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    Thermo Fisher flow cytometry
    Flow Cytometry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Beckman Coulter flow cytometry
    Flow Cytometry, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson flow cytometry
    Flow Cytometry, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flow cytometry/product/Becton Dickinson
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    Becton Dickinson flow cytometric analysis
    Proteasome inhibition with ixazomib results in similar levels of tumor cell apoptosis compared with bortezomib in human melanoma cell lines BRAF V600E mutant (A375) and BRAF wild-type (WM1366) human melanoma tumor cells plated at a density of 2 × 10 5 cells/well on 6 well plates were treated for 24 or 48 hours with complete medium supplemented with either 35 nM ixazomib or 10 nM bortezomib. After incubation, the cells were subjected to annexin V/propidium iodide staining and flow <t>cytometric</t> analysis to determine the levels of apoptosis.
    Flow Cytometric Analysis, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flow cytometric analysis/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
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    flow cytometric analysis - by Bioz Stars, 2021-04
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    The 2 day basic flow cytometry training course conveys all necessary information enabling participants to successfully perform flow cytometry experiments This course is recommended for any person interested in using
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    Cayman s LDL Uptake Flow Cytometry Assay Kit employs human LDL conjugated to DyLight 488 as a convenient tool for studying the uptake of LDL in cultured cells Flow cytometry
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    APO BRDU FLOW CYTOMETRY KIT APO BRDU FLOW CYTOMETRY KIT
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    Image Search Results


    Proteasome inhibition with ixazomib results in similar levels of tumor cell apoptosis compared with bortezomib in human melanoma cell lines BRAF V600E mutant (A375) and BRAF wild-type (WM1366) human melanoma tumor cells plated at a density of 2 × 10 5 cells/well on 6 well plates were treated for 24 or 48 hours with complete medium supplemented with either 35 nM ixazomib or 10 nM bortezomib. After incubation, the cells were subjected to annexin V/propidium iodide staining and flow cytometric analysis to determine the levels of apoptosis.

    Journal: Oncotarget

    Article Title: The combination of MLN2238 (ixazomib) with interferon-alpha results in enhanced cell death in melanoma

    doi: 10.18632/oncotarget.12791

    Figure Lengend Snippet: Proteasome inhibition with ixazomib results in similar levels of tumor cell apoptosis compared with bortezomib in human melanoma cell lines BRAF V600E mutant (A375) and BRAF wild-type (WM1366) human melanoma tumor cells plated at a density of 2 × 10 5 cells/well on 6 well plates were treated for 24 or 48 hours with complete medium supplemented with either 35 nM ixazomib or 10 nM bortezomib. After incubation, the cells were subjected to annexin V/propidium iodide staining and flow cytometric analysis to determine the levels of apoptosis.

    Article Snippet: Analysis of apoptosis via annexin V/Propidium Iodine (PI) staining Apoptosis-induced phosphatidyl serine exposure was measured in tumor cells by flow cytometric analysis on an LSR II flow cytometer (BD Pharmingen, San Jose, CA) using APC-conjugated anti-annexin V and PE-conjugated anti-propidium iodide (BD Pharmingen, San Jose, CA) as previously described [ ].

    Techniques: Inhibition, Mutagenesis, Incubation, Staining, Flow Cytometry

    Ixazomib enhances the apoptotic effects of other anti-tumor agents BRAF V600E mutant (A375) human melanoma tumor cells plated at a density of 2 × 10 5 cells/well on a 6 well plate were treated for 48 hours with 35 nM ixazomib in combination with 100 ng/mL IL-29, 1 μM PLX4720, or 5 μM sorafenib and evaluated for levels of apoptosis via annexin V/propidium iodide staining and flow cytometric analysis. Data represented as mean ± standard error of the mean.

    Journal: Oncotarget

    Article Title: The combination of MLN2238 (ixazomib) with interferon-alpha results in enhanced cell death in melanoma

    doi: 10.18632/oncotarget.12791

    Figure Lengend Snippet: Ixazomib enhances the apoptotic effects of other anti-tumor agents BRAF V600E mutant (A375) human melanoma tumor cells plated at a density of 2 × 10 5 cells/well on a 6 well plate were treated for 48 hours with 35 nM ixazomib in combination with 100 ng/mL IL-29, 1 μM PLX4720, or 5 μM sorafenib and evaluated for levels of apoptosis via annexin V/propidium iodide staining and flow cytometric analysis. Data represented as mean ± standard error of the mean.

    Article Snippet: Analysis of apoptosis via annexin V/Propidium Iodine (PI) staining Apoptosis-induced phosphatidyl serine exposure was measured in tumor cells by flow cytometric analysis on an LSR II flow cytometer (BD Pharmingen, San Jose, CA) using APC-conjugated anti-annexin V and PE-conjugated anti-propidium iodide (BD Pharmingen, San Jose, CA) as previously described [ ].

    Techniques: Mutagenesis, Staining, Flow Cytometry

    Treatment of BRAF V600E mutant human melanoma cell lines with ixazomib leads to apoptotic cell death with half maximal effective concentration (EC 50 ) of 28.8 nM A375 (BRAF V600E mutant) human melanoma tumor cells were plated on a 6 well plate at a density of 2 × 10 5 cells/well. A. A375 cells were treated with complete medium supplemented with either 10% dimethyl sulfoxide (control) or with 65 nM ixazomib. Differential interference contrast images were obtained using an Olympus Fluoview 1000MPE confocal microscope after 48 hour incubation. B. A375 cells were treated with complete medium supplemented with either 10% dimethyl sulfoxide (control, 0 nM ixazomib dose) or with varying doses of ixazomib for 48 hours. After incubation, the cells were subjected to annexin V/propidium iodide staining and flow cytometric analysis to determine the levels of apoptosis. Data represented as mean ± standard error of the mean.

    Journal: Oncotarget

    Article Title: The combination of MLN2238 (ixazomib) with interferon-alpha results in enhanced cell death in melanoma

    doi: 10.18632/oncotarget.12791

    Figure Lengend Snippet: Treatment of BRAF V600E mutant human melanoma cell lines with ixazomib leads to apoptotic cell death with half maximal effective concentration (EC 50 ) of 28.8 nM A375 (BRAF V600E mutant) human melanoma tumor cells were plated on a 6 well plate at a density of 2 × 10 5 cells/well. A. A375 cells were treated with complete medium supplemented with either 10% dimethyl sulfoxide (control) or with 65 nM ixazomib. Differential interference contrast images were obtained using an Olympus Fluoview 1000MPE confocal microscope after 48 hour incubation. B. A375 cells were treated with complete medium supplemented with either 10% dimethyl sulfoxide (control, 0 nM ixazomib dose) or with varying doses of ixazomib for 48 hours. After incubation, the cells were subjected to annexin V/propidium iodide staining and flow cytometric analysis to determine the levels of apoptosis. Data represented as mean ± standard error of the mean.

    Article Snippet: Analysis of apoptosis via annexin V/Propidium Iodine (PI) staining Apoptosis-induced phosphatidyl serine exposure was measured in tumor cells by flow cytometric analysis on an LSR II flow cytometer (BD Pharmingen, San Jose, CA) using APC-conjugated anti-annexin V and PE-conjugated anti-propidium iodide (BD Pharmingen, San Jose, CA) as previously described [ ].

    Techniques: Mutagenesis, Concentration Assay, Microscopy, Incubation, Staining, Flow Cytometry

    Treatment of BRAF wild-type human melanoma cell lines with ixazomib leads to apoptotic cell death with half maximal effective concentration (EC 50 ) of 26.9 nM WM1366 (BRAF wild-type) human melanoma tumor cells were plated on a 6 well plate at a density of 2 × 10 5 cells/well. A. WM1366 cells were treated for 48 hours with complete medium supplemented with either 10% dimethyl sulfoxide (control) or with 35 nM ixazomib. Cells were then subjected to annexin V/propidium iodide (PI) staining and flow cytometric analysis to determine the levels of apoptosis. B. WM1366 cells were treated for 48 hours with complete medium supplemented with either 10% dimethyl sulfoxide (control, 0 nM ixazomib dose) or with varying doses of ixazomib. After incubation, the cells were subjected to annexin V/PI staining and flow cytometric analysis to determine the levels of apoptosis. Data represented as mean ± standard error of the mean.

    Journal: Oncotarget

    Article Title: The combination of MLN2238 (ixazomib) with interferon-alpha results in enhanced cell death in melanoma

    doi: 10.18632/oncotarget.12791

    Figure Lengend Snippet: Treatment of BRAF wild-type human melanoma cell lines with ixazomib leads to apoptotic cell death with half maximal effective concentration (EC 50 ) of 26.9 nM WM1366 (BRAF wild-type) human melanoma tumor cells were plated on a 6 well plate at a density of 2 × 10 5 cells/well. A. WM1366 cells were treated for 48 hours with complete medium supplemented with either 10% dimethyl sulfoxide (control) or with 35 nM ixazomib. Cells were then subjected to annexin V/propidium iodide (PI) staining and flow cytometric analysis to determine the levels of apoptosis. B. WM1366 cells were treated for 48 hours with complete medium supplemented with either 10% dimethyl sulfoxide (control, 0 nM ixazomib dose) or with varying doses of ixazomib. After incubation, the cells were subjected to annexin V/PI staining and flow cytometric analysis to determine the levels of apoptosis. Data represented as mean ± standard error of the mean.

    Article Snippet: Analysis of apoptosis via annexin V/Propidium Iodine (PI) staining Apoptosis-induced phosphatidyl serine exposure was measured in tumor cells by flow cytometric analysis on an LSR II flow cytometer (BD Pharmingen, San Jose, CA) using APC-conjugated anti-annexin V and PE-conjugated anti-propidium iodide (BD Pharmingen, San Jose, CA) as previously described [ ].

    Techniques: Concentration Assay, Staining, Flow Cytometry, Incubation

    Addition of IFN-α to treatment of melanoma cell lines with proteasome inhibitor results in enhanced tumor cell apoptosis BRAF V600E mutant (A375) and BRAF wild-type (WM1366) human melanoma cells plated at a density of 2 × 10 5 cells/well on 6 well plates were treated for 48 hours with complete medium supplemented with either 5 × 10 3 U/mL IFN-α, 35 nM ixazomib, 10 nM bortezomib, or with the combination of ixazomib or bortezomib plus IFN-α. After 48 hour incubation, the cells were subjected to annexin V/propidium iodide staining and flow cytometric analysis to determine the levels of apoptosis.

    Journal: Oncotarget

    Article Title: The combination of MLN2238 (ixazomib) with interferon-alpha results in enhanced cell death in melanoma

    doi: 10.18632/oncotarget.12791

    Figure Lengend Snippet: Addition of IFN-α to treatment of melanoma cell lines with proteasome inhibitor results in enhanced tumor cell apoptosis BRAF V600E mutant (A375) and BRAF wild-type (WM1366) human melanoma cells plated at a density of 2 × 10 5 cells/well on 6 well plates were treated for 48 hours with complete medium supplemented with either 5 × 10 3 U/mL IFN-α, 35 nM ixazomib, 10 nM bortezomib, or with the combination of ixazomib or bortezomib plus IFN-α. After 48 hour incubation, the cells were subjected to annexin V/propidium iodide staining and flow cytometric analysis to determine the levels of apoptosis.

    Article Snippet: Analysis of apoptosis via annexin V/Propidium Iodine (PI) staining Apoptosis-induced phosphatidyl serine exposure was measured in tumor cells by flow cytometric analysis on an LSR II flow cytometer (BD Pharmingen, San Jose, CA) using APC-conjugated anti-annexin V and PE-conjugated anti-propidium iodide (BD Pharmingen, San Jose, CA) as previously described [ ].

    Techniques: Mutagenesis, Incubation, Staining, Flow Cytometry

    Ixazomib induces apoptosis in human BRAF V600E mutant (A375) cell line with maximal levels reached at 48 hours A375 (BRAF V600E mutant) human melanoma tumor cells plated at a density of 2 × 10 5 cells/well on a 6 well plate were treated with complete medium supplemented with either 10% dimethyl sulfoxide (control) or 35 nM ixazomib. Cells were incubated for 12, 24 or 48 hours. The cells were subjected to annexin V/propidium iodide staining and flow cytometric analysis to determine levels of apoptosis.

    Journal: Oncotarget

    Article Title: The combination of MLN2238 (ixazomib) with interferon-alpha results in enhanced cell death in melanoma

    doi: 10.18632/oncotarget.12791

    Figure Lengend Snippet: Ixazomib induces apoptosis in human BRAF V600E mutant (A375) cell line with maximal levels reached at 48 hours A375 (BRAF V600E mutant) human melanoma tumor cells plated at a density of 2 × 10 5 cells/well on a 6 well plate were treated with complete medium supplemented with either 10% dimethyl sulfoxide (control) or 35 nM ixazomib. Cells were incubated for 12, 24 or 48 hours. The cells were subjected to annexin V/propidium iodide staining and flow cytometric analysis to determine levels of apoptosis.

    Article Snippet: Analysis of apoptosis via annexin V/Propidium Iodine (PI) staining Apoptosis-induced phosphatidyl serine exposure was measured in tumor cells by flow cytometric analysis on an LSR II flow cytometer (BD Pharmingen, San Jose, CA) using APC-conjugated anti-annexin V and PE-conjugated anti-propidium iodide (BD Pharmingen, San Jose, CA) as previously described [ ].

    Techniques: Mutagenesis, Incubation, Staining, Flow Cytometry