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    Vector Laboratories fluoresceinvicia villosa lectin vva
    ( A–B ) in situ hybridization for dumpy mRNA in the lobed species D. melanogaster ( A ) and the non-lobed species D. biarmipes ( B ). Pink box outlines location of zoomed in images presented in A1 and B1. Relevant expression highlighted with arrow (purple/white) for strong expression, asterisk for weak expression, and arrowhead for clasper-specific expression. Expression observed in D. melanogaster at 44 hr APF is not present in all samples (see ). ( C–D ) aECM is labeled with Vicia <t>villosa</t> <t>lectin</t> <t>(VVA;</t> green) and apical membrane labeled with E-cadherin (Ecad; magenta) at 44 hr APF in D. melanogaster ( C ) and D. biarmipes ( D ). Location of respective cross sections indicated in yellow for lateral plate ( C2–D2 ) and blue for posterior lobe in D. melanogaster ( C1 ) and corresponding position in D. biarmipes ( D1 ). All cross-sections are oriented with apical side at the top and basal side at the bottom. White arrows highlight the crevice localization between the lateral plate and clasper, which the aECM fills in D. melanogaster ( C1 ), but only a weakly stained strand-like structure of aECM appears in D. biarmipes ( D1 ). Tendrils of aECM can also be observed connecting to the lateral plate in both species (red arrowheads). Relevant structures labeled: Posterior lobe (PL), lateral plate (LP), clasper (C), sheath (S), and phallus (P). Scale bar, 20 μm. n = at least five per experiment.
    Fluoresceinvicia Villosa Lectin Vva, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluoresceinvicia villosa lectin vva/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
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    fluoresceinvicia villosa lectin vva - by Bioz Stars, 2024-06
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    Image Search Results


    ( A–B ) in situ hybridization for dumpy mRNA in the lobed species D. melanogaster ( A ) and the non-lobed species D. biarmipes ( B ). Pink box outlines location of zoomed in images presented in A1 and B1. Relevant expression highlighted with arrow (purple/white) for strong expression, asterisk for weak expression, and arrowhead for clasper-specific expression. Expression observed in D. melanogaster at 44 hr APF is not present in all samples (see ). ( C–D ) aECM is labeled with Vicia villosa lectin (VVA; green) and apical membrane labeled with E-cadherin (Ecad; magenta) at 44 hr APF in D. melanogaster ( C ) and D. biarmipes ( D ). Location of respective cross sections indicated in yellow for lateral plate ( C2–D2 ) and blue for posterior lobe in D. melanogaster ( C1 ) and corresponding position in D. biarmipes ( D1 ). All cross-sections are oriented with apical side at the top and basal side at the bottom. White arrows highlight the crevice localization between the lateral plate and clasper, which the aECM fills in D. melanogaster ( C1 ), but only a weakly stained strand-like structure of aECM appears in D. biarmipes ( D1 ). Tendrils of aECM can also be observed connecting to the lateral plate in both species (red arrowheads). Relevant structures labeled: Posterior lobe (PL), lateral plate (LP), clasper (C), sheath (S), and phallus (P). Scale bar, 20 μm. n = at least five per experiment.

    Journal: eLife

    Article Title: Evolutionary expansion of apical extracellular matrix is required for the elongation of cells in a novel structure

    doi: 10.7554/eLife.55965

    Figure Lengend Snippet: ( A–B ) in situ hybridization for dumpy mRNA in the lobed species D. melanogaster ( A ) and the non-lobed species D. biarmipes ( B ). Pink box outlines location of zoomed in images presented in A1 and B1. Relevant expression highlighted with arrow (purple/white) for strong expression, asterisk for weak expression, and arrowhead for clasper-specific expression. Expression observed in D. melanogaster at 44 hr APF is not present in all samples (see ). ( C–D ) aECM is labeled with Vicia villosa lectin (VVA; green) and apical membrane labeled with E-cadherin (Ecad; magenta) at 44 hr APF in D. melanogaster ( C ) and D. biarmipes ( D ). Location of respective cross sections indicated in yellow for lateral plate ( C2–D2 ) and blue for posterior lobe in D. melanogaster ( C1 ) and corresponding position in D. biarmipes ( D1 ). All cross-sections are oriented with apical side at the top and basal side at the bottom. White arrows highlight the crevice localization between the lateral plate and clasper, which the aECM fills in D. melanogaster ( C1 ), but only a weakly stained strand-like structure of aECM appears in D. biarmipes ( D1 ). Tendrils of aECM can also be observed connecting to the lateral plate in both species (red arrowheads). Relevant structures labeled: Posterior lobe (PL), lateral plate (LP), clasper (C), sheath (S), and phallus (P). Scale bar, 20 μm. n = at least five per experiment.

    Article Snippet: Lectin , fluoresceinVicia Villosa Lectin (VVA) , Vector Laboratories , Vector Laboratories Cat# FL-1231, RRID: AB_2336856 , IHC (1:200).

    Techniques: In Situ Hybridization, Expressing, Labeling, Staining

    Journal: eLife

    Article Title: Evolutionary expansion of apical extracellular matrix is required for the elongation of cells in a novel structure

    doi: 10.7554/eLife.55965

    Figure Lengend Snippet:

    Article Snippet: Lectin , fluoresceinVicia Villosa Lectin (VVA) , Vector Laboratories , Vector Laboratories Cat# FL-1231, RRID: AB_2336856 , IHC (1:200).

    Techniques: Plasmid Preparation, Construct, Recombinant, Sequencing, Software