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    • flexstation 3  (Molecular Devices LLC)
    92
    Molecular Devices LLC flexstation 3
    Intracellular calcium flux and single-cell contractility evoked by 20 volatile odorants. ( A ) Odorant-evoked [Ca 2+ ] i was measured using FLIPR Calcium 5 assay. For each well of human ASM cells (∼30,000 cells/well), baseline Calcium 5 dye fluorescence was measured for the first 15 s, and after odorant addition (t = 15 s), the fluorescent reading was continuously measured using a FlexStation 3. For vehicle control, [Ca 2+ ] i was measured in response to 0.1% DMSO. As positive control, cells were stimulated with 10 μM histamine. Data are presented as mean ± SE ( n = 3 independent measurements). ( B ) Dynamic changes in the stiffness of human ASM cells were measured in response to each odorant molecule using OMTC. For each individual human ASM cell, baseline stiffness was measured for the first 60 s, and after odorant addition (t = 60 s), stiffness was continuously measured for the next 240 s. For each cell, stiffness was normalized to its baseline stiffness prior to odorant stimulation (normalized baseline is indicated by dotted line). For vehicle control, stiffness was measured in response to 0.1% DMSO. As positive controls, cell were contracted with 10 μM histamine and relaxed with 10 μM isoproterenol. Data are presented as mean ± SE ( n = 92 to 403 individual cell measurements). The colors indicated the odor types of 20 volatile chemicals (putrid, nutty, fruity, floral, caramellic, herbaceous, and minty); the concentrations used for each odorant molecule are shown in SI Appendix , Table S1 .
    Flexstation 3, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 92/100, based on 1648 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flexstation 3/product/Molecular Devices LLC
    Average 92 stars, based on 1648 article reviews
    Price from $9.99 to $1999.99
    flexstation 3 - by Bioz Stars, 2021-01
    92/100 stars
    		  Buy from Supplier
    flexstation 3 microplate reader  (Molecular Devices LLC)
    92
    Molecular Devices LLC flexstation 3 microplate reader
    Intracellular calcium flux and single-cell contractility evoked by 20 volatile odorants. ( A ) Odorant-evoked [Ca 2+ ] i was measured using FLIPR Calcium 5 assay. For each well of human ASM cells (∼30,000 cells/well), baseline Calcium 5 dye fluorescence was measured for the first 15 s, and after odorant addition (t = 15 s), the fluorescent reading was continuously measured using a FlexStation 3. For vehicle control, [Ca 2+ ] i was measured in response to 0.1% DMSO. As positive control, cells were stimulated with 10 μM histamine. Data are presented as mean ± SE ( n = 3 independent measurements). ( B ) Dynamic changes in the stiffness of human ASM cells were measured in response to each odorant molecule using OMTC. For each individual human ASM cell, baseline stiffness was measured for the first 60 s, and after odorant addition (t = 60 s), stiffness was continuously measured for the next 240 s. For each cell, stiffness was normalized to its baseline stiffness prior to odorant stimulation (normalized baseline is indicated by dotted line). For vehicle control, stiffness was measured in response to 0.1% DMSO. As positive controls, cell were contracted with 10 μM histamine and relaxed with 10 μM isoproterenol. Data are presented as mean ± SE ( n = 92 to 403 individual cell measurements). The colors indicated the odor types of 20 volatile chemicals (putrid, nutty, fruity, floral, caramellic, herbaceous, and minty); the concentrations used for each odorant molecule are shown in SI Appendix , Table S1 .
    Flexstation 3 Microplate Reader, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 92/100, based on 1378 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flexstation 3 microplate reader/product/Molecular Devices LLC
    Average 92 stars, based on 1378 article reviews
    Price from $9.99 to $1999.99
    flexstation 3 microplate reader - by Bioz Stars, 2021-01
    92/100 stars
    		  Buy from Supplier
    flexstation 3 plate reader  (Molecular Devices LLC)
    91
    Molecular Devices LLC flexstation 3 plate reader
    Intracellular calcium flux and single-cell contractility evoked by 20 volatile odorants. ( A ) Odorant-evoked [Ca 2+ ] i was measured using FLIPR Calcium 5 assay. For each well of human ASM cells (∼30,000 cells/well), baseline Calcium 5 dye fluorescence was measured for the first 15 s, and after odorant addition (t = 15 s), the fluorescent reading was continuously measured using a FlexStation 3. For vehicle control, [Ca 2+ ] i was measured in response to 0.1% DMSO. As positive control, cells were stimulated with 10 μM histamine. Data are presented as mean ± SE ( n = 3 independent measurements). ( B ) Dynamic changes in the stiffness of human ASM cells were measured in response to each odorant molecule using OMTC. For each individual human ASM cell, baseline stiffness was measured for the first 60 s, and after odorant addition (t = 60 s), stiffness was continuously measured for the next 240 s. For each cell, stiffness was normalized to its baseline stiffness prior to odorant stimulation (normalized baseline is indicated by dotted line). For vehicle control, stiffness was measured in response to 0.1% DMSO. As positive controls, cell were contracted with 10 μM histamine and relaxed with 10 μM isoproterenol. Data are presented as mean ± SE ( n = 92 to 403 individual cell measurements). The colors indicated the odor types of 20 volatile chemicals (putrid, nutty, fruity, floral, caramellic, herbaceous, and minty); the concentrations used for each odorant molecule are shown in SI Appendix , Table S1 .
    Flexstation 3 Plate Reader, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 91/100, based on 461 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flexstation 3 plate reader/product/Molecular Devices LLC
    Average 91 stars, based on 461 article reviews
    Price from $9.99 to $1999.99
    flexstation 3 plate reader - by Bioz Stars, 2021-01
    91/100 stars
    		  Buy from Supplier
    flexstation  (Molecular Devices LLC)
    91
    Molecular Devices LLC flexstation
    Intracellular calcium flux and single-cell contractility evoked by 20 volatile odorants. ( A ) Odorant-evoked [Ca 2+ ] i was measured using FLIPR Calcium 5 assay. For each well of human ASM cells (∼30,000 cells/well), baseline Calcium 5 dye fluorescence was measured for the first 15 s, and after odorant addition (t = 15 s), the fluorescent reading was continuously measured using a FlexStation 3. For vehicle control, [Ca 2+ ] i was measured in response to 0.1% DMSO. As positive control, cells were stimulated with 10 μM histamine. Data are presented as mean ± SE ( n = 3 independent measurements). ( B ) Dynamic changes in the stiffness of human ASM cells were measured in response to each odorant molecule using OMTC. For each individual human ASM cell, baseline stiffness was measured for the first 60 s, and after odorant addition (t = 60 s), stiffness was continuously measured for the next 240 s. For each cell, stiffness was normalized to its baseline stiffness prior to odorant stimulation (normalized baseline is indicated by dotted line). For vehicle control, stiffness was measured in response to 0.1% DMSO. As positive controls, cell were contracted with 10 μM histamine and relaxed with 10 μM isoproterenol. Data are presented as mean ± SE ( n = 92 to 403 individual cell measurements). The colors indicated the odor types of 20 volatile chemicals (putrid, nutty, fruity, floral, caramellic, herbaceous, and minty); the concentrations used for each odorant molecule are shown in SI Appendix , Table S1 .
    Flexstation, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 91/100, based on 698 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flexstation/product/Molecular Devices LLC
    Average 91 stars, based on 698 article reviews
    Price from $9.99 to $1999.99
    flexstation - by Bioz Stars, 2021-01
    91/100 stars
    		  Buy from Supplier
    flexstation ii 384  (Molecular Devices LLC)
    97
    Molecular Devices LLC flexstation ii 384
    Intracellular calcium flux and single-cell contractility evoked by 20 volatile odorants. ( A ) Odorant-evoked [Ca 2+ ] i was measured using FLIPR Calcium 5 assay. For each well of human ASM cells (∼30,000 cells/well), baseline Calcium 5 dye fluorescence was measured for the first 15 s, and after odorant addition (t = 15 s), the fluorescent reading was continuously measured using a FlexStation 3. For vehicle control, [Ca 2+ ] i was measured in response to 0.1% DMSO. As positive control, cells were stimulated with 10 μM histamine. Data are presented as mean ± SE ( n = 3 independent measurements). ( B ) Dynamic changes in the stiffness of human ASM cells were measured in response to each odorant molecule using OMTC. For each individual human ASM cell, baseline stiffness was measured for the first 60 s, and after odorant addition (t = 60 s), stiffness was continuously measured for the next 240 s. For each cell, stiffness was normalized to its baseline stiffness prior to odorant stimulation (normalized baseline is indicated by dotted line). For vehicle control, stiffness was measured in response to 0.1% DMSO. As positive controls, cell were contracted with 10 μM histamine and relaxed with 10 μM isoproterenol. Data are presented as mean ± SE ( n = 92 to 403 individual cell measurements). The colors indicated the odor types of 20 volatile chemicals (putrid, nutty, fruity, floral, caramellic, herbaceous, and minty); the concentrations used for each odorant molecule are shown in SI Appendix , Table S1 .
    Flexstation Ii 384, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 97/100, based on 141 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flexstation ii 384/product/Molecular Devices LLC
    Average 97 stars, based on 141 article reviews
    Price from $9.99 to $1999.99
    flexstation ii 384 - by Bioz Stars, 2021-01
    97/100 stars
    		  Buy from Supplier
    flexstation ii  (Molecular Devices LLC)
    97
    Molecular Devices LLC flexstation ii
    Intracellular calcium flux and single-cell contractility evoked by 20 volatile odorants. ( A ) Odorant-evoked [Ca 2+ ] i was measured using FLIPR Calcium 5 assay. For each well of human ASM cells (∼30,000 cells/well), baseline Calcium 5 dye fluorescence was measured for the first 15 s, and after odorant addition (t = 15 s), the fluorescent reading was continuously measured using a FlexStation 3. For vehicle control, [Ca 2+ ] i was measured in response to 0.1% DMSO. As positive control, cells were stimulated with 10 μM histamine. Data are presented as mean ± SE ( n = 3 independent measurements). ( B ) Dynamic changes in the stiffness of human ASM cells were measured in response to each odorant molecule using OMTC. For each individual human ASM cell, baseline stiffness was measured for the first 60 s, and after odorant addition (t = 60 s), stiffness was continuously measured for the next 240 s. For each cell, stiffness was normalized to its baseline stiffness prior to odorant stimulation (normalized baseline is indicated by dotted line). For vehicle control, stiffness was measured in response to 0.1% DMSO. As positive controls, cell were contracted with 10 μM histamine and relaxed with 10 μM isoproterenol. Data are presented as mean ± SE ( n = 92 to 403 individual cell measurements). The colors indicated the odor types of 20 volatile chemicals (putrid, nutty, fruity, floral, caramellic, herbaceous, and minty); the concentrations used for each odorant molecule are shown in SI Appendix , Table S1 .
    Flexstation Ii, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 97/100, based on 698 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flexstation ii/product/Molecular Devices LLC
    Average 97 stars, based on 698 article reviews
    Price from $9.99 to $1999.99
    flexstation ii - by Bioz Stars, 2021-01
    97/100 stars
    		  Buy from Supplier
    flexstation 3 multimode microplate reader  (Molecular Devices LLC)
    90
    Molecular Devices LLC flexstation 3 multimode microplate reader
    Intracellular calcium flux and single-cell contractility evoked by 20 volatile odorants. ( A ) Odorant-evoked [Ca 2+ ] i was measured using FLIPR Calcium 5 assay. For each well of human ASM cells (∼30,000 cells/well), baseline Calcium 5 dye fluorescence was measured for the first 15 s, and after odorant addition (t = 15 s), the fluorescent reading was continuously measured using a FlexStation 3. For vehicle control, [Ca 2+ ] i was measured in response to 0.1% DMSO. As positive control, cells were stimulated with 10 μM histamine. Data are presented as mean ± SE ( n = 3 independent measurements). ( B ) Dynamic changes in the stiffness of human ASM cells were measured in response to each odorant molecule using OMTC. For each individual human ASM cell, baseline stiffness was measured for the first 60 s, and after odorant addition (t = 60 s), stiffness was continuously measured for the next 240 s. For each cell, stiffness was normalized to its baseline stiffness prior to odorant stimulation (normalized baseline is indicated by dotted line). For vehicle control, stiffness was measured in response to 0.1% DMSO. As positive controls, cell were contracted with 10 μM histamine and relaxed with 10 μM isoproterenol. Data are presented as mean ± SE ( n = 92 to 403 individual cell measurements). The colors indicated the odor types of 20 volatile chemicals (putrid, nutty, fruity, floral, caramellic, herbaceous, and minty); the concentrations used for each odorant molecule are shown in SI Appendix , Table S1 .
    Flexstation 3 Multimode Microplate Reader, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 90/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flexstation 3 multimode microplate reader/product/Molecular Devices LLC
    Average 90 stars, based on 67 article reviews
    Price from $9.99 to $1999.99
    flexstation 3 multimode microplate reader - by Bioz Stars, 2021-01
    90/100 stars
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    flexstation iii apparatus  (Molecular Devices LLC)
    93
    Molecular Devices LLC flexstation iii apparatus
    Intracellular calcium flux and single-cell contractility evoked by 20 volatile odorants. ( A ) Odorant-evoked [Ca 2+ ] i was measured using FLIPR Calcium 5 assay. For each well of human ASM cells (∼30,000 cells/well), baseline Calcium 5 dye fluorescence was measured for the first 15 s, and after odorant addition (t = 15 s), the fluorescent reading was continuously measured using a FlexStation 3. For vehicle control, [Ca 2+ ] i was measured in response to 0.1% DMSO. As positive control, cells were stimulated with 10 μM histamine. Data are presented as mean ± SE ( n = 3 independent measurements). ( B ) Dynamic changes in the stiffness of human ASM cells were measured in response to each odorant molecule using OMTC. For each individual human ASM cell, baseline stiffness was measured for the first 60 s, and after odorant addition (t = 60 s), stiffness was continuously measured for the next 240 s. For each cell, stiffness was normalized to its baseline stiffness prior to odorant stimulation (normalized baseline is indicated by dotted line). For vehicle control, stiffness was measured in response to 0.1% DMSO. As positive controls, cell were contracted with 10 μM histamine and relaxed with 10 μM isoproterenol. Data are presented as mean ± SE ( n = 92 to 403 individual cell measurements). The colors indicated the odor types of 20 volatile chemicals (putrid, nutty, fruity, floral, caramellic, herbaceous, and minty); the concentrations used for each odorant molecule are shown in SI Appendix , Table S1 .
    Flexstation Iii Apparatus, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flexstation iii apparatus/product/Molecular Devices LLC
    Average 93 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    flexstation iii apparatus - by Bioz Stars, 2021-01
    93/100 stars
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    flexstation ii scanning fluorometer  (Integrated Fluidics)
    88
    Integrated Fluidics flexstation ii scanning fluorometer
    Intracellular calcium flux and single-cell contractility evoked by 20 volatile odorants. ( A ) Odorant-evoked [Ca 2+ ] i was measured using FLIPR Calcium 5 assay. For each well of human ASM cells (∼30,000 cells/well), baseline Calcium 5 dye fluorescence was measured for the first 15 s, and after odorant addition (t = 15 s), the fluorescent reading was continuously measured using a FlexStation 3. For vehicle control, [Ca 2+ ] i was measured in response to 0.1% DMSO. As positive control, cells were stimulated with 10 μM histamine. Data are presented as mean ± SE ( n = 3 independent measurements). ( B ) Dynamic changes in the stiffness of human ASM cells were measured in response to each odorant molecule using OMTC. For each individual human ASM cell, baseline stiffness was measured for the first 60 s, and after odorant addition (t = 60 s), stiffness was continuously measured for the next 240 s. For each cell, stiffness was normalized to its baseline stiffness prior to odorant stimulation (normalized baseline is indicated by dotted line). For vehicle control, stiffness was measured in response to 0.1% DMSO. As positive controls, cell were contracted with 10 μM histamine and relaxed with 10 μM isoproterenol. Data are presented as mean ± SE ( n = 92 to 403 individual cell measurements). The colors indicated the odor types of 20 volatile chemicals (putrid, nutty, fruity, floral, caramellic, herbaceous, and minty); the concentrations used for each odorant molecule are shown in SI Appendix , Table S1 .
    Flexstation Ii Scanning Fluorometer, supplied by Integrated Fluidics, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flexstation ii scanning fluorometer/product/Integrated Fluidics
    Average 88 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    flexstation ii scanning fluorometer - by Bioz Stars, 2021-01
    88/100 stars
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    flexstation 3 96 well plate reader  (Molecular Devices LLC)
    97
    Molecular Devices LLC flexstation 3 96 well plate reader
    Intracellular calcium flux and single-cell contractility evoked by 20 volatile odorants. ( A ) Odorant-evoked [Ca 2+ ] i was measured using FLIPR Calcium 5 assay. For each well of human ASM cells (∼30,000 cells/well), baseline Calcium 5 dye fluorescence was measured for the first 15 s, and after odorant addition (t = 15 s), the fluorescent reading was continuously measured using a FlexStation 3. For vehicle control, [Ca 2+ ] i was measured in response to 0.1% DMSO. As positive control, cells were stimulated with 10 μM histamine. Data are presented as mean ± SE ( n = 3 independent measurements). ( B ) Dynamic changes in the stiffness of human ASM cells were measured in response to each odorant molecule using OMTC. For each individual human ASM cell, baseline stiffness was measured for the first 60 s, and after odorant addition (t = 60 s), stiffness was continuously measured for the next 240 s. For each cell, stiffness was normalized to its baseline stiffness prior to odorant stimulation (normalized baseline is indicated by dotted line). For vehicle control, stiffness was measured in response to 0.1% DMSO. As positive controls, cell were contracted with 10 μM histamine and relaxed with 10 μM isoproterenol. Data are presented as mean ± SE ( n = 92 to 403 individual cell measurements). The colors indicated the odor types of 20 volatile chemicals (putrid, nutty, fruity, floral, caramellic, herbaceous, and minty); the concentrations used for each odorant molecule are shown in SI Appendix , Table S1 .
    Flexstation 3 96 Well Plate Reader, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 97/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flexstation 3 96 well plate reader/product/Molecular Devices LLC
    Average 97 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    flexstation 3 96 well plate reader - by Bioz Stars, 2021-01
    97/100 stars
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    flexstation 3 multimode plate reader  (Avantor)
    90
    Avantor flexstation 3 multimode plate reader
    Intracellular calcium flux and single-cell contractility evoked by 20 volatile odorants. ( A ) Odorant-evoked [Ca 2+ ] i was measured using FLIPR Calcium 5 assay. For each well of human ASM cells (∼30,000 cells/well), baseline Calcium 5 dye fluorescence was measured for the first 15 s, and after odorant addition (t = 15 s), the fluorescent reading was continuously measured using a FlexStation 3. For vehicle control, [Ca 2+ ] i was measured in response to 0.1% DMSO. As positive control, cells were stimulated with 10 μM histamine. Data are presented as mean ± SE ( n = 3 independent measurements). ( B ) Dynamic changes in the stiffness of human ASM cells were measured in response to each odorant molecule using OMTC. For each individual human ASM cell, baseline stiffness was measured for the first 60 s, and after odorant addition (t = 60 s), stiffness was continuously measured for the next 240 s. For each cell, stiffness was normalized to its baseline stiffness prior to odorant stimulation (normalized baseline is indicated by dotted line). For vehicle control, stiffness was measured in response to 0.1% DMSO. As positive controls, cell were contracted with 10 μM histamine and relaxed with 10 μM isoproterenol. Data are presented as mean ± SE ( n = 92 to 403 individual cell measurements). The colors indicated the odor types of 20 volatile chemicals (putrid, nutty, fruity, floral, caramellic, herbaceous, and minty); the concentrations used for each odorant molecule are shown in SI Appendix , Table S1 .
    Flexstation 3 Multimode Plate Reader, supplied by Avantor, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flexstation 3 multimode plate reader/product/Avantor
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    flexstation 3 multimode plate reader - by Bioz Stars, 2021-01
    90/100 stars
    		  Buy from Supplier
    flexstation calcium assay kit  (Molecular Devices LLC)
    93
    Molecular Devices LLC flexstation calcium assay kit
    Intracellular calcium flux and single-cell contractility evoked by 20 volatile odorants. ( A ) Odorant-evoked [Ca 2+ ] i was measured using FLIPR Calcium 5 assay. For each well of human ASM cells (∼30,000 cells/well), baseline Calcium 5 dye fluorescence was measured for the first 15 s, and after odorant addition (t = 15 s), the fluorescent reading was continuously measured using a FlexStation 3. For vehicle control, [Ca 2+ ] i was measured in response to 0.1% DMSO. As positive control, cells were stimulated with 10 μM histamine. Data are presented as mean ± SE ( n = 3 independent measurements). ( B ) Dynamic changes in the stiffness of human ASM cells were measured in response to each odorant molecule using OMTC. For each individual human ASM cell, baseline stiffness was measured for the first 60 s, and after odorant addition (t = 60 s), stiffness was continuously measured for the next 240 s. For each cell, stiffness was normalized to its baseline stiffness prior to odorant stimulation (normalized baseline is indicated by dotted line). For vehicle control, stiffness was measured in response to 0.1% DMSO. As positive controls, cell were contracted with 10 μM histamine and relaxed with 10 μM isoproterenol. Data are presented as mean ± SE ( n = 92 to 403 individual cell measurements). The colors indicated the odor types of 20 volatile chemicals (putrid, nutty, fruity, floral, caramellic, herbaceous, and minty); the concentrations used for each odorant molecule are shown in SI Appendix , Table S1 .
    Flexstation Calcium Assay Kit, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 93/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flexstation calcium assay kit/product/Molecular Devices LLC
    Average 93 stars, based on 51 article reviews
    Price from $9.99 to $1999.99
    flexstation calcium assay kit - by Bioz Stars, 2021-01
    93/100 stars
    		  Buy from Supplier
    flexstation ii plate reader  (Molecular Devices LLC)
    99
    Molecular Devices LLC flexstation ii plate reader
    Intracellular calcium flux and single-cell contractility evoked by 20 volatile odorants. ( A ) Odorant-evoked [Ca 2+ ] i was measured using FLIPR Calcium 5 assay. For each well of human ASM cells (∼30,000 cells/well), baseline Calcium 5 dye fluorescence was measured for the first 15 s, and after odorant addition (t = 15 s), the fluorescent reading was continuously measured using a FlexStation 3. For vehicle control, [Ca 2+ ] i was measured in response to 0.1% DMSO. As positive control, cells were stimulated with 10 μM histamine. Data are presented as mean ± SE ( n = 3 independent measurements). ( B ) Dynamic changes in the stiffness of human ASM cells were measured in response to each odorant molecule using OMTC. For each individual human ASM cell, baseline stiffness was measured for the first 60 s, and after odorant addition (t = 60 s), stiffness was continuously measured for the next 240 s. For each cell, stiffness was normalized to its baseline stiffness prior to odorant stimulation (normalized baseline is indicated by dotted line). For vehicle control, stiffness was measured in response to 0.1% DMSO. As positive controls, cell were contracted with 10 μM histamine and relaxed with 10 μM isoproterenol. Data are presented as mean ± SE ( n = 92 to 403 individual cell measurements). The colors indicated the odor types of 20 volatile chemicals (putrid, nutty, fruity, floral, caramellic, herbaceous, and minty); the concentrations used for each odorant molecule are shown in SI Appendix , Table S1 .
    Flexstation Ii Plate Reader, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 99/100, based on 209 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flexstation ii plate reader/product/Molecular Devices LLC
    Average 99 stars, based on 209 article reviews
    Price from $9.99 to $1999.99
    flexstation ii plate reader - by Bioz Stars, 2021-01
    99/100 stars
    		  Buy from Supplier
    flexstation multilabel plate reader  (Molecular Devices LLC)
    90
    Molecular Devices LLC flexstation multilabel plate reader
    Intracellular calcium flux and single-cell contractility evoked by 20 volatile odorants. ( A ) Odorant-evoked [Ca 2+ ] i was measured using FLIPR Calcium 5 assay. For each well of human ASM cells (∼30,000 cells/well), baseline Calcium 5 dye fluorescence was measured for the first 15 s, and after odorant addition (t = 15 s), the fluorescent reading was continuously measured using a FlexStation 3. For vehicle control, [Ca 2+ ] i was measured in response to 0.1% DMSO. As positive control, cells were stimulated with 10 μM histamine. Data are presented as mean ± SE ( n = 3 independent measurements). ( B ) Dynamic changes in the stiffness of human ASM cells were measured in response to each odorant molecule using OMTC. For each individual human ASM cell, baseline stiffness was measured for the first 60 s, and after odorant addition (t = 60 s), stiffness was continuously measured for the next 240 s. For each cell, stiffness was normalized to its baseline stiffness prior to odorant stimulation (normalized baseline is indicated by dotted line). For vehicle control, stiffness was measured in response to 0.1% DMSO. As positive controls, cell were contracted with 10 μM histamine and relaxed with 10 μM isoproterenol. Data are presented as mean ± SE ( n = 92 to 403 individual cell measurements). The colors indicated the odor types of 20 volatile chemicals (putrid, nutty, fruity, floral, caramellic, herbaceous, and minty); the concentrations used for each odorant molecule are shown in SI Appendix , Table S1 .
    Flexstation Multilabel Plate Reader, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flexstation multilabel plate reader/product/Molecular Devices LLC
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    flexstation multilabel plate reader - by Bioz Stars, 2021-01
    90/100 stars
    		  Buy from Supplier
    molecular devices flexstation 3  (Molecular Devices LLC)
    97
    Molecular Devices LLC molecular devices flexstation 3
    Intracellular calcium flux and single-cell contractility evoked by 20 volatile odorants. ( A ) Odorant-evoked [Ca 2+ ] i was measured using FLIPR Calcium 5 assay. For each well of human ASM cells (∼30,000 cells/well), baseline Calcium 5 dye fluorescence was measured for the first 15 s, and after odorant addition (t = 15 s), the fluorescent reading was continuously measured using a FlexStation 3. For vehicle control, [Ca 2+ ] i was measured in response to 0.1% DMSO. As positive control, cells were stimulated with 10 μM histamine. Data are presented as mean ± SE ( n = 3 independent measurements). ( B ) Dynamic changes in the stiffness of human ASM cells were measured in response to each odorant molecule using OMTC. For each individual human ASM cell, baseline stiffness was measured for the first 60 s, and after odorant addition (t = 60 s), stiffness was continuously measured for the next 240 s. For each cell, stiffness was normalized to its baseline stiffness prior to odorant stimulation (normalized baseline is indicated by dotted line). For vehicle control, stiffness was measured in response to 0.1% DMSO. As positive controls, cell were contracted with 10 μM histamine and relaxed with 10 μM isoproterenol. Data are presented as mean ± SE ( n = 92 to 403 individual cell measurements). The colors indicated the odor types of 20 volatile chemicals (putrid, nutty, fruity, floral, caramellic, herbaceous, and minty); the concentrations used for each odorant molecule are shown in SI Appendix , Table S1 .
    Molecular Devices Flexstation 3, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 97/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/molecular devices flexstation 3/product/Molecular Devices LLC
    Average 97 stars, based on 42 article reviews
    Price from $9.99 to $1999.99
    molecular devices flexstation 3 - by Bioz Stars, 2021-01
    97/100 stars
    		  Buy from Supplier
    flexstation 3 plate reader  (BioTek Instruments)
    97
    BioTek Instruments flexstation 3 plate reader
    Intracellular calcium flux and single-cell contractility evoked by 20 volatile odorants. ( A ) Odorant-evoked [Ca 2+ ] i was measured using FLIPR Calcium 5 assay. For each well of human ASM cells (∼30,000 cells/well), baseline Calcium 5 dye fluorescence was measured for the first 15 s, and after odorant addition (t = 15 s), the fluorescent reading was continuously measured using a FlexStation 3. For vehicle control, [Ca 2+ ] i was measured in response to 0.1% DMSO. As positive control, cells were stimulated with 10 μM histamine. Data are presented as mean ± SE ( n = 3 independent measurements). ( B ) Dynamic changes in the stiffness of human ASM cells were measured in response to each odorant molecule using OMTC. For each individual human ASM cell, baseline stiffness was measured for the first 60 s, and after odorant addition (t = 60 s), stiffness was continuously measured for the next 240 s. For each cell, stiffness was normalized to its baseline stiffness prior to odorant stimulation (normalized baseline is indicated by dotted line). For vehicle control, stiffness was measured in response to 0.1% DMSO. As positive controls, cell were contracted with 10 μM histamine and relaxed with 10 μM isoproterenol. Data are presented as mean ± SE ( n = 92 to 403 individual cell measurements). The colors indicated the odor types of 20 volatile chemicals (putrid, nutty, fruity, floral, caramellic, herbaceous, and minty); the concentrations used for each odorant molecule are shown in SI Appendix , Table S1 .
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    Intracellular calcium flux and single-cell contractility evoked by 20 volatile odorants. ( A ) Odorant-evoked [Ca 2+ ] i was measured using FLIPR Calcium 5 assay. For each well of human ASM cells (∼30,000 cells/well), baseline Calcium 5 dye fluorescence was measured for the first 15 s, and after odorant addition (t = 15 s), the fluorescent reading was continuously measured using a FlexStation 3. For vehicle control, [Ca 2+ ] i was measured in response to 0.1% DMSO. As positive control, cells were stimulated with 10 μM histamine. Data are presented as mean ± SE ( n = 3 independent measurements). ( B ) Dynamic changes in the stiffness of human ASM cells were measured in response to each odorant molecule using OMTC. For each individual human ASM cell, baseline stiffness was measured for the first 60 s, and after odorant addition (t = 60 s), stiffness was continuously measured for the next 240 s. For each cell, stiffness was normalized to its baseline stiffness prior to odorant stimulation (normalized baseline is indicated by dotted line). For vehicle control, stiffness was measured in response to 0.1% DMSO. As positive controls, cell were contracted with 10 μM histamine and relaxed with 10 μM isoproterenol. Data are presented as mean ± SE ( n = 92 to 403 individual cell measurements). The colors indicated the odor types of 20 volatile chemicals (putrid, nutty, fruity, floral, caramellic, herbaceous, and minty); the concentrations used for each odorant molecule are shown in SI Appendix , Table S1 .

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: The odorant receptor OR2W3 on airway smooth muscle evokes bronchodilation via a cooperative chemosensory tradeoff between TMEM16A and CFTR

    doi: 10.1073/pnas.2003111117

    Figure Lengend Snippet: Intracellular calcium flux and single-cell contractility evoked by 20 volatile odorants. ( A ) Odorant-evoked [Ca 2+ ] i was measured using FLIPR Calcium 5 assay. For each well of human ASM cells (∼30,000 cells/well), baseline Calcium 5 dye fluorescence was measured for the first 15 s, and after odorant addition (t = 15 s), the fluorescent reading was continuously measured using a FlexStation 3. For vehicle control, [Ca 2+ ] i was measured in response to 0.1% DMSO. As positive control, cells were stimulated with 10 μM histamine. Data are presented as mean ± SE ( n = 3 independent measurements). ( B ) Dynamic changes in the stiffness of human ASM cells were measured in response to each odorant molecule using OMTC. For each individual human ASM cell, baseline stiffness was measured for the first 60 s, and after odorant addition (t = 60 s), stiffness was continuously measured for the next 240 s. For each cell, stiffness was normalized to its baseline stiffness prior to odorant stimulation (normalized baseline is indicated by dotted line). For vehicle control, stiffness was measured in response to 0.1% DMSO. As positive controls, cell were contracted with 10 μM histamine and relaxed with 10 μM isoproterenol. Data are presented as mean ± SE ( n = 92 to 403 individual cell measurements). The colors indicated the odor types of 20 volatile chemicals (putrid, nutty, fruity, floral, caramellic, herbaceous, and minty); the concentrations used for each odorant molecule are shown in SI Appendix , Table S1 .

    Article Snippet: Fluorescence signals were measured using a FlexStation 3 (Molecular Devices), with baseline fluorescence (excitation 530 nm, emission 565 nm, cutoff 550 nm) measurements taken for 16 s before addition of agonists.

    Techniques: Fluorescence, Positive Control