Journal: Veterinary Sciences

Article Title: Activating Fc Gamma Receptors and Viral Receptors Are Required for Antibody-Dependent Enhancement of Porcine Reproductive and Respiratory Syndrome Virus Infection

doi: 10.3390/vetsci9090470

Figure Lengend Snippet: The sequences of siRNAs.

Article Snippet: The negative control small interfering RNA (siRNA) and the siRNA used for silencing porcine FcγRI or FcγRIII gene (seen in ) were supplied by the Shanghai GenePharma Corporation in China.

Techniques: Sequencing, Negative Control

Journal: Veterinary Sciences

Article Title: Activating Fc Gamma Receptors and Viral Receptors Are Required for Antibody-Dependent Enhancement of Porcine Reproductive and Respiratory Syndrome Virus Infection

doi: 10.3390/vetsci9090470

Figure Lengend Snippet: The sequences of relative quantitative RT-PCR primers.

Article Snippet: The negative control small interfering RNA (siRNA) and the siRNA used for silencing porcine FcγRI or FcγRIII gene (seen in ) were supplied by the Shanghai GenePharma Corporation in China.

Techniques: Quantitative RT-PCR, Sequencing

Journal: Veterinary Sciences

Article Title: Activating Fc Gamma Receptors and Viral Receptors Are Required for Antibody-Dependent Enhancement of Porcine Reproductive and Respiratory Syndrome Virus Infection

doi: 10.3390/vetsci9090470

Figure Lengend Snippet: Analysis of the transcription expression of FcγRI or FcγRIII in PAMs. PAMs cells were transfected with porcine FcγRI or FcγRIII siRNA. The mRNA of FcγRI ( a ) or FcγRIII ( b ) in transfected cells was measured by relative quantitative RT-PCR. ** p < 0.01, * p < 0.05.

Article Snippet: The negative control small interfering RNA (siRNA) and the siRNA used for silencing porcine FcγRI or FcγRIII gene (seen in ) were supplied by the Shanghai GenePharma Corporation in China.

Techniques: Expressing, Transfection, Quantitative RT-PCR

Journal: Veterinary Sciences

Article Title: Activating Fc Gamma Receptors and Viral Receptors Are Required for Antibody-Dependent Enhancement of Porcine Reproductive and Respiratory Syndrome Virus Infection

doi: 10.3390/vetsci9090470

Figure Lengend Snippet: Analysis of the total cellular protein expression of FcγRI or FcγRIII in PAMs. PAMs cells were transfected with porcine FcγRI or FcγRIII siRNA for forty-eight hours and then used for the analysis of immunoblot. The total cellular protein level of FcγRI ( a ) or FcγRIII ( b ) in transfected cells was quantified by Western blot. The original version of the Western blot can be seen in . The GAPDH was used as a reference protein.

Article Snippet: The negative control small interfering RNA (siRNA) and the siRNA used for silencing porcine FcγRI or FcγRIII gene (seen in ) were supplied by the Shanghai GenePharma Corporation in China.

Techniques: Expressing, Transfection, Western Blot

Journal: Veterinary Sciences

Article Title: Activating Fc Gamma Receptors and Viral Receptors Are Required for Antibody-Dependent Enhancement of Porcine Reproductive and Respiratory Syndrome Virus Infection

doi: 10.3390/vetsci9090470

Figure Lengend Snippet: The cytomembrane protein level of FcγRI or FcγRIII on PAMs. PAMs cells were transfected with porcine FcγRI or FcγRIII siRNA for forty-eight hours and then used for the analysis of flow cytometry. ( a ) The cytomembrane protein level of FcγRI on the PAMs cells following negative control siRNA transfection; ( b ) The cytomembrane protein level of FcγRI on the PAMs cells following porcine FcγRI siRNA transfection; ( c ) The cytomembrane protein level of FcγRIII on the PAMs cells following negative control siRNA transfection; ( d ) The cytomembrane protein level of FcγRIII on the PAMs cells following porcine FcγRIII siRNA transfection.

Article Snippet: The negative control small interfering RNA (siRNA) and the siRNA used for silencing porcine FcγRI or FcγRIII gene (seen in ) were supplied by the Shanghai GenePharma Corporation in China.

Techniques: Transfection, Flow Cytometry, Negative Control

Journal: Veterinary Sciences

Article Title: Activating Fc Gamma Receptors and Viral Receptors Are Required for Antibody-Dependent Enhancement of Porcine Reproductive and Respiratory Syndrome Virus Infection

doi: 10.3390/vetsci9090470

Figure Lengend Snippet: Effect of FcγRI or FcγRIII knockdown on ADE activity of PRRSV infection in PAMs. PAMs cells were transfected with porcine FcγRI or FcγRIII siRNA for forty-eight hours and then infected by PRRSV + ICs for the indicated time. The RNA levels ( a ) and the virus titers ( b ) of PRRSV in cell supernatants harvested were evaluated by real-time RT-PCR and TCID 50 assay. *** p < 0.001, ** p < 0.01.

Article Snippet: The negative control small interfering RNA (siRNA) and the siRNA used for silencing porcine FcγRI or FcγRIII gene (seen in ) were supplied by the Shanghai GenePharma Corporation in China.

Techniques: Activity Assay, Infection, Transfection, Quantitative RT-PCR