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Image Search Results
Journal: BMC Veterinary Research
Article Title: Long-term changes of Th17 and regulatory T cells in peripheral blood of dogs with spinal cord injury after intervertebral disc herniation
doi: 10.1186/s12917-023-03647-8
Figure Lengend Snippet: Illustration of a Treg and a Th17 cell analysis using flow cytometry Results of Treg and Th17 cell analysis of a dog after recovery of spinal cord injury using MACSQuant analysis software In the prior performed isotype controls, limits were set at approx. 2% of the cells [ , – ]. This limit was transferred to the test fractions (pink lines) in each case to detect the percentages of FOXP3 (a) or IL17 (b) positive cells, which are located to the right of the pink line Absolute cell numbers were subsequently calculated to make the analyses comparable a) The graphic displays the percentage of FOXP3 positive cells for detection of Treg cells. 9.56% FOXP3 positive cells were identified b) The graphic displays the percentage of IL17 positive cells for detection of Th17. 16.20% IL17 positive cells were identified. The results of a stimulated Th17 cell fraction are shown
Article Snippet: For cell fixation, a
Techniques: Cell Analysis, Flow Cytometry, Software
Journal: International Journal of Molecular Sciences
Article Title: Reduced Dendritic Cells Expressing CD200R1 in Children with Inflammatory Bowel Disease: Correlation with Th17 and Regulatory T Cells
doi: 10.3390/ijms161226143
Figure Lengend Snippet: Representative flow cytometry data to illustrate the gating strategy for FACS analysis of DC subsets. ( A ) Dendritic cell HLA-DR + Lin − cells were gated ( R1 ), and myeloid CD11c + mDCs ( R2 ), plasmacytoid CD123 + pDCs ( R3 ) were determined as a percentage of the total PBMC ( B ). Dot plots to illustrate the expression of CD200 and CD200R1 on the myeloid dendritic cell (mDC) subset of healthy controls and IBD patients ( C , D ) and on and plasmacytoid DCs (pDCs) of healthy controls and IBD patients ( E , F ).
Article Snippet: For regulatory T cell staining, 1 × 10 6 cells were resuspended in 100 μL
Techniques: Flow Cytometry, Expressing