fetal bovine serum fbs Thermo Fisher Search Results


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  • 90
    Thermo Fisher fetal bovine serum fbs
    Egr1 directly regulates the Id1 expression in prostate cancer. LNCaP and PC3 cells were first incubated in phenol-red free <t>RPMI-1640</t> medium containing 10% <t>FBS</t> for 48 h. The cells were then treated with indicated concentrations of bombesin for another 72 h before mRNA levels were measured using qRT-PCR. Bombesin induced a dose-dependent upregulation of Egr1 both in LNCaP (A) and PC3 (B) cells. Id1 was also upregulated in the same pattern in LNCaP (C) and PC3 (D) cells. When specific siRNA targeting at Egr1, or the control siRNA were used before 1 nM of bombesin was supplemented, the upregulation of Id1 became withdrawn following the silencing of Egr1 in both cells (E, F, * P
    Fetal Bovine Serum Fbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 71407 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher bovine serum fbs
    PS-ChAP SILAC-based quantitative proteomics analysis during serum deprivation followed by serum replenishing. Light-labeled <t>HeLa</t> cells were serum starved for 72 h and heavy-labeled HeLa cells were starved 72 h then refed with 10% <t>FBS</t> serum for 4 h. (
    Bovine Serum Fbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1534 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    GE Healthcare fetal bovine serum
    PS-ChAP SILAC-based quantitative proteomics analysis during serum deprivation followed by serum replenishing. Light-labeled <t>HeLa</t> cells were serum starved for 72 h and heavy-labeled HeLa cells were starved 72 h then refed with 10% <t>FBS</t> serum for 4 h. (
    Fetal Bovine Serum, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 54250 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher exosome depleted fetal bovine serum
    Oral keratinocytes derived exosomes carry v-miRs and deliver their contents into primary macrophages. (A) Electron microscopy image of exosomes isolated from human oral keratinocytes (HOK). Scale bar, 100 nm. (B) Size of the purified exosomes was analyzed on Nanosight Tracking Analysis. The particle size distribution and calculated original concentration of the HOK-derived exosomes. The arrow indicates the particle size (mode) of 137 nm. (C) Western blot showing detection of exosomal marker CD63 in the exosomal and cell lysates. Exosomes were bound to latex beads and then stained with <t>exosome</t> marker CD81 and labeled with lipid binding dye PKH26. Histograms showing (D) CD81 positive beads and (E) PKH26 staining indicating that isolated exosomes are membrane limiting vesicles. (F) Quantitative RT-PCR showing expression of v-miRs in cellular and exosomal RNA of miR-H1 transfected HOK. (G) High levels of miR-K12-3-3p secreted in the exosomes KSHV-infected BC-3 cells. miR-K12-3-3p expression in KSHV-infected cells and exosomes was analyzed by qPCR. Confocal images showing purified HOK exosomes deliver (H) protein and (I) RNA contents inside primary human Mφ. Scale bar, 20 µm. Student’s t -test was conducted to calculate p -values (* p
    Exosome Depleted Fetal Bovine Serum, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher charcoal stripped fetal bovine serum cs fbs
    Glyceollin I inhibits the LTLT-Ca cell motility. ( a ) 2.5 × 10 4 cells were seeded in the upper chamber of a transwell insert (8 μM pore size) alone (migration) or ( b ) coated with Matrigel ® (invasion). Cells were treated with 10 μM glyceollin I or vehicle control at time of plating. Lower wells contained <t>IMEM</t> supplemented with 10% <t>CS-FBS.</t> After 24 h, migrated or invaded cells were fixed and stained for visualization. Upper panels are representative images of crystal violet stained migratory cells. Results are expressed as the mean unit ± SEM (**** p
    Charcoal Stripped Fetal Bovine Serum Cs Fbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher decomplemented fetal bovine serum
    Glyceollin I inhibits the LTLT-Ca cell motility. ( a ) 2.5 × 10 4 cells were seeded in the upper chamber of a transwell insert (8 μM pore size) alone (migration) or ( b ) coated with Matrigel ® (invasion). Cells were treated with 10 μM glyceollin I or vehicle control at time of plating. Lower wells contained <t>IMEM</t> supplemented with 10% <t>CS-FBS.</t> After 24 h, migrated or invaded cells were fixed and stained for visualization. Upper panels are representative images of crystal violet stained migratory cells. Results are expressed as the mean unit ± SEM (**** p
    Decomplemented Fetal Bovine Serum, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher fetal bovine serum bbf
    Glyceollin I inhibits the LTLT-Ca cell motility. ( a ) 2.5 × 10 4 cells were seeded in the upper chamber of a transwell insert (8 μM pore size) alone (migration) or ( b ) coated with Matrigel ® (invasion). Cells were treated with 10 μM glyceollin I or vehicle control at time of plating. Lower wells contained <t>IMEM</t> supplemented with 10% <t>CS-FBS.</t> After 24 h, migrated or invaded cells were fixed and stained for visualization. Upper panels are representative images of crystal violet stained migratory cells. Results are expressed as the mean unit ± SEM (**** p
    Fetal Bovine Serum Bbf, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 76/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher fetal bovine serum 1640
    Glyceollin I inhibits the LTLT-Ca cell motility. ( a ) 2.5 × 10 4 cells were seeded in the upper chamber of a transwell insert (8 μM pore size) alone (migration) or ( b ) coated with Matrigel ® (invasion). Cells were treated with 10 μM glyceollin I or vehicle control at time of plating. Lower wells contained <t>IMEM</t> supplemented with 10% <t>CS-FBS.</t> After 24 h, migrated or invaded cells were fixed and stained for visualization. Upper panels are representative images of crystal violet stained migratory cells. Results are expressed as the mean unit ± SEM (**** p
    Fetal Bovine Serum 1640, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher gm07492 fetal bovine serum
    Glyceollin I inhibits the LTLT-Ca cell motility. ( a ) 2.5 × 10 4 cells were seeded in the upper chamber of a transwell insert (8 μM pore size) alone (migration) or ( b ) coated with Matrigel ® (invasion). Cells were treated with 10 μM glyceollin I or vehicle control at time of plating. Lower wells contained <t>IMEM</t> supplemented with 10% <t>CS-FBS.</t> After 24 h, migrated or invaded cells were fixed and stained for visualization. Upper panels are representative images of crystal violet stained migratory cells. Results are expressed as the mean unit ± SEM (**** p
    Gm07492 Fetal Bovine Serum, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher fetal bovine serum emem
    Glyceollin I inhibits the LTLT-Ca cell motility. ( a ) 2.5 × 10 4 cells were seeded in the upper chamber of a transwell insert (8 μM pore size) alone (migration) or ( b ) coated with Matrigel ® (invasion). Cells were treated with 10 μM glyceollin I or vehicle control at time of plating. Lower wells contained <t>IMEM</t> supplemented with 10% <t>CS-FBS.</t> After 24 h, migrated or invaded cells were fixed and stained for visualization. Upper panels are representative images of crystal violet stained migratory cells. Results are expressed as the mean unit ± SEM (**** p
    Fetal Bovine Serum Emem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher ultra low igg fetal bovine serum
    Surface expression of CD94/NKG2A, LIR-1 and p49 receptors in lymphoid cells freshly isolated from placenta at term and their role in the HLA-G1 recognition. The lymphoid population analyzed was freshly derived from maternal decidua tissues of placenta at term. ( A ) Cells were stained with the indicated combinations of antibodies of different isotype, followed by appropriate isotype-specific second reagents, and were analyzed by double fluorescence analysis ( Upper ). The TOP-2 mouse antiserum ( Lower ) specifically recognizes the p49 receptor, while a control mouse serum (MO-serum) represents a control serum derived from a nonimmunized mouse ( x axis, green fluorescence; y axis, red fluorescence). In this case, an anti-mouse <t>IgG1</t> (PE-conjugated) has been used as second reagent. Note that, in double fluorescence experiments, both anti-CD16 and anti-CD3 mAbs belonged to the <t>IgG2a</t> subclass. ( B ) Lymphoid populations, freshly isolated from two placentas at term, were analyzed for the ability to lyse 221 cells <t>transfected</t> or not with HLA-G1. The cytolytic activity against 221-G1 was also assessed in the presence of Y9 (anti-CD94) or M401 (anti-LIR-1) mAb, the anti-p49 TOP-2 antiserum, and a MO-serum. A6136 (IgM) mAb in combination with 6A4 F(ab′) 2 , both directed to HLA class I molecules and reacting with HLA-G1, were also used. Note that masking of p49 restored lysis to an extent similar to that obtained by masking HLA-G1. Each histogram represents the mean of triplicate experiments.
    Ultra Low Igg Fetal Bovine Serum, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 187 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher fetal bovine serum fbs mesenchymal stem cell
    Surface expression of CD94/NKG2A, LIR-1 and p49 receptors in lymphoid cells freshly isolated from placenta at term and their role in the HLA-G1 recognition. The lymphoid population analyzed was freshly derived from maternal decidua tissues of placenta at term. ( A ) Cells were stained with the indicated combinations of antibodies of different isotype, followed by appropriate isotype-specific second reagents, and were analyzed by double fluorescence analysis ( Upper ). The TOP-2 mouse antiserum ( Lower ) specifically recognizes the p49 receptor, while a control mouse serum (MO-serum) represents a control serum derived from a nonimmunized mouse ( x axis, green fluorescence; y axis, red fluorescence). In this case, an anti-mouse <t>IgG1</t> (PE-conjugated) has been used as second reagent. Note that, in double fluorescence experiments, both anti-CD16 and anti-CD3 mAbs belonged to the <t>IgG2a</t> subclass. ( B ) Lymphoid populations, freshly isolated from two placentas at term, were analyzed for the ability to lyse 221 cells <t>transfected</t> or not with HLA-G1. The cytolytic activity against 221-G1 was also assessed in the presence of Y9 (anti-CD94) or M401 (anti-LIR-1) mAb, the anti-p49 TOP-2 antiserum, and a MO-serum. A6136 (IgM) mAb in combination with 6A4 F(ab′) 2 , both directed to HLA class I molecules and reacting with HLA-G1, were also used. Note that masking of p49 restored lysis to an extent similar to that obtained by masking HLA-G1. Each histogram represents the mean of triplicate experiments.
    Fetal Bovine Serum Fbs Mesenchymal Stem Cell, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher thj 11t fetal bovine serum
    Sequential evaluation of ROS levels in <t>THJ-16T</t> and <t>THJ-11T</t> cells. The cells were treated with 100 μ M resveratrol for 0 h, 6 h, 12 h, 24 h, and 48 h and stained by DCFH-DA. (a) Flow cytometer determination of intracellular ROS levels in THJ-16T and THJ-11T cells. (b) Demonstration of ROS levels in THJ-16T and THJ-11T cells by fluorescence microscopy. (c) Time-course response of resveratrol-induced mitochondrial superoxide production in THJ-16T cells. All data represent the means ± SD of three independent experiments. ∗ P
    Thj 11t Fetal Bovine Serum, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher kasumi 1 fetal bovine serum
    Parthenolide inhibits protein expressions of DNMT1 in MV4-11 (left) and <t>Kasumi-1</t> (right) cells for 24 h in a dose-dependent manner. MV4-11 and Kasumi-1 cells were incubated with the indicated concentrations of parthenolide (0, 1, 3, and 10 μM). DNMT1 protein levels were detected by Western blot.
    Kasumi 1 Fetal Bovine Serum, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher fetal bovine serum dmem
    Parthenolide inhibits protein expressions of DNMT1 in MV4-11 (left) and <t>Kasumi-1</t> (right) cells for 24 h in a dose-dependent manner. MV4-11 and Kasumi-1 cells were incubated with the indicated concentrations of parthenolide (0, 1, 3, and 10 μM). DNMT1 protein levels were detected by Western blot.
    Fetal Bovine Serum Dmem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher bcp 1 fetal bovine serum
    PCR and Northern blot analyses identify previously unannotated transcripts. (A) Map of KSHV genome nt 21500 to 30000 with letter designations (A to CC) for consecutive 300-bp products. Positive PCR amplifications from a <t>BCP-1</t> TPA-stimulated cell library (dotted line) and a KS tumor cDNA library (dotted-dashed line) are shown and were used as probes for Northern blot analyses. The remaining probes were generated from PCR products using genomic DNA, and primer pairs that did not amplify with either cDNA library. Northern blot analyses were performed using poly(A) RNAs isolated from BC-1 cells that were untreated or treated with TPA as indicated. Previously annotated viral genes with their orientation along with frnk and vnct direct repeat regions are noted. (B) mRNA from BC-1 cells untreated or treated with TPA, PFA, or a combination of TPA and PFA using Probe H to determine directionality and expression pattern. (C) Expression pattern for T 6.1 and T 1.5 transcripts using Probe L with mRNA from BC-1 cells untreated or treated with TPA, PFA, or a combination of TPA and PFA.
    Bcp 1 Fetal Bovine Serum, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Thermo Fisher tet approved fetal bovine serum
    PCR and Northern blot analyses identify previously unannotated transcripts. (A) Map of KSHV genome nt 21500 to 30000 with letter designations (A to CC) for consecutive 300-bp products. Positive PCR amplifications from a <t>BCP-1</t> TPA-stimulated cell library (dotted line) and a KS tumor cDNA library (dotted-dashed line) are shown and were used as probes for Northern blot analyses. The remaining probes were generated from PCR products using genomic DNA, and primer pairs that did not amplify with either cDNA library. Northern blot analyses were performed using poly(A) RNAs isolated from BC-1 cells that were untreated or treated with TPA as indicated. Previously annotated viral genes with their orientation along with frnk and vnct direct repeat regions are noted. (B) mRNA from BC-1 cells untreated or treated with TPA, PFA, or a combination of TPA and PFA using Probe H to determine directionality and expression pattern. (C) Expression pattern for T 6.1 and T 1.5 transcripts using Probe L with mRNA from BC-1 cells untreated or treated with TPA, PFA, or a combination of TPA and PFA.
    Tet Approved Fetal Bovine Serum, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher thp 1 fetal bovine serum fbs
    PCR and Northern blot analyses identify previously unannotated transcripts. (A) Map of KSHV genome nt 21500 to 30000 with letter designations (A to CC) for consecutive 300-bp products. Positive PCR amplifications from a <t>BCP-1</t> TPA-stimulated cell library (dotted line) and a KS tumor cDNA library (dotted-dashed line) are shown and were used as probes for Northern blot analyses. The remaining probes were generated from PCR products using genomic DNA, and primer pairs that did not amplify with either cDNA library. Northern blot analyses were performed using poly(A) RNAs isolated from BC-1 cells that were untreated or treated with TPA as indicated. Previously annotated viral genes with their orientation along with frnk and vnct direct repeat regions are noted. (B) mRNA from BC-1 cells untreated or treated with TPA, PFA, or a combination of TPA and PFA using Probe H to determine directionality and expression pattern. (C) Expression pattern for T 6.1 and T 1.5 transcripts using Probe L with mRNA from BC-1 cells untreated or treated with TPA, PFA, or a combination of TPA and PFA.
    Thp 1 Fetal Bovine Serum Fbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 76/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher caco 2 fetal bovine serum
    Protein intermediates generated during the processing of nsp1a. A monolayer of Yuc8-infected <t>Caco-2</t> cells or mock-infected (M) cells was pulse-labeled with [ 35 S]-Express labeling protein mix for 1 h at 12 hpi. The incorporated label was then chased for the indicated times, and the samples were immunoprecipitated with antibodies to 1a-1, IFLC, 1a-3, or 1a-4, as described in Materials and Methods. Proteins were separated in SDS-15% (A and C) or 11% (B and D) polyacrylamide gels. The migration of the molecular mass markers (in kilodaltons) ( 14 C-methylated proteins; Amersham Pharmacia; CFA626) and of the viral proteins is indicated.
    Caco 2 Fetal Bovine Serum, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher fetal bovine serum fbs 10437 028
    Protein intermediates generated during the processing of nsp1a. A monolayer of Yuc8-infected <t>Caco-2</t> cells or mock-infected (M) cells was pulse-labeled with [ 35 S]-Express labeling protein mix for 1 h at 12 hpi. The incorporated label was then chased for the indicated times, and the samples were immunoprecipitated with antibodies to 1a-1, IFLC, 1a-3, or 1a-4, as described in Materials and Methods. Proteins were separated in SDS-15% (A and C) or 11% (B and D) polyacrylamide gels. The migration of the molecular mass markers (in kilodaltons) ( 14 C-methylated proteins; Amersham Pharmacia; CFA626) and of the viral proteins is indicated.
    Fetal Bovine Serum Fbs 10437 028, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher msc qualified fetal bovine serum
    <t>MSC</t> metabolic activity and proliferation capacity. ( A ) Metabolic activity was measured at 1, 3 and 7 days of MSC culture by resazurin assay. RFU: relative fluorescence units. Statistical differences were evaluated by 2-way ANOVA followed by Turkey’s multiple comparison test ( B ) Representative images of nuclei (Dapi, blue) and Ki-67 immunostaining (pink, white arrows) of control and CIA-derived MSC after 2 days in culture. Scale bar: 50 µm. ( C ). Percentage of Ki-67 + control and CIA-derived MSC, across different experiments. Statistical differences were evaluated by Mann–Whitney test. ( D ) Percentage of Ki-67 + control- derived MSC after 2 days in absence/presence of 10 ng/mL or 100 ng/mL of <t>TNF-α</t> or IL-4. Box plots represent min-to-max distribution of n = 4 to 5 animals per group. * p
    Msc Qualified Fetal Bovine Serum, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 180 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher fetal bovine serum fbs rpmi 1640
    <t>MSC</t> metabolic activity and proliferation capacity. ( A ) Metabolic activity was measured at 1, 3 and 7 days of MSC culture by resazurin assay. RFU: relative fluorescence units. Statistical differences were evaluated by 2-way ANOVA followed by Turkey’s multiple comparison test ( B ) Representative images of nuclei (Dapi, blue) and Ki-67 immunostaining (pink, white arrows) of control and CIA-derived MSC after 2 days in culture. Scale bar: 50 µm. ( C ). Percentage of Ki-67 + control and CIA-derived MSC, across different experiments. Statistical differences were evaluated by Mann–Whitney test. ( D ) Percentage of Ki-67 + control- derived MSC after 2 days in absence/presence of 10 ng/mL or 100 ng/mL of <t>TNF-α</t> or IL-4. Box plots represent min-to-max distribution of n = 4 to 5 animals per group. * p
    Fetal Bovine Serum Fbs Rpmi 1640, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fisher Scientific fetal bovine serum
    <t>MSC</t> metabolic activity and proliferation capacity. ( A ) Metabolic activity was measured at 1, 3 and 7 days of MSC culture by resazurin assay. RFU: relative fluorescence units. Statistical differences were evaluated by 2-way ANOVA followed by Turkey’s multiple comparison test ( B ) Representative images of nuclei (Dapi, blue) and Ki-67 immunostaining (pink, white arrows) of control and CIA-derived MSC after 2 days in culture. Scale bar: 50 µm. ( C ). Percentage of Ki-67 + control and CIA-derived MSC, across different experiments. Statistical differences were evaluated by Mann–Whitney test. ( D ) Percentage of Ki-67 + control- derived MSC after 2 days in absence/presence of 10 ng/mL or 100 ng/mL of <t>TNF-α</t> or IL-4. Box plots represent min-to-max distribution of n = 4 to 5 animals per group. * p
    Fetal Bovine Serum, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 99/100, based on 944 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Atlanta Biologicals fetal bovine serum fbs
    <t>MSC</t> metabolic activity and proliferation capacity. ( A ) Metabolic activity was measured at 1, 3 and 7 days of MSC culture by resazurin assay. RFU: relative fluorescence units. Statistical differences were evaluated by 2-way ANOVA followed by Turkey’s multiple comparison test ( B ) Representative images of nuclei (Dapi, blue) and Ki-67 immunostaining (pink, white arrows) of control and CIA-derived MSC after 2 days in culture. Scale bar: 50 µm. ( C ). Percentage of Ki-67 + control and CIA-derived MSC, across different experiments. Statistical differences were evaluated by Mann–Whitney test. ( D ) Percentage of Ki-67 + control- derived MSC after 2 days in absence/presence of 10 ng/mL or 100 ng/mL of <t>TNF-α</t> or IL-4. Box plots represent min-to-max distribution of n = 4 to 5 animals per group. * p
    Fetal Bovine Serum Fbs, supplied by Atlanta Biologicals, used in various techniques. Bioz Stars score: 99/100, based on 3101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher mesenchymal stem cell qualified fetal bovine serum
    <t>MSC</t> metabolic activity and proliferation capacity. ( A ) Metabolic activity was measured at 1, 3 and 7 days of MSC culture by resazurin assay. RFU: relative fluorescence units. Statistical differences were evaluated by 2-way ANOVA followed by Turkey’s multiple comparison test ( B ) Representative images of nuclei (Dapi, blue) and Ki-67 immunostaining (pink, white arrows) of control and CIA-derived MSC after 2 days in culture. Scale bar: 50 µm. ( C ). Percentage of Ki-67 + control and CIA-derived MSC, across different experiments. Statistical differences were evaluated by Mann–Whitney test. ( D ) Percentage of Ki-67 + control- derived MSC after 2 days in absence/presence of 10 ng/mL or 100 ng/mL of <t>TNF-α</t> or IL-4. Box plots represent min-to-max distribution of n = 4 to 5 animals per group. * p
    Mesenchymal Stem Cell Qualified Fetal Bovine Serum, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher fetal bovine serum plus zeocin
    <t>MSC</t> metabolic activity and proliferation capacity. ( A ) Metabolic activity was measured at 1, 3 and 7 days of MSC culture by resazurin assay. RFU: relative fluorescence units. Statistical differences were evaluated by 2-way ANOVA followed by Turkey’s multiple comparison test ( B ) Representative images of nuclei (Dapi, blue) and Ki-67 immunostaining (pink, white arrows) of control and CIA-derived MSC after 2 days in culture. Scale bar: 50 µm. ( C ). Percentage of Ki-67 + control and CIA-derived MSC, across different experiments. Statistical differences were evaluated by Mann–Whitney test. ( D ) Percentage of Ki-67 + control- derived MSC after 2 days in absence/presence of 10 ng/mL or 100 ng/mL of <t>TNF-α</t> or IL-4. Box plots represent min-to-max distribution of n = 4 to 5 animals per group. * p
    Fetal Bovine Serum Plus Zeocin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher hek293t cells fetal bovine serum
    <t>MSC</t> metabolic activity and proliferation capacity. ( A ) Metabolic activity was measured at 1, 3 and 7 days of MSC culture by resazurin assay. RFU: relative fluorescence units. Statistical differences were evaluated by 2-way ANOVA followed by Turkey’s multiple comparison test ( B ) Representative images of nuclei (Dapi, blue) and Ki-67 immunostaining (pink, white arrows) of control and CIA-derived MSC after 2 days in culture. Scale bar: 50 µm. ( C ). Percentage of Ki-67 + control and CIA-derived MSC, across different experiments. Statistical differences were evaluated by Mann–Whitney test. ( D ) Percentage of Ki-67 + control- derived MSC after 2 days in absence/presence of 10 ng/mL or 100 ng/mL of <t>TNF-α</t> or IL-4. Box plots represent min-to-max distribution of n = 4 to 5 animals per group. * p
    Hek293t Cells Fetal Bovine Serum, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher embryonic stem cell qualified fetal bovine serum
    <t>MSC</t> metabolic activity and proliferation capacity. ( A ) Metabolic activity was measured at 1, 3 and 7 days of MSC culture by resazurin assay. RFU: relative fluorescence units. Statistical differences were evaluated by 2-way ANOVA followed by Turkey’s multiple comparison test ( B ) Representative images of nuclei (Dapi, blue) and Ki-67 immunostaining (pink, white arrows) of control and CIA-derived MSC after 2 days in culture. Scale bar: 50 µm. ( C ). Percentage of Ki-67 + control and CIA-derived MSC, across different experiments. Statistical differences were evaluated by Mann–Whitney test. ( D ) Percentage of Ki-67 + control- derived MSC after 2 days in absence/presence of 10 ng/mL or 100 ng/mL of <t>TNF-α</t> or IL-4. Box plots represent min-to-max distribution of n = 4 to 5 animals per group. * p
    Embryonic Stem Cell Qualified Fetal Bovine Serum, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher s9 activation system fetal bovine serum fbs
    <t>MSC</t> metabolic activity and proliferation capacity. ( A ) Metabolic activity was measured at 1, 3 and 7 days of MSC culture by resazurin assay. RFU: relative fluorescence units. Statistical differences were evaluated by 2-way ANOVA followed by Turkey’s multiple comparison test ( B ) Representative images of nuclei (Dapi, blue) and Ki-67 immunostaining (pink, white arrows) of control and CIA-derived MSC after 2 days in culture. Scale bar: 50 µm. ( C ). Percentage of Ki-67 + control and CIA-derived MSC, across different experiments. Statistical differences were evaluated by Mann–Whitney test. ( D ) Percentage of Ki-67 + control- derived MSC after 2 days in absence/presence of 10 ng/mL or 100 ng/mL of <t>TNF-α</t> or IL-4. Box plots represent min-to-max distribution of n = 4 to 5 animals per group. * p
    S9 Activation System Fetal Bovine Serum Fbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher nonheat inactivated fetal bovine serum fbs
    <t>MSC</t> metabolic activity and proliferation capacity. ( A ) Metabolic activity was measured at 1, 3 and 7 days of MSC culture by resazurin assay. RFU: relative fluorescence units. Statistical differences were evaluated by 2-way ANOVA followed by Turkey’s multiple comparison test ( B ) Representative images of nuclei (Dapi, blue) and Ki-67 immunostaining (pink, white arrows) of control and CIA-derived MSC after 2 days in culture. Scale bar: 50 µm. ( C ). Percentage of Ki-67 + control and CIA-derived MSC, across different experiments. Statistical differences were evaluated by Mann–Whitney test. ( D ) Percentage of Ki-67 + control- derived MSC after 2 days in absence/presence of 10 ng/mL or 100 ng/mL of <t>TNF-α</t> or IL-4. Box plots represent min-to-max distribution of n = 4 to 5 animals per group. * p
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    Thermo Fisher chelex treated fetal bovine serum
    <t>MSC</t> metabolic activity and proliferation capacity. ( A ) Metabolic activity was measured at 1, 3 and 7 days of MSC culture by resazurin assay. RFU: relative fluorescence units. Statistical differences were evaluated by 2-way ANOVA followed by Turkey’s multiple comparison test ( B ) Representative images of nuclei (Dapi, blue) and Ki-67 immunostaining (pink, white arrows) of control and CIA-derived MSC after 2 days in culture. Scale bar: 50 µm. ( C ). Percentage of Ki-67 + control and CIA-derived MSC, across different experiments. Statistical differences were evaluated by Mann–Whitney test. ( D ) Percentage of Ki-67 + control- derived MSC after 2 days in absence/presence of 10 ng/mL or 100 ng/mL of <t>TNF-α</t> or IL-4. Box plots represent min-to-max distribution of n = 4 to 5 animals per group. * p
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    Image Search Results


    Egr1 directly regulates the Id1 expression in prostate cancer. LNCaP and PC3 cells were first incubated in phenol-red free RPMI-1640 medium containing 10% FBS for 48 h. The cells were then treated with indicated concentrations of bombesin for another 72 h before mRNA levels were measured using qRT-PCR. Bombesin induced a dose-dependent upregulation of Egr1 both in LNCaP (A) and PC3 (B) cells. Id1 was also upregulated in the same pattern in LNCaP (C) and PC3 (D) cells. When specific siRNA targeting at Egr1, or the control siRNA were used before 1 nM of bombesin was supplemented, the upregulation of Id1 became withdrawn following the silencing of Egr1 in both cells (E, F, * P

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: Androgen deprivation therapy induces androgen receptor-dependent upregulation of Egr1 in prostate cancers

    doi:

    Figure Lengend Snippet: Egr1 directly regulates the Id1 expression in prostate cancer. LNCaP and PC3 cells were first incubated in phenol-red free RPMI-1640 medium containing 10% FBS for 48 h. The cells were then treated with indicated concentrations of bombesin for another 72 h before mRNA levels were measured using qRT-PCR. Bombesin induced a dose-dependent upregulation of Egr1 both in LNCaP (A) and PC3 (B) cells. Id1 was also upregulated in the same pattern in LNCaP (C) and PC3 (D) cells. When specific siRNA targeting at Egr1, or the control siRNA were used before 1 nM of bombesin was supplemented, the upregulation of Id1 became withdrawn following the silencing of Egr1 in both cells (E, F, * P

    Article Snippet: All cell lines were maintained in RPMI-1640 medium (Gibco, Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco) at 37°C in 5% CO2 .

    Techniques: Expressing, Incubation, Quantitative RT-PCR

    Egr1 was negatively regulated by AR. PC-3 cells were transiently transfected with AR expression plasmid pSGAR2 containing EGFP expression sequence, and 48 h later the cells were detected for EGFP expression using fluorescence microscopy. Most of the transfected cells expressed EGFP, suggesting high AR expression (A); (B) The transfected PC3 cells were collected and analyzed for the AR protein expression using Western blot assay, and a specific AR protein band was detected as compared with the empty plasmid control. PC-3-T: plasmid pSGAR2 transfected PC-3 cells. (C) PC-3 cells were transiently transfected with plasmid pSGAR2 and cultured in phenol-red free RPMI-1640 medium containing 10% CS-FBS for 48 h. Then, the cells were treated with different DHT concentrations for another 48 h before the cell lysates were prepared and measured using qRT-PCR. (D) PC3 cells were transiently transfected in RPMI-1640 medium containing 10% FBS for 48 h. Then, the cells were treated with different flutamide concentrations for another 4 d before mRNA levels were measured using qRT-PCR. All data were normalized to GAPDH RNA and plotted relative to the Egr1 expression levels in PC-3 cells transfected with empty plasmid (* P

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: Androgen deprivation therapy induces androgen receptor-dependent upregulation of Egr1 in prostate cancers

    doi:

    Figure Lengend Snippet: Egr1 was negatively regulated by AR. PC-3 cells were transiently transfected with AR expression plasmid pSGAR2 containing EGFP expression sequence, and 48 h later the cells were detected for EGFP expression using fluorescence microscopy. Most of the transfected cells expressed EGFP, suggesting high AR expression (A); (B) The transfected PC3 cells were collected and analyzed for the AR protein expression using Western blot assay, and a specific AR protein band was detected as compared with the empty plasmid control. PC-3-T: plasmid pSGAR2 transfected PC-3 cells. (C) PC-3 cells were transiently transfected with plasmid pSGAR2 and cultured in phenol-red free RPMI-1640 medium containing 10% CS-FBS for 48 h. Then, the cells were treated with different DHT concentrations for another 48 h before the cell lysates were prepared and measured using qRT-PCR. (D) PC3 cells were transiently transfected in RPMI-1640 medium containing 10% FBS for 48 h. Then, the cells were treated with different flutamide concentrations for another 4 d before mRNA levels were measured using qRT-PCR. All data were normalized to GAPDH RNA and plotted relative to the Egr1 expression levels in PC-3 cells transfected with empty plasmid (* P

    Article Snippet: All cell lines were maintained in RPMI-1640 medium (Gibco, Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco) at 37°C in 5% CO2 .

    Techniques: Transfection, Expressing, Plasmid Preparation, Sequencing, Fluorescence, Microscopy, Western Blot, Cell Culture, Quantitative RT-PCR

    Androgenic regulation of Egr1 in LNCaP or PC-3 cells. LNCaP (A) and PC-3 (C) cells were seeded in six-well culture dishes and incubated in phenol-red free RPMI-1640 medium containing 10% CS-FBS for 48 h. The cells were then treated with indicated concentrations of dihydrotestosterone for another 48 h. LNCaP (B) and PC-3 (D) cells were seeded in six-well culture dishes, incubated in RPMI-1640 medium containing 10% FBS and the indicated concentrations of flutamide for 4 d. After incubation, total RNA of all these cells was isolated and subjected to qRT-PCR analysis. All data have been normalized to GAPDH RNA and plotted relative to the Egr1 expression levels in LNCaP cells incubated in medium without drugs (* P

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: Androgen deprivation therapy induces androgen receptor-dependent upregulation of Egr1 in prostate cancers

    doi:

    Figure Lengend Snippet: Androgenic regulation of Egr1 in LNCaP or PC-3 cells. LNCaP (A) and PC-3 (C) cells were seeded in six-well culture dishes and incubated in phenol-red free RPMI-1640 medium containing 10% CS-FBS for 48 h. The cells were then treated with indicated concentrations of dihydrotestosterone for another 48 h. LNCaP (B) and PC-3 (D) cells were seeded in six-well culture dishes, incubated in RPMI-1640 medium containing 10% FBS and the indicated concentrations of flutamide for 4 d. After incubation, total RNA of all these cells was isolated and subjected to qRT-PCR analysis. All data have been normalized to GAPDH RNA and plotted relative to the Egr1 expression levels in LNCaP cells incubated in medium without drugs (* P

    Article Snippet: All cell lines were maintained in RPMI-1640 medium (Gibco, Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco) at 37°C in 5% CO2 .

    Techniques: Incubation, Isolation, Quantitative RT-PCR, Expressing

    Egr1 was more highly expressed in PC3 cells and was evidently upregulated in LNCaP cells after ADT in vitro . LNCaP and PC3 cells were seeded and cultured in vitro in RPMI-1640 containing 10% FBS for 48 h, and were subsequently collected and detected for Egr1 mRNA (A) and protein (B) levels using qRT-PCR and Western blot. Egr1 was more highly expressed in PC3 than in LNCaP cells (** P

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: Androgen deprivation therapy induces androgen receptor-dependent upregulation of Egr1 in prostate cancers

    doi:

    Figure Lengend Snippet: Egr1 was more highly expressed in PC3 cells and was evidently upregulated in LNCaP cells after ADT in vitro . LNCaP and PC3 cells were seeded and cultured in vitro in RPMI-1640 containing 10% FBS for 48 h, and were subsequently collected and detected for Egr1 mRNA (A) and protein (B) levels using qRT-PCR and Western blot. Egr1 was more highly expressed in PC3 than in LNCaP cells (** P

    Article Snippet: All cell lines were maintained in RPMI-1640 medium (Gibco, Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco) at 37°C in 5% CO2 .

    Techniques: In Vitro, Cell Culture, Quantitative RT-PCR, Western Blot

    (A) Schematic diagram for the time line of CSCs induction. IM: Inducing medium; SM: Sphere culture medium. (B) Tumorsphere formation of different cancer cell lines. (a, b) Panc1 cells were induced with (a) or without (b) the small molecular compounds as described. In the presence of the compounds, cells formed typical tumorspheres (a); in contrast, in the absence of the compounds, only cell patches were observed (b). (c,d,e) Similarly, Bxpc3 (c), H446 (d), and Ecs9706 (e) cells also formed spheres in suspension culture after 4 days induction. Scale bars, 100 μm. (C) Differentiation of induced tumorsphere cells. Induced panc1 sphere cells were cultured in suspension for 5 days. Cells were then digested into single cells and cultured in DMEM containing 10% FBS for 3 days. These cells restored their original morphology (upper panel) as parental panc1 cells (lower panel).

    Journal: Cell Cycle

    Article Title: Rapid induction of pancreatic cancer cells to cancer stem cells via heterochromatin modulation

    doi: 10.1080/15384101.2018.1489180

    Figure Lengend Snippet: (A) Schematic diagram for the time line of CSCs induction. IM: Inducing medium; SM: Sphere culture medium. (B) Tumorsphere formation of different cancer cell lines. (a, b) Panc1 cells were induced with (a) or without (b) the small molecular compounds as described. In the presence of the compounds, cells formed typical tumorspheres (a); in contrast, in the absence of the compounds, only cell patches were observed (b). (c,d,e) Similarly, Bxpc3 (c), H446 (d), and Ecs9706 (e) cells also formed spheres in suspension culture after 4 days induction. Scale bars, 100 μm. (C) Differentiation of induced tumorsphere cells. Induced panc1 sphere cells were cultured in suspension for 5 days. Cells were then digested into single cells and cultured in DMEM containing 10% FBS for 3 days. These cells restored their original morphology (upper panel) as parental panc1 cells (lower panel).

    Article Snippet: Panc1 and H446 cells were cultured in DMEM (Gibco-life Technologies, USA) containing 10% fetal bovine serum (FBS) (Gibco/Invitrogen, Australia), 1% penicillin/streptomycin (PS) (Invitrogen).

    Techniques: Cell Culture

    Induction of heterochromatin alteration. Panc1 cells were cultured in inducing medium (induction) or DMEM containing 10% FBS (control) for 48 hrs. Immunofluorescence images show a significant reduction of heterochromatin markers H3K9me3 and HP1 in induced cells. DNA was counterstained by DAPI. Scale bar is equal to 10 μm.

    Journal: Cell Cycle

    Article Title: Rapid induction of pancreatic cancer cells to cancer stem cells via heterochromatin modulation

    doi: 10.1080/15384101.2018.1489180

    Figure Lengend Snippet: Induction of heterochromatin alteration. Panc1 cells were cultured in inducing medium (induction) or DMEM containing 10% FBS (control) for 48 hrs. Immunofluorescence images show a significant reduction of heterochromatin markers H3K9me3 and HP1 in induced cells. DNA was counterstained by DAPI. Scale bar is equal to 10 μm.

    Article Snippet: Panc1 and H446 cells were cultured in DMEM (Gibco-life Technologies, USA) containing 10% fetal bovine serum (FBS) (Gibco/Invitrogen, Australia), 1% penicillin/streptomycin (PS) (Invitrogen).

    Techniques: Cell Culture, Immunofluorescence

    Synergy of IGF-1R and c-kit-targeting treatments. ( A ) Isobologram plot at IC50 and combination index (CI) of the effects of AG 1094 and AG 1296 on H 209 SCLC cell growth. Tests were conducted in triplicates with 10 4 cells treated with tyrphostins singly or in combination for a total of 72 h in RPMI with 1% FBS, and proliferation of the anchorage-independent cells was assayed by Alamar blue dye reduction (shown is an experiment representative of five). Proliferation CI values were calculated using the classic isobologram equation ( Berenbaum, 1981 ) and are indicated on a graph. CI values

    Journal: British Journal of Cancer

    Article Title: Co-targeting IGF-1R and c-kit: synergistic inhibition of proliferation and induction of apoptosis in H 209 small cell lung cancer cells

    doi: 10.1038/sj.bjc.6601682

    Figure Lengend Snippet: Synergy of IGF-1R and c-kit-targeting treatments. ( A ) Isobologram plot at IC50 and combination index (CI) of the effects of AG 1094 and AG 1296 on H 209 SCLC cell growth. Tests were conducted in triplicates with 10 4 cells treated with tyrphostins singly or in combination for a total of 72 h in RPMI with 1% FBS, and proliferation of the anchorage-independent cells was assayed by Alamar blue dye reduction (shown is an experiment representative of five). Proliferation CI values were calculated using the classic isobologram equation ( Berenbaum, 1981 ) and are indicated on a graph. CI values

    Article Snippet: Cell culture and proliferation assays H 209 SCLC cells were obtained from ATCC (Manassas, VA, USA) and cultured at 37°C with 5% CO2 in RPMI 1640 medium with 10% foetal bovine serum (FBS) (InVitrogen, Gaithersburg, MD, USA) except in growth inhibition assays where the FBS supplement was reduced to 1%.

    Techniques:

    Subcellular localization of the NS5A protein in transiently transfected cells. (A) Schematic representation of the entire NS5A protein and its major structural components. Representation of the amino acid composition of the NS5A fragments used in the subcellular localization studies. Plasmid pHPI 1406 encodes the NS5A protein that lacks the first 162 amino acids from the N terminus. Abbreviations: AD I, acidic domain I; ADII, acidic domain II; PRR, proline-rich region. Asp 154 ). (B) Vero or HuH-7 cells, seeded in 10-mm cover glasses, were transfected with either plasmid pHPI 728 (a, b, c, g, h, and i), pHPI 1406 (e and f), or with the empty plasmid pCI (d) and either left untreated (a, d, e, f, and g) or treated with MG132 (5 μΜ; b and h) or ALLN (10 μΜ; c and i), for the last 12 h. Cells were fixed at 36 h p.t. with 3.7% paraformaldehyde, and immunofluorescence analysis was performed by using the affinity-purified NS5A polyclonal antibody. Samples were examined by laser scanning confocal microscopy.

    Journal: Journal of Virology

    Article Title: Calcium-Dependent Calpain Proteases Are Implicated in Processing of the Hepatitis C Virus NS5A Protein

    doi: 10.1128/JVI.78.21.11865-11878.2004

    Figure Lengend Snippet: Subcellular localization of the NS5A protein in transiently transfected cells. (A) Schematic representation of the entire NS5A protein and its major structural components. Representation of the amino acid composition of the NS5A fragments used in the subcellular localization studies. Plasmid pHPI 1406 encodes the NS5A protein that lacks the first 162 amino acids from the N terminus. Abbreviations: AD I, acidic domain I; ADII, acidic domain II; PRR, proline-rich region. Asp 154 ). (B) Vero or HuH-7 cells, seeded in 10-mm cover glasses, were transfected with either plasmid pHPI 728 (a, b, c, g, h, and i), pHPI 1406 (e and f), or with the empty plasmid pCI (d) and either left untreated (a, d, e, f, and g) or treated with MG132 (5 μΜ; b and h) or ALLN (10 μΜ; c and i), for the last 12 h. Cells were fixed at 36 h p.t. with 3.7% paraformaldehyde, and immunofluorescence analysis was performed by using the affinity-purified NS5A polyclonal antibody. Samples were examined by laser scanning confocal microscopy.

    Article Snippet: Vero, WRL68, and HuH-7 cells were maintained in Dulbecco's modified Eagle medium (Biochrom KG) supplemented with 5 (Vero) or 10% (WRL68 and HuH-7) fetal bovine serum (Gibco BRL), penicillin and streptomycin (at concentrations of 5 IU ml−1 and 50 mg ml−1 , respectively), 2 mM l -glutamine, and nonessential amino acids (only for HuH-7) (1×; Biochrom KG).

    Techniques: Transfection, Plasmid Preparation, Immunofluorescence, Affinity Purification, Confocal Microscopy

    SYK inhibition in ETV6-RUNX1 cell lines enhances the efficacy of conventional chemotherapeutics. ( a ) Western blot analysis for SYK Y525 and its total form in ETV6-RUNX1 -positive (AT-1, AT-2 and REH) and negative ( NALM-6 and RCH-ACV ) cell lines. ( b ) Cell viability measured by MTT test of ETV6-RUNX1 cell lines treated for 72 h with SYK inhibitors. All experiments were performed at least three times, and data are represented as mean ± SEM. ( c ) Reduction of cell viability, determined by MTT test, in AT-1, AT-2 and REH cells after 48 h of treatment with entospletinib and 1 unit of VDA (corresponding to a cocktail of 1 nM VCR, dex and AraC respectively) alone or in combination ( n = at least three for all experiments). Results are presented as means ± SEM. ( d ) Increased cell death determined by Annexin V/PI staining after 48 h of treatment. AT-1, AT-2 and REH cells were treated with 1 unit of VDA and 50 μM of entospletinib (ento) alone or in combination. The percentage of dead cells, defined as the total of Annexin V + /PI − , Annexin V + /PI + and Annexin V − /PI + , was established after normalizing on DMSO-treated cells. Paired t test; * p ≤ 0.05, ** p ≤ 0.01; *** p ≤ 0.001; n = 3 for all experiments. Results are presented as means ± SEM. VDA and ento concentrations used in these experiments were selected on the basis of MTT test results, by choosing the ones most able to reduce cell viability in combination.

    Journal: International Journal of Molecular Sciences

    Article Title: SYK Targeting Represents a Potential Therapeutic Option for Relapsed Resistant Pediatric ETV6-RUNX1 B-Acute Lymphoblastic Leukemia Patients

    doi: 10.3390/ijms20246175

    Figure Lengend Snippet: SYK inhibition in ETV6-RUNX1 cell lines enhances the efficacy of conventional chemotherapeutics. ( a ) Western blot analysis for SYK Y525 and its total form in ETV6-RUNX1 -positive (AT-1, AT-2 and REH) and negative ( NALM-6 and RCH-ACV ) cell lines. ( b ) Cell viability measured by MTT test of ETV6-RUNX1 cell lines treated for 72 h with SYK inhibitors. All experiments were performed at least three times, and data are represented as mean ± SEM. ( c ) Reduction of cell viability, determined by MTT test, in AT-1, AT-2 and REH cells after 48 h of treatment with entospletinib and 1 unit of VDA (corresponding to a cocktail of 1 nM VCR, dex and AraC respectively) alone or in combination ( n = at least three for all experiments). Results are presented as means ± SEM. ( d ) Increased cell death determined by Annexin V/PI staining after 48 h of treatment. AT-1, AT-2 and REH cells were treated with 1 unit of VDA and 50 μM of entospletinib (ento) alone or in combination. The percentage of dead cells, defined as the total of Annexin V + /PI − , Annexin V + /PI + and Annexin V − /PI + , was established after normalizing on DMSO-treated cells. Paired t test; * p ≤ 0.05, ** p ≤ 0.01; *** p ≤ 0.001; n = 3 for all experiments. Results are presented as means ± SEM. VDA and ento concentrations used in these experiments were selected on the basis of MTT test results, by choosing the ones most able to reduce cell viability in combination.

    Article Snippet: Cells were cultured in RPMI 1640 (GIBCO, Thermo Fisher Scientific, Waltham, MA, USA) with 10% (REH) or 20% (AT-1 and AT-2) fetal bovine serum (FBS; GIBCO), glutamine (2 mM/L; GIBCO), penicillin (100 U/mL; GIBCO) and streptomycin (100 mg/mL) (GIBCO), and maintained at 37 °C in a humidified atmosphere with 5% CO2 .

    Techniques: Inhibition, Western Blot, MTT Assay, Staining

    PS-ChAP SILAC-based quantitative proteomics analysis during serum deprivation followed by serum replenishing. Light-labeled HeLa cells were serum starved for 72 h and heavy-labeled HeLa cells were starved 72 h then refed with 10% FBS serum for 4 h. (

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Monitoring Cellular Phosphorylation Signaling Pathways into Chromatin and Down to the Gene Level *

    doi: 10.1074/mcp.M115.053421

    Figure Lengend Snippet: PS-ChAP SILAC-based quantitative proteomics analysis during serum deprivation followed by serum replenishing. Light-labeled HeLa cells were serum starved for 72 h and heavy-labeled HeLa cells were starved 72 h then refed with 10% FBS serum for 4 h. (

    Article Snippet: HeLa cells were cultured in SILAC media supplemented with 10% dialyzed fetal bovine serum (FBS) (Invitrogen) (10 kDa cut-off) and 1X penicillin/streptomycin in a humidified atmosphere containing 5% CO2 .

    Techniques: Labeling

    Oral keratinocytes derived exosomes carry v-miRs and deliver their contents into primary macrophages. (A) Electron microscopy image of exosomes isolated from human oral keratinocytes (HOK). Scale bar, 100 nm. (B) Size of the purified exosomes was analyzed on Nanosight Tracking Analysis. The particle size distribution and calculated original concentration of the HOK-derived exosomes. The arrow indicates the particle size (mode) of 137 nm. (C) Western blot showing detection of exosomal marker CD63 in the exosomal and cell lysates. Exosomes were bound to latex beads and then stained with exosome marker CD81 and labeled with lipid binding dye PKH26. Histograms showing (D) CD81 positive beads and (E) PKH26 staining indicating that isolated exosomes are membrane limiting vesicles. (F) Quantitative RT-PCR showing expression of v-miRs in cellular and exosomal RNA of miR-H1 transfected HOK. (G) High levels of miR-K12-3-3p secreted in the exosomes KSHV-infected BC-3 cells. miR-K12-3-3p expression in KSHV-infected cells and exosomes was analyzed by qPCR. Confocal images showing purified HOK exosomes deliver (H) protein and (I) RNA contents inside primary human Mφ. Scale bar, 20 µm. Student’s t -test was conducted to calculate p -values (* p

    Journal: Frontiers in Immunology

    Article Title: Viral miRNAs Alter Host Cell miRNA Profiles and Modulate Innate Immune Responses

    doi: 10.3389/fimmu.2018.00433

    Figure Lengend Snippet: Oral keratinocytes derived exosomes carry v-miRs and deliver their contents into primary macrophages. (A) Electron microscopy image of exosomes isolated from human oral keratinocytes (HOK). Scale bar, 100 nm. (B) Size of the purified exosomes was analyzed on Nanosight Tracking Analysis. The particle size distribution and calculated original concentration of the HOK-derived exosomes. The arrow indicates the particle size (mode) of 137 nm. (C) Western blot showing detection of exosomal marker CD63 in the exosomal and cell lysates. Exosomes were bound to latex beads and then stained with exosome marker CD81 and labeled with lipid binding dye PKH26. Histograms showing (D) CD81 positive beads and (E) PKH26 staining indicating that isolated exosomes are membrane limiting vesicles. (F) Quantitative RT-PCR showing expression of v-miRs in cellular and exosomal RNA of miR-H1 transfected HOK. (G) High levels of miR-K12-3-3p secreted in the exosomes KSHV-infected BC-3 cells. miR-K12-3-3p expression in KSHV-infected cells and exosomes was analyzed by qPCR. Confocal images showing purified HOK exosomes deliver (H) protein and (I) RNA contents inside primary human Mφ. Scale bar, 20 µm. Student’s t -test was conducted to calculate p -values (* p

    Article Snippet: Cell lines were cultured in RPMI1640 media (Fisher Scientific, Hampton, NH, USA) containing a final concentration of 2 mM l -glutamine, 10% exosome-depleted fetal bovine serum (Thermo Fisher Scientific), penicillin G (100 U/mL), and streptomycin sulfate (100 µg/mL), at 37°C in 5% CO2 .

    Techniques: Derivative Assay, Electron Microscopy, Isolation, Purification, Concentration Assay, Western Blot, Marker, Staining, Labeling, Binding Assay, Quantitative RT-PCR, Expressing, Transfection, Infection, Real-time Polymerase Chain Reaction

    Glyceollin I inhibits the LTLT-Ca cell motility. ( a ) 2.5 × 10 4 cells were seeded in the upper chamber of a transwell insert (8 μM pore size) alone (migration) or ( b ) coated with Matrigel ® (invasion). Cells were treated with 10 μM glyceollin I or vehicle control at time of plating. Lower wells contained IMEM supplemented with 10% CS-FBS. After 24 h, migrated or invaded cells were fixed and stained for visualization. Upper panels are representative images of crystal violet stained migratory cells. Results are expressed as the mean unit ± SEM (**** p

    Journal: International Journal of Environmental Research and Public Health

    Article Title: Glyceollin I Reverses Epithelial to Mesenchymal Transition in Letrozole Resistant Breast Cancer through ZEB1

    doi: 10.3390/ijerph13010010

    Figure Lengend Snippet: Glyceollin I inhibits the LTLT-Ca cell motility. ( a ) 2.5 × 10 4 cells were seeded in the upper chamber of a transwell insert (8 μM pore size) alone (migration) or ( b ) coated with Matrigel ® (invasion). Cells were treated with 10 μM glyceollin I or vehicle control at time of plating. Lower wells contained IMEM supplemented with 10% CS-FBS. After 24 h, migrated or invaded cells were fixed and stained for visualization. Upper panels are representative images of crystal violet stained migratory cells. Results are expressed as the mean unit ± SEM (**** p

    Article Snippet: Human LTLT-Ca cells (long-term letrozole treated MCF-7 cells stably transfected with the human aromatase gene) were generously provided by Dr. Angela Brodie and were cultured in 75-cm2 flasks in phenol red-free IMEM (Invitrogen) supplemented with 10% charcoal-stripped fetal bovine serum (CS-FBS), penicillin-streptomycin, antimycotic-antibiotic (10,000 U/mL penicillin G sodium; 10,000 μg/mL streptomycin sulfate); and 25 μg/mL amphotericin B (Fungizone), 7.5 μg/mL geneticin (Invitrogen) and 1 μM letrozole (Sigma).

    Techniques: Migration, Staining

    Surface expression of CD94/NKG2A, LIR-1 and p49 receptors in lymphoid cells freshly isolated from placenta at term and their role in the HLA-G1 recognition. The lymphoid population analyzed was freshly derived from maternal decidua tissues of placenta at term. ( A ) Cells were stained with the indicated combinations of antibodies of different isotype, followed by appropriate isotype-specific second reagents, and were analyzed by double fluorescence analysis ( Upper ). The TOP-2 mouse antiserum ( Lower ) specifically recognizes the p49 receptor, while a control mouse serum (MO-serum) represents a control serum derived from a nonimmunized mouse ( x axis, green fluorescence; y axis, red fluorescence). In this case, an anti-mouse IgG1 (PE-conjugated) has been used as second reagent. Note that, in double fluorescence experiments, both anti-CD16 and anti-CD3 mAbs belonged to the IgG2a subclass. ( B ) Lymphoid populations, freshly isolated from two placentas at term, were analyzed for the ability to lyse 221 cells transfected or not with HLA-G1. The cytolytic activity against 221-G1 was also assessed in the presence of Y9 (anti-CD94) or M401 (anti-LIR-1) mAb, the anti-p49 TOP-2 antiserum, and a MO-serum. A6136 (IgM) mAb in combination with 6A4 F(ab′) 2 , both directed to HLA class I molecules and reacting with HLA-G1, were also used. Note that masking of p49 restored lysis to an extent similar to that obtained by masking HLA-G1. Each histogram represents the mean of triplicate experiments.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Inhibitory receptors sensing HLA-G1 molecules in pregnancy: Decidua-associated natural killer cells express LIR-1 and CD94/NKG2A and acquire p49, an HLA-G1-specific receptor

    doi:

    Figure Lengend Snippet: Surface expression of CD94/NKG2A, LIR-1 and p49 receptors in lymphoid cells freshly isolated from placenta at term and their role in the HLA-G1 recognition. The lymphoid population analyzed was freshly derived from maternal decidua tissues of placenta at term. ( A ) Cells were stained with the indicated combinations of antibodies of different isotype, followed by appropriate isotype-specific second reagents, and were analyzed by double fluorescence analysis ( Upper ). The TOP-2 mouse antiserum ( Lower ) specifically recognizes the p49 receptor, while a control mouse serum (MO-serum) represents a control serum derived from a nonimmunized mouse ( x axis, green fluorescence; y axis, red fluorescence). In this case, an anti-mouse IgG1 (PE-conjugated) has been used as second reagent. Note that, in double fluorescence experiments, both anti-CD16 and anti-CD3 mAbs belonged to the IgG2a subclass. ( B ) Lymphoid populations, freshly isolated from two placentas at term, were analyzed for the ability to lyse 221 cells transfected or not with HLA-G1. The cytolytic activity against 221-G1 was also assessed in the presence of Y9 (anti-CD94) or M401 (anti-LIR-1) mAb, the anti-p49 TOP-2 antiserum, and a MO-serum. A6136 (IgM) mAb in combination with 6A4 F(ab′) 2 , both directed to HLA class I molecules and reacting with HLA-G1, were also used. Note that masking of p49 restored lysis to an extent similar to that obtained by masking HLA-G1. Each histogram represents the mean of triplicate experiments.

    Article Snippet: COS-7 cells were transfected by the DEAE–dextran method and cultured in DMEM supplemented with 10% Ultra-Low IgG Fetal Bovine Serum (GIBCO/BRL).

    Techniques: Expressing, Isolation, Derivative Assay, Staining, Fluorescence, Transfection, Activity Assay, Lysis

    Specific binding of the anti-p49 TOP-2 antiserum to p49 cell transfectants. ( A ) Binding of p49-Fc soluble molecule to HLA-G1 cell transfectants. p49-Fc has been incubated with HLA-G1 221-transfected cells ( Left ) or with 221 untransfected cells ( Right ) followed by anti-human IgG PE-conjugated mAb. White profiles represent controls stained with the second reagent only. ( B ) Staining of cl.15.212-transfected COS-7 cells by the anti-p49 TOP-2 antiserum. COS-7 cells transfected with cl.15.212-VR1012 construct ( Left) or with an irrelevant cDNA ( Right ) were stained with TOP-2 antiserum followed by a PE-conjugated anti-mouse IgG1. White profiles represent controls stained with the second reagent only.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Inhibitory receptors sensing HLA-G1 molecules in pregnancy: Decidua-associated natural killer cells express LIR-1 and CD94/NKG2A and acquire p49, an HLA-G1-specific receptor

    doi:

    Figure Lengend Snippet: Specific binding of the anti-p49 TOP-2 antiserum to p49 cell transfectants. ( A ) Binding of p49-Fc soluble molecule to HLA-G1 cell transfectants. p49-Fc has been incubated with HLA-G1 221-transfected cells ( Left ) or with 221 untransfected cells ( Right ) followed by anti-human IgG PE-conjugated mAb. White profiles represent controls stained with the second reagent only. ( B ) Staining of cl.15.212-transfected COS-7 cells by the anti-p49 TOP-2 antiserum. COS-7 cells transfected with cl.15.212-VR1012 construct ( Left) or with an irrelevant cDNA ( Right ) were stained with TOP-2 antiserum followed by a PE-conjugated anti-mouse IgG1. White profiles represent controls stained with the second reagent only.

    Article Snippet: COS-7 cells were transfected by the DEAE–dextran method and cultured in DMEM supplemented with 10% Ultra-Low IgG Fetal Bovine Serum (GIBCO/BRL).

    Techniques: Binding Assay, Incubation, Transfection, Staining, Construct

    Sequential evaluation of ROS levels in THJ-16T and THJ-11T cells. The cells were treated with 100 μ M resveratrol for 0 h, 6 h, 12 h, 24 h, and 48 h and stained by DCFH-DA. (a) Flow cytometer determination of intracellular ROS levels in THJ-16T and THJ-11T cells. (b) Demonstration of ROS levels in THJ-16T and THJ-11T cells by fluorescence microscopy. (c) Time-course response of resveratrol-induced mitochondrial superoxide production in THJ-16T cells. All data represent the means ± SD of three independent experiments. ∗ P

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Correlation of Reactive Oxygen Species Levels with Resveratrol Sensitivities of Anaplastic Thyroid Cancer Cells

    doi: 10.1155/2018/6235417

    Figure Lengend Snippet: Sequential evaluation of ROS levels in THJ-16T and THJ-11T cells. The cells were treated with 100 μ M resveratrol for 0 h, 6 h, 12 h, 24 h, and 48 h and stained by DCFH-DA. (a) Flow cytometer determination of intracellular ROS levels in THJ-16T and THJ-11T cells. (b) Demonstration of ROS levels in THJ-16T and THJ-11T cells by fluorescence microscopy. (c) Time-course response of resveratrol-induced mitochondrial superoxide production in THJ-16T cells. All data represent the means ± SD of three independent experiments. ∗ P

    Article Snippet: These cell lines were maintained in RPMI 1640 (GE Healthcare Life Sciences, HyClone Laboratories, Utah, USA) supplemented with 5% (for THJ-16T) or 10% (for THJ-11T) fetal bovine serum (Gibco Life Science, Grand Island, NY, USA), 100 IU/ml penicillin, and 100 μ g/ml streptomycin in a humidified atmosphere of 5% CO2 in air at 37°C.

    Techniques: Staining, Flow Cytometry, Cytometry, Fluorescence, Microscopy

    SOD2 and CAT levels in THJ-16T and THJ-11T cells. Western blot and gray density analyses of SOD2 and CAT in THJ-16T (a) and THJ-11T cells (b). β -Actin as the quantitative control. The statistical significance was set at ∗ P

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Correlation of Reactive Oxygen Species Levels with Resveratrol Sensitivities of Anaplastic Thyroid Cancer Cells

    doi: 10.1155/2018/6235417

    Figure Lengend Snippet: SOD2 and CAT levels in THJ-16T and THJ-11T cells. Western blot and gray density analyses of SOD2 and CAT in THJ-16T (a) and THJ-11T cells (b). β -Actin as the quantitative control. The statistical significance was set at ∗ P

    Article Snippet: These cell lines were maintained in RPMI 1640 (GE Healthcare Life Sciences, HyClone Laboratories, Utah, USA) supplemented with 5% (for THJ-16T) or 10% (for THJ-11T) fetal bovine serum (Gibco Life Science, Grand Island, NY, USA), 100 IU/ml penicillin, and 100 μ g/ml streptomycin in a humidified atmosphere of 5% CO2 in air at 37°C.

    Techniques: Western Blot

    Resveratrol-induced apoptosis in THJ-16T not THJ-11T cells. (a) TUNEL staining was performed on THJ-16T and THJ-11T cells without (N) or with (R) 100 μ M of resveratrol for 48 h. (b) Flow cytometry analysis of Annexin V and PI for apoptosis in THJ-16T and THJ-11T cells without (N) and with (R) resveratrol treatment for 48 h. The resveratrol treatment group had significantly more cell apoptosis than the no resveratrol treatment group had in THJ-16T cells; ∗ P

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Correlation of Reactive Oxygen Species Levels with Resveratrol Sensitivities of Anaplastic Thyroid Cancer Cells

    doi: 10.1155/2018/6235417

    Figure Lengend Snippet: Resveratrol-induced apoptosis in THJ-16T not THJ-11T cells. (a) TUNEL staining was performed on THJ-16T and THJ-11T cells without (N) or with (R) 100 μ M of resveratrol for 48 h. (b) Flow cytometry analysis of Annexin V and PI for apoptosis in THJ-16T and THJ-11T cells without (N) and with (R) resveratrol treatment for 48 h. The resveratrol treatment group had significantly more cell apoptosis than the no resveratrol treatment group had in THJ-16T cells; ∗ P

    Article Snippet: These cell lines were maintained in RPMI 1640 (GE Healthcare Life Sciences, HyClone Laboratories, Utah, USA) supplemented with 5% (for THJ-16T) or 10% (for THJ-11T) fetal bovine serum (Gibco Life Science, Grand Island, NY, USA), 100 IU/ml penicillin, and 100 μ g/ml streptomycin in a humidified atmosphere of 5% CO2 in air at 37°C.

    Techniques: TUNEL Assay, Staining, Flow Cytometry, Cytometry

    Levels of SULT1A1 and 1C2 in ATC cells without and with resveratrol treatment. Western blotting and gray density analyses of SULT1A1 and SULT1C2 expression in THJ-16T and THJ-11T cells without (N) and with (R) 100 μ M resveratrol treatment. β -Actin as the quantitative control. The statistical significance was set at ∗ P

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Correlation of Reactive Oxygen Species Levels with Resveratrol Sensitivities of Anaplastic Thyroid Cancer Cells

    doi: 10.1155/2018/6235417

    Figure Lengend Snippet: Levels of SULT1A1 and 1C2 in ATC cells without and with resveratrol treatment. Western blotting and gray density analyses of SULT1A1 and SULT1C2 expression in THJ-16T and THJ-11T cells without (N) and with (R) 100 μ M resveratrol treatment. β -Actin as the quantitative control. The statistical significance was set at ∗ P

    Article Snippet: These cell lines were maintained in RPMI 1640 (GE Healthcare Life Sciences, HyClone Laboratories, Utah, USA) supplemented with 5% (for THJ-16T) or 10% (for THJ-11T) fetal bovine serum (Gibco Life Science, Grand Island, NY, USA), 100 IU/ml penicillin, and 100 μ g/ml streptomycin in a humidified atmosphere of 5% CO2 in air at 37°C.

    Techniques: Western Blot, Expressing

    Transmission electron microscopic images of THJ-16T and THJ-11T cells without (N) and with (R) 100 μ M resveratrol treatment. Nucleus chromatin condensation and marginalization (red arrow), mitochondrial swelling, and mitochondria cristae breakdown (white arrow) are found in resveratrol-treated THJ-16T cells.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Correlation of Reactive Oxygen Species Levels with Resveratrol Sensitivities of Anaplastic Thyroid Cancer Cells

    doi: 10.1155/2018/6235417

    Figure Lengend Snippet: Transmission electron microscopic images of THJ-16T and THJ-11T cells without (N) and with (R) 100 μ M resveratrol treatment. Nucleus chromatin condensation and marginalization (red arrow), mitochondrial swelling, and mitochondria cristae breakdown (white arrow) are found in resveratrol-treated THJ-16T cells.

    Article Snippet: These cell lines were maintained in RPMI 1640 (GE Healthcare Life Sciences, HyClone Laboratories, Utah, USA) supplemented with 5% (for THJ-16T) or 10% (for THJ-11T) fetal bovine serum (Gibco Life Science, Grand Island, NY, USA), 100 IU/ml penicillin, and 100 μ g/ml streptomycin in a humidified atmosphere of 5% CO2 in air at 37°C.

    Techniques: Transmission Assay

    Distinct response of THJ-16T and THJ-11T cells to resveratrol. (a) MTT assay performed on THJ-16T and THJ-11T cells without (N) and with treatment with 100 μ M resveratrol (R) for 0, 6, 12, 24, and 48 h. Compared with the N group at the same time, ∗ P

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Correlation of Reactive Oxygen Species Levels with Resveratrol Sensitivities of Anaplastic Thyroid Cancer Cells

    doi: 10.1155/2018/6235417

    Figure Lengend Snippet: Distinct response of THJ-16T and THJ-11T cells to resveratrol. (a) MTT assay performed on THJ-16T and THJ-11T cells without (N) and with treatment with 100 μ M resveratrol (R) for 0, 6, 12, 24, and 48 h. Compared with the N group at the same time, ∗ P

    Article Snippet: These cell lines were maintained in RPMI 1640 (GE Healthcare Life Sciences, HyClone Laboratories, Utah, USA) supplemented with 5% (for THJ-16T) or 10% (for THJ-11T) fetal bovine serum (Gibco Life Science, Grand Island, NY, USA), 100 IU/ml penicillin, and 100 μ g/ml streptomycin in a humidified atmosphere of 5% CO2 in air at 37°C.

    Techniques: MTT Assay

    Levels of pro-caspase-9 and pro-caspase-3 and their active forms in THJ-16T and THJ-11T cells. Western blot and gray density analyses of pro-caspase-9 and pro-caspase-3 and their active form expression in THJ-16T and THJ-11T cells without (N) and with (R) 100 μ M resveratrol treatment. β -Actin as the quantitative control. The statistical significance was set at ∗ P

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Correlation of Reactive Oxygen Species Levels with Resveratrol Sensitivities of Anaplastic Thyroid Cancer Cells

    doi: 10.1155/2018/6235417

    Figure Lengend Snippet: Levels of pro-caspase-9 and pro-caspase-3 and their active forms in THJ-16T and THJ-11T cells. Western blot and gray density analyses of pro-caspase-9 and pro-caspase-3 and their active form expression in THJ-16T and THJ-11T cells without (N) and with (R) 100 μ M resveratrol treatment. β -Actin as the quantitative control. The statistical significance was set at ∗ P

    Article Snippet: These cell lines were maintained in RPMI 1640 (GE Healthcare Life Sciences, HyClone Laboratories, Utah, USA) supplemented with 5% (for THJ-16T) or 10% (for THJ-11T) fetal bovine serum (Gibco Life Science, Grand Island, NY, USA), 100 IU/ml penicillin, and 100 μ g/ml streptomycin in a humidified atmosphere of 5% CO2 in air at 37°C.

    Techniques: Western Blot, Expressing

    Parthenolide inhibits protein expressions of DNMT1 in MV4-11 (left) and Kasumi-1 (right) cells for 24 h in a dose-dependent manner. MV4-11 and Kasumi-1 cells were incubated with the indicated concentrations of parthenolide (0, 1, 3, and 10 μM). DNMT1 protein levels were detected by Western blot.

    Journal: The Journal of Pharmacology and Experimental Therapeutics

    Article Title: Modulation of DNA Methylation by a Sesquiterpene Lactone Parthenolide

    doi: 10.1124/jpet.108.147934

    Figure Lengend Snippet: Parthenolide inhibits protein expressions of DNMT1 in MV4-11 (left) and Kasumi-1 (right) cells for 24 h in a dose-dependent manner. MV4-11 and Kasumi-1 cells were incubated with the indicated concentrations of parthenolide (0, 1, 3, and 10 μM). DNMT1 protein levels were detected by Western blot.

    Article Snippet: K562, Kasumi-1, MV4-11 leukemia cell lines were cultured in RPMI 1640 medium (VWR International, Inc., West Chester, PA), and the breast cancer cell line MCF-7 was maintained in Dulbecco's modified Eagle's medium (Mediatech, Herndon, VA) supplemented with 10 or 15% (Kasumi-1) fetal bovine serum (Invitrogen, Carlsbad, CA) and 1% (v/v) penicillin/streptomycin (Invitrogen) antibiotic solution at 37°C in an incubator under 5% CO2 atmosphere.

    Techniques: Incubation, Western Blot

    PCR and Northern blot analyses identify previously unannotated transcripts. (A) Map of KSHV genome nt 21500 to 30000 with letter designations (A to CC) for consecutive 300-bp products. Positive PCR amplifications from a BCP-1 TPA-stimulated cell library (dotted line) and a KS tumor cDNA library (dotted-dashed line) are shown and were used as probes for Northern blot analyses. The remaining probes were generated from PCR products using genomic DNA, and primer pairs that did not amplify with either cDNA library. Northern blot analyses were performed using poly(A) RNAs isolated from BC-1 cells that were untreated or treated with TPA as indicated. Previously annotated viral genes with their orientation along with frnk and vnct direct repeat regions are noted. (B) mRNA from BC-1 cells untreated or treated with TPA, PFA, or a combination of TPA and PFA using Probe H to determine directionality and expression pattern. (C) Expression pattern for T 6.1 and T 1.5 transcripts using Probe L with mRNA from BC-1 cells untreated or treated with TPA, PFA, or a combination of TPA and PFA.

    Journal: Journal of Virology

    Article Title: Transcriptional Analysis of Latent and Inducible Kaposi's Sarcoma-Associated Herpesvirus Transcripts in the K4 to K7 Region †

    doi: 10.1128/JVI.79.24.15099-15106.2005

    Figure Lengend Snippet: PCR and Northern blot analyses identify previously unannotated transcripts. (A) Map of KSHV genome nt 21500 to 30000 with letter designations (A to CC) for consecutive 300-bp products. Positive PCR amplifications from a BCP-1 TPA-stimulated cell library (dotted line) and a KS tumor cDNA library (dotted-dashed line) are shown and were used as probes for Northern blot analyses. The remaining probes were generated from PCR products using genomic DNA, and primer pairs that did not amplify with either cDNA library. Northern blot analyses were performed using poly(A) RNAs isolated from BC-1 cells that were untreated or treated with TPA as indicated. Previously annotated viral genes with their orientation along with frnk and vnct direct repeat regions are noted. (B) mRNA from BC-1 cells untreated or treated with TPA, PFA, or a combination of TPA and PFA using Probe H to determine directionality and expression pattern. (C) Expression pattern for T 6.1 and T 1.5 transcripts using Probe L with mRNA from BC-1 cells untreated or treated with TPA, PFA, or a combination of TPA and PFA.

    Article Snippet: BC-1 (PEL-derived B-cell line coinfected with KSHV and Epstein-Barr virus [EBV]) , BCP-1 (PEL-derived B-cell line infected with KSHV alone) , and BJAB (KSHV- and EBV-negative) cell lines were maintained in RPMI 1640 medium (Cellgro, Herndon, VA) supplemented with 10% (BC-1 and BJAB) or 20% (BCP-1) fetal bovine serum (Gibco-BRL, Grand Island, NY) containing penicillin-streptomycin (100 U/ml), l -glutamine (2 mM), sodium pyruvate (1 mM), HEPES (10 mM), and glucose (4.5 g/liter) at 37°C in the presence of 5% CO2 .

    Techniques: Polymerase Chain Reaction, Northern Blot, cDNA Library Assay, Generated, Isolation, Expressing

    Protein intermediates generated during the processing of nsp1a. A monolayer of Yuc8-infected Caco-2 cells or mock-infected (M) cells was pulse-labeled with [ 35 S]-Express labeling protein mix for 1 h at 12 hpi. The incorporated label was then chased for the indicated times, and the samples were immunoprecipitated with antibodies to 1a-1, IFLC, 1a-3, or 1a-4, as described in Materials and Methods. Proteins were separated in SDS-15% (A and C) or 11% (B and D) polyacrylamide gels. The migration of the molecular mass markers (in kilodaltons) ( 14 C-methylated proteins; Amersham Pharmacia; CFA626) and of the viral proteins is indicated.

    Journal: Journal of Virology

    Article Title: Protein Products of the Open Reading Frames Encoding Nonstructural Proteins of Human Astrovirus Serotype 8

    doi: 10.1128/JVI.77.21.11378-11384.2003

    Figure Lengend Snippet: Protein intermediates generated during the processing of nsp1a. A monolayer of Yuc8-infected Caco-2 cells or mock-infected (M) cells was pulse-labeled with [ 35 S]-Express labeling protein mix for 1 h at 12 hpi. The incorporated label was then chased for the indicated times, and the samples were immunoprecipitated with antibodies to 1a-1, IFLC, 1a-3, or 1a-4, as described in Materials and Methods. Proteins were separated in SDS-15% (A and C) or 11% (B and D) polyacrylamide gels. The migration of the molecular mass markers (in kilodaltons) ( 14 C-methylated proteins; Amersham Pharmacia; CFA626) and of the viral proteins is indicated.

    Article Snippet: Cells were cultured in a CO2 atmosphere at 37°C with minimum essential medium (Eagle's salts) (MEM), supplemented with glutamine, and 10% (BHK-21) or 15% (Caco-2) fetal bovine serum (GIBCO/BRL).

    Techniques: Generated, Infection, Labeling, Immunoprecipitation, Migration, Methylation

    Antisera to recombinant nonstructural Yuc8 proteins recognize specific astrovirus products in infected and transfected cells. (A and B) A monolayer of Caco-2 cells infected with Yuc8 was labeled with [ 35 S]-Express labeling protein mix 12 hpi and harvested 24 h later in TNS buffer. (C and D) BHK-21 cells were infected with the recombinant MVA/T7 virus and transfected with the indicated astrovirus constructs, as described in Materials and Methods. Astrovirus proteins were immunoprecipitated with the indicated sera and separated in SDS-15% (A and C) or 11% (B and D) polyacrylamide gels. Mock-infected cells (M) or cells transfected with the vector (pTM1) were included as controls. In panel D, plasmid pTM1a was included as an additional control for the immunoprecipitation with antibodies to nsp1b. The migration of the molecular mass markers (in kilodaltons) ( 14 C-methylated proteins; Amersham Pharmacia; CFA626) and of the viral proteins is indicated.

    Journal: Journal of Virology

    Article Title: Protein Products of the Open Reading Frames Encoding Nonstructural Proteins of Human Astrovirus Serotype 8

    doi: 10.1128/JVI.77.21.11378-11384.2003

    Figure Lengend Snippet: Antisera to recombinant nonstructural Yuc8 proteins recognize specific astrovirus products in infected and transfected cells. (A and B) A monolayer of Caco-2 cells infected with Yuc8 was labeled with [ 35 S]-Express labeling protein mix 12 hpi and harvested 24 h later in TNS buffer. (C and D) BHK-21 cells were infected with the recombinant MVA/T7 virus and transfected with the indicated astrovirus constructs, as described in Materials and Methods. Astrovirus proteins were immunoprecipitated with the indicated sera and separated in SDS-15% (A and C) or 11% (B and D) polyacrylamide gels. Mock-infected cells (M) or cells transfected with the vector (pTM1) were included as controls. In panel D, plasmid pTM1a was included as an additional control for the immunoprecipitation with antibodies to nsp1b. The migration of the molecular mass markers (in kilodaltons) ( 14 C-methylated proteins; Amersham Pharmacia; CFA626) and of the viral proteins is indicated.

    Article Snippet: Cells were cultured in a CO2 atmosphere at 37°C with minimum essential medium (Eagle's salts) (MEM), supplemented with glutamine, and 10% (BHK-21) or 15% (Caco-2) fetal bovine serum (GIBCO/BRL).

    Techniques: Recombinant, Infection, Transfection, Labeling, Construct, Immunoprecipitation, Plasmid Preparation, Migration, Methylation

    MSC metabolic activity and proliferation capacity. ( A ) Metabolic activity was measured at 1, 3 and 7 days of MSC culture by resazurin assay. RFU: relative fluorescence units. Statistical differences were evaluated by 2-way ANOVA followed by Turkey’s multiple comparison test ( B ) Representative images of nuclei (Dapi, blue) and Ki-67 immunostaining (pink, white arrows) of control and CIA-derived MSC after 2 days in culture. Scale bar: 50 µm. ( C ). Percentage of Ki-67 + control and CIA-derived MSC, across different experiments. Statistical differences were evaluated by Mann–Whitney test. ( D ) Percentage of Ki-67 + control- derived MSC after 2 days in absence/presence of 10 ng/mL or 100 ng/mL of TNF-α or IL-4. Box plots represent min-to-max distribution of n = 4 to 5 animals per group. * p

    Journal: International Journal of Molecular Sciences

    Article Title: The Systemic Immune Response to Collagen-Induced Arthritis and the Impact of Bone Injury in Inflammatory Conditions

    doi: 10.3390/ijms20215436

    Figure Lengend Snippet: MSC metabolic activity and proliferation capacity. ( A ) Metabolic activity was measured at 1, 3 and 7 days of MSC culture by resazurin assay. RFU: relative fluorescence units. Statistical differences were evaluated by 2-way ANOVA followed by Turkey’s multiple comparison test ( B ) Representative images of nuclei (Dapi, blue) and Ki-67 immunostaining (pink, white arrows) of control and CIA-derived MSC after 2 days in culture. Scale bar: 50 µm. ( C ). Percentage of Ki-67 + control and CIA-derived MSC, across different experiments. Statistical differences were evaluated by Mann–Whitney test. ( D ) Percentage of Ki-67 + control- derived MSC after 2 days in absence/presence of 10 ng/mL or 100 ng/mL of TNF-α or IL-4. Box plots represent min-to-max distribution of n = 4 to 5 animals per group. * p

    Article Snippet: After red blood cells lysis, cells were counted and seeded in minimum essential medium alpha modification (α-MEM) supplemented with 10% MSC-qualified fetal bovine serum (ThermoFisher Scientific, Waltham, MA, USA MA, USA) and 1% penicillin G-streptomycin (P/S; Invitrogen, Carlsbad, CA, USA), at 106 /100 mm plate.

    Techniques: Activity Assay, Resazurin Assay, Fluorescence, Immunostaining, Derivative Assay, MANN-WHITNEY

    The culture of MSC-laden HyStem-C constructs under cLIUS stimulation. MSCs were encapsulated at a density of 5 × 10 6 cells/ml of HyStem-C hydrogel and grown in DMEM medium supplemented with 10% FBS, 100 nM dexamethasone, and 50 μg/ml l -ascorbic acid for 6 weeks under cLIUS at 14 kPa (5 MHz, 2.5 Vpp), 20 min/application, and 4 applications/day ( n = 3). Non-cLIUS-stimulated 3D constructs served as controls ( n = 3). Representative images of 4-μm sections of the constructs stained immunohistochemically for collagen II and chondroitin sulfate is shown. Scale bar represents 100 μm

    Journal: Stem Cell Research & Therapy

    Article Title: Preconditioning of mesenchymal stromal cells with low-intensity ultrasound: influence on chondrogenesis and directed SOX9 signaling pathways

    doi: 10.1186/s13287-019-1532-2

    Figure Lengend Snippet: The culture of MSC-laden HyStem-C constructs under cLIUS stimulation. MSCs were encapsulated at a density of 5 × 10 6 cells/ml of HyStem-C hydrogel and grown in DMEM medium supplemented with 10% FBS, 100 nM dexamethasone, and 50 μg/ml l -ascorbic acid for 6 weeks under cLIUS at 14 kPa (5 MHz, 2.5 Vpp), 20 min/application, and 4 applications/day ( n = 3). Non-cLIUS-stimulated 3D constructs served as controls ( n = 3). Representative images of 4-μm sections of the constructs stained immunohistochemically for collagen II and chondroitin sulfate is shown. Scale bar represents 100 μm

    Article Snippet: 2D culture of MSCs Human MSCs were purchased from Lonza (PT-2501, Walkersville, MD, USA) and expanded in alpha-Minimum Essential Medium (α-MEM) supplemented with 10% MSC-qualified fetal bovine serum (FBS) (Gibco, USA), 1× Glutamax (Gibco, USA), and 1× antibiotic-antimycotic solution (Gibco, USA) in a CO2 incubator at 37 °C, 5% carbon dioxide, and 99% humidity.

    Techniques: Construct, Staining