first-strand cdna synthesis Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs protoscript firststrand cdna kit
    Protoscript Firststrand Cdna Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protoscript firststrand cdna kit/product/New England Biolabs
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    protoscript firststrand cdna kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher firststrand cdna synthesis kit
    <t>Dectin-1</t> silencing. DCs were electroporated with Dectin-1 siRNA or non-silencing siRNA and were cultured for 24 h at 37°C. RNA was isolated from 1 × 10 6 cells and was converted to <t>cDNA.</t> RT-PCR was performed to quantify Dectin-1 downregulation on mRNA level. Dectin-1 mRNA expression level of Dectin-1 silenced DCs was calculated compared to a non-silencing control. Data are represented as mean + SEM of n = 5 independent experiments. A student's t -test was performed and significant differences are marked with an asterisk (*** p
    Firststrand Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4221 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/firststrand cdna synthesis kit/product/Thermo Fisher
    Average 99 stars, based on 4221 article reviews
    Price from $9.99 to $1999.99
    firststrand cdna synthesis kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher revertaid firststrand cdna synthesis kit
    IL-37 + T-cells play a protective role in DSS-induced chronic colitis in mice. ( A ) Relative weight curves of WT mice, DSS-induced WT mice, DSS-induced WT mice treated with PBS, and DSS-induced WT mice injected with IL-37 + T-cells in chronic colitis. The statistical significance between the relative weights of DSS-induced mice injected with IL-37 + T-cells versus DSS-induced mice treated with PBS at the end of the experiment is shown. ( B ) The disease activity index (DAI) was determined at day 63 of the study period, based on body weight loss (0, none; 1, 1–5%; 2, 5–10%; 3, 10–20%; 4, > 20%), stool consistency (0, normal; 2, loose stool; 4, diarrhea), and stool blood (0, negative; 2, fecal occult blood test positive; 4, gross bleeding). ( C ) Representative pictures of the colon from indicated treatment cohorts. ( D ) Macroscopic damage score of the colon in the four groups. ( E ) Representative haematoxylin and eosin (H E) staining of colon sections of mice. ( F ) Histological inflammation score. This score comprises the sum of architecture of the bowel, infiltration of neutrophils and mononuclear cells, gobleT-cell depletion, and epithelial cell erosion. Three sections per animal were evaluated. ( G ) Expressions of IFN-γ, IL-1β, TNF-α, and IL-10 mRNAs within colonic tissues in the four groups. Total RNA with the RNAiso Plusi Kit. cDNAs were synthesized by using the <t>RevertAid</t> First Strand <t>cDNA</t> Synthesis Kit according to the manufacturer’s instructions. qPCR amplification reactions were prepared with the SYBR Green PCR Kit and performed using the CFX96 Real-Time System. Data are means ± SEM ( n = 6). * p
    Revertaid Firststrand Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2048 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/revertaid firststrand cdna synthesis kit/product/Thermo Fisher
    Average 99 stars, based on 2048 article reviews
    Price from $9.99 to $1999.99
    revertaid firststrand cdna synthesis kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher first strand complementary dna cdna synthesis kit
    IL-37 + T-cells play a protective role in DSS-induced chronic colitis in mice. ( A ) Relative weight curves of WT mice, DSS-induced WT mice, DSS-induced WT mice treated with PBS, and DSS-induced WT mice injected with IL-37 + T-cells in chronic colitis. The statistical significance between the relative weights of DSS-induced mice injected with IL-37 + T-cells versus DSS-induced mice treated with PBS at the end of the experiment is shown. ( B ) The disease activity index (DAI) was determined at day 63 of the study period, based on body weight loss (0, none; 1, 1–5%; 2, 5–10%; 3, 10–20%; 4, > 20%), stool consistency (0, normal; 2, loose stool; 4, diarrhea), and stool blood (0, negative; 2, fecal occult blood test positive; 4, gross bleeding). ( C ) Representative pictures of the colon from indicated treatment cohorts. ( D ) Macroscopic damage score of the colon in the four groups. ( E ) Representative haematoxylin and eosin (H E) staining of colon sections of mice. ( F ) Histological inflammation score. This score comprises the sum of architecture of the bowel, infiltration of neutrophils and mononuclear cells, gobleT-cell depletion, and epithelial cell erosion. Three sections per animal were evaluated. ( G ) Expressions of IFN-γ, IL-1β, TNF-α, and IL-10 mRNAs within colonic tissues in the four groups. Total RNA with the RNAiso Plusi Kit. cDNAs were synthesized by using the <t>RevertAid</t> First Strand <t>cDNA</t> Synthesis Kit according to the manufacturer’s instructions. qPCR amplification reactions were prepared with the SYBR Green PCR Kit and performed using the CFX96 Real-Time System. Data are means ± SEM ( n = 6). * p
    First Strand Complementary Dna Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand complementary dna cdna synthesis kit/product/Thermo Fisher
    Average 99 stars, based on 89 article reviews
    Price from $9.99 to $1999.99
    first strand complementary dna cdna synthesis kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher cdna
    Decreased Egr-1 expression in kidneys from Fli-1 +/− NZM2410 mice compared to wild-type controls. Total <t>RNA</t> was prepared from kidneys at the age of 18 weeks ( n = 4 in each group). Total RNA was converted to <t>cDNA</t> with the SuperScript First-Strand
    Cdna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 160058 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cdna/product/Thermo Fisher
    Average 99 stars, based on 160058 article reviews
    Price from $9.99 to $1999.99
    cdna - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    88
    Roche first strand complementary dna cdna synthesis kit
    Decreased Egr-1 expression in kidneys from Fli-1 +/− NZM2410 mice compared to wild-type controls. Total <t>RNA</t> was prepared from kidneys at the age of 18 weeks ( n = 4 in each group). Total RNA was converted to <t>cDNA</t> with the SuperScript First-Strand
    First Strand Complementary Dna Cdna Synthesis Kit, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand complementary dna cdna synthesis kit/product/Roche
    Average 88 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    first strand complementary dna cdna synthesis kit - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    85
    OriGene first strand complementary dna cdna synthesis system
    Decreased Egr-1 expression in kidneys from Fli-1 +/− NZM2410 mice compared to wild-type controls. Total <t>RNA</t> was prepared from kidneys at the age of 18 weeks ( n = 4 in each group). Total RNA was converted to <t>cDNA</t> with the SuperScript First-Strand
    First Strand Complementary Dna Cdna Synthesis System, supplied by OriGene, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand complementary dna cdna synthesis system/product/OriGene
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    first strand complementary dna cdna synthesis system - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    88
    Roche transcriptor first strand complementary dna cdna synthesis kit
    Decreased Egr-1 expression in kidneys from Fli-1 +/− NZM2410 mice compared to wild-type controls. Total <t>RNA</t> was prepared from kidneys at the age of 18 weeks ( n = 4 in each group). Total RNA was converted to <t>cDNA</t> with the SuperScript First-Strand
    Transcriptor First Strand Complementary Dna Cdna Synthesis Kit, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transcriptor first strand complementary dna cdna synthesis kit/product/Roche
    Average 88 stars, based on 75 article reviews
    Price from $9.99 to $1999.99
    transcriptor first strand complementary dna cdna synthesis kit - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    90
    tiangen biotech co fastquant complementary dna cdna first strand synthesis kit
    Decreased Egr-1 expression in kidneys from Fli-1 +/− NZM2410 mice compared to wild-type controls. Total <t>RNA</t> was prepared from kidneys at the age of 18 weeks ( n = 4 in each group). Total RNA was converted to <t>cDNA</t> with the SuperScript First-Strand
    Fastquant Complementary Dna Cdna First Strand Synthesis Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fastquant complementary dna cdna first strand synthesis kit/product/tiangen biotech co
    Average 90 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    fastquant complementary dna cdna first strand synthesis kit - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    91
    Thermo Fisher first strand complementary dna cdna synthesis
    Decreased Egr-1 expression in kidneys from Fli-1 +/− NZM2410 mice compared to wild-type controls. Total <t>RNA</t> was prepared from kidneys at the age of 18 weeks ( n = 4 in each group). Total RNA was converted to <t>cDNA</t> with the SuperScript First-Strand
    First Strand Complementary Dna Cdna Synthesis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 663 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand complementary dna cdna synthesis/product/Thermo Fisher
    Average 91 stars, based on 663 article reviews
    Price from $9.99 to $1999.99
    first strand complementary dna cdna synthesis - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    Image Search Results


    Dectin-1 silencing. DCs were electroporated with Dectin-1 siRNA or non-silencing siRNA and were cultured for 24 h at 37°C. RNA was isolated from 1 × 10 6 cells and was converted to cDNA. RT-PCR was performed to quantify Dectin-1 downregulation on mRNA level. Dectin-1 mRNA expression level of Dectin-1 silenced DCs was calculated compared to a non-silencing control. Data are represented as mean + SEM of n = 5 independent experiments. A student's t -test was performed and significant differences are marked with an asterisk (*** p

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: First Insights in NK—DC Cross-Talk and the Importance of Soluble Factors During Infection With Aspergillus fumigatus

    doi: 10.3389/fcimb.2018.00288

    Figure Lengend Snippet: Dectin-1 silencing. DCs were electroporated with Dectin-1 siRNA or non-silencing siRNA and were cultured for 24 h at 37°C. RNA was isolated from 1 × 10 6 cells and was converted to cDNA. RT-PCR was performed to quantify Dectin-1 downregulation on mRNA level. Dectin-1 mRNA expression level of Dectin-1 silenced DCs was calculated compared to a non-silencing control. Data are represented as mean + SEM of n = 5 independent experiments. A student's t -test was performed and significant differences are marked with an asterisk (*** p

    Article Snippet: Therefore, RNA was isolated from mock silenced and Dectin-1 silenced DCs by RNeasy Mini kit (Qiagen) before cDNA synthesis (First Strand cDNA Synthesis Kit, K1612, Thermo Fisher Scientific) was performed.

    Techniques: Cell Culture, Isolation, Reverse Transcription Polymerase Chain Reaction, Expressing

    Conditional mutagenesis allows a tetracycline-regulated knockdown of TgPSD1mt in T. gondii . A , scheme for generating the Δ tgpsd1mt/Tg PSD1mt-HA r strain. A tetracycline-regulatable copy of Tg PSD1mt-HA was inserted at the UPRT locus, and the TgPSD1mt gene was deleted using a knock-out plasmid ( pTKO-TgPSD1mt-5 ′ UTR-DHFR-TS-TgPSD1mt-3 ′ UTR ). The primer pairs to screen for 5′- and 3′-recombination are depicted in blue. B , PCR testing of the Δ tgpsd1mt / Tg PSD1mt-HA r mutant. Pyrimethamine-resistant parasite clones were screened by genomic PCR using the 5′- and 3′-crossover-specific primers (5′Scr-F1/R1 and 3′Scr-F1/R1). The parental gDNA was included as a negative control. C , PCR analysis to verify the regulation of Tg PSD1mt mRNA by ATc. Total RNA isolated from the mutant or parental strain was used to generate cDNA and amplify Tg PSD1mt using ORF-specific primers. D , immunostaining of the mutant showing ATc-regulated expression of Tg PSD1mt-HA r . The untreated control and drug-treated (1 and 3 days) parasites were stained using anti-HA and anti- Tg F1B antibodies (24-h infection). E , immunoblot image confirming the proteolytic processing and regulation of Tg PSD1mt-HA r protein in the conditional mutant. ATc treatment was performed for 2 or 4 days in cultures, and fresh host-free parasites were subjected to protein isolation and immunoblot analyses. Note that pre-proenzyme, proenzyme, and α-subunit of Tg PSD1mt-HA exhibit an aberrant migration in SDS-PAGE. A nonspecific band, which was not regulatable, was also observed in both strains. Tg Hsp90 (and nonspecific band) served as the loading control.

    Journal: The Journal of Biological Chemistry

    Article Title: Phosphatidylethanolamine Synthesis in the Parasite Mitochondrion Is Required for Efficient Growth but Dispensable for Survival of Toxoplasma gondii *

    doi: 10.1074/jbc.M113.509406

    Figure Lengend Snippet: Conditional mutagenesis allows a tetracycline-regulated knockdown of TgPSD1mt in T. gondii . A , scheme for generating the Δ tgpsd1mt/Tg PSD1mt-HA r strain. A tetracycline-regulatable copy of Tg PSD1mt-HA was inserted at the UPRT locus, and the TgPSD1mt gene was deleted using a knock-out plasmid ( pTKO-TgPSD1mt-5 ′ UTR-DHFR-TS-TgPSD1mt-3 ′ UTR ). The primer pairs to screen for 5′- and 3′-recombination are depicted in blue. B , PCR testing of the Δ tgpsd1mt / Tg PSD1mt-HA r mutant. Pyrimethamine-resistant parasite clones were screened by genomic PCR using the 5′- and 3′-crossover-specific primers (5′Scr-F1/R1 and 3′Scr-F1/R1). The parental gDNA was included as a negative control. C , PCR analysis to verify the regulation of Tg PSD1mt mRNA by ATc. Total RNA isolated from the mutant or parental strain was used to generate cDNA and amplify Tg PSD1mt using ORF-specific primers. D , immunostaining of the mutant showing ATc-regulated expression of Tg PSD1mt-HA r . The untreated control and drug-treated (1 and 3 days) parasites were stained using anti-HA and anti- Tg F1B antibodies (24-h infection). E , immunoblot image confirming the proteolytic processing and regulation of Tg PSD1mt-HA r protein in the conditional mutant. ATc treatment was performed for 2 or 4 days in cultures, and fresh host-free parasites were subjected to protein isolation and immunoblot analyses. Note that pre-proenzyme, proenzyme, and α-subunit of Tg PSD1mt-HA exhibit an aberrant migration in SDS-PAGE. A nonspecific band, which was not regulatable, was also observed in both strains. Tg Hsp90 (and nonspecific band) served as the loading control.

    Article Snippet: RNA purifications, first strand cDNA syntheses, and DNA isolations were performed using commercial kits from Invitrogen and Analytik Jena.

    Techniques: Mutagenesis, Knock-Out, Plasmid Preparation, Polymerase Chain Reaction, Clone Assay, Negative Control, Isolation, Immunostaining, Expressing, Staining, Infection, Migration, SDS Page

    Expression analysis of REM1 . A, An RNA blot containing 1.5 μg of poly(A) + RNA from different organs of Arabidopsis was hybridized with the cDNA REM1 and a probe corresponding to ribosomal protein L3 as a control for gel loading ( RBP4 ). B, Expression analysis of AtREM1 and AP2 by reverse transcriptase (RT)-PCR in different organs from adult plants. RT-PCR products were blotted onto membranes and hybridized with the corresponding probes. C, RT-PCR analysis of expression of REM1 in apices from young seedlings at different stages of development. C, Cotyledon and hypocotyl from young seedlings; IA, inflorescence apex; IF, immature flower, before anthesis; L, leaf; MF, flower at anthesis; R, root; S, stem; Sq, silique.

    Journal: Plant Physiology

    Article Title: AtREM1, a Member of a New Family of B3 Domain-Containing Genes, Is Preferentially Expressed in Reproductive Meristems 1

    doi: 10.1104/pp.010323

    Figure Lengend Snippet: Expression analysis of REM1 . A, An RNA blot containing 1.5 μg of poly(A) + RNA from different organs of Arabidopsis was hybridized with the cDNA REM1 and a probe corresponding to ribosomal protein L3 as a control for gel loading ( RBP4 ). B, Expression analysis of AtREM1 and AP2 by reverse transcriptase (RT)-PCR in different organs from adult plants. RT-PCR products were blotted onto membranes and hybridized with the corresponding probes. C, RT-PCR analysis of expression of REM1 in apices from young seedlings at different stages of development. C, Cotyledon and hypocotyl from young seedlings; IA, inflorescence apex; IF, immature flower, before anthesis; L, leaf; MF, flower at anthesis; R, root; S, stem; Sq, silique.

    Article Snippet: For RT-PCR expression studies in rem1 homozygous lines, 5 μg of total RNA from inflorescence apices was treated with DNase and was employed in first-strand cDNA synthesis using SuperScript II (Gibco-BRL, Grand Island, NY) and an oligo T17 as primer. cDNAs were 250- and 500-fold diluted in subsequent PCR reactions.

    Techniques: Expressing, Northern blot, Reverse Transcription Polymerase Chain Reaction, IA

    IL-37 + T-cells play a protective role in DSS-induced chronic colitis in mice. ( A ) Relative weight curves of WT mice, DSS-induced WT mice, DSS-induced WT mice treated with PBS, and DSS-induced WT mice injected with IL-37 + T-cells in chronic colitis. The statistical significance between the relative weights of DSS-induced mice injected with IL-37 + T-cells versus DSS-induced mice treated with PBS at the end of the experiment is shown. ( B ) The disease activity index (DAI) was determined at day 63 of the study period, based on body weight loss (0, none; 1, 1–5%; 2, 5–10%; 3, 10–20%; 4, > 20%), stool consistency (0, normal; 2, loose stool; 4, diarrhea), and stool blood (0, negative; 2, fecal occult blood test positive; 4, gross bleeding). ( C ) Representative pictures of the colon from indicated treatment cohorts. ( D ) Macroscopic damage score of the colon in the four groups. ( E ) Representative haematoxylin and eosin (H E) staining of colon sections of mice. ( F ) Histological inflammation score. This score comprises the sum of architecture of the bowel, infiltration of neutrophils and mononuclear cells, gobleT-cell depletion, and epithelial cell erosion. Three sections per animal were evaluated. ( G ) Expressions of IFN-γ, IL-1β, TNF-α, and IL-10 mRNAs within colonic tissues in the four groups. Total RNA with the RNAiso Plusi Kit. cDNAs were synthesized by using the RevertAid First Strand cDNA Synthesis Kit according to the manufacturer’s instructions. qPCR amplification reactions were prepared with the SYBR Green PCR Kit and performed using the CFX96 Real-Time System. Data are means ± SEM ( n = 6). * p

    Journal: International Journal of Molecular Sciences

    Article Title: Anti-Inflammatory Effect of IL-37-Producing T-Cell Population in DSS-Induced Chronic Inflammatory Bowel Disease in Mice

    doi: 10.3390/ijms19123884

    Figure Lengend Snippet: IL-37 + T-cells play a protective role in DSS-induced chronic colitis in mice. ( A ) Relative weight curves of WT mice, DSS-induced WT mice, DSS-induced WT mice treated with PBS, and DSS-induced WT mice injected with IL-37 + T-cells in chronic colitis. The statistical significance between the relative weights of DSS-induced mice injected with IL-37 + T-cells versus DSS-induced mice treated with PBS at the end of the experiment is shown. ( B ) The disease activity index (DAI) was determined at day 63 of the study period, based on body weight loss (0, none; 1, 1–5%; 2, 5–10%; 3, 10–20%; 4, > 20%), stool consistency (0, normal; 2, loose stool; 4, diarrhea), and stool blood (0, negative; 2, fecal occult blood test positive; 4, gross bleeding). ( C ) Representative pictures of the colon from indicated treatment cohorts. ( D ) Macroscopic damage score of the colon in the four groups. ( E ) Representative haematoxylin and eosin (H E) staining of colon sections of mice. ( F ) Histological inflammation score. This score comprises the sum of architecture of the bowel, infiltration of neutrophils and mononuclear cells, gobleT-cell depletion, and epithelial cell erosion. Three sections per animal were evaluated. ( G ) Expressions of IFN-γ, IL-1β, TNF-α, and IL-10 mRNAs within colonic tissues in the four groups. Total RNA with the RNAiso Plusi Kit. cDNAs were synthesized by using the RevertAid First Strand cDNA Synthesis Kit according to the manufacturer’s instructions. qPCR amplification reactions were prepared with the SYBR Green PCR Kit and performed using the CFX96 Real-Time System. Data are means ± SEM ( n = 6). * p

    Article Snippet: The Nanodrop spectrophotometer (Thermo Scientific, Waltham, MA, USA) was used to measure absorbance. cDNAs were synthesized by using the RevertAid First Strand cDNA Synthesis Kit (ThermoFisher, Waltham, MA, USA) according to manufacturer’s instructions.

    Techniques: Mouse Assay, Injection, Activity Assay, Staining, Synthesized, Real-time Polymerase Chain Reaction, Amplification, SYBR Green Assay, Polymerase Chain Reaction

    Decreased Egr-1 expression in kidneys from Fli-1 +/− NZM2410 mice compared to wild-type controls. Total RNA was prepared from kidneys at the age of 18 weeks ( n = 4 in each group). Total RNA was converted to cDNA with the SuperScript First-Strand

    Journal: Clinical and Experimental Immunology

    Article Title: Impact of Fli-1 transcription factor on autoantibody and lupus nephritis in NZM2410 mice

    doi: 10.1111/j.1365-2249.2010.04245.x

    Figure Lengend Snippet: Decreased Egr-1 expression in kidneys from Fli-1 +/− NZM2410 mice compared to wild-type controls. Total RNA was prepared from kidneys at the age of 18 weeks ( n = 4 in each group). Total RNA was converted to cDNA with the SuperScript First-Strand

    Article Snippet: Two µg of RNA was used to synthesize cDNA (SuperScript First-Strand Synthesis System; Invitrogen).

    Techniques: Expressing, Mouse Assay