Article Title: DNA damage repair alterations modulate M2 polarization of microglia to remodel the tumor microenvironment via the p53-mediated MDK expression in glioma
Figure Lengend Snippet: Photomicrographs of M2 microglia following GCM stimulation of glioma cells in vitro and in vivo. (a) The human microglia HMC3 cells were treated with GCM from LN229, LN229R, HG7 and HG7R cells. (b) HMC3 were treated with GCM from LN229-Scr, LN229-OE, HG7-Scr and HG7-OE cells. (c) BV-2 cells were treated with GCM from GL261, GL261R, GL261-Scr and GL261-OE cells. CD68, CD163, CD204 and CD206 were stained in red, while Arg1, Mrc1, Fizz1 and Cd163 were stained in green. Nuclei were stained with DAPI (blue). (d) IHC assays were performed to detect Arg1, Mrc1, Fizz1 and Cd163 in xenograft gliomas formed by GL261-Scr and GL261-OE cells. All images were taken microscopically (20× or 40×). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Article Snippet: Briefly, the slices were incubated with primary antibody rabbit anti-C5 (1:100, ABclonal, A8104), rabbit anti-IL6 (1:200, ABclonal, A0286), mouse anti-TNFSF4 (1:200, Abcam, ab89896), rabbit anti-TNFSF4 (1:200, ThermoFisher, #MA5–17910), rabbit anti-SAA1 (1:200, Abcam, ab207445), rabbit anti-SAA1 (1:200, Abcam, ab199030), rabbit anti-MDK (1:200, Abcam, ab170820), mouse anti-VEGFA (1:200, Abcam, ab1316), rabbit anti-CD68 (1:200, Abcam, ab125212), rabbit anti-CD163 (1:200, Proteintech, 16,646–1-AP), rabbit anti-CD204 (1:200, Abcam, ab123946), rabbit anti-CD206 (1:200, Abcam, ab64693), mouse anti-Arg1 (1:200, Abcam, ab239731), rabbit anti-Fizz1 (1:200, Abcam, ab39626) and rabbit anti-Mrc1 (1:200, Abcam, ab64693) for 12 h at 4 °C.
Techniques: In Vitro, In Vivo, Staining, Immunohistochemistry