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  • 99
    BioLegend fcγriii
    Fcγriii, supplied by BioLegend, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fcγriii/product/BioLegend
    Average 99 stars, based on 1 article reviews
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    86
    Becton Dickinson fcγriii ii
    Identification of the APL-initiating cells . (A) Kaplan-Meier survival curve of sublethally irradiated mice transplanted with different sorted cell populations isolated from leukemic bone marrow. Injections of sorted cells from subpopulations 3 and 4 (3000 cells) and subpopulation 9 (3000 and 15 000 cells) and 2000 KSL (2000 cells, c-kit + , Sca1 + , lineage − ) isolated from leukemic bone marrow were injected into sublethally irradiated mice. Cells isolated from subpopulations 3 and 4 were able to generate leukemia with a latency of 38 days (○, n = 8). Mice injected with a high concentration of subpopulation 9 (15 000 cells, ■, n = 4) developed leukemia with incomplete penetrance, whereas none of the mice injected with 3000 cells from subpopulation 9 (□, n = 6) or KSL (crosshatched line, n = 6) developed leukemia. (B) Cells accumulating in leukemic bone marrow and their counterparts isolated from wild-type bone marrow were sorted (subpopulations 3 and 4; Bi; red bold rectangle in CD34 + /c-kit + fraction) and stained (Bii) with Wright-Giemsa (original magnification ×100). (Biii) As few as 30 cells accumulating from populations 3 and 4 initiate leukemia in sublethally irradiated recipient mice. Decreasing amounts of cells were injected into sublethally irradiated recipient mice (3000 cells, ○, n = 6; 300 cells, ●, n = 8: and 30 cells, ▲, n = 10). (Biv) Injection of cells accumulating in leukemic bone marrow (subpopulations 3 and 4) results in a 100-fold enrichment of LICs compared with injection of unsorted cells (○, injection of 3000 sorted cells, n = 6, compare with ●, injection of 300 000 unsorted leukemic bone marrow cells, n = 3). (C) Kaplan-Meier survival curve of mice after secondary transplantation. Thirty sorted LICs from subpopulations 3 and 4 (CD34 + , c-kit + , <t>FcγRIII/II</t> + , Gr1 int ) were used in the primary transplantation. Subsequently, 2.5 × 10 5 to 5 × 10 6 unfractionated leukemic spleen cells were isolated from a moribund primary leukemic mouse and transplanted into sublethally irradiated secondary recipients (n = 6), which subsequently developed a secondary leukemia.
    Fcγriii Ii, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fcγriii ii/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fcγriii ii - by Bioz Stars, 2021-06
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    86
    Becton Dickinson fcγriii
    Identification of the APL-initiating cells . (A) Kaplan-Meier survival curve of sublethally irradiated mice transplanted with different sorted cell populations isolated from leukemic bone marrow. Injections of sorted cells from subpopulations 3 and 4 (3000 cells) and subpopulation 9 (3000 and 15 000 cells) and 2000 KSL (2000 cells, c-kit + , Sca1 + , lineage − ) isolated from leukemic bone marrow were injected into sublethally irradiated mice. Cells isolated from subpopulations 3 and 4 were able to generate leukemia with a latency of 38 days (○, n = 8). Mice injected with a high concentration of subpopulation 9 (15 000 cells, ■, n = 4) developed leukemia with incomplete penetrance, whereas none of the mice injected with 3000 cells from subpopulation 9 (□, n = 6) or KSL (crosshatched line, n = 6) developed leukemia. (B) Cells accumulating in leukemic bone marrow and their counterparts isolated from wild-type bone marrow were sorted (subpopulations 3 and 4; Bi; red bold rectangle in CD34 + /c-kit + fraction) and stained (Bii) with Wright-Giemsa (original magnification ×100). (Biii) As few as 30 cells accumulating from populations 3 and 4 initiate leukemia in sublethally irradiated recipient mice. Decreasing amounts of cells were injected into sublethally irradiated recipient mice (3000 cells, ○, n = 6; 300 cells, ●, n = 8: and 30 cells, ▲, n = 10). (Biv) Injection of cells accumulating in leukemic bone marrow (subpopulations 3 and 4) results in a 100-fold enrichment of LICs compared with injection of unsorted cells (○, injection of 3000 sorted cells, n = 6, compare with ●, injection of 300 000 unsorted leukemic bone marrow cells, n = 3). (C) Kaplan-Meier survival curve of mice after secondary transplantation. Thirty sorted LICs from subpopulations 3 and 4 (CD34 + , c-kit + , <t>FcγRIII/II</t> + , Gr1 int ) were used in the primary transplantation. Subsequently, 2.5 × 10 5 to 5 × 10 6 unfractionated leukemic spleen cells were isolated from a moribund primary leukemic mouse and transplanted into sublethally irradiated secondary recipients (n = 6), which subsequently developed a secondary leukemia.
    Fcγriii, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fcγriii/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fcγriii - by Bioz Stars, 2021-06
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    94
    R&D Systems fcγriii
    Identification of the APL-initiating cells . (A) Kaplan-Meier survival curve of sublethally irradiated mice transplanted with different sorted cell populations isolated from leukemic bone marrow. Injections of sorted cells from subpopulations 3 and 4 (3000 cells) and subpopulation 9 (3000 and 15 000 cells) and 2000 KSL (2000 cells, c-kit + , Sca1 + , lineage − ) isolated from leukemic bone marrow were injected into sublethally irradiated mice. Cells isolated from subpopulations 3 and 4 were able to generate leukemia with a latency of 38 days (○, n = 8). Mice injected with a high concentration of subpopulation 9 (15 000 cells, ■, n = 4) developed leukemia with incomplete penetrance, whereas none of the mice injected with 3000 cells from subpopulation 9 (□, n = 6) or KSL (crosshatched line, n = 6) developed leukemia. (B) Cells accumulating in leukemic bone marrow and their counterparts isolated from wild-type bone marrow were sorted (subpopulations 3 and 4; Bi; red bold rectangle in CD34 + /c-kit + fraction) and stained (Bii) with Wright-Giemsa (original magnification ×100). (Biii) As few as 30 cells accumulating from populations 3 and 4 initiate leukemia in sublethally irradiated recipient mice. Decreasing amounts of cells were injected into sublethally irradiated recipient mice (3000 cells, ○, n = 6; 300 cells, ●, n = 8: and 30 cells, ▲, n = 10). (Biv) Injection of cells accumulating in leukemic bone marrow (subpopulations 3 and 4) results in a 100-fold enrichment of LICs compared with injection of unsorted cells (○, injection of 3000 sorted cells, n = 6, compare with ●, injection of 300 000 unsorted leukemic bone marrow cells, n = 3). (C) Kaplan-Meier survival curve of mice after secondary transplantation. Thirty sorted LICs from subpopulations 3 and 4 (CD34 + , c-kit + , <t>FcγRIII/II</t> + , Gr1 int ) were used in the primary transplantation. Subsequently, 2.5 × 10 5 to 5 × 10 6 unfractionated leukemic spleen cells were isolated from a moribund primary leukemic mouse and transplanted into sublethally irradiated secondary recipients (n = 6), which subsequently developed a secondary leukemia.
    Fcγriii, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fcγriii/product/R&D Systems
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fcγriii - by Bioz Stars, 2021-06
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    Image Search Results


    Identification of the APL-initiating cells . (A) Kaplan-Meier survival curve of sublethally irradiated mice transplanted with different sorted cell populations isolated from leukemic bone marrow. Injections of sorted cells from subpopulations 3 and 4 (3000 cells) and subpopulation 9 (3000 and 15 000 cells) and 2000 KSL (2000 cells, c-kit + , Sca1 + , lineage − ) isolated from leukemic bone marrow were injected into sublethally irradiated mice. Cells isolated from subpopulations 3 and 4 were able to generate leukemia with a latency of 38 days (○, n = 8). Mice injected with a high concentration of subpopulation 9 (15 000 cells, ■, n = 4) developed leukemia with incomplete penetrance, whereas none of the mice injected with 3000 cells from subpopulation 9 (□, n = 6) or KSL (crosshatched line, n = 6) developed leukemia. (B) Cells accumulating in leukemic bone marrow and their counterparts isolated from wild-type bone marrow were sorted (subpopulations 3 and 4; Bi; red bold rectangle in CD34 + /c-kit + fraction) and stained (Bii) with Wright-Giemsa (original magnification ×100). (Biii) As few as 30 cells accumulating from populations 3 and 4 initiate leukemia in sublethally irradiated recipient mice. Decreasing amounts of cells were injected into sublethally irradiated recipient mice (3000 cells, ○, n = 6; 300 cells, ●, n = 8: and 30 cells, ▲, n = 10). (Biv) Injection of cells accumulating in leukemic bone marrow (subpopulations 3 and 4) results in a 100-fold enrichment of LICs compared with injection of unsorted cells (○, injection of 3000 sorted cells, n = 6, compare with ●, injection of 300 000 unsorted leukemic bone marrow cells, n = 3). (C) Kaplan-Meier survival curve of mice after secondary transplantation. Thirty sorted LICs from subpopulations 3 and 4 (CD34 + , c-kit + , FcγRIII/II + , Gr1 int ) were used in the primary transplantation. Subsequently, 2.5 × 10 5 to 5 × 10 6 unfractionated leukemic spleen cells were isolated from a moribund primary leukemic mouse and transplanted into sublethally irradiated secondary recipients (n = 6), which subsequently developed a secondary leukemia.

    Journal: Blood

    Article Title: Identification of a myeloid committed progenitor as the cancer-initiating cell in acute promyelocytic leukemia

    doi: 10.1182/blood-2008-10-182071

    Figure Lengend Snippet: Identification of the APL-initiating cells . (A) Kaplan-Meier survival curve of sublethally irradiated mice transplanted with different sorted cell populations isolated from leukemic bone marrow. Injections of sorted cells from subpopulations 3 and 4 (3000 cells) and subpopulation 9 (3000 and 15 000 cells) and 2000 KSL (2000 cells, c-kit + , Sca1 + , lineage − ) isolated from leukemic bone marrow were injected into sublethally irradiated mice. Cells isolated from subpopulations 3 and 4 were able to generate leukemia with a latency of 38 days (○, n = 8). Mice injected with a high concentration of subpopulation 9 (15 000 cells, ■, n = 4) developed leukemia with incomplete penetrance, whereas none of the mice injected with 3000 cells from subpopulation 9 (□, n = 6) or KSL (crosshatched line, n = 6) developed leukemia. (B) Cells accumulating in leukemic bone marrow and their counterparts isolated from wild-type bone marrow were sorted (subpopulations 3 and 4; Bi; red bold rectangle in CD34 + /c-kit + fraction) and stained (Bii) with Wright-Giemsa (original magnification ×100). (Biii) As few as 30 cells accumulating from populations 3 and 4 initiate leukemia in sublethally irradiated recipient mice. Decreasing amounts of cells were injected into sublethally irradiated recipient mice (3000 cells, ○, n = 6; 300 cells, ●, n = 8: and 30 cells, ▲, n = 10). (Biv) Injection of cells accumulating in leukemic bone marrow (subpopulations 3 and 4) results in a 100-fold enrichment of LICs compared with injection of unsorted cells (○, injection of 3000 sorted cells, n = 6, compare with ●, injection of 300 000 unsorted leukemic bone marrow cells, n = 3). (C) Kaplan-Meier survival curve of mice after secondary transplantation. Thirty sorted LICs from subpopulations 3 and 4 (CD34 + , c-kit + , FcγRIII/II + , Gr1 int ) were used in the primary transplantation. Subsequently, 2.5 × 10 5 to 5 × 10 6 unfractionated leukemic spleen cells were isolated from a moribund primary leukemic mouse and transplanted into sublethally irradiated secondary recipients (n = 6), which subsequently developed a secondary leukemia.

    Article Snippet: Then the cells were separated using antibodies against c-kit conjugated with allophycocyanin (clone 2B8; eBioscience) and CD34 conjugated with fluorescein isothiocyanate (RAM34; eBioscience) antigens and analyzed for expression of FCγRIII/II (553143; BD Biosciences PharMingen) and Gr1 (RB6-8C5; eBioscience) antigens.

    Techniques: Irradiation, Mouse Assay, Isolation, Injection, Concentration Assay, Staining, Transplantation Assay

    Definition of the myeloid subsets in bone marrow of wild-type FVB/N mice . (Ai) Cells expressing Sca1, CD45/B220, CD19, CD8, and CD4 were depleted with antibodies. Expression of CD34 and c-kit defined 2 populations (Aii). Each of these 2 populations (CD34 + /c-kit + and CD34 − /c-kit − ) was sorted according to expression of Gr1 and FcγRIII/II and divided into 4 subpopulations (1-4; Aiii) for cells isolated from the CD34 + /c-kit + fraction and 5 subpopulations (5-9) for cells isolated from the CD34 − /c-kit − fraction (Aiv). (B) Sorted cells from CD34 + /ckit + exhibit the morphology of myeloblasts to promyelocytes (subpopulation 1-4), whereas sorted cells from CD34 − /c-kit − exhibit the morphology of increasingly mature myeloid cells from metamyelocytes to mature “band cells.” Sorted cells were subjected to Wright-Giemsa staining, and pictures were acquired at an original magnification ×100 with oil. Numbers in each panel represent the 9 fractions shown in Aiii and iv. (C) Expression of cathepsin G (a primary granule gene), lactoferrin (a secondary granule gene), and MMP9 (a tertiary granule gene) in CD34 + /ckit + and CD34 − /c-kit − sorted cells demonstrate a pattern of granule gene expression correlating with their morphology. Gene expression was assessed by quantitative real-time PCR and normalized to GAPDH levels. Numbers on the x-axis represent the 9 fractions shown in panels Aiii and iv.

    Journal: Blood

    Article Title: Identification of a myeloid committed progenitor as the cancer-initiating cell in acute promyelocytic leukemia

    doi: 10.1182/blood-2008-10-182071

    Figure Lengend Snippet: Definition of the myeloid subsets in bone marrow of wild-type FVB/N mice . (Ai) Cells expressing Sca1, CD45/B220, CD19, CD8, and CD4 were depleted with antibodies. Expression of CD34 and c-kit defined 2 populations (Aii). Each of these 2 populations (CD34 + /c-kit + and CD34 − /c-kit − ) was sorted according to expression of Gr1 and FcγRIII/II and divided into 4 subpopulations (1-4; Aiii) for cells isolated from the CD34 + /c-kit + fraction and 5 subpopulations (5-9) for cells isolated from the CD34 − /c-kit − fraction (Aiv). (B) Sorted cells from CD34 + /ckit + exhibit the morphology of myeloblasts to promyelocytes (subpopulation 1-4), whereas sorted cells from CD34 − /c-kit − exhibit the morphology of increasingly mature myeloid cells from metamyelocytes to mature “band cells.” Sorted cells were subjected to Wright-Giemsa staining, and pictures were acquired at an original magnification ×100 with oil. Numbers in each panel represent the 9 fractions shown in Aiii and iv. (C) Expression of cathepsin G (a primary granule gene), lactoferrin (a secondary granule gene), and MMP9 (a tertiary granule gene) in CD34 + /ckit + and CD34 − /c-kit − sorted cells demonstrate a pattern of granule gene expression correlating with their morphology. Gene expression was assessed by quantitative real-time PCR and normalized to GAPDH levels. Numbers on the x-axis represent the 9 fractions shown in panels Aiii and iv.

    Article Snippet: Then the cells were separated using antibodies against c-kit conjugated with allophycocyanin (clone 2B8; eBioscience) and CD34 conjugated with fluorescein isothiocyanate (RAM34; eBioscience) antigens and analyzed for expression of FCγRIII/II (553143; BD Biosciences PharMingen) and Gr1 (RB6-8C5; eBioscience) antigens.

    Techniques: Mouse Assay, Expressing, Isolation, Staining, Real-time Polymerase Chain Reaction