first strand cdna Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher first strand cdna
    Real-time PCR analysis of bone marrow LSK cells indicates that burn injury significantly increases expression of myeloid-specific genes CSF1R ( A ) and CSF2RB ( B ). Total <t>RNA</t> was extracted from LSKs, which was sorted from the total bone marrow (TBM) of sham and PBD7 mice. <t>cDNA</t> was synthesized from the total RNA. Real-time PCR was performed according to manufacturer’s instructions. Bar graphs represent means ± SE of pooled LSKs isolated from 8 mice per group with three repetitions. *** P
    First Strand Cdna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 84286 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand cdna/product/Thermo Fisher
    Average 99 stars, based on 84286 article reviews
    Price from $9.99 to $1999.99
    first strand cdna - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    94
    GE Healthcare first strand cdna
    (A) DNA construct for Vδ6.3Tg mice. The detailed DNA construction is described in Materials and Methods. A mouse TCRδ6.3 <t>cDNA</t> clone was isolated from the RL6.14 hybridoma (from C57BL/6 DN thymocytes) and inserted into the class I promoter/Ig enhancer expression cassette. (B) Expression of Vδ6.3 transgene at an early stage of fetal thymic T cell development. E15 embryos were obtained from TCR-δ −/ − Vδ6.3Tg crosses. Each littermate was typed (data not shown) and analyzed individually. <t>RNA</t> was extracted from total thymocytes and RT-PCR was performed using Vδ6.3 transgene specific primers (Cδ primer and β-globin primer in A, as forward and reverse primers, respectively). β-actin RT-PCR was performed in parallel as a positive control. A 550-bp band specific for Vδ6.3 transgene expression was detected in the thymus of E15 TCRδ −/ − Vδ6.3Tg embryos but not in control (non-Tg) embryos. (C) Expression of endogenous Vδ−Cδ transcripts in wt adult thymic γδ T cells and wt E15 thymocytes. Wt E15 thymocytes were obtained from timed pregnant females. Adult thymic γδ T cells were obtained by electronic sorting after CD4/CD8 complement depletion. RNA was extracted and RT-PCR was performed using specific primers for the indicated Vδ-Cδ transcripts. β-actin RT-PCR was performed in parallel as a positive control.
    First Strand Cdna, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 1628 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand cdna/product/GE Healthcare
    Average 94 stars, based on 1628 article reviews
    Price from $9.99 to $1999.99
    first strand cdna - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    93
    GE Healthcare first strand cdna synthesis
    (A) DNA construct for Vδ6.3Tg mice. The detailed DNA construction is described in Materials and Methods. A mouse TCRδ6.3 <t>cDNA</t> clone was isolated from the RL6.14 hybridoma (from C57BL/6 DN thymocytes) and inserted into the class I promoter/Ig enhancer expression cassette. (B) Expression of Vδ6.3 transgene at an early stage of fetal thymic T cell development. E15 embryos were obtained from TCR-δ −/ − Vδ6.3Tg crosses. Each littermate was typed (data not shown) and analyzed individually. <t>RNA</t> was extracted from total thymocytes and RT-PCR was performed using Vδ6.3 transgene specific primers (Cδ primer and β-globin primer in A, as forward and reverse primers, respectively). β-actin RT-PCR was performed in parallel as a positive control. A 550-bp band specific for Vδ6.3 transgene expression was detected in the thymus of E15 TCRδ −/ − Vδ6.3Tg embryos but not in control (non-Tg) embryos. (C) Expression of endogenous Vδ−Cδ transcripts in wt adult thymic γδ T cells and wt E15 thymocytes. Wt E15 thymocytes were obtained from timed pregnant females. Adult thymic γδ T cells were obtained by electronic sorting after CD4/CD8 complement depletion. RNA was extracted and RT-PCR was performed using specific primers for the indicated Vδ-Cδ transcripts. β-actin RT-PCR was performed in parallel as a positive control.
    First Strand Cdna Synthesis, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 751 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand cdna synthesis/product/GE Healthcare
    Average 93 stars, based on 751 article reviews
    Price from $9.99 to $1999.99
    first strand cdna synthesis - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    88
    Roche first strand complementary dna cdna synthesis kit
    Flow chart for comprehensive NF1 mutation detection. Point mutations identified by <t>DNA</t> sequencing with specific primers (step 1) represented 68.8% of the NF1 mutations. Frameshift and nonsense mutations were identified in 31.3% and 18.8% of NF1 patients, respectively. In addition, missense and splice-site mutations were confirmed using <t>cDNA</t> sequencing (step 2) and were observed in 12.5% and 6.3% of NF1 patients, respectively. In the case of a negative result using DNA sequencing, an analysis of NF1 complete and large partial deletions was performed using multiplex ligation-dependent probe amplification (MLPA) (step 3) and occurred in 25.0% of NF1 patients. This comprehensive mutation screening procedure enabled us to identify an NF1 mutation in 93.8% of the NF1 patients in our study. NF1 = neurofibromatosis type 1.
    First Strand Complementary Dna Cdna Synthesis Kit, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand complementary dna cdna synthesis kit/product/Roche
    Average 88 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    first strand complementary dna cdna synthesis kit - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    85
    OriGene first strand complementary dna cdna synthesis system
    Flow chart for comprehensive NF1 mutation detection. Point mutations identified by <t>DNA</t> sequencing with specific primers (step 1) represented 68.8% of the NF1 mutations. Frameshift and nonsense mutations were identified in 31.3% and 18.8% of NF1 patients, respectively. In addition, missense and splice-site mutations were confirmed using <t>cDNA</t> sequencing (step 2) and were observed in 12.5% and 6.3% of NF1 patients, respectively. In the case of a negative result using DNA sequencing, an analysis of NF1 complete and large partial deletions was performed using multiplex ligation-dependent probe amplification (MLPA) (step 3) and occurred in 25.0% of NF1 patients. This comprehensive mutation screening procedure enabled us to identify an NF1 mutation in 93.8% of the NF1 patients in our study. NF1 = neurofibromatosis type 1.
    First Strand Complementary Dna Cdna Synthesis System, supplied by OriGene, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand complementary dna cdna synthesis system/product/OriGene
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    first strand complementary dna cdna synthesis system - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    88
    Roche transcriptor first strand complementary dna cdna synthesis kit
    Flow chart for comprehensive NF1 mutation detection. Point mutations identified by <t>DNA</t> sequencing with specific primers (step 1) represented 68.8% of the NF1 mutations. Frameshift and nonsense mutations were identified in 31.3% and 18.8% of NF1 patients, respectively. In addition, missense and splice-site mutations were confirmed using <t>cDNA</t> sequencing (step 2) and were observed in 12.5% and 6.3% of NF1 patients, respectively. In the case of a negative result using DNA sequencing, an analysis of NF1 complete and large partial deletions was performed using multiplex ligation-dependent probe amplification (MLPA) (step 3) and occurred in 25.0% of NF1 patients. This comprehensive mutation screening procedure enabled us to identify an NF1 mutation in 93.8% of the NF1 patients in our study. NF1 = neurofibromatosis type 1.
    Transcriptor First Strand Complementary Dna Cdna Synthesis Kit, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transcriptor first strand complementary dna cdna synthesis kit/product/Roche
    Average 88 stars, based on 75 article reviews
    Price from $9.99 to $1999.99
    transcriptor first strand complementary dna cdna synthesis kit - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    90
    tiangen biotech co fastquant complementary dna cdna first strand synthesis kit
    Flow chart for comprehensive NF1 mutation detection. Point mutations identified by <t>DNA</t> sequencing with specific primers (step 1) represented 68.8% of the NF1 mutations. Frameshift and nonsense mutations were identified in 31.3% and 18.8% of NF1 patients, respectively. In addition, missense and splice-site mutations were confirmed using <t>cDNA</t> sequencing (step 2) and were observed in 12.5% and 6.3% of NF1 patients, respectively. In the case of a negative result using DNA sequencing, an analysis of NF1 complete and large partial deletions was performed using multiplex ligation-dependent probe amplification (MLPA) (step 3) and occurred in 25.0% of NF1 patients. This comprehensive mutation screening procedure enabled us to identify an NF1 mutation in 93.8% of the NF1 patients in our study. NF1 = neurofibromatosis type 1.
    Fastquant Complementary Dna Cdna First Strand Synthesis Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fastquant complementary dna cdna first strand synthesis kit/product/tiangen biotech co
    Average 90 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    fastquant complementary dna cdna first strand synthesis kit - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    85
    Roche complementary dna cdna first strand
    Inhibition of toll-like receptor-9 (TLR9) expression by human cytomegalovirus (HCMV) and Epstein–Barr virus (EBV). THP-1-derived macrophages infected with HCMVor EBV were collected for RNA extraction 24 h postinfection. Complemetary <t>DNA</t> <t>(cDNA)</t>
    Complementary Dna Cdna First Strand, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complementary dna cdna first strand/product/Roche
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    complementary dna cdna first strand - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    90
    Genecopoeia first strand complementary dna cdna
    Inhibition of toll-like receptor-9 (TLR9) expression by human cytomegalovirus (HCMV) and Epstein–Barr virus (EBV). THP-1-derived macrophages infected with HCMVor EBV were collected for RNA extraction 24 h postinfection. Complemetary <t>DNA</t> <t>(cDNA)</t>
    First Strand Complementary Dna Cdna, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand complementary dna cdna/product/Genecopoeia
    Average 90 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    first strand complementary dna cdna - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    93
    TaKaRa first strand complementary dna cdna
    qPCR assay. (A) Gel electrophoresis analysis of the target and reference gene PCR products. Lanes 1 and 6, 500-bp molecular marker; 2, LRIG2; 3, NTC for LRIG2; 4, GAPDH; and 5, NTC for GAPDH. (B) Melting curve analysis of the target and reference gene PCR products. The SYBR Green qPCR reactions of the (a) LRIG2 and (b) GAPDH genes were performed using a normal sample of the complementary <t>DNA</t> <t>(cDNA).</t> Each peak corresponds to a unique PCR product. (c) NTC reactions showed no PCR product. LRIG2, leucine-rich repeats and immunoglobulin-like domains 2; NTC, non-template negative control; qPCR, quantitative polymerase chain reaction; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; −dI/dT, negative first derivative of the melting curves as a function of temperature.
    First Strand Complementary Dna Cdna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 825 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand complementary dna cdna/product/TaKaRa
    Average 93 stars, based on 825 article reviews
    Price from $9.99 to $1999.99
    first strand complementary dna cdna - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    92
    Toyobo first strand complementary dna cdna
    qPCR assay. (A) Gel electrophoresis analysis of the target and reference gene PCR products. Lanes 1 and 6, 500-bp molecular marker; 2, LRIG2; 3, NTC for LRIG2; 4, GAPDH; and 5, NTC for GAPDH. (B) Melting curve analysis of the target and reference gene PCR products. The SYBR Green qPCR reactions of the (a) LRIG2 and (b) GAPDH genes were performed using a normal sample of the complementary <t>DNA</t> <t>(cDNA).</t> Each peak corresponds to a unique PCR product. (c) NTC reactions showed no PCR product. LRIG2, leucine-rich repeats and immunoglobulin-like domains 2; NTC, non-template negative control; qPCR, quantitative polymerase chain reaction; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; −dI/dT, negative first derivative of the melting curves as a function of temperature.
    First Strand Complementary Dna Cdna, supplied by Toyobo, used in various techniques. Bioz Stars score: 92/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand complementary dna cdna/product/Toyobo
    Average 92 stars, based on 59 article reviews
    Price from $9.99 to $1999.99
    first strand complementary dna cdna - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Bio-Rad first strand complementary dna cdna
    qPCR assay. (A) Gel electrophoresis analysis of the target and reference gene PCR products. Lanes 1 and 6, 500-bp molecular marker; 2, LRIG2; 3, NTC for LRIG2; 4, GAPDH; and 5, NTC for GAPDH. (B) Melting curve analysis of the target and reference gene PCR products. The SYBR Green qPCR reactions of the (a) LRIG2 and (b) GAPDH genes were performed using a normal sample of the complementary <t>DNA</t> <t>(cDNA).</t> Each peak corresponds to a unique PCR product. (c) NTC reactions showed no PCR product. LRIG2, leucine-rich repeats and immunoglobulin-like domains 2; NTC, non-template negative control; qPCR, quantitative polymerase chain reaction; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; −dI/dT, negative first derivative of the melting curves as a function of temperature.
    First Strand Complementary Dna Cdna, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 467 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand complementary dna cdna/product/Bio-Rad
    Average 92 stars, based on 467 article reviews
    Price from $9.99 to $1999.99
    first strand complementary dna cdna - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    93
    Agilent technologies first strand complementary dna cdna
    qPCR assay. (A) Gel electrophoresis analysis of the target and reference gene PCR products. Lanes 1 and 6, 500-bp molecular marker; 2, LRIG2; 3, NTC for LRIG2; 4, GAPDH; and 5, NTC for GAPDH. (B) Melting curve analysis of the target and reference gene PCR products. The SYBR Green qPCR reactions of the (a) LRIG2 and (b) GAPDH genes were performed using a normal sample of the complementary <t>DNA</t> <t>(cDNA).</t> Each peak corresponds to a unique PCR product. (c) NTC reactions showed no PCR product. LRIG2, leucine-rich repeats and immunoglobulin-like domains 2; NTC, non-template negative control; qPCR, quantitative polymerase chain reaction; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; −dI/dT, negative first derivative of the melting curves as a function of temperature.
    First Strand Complementary Dna Cdna, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand complementary dna cdna/product/Agilent technologies
    Average 93 stars, based on 42 article reviews
    Price from $9.99 to $1999.99
    first strand complementary dna cdna - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    92
    Stratagene first strand complementary dna cdna
    qPCR assay. (A) Gel electrophoresis analysis of the target and reference gene PCR products. Lanes 1 and 6, 500-bp molecular marker; 2, LRIG2; 3, NTC for LRIG2; 4, GAPDH; and 5, NTC for GAPDH. (B) Melting curve analysis of the target and reference gene PCR products. The SYBR Green qPCR reactions of the (a) LRIG2 and (b) GAPDH genes were performed using a normal sample of the complementary <t>DNA</t> <t>(cDNA).</t> Each peak corresponds to a unique PCR product. (c) NTC reactions showed no PCR product. LRIG2, leucine-rich repeats and immunoglobulin-like domains 2; NTC, non-template negative control; qPCR, quantitative polymerase chain reaction; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; −dI/dT, negative first derivative of the melting curves as a function of temperature.
    First Strand Complementary Dna Cdna, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand complementary dna cdna/product/Stratagene
    Average 92 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    first strand complementary dna cdna - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    iNtRON Biotechnology first strand complementary dna cdna
    qPCR assay. (A) Gel electrophoresis analysis of the target and reference gene PCR products. Lanes 1 and 6, 500-bp molecular marker; 2, LRIG2; 3, NTC for LRIG2; 4, GAPDH; and 5, NTC for GAPDH. (B) Melting curve analysis of the target and reference gene PCR products. The SYBR Green qPCR reactions of the (a) LRIG2 and (b) GAPDH genes were performed using a normal sample of the complementary <t>DNA</t> <t>(cDNA).</t> Each peak corresponds to a unique PCR product. (c) NTC reactions showed no PCR product. LRIG2, leucine-rich repeats and immunoglobulin-like domains 2; NTC, non-template negative control; qPCR, quantitative polymerase chain reaction; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; −dI/dT, negative first derivative of the melting curves as a function of temperature.
    First Strand Complementary Dna Cdna, supplied by iNtRON Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand complementary dna cdna/product/iNtRON Biotechnology
    Average 92 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    first strand complementary dna cdna - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    90
    tiangen biotech co first strand complementary dna cdna
    qPCR assay. (A) Gel electrophoresis analysis of the target and reference gene PCR products. Lanes 1 and 6, 500-bp molecular marker; 2, LRIG2; 3, NTC for LRIG2; 4, GAPDH; and 5, NTC for GAPDH. (B) Melting curve analysis of the target and reference gene PCR products. The SYBR Green qPCR reactions of the (a) LRIG2 and (b) GAPDH genes were performed using a normal sample of the complementary <t>DNA</t> <t>(cDNA).</t> Each peak corresponds to a unique PCR product. (c) NTC reactions showed no PCR product. LRIG2, leucine-rich repeats and immunoglobulin-like domains 2; NTC, non-template negative control; qPCR, quantitative polymerase chain reaction; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; −dI/dT, negative first derivative of the melting curves as a function of temperature.
    First Strand Complementary Dna Cdna, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand complementary dna cdna/product/tiangen biotech co
    Average 90 stars, based on 50 article reviews
    Price from $9.99 to $1999.99
    first strand complementary dna cdna - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    99
    Thermo Fisher first strand complementary dna cdna synthesis kit
    qPCR assay. (A) Gel electrophoresis analysis of the target and reference gene PCR products. Lanes 1 and 6, 500-bp molecular marker; 2, LRIG2; 3, NTC for LRIG2; 4, GAPDH; and 5, NTC for GAPDH. (B) Melting curve analysis of the target and reference gene PCR products. The SYBR Green qPCR reactions of the (a) LRIG2 and (b) GAPDH genes were performed using a normal sample of the complementary <t>DNA</t> <t>(cDNA).</t> Each peak corresponds to a unique PCR product. (c) NTC reactions showed no PCR product. LRIG2, leucine-rich repeats and immunoglobulin-like domains 2; NTC, non-template negative control; qPCR, quantitative polymerase chain reaction; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; −dI/dT, negative first derivative of the melting curves as a function of temperature.
    First Strand Complementary Dna Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand complementary dna cdna synthesis kit/product/Thermo Fisher
    Average 99 stars, based on 89 article reviews
    Price from $9.99 to $1999.99
    first strand complementary dna cdna synthesis kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    92
    Vazyme Biotech Co first strand complementary dna cdna
    qPCR assay. (A) Gel electrophoresis analysis of the target and reference gene PCR products. Lanes 1 and 6, 500-bp molecular marker; 2, LRIG2; 3, NTC for LRIG2; 4, GAPDH; and 5, NTC for GAPDH. (B) Melting curve analysis of the target and reference gene PCR products. The SYBR Green qPCR reactions of the (a) LRIG2 and (b) GAPDH genes were performed using a normal sample of the complementary <t>DNA</t> <t>(cDNA).</t> Each peak corresponds to a unique PCR product. (c) NTC reactions showed no PCR product. LRIG2, leucine-rich repeats and immunoglobulin-like domains 2; NTC, non-template negative control; qPCR, quantitative polymerase chain reaction; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; −dI/dT, negative first derivative of the melting curves as a function of temperature.
    First Strand Complementary Dna Cdna, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand complementary dna cdna/product/Vazyme Biotech Co
    Average 92 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    first strand complementary dna cdna - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    99
    Thermo Fisher cdna
    Decreased Egr-1 expression in kidneys from Fli-1 +/− NZM2410 mice compared to wild-type controls. Total <t>RNA</t> was prepared from kidneys at the age of 18 weeks ( n = 4 in each group). Total RNA was converted to <t>cDNA</t> with the SuperScript First-Strand
    Cdna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 160058 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cdna/product/Thermo Fisher
    Average 99 stars, based on 160058 article reviews
    Price from $9.99 to $1999.99
    cdna - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    Real-time PCR analysis of bone marrow LSK cells indicates that burn injury significantly increases expression of myeloid-specific genes CSF1R ( A ) and CSF2RB ( B ). Total RNA was extracted from LSKs, which was sorted from the total bone marrow (TBM) of sham and PBD7 mice. cDNA was synthesized from the total RNA. Real-time PCR was performed according to manufacturer’s instructions. Bar graphs represent means ± SE of pooled LSKs isolated from 8 mice per group with three repetitions. *** P

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Myelo-erythroid commitment after burn injury is under β-adrenergic control via MafB regulation

    doi: 10.1152/ajpcell.00139.2016

    Figure Lengend Snippet: Real-time PCR analysis of bone marrow LSK cells indicates that burn injury significantly increases expression of myeloid-specific genes CSF1R ( A ) and CSF2RB ( B ). Total RNA was extracted from LSKs, which was sorted from the total bone marrow (TBM) of sham and PBD7 mice. cDNA was synthesized from the total RNA. Real-time PCR was performed according to manufacturer’s instructions. Bar graphs represent means ± SE of pooled LSKs isolated from 8 mice per group with three repetitions. *** P

    Article Snippet: First-strand cDNA was synthesized from 180 ng total RNA using a High-Capacity cDNA Reverse Transcription Kit (Life Technologies, Carlsbad, CA), and reactions were run on a Veriti 96-well Fast Thermocycler (Life Technologies) per the manufacturer’s instructions.

    Techniques: Real-time Polymerase Chain Reaction, Expressing, Mouse Assay, Synthesized, Isolation

    (A) RT-PCR amplification of A. hydrophila flgT internal fragments from cDNA of A. hydrophila AH-3 (1), AH3:: flrA (2), AH-3:: flrBC (3), AH-3:: fliA P (4), AH-3:: lafK (5), and AH-3:: lafS (6) mutants. DNA molecular marker (St). A. hydrophila ribosomal 16S ( rrsA ) amplification was used as a control for cDNA template. RT-PCR amplifications were performed at least twice with total RNA preparations obtained from a minimum of two independent extractions. (B) Promoter sequences of A. hydrophila ATCC7966 T AHA_1089 and AH-3 flgT determined in silico . Italic letters indicates Shine-Dalgarno sequences upstream of AHA_1089 and AH-3 flgT start codon ATG (gray box). The -12 and -24 show sequences for the σ 54 binding.

    Journal: Frontiers in Microbiology

    Article Title: The FlgT Protein Is Involved in Aeromonas hydrophila Polar Flagella Stability and Not Affects Anchorage of Lateral Flagella

    doi: 10.3389/fmicb.2016.01150

    Figure Lengend Snippet: (A) RT-PCR amplification of A. hydrophila flgT internal fragments from cDNA of A. hydrophila AH-3 (1), AH3:: flrA (2), AH-3:: flrBC (3), AH-3:: fliA P (4), AH-3:: lafK (5), and AH-3:: lafS (6) mutants. DNA molecular marker (St). A. hydrophila ribosomal 16S ( rrsA ) amplification was used as a control for cDNA template. RT-PCR amplifications were performed at least twice with total RNA preparations obtained from a minimum of two independent extractions. (B) Promoter sequences of A. hydrophila ATCC7966 T AHA_1089 and AH-3 flgT determined in silico . Italic letters indicates Shine-Dalgarno sequences upstream of AHA_1089 and AH-3 flgT start codon ATG (gray box). The -12 and -24 show sequences for the σ 54 binding.

    Article Snippet: First-strand cDNA synthesis was carried out using the Thermoscript RT-PCR system (Invitrogen) and random primers on 5 μg of total RNA DNase-digested, according to the manufacturer’s instructions.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Marker, In Silico, Binding Assay

    (A) DNA construct for Vδ6.3Tg mice. The detailed DNA construction is described in Materials and Methods. A mouse TCRδ6.3 cDNA clone was isolated from the RL6.14 hybridoma (from C57BL/6 DN thymocytes) and inserted into the class I promoter/Ig enhancer expression cassette. (B) Expression of Vδ6.3 transgene at an early stage of fetal thymic T cell development. E15 embryos were obtained from TCR-δ −/ − Vδ6.3Tg crosses. Each littermate was typed (data not shown) and analyzed individually. RNA was extracted from total thymocytes and RT-PCR was performed using Vδ6.3 transgene specific primers (Cδ primer and β-globin primer in A, as forward and reverse primers, respectively). β-actin RT-PCR was performed in parallel as a positive control. A 550-bp band specific for Vδ6.3 transgene expression was detected in the thymus of E15 TCRδ −/ − Vδ6.3Tg embryos but not in control (non-Tg) embryos. (C) Expression of endogenous Vδ−Cδ transcripts in wt adult thymic γδ T cells and wt E15 thymocytes. Wt E15 thymocytes were obtained from timed pregnant females. Adult thymic γδ T cells were obtained by electronic sorting after CD4/CD8 complement depletion. RNA was extracted and RT-PCR was performed using specific primers for the indicated Vδ-Cδ transcripts. β-actin RT-PCR was performed in parallel as a positive control.

    Journal: The Journal of Experimental Medicine

    Article Title: T Cell Receptor Specificity Is Critical for the Development of Epidermal ?? T Cells

    doi:

    Figure Lengend Snippet: (A) DNA construct for Vδ6.3Tg mice. The detailed DNA construction is described in Materials and Methods. A mouse TCRδ6.3 cDNA clone was isolated from the RL6.14 hybridoma (from C57BL/6 DN thymocytes) and inserted into the class I promoter/Ig enhancer expression cassette. (B) Expression of Vδ6.3 transgene at an early stage of fetal thymic T cell development. E15 embryos were obtained from TCR-δ −/ − Vδ6.3Tg crosses. Each littermate was typed (data not shown) and analyzed individually. RNA was extracted from total thymocytes and RT-PCR was performed using Vδ6.3 transgene specific primers (Cδ primer and β-globin primer in A, as forward and reverse primers, respectively). β-actin RT-PCR was performed in parallel as a positive control. A 550-bp band specific for Vδ6.3 transgene expression was detected in the thymus of E15 TCRδ −/ − Vδ6.3Tg embryos but not in control (non-Tg) embryos. (C) Expression of endogenous Vδ−Cδ transcripts in wt adult thymic γδ T cells and wt E15 thymocytes. Wt E15 thymocytes were obtained from timed pregnant females. Adult thymic γδ T cells were obtained by electronic sorting after CD4/CD8 complement depletion. RNA was extracted and RT-PCR was performed using specific primers for the indicated Vδ-Cδ transcripts. β-actin RT-PCR was performed in parallel as a positive control.

    Article Snippet: The first-strand cDNA from extracted RNA was synthesized with oligo(dT) (Amersham Pharmacia Biotech) in a final volume of 20 μl using AMV reverse transcriptase.

    Techniques: Construct, Mouse Assay, Isolation, Expressing, Reverse Transcription Polymerase Chain Reaction, Positive Control

    RNA extraction, complementary DNA synthesis, reverse transcription–polymerase chain reaction, and sequence analysis.

    Journal: The American Journal of Tropical Medicine and Hygiene

    Article Title: Mosquitoes and Mosquito-Borne Arboviruses in the Qinghai-Tibet Plateau--Focused on the Qinghai Area, China

    doi: 10.4269/ajtmh.2010.09-0649

    Figure Lengend Snippet: RNA extraction, complementary DNA synthesis, reverse transcription–polymerase chain reaction, and sequence analysis.

    Article Snippet: Extraction of viral RNA was done with cell-culture supernatants using the QIAmp Viral RNA kit (Qiagen, Inc., Valencia, CA), and first-strand complementary DNA (cDNA) was synthesized using the Ready-To-Go You Prime First Strand Beads (GE Healthcare, Uppsala, Sweden) according to the manufacturer's procedure.

    Techniques: RNA Extraction, DNA Synthesis, Reverse Transcription Polymerase Chain Reaction, Sequencing

    Flow chart for comprehensive NF1 mutation detection. Point mutations identified by DNA sequencing with specific primers (step 1) represented 68.8% of the NF1 mutations. Frameshift and nonsense mutations were identified in 31.3% and 18.8% of NF1 patients, respectively. In addition, missense and splice-site mutations were confirmed using cDNA sequencing (step 2) and were observed in 12.5% and 6.3% of NF1 patients, respectively. In the case of a negative result using DNA sequencing, an analysis of NF1 complete and large partial deletions was performed using multiplex ligation-dependent probe amplification (MLPA) (step 3) and occurred in 25.0% of NF1 patients. This comprehensive mutation screening procedure enabled us to identify an NF1 mutation in 93.8% of the NF1 patients in our study. NF1 = neurofibromatosis type 1.

    Journal: Medicine

    Article Title: Clinical and Molecular Characterization of NF1 Patients

    doi: 10.1097/MD.0000000000003043

    Figure Lengend Snippet: Flow chart for comprehensive NF1 mutation detection. Point mutations identified by DNA sequencing with specific primers (step 1) represented 68.8% of the NF1 mutations. Frameshift and nonsense mutations were identified in 31.3% and 18.8% of NF1 patients, respectively. In addition, missense and splice-site mutations were confirmed using cDNA sequencing (step 2) and were observed in 12.5% and 6.3% of NF1 patients, respectively. In the case of a negative result using DNA sequencing, an analysis of NF1 complete and large partial deletions was performed using multiplex ligation-dependent probe amplification (MLPA) (step 3) and occurred in 25.0% of NF1 patients. This comprehensive mutation screening procedure enabled us to identify an NF1 mutation in 93.8% of the NF1 patients in our study. NF1 = neurofibromatosis type 1.

    Article Snippet: To prevent illegitimate splicing, blood samples were processed after venipuncture with a maximum delay of 4 h and samples were not stored at 4°C., Reverse transcription was performed using 500 ng of total RNA isolated and random hexamers with a First-Strand complementary DNA (cDNA) Synthesis Kit for RT-PCR (AMV) (Roche Applied Science, Indianapolis, IN).

    Techniques: Flow Cytometry, Mutagenesis, DNA Sequencing, Sequencing, Multiplex Assay, Ligation, Amplification, Multiplex Ligation-dependent Probe Amplification

    Inhibition of toll-like receptor-9 (TLR9) expression by human cytomegalovirus (HCMV) and Epstein–Barr virus (EBV). THP-1-derived macrophages infected with HCMVor EBV were collected for RNA extraction 24 h postinfection. Complemetary DNA (cDNA)

    Journal: Oral microbiology and immunology

    Article Title: Human cytomegalovirus and Epstein–Barr virus inhibit oral bacteria-induced macrophage activation and phagocytosis

    doi: 10.1111/j.1399-302X.2009.00504.x

    Figure Lengend Snippet: Inhibition of toll-like receptor-9 (TLR9) expression by human cytomegalovirus (HCMV) and Epstein–Barr virus (EBV). THP-1-derived macrophages infected with HCMVor EBV were collected for RNA extraction 24 h postinfection. Complemetary DNA (cDNA)

    Article Snippet: Total RNA from virus-infected and non-infected THP-1-derived macrophages were prepared using Trizol reagent (Invitrogen, Carlsbad, CA), and first-strand complementary DNA (cDNA) was synthesized with the transcriptor high fidelity cDNA synthesis kit (Roche, Indianapolis, IN).

    Techniques: Inhibition, Expressing, Derivative Assay, Infection, RNA Extraction

    qPCR assay. (A) Gel electrophoresis analysis of the target and reference gene PCR products. Lanes 1 and 6, 500-bp molecular marker; 2, LRIG2; 3, NTC for LRIG2; 4, GAPDH; and 5, NTC for GAPDH. (B) Melting curve analysis of the target and reference gene PCR products. The SYBR Green qPCR reactions of the (a) LRIG2 and (b) GAPDH genes were performed using a normal sample of the complementary DNA (cDNA). Each peak corresponds to a unique PCR product. (c) NTC reactions showed no PCR product. LRIG2, leucine-rich repeats and immunoglobulin-like domains 2; NTC, non-template negative control; qPCR, quantitative polymerase chain reaction; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; −dI/dT, negative first derivative of the melting curves as a function of temperature.

    Journal: Oncology Letters

    Article Title: LRIG2 expression and prognosis in non-small cell lung cancer

    doi: 10.3892/ol.2014.2157

    Figure Lengend Snippet: qPCR assay. (A) Gel electrophoresis analysis of the target and reference gene PCR products. Lanes 1 and 6, 500-bp molecular marker; 2, LRIG2; 3, NTC for LRIG2; 4, GAPDH; and 5, NTC for GAPDH. (B) Melting curve analysis of the target and reference gene PCR products. The SYBR Green qPCR reactions of the (a) LRIG2 and (b) GAPDH genes were performed using a normal sample of the complementary DNA (cDNA). Each peak corresponds to a unique PCR product. (c) NTC reactions showed no PCR product. LRIG2, leucine-rich repeats and immunoglobulin-like domains 2; NTC, non-template negative control; qPCR, quantitative polymerase chain reaction; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; −dI/dT, negative first derivative of the melting curves as a function of temperature.

    Article Snippet: The first-strand complementary DNA (cDNA) was prepared from total RNA using a first-strand PrimeScript™ RT reagent kit with gDNA Eraser (Takara Bio, Inc., Shiga, Japan).

    Techniques: Real-time Polymerase Chain Reaction, Nucleic Acid Electrophoresis, Polymerase Chain Reaction, Marker, SYBR Green Assay, Negative Control

    Decreased Egr-1 expression in kidneys from Fli-1 +/− NZM2410 mice compared to wild-type controls. Total RNA was prepared from kidneys at the age of 18 weeks ( n = 4 in each group). Total RNA was converted to cDNA with the SuperScript First-Strand

    Journal: Clinical and Experimental Immunology

    Article Title: Impact of Fli-1 transcription factor on autoantibody and lupus nephritis in NZM2410 mice

    doi: 10.1111/j.1365-2249.2010.04245.x

    Figure Lengend Snippet: Decreased Egr-1 expression in kidneys from Fli-1 +/− NZM2410 mice compared to wild-type controls. Total RNA was prepared from kidneys at the age of 18 weeks ( n = 4 in each group). Total RNA was converted to cDNA with the SuperScript First-Strand

    Article Snippet: Two µg of RNA was used to synthesize cDNA (SuperScript First-Strand Synthesis System; Invitrogen).

    Techniques: Expressing, Mouse Assay