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    Pfizer Inc fluconazole
    Response of experimental OPEC caused by <t>fluconazole-resistant</t> Candida albicans in immunocompromised rabbits to antifungal therapy measured by mean log concentration of organism in the stomach and duodenum. Data for untreated controls (control) ( n = 12), rabbits treated with PLD at 4 (PLD4; n = 8), 10 (PLD10; n = 8), 25 (PLD25; n = 8), and 50 mg/kg/day (PLD50; n = 8), rabbits treated with DAMB at 0.5 mg/kg/day (DAMB; n = 4), and rabbits treated with FLC at 2 mg/kg/day ( n = 10) are shown. Values are given as means ± SEM. Comparisons were to untreated controls by ANOVA with Dunn's correction for multiple comparisons or by the Mann-Whitney U test, as appropriate (*, P ≤ 0.05; †, P ≤ 0.01; ¶, P
    Fluconazole, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluconazole/product/Pfizer Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluconazole - by Bioz Stars, 2021-05
    86/100 stars
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    99
    Millipore fluconazole
    LC-MS analysis of sterols and lipids in WT and fen Δ/Δ cells. a LC-MS comparison of ergosterol and DMCDD levels in whole cell lysates and the plasma membrane fraction of WT (GZY803 and BWP17), erg3 Δ, fen1 Δ/Δ, and fen12 Δ/Δ cells with or without <t>fluconazole</t> treatment. Error bars represent s.d. from the mean of three independent experiments. Significance was measured using unpaired t -test (* p
    Fluconazole, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluconazole/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluconazole - by Bioz Stars, 2021-05
    99/100 stars
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    86
    Clinical and Laboratory Standards Institute fluconazole
    Disruption of Cas5 enhances <t>fluconazole</t> killing activity against C. albicans .
    Fluconazole, supplied by Clinical and Laboratory Standards Institute, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluconazole/product/Clinical and Laboratory Standards Institute
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluconazole - by Bioz Stars, 2021-05
    86/100 stars
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    86
    ATCC fluconazole
    Resistance to <t>fluconazole</t> can be induced in other species of Candida using flucytosine, 5-fluorouracil, and 5-fluorouridine.
    Fluconazole, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluconazole/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluconazole - by Bioz Stars, 2021-05
    86/100 stars
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    N/A
    Fluconazole is a triazole antifungal agent that is effective against most Candida strains In vivo fluconazole at 0 5 100 mg kg demonstrates protective activity in various experimental animal models
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    Response of experimental OPEC caused by fluconazole-resistant Candida albicans in immunocompromised rabbits to antifungal therapy measured by mean log concentration of organism in the stomach and duodenum. Data for untreated controls (control) ( n = 12), rabbits treated with PLD at 4 (PLD4; n = 8), 10 (PLD10; n = 8), 25 (PLD25; n = 8), and 50 mg/kg/day (PLD50; n = 8), rabbits treated with DAMB at 0.5 mg/kg/day (DAMB; n = 4), and rabbits treated with FLC at 2 mg/kg/day ( n = 10) are shown. Values are given as means ± SEM. Comparisons were to untreated controls by ANOVA with Dunn's correction for multiple comparisons or by the Mann-Whitney U test, as appropriate (*, P ≤ 0.05; †, P ≤ 0.01; ¶, P

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Efficacy of PLD-118, a Novel Inhibitor of Candida Isoleucyl-tRNA Synthetase, against Experimental Oropharyngeal and Esophageal Candidiasis Caused by Fluconazole-Resistant C. albicans

    doi: 10.1128/AAC.48.10.3959-3967.2004

    Figure Lengend Snippet: Response of experimental OPEC caused by fluconazole-resistant Candida albicans in immunocompromised rabbits to antifungal therapy measured by mean log concentration of organism in the stomach and duodenum. Data for untreated controls (control) ( n = 12), rabbits treated with PLD at 4 (PLD4; n = 8), 10 (PLD10; n = 8), 25 (PLD25; n = 8), and 50 mg/kg/day (PLD50; n = 8), rabbits treated with DAMB at 0.5 mg/kg/day (DAMB; n = 4), and rabbits treated with FLC at 2 mg/kg/day ( n = 10) are shown. Values are given as means ± SEM. Comparisons were to untreated controls by ANOVA with Dunn's correction for multiple comparisons or by the Mann-Whitney U test, as appropriate (*, P ≤ 0.05; †, P ≤ 0.01; ¶, P

    Article Snippet: To characterize the in vitro pharmacodynamics and potential fungicidal activity of PLD-118 (Pliva Pharmaceutical Industry, Inc., Zagreb, Croatia), time-kill assays were performed in the same fashion for all four C. albicans isolates, with fluconazole (Roerig-Pfizer, New York, N.Y.) used as a treatment control.

    Techniques: Concentration Assay, MANN-WHITNEY

    Response of experimental OPEC caused by fluconazole-resistant C. albicans in immunocompromised rabbits to antifungal therapy measured by mean log concentration of organism in the esophagus. Data for the following groups are shown: untreated controls (control) ( n = 12); rabbits treated with PLD at 4 (PLD4; n = 8), 10 (PLD10; n = 8), 25 (PLD25; n = 8), and 50 mg/kg/day (PLD50; n = 8); rabbits treated with DAMB at 0.5 mg/kg/day (DAMB; n = 4); and rabbits treated with FLC at 2 mg/kg/day ( n = 10) are shown. Values are given as means ± SEM. Comparisons were to untreated controls by ANOVA with Dunn's correction for multiple comparisons or by the Mann-Whitney U test, as appropriate (*, P ≤ 0.05; †, P ≤ 0.01; ¶, P

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Efficacy of PLD-118, a Novel Inhibitor of Candida Isoleucyl-tRNA Synthetase, against Experimental Oropharyngeal and Esophageal Candidiasis Caused by Fluconazole-Resistant C. albicans

    doi: 10.1128/AAC.48.10.3959-3967.2004

    Figure Lengend Snippet: Response of experimental OPEC caused by fluconazole-resistant C. albicans in immunocompromised rabbits to antifungal therapy measured by mean log concentration of organism in the esophagus. Data for the following groups are shown: untreated controls (control) ( n = 12); rabbits treated with PLD at 4 (PLD4; n = 8), 10 (PLD10; n = 8), 25 (PLD25; n = 8), and 50 mg/kg/day (PLD50; n = 8); rabbits treated with DAMB at 0.5 mg/kg/day (DAMB; n = 4); and rabbits treated with FLC at 2 mg/kg/day ( n = 10) are shown. Values are given as means ± SEM. Comparisons were to untreated controls by ANOVA with Dunn's correction for multiple comparisons or by the Mann-Whitney U test, as appropriate (*, P ≤ 0.05; †, P ≤ 0.01; ¶, P

    Article Snippet: To characterize the in vitro pharmacodynamics and potential fungicidal activity of PLD-118 (Pliva Pharmaceutical Industry, Inc., Zagreb, Croatia), time-kill assays were performed in the same fashion for all four C. albicans isolates, with fluconazole (Roerig-Pfizer, New York, N.Y.) used as a treatment control.

    Techniques: Concentration Assay, MANN-WHITNEY

    Time-kill curve plots of PLD-118 (PLD) and fluconazole (FLC) in YNB against C. albicans . Concentrations of PLD of 4, 8, 16, and 64 μg/ml and FLC of 128 μg/ml were studied in relation to a growth control. Data plotted are the means ± SEM (¶, P

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Efficacy of PLD-118, a Novel Inhibitor of Candida Isoleucyl-tRNA Synthetase, against Experimental Oropharyngeal and Esophageal Candidiasis Caused by Fluconazole-Resistant C. albicans

    doi: 10.1128/AAC.48.10.3959-3967.2004

    Figure Lengend Snippet: Time-kill curve plots of PLD-118 (PLD) and fluconazole (FLC) in YNB against C. albicans . Concentrations of PLD of 4, 8, 16, and 64 μg/ml and FLC of 128 μg/ml were studied in relation to a growth control. Data plotted are the means ± SEM (¶, P

    Article Snippet: To characterize the in vitro pharmacodynamics and potential fungicidal activity of PLD-118 (Pliva Pharmaceutical Industry, Inc., Zagreb, Croatia), time-kill assays were performed in the same fashion for all four C. albicans isolates, with fluconazole (Roerig-Pfizer, New York, N.Y.) used as a treatment control.

    Techniques:

    Response of experimental OPEC caused by fluconazole-resistant C. albicans in immunocompromised rabbits to antifungal therapy, as measured by mean log concentration of the organism in the tongue. Data for the following groups are shown: untreated controls (control; n = 12); rabbits treated with PLD-118 at 4 (PLD4; n = 8), 10 (PLD10; n = 8), 25 (PLD25; n = 8), and 50 mg/kg/day (PLD50; n = 8); rabbits treated with DAMB at 0.5 mg/kg/day (DAMB; n = 4); and rabbits treated with FLC at 2 mg/kg/day (FLC; n = 10). Values are given as means ± SEM. Comparisons were to untreated controls by ANOVA with Dunn's correction for multiple comparisons or by the Mann-Whitney U test, as appropriate (*, P ≤ 0.05; †, P ≤ 0.01; ¶, P

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Efficacy of PLD-118, a Novel Inhibitor of Candida Isoleucyl-tRNA Synthetase, against Experimental Oropharyngeal and Esophageal Candidiasis Caused by Fluconazole-Resistant C. albicans

    doi: 10.1128/AAC.48.10.3959-3967.2004

    Figure Lengend Snippet: Response of experimental OPEC caused by fluconazole-resistant C. albicans in immunocompromised rabbits to antifungal therapy, as measured by mean log concentration of the organism in the tongue. Data for the following groups are shown: untreated controls (control; n = 12); rabbits treated with PLD-118 at 4 (PLD4; n = 8), 10 (PLD10; n = 8), 25 (PLD25; n = 8), and 50 mg/kg/day (PLD50; n = 8); rabbits treated with DAMB at 0.5 mg/kg/day (DAMB; n = 4); and rabbits treated with FLC at 2 mg/kg/day (FLC; n = 10). Values are given as means ± SEM. Comparisons were to untreated controls by ANOVA with Dunn's correction for multiple comparisons or by the Mann-Whitney U test, as appropriate (*, P ≤ 0.05; †, P ≤ 0.01; ¶, P

    Article Snippet: To characterize the in vitro pharmacodynamics and potential fungicidal activity of PLD-118 (Pliva Pharmaceutical Industry, Inc., Zagreb, Croatia), time-kill assays were performed in the same fashion for all four C. albicans isolates, with fluconazole (Roerig-Pfizer, New York, N.Y.) used as a treatment control.

    Techniques: Concentration Assay, MANN-WHITNEY

    Response of experimental OPEC caused by fluconazole-resistant C. albicans in immunocompromised rabbits to antifungal therapy, as measured by mean log concentration of organism in the oropharynx. Data for the following groups are shown: untreated controls (control) ( n = 12); rabbits treated with PLD at 4 (PLD4; n = 8), 10 (PLD10; n = 8), 25 (PLD25; n = 8), and 50 mg/kg/day (PLD50; n = 8); rabbits treated with DAMB at 0.5 mg/kg/day (DAMB; n = 4); and rabbits treated with FLC at 2 mg/kg/day ( n = 10) are shown. Values are given as means ± SEM. Comparisons were to untreated controls by ANOVA with Dunn's correction for multiple comparisons or by the Mann-Whitney U test, as appropriate (*, P ≤ 0.05; †, P ≤ 0.01; ¶, P

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Efficacy of PLD-118, a Novel Inhibitor of Candida Isoleucyl-tRNA Synthetase, against Experimental Oropharyngeal and Esophageal Candidiasis Caused by Fluconazole-Resistant C. albicans

    doi: 10.1128/AAC.48.10.3959-3967.2004

    Figure Lengend Snippet: Response of experimental OPEC caused by fluconazole-resistant C. albicans in immunocompromised rabbits to antifungal therapy, as measured by mean log concentration of organism in the oropharynx. Data for the following groups are shown: untreated controls (control) ( n = 12); rabbits treated with PLD at 4 (PLD4; n = 8), 10 (PLD10; n = 8), 25 (PLD25; n = 8), and 50 mg/kg/day (PLD50; n = 8); rabbits treated with DAMB at 0.5 mg/kg/day (DAMB; n = 4); and rabbits treated with FLC at 2 mg/kg/day ( n = 10) are shown. Values are given as means ± SEM. Comparisons were to untreated controls by ANOVA with Dunn's correction for multiple comparisons or by the Mann-Whitney U test, as appropriate (*, P ≤ 0.05; †, P ≤ 0.01; ¶, P

    Article Snippet: To characterize the in vitro pharmacodynamics and potential fungicidal activity of PLD-118 (Pliva Pharmaceutical Industry, Inc., Zagreb, Croatia), time-kill assays were performed in the same fashion for all four C. albicans isolates, with fluconazole (Roerig-Pfizer, New York, N.Y.) used as a treatment control.

    Techniques: Concentration Assay, MANN-WHITNEY

    Antifungal effect of PLD-118 on cellular morphology of fluconazole-resistant C. albicans in experimental oropharyngeal and esophageal candidiasis. (A) Control, Grocott-Gomori methenamine-silver stain (GMS); (B) control, periodic acid-Schiff (PAS); (C) PLD10 group, GMS; (D) PLD10 group, PAS. Magnification (all panels), ×630.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Efficacy of PLD-118, a Novel Inhibitor of Candida Isoleucyl-tRNA Synthetase, against Experimental Oropharyngeal and Esophageal Candidiasis Caused by Fluconazole-Resistant C. albicans

    doi: 10.1128/AAC.48.10.3959-3967.2004

    Figure Lengend Snippet: Antifungal effect of PLD-118 on cellular morphology of fluconazole-resistant C. albicans in experimental oropharyngeal and esophageal candidiasis. (A) Control, Grocott-Gomori methenamine-silver stain (GMS); (B) control, periodic acid-Schiff (PAS); (C) PLD10 group, GMS; (D) PLD10 group, PAS. Magnification (all panels), ×630.

    Article Snippet: To characterize the in vitro pharmacodynamics and potential fungicidal activity of PLD-118 (Pliva Pharmaceutical Industry, Inc., Zagreb, Croatia), time-kill assays were performed in the same fashion for all four C. albicans isolates, with fluconazole (Roerig-Pfizer, New York, N.Y.) used as a treatment control.

    Techniques: Silver Staining

    LC-MS analysis of sterols and lipids in WT and fen Δ/Δ cells. a LC-MS comparison of ergosterol and DMCDD levels in whole cell lysates and the plasma membrane fraction of WT (GZY803 and BWP17), erg3 Δ, fen1 Δ/Δ, and fen12 Δ/Δ cells with or without fluconazole treatment. Error bars represent s.d. from the mean of three independent experiments. Significance was measured using unpaired t -test (* p

    Journal: Nature Communications

    Article Title: Candida albicans gains azole resistance by altering sphingolipid composition

    doi: 10.1038/s41467-018-06944-1

    Figure Lengend Snippet: LC-MS analysis of sterols and lipids in WT and fen Δ/Δ cells. a LC-MS comparison of ergosterol and DMCDD levels in whole cell lysates and the plasma membrane fraction of WT (GZY803 and BWP17), erg3 Δ, fen1 Δ/Δ, and fen12 Δ/Δ cells with or without fluconazole treatment. Error bars represent s.d. from the mean of three independent experiments. Significance was measured using unpaired t -test (* p

    Article Snippet: Stock solutions of fluconazole (Sigma, F8929-100MG) was prepared in dimethyl sulfoxide (Sigma, V900090-500ML) and stored at −20 °C until use.

    Techniques: Liquid Chromatography with Mass Spectroscopy

    Sphingolipid overproduction underlies fluconazole resistance of fen Δ/Δ cells. a Illustration of ergosterol and sphingolipid biosynthesis pathways. b qPCR analysis of UPC2 , ERG3 , ERG11 , LAG1 , AUR1 , MIT1 , and IPT1 expression in WT, fen1 Δ/Δ, and fen12 Δ/Δ mutants with UPC2 single or double knockout in the presence or absence of fluconazole. Error bars represent s.d. from the mean of three independent experiments. Significance was measured using unpaired t -test (* p

    Journal: Nature Communications

    Article Title: Candida albicans gains azole resistance by altering sphingolipid composition

    doi: 10.1038/s41467-018-06944-1

    Figure Lengend Snippet: Sphingolipid overproduction underlies fluconazole resistance of fen Δ/Δ cells. a Illustration of ergosterol and sphingolipid biosynthesis pathways. b qPCR analysis of UPC2 , ERG3 , ERG11 , LAG1 , AUR1 , MIT1 , and IPT1 expression in WT, fen1 Δ/Δ, and fen12 Δ/Δ mutants with UPC2 single or double knockout in the presence or absence of fluconazole. Error bars represent s.d. from the mean of three independent experiments. Significance was measured using unpaired t -test (* p

    Article Snippet: Stock solutions of fluconazole (Sigma, F8929-100MG) was prepared in dimethyl sulfoxide (Sigma, V900090-500ML) and stored at −20 °C until use.

    Techniques: Real-time Polymerase Chain Reaction, Expressing, Double Knockout

    Genetic screens for fluconazole-resistant mutants. a Volcano plot of fold changes of genes with PB inserts in PB transposition mutants grown in media containing 100 µg/mL fluconazole in comparison with the same mutants grown in normal media. p -Values were calculated using the Wald test. Ten candidates selected for phenotype confirmation are indicated. Genes known to be associated with fluconazole resistance and ergosterol and sphingolipid biosynthesis are shown with different colors. b Gene deletion of 10 candidates randomly selected from the top-ranked genes in a . Cultures were incubated for 24 h, and growth was measured as OD 600 and expressed as a percentage of the growth in the control wells with no drug (DMSO alone) for each strain. c Gene Ontology (GO) analysis of the PB -mutated genes that led to statistically significant fold increases ( p

    Journal: Nature Communications

    Article Title: Candida albicans gains azole resistance by altering sphingolipid composition

    doi: 10.1038/s41467-018-06944-1

    Figure Lengend Snippet: Genetic screens for fluconazole-resistant mutants. a Volcano plot of fold changes of genes with PB inserts in PB transposition mutants grown in media containing 100 µg/mL fluconazole in comparison with the same mutants grown in normal media. p -Values were calculated using the Wald test. Ten candidates selected for phenotype confirmation are indicated. Genes known to be associated with fluconazole resistance and ergosterol and sphingolipid biosynthesis are shown with different colors. b Gene deletion of 10 candidates randomly selected from the top-ranked genes in a . Cultures were incubated for 24 h, and growth was measured as OD 600 and expressed as a percentage of the growth in the control wells with no drug (DMSO alone) for each strain. c Gene Ontology (GO) analysis of the PB -mutated genes that led to statistically significant fold increases ( p

    Article Snippet: Stock solutions of fluconazole (Sigma, F8929-100MG) was prepared in dimethyl sulfoxide (Sigma, V900090-500ML) and stored at −20 °C until use.

    Techniques: Incubation

    Disruption of Cas5 enhances fluconazole killing activity against C. albicans .

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Disruption of the Transcriptional Regulator Cas5 Results in Enhanced Killing of Candida albicans by Fluconazole

    doi: 10.1128/AAC.00064-14

    Figure Lengend Snippet: Disruption of Cas5 enhances fluconazole killing activity against C. albicans .

    Article Snippet: The MICs of fluconazole were determined by using broth microdilution as described by the Clinical and Laboratory Standards Institute ( , ), modified by using both RPMI and YPD media, and were read both visually and spectrophotometrically at 24, 48, and 72 h for at least a 50% reduction in growth ( , ).

    Techniques: Activity Assay

    Effect of fluconazole on CAS5 in 12-99 as determined by time-kill assays. 12-99 or 12-99 cas5 Δ/Δ cells were diluted in YPD medium containing FLC (10 μg/ml) or the solvent dimethyl sulfoxide (DMSO). After 0, 6, 12, and 24 h, samples

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Disruption of the Transcriptional Regulator Cas5 Results in Enhanced Killing of Candida albicans by Fluconazole

    doi: 10.1128/AAC.00064-14

    Figure Lengend Snippet: Effect of fluconazole on CAS5 in 12-99 as determined by time-kill assays. 12-99 or 12-99 cas5 Δ/Δ cells were diluted in YPD medium containing FLC (10 μg/ml) or the solvent dimethyl sulfoxide (DMSO). After 0, 6, 12, and 24 h, samples

    Article Snippet: The MICs of fluconazole were determined by using broth microdilution as described by the Clinical and Laboratory Standards Institute ( , ), modified by using both RPMI and YPD media, and were read both visually and spectrophotometrically at 24, 48, and 72 h for at least a 50% reduction in growth ( , ).

    Techniques:

    Validation of fluconazole-inducible and Cas5-dependent gene expression. Expression levels of CFL4 , ERG26 , FTR1 , PGA13 , and PGA31 were measured in triplicate by qRT-PCR and compared to the expression levels in SC5314. Shown are the relative n -fold changes

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Disruption of the Transcriptional Regulator Cas5 Results in Enhanced Killing of Candida albicans by Fluconazole

    doi: 10.1128/AAC.00064-14

    Figure Lengend Snippet: Validation of fluconazole-inducible and Cas5-dependent gene expression. Expression levels of CFL4 , ERG26 , FTR1 , PGA13 , and PGA31 were measured in triplicate by qRT-PCR and compared to the expression levels in SC5314. Shown are the relative n -fold changes

    Article Snippet: The MICs of fluconazole were determined by using broth microdilution as described by the Clinical and Laboratory Standards Institute ( , ), modified by using both RPMI and YPD media, and were read both visually and spectrophotometrically at 24, 48, and 72 h for at least a 50% reduction in growth ( , ).

    Techniques: Expressing, Quantitative RT-PCR

    Disruption of Cas5 enhances fluconazole killing activity against C. albicans .

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Disruption of the Transcriptional Regulator Cas5 Results in Enhanced Killing of Candida albicans by Fluconazole

    doi: 10.1128/AAC.00064-14

    Figure Lengend Snippet: Disruption of Cas5 enhances fluconazole killing activity against C. albicans .

    Article Snippet: The MICs of fluconazole were determined by using broth microdilution as described by the Clinical and Laboratory Standards Institute ( , ), modified by using both RPMI and YPD media, and were read both visually and spectrophotometrically at 24, 48, and 72 h for at least a 50% reduction in growth ( , ).

    Techniques: Activity Assay

    Comparison of the gene expression profiles of wild-type strain SC5314 and the cas5 Δ/Δ mutant exposed to fluconazole.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Disruption of the Transcriptional Regulator Cas5 Results in Enhanced Killing of Candida albicans by Fluconazole

    doi: 10.1128/AAC.00064-14

    Figure Lengend Snippet: Comparison of the gene expression profiles of wild-type strain SC5314 and the cas5 Δ/Δ mutant exposed to fluconazole.

    Article Snippet: The MICs of fluconazole were determined by using broth microdilution as described by the Clinical and Laboratory Standards Institute ( , ), modified by using both RPMI and YPD media, and were read both visually and spectrophotometrically at 24, 48, and 72 h for at least a 50% reduction in growth ( , ).

    Techniques: Expressing, Mutagenesis

    Disruption of CAS5 in strains containing resistance mutations in MRR1 , TAC1 , and ERG11 impacts fluconazole susceptibility.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Disruption of the Transcriptional Regulator Cas5 Results in Enhanced Killing of Candida albicans by Fluconazole

    doi: 10.1128/AAC.00064-14

    Figure Lengend Snippet: Disruption of CAS5 in strains containing resistance mutations in MRR1 , TAC1 , and ERG11 impacts fluconazole susceptibility.

    Article Snippet: The MICs of fluconazole were determined by using broth microdilution as described by the Clinical and Laboratory Standards Institute ( , ), modified by using both RPMI and YPD media, and were read both visually and spectrophotometrically at 24, 48, and 72 h for at least a 50% reduction in growth ( , ).

    Techniques:

    Resistance to fluconazole can be induced in other species of Candida using flucytosine, 5-fluorouracil, and 5-fluorouridine.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Deletion of the Uracil Permease Gene Confers Cross-Resistance to 5-Fluorouracil and Azoles in Candida lusitaniae and Highlights Antagonistic Interaction between Fluorinated Nucleotides and Fluconazole

    doi: 10.1128/AAC.00009-14

    Figure Lengend Snippet: Resistance to fluconazole can be induced in other species of Candida using flucytosine, 5-fluorouracil, and 5-fluorouridine.

    Article Snippet: First, the MICs of fluoropyrimidines and fluconazole were determined for a representative panel of strains of different species, including the strain ATCC 42720 of C. lusitaniae , used as a control ( ).

    Techniques:

    Etest determination of the fluconazole MIC of the C. lusitaniae fur4 Δ and fur1 Δ mutant strains on RPMI medium supplemented with increasing concentrations of 5FU or left unsupplemented.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Deletion of the Uracil Permease Gene Confers Cross-Resistance to 5-Fluorouracil and Azoles in Candida lusitaniae and Highlights Antagonistic Interaction between Fluorinated Nucleotides and Fluconazole

    doi: 10.1128/AAC.00009-14

    Figure Lengend Snippet: Etest determination of the fluconazole MIC of the C. lusitaniae fur4 Δ and fur1 Δ mutant strains on RPMI medium supplemented with increasing concentrations of 5FU or left unsupplemented.

    Article Snippet: First, the MICs of fluoropyrimidines and fluconazole were determined for a representative panel of strains of different species, including the strain ATCC 42720 of C. lusitaniae , used as a control ( ).

    Techniques: Mutagenesis

    Growth of the antifungal-susceptible wild-type strain 6936 in RPMI supplemented with inhibitory concentrations of fluconazole along with various low concentrations of 5-fluorouracil (A) or flucytosine (B). Growth is given as a percentage of the growth

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Deletion of the Uracil Permease Gene Confers Cross-Resistance to 5-Fluorouracil and Azoles in Candida lusitaniae and Highlights Antagonistic Interaction between Fluorinated Nucleotides and Fluconazole

    doi: 10.1128/AAC.00009-14

    Figure Lengend Snippet: Growth of the antifungal-susceptible wild-type strain 6936 in RPMI supplemented with inhibitory concentrations of fluconazole along with various low concentrations of 5-fluorouracil (A) or flucytosine (B). Growth is given as a percentage of the growth

    Article Snippet: First, the MICs of fluoropyrimidines and fluconazole were determined for a representative panel of strains of different species, including the strain ATCC 42720 of C. lusitaniae , used as a control ( ).

    Techniques:

    Growth of C. guilliermondii ATCC 6260, C. glabrata ATCC 90030, and C. tropicalis IP1275 in RPMI medium supplemented with inhibitory concentrations of fluconazole (4× the MIC for each strain) in the presence of different concentrations of the fluoropyrimidines

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Deletion of the Uracil Permease Gene Confers Cross-Resistance to 5-Fluorouracil and Azoles in Candida lusitaniae and Highlights Antagonistic Interaction between Fluorinated Nucleotides and Fluconazole

    doi: 10.1128/AAC.00009-14

    Figure Lengend Snippet: Growth of C. guilliermondii ATCC 6260, C. glabrata ATCC 90030, and C. tropicalis IP1275 in RPMI medium supplemented with inhibitory concentrations of fluconazole (4× the MIC for each strain) in the presence of different concentrations of the fluoropyrimidines

    Article Snippet: First, the MICs of fluoropyrimidines and fluconazole were determined for a representative panel of strains of different species, including the strain ATCC 42720 of C. lusitaniae , used as a control ( ).

    Techniques:

    Growth of wild-type and ura3 Δ strains of C. lusitaniae in RMPI supplemented with 8 μg/ml fluconazole and different concentrations of 5FOA. Growth is expressed as the percentage of growth observed in inhibitor-free RPMI. The lack of bars

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Deletion of the Uracil Permease Gene Confers Cross-Resistance to 5-Fluorouracil and Azoles in Candida lusitaniae and Highlights Antagonistic Interaction between Fluorinated Nucleotides and Fluconazole

    doi: 10.1128/AAC.00009-14

    Figure Lengend Snippet: Growth of wild-type and ura3 Δ strains of C. lusitaniae in RMPI supplemented with 8 μg/ml fluconazole and different concentrations of 5FOA. Growth is expressed as the percentage of growth observed in inhibitor-free RPMI. The lack of bars

    Article Snippet: First, the MICs of fluoropyrimidines and fluconazole were determined for a representative panel of strains of different species, including the strain ATCC 42720 of C. lusitaniae , used as a control ( ).

    Techniques:

    Resistance to fluconazole can be induced in other species of Candida using flucytosine, 5-fluorouracil, and 5-fluorouridine.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Deletion of the Uracil Permease Gene Confers Cross-Resistance to 5-Fluorouracil and Azoles in Candida lusitaniae and Highlights Antagonistic Interaction between Fluorinated Nucleotides and Fluconazole

    doi: 10.1128/AAC.00009-14

    Figure Lengend Snippet: Resistance to fluconazole can be induced in other species of Candida using flucytosine, 5-fluorouracil, and 5-fluorouridine.

    Article Snippet: First, the MICs of fluoropyrimidines and fluconazole were determined for a representative panel of strains of different species, including the strain ATCC 42720 of C. lusitaniae , used as a control ( ).

    Techniques: