fluorescein labeled l pha Search Results


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    Vector Laboratories fitc labeled l pha
    CTD110.6 recognizes both O- GlcNAc and terminal β-GlcNAc on cell surface glycoproteins. A , N- ), of swainsonine. B , binding of <t>L-PHA</t> ( upper ) and mAb CTD110.6 ( lower ) to fixed cells. Lec2 and Lec8 cells were cultured with or without 5 μg/ml swainsonine ( SW ) for 4 days. Cells (4 × 10 5 ) were incubated with 20 μg/ml fluorescein-labeled L-PHA or 40 μg/ml CTD110.6 on ice for 20 min followed by 10 μg/ml Cy5-conjugated anti-mouse IgM antibody. Gray profiles , secondary Abs; thin line , control cells; bold line , swainsonine-treated cells. Representative results are from two independent experiments.
    Fitc Labeled L Pha, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    fitc labeled l pha - by Bioz Stars, 2022-10
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    CTD110.6 recognizes both O- GlcNAc and terminal β-GlcNAc on cell surface glycoproteins. A , N- ), of swainsonine. B , binding of L-PHA ( upper ) and mAb CTD110.6 ( lower ) to fixed cells. Lec2 and Lec8 cells were cultured with or without 5 μg/ml swainsonine ( SW ) for 4 days. Cells (4 × 10 5 ) were incubated with 20 μg/ml fluorescein-labeled L-PHA or 40 μg/ml CTD110.6 on ice for 20 min followed by 10 μg/ml Cy5-conjugated anti-mouse IgM antibody. Gray profiles , secondary Abs; thin line , control cells; bold line , swainsonine-treated cells. Representative results are from two independent experiments.

    Journal: The Journal of Biological Chemistry

    Article Title: Antibodies That Detect O-Linked β-d-N-Acetylglucosamine on the Extracellular Domain of Cell Surface Glycoproteins *

    doi: 10.1074/jbc.M113.492512

    Figure Lengend Snippet: CTD110.6 recognizes both O- GlcNAc and terminal β-GlcNAc on cell surface glycoproteins. A , N- ), of swainsonine. B , binding of L-PHA ( upper ) and mAb CTD110.6 ( lower ) to fixed cells. Lec2 and Lec8 cells were cultured with or without 5 μg/ml swainsonine ( SW ) for 4 days. Cells (4 × 10 5 ) were incubated with 20 μg/ml fluorescein-labeled L-PHA or 40 μg/ml CTD110.6 on ice for 20 min followed by 10 μg/ml Cy5-conjugated anti-mouse IgM antibody. Gray profiles , secondary Abs; thin line , control cells; bold line , swainsonine-treated cells. Representative results are from two independent experiments.

    Article Snippet: Washed cells were incubated with 50 μl of binding buffer containing 1 μg of fluorescein-labeled L-PHA ( Phaseolus vulgaris leukoagglutinin; Vector, Burlingame, CA) or 2 μg of CTD110.6 mAb, followed by incubation with 50 μl of binding buffer containing 0.5 μg of Cy5-conjugated anti-mouse IgM.

    Techniques: Binding Assay, Cell Culture, Incubation, Labeling

    Characterization of Lec1 cells phenotype. CHO-K1 ( B ) and Lec1 ( A ) cells were fixed in 2% paraformaldehyde, blocked in TBS containing 10% goat serum, and then incubated with FITC-labeled lectin L-PHA, with nuclei counterstained with DAPI. Cells were visualized under Leica confocal fluorescence microscope (TCS SP2). CHO-K1 (red line) and Lec1 cells (black line) were incubated with FITC-labeled L-PHA for 45 min at 4°C and analyzed by flow cytometry. Cells analyzed in the absence of FITC-labeled L-PHA are shown in the filled light-gray trace ( C ). CHO-K1 ( E ) and Lec1 ( D ) cells were fixed in 2% paraformaldehyde, blocked in TBS containing 10% goat serum, and then incubated with DIG-labeled lectin GNA at 37°C. After 45 min incubation, cells were incubated with AP-conjugated anti-DIG for 45 min at 37°C and then treated with NBT/BCIP for visualization under Leical fluorescence microscope. CHO-K1 (red line) and Lec1 cells (black line) were incubated with the DIG-labeled lectin GNA for 45 min at 4°C and incubated with FITC-conjugated anti-DIG for 45 min at 37°C and then analyzed by flow cytometry. Cells analyzed in the absence of lectin are shown in the filled light-gray trace ( F ).

    Journal: Glycobiology

    Article Title: Hybrid- and complex-type N-glycans are not essential for Newcastle disease virus infection and fusion of host cells

    doi: 10.1093/glycob/cwr146

    Figure Lengend Snippet: Characterization of Lec1 cells phenotype. CHO-K1 ( B ) and Lec1 ( A ) cells were fixed in 2% paraformaldehyde, blocked in TBS containing 10% goat serum, and then incubated with FITC-labeled lectin L-PHA, with nuclei counterstained with DAPI. Cells were visualized under Leica confocal fluorescence microscope (TCS SP2). CHO-K1 (red line) and Lec1 cells (black line) were incubated with FITC-labeled L-PHA for 45 min at 4°C and analyzed by flow cytometry. Cells analyzed in the absence of FITC-labeled L-PHA are shown in the filled light-gray trace ( C ). CHO-K1 ( E ) and Lec1 ( D ) cells were fixed in 2% paraformaldehyde, blocked in TBS containing 10% goat serum, and then incubated with DIG-labeled lectin GNA at 37°C. After 45 min incubation, cells were incubated with AP-conjugated anti-DIG for 45 min at 37°C and then treated with NBT/BCIP for visualization under Leical fluorescence microscope. CHO-K1 (red line) and Lec1 cells (black line) were incubated with the DIG-labeled lectin GNA for 45 min at 4°C and incubated with FITC-conjugated anti-DIG for 45 min at 37°C and then analyzed by flow cytometry. Cells analyzed in the absence of lectin are shown in the filled light-gray trace ( F ).

    Article Snippet: Lectin-binding assays used fluorescein isothiocyanate (FITC)-labeled L-PHA (Vector Laboratories, Burlingame, CA), and digoxigenin (DIG)-labeled MAA, SNA and GNA (Roche Diagnostics, Indianapolis, IN).

    Techniques: Incubation, Labeling, Fluorescence, Microscopy, Flow Cytometry, Cytometry