first-strand reverse transcription rt primer Search Results


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    Transcriptional induction of the STX2 gene in STEC strains O157:H7 and O104:H4 by various antibiotics. STEC strains O157:H7 and O104:H4 were inoculated into L-broth at a density of 1x10 8 bacteria/ml. The cultures were either left without antibiotics or treated immediately with the indicated n-folds of the MIC of the indicated antibiotics and incubated at 37°C under vigorous shaking. After 2 h, 200 μl of the bacterial suspensions were harvested to prepare total RNA and to determine by <t>qRT-PCR</t> the numbers of STX2-specific transcripts. Green or red columns highlight the values after treatment with the 1-fold or 4-fold MIC, respectively. This colour code is used throughout the manuscript. Shown are the means and standard errors of <t>three</t> independent experiments. Statistical significance is indicated by asterisks: * for p
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    Transcriptional induction of the STX2 gene in STEC strains O157:H7 and O104:H4 by various antibiotics. STEC strains O157:H7 and O104:H4 were inoculated into L-broth at a density of 1x10 8 bacteria/ml. The cultures were either left without antibiotics or treated immediately with the indicated n-folds of the MIC of the indicated antibiotics and incubated at 37°C under vigorous shaking. After 2 h, 200 μl of the bacterial suspensions were harvested to prepare total RNA and to determine by <t>qRT-PCR</t> the numbers of STX2-specific transcripts. Green or red columns highlight the values after treatment with the 1-fold or 4-fold MIC, respectively. This colour code is used throughout the manuscript. Shown are the means and standard errors of <t>three</t> independent experiments. Statistical significance is indicated by asterisks: * for p
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    Transcriptional induction of the STX2 gene in STEC strains O157:H7 and O104:H4 by various antibiotics. STEC strains O157:H7 and O104:H4 were inoculated into L-broth at a density of 1x10 8 bacteria/ml. The cultures were either left without antibiotics or treated immediately with the indicated n-folds of the MIC of the indicated antibiotics and incubated at 37°C under vigorous shaking. After 2 h, 200 μl of the bacterial suspensions were harvested to prepare total RNA and to determine by <t>qRT-PCR</t> the numbers of STX2-specific transcripts. Green or red columns highlight the values after treatment with the 1-fold or 4-fold MIC, respectively. This colour code is used throughout the manuscript. Shown are the means and standard errors of <t>three</t> independent experiments. Statistical significance is indicated by asterisks: * for p
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    Transcriptional induction of the STX2 gene in STEC strains O157:H7 and O104:H4 by various antibiotics. STEC strains O157:H7 and O104:H4 were inoculated into L-broth at a density of 1x10 8 bacteria/ml. The cultures were either left without antibiotics or treated immediately with the indicated n-folds of the MIC of the indicated antibiotics and incubated at 37°C under vigorous shaking. After 2 h, 200 μl of the bacterial suspensions were harvested to prepare total RNA and to determine by <t>qRT-PCR</t> the numbers of STX2-specific transcripts. Green or red columns highlight the values after treatment with the 1-fold or 4-fold MIC, respectively. This colour code is used throughout the manuscript. Shown are the means and standard errors of <t>three</t> independent experiments. Statistical significance is indicated by asterisks: * for p
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    Transcriptional induction of the STX2 gene in STEC strains O157:H7 and O104:H4 by various antibiotics. STEC strains O157:H7 and O104:H4 were inoculated into L-broth at a density of 1x10 8 bacteria/ml. The cultures were either left without antibiotics or treated immediately with the indicated n-folds of the MIC of the indicated antibiotics and incubated at 37°C under vigorous shaking. After 2 h, 200 μl of the bacterial suspensions were harvested to prepare total RNA and to determine by <t>qRT-PCR</t> the numbers of STX2-specific transcripts. Green or red columns highlight the values after treatment with the 1-fold or 4-fold MIC, respectively. This colour code is used throughout the manuscript. Shown are the means and standard errors of <t>three</t> independent experiments. Statistical significance is indicated by asterisks: * for p
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    Transcriptional induction of the STX2 gene in STEC strains O157:H7 and O104:H4 by various antibiotics. STEC strains O157:H7 and O104:H4 were inoculated into L-broth at a density of 1x10 8 bacteria/ml. The cultures were either left without antibiotics or treated immediately with the indicated n-folds of the MIC of the indicated antibiotics and incubated at 37°C under vigorous shaking. After 2 h, 200 μl of the bacterial suspensions were harvested to prepare total RNA and to determine by <t>qRT-PCR</t> the numbers of STX2-specific transcripts. Green or red columns highlight the values after treatment with the 1-fold or 4-fold MIC, respectively. This colour code is used throughout the manuscript. Shown are the means and standard errors of <t>three</t> independent experiments. Statistical significance is indicated by asterisks: * for p
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    Transcriptional induction of the STX2 gene in STEC strains O157:H7 and O104:H4 by various antibiotics. STEC strains O157:H7 and O104:H4 were inoculated into L-broth at a density of 1x10 8 bacteria/ml. The cultures were either left without antibiotics or treated immediately with the indicated n-folds of the MIC of the indicated antibiotics and incubated at 37°C under vigorous shaking. After 2 h, 200 μl of the bacterial suspensions were harvested to prepare total RNA and to determine by <t>qRT-PCR</t> the numbers of STX2-specific transcripts. Green or red columns highlight the values after treatment with the 1-fold or 4-fold MIC, respectively. This colour code is used throughout the manuscript. Shown are the means and standard errors of <t>three</t> independent experiments. Statistical significance is indicated by asterisks: * for p
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    Transcriptional induction of the STX2 gene in STEC strains O157:H7 and O104:H4 by various antibiotics. STEC strains O157:H7 and O104:H4 were inoculated into L-broth at a density of 1x10 8 bacteria/ml. The cultures were either left without antibiotics or treated immediately with the indicated n-folds of the MIC of the indicated antibiotics and incubated at 37°C under vigorous shaking. After 2 h, 200 μl of the bacterial suspensions were harvested to prepare total RNA and to determine by qRT-PCR the numbers of STX2-specific transcripts. Green or red columns highlight the values after treatment with the 1-fold or 4-fold MIC, respectively. This colour code is used throughout the manuscript. Shown are the means and standard errors of three independent experiments. Statistical significance is indicated by asterisks: * for p

    Journal: BMC Microbiology

    Article Title: Therapeutic concentrations of antibiotics inhibit Shiga toxin release from enterohemorrhagic E. coli O104:H4 from the 2011 German outbreak

    doi: 10.1186/1471-2180-12-160

    Figure Lengend Snippet: Transcriptional induction of the STX2 gene in STEC strains O157:H7 and O104:H4 by various antibiotics. STEC strains O157:H7 and O104:H4 were inoculated into L-broth at a density of 1x10 8 bacteria/ml. The cultures were either left without antibiotics or treated immediately with the indicated n-folds of the MIC of the indicated antibiotics and incubated at 37°C under vigorous shaking. After 2 h, 200 μl of the bacterial suspensions were harvested to prepare total RNA and to determine by qRT-PCR the numbers of STX2-specific transcripts. Green or red columns highlight the values after treatment with the 1-fold or 4-fold MIC, respectively. This colour code is used throughout the manuscript. Shown are the means and standard errors of three independent experiments. Statistical significance is indicated by asterisks: * for p

    Article Snippet: An 8 μl aliquot of each RNA extraction was transcribed into cDNA using random hexamers as primer according to the manufacturer’s instructions (SuperScript III First-Strand Synthesis System for RT-PCR, Invitrogen). cDNA was stored at −20°C until further use.

    Techniques: Incubation, Quantitative RT-PCR