( C ) Deacetylation of 1 μM H3K9K14K18 triacetylated histone H3 by 1 nM LHC. ( D ) Deacetylation of 100 nM H3K9ac nucleosomes without H3K4me2 by 20 nM LHC. V/[E]=0.14 ± 0.0045 min −1 . ( E ) The deacetylation of 1 μM Kac histone H3 by 5 nM His-FLAG-HDAC1; (n = 3 for all measurements except n = 2 for A and C); Error bars represent S.E.M.

    Journal: eLife

    Article Title: Lysine-14 acetylation of histone H3 in chromatin confers resistance to the deacetylase and demethylase activities of an epigenetic silencing complex

    doi: 10.7554/eLife.37231

    Figure Lengend Snippet: Quantification and curve fitting of the deacetylation assays. ( A ) Deacetylation of 1 μM Kac histone H3 by 1 nM LHC. ( B ) Deacetylation of 100 nM H3K9K14K18 triacetylated nucleosomes by 40 nM LHC. ( C ) Deacetylation of 1 μM H3K9K14K18 triacetylated histone H3 by 1 nM LHC. ( D ) Deacetylation of 100 nM H3K9ac nucleosomes without H3K4me2 by 20 nM LHC. V/[E]=0.14 ± 0.0045 min −1 . ( E ) The deacetylation of 1 μM Kac histone H3 by 5 nM His-FLAG-HDAC1; (n = 3 for all measurements except n = 2 for A and C); Error bars represent S.E.M.

    Article Snippet: His-FLAG-HDAC1 and Fluorogenic HDAC1 assay kit was purchased from BPS Bioscience (San Diego, CA).

    Techniques: