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  • 99
    Thermo Fisher fluo3 ref
    Fluo3 Ref, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluo3 ref/product/Thermo Fisher
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    fluo3 ref - by Bioz Stars, 2020-08
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    95
    Millipore fluo3 acetoxymethyl ester fluo3 am
    Effect of glibenclamide on intracellular calcium levels in E . granulosus protoscoleces. (A) Protoscoleces were incubated with buffer (Co), 200 μM glibenclamide (Glb) or 10μM trifluoperazine (TFP, positive control) for 2 h and then loaded with <t>Fluo3-AM</t> ester solution in darkness. Data are the mean ± S.D. of three independent experiments. *Statistically significant difference ( p
    Fluo3 Acetoxymethyl Ester Fluo3 Am, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluo3 acetoxymethyl ester fluo3 am/product/Millipore
    Average 95 stars, based on 62 article reviews
    Price from $9.99 to $1999.99
    fluo3 acetoxymethyl ester fluo3 am - by Bioz Stars, 2020-08
    95/100 stars
      Buy from Supplier

    94
    Millipore fluo 3 am
    Effects of wortmannin and YD-3 on intracellular calcium mobilization in platelets. <t>Fluo-3-loaded</t> human platelets were incubated with DMSO, wortmannin (Wort, 200 nM) and/or YD-3 (20 µM) at 37°C for 5 min in the presence of 1 mM extracellular Ca 2+ , then thrombin (0.1 U·mL −1 ), PAR1-AP (20 µM) or PAR4-AP (100 µM) was added to trigger the increase of [Ca 2+ ] i . Results are representatives of four independent experiments and are quantified in the histograms. Values are mean ± SEM ( n = 4). * P
    Fluo 3 Am, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 535 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluo 3 am/product/Millipore
    Average 94 stars, based on 535 article reviews
    Price from $9.99 to $1999.99
    fluo 3 am - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    Image Search Results


    Effect of glibenclamide on intracellular calcium levels in E . granulosus protoscoleces. (A) Protoscoleces were incubated with buffer (Co), 200 μM glibenclamide (Glb) or 10μM trifluoperazine (TFP, positive control) for 2 h and then loaded with Fluo3-AM ester solution in darkness. Data are the mean ± S.D. of three independent experiments. *Statistically significant difference ( p

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Anthelminthic activity of glibenclamide on secondary cystic echinococcosis in mice

    doi: 10.1371/journal.pntd.0006111

    Figure Lengend Snippet: Effect of glibenclamide on intracellular calcium levels in E . granulosus protoscoleces. (A) Protoscoleces were incubated with buffer (Co), 200 μM glibenclamide (Glb) or 10μM trifluoperazine (TFP, positive control) for 2 h and then loaded with Fluo3-AM ester solution in darkness. Data are the mean ± S.D. of three independent experiments. *Statistically significant difference ( p

    Article Snippet: Experiments were carried out with 5 x 103 protoscoleces incubated under control conditions or treated with 200 μM Glb for 2 h. Then, the parasites were incubated with a solution containing 10 μM Fluo3-AM, 1 mM CaCl2 , 0.1% v/v pluronic F-127 and 2 mM probenecid (Sigma, USA) for 30 min at 4° C in the dark.

    Techniques: Incubation, Positive Control

    Intracellular Ca 2+ release by MCF-7 human breast cancer cells treated with SIgA adsorbed to PEG microspheres. Notes: Cells were loaded with the fluorescent radiometric calcium indicator Fluo3-acetoxymethyl (Fluo3-AM; Sigma-Aldrich Co., St Louis, MO, USA) and Ca 2+ release was determined by immunofluorescence assay and flow cytometry (FACSCalibur; BD Biosciences, San Jose, CA, USA). Flow cytometric histogram shows the intensities of green fluorescence (FL1-H) on the x-axis and the cell counts on the y-axis. Abbreviations: SIgA, secretory immunoglobulin A; PEG, polyethylene glycol.

    Journal: OncoTargets and therapy

    Article Title: Intracellular calcium is a target of modulation of apoptosis in MCF-7 cells in the presence of IgA adsorbed to polyethylene glycol

    doi: 10.2147/OTT.S99839

    Figure Lengend Snippet: Intracellular Ca 2+ release by MCF-7 human breast cancer cells treated with SIgA adsorbed to PEG microspheres. Notes: Cells were loaded with the fluorescent radiometric calcium indicator Fluo3-acetoxymethyl (Fluo3-AM; Sigma-Aldrich Co., St Louis, MO, USA) and Ca 2+ release was determined by immunofluorescence assay and flow cytometry (FACSCalibur; BD Biosciences, San Jose, CA, USA). Flow cytometric histogram shows the intensities of green fluorescence (FL1-H) on the x-axis and the cell counts on the y-axis. Abbreviations: SIgA, secretory immunoglobulin A; PEG, polyethylene glycol.

    Article Snippet: Cells were loaded with the fluorescent radiometric calcium indicator fluo3-acetoxymethyl (Fluo3-AM; Sigma-Aldrich Co.).

    Techniques: Immunofluorescence, Flow Cytometry, Cytometry, Fluorescence

    Ca 2+ mobilization triggered by MCP-1, CCR2–02 mAb, or crosslinked CCR2–02 Fab. Induction of Ca 2+ mobilization was promoted by 10 nM MCP-1 (Peprotech) alone or in the presence of PTX ( A and D , respectively), 15 nM CCR2–02 mAb ( B and E ), and 1 μg/ml of CCR2–02 Fab plus 50 μg/ml of rabbit anti-mouse IgG Fab ( C and F ) in Mono Mac 1 cells loaded with Fluo-3,AM, as described. Results are expressed as a percentage of the chemokine-induced calcium response. Shown are the results of one representative experiment of five performed. ( G - I ) Ca 2+ mobilization promoted by 1 μg/ml of CCR2–02 Fab alone, 50 μg/ml of rabbit anti-mouse IgG Fab alone, and 1 μg/ml of isotype-matched control Fab plus 50 μg/ml of rabbit anti-mouse IgG Fab. Fab and anti-Fab antibody were added sequentially; cells were washed between treatments. Arrows indicate stimulus addition.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: The chemokine monocyte chemoattractant protein-1 induces functional responses through dimerization of its receptor CCR2

    doi:

    Figure Lengend Snippet: Ca 2+ mobilization triggered by MCP-1, CCR2–02 mAb, or crosslinked CCR2–02 Fab. Induction of Ca 2+ mobilization was promoted by 10 nM MCP-1 (Peprotech) alone or in the presence of PTX ( A and D , respectively), 15 nM CCR2–02 mAb ( B and E ), and 1 μg/ml of CCR2–02 Fab plus 50 μg/ml of rabbit anti-mouse IgG Fab ( C and F ) in Mono Mac 1 cells loaded with Fluo-3,AM, as described. Results are expressed as a percentage of the chemokine-induced calcium response. Shown are the results of one representative experiment of five performed. ( G - I ) Ca 2+ mobilization promoted by 1 μg/ml of CCR2–02 Fab alone, 50 μg/ml of rabbit anti-mouse IgG Fab alone, and 1 μg/ml of isotype-matched control Fab plus 50 μg/ml of rabbit anti-mouse IgG Fab. Fab and anti-Fab antibody were added sequentially; cells were washed between treatments. Arrows indicate stimulus addition.

    Article Snippet: Intracellular calcium concentration changes were monitored by using the fluorescent probe Fluo-3,AM (Calbiochem), as described ( ).

    Techniques:

    The effect of Microcystin-LR (MC-LR) on intracellular Ca 2+ concentration . Intracellular Ca 2+ levels were measured in different groups by Fluo-3/AM fluorescent probes through flow cytometry. (A) 0 μM, (B) 2.5 μM, (C) 5 μM, (D) 10 μM, (E) Bars represent the mean values of three replications ± SD. * P

    Journal: Frontiers in Physiology

    Article Title: Novel Role of ER Stress and Autophagy in Microcystin-LR Induced Apoptosis in Chinese Hamster Ovary Cells

    doi: 10.3389/fphys.2016.00527

    Figure Lengend Snippet: The effect of Microcystin-LR (MC-LR) on intracellular Ca 2+ concentration . Intracellular Ca 2+ levels were measured in different groups by Fluo-3/AM fluorescent probes through flow cytometry. (A) 0 μM, (B) 2.5 μM, (C) 5 μM, (D) 10 μM, (E) Bars represent the mean values of three replications ± SD. * P

    Article Snippet: Briefly, CHO cells were exposed to different concentrations of MC-LR (0, 2.5, 5, 10 μM), and then loaded with the Ca2+ fluorescent probe fluo-3 AM (Sigma, St. Louis, MO, USA) for 30 min at 37°C.

    Techniques: Concentration Assay, Flow Cytometry, Cytometry

    Calcium wave activity is suppressed by cytochalasin D in established astrocytic cultures. Confluent astrocytic cultures were loaded with fluo-3, and calcium waves were evoked by mechanical stimulation. Upper row , A control untreated culture propagates a calcium wave that includes > 25 cells. Middle row , Astrocytes pretreated with cytochalasin D for 5 min only propagate calcium increases to a few astrocytes. Lower row , Culture treated with cytochalasin D for 10 min was not capable of propagating a calcium wave, despite a robust calcium increment in the stimulated cell. Actin fibers were visualized in the same field ( right , in red ) by fixing and staining with Texas Red–phalloidin immediately after analysis of calcium signaling. Note the gradual disappearance of stress fibers in cytochalasin D-treated cultures. Fluorescence intensities of the fluo-3 probe corresponding to relative calcium levels were monitored 1, 7, 15, and 23 sec after stimulation by confocal microscopy. In all frames, background counts were subtracted. Scale bar, 10 μm.

    Journal: The Journal of Neuroscience

    Article Title: Cytoskeletal Assembly and ATP Release Regulate Astrocytic Calcium Signaling

    doi: 10.1523/JNEUROSCI.18-21-08794.1998

    Figure Lengend Snippet: Calcium wave activity is suppressed by cytochalasin D in established astrocytic cultures. Confluent astrocytic cultures were loaded with fluo-3, and calcium waves were evoked by mechanical stimulation. Upper row , A control untreated culture propagates a calcium wave that includes > 25 cells. Middle row , Astrocytes pretreated with cytochalasin D for 5 min only propagate calcium increases to a few astrocytes. Lower row , Culture treated with cytochalasin D for 10 min was not capable of propagating a calcium wave, despite a robust calcium increment in the stimulated cell. Actin fibers were visualized in the same field ( right , in red ) by fixing and staining with Texas Red–phalloidin immediately after analysis of calcium signaling. Note the gradual disappearance of stress fibers in cytochalasin D-treated cultures. Fluorescence intensities of the fluo-3 probe corresponding to relative calcium levels were monitored 1, 7, 15, and 23 sec after stimulation by confocal microscopy. In all frames, background counts were subtracted. Scale bar, 10 μm.

    Article Snippet: After fluo-3 resting signal was recorded, 25 μ m ATP or 25 μ m bradykinin (Sigma) was applied.

    Techniques: Activity Assay, Staining, Fluorescence, Size-exclusion Chromatography, Confocal Microscopy

    Astrocytes from mice with a Cx43 null mutation propagate robust Ca 2+ waves. Upper row , Mechanical stimulation induced a calcium wave that propagated > 300 μm from the stimulated cell in astrocytes from a Cx43 knock-out mouse. The culture was loaded with fluo-3. Lower row , Texas Red–phalloidin staining of a sister culture revealed well-organized actin fiber bundles. Actin organization in Cx43 knock-out mice did not differ from wild-type astrocytes. Scale bar: Upper row , 75 μm; lower row , 7 μm.

    Journal: The Journal of Neuroscience

    Article Title: Cytoskeletal Assembly and ATP Release Regulate Astrocytic Calcium Signaling

    doi: 10.1523/JNEUROSCI.18-21-08794.1998

    Figure Lengend Snippet: Astrocytes from mice with a Cx43 null mutation propagate robust Ca 2+ waves. Upper row , Mechanical stimulation induced a calcium wave that propagated > 300 μm from the stimulated cell in astrocytes from a Cx43 knock-out mouse. The culture was loaded with fluo-3. Lower row , Texas Red–phalloidin staining of a sister culture revealed well-organized actin fiber bundles. Actin organization in Cx43 knock-out mice did not differ from wild-type astrocytes. Scale bar: Upper row , 75 μm; lower row , 7 μm.

    Article Snippet: After fluo-3 resting signal was recorded, 25 μ m ATP or 25 μ m bradykinin (Sigma) was applied.

    Techniques: Mouse Assay, Mutagenesis, Knock-Out, Staining

    Astrocytic calcium signaling increases as a function of time in culture. Upper row , One hour after plating, mechanical stimulation triggers a robust calcium increase only in the stimulated cell ( red arrows ) but fails to initiate a calcium wave. Middle row , Eight hours after plating, calcium waves propagated to include several neighboring cells. Lower row , Twenty-four hours after plating, the wave included astrocytes located as far as 300 μm from the stimulation site. Confluent astrocytic cultures were loaded with fluo-3, and calcium waves were evoked by mechanical stimulation. Fluorescence intensities of fluo-3 were monitored 1, 7, 15, and 23 sec after stimulation by confocal microscopy. In all frames, background counts were subtracted. Scale bar, 50 μm.

    Journal: The Journal of Neuroscience

    Article Title: Cytoskeletal Assembly and ATP Release Regulate Astrocytic Calcium Signaling

    doi: 10.1523/JNEUROSCI.18-21-08794.1998

    Figure Lengend Snippet: Astrocytic calcium signaling increases as a function of time in culture. Upper row , One hour after plating, mechanical stimulation triggers a robust calcium increase only in the stimulated cell ( red arrows ) but fails to initiate a calcium wave. Middle row , Eight hours after plating, calcium waves propagated to include several neighboring cells. Lower row , Twenty-four hours after plating, the wave included astrocytes located as far as 300 μm from the stimulation site. Confluent astrocytic cultures were loaded with fluo-3, and calcium waves were evoked by mechanical stimulation. Fluorescence intensities of fluo-3 were monitored 1, 7, 15, and 23 sec after stimulation by confocal microscopy. In all frames, background counts were subtracted. Scale bar, 50 μm.

    Article Snippet: After fluo-3 resting signal was recorded, 25 μ m ATP or 25 μ m bradykinin (Sigma) was applied.

    Techniques: Fluorescence, Size-exclusion Chromatography, Confocal Microscopy

    Effects of Ca 2+ channel inhibitor LaCl 3 on the cytosolic Ca 2+ concentration and cellulase production. A Fluorescence analysis of LaCl 3 influence on cytosolic Ca 2+ burst induced by Mn 2+ . The T. reesei Rut-C30 were cultured in liquid minimal medium for 48–60 h with 0 or 10 mM MnCl 2 (0 or 10 Mn, respectively), and then treated with 0 or 5 mM LaCl 3 . For detection, 50 μM Fluo-3/AM was used, and the intensity was monitored using Automatic Inverted Fluorescence Microscopy. Green fluorescence represents the free cytosolic Ca 2+ . DIC, differential interference contrast, CK, not treated with LaCl 3 . B Comparative fluorescence ratio analysis of LaCl 3 influence on the cytosolic Ca 2+ burst induced by Mn 2+ . The y -axis represents the Ca 2+ fluorescence ratio measured by CLSM and the x- axis the different treatments. The CMCase activity ( C ) and p NPCase activity ( D ) of T. reesei Rut-C30 were examined after culture in medium containing 0 or 10 mM MnCl 2 and with (−) or without (+) 5 mM LaCl 3 . The expression levels of cbh1 ( E ) and egl1 ( F ) in T. reesei Rut-C30 were analyzed after culture in medium containing 0 or 10 mM MnCl 2 and with (−) or without (+) 5 mM LaCl 3 . Values are the mean ± SD of the results from three independent experiments. Different letters indicate significant differences between the columns ( p

    Journal: Biotechnology for Biofuels

    Article Title: Mn2+ modulates the expression of cellulase genes in Trichoderma reesei Rut-C30 via calcium signaling

    doi: 10.1186/s13068-018-1055-6

    Figure Lengend Snippet: Effects of Ca 2+ channel inhibitor LaCl 3 on the cytosolic Ca 2+ concentration and cellulase production. A Fluorescence analysis of LaCl 3 influence on cytosolic Ca 2+ burst induced by Mn 2+ . The T. reesei Rut-C30 were cultured in liquid minimal medium for 48–60 h with 0 or 10 mM MnCl 2 (0 or 10 Mn, respectively), and then treated with 0 or 5 mM LaCl 3 . For detection, 50 μM Fluo-3/AM was used, and the intensity was monitored using Automatic Inverted Fluorescence Microscopy. Green fluorescence represents the free cytosolic Ca 2+ . DIC, differential interference contrast, CK, not treated with LaCl 3 . B Comparative fluorescence ratio analysis of LaCl 3 influence on the cytosolic Ca 2+ burst induced by Mn 2+ . The y -axis represents the Ca 2+ fluorescence ratio measured by CLSM and the x- axis the different treatments. The CMCase activity ( C ) and p NPCase activity ( D ) of T. reesei Rut-C30 were examined after culture in medium containing 0 or 10 mM MnCl 2 and with (−) or without (+) 5 mM LaCl 3 . The expression levels of cbh1 ( E ) and egl1 ( F ) in T. reesei Rut-C30 were analyzed after culture in medium containing 0 or 10 mM MnCl 2 and with (−) or without (+) 5 mM LaCl 3 . Values are the mean ± SD of the results from three independent experiments. Different letters indicate significant differences between the columns ( p

    Article Snippet: Free cytosolic Ca2+ labeling and detection Fluo-3/AM (Sigma) was used as a Ca2+ -specific probe to assess the level of cytoplasmic Ca2+ in T. reesei Rut-C30 according to the manufacturer’s protocol.

    Techniques: Concentration Assay, Fluorescence, Cell Culture, Microscopy, Confocal Laser Scanning Microscopy, Activity Assay, Expressing

    Influence of TPMR1 on Mn 2+ -induced cytosolic Ca 2+ burst and cellulase production. A Fluorescence analysis of the influence of TPMR1 on the cytosolic Ca 2+ burst induced by Mn 2+ . The T. reesei Rut-C30 and Δ tpmr1 strains were cultured in liquid minimal medium for 48–60 h with 0 or 10 mM MnCl 2 (0 or 10 Mn, respectively). For detection, 50 μM Fluo-3/AM was used, and the intensity was monitored using Automatic Inverted Fluorescence Microscopy. Green fluorescence represents the free cytosolic Ca 2+ . DIC, differential interference contrast. B Comparative fluorescence ratio analysis of TPMR1 influence on cytosolic Ca 2+ burst induced by Mn 2+ . The y -axis represents the Ca 2+ fluorescence ratio measured by CLSM and the x -axis the different treatments. The CMCase activity ( C ) and p NPCase activity ( D ) of T. reesei Rut-C30 and Δ tpmr1 strains were examined after culture in medium containing 0 or 10 mM MnCl 2 . The expression levels of cbh1 ( E ) and egl1 ( F ) in T. reesei Rut-C30 and Δ tpmr1 strains were analyzed after culture in medium containing 0 or 10 mM MnCl 2 . Values are the mean ± SD of the results from three independent experiments. Asterisks indicate significant differences from untreated strains (* p

    Journal: Biotechnology for Biofuels

    Article Title: Mn2+ modulates the expression of cellulase genes in Trichoderma reesei Rut-C30 via calcium signaling

    doi: 10.1186/s13068-018-1055-6

    Figure Lengend Snippet: Influence of TPMR1 on Mn 2+ -induced cytosolic Ca 2+ burst and cellulase production. A Fluorescence analysis of the influence of TPMR1 on the cytosolic Ca 2+ burst induced by Mn 2+ . The T. reesei Rut-C30 and Δ tpmr1 strains were cultured in liquid minimal medium for 48–60 h with 0 or 10 mM MnCl 2 (0 or 10 Mn, respectively). For detection, 50 μM Fluo-3/AM was used, and the intensity was monitored using Automatic Inverted Fluorescence Microscopy. Green fluorescence represents the free cytosolic Ca 2+ . DIC, differential interference contrast. B Comparative fluorescence ratio analysis of TPMR1 influence on cytosolic Ca 2+ burst induced by Mn 2+ . The y -axis represents the Ca 2+ fluorescence ratio measured by CLSM and the x -axis the different treatments. The CMCase activity ( C ) and p NPCase activity ( D ) of T. reesei Rut-C30 and Δ tpmr1 strains were examined after culture in medium containing 0 or 10 mM MnCl 2 . The expression levels of cbh1 ( E ) and egl1 ( F ) in T. reesei Rut-C30 and Δ tpmr1 strains were analyzed after culture in medium containing 0 or 10 mM MnCl 2 . Values are the mean ± SD of the results from three independent experiments. Asterisks indicate significant differences from untreated strains (* p

    Article Snippet: Free cytosolic Ca2+ labeling and detection Fluo-3/AM (Sigma) was used as a Ca2+ -specific probe to assess the level of cytoplasmic Ca2+ in T. reesei Rut-C30 according to the manufacturer’s protocol.

    Techniques: Fluorescence, Cell Culture, Microscopy, Confocal Laser Scanning Microscopy, Activity Assay, Expressing

    Cytosolic Ca 2+ levels and calcium signaling increase after Mn 2+ addition. A The analysis of cytosolic Ca 2+ levels via a Ca 2+ fluorescent probe Fluo-3/AM. The T. reesei Rut-C30 and its derivative mutant strains were cultured in liquid minimal medium for 48–60 h with 0 or 10 mM final concentration of MnCl 2 (0 or 10 Mn, respectively). For detection, 50 μM Fluo-3/AM was used, and the intensity was monitored using Automatic Inverted Fluorescence Microscopy. Green fluorescence represents the free cytosolic Ca 2+ . DIC, differential interference contrast. B Comparative fluorescence ratio analysis of Mn 2+ influence on cytosolic Ca 2+ levels. The y -axis represents the Ca 2+ fluorescence ratio measured by CLSM and the x -axis the different strains tested. The effects of Mn 2+ on the expression levels of certain calcium signaling-related genes cam ( C ), cna1 ( D ) and crz1 ( E ) in T. reesei Rut-C30. 0 Mn, no MnCl 2 was added to the medium; 10 Mn, final concentration of 10 mM MnCl 2 . Values are the mean ± SD of the results from three independent experiments. Asterisks indicate significant differences from untreated strains (* p

    Journal: Biotechnology for Biofuels

    Article Title: Mn2+ modulates the expression of cellulase genes in Trichoderma reesei Rut-C30 via calcium signaling

    doi: 10.1186/s13068-018-1055-6

    Figure Lengend Snippet: Cytosolic Ca 2+ levels and calcium signaling increase after Mn 2+ addition. A The analysis of cytosolic Ca 2+ levels via a Ca 2+ fluorescent probe Fluo-3/AM. The T. reesei Rut-C30 and its derivative mutant strains were cultured in liquid minimal medium for 48–60 h with 0 or 10 mM final concentration of MnCl 2 (0 or 10 Mn, respectively). For detection, 50 μM Fluo-3/AM was used, and the intensity was monitored using Automatic Inverted Fluorescence Microscopy. Green fluorescence represents the free cytosolic Ca 2+ . DIC, differential interference contrast. B Comparative fluorescence ratio analysis of Mn 2+ influence on cytosolic Ca 2+ levels. The y -axis represents the Ca 2+ fluorescence ratio measured by CLSM and the x -axis the different strains tested. The effects of Mn 2+ on the expression levels of certain calcium signaling-related genes cam ( C ), cna1 ( D ) and crz1 ( E ) in T. reesei Rut-C30. 0 Mn, no MnCl 2 was added to the medium; 10 Mn, final concentration of 10 mM MnCl 2 . Values are the mean ± SD of the results from three independent experiments. Asterisks indicate significant differences from untreated strains (* p

    Article Snippet: Free cytosolic Ca2+ labeling and detection Fluo-3/AM (Sigma) was used as a Ca2+ -specific probe to assess the level of cytoplasmic Ca2+ in T. reesei Rut-C30 according to the manufacturer’s protocol.

    Techniques: Mutagenesis, Cell Culture, Concentration Assay, Fluorescence, Microscopy, Confocal Laser Scanning Microscopy, Expressing, Chick Chorioallantoic Membrane Assay

    Special indicator Fluo-3 AM was loading at 37 °C for 90 min in hepatocarcinoma cell lines in order to examine localization and concentration of intracellular calcium in MRC-1024 laser scanning confocal microscope imaging system. (A). In hepatocarcinoma cell line HHCC, slight signal of calcium with lower peak was observed. (B). Concentration of intracellular calcium in HHCC was 35.13 nmol/L.

    Journal: World Journal of Gastroenterology : WJG

    Article Title: Signal transduction of gap junctional genes, connexin32, connexin43 in human hepatocarcinogenesis

    doi: 10.3748/wjg.v9.i5.946

    Figure Lengend Snippet: Special indicator Fluo-3 AM was loading at 37 °C for 90 min in hepatocarcinoma cell lines in order to examine localization and concentration of intracellular calcium in MRC-1024 laser scanning confocal microscope imaging system. (A). In hepatocarcinoma cell line HHCC, slight signal of calcium with lower peak was observed. (B). Concentration of intracellular calcium in HHCC was 35.13 nmol/L.

    Article Snippet: Digested by trypsin and collected, HHCC, SMMC-7721 and QZG cells were loaded by 100 nmol/L special fluorescent dye indicator of calcium Fluo-3 AM (Sigma, USA) in serum free RPMI-1640 medium, 37 °C for 90 min, rinsed by Ca2+ free D-Hank’s for three times.

    Techniques: Concentration Assay, Microscopy, Imaging

    Special indicator Fluo-3 AM was loading at 37 °C for 90 min in hepatocarcinoma cell lines in order to examine localization and concentration of intracellular calcium in MRC-1024 laser scanning confocal microscope imaging system. (A). In normal hepatocyte cell line QZG, stronger signal of calcium with lower peak released. (B). Concentration of intracellular calcium in HHCC was 108.37 nmol/L.

    Journal: World Journal of Gastroenterology : WJG

    Article Title: Signal transduction of gap junctional genes, connexin32, connexin43 in human hepatocarcinogenesis

    doi: 10.3748/wjg.v9.i5.946

    Figure Lengend Snippet: Special indicator Fluo-3 AM was loading at 37 °C for 90 min in hepatocarcinoma cell lines in order to examine localization and concentration of intracellular calcium in MRC-1024 laser scanning confocal microscope imaging system. (A). In normal hepatocyte cell line QZG, stronger signal of calcium with lower peak released. (B). Concentration of intracellular calcium in HHCC was 108.37 nmol/L.

    Article Snippet: Digested by trypsin and collected, HHCC, SMMC-7721 and QZG cells were loaded by 100 nmol/L special fluorescent dye indicator of calcium Fluo-3 AM (Sigma, USA) in serum free RPMI-1640 medium, 37 °C for 90 min, rinsed by Ca2+ free D-Hank’s for three times.

    Techniques: Concentration Assay, Microscopy, Imaging

    Special indicator Fluo-3 AM was loading at 37 °C for 90 min in hepatocarcinoma cell lines in order to examine localizatio and concentration of intracellular calcium in MRC-1024 laser scanning confocal microscope imaging system. (A). Loading by Fluo-3 AM, dark signal of calcium and lower peak was also absent in hepatocarcinoma cell line SMMC-7721. (B). Concentration of intracellular calcium was 47.08 nmol/L.

    Journal: World Journal of Gastroenterology : WJG

    Article Title: Signal transduction of gap junctional genes, connexin32, connexin43 in human hepatocarcinogenesis

    doi: 10.3748/wjg.v9.i5.946

    Figure Lengend Snippet: Special indicator Fluo-3 AM was loading at 37 °C for 90 min in hepatocarcinoma cell lines in order to examine localizatio and concentration of intracellular calcium in MRC-1024 laser scanning confocal microscope imaging system. (A). Loading by Fluo-3 AM, dark signal of calcium and lower peak was also absent in hepatocarcinoma cell line SMMC-7721. (B). Concentration of intracellular calcium was 47.08 nmol/L.

    Article Snippet: Digested by trypsin and collected, HHCC, SMMC-7721 and QZG cells were loaded by 100 nmol/L special fluorescent dye indicator of calcium Fluo-3 AM (Sigma, USA) in serum free RPMI-1640 medium, 37 °C for 90 min, rinsed by Ca2+ free D-Hank’s for three times.

    Techniques: Concentration Assay, Microscopy, Imaging

    Effects of wortmannin and YD-3 on intracellular calcium mobilization in platelets. Fluo-3-loaded human platelets were incubated with DMSO, wortmannin (Wort, 200 nM) and/or YD-3 (20 µM) at 37°C for 5 min in the presence of 1 mM extracellular Ca 2+ , then thrombin (0.1 U·mL −1 ), PAR1-AP (20 µM) or PAR4-AP (100 µM) was added to trigger the increase of [Ca 2+ ] i . Results are representatives of four independent experiments and are quantified in the histograms. Values are mean ± SEM ( n = 4). * P

    Journal: British Journal of Pharmacology

    Article Title: The roles and mechanisms of PAR4 and P2Y12/phosphatidylinositol 3-kinase pathway in maintaining thrombin-induced platelet aggregation

    doi: 10.1111/j.1476-5381.2010.00921.x

    Figure Lengend Snippet: Effects of wortmannin and YD-3 on intracellular calcium mobilization in platelets. Fluo-3-loaded human platelets were incubated with DMSO, wortmannin (Wort, 200 nM) and/or YD-3 (20 µM) at 37°C for 5 min in the presence of 1 mM extracellular Ca 2+ , then thrombin (0.1 U·mL −1 ), PAR1-AP (20 µM) or PAR4-AP (100 µM) was added to trigger the increase of [Ca 2+ ] i . Results are representatives of four independent experiments and are quantified in the histograms. Values are mean ± SEM ( n = 4). * P

    Article Snippet: Bovine α-thrombin, wortmannin, 2-methylthioadenosine 5′-monophosphate triethylammonium salt (2Me-SAMP), 12-O-tetradecanoylphorbol 13-acetate (TPA), UCN-01 and fluo-3/AM were obtained from Sigma Chemical Co., St. Louis, MO, USA.

    Techniques: Incubation