fitc labeled monoclonal anti-cd40l antibody Search Results


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  • 80
    Pharmingen pe labeled anti cd40l mab mr 1
    Pe Labeled Anti Cd40l Mab Mr 1, supplied by Pharmingen, used in various techniques. Bioz Stars score: 80/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pe labeled anti cd40l mab mr 1/product/Pharmingen
    Average 80 stars, based on 3 article reviews
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    pe labeled anti cd40l mab mr 1 - by Bioz Stars, 2021-01
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    80
    Pharmingen pe labeled mab against murine cd40l
    Inhibition of NSI HIV-1 replication in CD4 + T cells by <t>CD40L-stimulated</t> macrophage supernatants. CD4 + T cells (depleted of CD8 + T cells) were stimulated by PHA and IL-2, infected with the NSI isolate, (HIV-1 SF162 ), and cultured for 14 days in either R10 medium or a 1:20 dilution of MDM supernatants. Virus production was measured by ELISA for HIV-1 p24 antigen. To demonstrate that the β-chemokines in the CD40L-stimulated MDM supernatant accounted for the HIV-1 suppressive activity, the supernatant was pretreated with a mixture of neutralizing antibodies against MIP-1α, MIP-1β, and RANTES, which abrogated its effectiveness.
    Pe Labeled Mab Against Murine Cd40l, supplied by Pharmingen, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pe labeled mab against murine cd40l/product/Pharmingen
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pe labeled mab against murine cd40l - by Bioz Stars, 2021-01
    80/100 stars
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    92
    Abcam fitc labeled monoclonal anti cd40l antibody
    Inhibition of NSI HIV-1 replication in CD4 + T cells by <t>CD40L-stimulated</t> macrophage supernatants. CD4 + T cells (depleted of CD8 + T cells) were stimulated by PHA and IL-2, infected with the NSI isolate, (HIV-1 SF162 ), and cultured for 14 days in either R10 medium or a 1:20 dilution of MDM supernatants. Virus production was measured by ELISA for HIV-1 p24 antigen. To demonstrate that the β-chemokines in the CD40L-stimulated MDM supernatant accounted for the HIV-1 suppressive activity, the supernatant was pretreated with a mixture of neutralizing antibodies against MIP-1α, MIP-1β, and RANTES, which abrogated its effectiveness.
    Fitc Labeled Monoclonal Anti Cd40l Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc labeled monoclonal anti cd40l antibody/product/Abcam
    Average 92 stars, based on 70 article reviews
    Price from $9.99 to $1999.99
    fitc labeled monoclonal anti cd40l antibody - by Bioz Stars, 2021-01
    92/100 stars
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    99
    Becton Dickinson fitc labelled anti cd40l mab
    Inhibition of NSI HIV-1 replication in CD4 + T cells by <t>CD40L-stimulated</t> macrophage supernatants. CD4 + T cells (depleted of CD8 + T cells) were stimulated by PHA and IL-2, infected with the NSI isolate, (HIV-1 SF162 ), and cultured for 14 days in either R10 medium or a 1:20 dilution of MDM supernatants. Virus production was measured by ELISA for HIV-1 p24 antigen. To demonstrate that the β-chemokines in the CD40L-stimulated MDM supernatant accounted for the HIV-1 suppressive activity, the supernatant was pretreated with a mixture of neutralizing antibodies against MIP-1α, MIP-1β, and RANTES, which abrogated its effectiveness.
    Fitc Labelled Anti Cd40l Mab, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc labelled anti cd40l mab/product/Becton Dickinson
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    fitc labelled anti cd40l mab - by Bioz Stars, 2021-01
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    80
    Ancell corporation phycoerythrin pe labeled mab against human cd40l clone 24 31
    Inhibition of NSI HIV-1 replication in CD4 + T cells by <t>CD40L-stimulated</t> macrophage supernatants. CD4 + T cells (depleted of CD8 + T cells) were stimulated by PHA and IL-2, infected with the NSI isolate, (HIV-1 SF162 ), and cultured for 14 days in either R10 medium or a 1:20 dilution of MDM supernatants. Virus production was measured by ELISA for HIV-1 p24 antigen. To demonstrate that the β-chemokines in the CD40L-stimulated MDM supernatant accounted for the HIV-1 suppressive activity, the supernatant was pretreated with a mixture of neutralizing antibodies against MIP-1α, MIP-1β, and RANTES, which abrogated its effectiveness.
    Phycoerythrin Pe Labeled Mab Against Human Cd40l Clone 24 31, supplied by Ancell corporation, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phycoerythrin pe labeled mab against human cd40l clone 24 31/product/Ancell corporation
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phycoerythrin pe labeled mab against human cd40l clone 24 31 - by Bioz Stars, 2021-01
    80/100 stars
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    Inhibition of NSI HIV-1 replication in CD4 + T cells by CD40L-stimulated macrophage supernatants. CD4 + T cells (depleted of CD8 + T cells) were stimulated by PHA and IL-2, infected with the NSI isolate, (HIV-1 SF162 ), and cultured for 14 days in either R10 medium or a 1:20 dilution of MDM supernatants. Virus production was measured by ELISA for HIV-1 p24 antigen. To demonstrate that the β-chemokines in the CD40L-stimulated MDM supernatant accounted for the HIV-1 suppressive activity, the supernatant was pretreated with a mixture of neutralizing antibodies against MIP-1α, MIP-1β, and RANTES, which abrogated its effectiveness.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: CD40 ligand (CD154) stimulation of macrophages to produce HIV-1-suppressive ?-chemokines

    doi:

    Figure Lengend Snippet: Inhibition of NSI HIV-1 replication in CD4 + T cells by CD40L-stimulated macrophage supernatants. CD4 + T cells (depleted of CD8 + T cells) were stimulated by PHA and IL-2, infected with the NSI isolate, (HIV-1 SF162 ), and cultured for 14 days in either R10 medium or a 1:20 dilution of MDM supernatants. Virus production was measured by ELISA for HIV-1 p24 antigen. To demonstrate that the β-chemokines in the CD40L-stimulated MDM supernatant accounted for the HIV-1 suppressive activity, the supernatant was pretreated with a mixture of neutralizing antibodies against MIP-1α, MIP-1β, and RANTES, which abrogated its effectiveness.

    Article Snippet: PE-labeled mAb against murine CD40L (clone MR1) was from PharMingen.

    Techniques: Inhibition, Infection, Cell Culture, Enzyme-linked Immunosorbent Assay, Activity Assay

    CD40L induction of β-chemokine production by macrophages. ( A ) Flow cytometry measurement of CD40L surface expression. Isotype control staining of CD40L-293 cells with PE-conjugated anti-CD8 control mAb (thin solid line). Expression of human CD40L on control 293 cells (thin broken line) and CD40L-293 cells (thick solid line) assessed by using PE-conjugated anti-CD40L mAb 24–31. ( B ) β-chemokine production by macrophages. MDM were cultured in medium alone, with added IFN-γ or GM-CSF, or with added control 293 cells or CD40L-293 cells. LPS was used as a positive control. The mean chemokine concentrations (picogram per milliliter) of the supernatants from quadruplicate wells 24 hr later are shown (±SD). □, MIP-1α; ▨, MIP-1β; ▪, RANTES. ( C ) Abrogation of CD40L stimulation by anti-CD40 or anti-CD40L mAbs. In an experiment similar to B , neutralizing mAbs were added at the initiation of the CD40L-293 cell-MDM cocultures. Anti-CD40 mAb blocked > 99% and anti-CD40L mAb blocked 94% of the β-chemokine release. ( D ) Stimulation of macrophages by CD40L-bearing plasma membranes. Acellular preparations of membranes from control 293 cells, 293 cells expressing a nonfunctional mutant of human CD40L (T147N), human CD40L-293 cells (CD40L), and murine CD40L-293 cells (mCD40L) were added to MDM in an experiment similar to B .

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: CD40 ligand (CD154) stimulation of macrophages to produce HIV-1-suppressive ?-chemokines

    doi:

    Figure Lengend Snippet: CD40L induction of β-chemokine production by macrophages. ( A ) Flow cytometry measurement of CD40L surface expression. Isotype control staining of CD40L-293 cells with PE-conjugated anti-CD8 control mAb (thin solid line). Expression of human CD40L on control 293 cells (thin broken line) and CD40L-293 cells (thick solid line) assessed by using PE-conjugated anti-CD40L mAb 24–31. ( B ) β-chemokine production by macrophages. MDM were cultured in medium alone, with added IFN-γ or GM-CSF, or with added control 293 cells or CD40L-293 cells. LPS was used as a positive control. The mean chemokine concentrations (picogram per milliliter) of the supernatants from quadruplicate wells 24 hr later are shown (±SD). □, MIP-1α; ▨, MIP-1β; ▪, RANTES. ( C ) Abrogation of CD40L stimulation by anti-CD40 or anti-CD40L mAbs. In an experiment similar to B , neutralizing mAbs were added at the initiation of the CD40L-293 cell-MDM cocultures. Anti-CD40 mAb blocked > 99% and anti-CD40L mAb blocked 94% of the β-chemokine release. ( D ) Stimulation of macrophages by CD40L-bearing plasma membranes. Acellular preparations of membranes from control 293 cells, 293 cells expressing a nonfunctional mutant of human CD40L (T147N), human CD40L-293 cells (CD40L), and murine CD40L-293 cells (mCD40L) were added to MDM in an experiment similar to B .

    Article Snippet: PE-labeled mAb against murine CD40L (clone MR1) was from PharMingen.

    Techniques: Flow Cytometry, Cytometry, Expressing, Staining, Cell Culture, Positive Control, Mutagenesis