fatty acid-free bovine serum albumin bsa Search Results


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  • 99
    Thermo Fisher fatty acid free bovine serum albumin bsa
    Fatty Acid Free Bovine Serum Albumin Bsa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fatty acid free bovine serum albumin bsa/product/Thermo Fisher
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    Millipore fatty acid free bovine serum albumin bsa
    Glutathionation in A549 cells supplemented with <t>BSA-conjugated</t> fatty acids (LA, DHA, BSA carrier control). Cells were treated with BSO or <t>GSH</t> in combination with each of the fatty acids for 48 µM BaP for 24 h. Data represents mean normalized intensity ± SEM of at least 30 images per treatment and 30 cells per image. * indicates significant difference from the corresponding control using Tukey test at p
    Fatty Acid Free Bovine Serum Albumin Bsa, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2877 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fatty acid free bovine serum albumin bsa/product/Millipore
    Average 99 stars, based on 2877 article reviews
    Price from $9.99 to $1999.99
    fatty acid free bovine serum albumin bsa - by Bioz Stars, 2020-08
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    99
    Thermo Fisher fatty acid free bovine serum albumin
    Glutathionation in A549 cells supplemented with <t>BSA-conjugated</t> fatty acids (LA, DHA, BSA carrier control). Cells were treated with BSO or <t>GSH</t> in combination with each of the fatty acids for 48 µM BaP for 24 h. Data represents mean normalized intensity ± SEM of at least 30 images per treatment and 30 cells per image. * indicates significant difference from the corresponding control using Tukey test at p
    Fatty Acid Free Bovine Serum Albumin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fatty acid free bovine serum albumin/product/Thermo Fisher
    Average 99 stars, based on 57 article reviews
    Price from $9.99 to $1999.99
    fatty acid free bovine serum albumin - by Bioz Stars, 2020-08
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    Millipore fatty acid depleted bovine serum albumin bsa
    Infrared differential spectrum of <t>resveratrol</t> with <t>BSA</t> (the spectrum of trans resveratrol with BSA minus the spectrum of cis resveratrol with BSA) obtained after photo-isomerization in the same infrared cell.
    Fatty Acid Depleted Bovine Serum Albumin Bsa, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fatty acid depleted bovine serum albumin bsa/product/Millipore
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    93
    Equitech-Bio fatty acid free bovine serum albumin bsa
    Effect of 24 hour exposure to fatty acids on human BMMSC energy metabolism. A) Palmitate oxidation, B) palmitate uptake, C) glycolysis, and D) glucose oxidation were measured in human <t>BMMSCs</t> that had been treated for 24 hr with either 0.55 mM albumin <t>(BSA</t> group) or 0.55 mM albumin and 0.4 mM palmitate and/or 0.4 mM oleate prior to these metabolism measurements being made. n = 5–8 The graphs indicate which groups were exposed to these different treatments for the 24 hr prior to the metabolism measurements. During each assay all groups were given Krebs buffer supplemented with 5 mM glucose and 0.4 mM palmitate bound to 0.55 mM albumin. In addition, the Krebs buffer was supplemented with either [U- 14 C]glucose, [1– 14 C]palmitate, or [5– 3 H]glucose for the measurement of glucose oxidation, palmitate oxidation and uptake, or glycolysis, respectively. E) The contribution of metabolic pathways to ATP production were calculated from the metabolic rate results. * Significantly different from all groups. Values are shown as mean ± SEM.
    Fatty Acid Free Bovine Serum Albumin Bsa, supplied by Equitech-Bio, used in various techniques. Bioz Stars score: 93/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche fatty acid free bovine serum albumin bsa
    p21-dependent inhibition of <t>LPA-induced</t> cell proliferation by TGFβ. A . TGFβ inhibits LPA-afforded cell proliferation. MDA-MB-231 and Caov-3 cells in 6-well plates were incubated with LPA (10 µM) or vehicle <t>(BSA)</t> in the presence
    Fatty Acid Free Bovine Serum Albumin Bsa, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore bsa fatty acid free
    p21-dependent inhibition of <t>LPA-induced</t> cell proliferation by TGFβ. A . TGFβ inhibits LPA-afforded cell proliferation. MDA-MB-231 and Caov-3 cells in 6-well plates were incubated with LPA (10 µM) or vehicle <t>(BSA)</t> in the presence
    Bsa Fatty Acid Free, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam fatty acid free bovine serum albumin bsa
    p21-dependent inhibition of <t>LPA-induced</t> cell proliferation by TGFβ. A . TGFβ inhibits LPA-afforded cell proliferation. MDA-MB-231 and Caov-3 cells in 6-well plates were incubated with LPA (10 µM) or vehicle <t>(BSA)</t> in the presence
    Fatty Acid Free Bovine Serum Albumin Bsa, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fatty acid free bovine serum albumin bsa/product/Abcam
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    92
    Boehringer Mannheim fatty acid free bovine serum albumin bsa
    p21-dependent inhibition of <t>LPA-induced</t> cell proliferation by TGFβ. A . TGFβ inhibits LPA-afforded cell proliferation. MDA-MB-231 and Caov-3 cells in 6-well plates were incubated with LPA (10 µM) or vehicle <t>(BSA)</t> in the presence
    Fatty Acid Free Bovine Serum Albumin Bsa, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fatty acid free bovine serum albumin bsa/product/Boehringer Mannheim
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    Image Search Results


    Glutathionation in A549 cells supplemented with BSA-conjugated fatty acids (LA, DHA, BSA carrier control). Cells were treated with BSO or GSH in combination with each of the fatty acids for 48 µM BaP for 24 h. Data represents mean normalized intensity ± SEM of at least 30 images per treatment and 30 cells per image. * indicates significant difference from the corresponding control using Tukey test at p

    Journal: PLoS ONE

    Article Title: Effects of Fatty Acids on Benzo[a]pyrene Uptake and Metabolism in Human Lung Adenocarcinoma A549 Cells

    doi: 10.1371/journal.pone.0090908

    Figure Lengend Snippet: Glutathionation in A549 cells supplemented with BSA-conjugated fatty acids (LA, DHA, BSA carrier control). Cells were treated with BSO or GSH in combination with each of the fatty acids for 48 µM BaP for 24 h. Data represents mean normalized intensity ± SEM of at least 30 images per treatment and 30 cells per image. * indicates significant difference from the corresponding control using Tukey test at p

    Article Snippet: Materials Dulbecco's Modified Eagle's Medium/Ham's Nutrient Mixture F-12 (DMEM-F12), fatty acid free bovine serum albumin (BSA), Dulbecco's phosphate buffered saline (PBS), glutathione (GSH), benzo[a]pyrene (BaP), L- buthionine sulfoximine (BSO), janus green, β-glucuronidase (keyhole limpet, #G8132), triclosan (Irgasan), resorufin, ethyl ether, 3,3′-methylene-bis(4-hydroxycoumarin) (dicumarol), and trans-3,4′,-5-trihydroxystilebene; 3,4′,5-stilbenetriol (resveratrol) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO).

    Techniques:

    Modulation of S1P homeostasis of K562 cells by sodium butyrate-induced differentiation into erythroblast-like cells. K562 cells were treated with 2 mM sodium butyrate (NaB); 72 hours later, we investigated the modulation of S1P homeostasis. (A) The mRNA levels of GYPA and ALAS were determined using real-time PCR. GAPDH was utilized as an internal control (n = 8/group). (B, C) The expression of CD235a was investigated with a flow cytometer (n = 3/group). (D) C 17 S1P formation assay. NaB-treated or vehicle-treated K562 cells were treated with 10 μM of C 17 sphingosine for 20 minutes. Then, we replaced the supernatant with PBS containing 0.5% BSA and incubated the cells at 37°C for another 20 minutes. Then, the supernatants and cells were collected and used for the C 17 S1P measurements (n = 6/group). (E) The expression of key enzymes in S1P metabolism was determined using real-time PCR. GAPDH was utilized as an internal control (n = 8/group). (F) SK activity assay. The SK activity assay was performed using NaB-treated or vehicle-treated K562 cells (n = 6/group). (G) Reverse transcription PCR was performed using cDNAs prepared from NaB-treated K562 cells (K), HepG2 cells (He), and HUVECs (Hu). (H) The expression of possible S1P transporters was determined using real-time PCR. GAPDH was utilized as an internal control (n = 4/group). (I) Real-time PCR of Band3. GAPDH was utilized as an internal control (n = 8/group). (J) Western blot of Band3 with membranous protein. The whole cell lysate of RBCs (2 μg) was placed as a positive control. Pan-cadherin was utilized as an internal control (n = 3/group).

    Journal: PLoS ONE

    Article Title: Involvement of Band3 in the efflux of sphingosine 1-phosphate from erythrocytes

    doi: 10.1371/journal.pone.0177543

    Figure Lengend Snippet: Modulation of S1P homeostasis of K562 cells by sodium butyrate-induced differentiation into erythroblast-like cells. K562 cells were treated with 2 mM sodium butyrate (NaB); 72 hours later, we investigated the modulation of S1P homeostasis. (A) The mRNA levels of GYPA and ALAS were determined using real-time PCR. GAPDH was utilized as an internal control (n = 8/group). (B, C) The expression of CD235a was investigated with a flow cytometer (n = 3/group). (D) C 17 S1P formation assay. NaB-treated or vehicle-treated K562 cells were treated with 10 μM of C 17 sphingosine for 20 minutes. Then, we replaced the supernatant with PBS containing 0.5% BSA and incubated the cells at 37°C for another 20 minutes. Then, the supernatants and cells were collected and used for the C 17 S1P measurements (n = 6/group). (E) The expression of key enzymes in S1P metabolism was determined using real-time PCR. GAPDH was utilized as an internal control (n = 8/group). (F) SK activity assay. The SK activity assay was performed using NaB-treated or vehicle-treated K562 cells (n = 6/group). (G) Reverse transcription PCR was performed using cDNAs prepared from NaB-treated K562 cells (K), HepG2 cells (He), and HUVECs (Hu). (H) The expression of possible S1P transporters was determined using real-time PCR. GAPDH was utilized as an internal control (n = 4/group). (I) Real-time PCR of Band3. GAPDH was utilized as an internal control (n = 8/group). (J) Western blot of Band3 with membranous protein. The whole cell lysate of RBCs (2 μg) was placed as a positive control. Pan-cadherin was utilized as an internal control (n = 3/group).

    Article Snippet: Then, we replaced the supernatant with PBS containing 0.5% fatty acid-free BSA (A8806; Sigma-Aldrich Co.) and incubated the cells at 37°C for another 20 minutes.

    Techniques: Real-time Polymerase Chain Reaction, Expressing, Flow Cytometry, Cytometry, Tube Formation Assay, Incubation, Activity Assay, Polymerase Chain Reaction, Western Blot, Positive Control

    Effects of OF and CM on the mRNA expressions of lipogenesis-related genes. Real-time RT-PCR analysis of sterol regulatory element-binding protein-1 ( SREBP-1c ) (a) and glycerol-3-phosphate acyltransferase ( GPAT ) (b) mRNA levels in 1 mM FFA/BSA-treated HepG2 cells. All data were normalized to GAPDH mRNA, and the fold changes in expression were calculated relative to control cells (treated with 1% BSA). Each experiment was independently performed three times. Data were presented as the mean ± SD. Values not sharing common superscripts are significantly different ( p

    Journal: Mediators of Inflammation

    Article Title: Antilipotoxicity Activity of Osmanthus fragrans and Chrysanthemum morifolium Flower Extracts in Hepatocytes and Renal Glomerular Mesangial Cells

    doi: 10.1155/2017/4856095

    Figure Lengend Snippet: Effects of OF and CM on the mRNA expressions of lipogenesis-related genes. Real-time RT-PCR analysis of sterol regulatory element-binding protein-1 ( SREBP-1c ) (a) and glycerol-3-phosphate acyltransferase ( GPAT ) (b) mRNA levels in 1 mM FFA/BSA-treated HepG2 cells. All data were normalized to GAPDH mRNA, and the fold changes in expression were calculated relative to control cells (treated with 1% BSA). Each experiment was independently performed three times. Data were presented as the mean ± SD. Values not sharing common superscripts are significantly different ( p

    Article Snippet: The FFA/BSA or oleic acid (OA)/BSA complex solution was sterile-filtered through 0.22 μ m sterile filters (Millipore S.A.S., Molsheim, France) and then stored at −20°C until use.

    Techniques: Quantitative RT-PCR, Binding Assay, Expressing

    Effect of OF and CM on FFA-induced ROS production. HepG2 cells were incubated with 1 mM FFAs/BSA for 24 h in the presence of OF or CM. Vehicle cells were incubated with 0.1% DMSO in the presence of FFAs/BSA. Control cells were incubated with 1% BSA. Intracellular ROS production was quantified using the fluorescent probe DCFDA. Data were presented as mean ± SD of three independent experiments. Values not sharing common superscripts are significantly different ( p

    Journal: Mediators of Inflammation

    Article Title: Antilipotoxicity Activity of Osmanthus fragrans and Chrysanthemum morifolium Flower Extracts in Hepatocytes and Renal Glomerular Mesangial Cells

    doi: 10.1155/2017/4856095

    Figure Lengend Snippet: Effect of OF and CM on FFA-induced ROS production. HepG2 cells were incubated with 1 mM FFAs/BSA for 24 h in the presence of OF or CM. Vehicle cells were incubated with 0.1% DMSO in the presence of FFAs/BSA. Control cells were incubated with 1% BSA. Intracellular ROS production was quantified using the fluorescent probe DCFDA. Data were presented as mean ± SD of three independent experiments. Values not sharing common superscripts are significantly different ( p

    Article Snippet: The FFA/BSA or oleic acid (OA)/BSA complex solution was sterile-filtered through 0.22 μ m sterile filters (Millipore S.A.S., Molsheim, France) and then stored at −20°C until use.

    Techniques: Incubation

    Effects of OF and CM on lipid accumulation in free fatty acid-overloaded HepG2 cells. HepG2 cells were incubated with 1 mM FFAs/BSA and cotreated with various concentrations of OF and CM for 24 h. Vehicle cells were incubated with 0.1% DMSO in the presence of FFAs/BSA. Control cells were incubated with 1% BSA. Cell viability was measured by the MTT assay (a). Quantitative analysis of lipid deposition in cells by the OD 500 nm values using Oil Red O staining (b). Intracellular triglyceride (c) and cholesterol (d) contents were determined in cell lysates by an enzymatic colorimetric method using a commercially available kit. Total cholesterol and TG levels of the control cells were 25.3 ± 7.1 and 28.7 ± 2.7 μ g/mg of cellular protein, respectively. Data were presented as mean ± SD of three independent experiments. Values not sharing common superscripts are significantly different ( p

    Journal: Mediators of Inflammation

    Article Title: Antilipotoxicity Activity of Osmanthus fragrans and Chrysanthemum morifolium Flower Extracts in Hepatocytes and Renal Glomerular Mesangial Cells

    doi: 10.1155/2017/4856095

    Figure Lengend Snippet: Effects of OF and CM on lipid accumulation in free fatty acid-overloaded HepG2 cells. HepG2 cells were incubated with 1 mM FFAs/BSA and cotreated with various concentrations of OF and CM for 24 h. Vehicle cells were incubated with 0.1% DMSO in the presence of FFAs/BSA. Control cells were incubated with 1% BSA. Cell viability was measured by the MTT assay (a). Quantitative analysis of lipid deposition in cells by the OD 500 nm values using Oil Red O staining (b). Intracellular triglyceride (c) and cholesterol (d) contents were determined in cell lysates by an enzymatic colorimetric method using a commercially available kit. Total cholesterol and TG levels of the control cells were 25.3 ± 7.1 and 28.7 ± 2.7 μ g/mg of cellular protein, respectively. Data were presented as mean ± SD of three independent experiments. Values not sharing common superscripts are significantly different ( p

    Article Snippet: The FFA/BSA or oleic acid (OA)/BSA complex solution was sterile-filtered through 0.22 μ m sterile filters (Millipore S.A.S., Molsheim, France) and then stored at −20°C until use.

    Techniques: Incubation, MTT Assay, Staining

    HPN improves glucose uptake ability of HepG2 cells. ( A ) Insulin-stimulated pAkt (S473) was up-regulated during HPN treatment in PA-induced HepG2 cells. HepG2 cells were serum-starved with 0.5% FFA-free BSA medium, and treated with 0.25 mM PA for 16 h after HPN incubation. Subsequently, cells were stimulated with 100 nM insulin for 30 min. Western blot assay was used to determine the changes of pAkt (S473) and total Akt. β-Actin was used as loading control. ### p

    Journal: Marine Drugs

    Article Title: Marine Bromophenol Derivative 3,4-Dibromo-5-(2-bromo-3,4-dihydroxy-6-isopropoxymethyl benzyl)benzene-1,2-diol Protects Hepatocytes from Lipid-Induced Cell Damage and Insulin Resistance via PTP1B Inhibition

    doi: 10.3390/md13074452

    Figure Lengend Snippet: HPN improves glucose uptake ability of HepG2 cells. ( A ) Insulin-stimulated pAkt (S473) was up-regulated during HPN treatment in PA-induced HepG2 cells. HepG2 cells were serum-starved with 0.5% FFA-free BSA medium, and treated with 0.25 mM PA for 16 h after HPN incubation. Subsequently, cells were stimulated with 100 nM insulin for 30 min. Western blot assay was used to determine the changes of pAkt (S473) and total Akt. β-Actin was used as loading control. ### p

    Article Snippet: Palmitate, insulin and FFA-free BSA were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Incubation, Western Blot

    Infrared differential spectrum of resveratrol with BSA (the spectrum of trans resveratrol with BSA minus the spectrum of cis resveratrol with BSA) obtained after photo-isomerization in the same infrared cell.

    Journal: Molecules

    Article Title: Compared Binding Properties between Resveratrol and Other Polyphenols to Plasmatic Albumin: Consequences for the Health Protecting Effect of Dietary Plant Microcomponents

    doi: 10.3390/molecules191117066

    Figure Lengend Snippet: Infrared differential spectrum of resveratrol with BSA (the spectrum of trans resveratrol with BSA minus the spectrum of cis resveratrol with BSA) obtained after photo-isomerization in the same infrared cell.

    Article Snippet: Chemicals Trans -resveratrol, fatty acid-free BSA (bovine serum albumin) and fatty acid-containing BSA (Fraction V) were purchased from Sigma (St. Louis, MO, USA).

    Techniques:

    Effect of 24 hour exposure to fatty acids on human BMMSC energy metabolism. A) Palmitate oxidation, B) palmitate uptake, C) glycolysis, and D) glucose oxidation were measured in human BMMSCs that had been treated for 24 hr with either 0.55 mM albumin (BSA group) or 0.55 mM albumin and 0.4 mM palmitate and/or 0.4 mM oleate prior to these metabolism measurements being made. n = 5–8 The graphs indicate which groups were exposed to these different treatments for the 24 hr prior to the metabolism measurements. During each assay all groups were given Krebs buffer supplemented with 5 mM glucose and 0.4 mM palmitate bound to 0.55 mM albumin. In addition, the Krebs buffer was supplemented with either [U- 14 C]glucose, [1– 14 C]palmitate, or [5– 3 H]glucose for the measurement of glucose oxidation, palmitate oxidation and uptake, or glycolysis, respectively. E) The contribution of metabolic pathways to ATP production were calculated from the metabolic rate results. * Significantly different from all groups. Values are shown as mean ± SEM.

    Journal: PLoS ONE

    Article Title: Effect of Fatty Acids on Human Bone Marrow Mesenchymal Stem Cell Energy Metabolism and Survival

    doi: 10.1371/journal.pone.0120257

    Figure Lengend Snippet: Effect of 24 hour exposure to fatty acids on human BMMSC energy metabolism. A) Palmitate oxidation, B) palmitate uptake, C) glycolysis, and D) glucose oxidation were measured in human BMMSCs that had been treated for 24 hr with either 0.55 mM albumin (BSA group) or 0.55 mM albumin and 0.4 mM palmitate and/or 0.4 mM oleate prior to these metabolism measurements being made. n = 5–8 The graphs indicate which groups were exposed to these different treatments for the 24 hr prior to the metabolism measurements. During each assay all groups were given Krebs buffer supplemented with 5 mM glucose and 0.4 mM palmitate bound to 0.55 mM albumin. In addition, the Krebs buffer was supplemented with either [U- 14 C]glucose, [1– 14 C]palmitate, or [5– 3 H]glucose for the measurement of glucose oxidation, palmitate oxidation and uptake, or glycolysis, respectively. E) The contribution of metabolic pathways to ATP production were calculated from the metabolic rate results. * Significantly different from all groups. Values are shown as mean ± SEM.

    Article Snippet: During experiments assessing the chronic effect of fatty acids on BMMSCs this media was also supplemented with 4% fatty acid free bovine serum albumin (BSA) (Equitech-Bio Inc BAH66) or 4% BSA bound to the indicated type and concentration of fatty acid (Palmitate, Sigma P9767; Oleate, Fluka Analytical 60420; Stearate, Sigma S3381).

    Techniques:

    Effect of acute exposure to fatty acids on human BMMSC energy metabolism. A) Glycolysis, B) glucose oxidation, C) palmitate oxidation, D) palmitate uptake, E) oleate oxidation, and F) oleate uptake were measured in untreated human bone marrow mesenchymal stem cells (BMMSCs). n = 5–7 During each assay Krebs Henseleit buffer was supplemented with 5 mM glucose and, as indicated in each graph, either 0.55 mM albumin (BSA group) or 0.55 mM albumin bound to 0.4 mM palmitate and/or 0.4 mM oleate. In addition, the Krebs Henseleit buffer was supplemented with either [U- 14 C]glucose, [1– 14 C]palmitate, [1– 14 C]oleate, or [5– 3 H]glucose for the measurement of glucose oxidation, palmitate oxidation and uptake, oleate oxidation and uptake, or glycolysis, respectively. Since these experiments assessed the acute effect of palmitate and oleate, BMMSCs were maintained in cell culture media used to culture these immediately up to the start of each assay when the media was switched to Krebs Henseleit buffer supplemented with fatty acids. The levels and type of fatty acid BMMSCs were exposed to is indicated on the x-axis of the figures. * Significantly different from all groups. Values are shown as the mean ± SEM.

    Journal: PLoS ONE

    Article Title: Effect of Fatty Acids on Human Bone Marrow Mesenchymal Stem Cell Energy Metabolism and Survival

    doi: 10.1371/journal.pone.0120257

    Figure Lengend Snippet: Effect of acute exposure to fatty acids on human BMMSC energy metabolism. A) Glycolysis, B) glucose oxidation, C) palmitate oxidation, D) palmitate uptake, E) oleate oxidation, and F) oleate uptake were measured in untreated human bone marrow mesenchymal stem cells (BMMSCs). n = 5–7 During each assay Krebs Henseleit buffer was supplemented with 5 mM glucose and, as indicated in each graph, either 0.55 mM albumin (BSA group) or 0.55 mM albumin bound to 0.4 mM palmitate and/or 0.4 mM oleate. In addition, the Krebs Henseleit buffer was supplemented with either [U- 14 C]glucose, [1– 14 C]palmitate, [1– 14 C]oleate, or [5– 3 H]glucose for the measurement of glucose oxidation, palmitate oxidation and uptake, oleate oxidation and uptake, or glycolysis, respectively. Since these experiments assessed the acute effect of palmitate and oleate, BMMSCs were maintained in cell culture media used to culture these immediately up to the start of each assay when the media was switched to Krebs Henseleit buffer supplemented with fatty acids. The levels and type of fatty acid BMMSCs were exposed to is indicated on the x-axis of the figures. * Significantly different from all groups. Values are shown as the mean ± SEM.

    Article Snippet: During experiments assessing the chronic effect of fatty acids on BMMSCs this media was also supplemented with 4% fatty acid free bovine serum albumin (BSA) (Equitech-Bio Inc BAH66) or 4% BSA bound to the indicated type and concentration of fatty acid (Palmitate, Sigma P9767; Oleate, Fluka Analytical 60420; Stearate, Sigma S3381).

    Techniques: Cell Culture

    Effect of 24 hr exposure to palmitate and oleate on human BMMSC mitochondrial membrane potential. A) Images of tetramethylrhodamine methyl ester (TMRM) stained bone marrow mesenchymal stem cells (BMMSCs) treated for 24 hr with indicated treatments. B) Relative TMRM levels. BMMSCs were treated for 24 hr with 0.55 mM bovine serum albumin (BSA) alone or palmitate and/or oleate bound to 0.55 mM albumin. TMRM and Hoechst stain were added to the medium to measure mitochondrial membrane potential and stain nuclei, respectively, and images were taken. All fatty acid treated groups were also treated with media supplemented with 0.55 mM albumin in addition to the type and amount of fatty acid indicated in the figures. 4 separate observations. Values are shown as the mean ± SEM. * Significantly different from the BSA group.

    Journal: PLoS ONE

    Article Title: Effect of Fatty Acids on Human Bone Marrow Mesenchymal Stem Cell Energy Metabolism and Survival

    doi: 10.1371/journal.pone.0120257

    Figure Lengend Snippet: Effect of 24 hr exposure to palmitate and oleate on human BMMSC mitochondrial membrane potential. A) Images of tetramethylrhodamine methyl ester (TMRM) stained bone marrow mesenchymal stem cells (BMMSCs) treated for 24 hr with indicated treatments. B) Relative TMRM levels. BMMSCs were treated for 24 hr with 0.55 mM bovine serum albumin (BSA) alone or palmitate and/or oleate bound to 0.55 mM albumin. TMRM and Hoechst stain were added to the medium to measure mitochondrial membrane potential and stain nuclei, respectively, and images were taken. All fatty acid treated groups were also treated with media supplemented with 0.55 mM albumin in addition to the type and amount of fatty acid indicated in the figures. 4 separate observations. Values are shown as the mean ± SEM. * Significantly different from the BSA group.

    Article Snippet: During experiments assessing the chronic effect of fatty acids on BMMSCs this media was also supplemented with 4% fatty acid free bovine serum albumin (BSA) (Equitech-Bio Inc BAH66) or 4% BSA bound to the indicated type and concentration of fatty acid (Palmitate, Sigma P9767; Oleate, Fluka Analytical 60420; Stearate, Sigma S3381).

    Techniques: Staining

    p21-dependent inhibition of LPA-induced cell proliferation by TGFβ. A . TGFβ inhibits LPA-afforded cell proliferation. MDA-MB-231 and Caov-3 cells in 6-well plates were incubated with LPA (10 µM) or vehicle (BSA) in the presence

    Journal: Molecular Cancer Research

    Article Title: Lysophosphatidic acid-induced p21Waf1 expression mediates the cytostatic response of breast and ovarian cancer cells to transforming growth factor beta

    doi: 10.1158/1541-7786.MCR-11-0340

    Figure Lengend Snippet: p21-dependent inhibition of LPA-induced cell proliferation by TGFβ. A . TGFβ inhibits LPA-afforded cell proliferation. MDA-MB-231 and Caov-3 cells in 6-well plates were incubated with LPA (10 µM) or vehicle (BSA) in the presence

    Article Snippet: Prior to use, LPA and S1P were dissolved in PBS containing 0.5% fatty acid-free bovine serum albumin (BSA) obtained from Roche (Indianapolis, IN).

    Techniques: Inhibition, Multiple Displacement Amplification, Incubation