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    Only available in Europe Triple filtered through 0 1 μm filters Each lot of fetal bovine serum is tested for sterility and for the ability to support the growth of
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    79
    Millipore fbv solution
    Fbv Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 79 stars, based on 3 article reviews
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    76
    The Jackson Laboratory wild type fbv mice
    Wild Type Fbv Mice, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 76/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The Jackson Laboratory b6 fbv tg
    B6 Fbv Tg, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 76/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 76 stars, based on 1 article reviews
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    GE Healthcare blood velocity fbv
    Blood Velocity Fbv, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 76/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 76 stars, based on 2 article reviews
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    79
    Olympus olympus fbv 1000
    Olympus Fbv 1000, supplied by Olympus, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    GE Healthcare fbs fbs
    STAT3/survivin signaling pathway mediates the resistance to ADT-induced apoptosis in DAB2IP-deficient PCa. C4-2 or LAPC-4 KD cells were pretreated with specific STAT3 inhibitor Stattic (10 or 20 μ M) for 1 h or transfected with siRNA oligonucleotides specific to STAT3 or survivin for 24 h, and then cultured in Phenol Red-free <t>RPMI-1640+5%</t> <t>CS-FBS</t> for another 48 h. ( a and b ) Cell lysates after treatment were subjected to western blotting for detecting p-STAT3 (Y705), t-STAT3, survivin, cleaved PARP and Caspase-3. Actin was used as a loading control ( c ) C4-2 cells after treatment were cultured in Phenol Red-free RPMI-1640+5% CS-FBS for another 48 h and cell growth was determined by MTT assay. ( d ) C4-2 cells after treatment were cultured in Phenol Red-free RPMI-1640+5% CS-FBS for another 48 h, then subjected to Annexin V/FITC staining and flow cytometric analysis. ( e ) C4-2 cells after treatment were cultured in Phenol Red-free RPMI-1640+5% CS-FBS for another 48 h, then subjected to JC-1 staining and flow cytometric analysis. All data (means±S.E.M.) shown in c , d and e were obtained from three independent experiments. * P
    Fbs Fbs, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 78/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Charles River Laboratories female fbv mice
    STAT3/survivin signaling pathway mediates the resistance to ADT-induced apoptosis in DAB2IP-deficient PCa. C4-2 or LAPC-4 KD cells were pretreated with specific STAT3 inhibitor Stattic (10 or 20 μ M) for 1 h or transfected with siRNA oligonucleotides specific to STAT3 or survivin for 24 h, and then cultured in Phenol Red-free <t>RPMI-1640+5%</t> <t>CS-FBS</t> for another 48 h. ( a and b ) Cell lysates after treatment were subjected to western blotting for detecting p-STAT3 (Y705), t-STAT3, survivin, cleaved PARP and Caspase-3. Actin was used as a loading control ( c ) C4-2 cells after treatment were cultured in Phenol Red-free RPMI-1640+5% CS-FBS for another 48 h and cell growth was determined by MTT assay. ( d ) C4-2 cells after treatment were cultured in Phenol Red-free RPMI-1640+5% CS-FBS for another 48 h, then subjected to Annexin V/FITC staining and flow cytometric analysis. ( e ) C4-2 cells after treatment were cultured in Phenol Red-free RPMI-1640+5% CS-FBS for another 48 h, then subjected to JC-1 staining and flow cytometric analysis. All data (means±S.E.M.) shown in c , d and e were obtained from three independent experiments. * P
    Female Fbv Mice, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Thermo Fisher • fbs
    STAT3/survivin signaling pathway mediates the resistance to ADT-induced apoptosis in DAB2IP-deficient PCa. C4-2 or LAPC-4 KD cells were pretreated with specific STAT3 inhibitor Stattic (10 or 20 μ M) for 1 h or transfected with siRNA oligonucleotides specific to STAT3 or survivin for 24 h, and then cultured in Phenol Red-free <t>RPMI-1640+5%</t> <t>CS-FBS</t> for another 48 h. ( a and b ) Cell lysates after treatment were subjected to western blotting for detecting p-STAT3 (Y705), t-STAT3, survivin, cleaved PARP and Caspase-3. Actin was used as a loading control ( c ) C4-2 cells after treatment were cultured in Phenol Red-free RPMI-1640+5% CS-FBS for another 48 h and cell growth was determined by MTT assay. ( d ) C4-2 cells after treatment were cultured in Phenol Red-free RPMI-1640+5% CS-FBS for another 48 h, then subjected to Annexin V/FITC staining and flow cytometric analysis. ( e ) C4-2 cells after treatment were cultured in Phenol Red-free RPMI-1640+5% CS-FBS for another 48 h, then subjected to JC-1 staining and flow cytometric analysis. All data (means±S.E.M.) shown in c , d and e were obtained from three independent experiments. * P
    • Fbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    75
    Biological Industries Inc fbs cd fbs
    STAT3/survivin signaling pathway mediates the resistance to ADT-induced apoptosis in DAB2IP-deficient PCa. C4-2 or LAPC-4 KD cells were pretreated with specific STAT3 inhibitor Stattic (10 or 20 μ M) for 1 h or transfected with siRNA oligonucleotides specific to STAT3 or survivin for 24 h, and then cultured in Phenol Red-free <t>RPMI-1640+5%</t> <t>CS-FBS</t> for another 48 h. ( a and b ) Cell lysates after treatment were subjected to western blotting for detecting p-STAT3 (Y705), t-STAT3, survivin, cleaved PARP and Caspase-3. Actin was used as a loading control ( c ) C4-2 cells after treatment were cultured in Phenol Red-free RPMI-1640+5% CS-FBS for another 48 h and cell growth was determined by MTT assay. ( d ) C4-2 cells after treatment were cultured in Phenol Red-free RPMI-1640+5% CS-FBS for another 48 h, then subjected to Annexin V/FITC staining and flow cytometric analysis. ( e ) C4-2 cells after treatment were cultured in Phenol Red-free RPMI-1640+5% CS-FBS for another 48 h, then subjected to JC-1 staining and flow cytometric analysis. All data (means±S.E.M.) shown in c , d and e were obtained from three independent experiments. * P
    Fbs Cd Fbs, supplied by Biological Industries Inc, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Biochrom fbs cs fbs
    STAT3/survivin signaling pathway mediates the resistance to ADT-induced apoptosis in DAB2IP-deficient PCa. C4-2 or LAPC-4 KD cells were pretreated with specific STAT3 inhibitor Stattic (10 or 20 μ M) for 1 h or transfected with siRNA oligonucleotides specific to STAT3 or survivin for 24 h, and then cultured in Phenol Red-free <t>RPMI-1640+5%</t> <t>CS-FBS</t> for another 48 h. ( a and b ) Cell lysates after treatment were subjected to western blotting for detecting p-STAT3 (Y705), t-STAT3, survivin, cleaved PARP and Caspase-3. Actin was used as a loading control ( c ) C4-2 cells after treatment were cultured in Phenol Red-free RPMI-1640+5% CS-FBS for another 48 h and cell growth was determined by MTT assay. ( d ) C4-2 cells after treatment were cultured in Phenol Red-free RPMI-1640+5% CS-FBS for another 48 h, then subjected to Annexin V/FITC staining and flow cytometric analysis. ( e ) C4-2 cells after treatment were cultured in Phenol Red-free RPMI-1640+5% CS-FBS for another 48 h, then subjected to JC-1 staining and flow cytometric analysis. All data (means±S.E.M.) shown in c , d and e were obtained from three independent experiments. * P
    Fbs Cs Fbs, supplied by Biochrom, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher fbv n control mice
    qRT-PCR validation of the GEP microarray data ( blue ) in Sca1+ cells purified from BM or spleen of another set of <t>HR-MDS</t> mice ( two different hues of orange ), and CD34+ cells purified from MDS patients ( green ) compared to <t>FBV/N</t> or CD34 BM cells, respectively. Data expressed as fold change of expression of several genes of three pathways shown (angpt1 (signal transduction); cox4i1 and ndufv2 (oxidative metabolism); sdc1 (DNA processing)). qRT-PCR fold changes shown here are represented as the average of three experiments
    Fbv N Control Mice, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher fbs dmem fbs
    qRT-PCR validation of the GEP microarray data ( blue ) in Sca1+ cells purified from BM or spleen of another set of <t>HR-MDS</t> mice ( two different hues of orange ), and CD34+ cells purified from MDS patients ( green ) compared to <t>FBV/N</t> or CD34 BM cells, respectively. Data expressed as fold change of expression of several genes of three pathways shown (angpt1 (signal transduction); cox4i1 and ndufv2 (oxidative metabolism); sdc1 (DNA processing)). qRT-PCR fold changes shown here are represented as the average of three experiments
    Fbs Dmem Fbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Atlanta Biologicals certified fbs
    qRT-PCR validation of the GEP microarray data ( blue ) in Sca1+ cells purified from BM or spleen of another set of <t>HR-MDS</t> mice ( two different hues of orange ), and CD34+ cells purified from MDS patients ( green ) compared to <t>FBV/N</t> or CD34 BM cells, respectively. Data expressed as fold change of expression of several genes of three pathways shown (angpt1 (signal transduction); cox4i1 and ndufv2 (oxidative metabolism); sdc1 (DNA processing)). qRT-PCR fold changes shown here are represented as the average of three experiments
    Certified Fbs, supplied by Atlanta Biologicals, used in various techniques. Bioz Stars score: 78/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Atlanta Biologicals dialyzed fbs
    qRT-PCR validation of the GEP microarray data ( blue ) in Sca1+ cells purified from BM or spleen of another set of <t>HR-MDS</t> mice ( two different hues of orange ), and CD34+ cells purified from MDS patients ( green ) compared to <t>FBV/N</t> or CD34 BM cells, respectively. Data expressed as fold change of expression of several genes of three pathways shown (angpt1 (signal transduction); cox4i1 and ndufv2 (oxidative metabolism); sdc1 (DNA processing)). qRT-PCR fold changes shown here are represented as the average of three experiments
    Dialyzed Fbs, supplied by Atlanta Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Atlanta Biologicals hi fbs
    qRT-PCR validation of the GEP microarray data ( blue ) in Sca1+ cells purified from BM or spleen of another set of <t>HR-MDS</t> mice ( two different hues of orange ), and CD34+ cells purified from MDS patients ( green ) compared to <t>FBV/N</t> or CD34 BM cells, respectively. Data expressed as fold change of expression of several genes of three pathways shown (angpt1 (signal transduction); cox4i1 and ndufv2 (oxidative metabolism); sdc1 (DNA processing)). qRT-PCR fold changes shown here are represented as the average of three experiments
    Hi Fbs, supplied by Atlanta Biologicals, used in various techniques. Bioz Stars score: 89/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Atlanta Biologicals premium fbs
    qRT-PCR validation of the GEP microarray data ( blue ) in Sca1+ cells purified from BM or spleen of another set of <t>HR-MDS</t> mice ( two different hues of orange ), and CD34+ cells purified from MDS patients ( green ) compared to <t>FBV/N</t> or CD34 BM cells, respectively. Data expressed as fold change of expression of several genes of three pathways shown (angpt1 (signal transduction); cox4i1 and ndufv2 (oxidative metabolism); sdc1 (DNA processing)). qRT-PCR fold changes shown here are represented as the average of three experiments
    Premium Fbs, supplied by Atlanta Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Atlas Biologicals fbs equivalent
    qRT-PCR validation of the GEP microarray data ( blue ) in Sca1+ cells purified from BM or spleen of another set of <t>HR-MDS</t> mice ( two different hues of orange ), and CD34+ cells purified from MDS patients ( green ) compared to <t>FBV/N</t> or CD34 BM cells, respectively. Data expressed as fold change of expression of several genes of three pathways shown (angpt1 (signal transduction); cox4i1 and ndufv2 (oxidative metabolism); sdc1 (DNA processing)). qRT-PCR fold changes shown here are represented as the average of three experiments
    Fbs Equivalent, supplied by Atlas Biologicals, used in various techniques. Bioz Stars score: 80/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Becton Dickinson buffer fbs
    qRT-PCR validation of the GEP microarray data ( blue ) in Sca1+ cells purified from BM or spleen of another set of <t>HR-MDS</t> mice ( two different hues of orange ), and CD34+ cells purified from MDS patients ( green ) compared to <t>FBV/N</t> or CD34 BM cells, respectively. Data expressed as fold change of expression of several genes of three pathways shown (angpt1 (signal transduction); cox4i1 and ndufv2 (oxidative metabolism); sdc1 (DNA processing)). qRT-PCR fold changes shown here are represented as the average of three experiments
    Buffer Fbs, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 89/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cellgro dialyzed fbs
    qRT-PCR validation of the GEP microarray data ( blue ) in Sca1+ cells purified from BM or spleen of another set of <t>HR-MDS</t> mice ( two different hues of orange ), and CD34+ cells purified from MDS patients ( green ) compared to <t>FBV/N</t> or CD34 BM cells, respectively. Data expressed as fold change of expression of several genes of three pathways shown (angpt1 (signal transduction); cox4i1 and ndufv2 (oxidative metabolism); sdc1 (DNA processing)). qRT-PCR fold changes shown here are represented as the average of three experiments
    Dialyzed Fbs, supplied by Cellgro, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fisher Scientific dialyzed fbs
    GAG sulfation is critical for SPICE binding to CHO cells. CHO cells were incubated in sulfate-free Ham’s <t>F12</t> containing dialyzed <t>FBS</t> with 25 mM chlorate, 25 mM chlorate plus 25 mM sulfate, or normal Ham’s F12 (media control). After harvesting
    Dialyzed Fbs, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 95/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Gemini Bio dialyzed fbs
    GAG sulfation is critical for SPICE binding to CHO cells. CHO cells were incubated in sulfate-free Ham’s <t>F12</t> containing dialyzed <t>FBS</t> with 25 mM chlorate, 25 mM chlorate plus 25 mM sulfate, or normal Ham’s F12 (media control). After harvesting
    Dialyzed Fbs, supplied by Gemini Bio, used in various techniques. Bioz Stars score: 95/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Gemini Bio gemcell fbs
    GAG sulfation is critical for SPICE binding to CHO cells. CHO cells were incubated in sulfate-free Ham’s <t>F12</t> containing dialyzed <t>FBS</t> with 25 mM chlorate, 25 mM chlorate plus 25 mM sulfate, or normal Ham’s F12 (media control). After harvesting
    Gemcell Fbs, supplied by Gemini Bio, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Gemini Bio hi fbs
    GAG sulfation is critical for SPICE binding to CHO cells. CHO cells were incubated in sulfate-free Ham’s <t>F12</t> containing dialyzed <t>FBS</t> with 25 mM chlorate, 25 mM chlorate plus 25 mM sulfate, or normal Ham’s F12 (media control). After harvesting
    Hi Fbs, supplied by Gemini Bio, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mediatech fbs media
    GAG sulfation is critical for SPICE binding to CHO cells. CHO cells were incubated in sulfate-free Ham’s <t>F12</t> containing dialyzed <t>FBS</t> with 25 mM chlorate, 25 mM chlorate plus 25 mM sulfate, or normal Ham’s F12 (media control). After harvesting
    Fbs Media, supplied by Mediatech, used in various techniques. Bioz Stars score: 85/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore dialyzed fbs
    GAG sulfation is critical for SPICE binding to CHO cells. CHO cells were incubated in sulfate-free Ham’s <t>F12</t> containing dialyzed <t>FBS</t> with 25 mM chlorate, 25 mM chlorate plus 25 mM sulfate, or normal Ham’s F12 (media control). After harvesting
    Dialyzed Fbs, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 675 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Omega Scientific Inc es fbs
    GAG sulfation is critical for SPICE binding to CHO cells. CHO cells were incubated in sulfate-free Ham’s <t>F12</t> containing dialyzed <t>FBS</t> with 25 mM chlorate, 25 mM chlorate plus 25 mM sulfate, or normal Ham’s F12 (media control). After harvesting
    Es Fbs, supplied by Omega Scientific Inc, used in various techniques. Bioz Stars score: 81/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Silantes GmbH dialyzed fbs
    GAG sulfation is critical for SPICE binding to CHO cells. CHO cells were incubated in sulfate-free Ham’s <t>F12</t> containing dialyzed <t>FBS</t> with 25 mM chlorate, 25 mM chlorate plus 25 mM sulfate, or normal Ham’s F12 (media control). After harvesting
    Dialyzed Fbs, supplied by Silantes GmbH, used in various techniques. Bioz Stars score: 95/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher dcc fbs
    GAG sulfation is critical for SPICE binding to CHO cells. CHO cells were incubated in sulfate-free Ham’s <t>F12</t> containing dialyzed <t>FBS</t> with 25 mM chlorate, 25 mM chlorate plus 25 mM sulfate, or normal Ham’s F12 (media control). After harvesting
    Dcc Fbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher decomplemented fbs
    GAG sulfation is critical for SPICE binding to CHO cells. CHO cells were incubated in sulfate-free Ham’s <t>F12</t> containing dialyzed <t>FBS</t> with 25 mM chlorate, 25 mM chlorate plus 25 mM sulfate, or normal Ham’s F12 (media control). After harvesting
    Decomplemented Fbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dialysed fbs
    c-Jun correlates strongly with GLS levels in human breast cancer cell lines. Cells were collected at ∼60% confluency from <t>RPMI</t> growth medium supplemented with 10% <t>FBS,</t> and whole-cell lysates prepared and analysed by western blot. Samples were ordered according to glutamine dependence, increasing from left to right ( Supplementary Fig. 3 ). ( a ) Correlation between c-Jun/phospho-c-Jun and GLS levels. Quantification of GLS and c-Jun band intensities allowed a Pearson correlation coefficient of 0.85 to be determined ( Supplementary Fig. 4a ). ( b ) Other Jun-family members do not correlate strongly with GLS levels. ( c ) Under 10% FBS culture conditions, p46 JNK (lower band) is active in all of the breast cancer cell lines. Neither JNK nor phospho-JNK correlate with GLS levels. ( d ) Other reported regulators of GLS expression do not strongly correlate with GLS levels.
    Dialysed Fbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 109 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    STAT3/survivin signaling pathway mediates the resistance to ADT-induced apoptosis in DAB2IP-deficient PCa. C4-2 or LAPC-4 KD cells were pretreated with specific STAT3 inhibitor Stattic (10 or 20 μ M) for 1 h or transfected with siRNA oligonucleotides specific to STAT3 or survivin for 24 h, and then cultured in Phenol Red-free RPMI-1640+5% CS-FBS for another 48 h. ( a and b ) Cell lysates after treatment were subjected to western blotting for detecting p-STAT3 (Y705), t-STAT3, survivin, cleaved PARP and Caspase-3. Actin was used as a loading control ( c ) C4-2 cells after treatment were cultured in Phenol Red-free RPMI-1640+5% CS-FBS for another 48 h and cell growth was determined by MTT assay. ( d ) C4-2 cells after treatment were cultured in Phenol Red-free RPMI-1640+5% CS-FBS for another 48 h, then subjected to Annexin V/FITC staining and flow cytometric analysis. ( e ) C4-2 cells after treatment were cultured in Phenol Red-free RPMI-1640+5% CS-FBS for another 48 h, then subjected to JC-1 staining and flow cytometric analysis. All data (means±S.E.M.) shown in c , d and e were obtained from three independent experiments. * P

    Journal: Cell Death & Disease

    Article Title: DAB2IP loss confers the resistance of prostate cancer to androgen deprivation therapy through activating STAT3 and inhibiting apoptosis

    doi: 10.1038/cddis.2015.289

    Figure Lengend Snippet: STAT3/survivin signaling pathway mediates the resistance to ADT-induced apoptosis in DAB2IP-deficient PCa. C4-2 or LAPC-4 KD cells were pretreated with specific STAT3 inhibitor Stattic (10 or 20 μ M) for 1 h or transfected with siRNA oligonucleotides specific to STAT3 or survivin for 24 h, and then cultured in Phenol Red-free RPMI-1640+5% CS-FBS for another 48 h. ( a and b ) Cell lysates after treatment were subjected to western blotting for detecting p-STAT3 (Y705), t-STAT3, survivin, cleaved PARP and Caspase-3. Actin was used as a loading control ( c ) C4-2 cells after treatment were cultured in Phenol Red-free RPMI-1640+5% CS-FBS for another 48 h and cell growth was determined by MTT assay. ( d ) C4-2 cells after treatment were cultured in Phenol Red-free RPMI-1640+5% CS-FBS for another 48 h, then subjected to Annexin V/FITC staining and flow cytometric analysis. ( e ) C4-2 cells after treatment were cultured in Phenol Red-free RPMI-1640+5% CS-FBS for another 48 h, then subjected to JC-1 staining and flow cytometric analysis. All data (means±S.E.M.) shown in c , d and e were obtained from three independent experiments. * P

    Article Snippet: To deplete androgen, cells were sub-cultured in Phenol Red-free RPMI-1640 with 5% or 10% CS-FBS (Hyclone, Omaha, NE, USA).

    Techniques: Transfection, Cell Culture, Western Blot, MTT Assay, Staining, Flow Cytometry

    DAB2IP controls the intrinsic apoptosis of PCa cells following ADT. PCa sublines were cultured in Phenol Red-free RPMI-1640+5% CS-FBS for 3 days. ( a ) C4-2 (left) and LAPC-4 (right) sublines were then collected for Annexin V/FITC staining and flow cytometric analysis, and data (means±S.E.M.) were obtained from three independent experiments; * P

    Journal: Cell Death & Disease

    Article Title: DAB2IP loss confers the resistance of prostate cancer to androgen deprivation therapy through activating STAT3 and inhibiting apoptosis

    doi: 10.1038/cddis.2015.289

    Figure Lengend Snippet: DAB2IP controls the intrinsic apoptosis of PCa cells following ADT. PCa sublines were cultured in Phenol Red-free RPMI-1640+5% CS-FBS for 3 days. ( a ) C4-2 (left) and LAPC-4 (right) sublines were then collected for Annexin V/FITC staining and flow cytometric analysis, and data (means±S.E.M.) were obtained from three independent experiments; * P

    Article Snippet: To deplete androgen, cells were sub-cultured in Phenol Red-free RPMI-1640 with 5% or 10% CS-FBS (Hyclone, Omaha, NE, USA).

    Techniques: Cell Culture, Staining, Flow Cytometry

    DAB2IP modulates the cell survival of PCa cells under androgen-depleted condition. ( a – c ) C4-2 and LAPC-4 sublines were cultured in Phenol Red-free RPMI-1640+5% CS-FBS and cell growth was determined by MTT or colony formation assay; data (means±S.E.M.) were obtained from three independent experiments; * P

    Journal: Cell Death & Disease

    Article Title: DAB2IP loss confers the resistance of prostate cancer to androgen deprivation therapy through activating STAT3 and inhibiting apoptosis

    doi: 10.1038/cddis.2015.289

    Figure Lengend Snippet: DAB2IP modulates the cell survival of PCa cells under androgen-depleted condition. ( a – c ) C4-2 and LAPC-4 sublines were cultured in Phenol Red-free RPMI-1640+5% CS-FBS and cell growth was determined by MTT or colony formation assay; data (means±S.E.M.) were obtained from three independent experiments; * P

    Article Snippet: To deplete androgen, cells were sub-cultured in Phenol Red-free RPMI-1640 with 5% or 10% CS-FBS (Hyclone, Omaha, NE, USA).

    Techniques: Cell Culture, MTT Assay, Colony Assay

    Cells maintained in EV-depleted serum exhibit reduced cell growth. (A) 150,000 cells were plated in a 5 cm flask and counted again after the indicated times. The 24-hour proliferation rate was calculated. Each cell type is normalized to its control, which is indicated by the gray line (n = 4 for the sSY5Y and HeLa cells; n = 7 for the other cell lines). (B) Cells were grown for 48 hours with regular 10% FBS, EV-depleted 10% FBS, or EV-depleted 10% FBS with isolated FBS EVs. Cell viability was measured by MTS assay (n = 6). (C) Using the same experimental design as in (B), MTS assays were performed on HEK, N2a, and U87 cells incubated in DMEM containing control and EV-depleted FBS from ATCC and Gibco as well as from Hyclone. (D) The indicated cell types were grown for 48 hours with 10% human serum or EV-depleted human serum. Viability was measured by MTS assay (n = 6). *p

    Journal: Journal of Extracellular Vesicles

    Article Title: Extracellular vesicle–depleted fetal bovine and human sera have reduced capacity to support cell growth

    doi: 10.3402/jev.v4.26373

    Figure Lengend Snippet: Cells maintained in EV-depleted serum exhibit reduced cell growth. (A) 150,000 cells were plated in a 5 cm flask and counted again after the indicated times. The 24-hour proliferation rate was calculated. Each cell type is normalized to its control, which is indicated by the gray line (n = 4 for the sSY5Y and HeLa cells; n = 7 for the other cell lines). (B) Cells were grown for 48 hours with regular 10% FBS, EV-depleted 10% FBS, or EV-depleted 10% FBS with isolated FBS EVs. Cell viability was measured by MTS assay (n = 6). (C) Using the same experimental design as in (B), MTS assays were performed on HEK, N2a, and U87 cells incubated in DMEM containing control and EV-depleted FBS from ATCC and Gibco as well as from Hyclone. (D) The indicated cell types were grown for 48 hours with 10% human serum or EV-depleted human serum. Viability was measured by MTS assay (n = 6). *p

    Article Snippet: These results were consistent among 4 different batches of Hyclone FBS and when the FBS was purchased from ATCC or Gibco ( C and Supplementary Fig. 1).

    Techniques: Isolation, MTS Assay, Incubation

    qRT-PCR validation of the GEP microarray data ( blue ) in Sca1+ cells purified from BM or spleen of another set of HR-MDS mice ( two different hues of orange ), and CD34+ cells purified from MDS patients ( green ) compared to FBV/N or CD34 BM cells, respectively. Data expressed as fold change of expression of several genes of three pathways shown (angpt1 (signal transduction); cox4i1 and ndufv2 (oxidative metabolism); sdc1 (DNA processing)). qRT-PCR fold changes shown here are represented as the average of three experiments

    Journal: Journal of Hematology & Oncology

    Article Title: GEP analysis validates high risk MDS and acute myeloid leukemia post MDS mice models and highlights novel dysregulated pathways

    doi: 10.1186/s13045-016-0235-8

    Figure Lengend Snippet: qRT-PCR validation of the GEP microarray data ( blue ) in Sca1+ cells purified from BM or spleen of another set of HR-MDS mice ( two different hues of orange ), and CD34+ cells purified from MDS patients ( green ) compared to FBV/N or CD34 BM cells, respectively. Data expressed as fold change of expression of several genes of three pathways shown (angpt1 (signal transduction); cox4i1 and ndufv2 (oxidative metabolism); sdc1 (DNA processing)). qRT-PCR fold changes shown here are represented as the average of three experiments

    Article Snippet: Affymetrix exon array hybridization One microgram of total RNA extracted from Sca1+ spleen cells from the AML post MDS mice, HR-MDS mice, single-transgenic mice necessary to produce these AML post MDS and HR-MDS mice, (MRP8 NRASD12, MRP8 hBCL-2, tet hBCL-2) (called founder mice), and FBV/N control mice (n = 3 each) was labeled with Affymetrix reagents and hybridized to Affymetrix-GeneChip Mouse Exon 1.0 ST arrays.

    Techniques: Quantitative RT-PCR, Microarray, Purification, Mouse Assay, Expressing, Transduction

    GAG sulfation is critical for SPICE binding to CHO cells. CHO cells were incubated in sulfate-free Ham’s F12 containing dialyzed FBS with 25 mM chlorate, 25 mM chlorate plus 25 mM sulfate, or normal Ham’s F12 (media control). After harvesting

    Journal:

    Article Title: Smallpox inhibitor of complement enzymes (SPICE): Regulation of complement activation on cells and mechanism of its cellular attachment

    doi:

    Figure Lengend Snippet: GAG sulfation is critical for SPICE binding to CHO cells. CHO cells were incubated in sulfate-free Ham’s F12 containing dialyzed FBS with 25 mM chlorate, 25 mM chlorate plus 25 mM sulfate, or normal Ham’s F12 (media control). After harvesting

    Article Snippet: CHO cells were cultured in sulfate-free Ham’s F12 medium (Washington University Tissue Culture Support Center) supplemented with 10% dialyzed FBS (Fisher Scientific) containing different concentrations (1–25 mM) of sodium chlorate.

    Techniques: Binding Assay, Incubation

    c-Jun correlates strongly with GLS levels in human breast cancer cell lines. Cells were collected at ∼60% confluency from RPMI growth medium supplemented with 10% FBS, and whole-cell lysates prepared and analysed by western blot. Samples were ordered according to glutamine dependence, increasing from left to right ( Supplementary Fig. 3 ). ( a ) Correlation between c-Jun/phospho-c-Jun and GLS levels. Quantification of GLS and c-Jun band intensities allowed a Pearson correlation coefficient of 0.85 to be determined ( Supplementary Fig. 4a ). ( b ) Other Jun-family members do not correlate strongly with GLS levels. ( c ) Under 10% FBS culture conditions, p46 JNK (lower band) is active in all of the breast cancer cell lines. Neither JNK nor phospho-JNK correlate with GLS levels. ( d ) Other reported regulators of GLS expression do not strongly correlate with GLS levels.

    Journal: Nature Communications

    Article Title: The oncogenic transcription factor c-Jun regulates glutaminase expression and sensitizes cells to glutaminase-targeted therapy

    doi: 10.1038/ncomms11321

    Figure Lengend Snippet: c-Jun correlates strongly with GLS levels in human breast cancer cell lines. Cells were collected at ∼60% confluency from RPMI growth medium supplemented with 10% FBS, and whole-cell lysates prepared and analysed by western blot. Samples were ordered according to glutamine dependence, increasing from left to right ( Supplementary Fig. 3 ). ( a ) Correlation between c-Jun/phospho-c-Jun and GLS levels. Quantification of GLS and c-Jun band intensities allowed a Pearson correlation coefficient of 0.85 to be determined ( Supplementary Fig. 4a ). ( b ) Other Jun-family members do not correlate strongly with GLS levels. ( c ) Under 10% FBS culture conditions, p46 JNK (lower band) is active in all of the breast cancer cell lines. Neither JNK nor phospho-JNK correlate with GLS levels. ( d ) Other reported regulators of GLS expression do not strongly correlate with GLS levels.

    Article Snippet: For glutamine-withdrawal experiments, glutamine-free RPMI 1640 medium (Gibco) supplemented with 10% dialysed FBS (Gibco) was used.

    Techniques: Western Blot, Expressing