Journal: International Journal of Medical Sciences
Article Title: Quantification of BCR-ABL mRNA in Plasma/Serum of Patients with Chronic Myelogenous Leukemia
Figure Lengend Snippet: Comparison of methods for real-time PCR of cDNA reverse-transcribed from RNA extracted from plasma/serum of patients with CML. Copy number of BCR-ABL mRNA was compared among real-time PCR using LightCycler-FastStart DNA master SYBR Green I reagents, PCR using SYBR Premix ExTaq reagents and PCR using Universal probe method. The graph is a representative data among three independent experiments, the other two of which were performed at different time by using different samples (plasma or serum). The other series of experiments demonstrated the similar results to the representative data shown in the Figure.
Article Snippet: In fluorescence resonance energy transfer hybridization method using FastStart Universal Probe Master (Roche diagnostics) with LightCycler, PCR conditions were 45 cycles of denaturing (95°C for 10 sec) and annealing (60°C for 25 sec).
Techniques: Real-time Polymerase Chain Reaction, SYBR Green Assay, Polymerase Chain Reaction