fitc conjugated secondary antibody Search Results


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  • 99
    Thermo Fisher alexafluor fluorescein isothiocyanate fitc conjugated secondary antibody
    Alexafluor Fluorescein Isothiocyanate Fitc Conjugated Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexafluor fluorescein isothiocyanate fitc conjugated secondary antibody/product/Thermo Fisher
    Average 99 stars, based on 7 article reviews
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    99
    Abcam fluorescein isothiocyanate fitc conjugated secondary antibodies
    PAT-SM6 specifically binds to MM cell lines and primary human MM samples. A, INA-6, NCI H929, MM1.S, OPM-2 and U266 MM cell lines or CD138-purified patient MM cells were stained with PAT-SM6 (5 µg/mL, open histogram) or isotype control (gray histogram) followed by fluorescein <t>isothiocyanate</t> <t>(FITC)-conjugated</t> secondary antibodies and assessed by flow cytometry. Both, cell lines as well as primary MM cells displayed specific PAT-SM6 binding. B, Immunohistochemistry with PAT-SM6, CD138-positive control antibody or isotype on bone marrow biopsies of MM patients. PAT-SM6 specifically stained MM cells with a homogenous binding pattern. In samples without MM infiltration no specific binding was detected. Images were captured using a Leica DM BL microscope, the Leica ICC HD digital camera and the Leica LAS EZ V2.1.0 software.
    Fluorescein Isothiocyanate Fitc Conjugated Secondary Antibodies, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 244 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Abcam antibodies conjugated to fitc
    PAT-SM6 specifically binds to MM cell lines and primary human MM samples. A, INA-6, NCI H929, MM1.S, OPM-2 and U266 MM cell lines or CD138-purified patient MM cells were stained with PAT-SM6 (5 µg/mL, open histogram) or isotype control (gray histogram) followed by fluorescein <t>isothiocyanate</t> <t>(FITC)-conjugated</t> secondary antibodies and assessed by flow cytometry. Both, cell lines as well as primary MM cells displayed specific PAT-SM6 binding. B, Immunohistochemistry with PAT-SM6, CD138-positive control antibody or isotype on bone marrow biopsies of MM patients. PAT-SM6 specifically stained MM cells with a homogenous binding pattern. In samples without MM infiltration no specific binding was detected. Images were captured using a Leica DM BL microscope, the Leica ICC HD digital camera and the Leica LAS EZ V2.1.0 software.
    Antibodies Conjugated To Fitc, supplied by Abcam, used in various techniques. Bioz Stars score: 90/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher fitc conjugated
    PAT-SM6 specifically binds to MM cell lines and primary human MM samples. A, INA-6, NCI H929, MM1.S, OPM-2 and U266 MM cell lines or CD138-purified patient MM cells were stained with PAT-SM6 (5 µg/mL, open histogram) or isotype control (gray histogram) followed by fluorescein <t>isothiocyanate</t> <t>(FITC)-conjugated</t> secondary antibodies and assessed by flow cytometry. Both, cell lines as well as primary MM cells displayed specific PAT-SM6 binding. B, Immunohistochemistry with PAT-SM6, CD138-positive control antibody or isotype on bone marrow biopsies of MM patients. PAT-SM6 specifically stained MM cells with a homogenous binding pattern. In samples without MM infiltration no specific binding was detected. Images were captured using a Leica DM BL microscope, the Leica ICC HD digital camera and the Leica LAS EZ V2.1.0 software.
    Fitc Conjugated, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 584 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher fluorescein 5 isothiocyanate fitc conjugated secondary antibodies
    PAT-SM6 specifically binds to MM cell lines and primary human MM samples. A, INA-6, NCI H929, MM1.S, OPM-2 and U266 MM cell lines or CD138-purified patient MM cells were stained with PAT-SM6 (5 µg/mL, open histogram) or isotype control (gray histogram) followed by fluorescein <t>isothiocyanate</t> <t>(FITC)-conjugated</t> secondary antibodies and assessed by flow cytometry. Both, cell lines as well as primary MM cells displayed specific PAT-SM6 binding. B, Immunohistochemistry with PAT-SM6, CD138-positive control antibody or isotype on bone marrow biopsies of MM patients. PAT-SM6 specifically stained MM cells with a homogenous binding pattern. In samples without MM infiltration no specific binding was detected. Images were captured using a Leica DM BL microscope, the Leica ICC HD digital camera and the Leica LAS EZ V2.1.0 software.
    Fluorescein 5 Isothiocyanate Fitc Conjugated Secondary Antibodies, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 7 article reviews
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    99
    Bio-Rad fitc conjugated secondary antibody
    PAT-SM6 specifically binds to MM cell lines and primary human MM samples. A, INA-6, NCI H929, MM1.S, OPM-2 and U266 MM cell lines or CD138-purified patient MM cells were stained with PAT-SM6 (5 µg/mL, open histogram) or isotype control (gray histogram) followed by fluorescein <t>isothiocyanate</t> <t>(FITC)-conjugated</t> secondary antibodies and assessed by flow cytometry. Both, cell lines as well as primary MM cells displayed specific PAT-SM6 binding. B, Immunohistochemistry with PAT-SM6, CD138-positive control antibody or isotype on bone marrow biopsies of MM patients. PAT-SM6 specifically stained MM cells with a homogenous binding pattern. In samples without MM infiltration no specific binding was detected. Images were captured using a Leica DM BL microscope, the Leica ICC HD digital camera and the Leica LAS EZ V2.1.0 software.
    Fitc Conjugated Secondary Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc conjugated secondary antibody/product/Bio-Rad
    Average 99 stars, based on 17 article reviews
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    92
    SouthernBiotech fitc conjugated secondary antibody
    PAT-SM6 specifically binds to MM cell lines and primary human MM samples. A, INA-6, NCI H929, MM1.S, OPM-2 and U266 MM cell lines or CD138-purified patient MM cells were stained with PAT-SM6 (5 µg/mL, open histogram) or isotype control (gray histogram) followed by fluorescein <t>isothiocyanate</t> <t>(FITC)-conjugated</t> secondary antibodies and assessed by flow cytometry. Both, cell lines as well as primary MM cells displayed specific PAT-SM6 binding. B, Immunohistochemistry with PAT-SM6, CD138-positive control antibody or isotype on bone marrow biopsies of MM patients. PAT-SM6 specifically stained MM cells with a homogenous binding pattern. In samples without MM infiltration no specific binding was detected. Images were captured using a Leica DM BL microscope, the Leica ICC HD digital camera and the Leica LAS EZ V2.1.0 software.
    Fitc Conjugated Secondary Antibody, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 92/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies fitc conjugated secondary antibody
    Selection of ASLV-B infected DF-1 cells that do not undergo low pH-mediated cell-cell fusion. (A) Selection scheme used to identify subgroup B ASLV-infected cells that are resistant to low pH-mediated syncytia formation. (B) The numbers of hygromycin B-resistant virus-infected colonies that resulted from cell-cell fusion experiments preformed with <t>293:TVB</t> S3 Δ DD cells and either starting population of RCASH-B infected cells or R6 (pH 5.6) cells, are shown. This experiment was performed in triplicate and the average mean values obtained are shown along with the standard deviation of the data. (C) Flow cytometric analysis of EnvB expression. Uninfected cells (green histogram), the starting population of RCASH-B infected DF-1 cells (red histogram, upper panel) and the R6 (pH 5.6) cells (blue histogram, lower panel) were incubated with TVB S3 -rIgG and a <t>FITC-conjugated</t> anti-rabbit antibody and analyzed by flow cytometry as described previously [5] , [27] .
    Fitc Conjugated Secondary Antibody, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 279 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology fitc conjugated secondary antibodies
    Effects of <t>Pin1</t> on proliferation, cell cycle progression, apoptosis and migration in vascular smooth muscle cells (VSMCs). VSMCs were cultured and infected as described in Figure 3 . Cell proliferation was assessed by both the MTT assay ( A ) and [ 3 H]-thymidine incorporation analyses ( B ). Cell cycle analysis was quantified by PI staining followed by flow cytometry analyses. M1, M2, M3 and M4 indicate sub G1, G0/G1, S and G2/M phases respectively ( C ). Bar graphs represent the mean ± SEM of three independent experiments ( D ). Cell apoptosis was detected by annexin <t>V-FITC/PI</t> staining ( E ) followed by flow cytometry analysis and TUNEL staining ( G ). The proportion of live cells (annexin V−/PI−), early apoptotic cells (annexin V+/PI−), late apoptotic/necrotic cells (annexin V+/PI+) and dead cells (annexin V−/PI+) was calculated for comparison ( F ). The TUNEL-positive cells were stained green, VSMCs were stained for SMαA and shown in red. Nuclei were stained blue by DAPI. The percentages of apoptotic nuclei were calculated by determining the number of DAPI-stained nuclei that were also positive for TUNEL staining. Approximately, 100 nuclei cells were counted in randomly chosen fields per region ( H ). VSMC migration was determined by a standard wound healing assay ( I ). Bar graphs represent the mean ± SEM of three independent experiments ( J ). * P
    Fitc Conjugated Secondary Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 256 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    The Jackson Laboratory fitc conjugated secondary antibodies
    Effects of <t>Pin1</t> on proliferation, cell cycle progression, apoptosis and migration in vascular smooth muscle cells (VSMCs). VSMCs were cultured and infected as described in Figure 3 . Cell proliferation was assessed by both the MTT assay ( A ) and [ 3 H]-thymidine incorporation analyses ( B ). Cell cycle analysis was quantified by PI staining followed by flow cytometry analyses. M1, M2, M3 and M4 indicate sub G1, G0/G1, S and G2/M phases respectively ( C ). Bar graphs represent the mean ± SEM of three independent experiments ( D ). Cell apoptosis was detected by annexin <t>V-FITC/PI</t> staining ( E ) followed by flow cytometry analysis and TUNEL staining ( G ). The proportion of live cells (annexin V−/PI−), early apoptotic cells (annexin V+/PI−), late apoptotic/necrotic cells (annexin V+/PI+) and dead cells (annexin V−/PI+) was calculated for comparison ( F ). The TUNEL-positive cells were stained green, VSMCs were stained for SMαA and shown in red. Nuclei were stained blue by DAPI. The percentages of apoptotic nuclei were calculated by determining the number of DAPI-stained nuclei that were also positive for TUNEL staining. Approximately, 100 nuclei cells were counted in randomly chosen fields per region ( H ). VSMC migration was determined by a standard wound healing assay ( I ). Bar graphs represent the mean ± SEM of three independent experiments ( J ). * P
    Fitc Conjugated Secondary Antibodies, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 92/100, based on 130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Becton Dickinson fitc conjugated secondary antibody
    Elevated Viral Replication and Reduced Maturation of SJL DCs following TMEV Infection (A) For the virus binding assay, DCs obtained from a 5-d culture of primary BM cells were incubated with UV-inactivated TMEV or TMEV (MOI 10) for 1 h at 4 °C, fixed with 1% PFA, and stained with anti-TMEV monoclonal antibody (open histogram) or isotype antibody (filled histogram) followed by <t>FITC-conjugated</t> secondary antibody. (B) To determine viral infection and replication levels, DCs were infected at MOI 10 for 1, 6, 12, 24, and 48 h. Expression of TMEV proteins in DCs was detected using anti-TMEV <t>mAb</t> and anti-CD11c antibody. Virus production was assessed by plaque assay. (C) The fraction of virus protein-positive cells was determined at different stages of culture of BMCs. (D) DCs were infected for 24 h and then analyzed for levels of maturation-associated markers (MHC-II, CD86, and CCR7, y -axis) and TMEV protein ( x -axis) by intracellular staining with gated CD11c + cells.
    Fitc Conjugated Secondary Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 451 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Beyotime fitc conjugated secondary antibody
    Histology of lenses without or with dexamethasone-exposed lenses. Whole lenses were cultured for 48 h without (control) or with dexamethasone. Lens sections were stained with primary antibodies(same with western-blot experiment) then stained with a second round of antibodies <t>(HRP-conjuncted,Cy3</t> or <t>FITC-conjuncted).</t>
    Fitc Conjugated Secondary Antibody, supplied by Beyotime, used in various techniques. Bioz Stars score: 92/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co fitc conjugated secondary antibody
    Histology of lenses without or with dexamethasone-exposed lenses. Whole lenses were cultured for 48 h without (control) or with dexamethasone. Lens sections were stained with primary antibodies(same with western-blot experiment) then stained with a second round of antibodies <t>(HRP-conjuncted,Cy3</t> or <t>FITC-conjuncted).</t>
    Fitc Conjugated Secondary Antibody, supplied by Merck & Co, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Argene fitc conjugated secondary antibody
    Histology of lenses without or with dexamethasone-exposed lenses. Whole lenses were cultured for 48 h without (control) or with dexamethasone. Lens sections were stained with primary antibodies(same with western-blot experiment) then stained with a second round of antibodies <t>(HRP-conjuncted,Cy3</t> or <t>FITC-conjuncted).</t>
    Fitc Conjugated Secondary Antibody, supplied by Argene, used in various techniques. Bioz Stars score: 92/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc fitc conjugated secondary antibody
    Histology of lenses without or with dexamethasone-exposed lenses. Whole lenses were cultured for 48 h without (control) or with dexamethasone. Lens sections were stained with primary antibodies(same with western-blot experiment) then stained with a second round of antibodies <t>(HRP-conjuncted,Cy3</t> or <t>FITC-conjuncted).</t>
    Fitc Conjugated Secondary Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc conjugated secondary antibody/product/Cell Signaling Technology Inc
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    93
    Bio-Synthesis Inc fitc conjugated secondary antibody
    Histology of lenses without or with dexamethasone-exposed lenses. Whole lenses were cultured for 48 h without (control) or with dexamethasone. Lens sections were stained with primary antibodies(same with western-blot experiment) then stained with a second round of antibodies <t>(HRP-conjuncted,Cy3</t> or <t>FITC-conjuncted).</t>
    Fitc Conjugated Secondary Antibody, supplied by Bio-Synthesis Inc, used in various techniques. Bioz Stars score: 93/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc conjugated secondary antibody/product/Bio-Synthesis Inc
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    GE Healthcare fitc conjugated secondary antibody
    Histology of lenses without or with dexamethasone-exposed lenses. Whole lenses were cultured for 48 h without (control) or with dexamethasone. Lens sections were stained with primary antibodies(same with western-blot experiment) then stained with a second round of antibodies <t>(HRP-conjuncted,Cy3</t> or <t>FITC-conjuncted).</t>
    Fitc Conjugated Secondary Antibody, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc conjugated secondary antibody/product/GE Healthcare
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    89
    Valiant secondary antibodies conjugated to fitc
    Histology of lenses without or with dexamethasone-exposed lenses. Whole lenses were cultured for 48 h without (control) or with dexamethasone. Lens sections were stained with primary antibodies(same with western-blot experiment) then stained with a second round of antibodies <t>(HRP-conjuncted,Cy3</t> or <t>FITC-conjuncted).</t>
    Secondary Antibodies Conjugated To Fitc, supplied by Valiant, used in various techniques. Bioz Stars score: 89/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    PAT-SM6 specifically binds to MM cell lines and primary human MM samples. A, INA-6, NCI H929, MM1.S, OPM-2 and U266 MM cell lines or CD138-purified patient MM cells were stained with PAT-SM6 (5 µg/mL, open histogram) or isotype control (gray histogram) followed by fluorescein isothiocyanate (FITC)-conjugated secondary antibodies and assessed by flow cytometry. Both, cell lines as well as primary MM cells displayed specific PAT-SM6 binding. B, Immunohistochemistry with PAT-SM6, CD138-positive control antibody or isotype on bone marrow biopsies of MM patients. PAT-SM6 specifically stained MM cells with a homogenous binding pattern. In samples without MM infiltration no specific binding was detected. Images were captured using a Leica DM BL microscope, the Leica ICC HD digital camera and the Leica LAS EZ V2.1.0 software.

    Journal: PLoS ONE

    Article Title: The Natural Human IgM Antibody PAT-SM6 Induces Apoptosis in Primary Human Multiple Myeloma Cells by Targeting Heat Shock Protein GRP78

    doi: 10.1371/journal.pone.0063414

    Figure Lengend Snippet: PAT-SM6 specifically binds to MM cell lines and primary human MM samples. A, INA-6, NCI H929, MM1.S, OPM-2 and U266 MM cell lines or CD138-purified patient MM cells were stained with PAT-SM6 (5 µg/mL, open histogram) or isotype control (gray histogram) followed by fluorescein isothiocyanate (FITC)-conjugated secondary antibodies and assessed by flow cytometry. Both, cell lines as well as primary MM cells displayed specific PAT-SM6 binding. B, Immunohistochemistry with PAT-SM6, CD138-positive control antibody or isotype on bone marrow biopsies of MM patients. PAT-SM6 specifically stained MM cells with a homogenous binding pattern. In samples without MM infiltration no specific binding was detected. Images were captured using a Leica DM BL microscope, the Leica ICC HD digital camera and the Leica LAS EZ V2.1.0 software.

    Article Snippet: The expression of GRP78 on MM cells was assessed using anti-GRP78 IgG (rabbit), as well as PAT-SM6 followed by fluorescein isothiocyanate (FITC)-conjugated secondary antibodies (Abcam or Dako).

    Techniques: Purification, Staining, Flow Cytometry, Cytometry, Binding Assay, Immunohistochemistry, Positive Control, Microscopy, Immunocytochemistry, Software

    Selection of ASLV-B infected DF-1 cells that do not undergo low pH-mediated cell-cell fusion. (A) Selection scheme used to identify subgroup B ASLV-infected cells that are resistant to low pH-mediated syncytia formation. (B) The numbers of hygromycin B-resistant virus-infected colonies that resulted from cell-cell fusion experiments preformed with 293:TVB S3 Δ DD cells and either starting population of RCASH-B infected cells or R6 (pH 5.6) cells, are shown. This experiment was performed in triplicate and the average mean values obtained are shown along with the standard deviation of the data. (C) Flow cytometric analysis of EnvB expression. Uninfected cells (green histogram), the starting population of RCASH-B infected DF-1 cells (red histogram, upper panel) and the R6 (pH 5.6) cells (blue histogram, lower panel) were incubated with TVB S3 -rIgG and a FITC-conjugated anti-rabbit antibody and analyzed by flow cytometry as described previously [5] , [27] .

    Journal: PLoS ONE

    Article Title: The hr1 and Fusion Peptide Regions of the Subgroup B Avian Sarcoma and Leukosis Virus Envelope Glycoprotein Influence Low pH-Dependent Membrane Fusion

    doi: 10.1371/journal.pone.0000171

    Figure Lengend Snippet: Selection of ASLV-B infected DF-1 cells that do not undergo low pH-mediated cell-cell fusion. (A) Selection scheme used to identify subgroup B ASLV-infected cells that are resistant to low pH-mediated syncytia formation. (B) The numbers of hygromycin B-resistant virus-infected colonies that resulted from cell-cell fusion experiments preformed with 293:TVB S3 Δ DD cells and either starting population of RCASH-B infected cells or R6 (pH 5.6) cells, are shown. This experiment was performed in triplicate and the average mean values obtained are shown along with the standard deviation of the data. (C) Flow cytometric analysis of EnvB expression. Uninfected cells (green histogram), the starting population of RCASH-B infected DF-1 cells (red histogram, upper panel) and the R6 (pH 5.6) cells (blue histogram, lower panel) were incubated with TVB S3 -rIgG and a FITC-conjugated anti-rabbit antibody and analyzed by flow cytometry as described previously [5] , [27] .

    Article Snippet: Flow cytometry to determine EnvB surface expression was performed with a TVB-immunoadhesin (TVBS3 -IgG) and with a FITC-conjugated secondary antibody (Dako, Denmark) as described previously .

    Techniques: Selection, Infection, Standard Deviation, Flow Cytometry, Expressing, Incubation, Cytometry

    Effects of Pin1 on proliferation, cell cycle progression, apoptosis and migration in vascular smooth muscle cells (VSMCs). VSMCs were cultured and infected as described in Figure 3 . Cell proliferation was assessed by both the MTT assay ( A ) and [ 3 H]-thymidine incorporation analyses ( B ). Cell cycle analysis was quantified by PI staining followed by flow cytometry analyses. M1, M2, M3 and M4 indicate sub G1, G0/G1, S and G2/M phases respectively ( C ). Bar graphs represent the mean ± SEM of three independent experiments ( D ). Cell apoptosis was detected by annexin V-FITC/PI staining ( E ) followed by flow cytometry analysis and TUNEL staining ( G ). The proportion of live cells (annexin V−/PI−), early apoptotic cells (annexin V+/PI−), late apoptotic/necrotic cells (annexin V+/PI+) and dead cells (annexin V−/PI+) was calculated for comparison ( F ). The TUNEL-positive cells were stained green, VSMCs were stained for SMαA and shown in red. Nuclei were stained blue by DAPI. The percentages of apoptotic nuclei were calculated by determining the number of DAPI-stained nuclei that were also positive for TUNEL staining. Approximately, 100 nuclei cells were counted in randomly chosen fields per region ( H ). VSMC migration was determined by a standard wound healing assay ( I ). Bar graphs represent the mean ± SEM of three independent experiments ( J ). * P

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Essential role of Pin1 via STAT3 signalling and mitochondria-dependent pathways in restenosis in type 2 diabetes

    doi: 10.1111/jcmm.12082

    Figure Lengend Snippet: Effects of Pin1 on proliferation, cell cycle progression, apoptosis and migration in vascular smooth muscle cells (VSMCs). VSMCs were cultured and infected as described in Figure 3 . Cell proliferation was assessed by both the MTT assay ( A ) and [ 3 H]-thymidine incorporation analyses ( B ). Cell cycle analysis was quantified by PI staining followed by flow cytometry analyses. M1, M2, M3 and M4 indicate sub G1, G0/G1, S and G2/M phases respectively ( C ). Bar graphs represent the mean ± SEM of three independent experiments ( D ). Cell apoptosis was detected by annexin V-FITC/PI staining ( E ) followed by flow cytometry analysis and TUNEL staining ( G ). The proportion of live cells (annexin V−/PI−), early apoptotic cells (annexin V+/PI−), late apoptotic/necrotic cells (annexin V+/PI+) and dead cells (annexin V−/PI+) was calculated for comparison ( F ). The TUNEL-positive cells were stained green, VSMCs were stained for SMαA and shown in red. Nuclei were stained blue by DAPI. The percentages of apoptotic nuclei were calculated by determining the number of DAPI-stained nuclei that were also positive for TUNEL staining. Approximately, 100 nuclei cells were counted in randomly chosen fields per region ( H ). VSMC migration was determined by a standard wound healing assay ( I ). Bar graphs represent the mean ± SEM of three independent experiments ( J ). * P

    Article Snippet: Thereafter, cells were incubated with anti-α-actin and anti-pin1 antibody (Abcam, Cambridge, UK) and further stained with appropriate TRITC- or FITC-conjugated secondary antibodies (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA).

    Techniques: Migration, Cell Culture, Infection, MTT Assay, Cell Cycle Assay, Staining, Flow Cytometry, Cytometry, TUNEL Assay, Wound Healing Assay

    Elevated Viral Replication and Reduced Maturation of SJL DCs following TMEV Infection (A) For the virus binding assay, DCs obtained from a 5-d culture of primary BM cells were incubated with UV-inactivated TMEV or TMEV (MOI 10) for 1 h at 4 °C, fixed with 1% PFA, and stained with anti-TMEV monoclonal antibody (open histogram) or isotype antibody (filled histogram) followed by FITC-conjugated secondary antibody. (B) To determine viral infection and replication levels, DCs were infected at MOI 10 for 1, 6, 12, 24, and 48 h. Expression of TMEV proteins in DCs was detected using anti-TMEV mAb and anti-CD11c antibody. Virus production was assessed by plaque assay. (C) The fraction of virus protein-positive cells was determined at different stages of culture of BMCs. (D) DCs were infected for 24 h and then analyzed for levels of maturation-associated markers (MHC-II, CD86, and CCR7, y -axis) and TMEV protein ( x -axis) by intracellular staining with gated CD11c + cells.

    Journal: PLoS Pathogens

    Article Title: Role of Dendritic Cells in Differential Susceptibility to Viral Demyelinating Disease

    doi: 10.1371/journal.ppat.0030124

    Figure Lengend Snippet: Elevated Viral Replication and Reduced Maturation of SJL DCs following TMEV Infection (A) For the virus binding assay, DCs obtained from a 5-d culture of primary BM cells were incubated with UV-inactivated TMEV or TMEV (MOI 10) for 1 h at 4 °C, fixed with 1% PFA, and stained with anti-TMEV monoclonal antibody (open histogram) or isotype antibody (filled histogram) followed by FITC-conjugated secondary antibody. (B) To determine viral infection and replication levels, DCs were infected at MOI 10 for 1, 6, 12, 24, and 48 h. Expression of TMEV proteins in DCs was detected using anti-TMEV mAb and anti-CD11c antibody. Virus production was assessed by plaque assay. (C) The fraction of virus protein-positive cells was determined at different stages of culture of BMCs. (D) DCs were infected for 24 h and then analyzed for levels of maturation-associated markers (MHC-II, CD86, and CCR7, y -axis) and TMEV protein ( x -axis) by intracellular staining with gated CD11c + cells.

    Article Snippet: Cells were fixed, permeabilized (perm/fix solution, BD PharMingen), blocked with 1% normal goat serum (Invitrogen), and stained with 8C mAb followed by FITC-conjugated secondary antibody or PE-conjugated goat anti-mouse Ig (BD PharMingen).

    Techniques: Infection, Binding Assay, Incubation, Staining, Expressing, Plaque Assay

    Histology of lenses without or with dexamethasone-exposed lenses. Whole lenses were cultured for 48 h without (control) or with dexamethasone. Lens sections were stained with primary antibodies(same with western-blot experiment) then stained with a second round of antibodies (HRP-conjuncted,Cy3 or FITC-conjuncted).

    Journal: Molecular Vision

    Article Title: Proteomics analysis of water insoluble-urea soluble crystallins from normal and dexamethasone exposed lens

    doi:

    Figure Lengend Snippet: Histology of lenses without or with dexamethasone-exposed lenses. Whole lenses were cultured for 48 h without (control) or with dexamethasone. Lens sections were stained with primary antibodies(same with western-blot experiment) then stained with a second round of antibodies (HRP-conjuncted,Cy3 or FITC-conjuncted).

    Article Snippet: Afterwards, samples were washed with Tris-buffered saline then incubated for 1 h with Cy3-conjugated or FITC-conjugated secondary antibody (1:100; Beyotime Institute of Biotechnology).

    Techniques: Cell Culture, Staining, Western Blot