first-strand cdna templates Search Results


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  • 99
    Thermo Fisher first strand cdna template
    A , electrophoresis of PCR ( a – c ) and Northern blot hybridization ( d ). Lane 1 , electrophoresis results of RT-PCR in the human, orangutan, and rhesus <t>cDNA</t> libraries, respectively, using a pair of primers spanning the coding region. Lane M , DNA size marker ladder (Invitrogen, 1-kb DNA size ladder). d , Northern blot of total <t>RNA</t> from bovine ( lane 1 ) or rhesus spleen tissue ( lane 2 ) hybridized with a probe derived from rhesus exon 9 fragment. As expected, with in-species versus cross-species hybridization, gene expression was lower in the bovine than in the monkey spleen cells. B, schematic splicing variations and cryptic exons of human ( a ), orangutan ( b ), and rhesus ( c ) α 1,3GT gene. The closed boxes denote exons in the respective species. The open boxes with arrows denote cryptic exons that were not observed in the α Gal-positive species. Exon 6Rc in rhesus has exon 6, intron 6, and exon 7 (a retained intron).
    First Strand Cdna Template, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9777 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    first strand cdna template - by Bioz Stars, 2020-08
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    97
    Millipore first strand cdna template
    Identification of an RNA editing fingerprint of malignant progenitor reprogramming, and stable ADAR1 overexpression in K562 cells. (A) LSC purification strategy for detection of CSC-associated RNA recoding. (B-D) RNA-sequencing analysis of FACS-purified CP and BC CML LSC [ 1 ] showing A-to-G RNA editing changes in MDM2, AZIN1 and APOBEC3D (n = 8 per group). (E) Lentiviral construct expressing human ADAR1 or a catalytically inactive form (ADAR1m). (F-H) <t>qRT-PCR</t> analysis of <t>cDNA</t> prepared from K562 lines using primers detecting ADAR1 lentivirus (F) and total human ADAR1 (G,H) showing K562 leukemia cells stably transduced with active ADAR1 or inactive ADAR1m express high levels of ADAR1 transcripts compared with vector open reading frame (ORF) control backbone. *p
    First Strand Cdna Template, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Bio-Rad first strand complementary dna cdna template
    Identification of an RNA editing fingerprint of malignant progenitor reprogramming, and stable ADAR1 overexpression in K562 cells. (A) LSC purification strategy for detection of CSC-associated RNA recoding. (B-D) RNA-sequencing analysis of FACS-purified CP and BC CML LSC [ 1 ] showing A-to-G RNA editing changes in MDM2, AZIN1 and APOBEC3D (n = 8 per group). (E) Lentiviral construct expressing human ADAR1 or a catalytically inactive form (ADAR1m). (F-H) <t>qRT-PCR</t> analysis of <t>cDNA</t> prepared from K562 lines using primers detecting ADAR1 lentivirus (F) and total human ADAR1 (G,H) showing K562 leukemia cells stably transduced with active ADAR1 or inactive ADAR1m express high levels of ADAR1 transcripts compared with vector open reading frame (ORF) control backbone. *p
    First Strand Complementary Dna Cdna Template, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    TaKaRa first strand cdna template
    Expression analysis of CarF-box_PP2 and CarF-box_LysM . A) Northern-blot analysis of the total RNA isolated from fully-opened flower and developing seeds (10 DAA, 20 DAA, 30 DAA and 40 DAA) of chickpea. Full-length CarF-box_PP2 and CarF-box_LysM <t>cDNA</t> were used as probes. EF1α was used to reprobe the blot. B) Semi-quantitative <t>PCR</t> analysis. The cDNA was amplified using gene-specific primers for CarF-box_PP2 , CarF-box_LysM and EF1α.
    First Strand Cdna Template, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 242 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Roche first strand cdna template
    Expression analysis of CarF-box_PP2 and CarF-box_LysM . A) Northern-blot analysis of the total RNA isolated from fully-opened flower and developing seeds (10 DAA, 20 DAA, 30 DAA and 40 DAA) of chickpea. Full-length CarF-box_PP2 and CarF-box_LysM <t>cDNA</t> were used as probes. EF1α was used to reprobe the blot. B) Semi-quantitative <t>PCR</t> analysis. The cDNA was amplified using gene-specific primers for CarF-box_PP2 , CarF-box_LysM and EF1α.
    First Strand Cdna Template, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad first strand cdna template
    Expression analysis of CarF-box_PP2 and CarF-box_LysM . A) Northern-blot analysis of the total RNA isolated from fully-opened flower and developing seeds (10 DAA, 20 DAA, 30 DAA and 40 DAA) of chickpea. Full-length CarF-box_PP2 and CarF-box_LysM <t>cDNA</t> were used as probes. EF1α was used to reprobe the blot. B) Semi-quantitative <t>PCR</t> analysis. The cDNA was amplified using gene-specific primers for CarF-box_PP2 , CarF-box_LysM and EF1α.
    First Strand Cdna Template, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche cdna first strand template
    Expression analysis of CarF-box_PP2 and CarF-box_LysM . A) Northern-blot analysis of the total RNA isolated from fully-opened flower and developing seeds (10 DAA, 20 DAA, 30 DAA and 40 DAA) of chickpea. Full-length CarF-box_PP2 and CarF-box_LysM <t>cDNA</t> were used as probes. EF1α was used to reprobe the blot. B) Semi-quantitative <t>PCR</t> analysis. The cDNA was amplified using gene-specific primers for CarF-box_PP2 , CarF-box_LysM and EF1α.
    Cdna First Strand Template, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Toyobo cdna first strand template
    Expression analysis of CarF-box_PP2 and CarF-box_LysM . A) Northern-blot analysis of the total RNA isolated from fully-opened flower and developing seeds (10 DAA, 20 DAA, 30 DAA and 40 DAA) of chickpea. Full-length CarF-box_PP2 and CarF-box_LysM <t>cDNA</t> were used as probes. EF1α was used to reprobe the blot. B) Semi-quantitative <t>PCR</t> analysis. The cDNA was amplified using gene-specific primers for CarF-box_PP2 , CarF-box_LysM and EF1α.
    Cdna First Strand Template, supplied by Toyobo, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    ABM Inc first strand complementary dna cdna
    Expression analysis of CarF-box_PP2 and CarF-box_LysM . A) Northern-blot analysis of the total RNA isolated from fully-opened flower and developing seeds (10 DAA, 20 DAA, 30 DAA and 40 DAA) of chickpea. Full-length CarF-box_PP2 and CarF-box_LysM <t>cDNA</t> were used as probes. EF1α was used to reprobe the blot. B) Semi-quantitative <t>PCR</t> analysis. The cDNA was amplified using gene-specific primers for CarF-box_PP2 , CarF-box_LysM and EF1α.
    First Strand Complementary Dna Cdna, supplied by ABM Inc, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    GE Healthcare strand complementary dna cdna synthesis
    Expression analysis of CarF-box_PP2 and CarF-box_LysM . A) Northern-blot analysis of the total RNA isolated from fully-opened flower and developing seeds (10 DAA, 20 DAA, 30 DAA and 40 DAA) of chickpea. Full-length CarF-box_PP2 and CarF-box_LysM <t>cDNA</t> were used as probes. EF1α was used to reprobe the blot. B) Semi-quantitative <t>PCR</t> analysis. The cDNA was amplified using gene-specific primers for CarF-box_PP2 , CarF-box_LysM and EF1α.
    Strand Complementary Dna Cdna Synthesis, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher first strand complementary dna cdna synthesis
    Expression analysis of CarF-box_PP2 and CarF-box_LysM . A) Northern-blot analysis of the total RNA isolated from fully-opened flower and developing seeds (10 DAA, 20 DAA, 30 DAA and 40 DAA) of chickpea. Full-length CarF-box_PP2 and CarF-box_LysM <t>cDNA</t> were used as probes. EF1α was used to reprobe the blot. B) Semi-quantitative <t>PCR</t> analysis. The cDNA was amplified using gene-specific primers for CarF-box_PP2 , CarF-box_LysM and EF1α.
    First Strand Complementary Dna Cdna Synthesis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 663 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Bio-Rad first strand complementary dna cdna synthesis
    Expression analysis of CarF-box_PP2 and CarF-box_LysM . A) Northern-blot analysis of the total RNA isolated from fully-opened flower and developing seeds (10 DAA, 20 DAA, 30 DAA and 40 DAA) of chickpea. Full-length CarF-box_PP2 and CarF-box_LysM <t>cDNA</t> were used as probes. EF1α was used to reprobe the blot. B) Semi-quantitative <t>PCR</t> analysis. The cDNA was amplified using gene-specific primers for CarF-box_PP2 , CarF-box_LysM and EF1α.
    First Strand Complementary Dna Cdna Synthesis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher first strand mirna cdna pcr template
    Flow diagram of participant screen and <t>EPS-miRNA</t> identification The procedure for identifying EPS-miRNAs consisted of four phages, including patient screen phage, discovery phage, testing phage, and validation phage. In screen phage, 60 healthy men and 59 IIIACP/CPPS patients with significant prostatitis-like pain (NIH-CPSI pain score ≥ 10) were finally included primary screen, symptom assessment, and EPS examination. In discovery phage, high-throughput sequencing was employed to identify characteristic expression-profile of EPS-miRNAs for healthy men and IIIA CP/CPPS patients. In testing phage, elevated levels of identified EPS-miRNAs were further confirmed in the individual EPS samples from 33 patients by comparing to 30 healthy men with Taqman-based <t>qRT-PCR.</t> In validation phage, the change levels of top dysregulated EPS-miRNAs were measured traceably in 21 follow-up patients, and their classify-accuracy on IIIA CP/CPPS were subsequently analyzed by ROC curve.
    First Strand Mirna Cdna Pcr Template, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher strand complementary dna synthesis reaction
    Flow diagram of participant screen and <t>EPS-miRNA</t> identification The procedure for identifying EPS-miRNAs consisted of four phages, including patient screen phage, discovery phage, testing phage, and validation phage. In screen phage, 60 healthy men and 59 IIIACP/CPPS patients with significant prostatitis-like pain (NIH-CPSI pain score ≥ 10) were finally included primary screen, symptom assessment, and EPS examination. In discovery phage, high-throughput sequencing was employed to identify characteristic expression-profile of EPS-miRNAs for healthy men and IIIA CP/CPPS patients. In testing phage, elevated levels of identified EPS-miRNAs were further confirmed in the individual EPS samples from 33 patients by comparing to 30 healthy men with Taqman-based <t>qRT-PCR.</t> In validation phage, the change levels of top dysregulated EPS-miRNAs were measured traceably in 21 follow-up patients, and their classify-accuracy on IIIA CP/CPPS were subsequently analyzed by ROC curve.
    Strand Complementary Dna Synthesis Reaction, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Millipore cdna synthesis
    Flow diagram of participant screen and <t>EPS-miRNA</t> identification The procedure for identifying EPS-miRNAs consisted of four phages, including patient screen phage, discovery phage, testing phage, and validation phage. In screen phage, 60 healthy men and 59 IIIACP/CPPS patients with significant prostatitis-like pain (NIH-CPSI pain score ≥ 10) were finally included primary screen, symptom assessment, and EPS examination. In discovery phage, high-throughput sequencing was employed to identify characteristic expression-profile of EPS-miRNAs for healthy men and IIIA CP/CPPS patients. In testing phage, elevated levels of identified EPS-miRNAs were further confirmed in the individual EPS samples from 33 patients by comparing to 30 healthy men with Taqman-based <t>qRT-PCR.</t> In validation phage, the change levels of top dysregulated EPS-miRNAs were measured traceably in 21 follow-up patients, and their classify-accuracy on IIIA CP/CPPS were subsequently analyzed by ROC curve.
    Cdna Synthesis, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 2592 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher strand cdna
    Flow diagram of participant screen and <t>EPS-miRNA</t> identification The procedure for identifying EPS-miRNAs consisted of four phages, including patient screen phage, discovery phage, testing phage, and validation phage. In screen phage, 60 healthy men and 59 IIIACP/CPPS patients with significant prostatitis-like pain (NIH-CPSI pain score ≥ 10) were finally included primary screen, symptom assessment, and EPS examination. In discovery phage, high-throughput sequencing was employed to identify characteristic expression-profile of EPS-miRNAs for healthy men and IIIA CP/CPPS patients. In testing phage, elevated levels of identified EPS-miRNAs were further confirmed in the individual EPS samples from 33 patients by comparing to 30 healthy men with Taqman-based <t>qRT-PCR.</t> In validation phage, the change levels of top dysregulated EPS-miRNAs were measured traceably in 21 follow-up patients, and their classify-accuracy on IIIA CP/CPPS were subsequently analyzed by ROC curve.
    Strand Cdna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 9683 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    A , electrophoresis of PCR ( a – c ) and Northern blot hybridization ( d ). Lane 1 , electrophoresis results of RT-PCR in the human, orangutan, and rhesus cDNA libraries, respectively, using a pair of primers spanning the coding region. Lane M , DNA size marker ladder (Invitrogen, 1-kb DNA size ladder). d , Northern blot of total RNA from bovine ( lane 1 ) or rhesus spleen tissue ( lane 2 ) hybridized with a probe derived from rhesus exon 9 fragment. As expected, with in-species versus cross-species hybridization, gene expression was lower in the bovine than in the monkey spleen cells. B, schematic splicing variations and cryptic exons of human ( a ), orangutan ( b ), and rhesus ( c ) α 1,3GT gene. The closed boxes denote exons in the respective species. The open boxes with arrows denote cryptic exons that were not observed in the α Gal-positive species. Exon 6Rc in rhesus has exon 6, intron 6, and exon 7 (a retained intron).

    Journal: The Journal of biological chemistry

    Article Title: Molecular Basis of Evolutionary Loss of the α1,3-Galactosyltransferase Gene in Higher Primates *

    doi: 10.1074/jbc.M110527200

    Figure Lengend Snippet: A , electrophoresis of PCR ( a – c ) and Northern blot hybridization ( d ). Lane 1 , electrophoresis results of RT-PCR in the human, orangutan, and rhesus cDNA libraries, respectively, using a pair of primers spanning the coding region. Lane M , DNA size marker ladder (Invitrogen, 1-kb DNA size ladder). d , Northern blot of total RNA from bovine ( lane 1 ) or rhesus spleen tissue ( lane 2 ) hybridized with a probe derived from rhesus exon 9 fragment. As expected, with in-species versus cross-species hybridization, gene expression was lower in the bovine than in the monkey spleen cells. B, schematic splicing variations and cryptic exons of human ( a ), orangutan ( b ), and rhesus ( c ) α 1,3GT gene. The closed boxes denote exons in the respective species. The open boxes with arrows denote cryptic exons that were not observed in the α Gal-positive species. Exon 6Rc in rhesus has exon 6, intron 6, and exon 7 (a retained intron).

    Article Snippet: Using total RNA derived from two rhesus monkeys, first-strand cDNA templates for RT-PCR were generated with the Super-Script Preamplification™ system (Invitrogen) according to the manufacturer’s protocol.

    Techniques: Electrophoresis, Polymerase Chain Reaction, Northern Blot, Hybridization, Reverse Transcription Polymerase Chain Reaction, Marker, Derivative Assay, Expressing

    TLR4 mRNA expression declines along tissues of the female reproductive tract. cDNA was synthesized by reverse transcription from total RNA, and relative TLR4 mRNA levels were assessed by real-time PCR analysis with normalization to β-actin controls. Each reaction was performed in duplicate. Comparisons among tissue types were made with paired tissue samples of the fallopian tube (FT), endometrium (EM), cervix (CX), and ectocervix (ECX) derived from the same patients. The sample size for each comparison is indicated in the upper right quadrant of each panel.

    Journal: Infection and Immunity

    Article Title: Differential Expression of Toll-Like Receptors 2 and 4 in Tissues of the Human Female Reproductive Tract

    doi: 10.1128/IAI.72.10.5799-5806.2004

    Figure Lengend Snippet: TLR4 mRNA expression declines along tissues of the female reproductive tract. cDNA was synthesized by reverse transcription from total RNA, and relative TLR4 mRNA levels were assessed by real-time PCR analysis with normalization to β-actin controls. Each reaction was performed in duplicate. Comparisons among tissue types were made with paired tissue samples of the fallopian tube (FT), endometrium (EM), cervix (CX), and ectocervix (ECX) derived from the same patients. The sample size for each comparison is indicated in the upper right quadrant of each panel.

    Article Snippet: With 500 ng of RNA as the template for each reaction, first-strand cDNA was synthesized with random primers and SuperScript II Moloney murine leukemia virus reverse transcriptase (Invitrogen).

    Techniques: Expressing, Synthesized, Real-time Polymerase Chain Reaction, Derivative Assay

    Identification of an RNA editing fingerprint of malignant progenitor reprogramming, and stable ADAR1 overexpression in K562 cells. (A) LSC purification strategy for detection of CSC-associated RNA recoding. (B-D) RNA-sequencing analysis of FACS-purified CP and BC CML LSC [ 1 ] showing A-to-G RNA editing changes in MDM2, AZIN1 and APOBEC3D (n = 8 per group). (E) Lentiviral construct expressing human ADAR1 or a catalytically inactive form (ADAR1m). (F-H) qRT-PCR analysis of cDNA prepared from K562 lines using primers detecting ADAR1 lentivirus (F) and total human ADAR1 (G,H) showing K562 leukemia cells stably transduced with active ADAR1 or inactive ADAR1m express high levels of ADAR1 transcripts compared with vector open reading frame (ORF) control backbone. *p

    Journal: Journal of Translational Medicine

    Article Title: An RNA editing fingerprint of cancer stem cell reprogramming

    doi: 10.1186/s12967-014-0370-3

    Figure Lengend Snippet: Identification of an RNA editing fingerprint of malignant progenitor reprogramming, and stable ADAR1 overexpression in K562 cells. (A) LSC purification strategy for detection of CSC-associated RNA recoding. (B-D) RNA-sequencing analysis of FACS-purified CP and BC CML LSC [ 1 ] showing A-to-G RNA editing changes in MDM2, AZIN1 and APOBEC3D (n = 8 per group). (E) Lentiviral construct expressing human ADAR1 or a catalytically inactive form (ADAR1m). (F-H) qRT-PCR analysis of cDNA prepared from K562 lines using primers detecting ADAR1 lentivirus (F) and total human ADAR1 (G,H) showing K562 leukemia cells stably transduced with active ADAR1 or inactive ADAR1m express high levels of ADAR1 transcripts compared with vector open reading frame (ORF) control backbone. *p

    Article Snippet: High-fidelity PCR and Sanger sequencing analysis For PCR and targeted Sanger sequencing analysis, 1-2 μL of first-strand cDNA templates were prepared for PCR in 25-50 μL reaction volumes using the high-fidelity KOD Hot Start DNA Polymerase kit according to the manufacturer’s instructions (EMD Millipore, Temecula, CA).

    Techniques: Over Expression, Purification, RNA Sequencing Assay, FACS, Construct, Expressing, Quantitative RT-PCR, Stable Transfection, Transduction, Plasmid Preparation

    Expression analysis of CarF-box_PP2 and CarF-box_LysM . A) Northern-blot analysis of the total RNA isolated from fully-opened flower and developing seeds (10 DAA, 20 DAA, 30 DAA and 40 DAA) of chickpea. Full-length CarF-box_PP2 and CarF-box_LysM cDNA were used as probes. EF1α was used to reprobe the blot. B) Semi-quantitative PCR analysis. The cDNA was amplified using gene-specific primers for CarF-box_PP2 , CarF-box_LysM and EF1α.

    Journal: PLoS ONE

    Article Title: A New Set of ESTs from Chickpea (Cicer arietinum L.) Embryo Reveals Two Novel F-Box Genes, CarF-box_PP2 and CarF-box_LysM, with Potential Roles in Seed Development

    doi: 10.1371/journal.pone.0121100

    Figure Lengend Snippet: Expression analysis of CarF-box_PP2 and CarF-box_LysM . A) Northern-blot analysis of the total RNA isolated from fully-opened flower and developing seeds (10 DAA, 20 DAA, 30 DAA and 40 DAA) of chickpea. Full-length CarF-box_PP2 and CarF-box_LysM cDNA were used as probes. EF1α was used to reprobe the blot. B) Semi-quantitative PCR analysis. The cDNA was amplified using gene-specific primers for CarF-box_PP2 , CarF-box_LysM and EF1α.

    Article Snippet: The PCR reaction (50 μl) contained 2 μl first-strand cDNA template, 5 μl 10x Titanium Taq PCR buffer (Clontech), 0.5 μM of each primer, 0.25 mM of dNTPs and 0.2 μl of 50X Titanium Taq DNA polymerase (Clontech).

    Techniques: Expressing, Northern Blot, Isolation, Real-time Polymerase Chain Reaction, Amplification

    Flow diagram of participant screen and EPS-miRNA identification The procedure for identifying EPS-miRNAs consisted of four phages, including patient screen phage, discovery phage, testing phage, and validation phage. In screen phage, 60 healthy men and 59 IIIACP/CPPS patients with significant prostatitis-like pain (NIH-CPSI pain score ≥ 10) were finally included primary screen, symptom assessment, and EPS examination. In discovery phage, high-throughput sequencing was employed to identify characteristic expression-profile of EPS-miRNAs for healthy men and IIIA CP/CPPS patients. In testing phage, elevated levels of identified EPS-miRNAs were further confirmed in the individual EPS samples from 33 patients by comparing to 30 healthy men with Taqman-based qRT-PCR. In validation phage, the change levels of top dysregulated EPS-miRNAs were measured traceably in 21 follow-up patients, and their classify-accuracy on IIIA CP/CPPS were subsequently analyzed by ROC curve.

    Journal: Oncotarget

    Article Title: Expression profile of microRNAs in expressed prostatic secretion of healthy men and patients with IIIA chronic prostatitis/chronic pelvic pain syndrome

    doi: 10.18632/oncotarget.24069

    Figure Lengend Snippet: Flow diagram of participant screen and EPS-miRNA identification The procedure for identifying EPS-miRNAs consisted of four phages, including patient screen phage, discovery phage, testing phage, and validation phage. In screen phage, 60 healthy men and 59 IIIACP/CPPS patients with significant prostatitis-like pain (NIH-CPSI pain score ≥ 10) were finally included primary screen, symptom assessment, and EPS examination. In discovery phage, high-throughput sequencing was employed to identify characteristic expression-profile of EPS-miRNAs for healthy men and IIIA CP/CPPS patients. In testing phage, elevated levels of identified EPS-miRNAs were further confirmed in the individual EPS samples from 33 patients by comparing to 30 healthy men with Taqman-based qRT-PCR. In validation phage, the change levels of top dysregulated EPS-miRNAs were measured traceably in 21 follow-up patients, and their classify-accuracy on IIIA CP/CPPS were subsequently analyzed by ROC curve.

    Article Snippet: The first-strand miRNA-cDNA PCR template was generated from total RNA using the TaqMan® MicroRNA Reverse Transcription Kit (Applied Biosystems), including an artificial RNA spike-in (cel-mir-39) as loading control [ , ].

    Techniques: Flow Cytometry, Next-Generation Sequencing, Expressing, Quantitative RT-PCR