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    Thermo Fisher fast universal pcr master mix
    WWL70 treatment alleviated inflammatory response in CCI mouse spinal cord. The lumbar spinal cords 7 days post-CCI were dissected, RNA isolated, <t>cDNA</t> synthesized, and gene expression analyzed by <t>qRT-PCR.</t> Increased expression of IL-6 ( a ), IL-1β ( b ), and CCL2 ( c ) were observed in the CCI vehicle group and significantly decreased by WWL70 (10 mg/kg). (* p
    Fast Universal Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1596 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    fast universal pcr master mix - by Bioz Stars, 2020-07
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    Thermo Fisher taqman fast universal pcr master mix
    Results of PPARγ mRNA expression analysis with <t>TaqMan</t> <t>RT-PCR</t> from trophoblastic tissue. PPARγ mRNA expression was significantly downregulated in the miscarriage groups (SM, 15 cases, p = 0.01) and RM, 16 cases, p = 0.004)) compared to the healthy controls (15 cases). This bar graph shows the mean of relative PPARγ expression; therefore, the presentation of error bars is not appropriate.
    Taqman Fast Universal Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7185 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman fast universal pcr master mix/product/Thermo Fisher
    Average 99 stars, based on 7185 article reviews
    Price from $9.99 to $1999.99
    taqman fast universal pcr master mix - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher taqman universal fast pcr master mix
    Results of PPARγ mRNA expression analysis with <t>TaqMan</t> <t>RT-PCR</t> from trophoblastic tissue. PPARγ mRNA expression was significantly downregulated in the miscarriage groups (SM, 15 cases, p = 0.01) and RM, 16 cases, p = 0.004)) compared to the healthy controls (15 cases). This bar graph shows the mean of relative PPARγ expression; therefore, the presentation of error bars is not appropriate.
    Taqman Universal Fast Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 163 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman universal fast pcr master mix/product/Thermo Fisher
    Average 99 stars, based on 163 article reviews
    Price from $9.99 to $1999.99
    taqman universal fast pcr master mix - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    90
    Thermo Fisher taqmantm fast universal pcr master mix
    Results of PPARγ mRNA expression analysis with <t>TaqMan</t> <t>RT-PCR</t> from trophoblastic tissue. PPARγ mRNA expression was significantly downregulated in the miscarriage groups (SM, 15 cases, p = 0.01) and RM, 16 cases, p = 0.004)) compared to the healthy controls (15 cases). This bar graph shows the mean of relative PPARγ expression; therefore, the presentation of error bars is not appropriate.
    Taqmantm Fast Universal Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqmantm fast universal pcr master mix/product/Thermo Fisher
    Average 90 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    taqmantm fast universal pcr master mix - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

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    WWL70 treatment alleviated inflammatory response in CCI mouse spinal cord. The lumbar spinal cords 7 days post-CCI were dissected, RNA isolated, cDNA synthesized, and gene expression analyzed by qRT-PCR. Increased expression of IL-6 ( a ), IL-1β ( b ), and CCL2 ( c ) were observed in the CCI vehicle group and significantly decreased by WWL70 (10 mg/kg). (* p

    Journal: Journal of Neuroinflammation

    Article Title: WWL70 protects against chronic constriction injury-induced neuropathic pain in mice by cannabinoid receptor-independent mechanisms

    doi: 10.1186/s12974-017-1045-9

    Figure Lengend Snippet: WWL70 treatment alleviated inflammatory response in CCI mouse spinal cord. The lumbar spinal cords 7 days post-CCI were dissected, RNA isolated, cDNA synthesized, and gene expression analyzed by qRT-PCR. Increased expression of IL-6 ( a ), IL-1β ( b ), and CCL2 ( c ) were observed in the CCI vehicle group and significantly decreased by WWL70 (10 mg/kg). (* p

    Article Snippet: Fifty nanograms of cDNA was added to the qPCR reaction containing 1× fast universal PCR master mix (Applied Biosystems, Grand Island, NY), and 100 nM of each primer was analyzed in a CFX96™ Real-Time System (Bio-Rad, Hercules, CA).

    Techniques: Isolation, Synthesized, Expressing, Quantitative RT-PCR

    The expression pattern of IFN mRNA and protein differs with each TLR ligand. ( a ) PBMC were stimulated with each TLR ligand and the cells were harvested for qRT-PCR analysis. The geometric means of the peak responses to poly I:C (8 h), LPS (4 h), imiquimod and CpG and unstimulated control (16 h each) from six donors are shown in log 10 scale as a function of expression of the HKG UBC (ΔCq, left), or as copy number μg –1 RNA (right). IFN-α subtypes are ordered according to the phylogenetic plot of amino acid sequence similarity shown in Supplementary Figure 4. ( b – e ) IFN-α, -β and -λ proteins were measured by ELISA, and detectable protein levels were plotted as a function of gene expression. ( b ) The expression of each IFN-α subtype in copy number μg –1 RNA was added, and the sum was plotted against protein expression. ( c ) Correlation of transcript (sum of all subtypes) and protein levels of IFN-α at 8h post-stimulation with poly I:C and CpG oligonucleotides. ( d ) Levels of IFN-β transcript and protein expressed by PBMC at 16 and 24 h in response to CpG oligonucleotides correlate. ( e ) Levels of IFN-λ (sum of IFN-λ1, -λ2 and λ3) transcript and protein expressed by PBMC at 8, 16 and 24 h in response to poly I:C correlate.

    Journal: Immunology and Cell Biology

    Article Title: Expression profiles of human interferon-alpha and interferon-lambda subtypes are ligand- and cell-dependent

    doi: 10.1038/icb.2011.109

    Figure Lengend Snippet: The expression pattern of IFN mRNA and protein differs with each TLR ligand. ( a ) PBMC were stimulated with each TLR ligand and the cells were harvested for qRT-PCR analysis. The geometric means of the peak responses to poly I:C (8 h), LPS (4 h), imiquimod and CpG and unstimulated control (16 h each) from six donors are shown in log 10 scale as a function of expression of the HKG UBC (ΔCq, left), or as copy number μg –1 RNA (right). IFN-α subtypes are ordered according to the phylogenetic plot of amino acid sequence similarity shown in Supplementary Figure 4. ( b – e ) IFN-α, -β and -λ proteins were measured by ELISA, and detectable protein levels were plotted as a function of gene expression. ( b ) The expression of each IFN-α subtype in copy number μg –1 RNA was added, and the sum was plotted against protein expression. ( c ) Correlation of transcript (sum of all subtypes) and protein levels of IFN-α at 8h post-stimulation with poly I:C and CpG oligonucleotides. ( d ) Levels of IFN-β transcript and protein expressed by PBMC at 16 and 24 h in response to CpG oligonucleotides correlate. ( e ) Levels of IFN-λ (sum of IFN-λ1, -λ2 and λ3) transcript and protein expressed by PBMC at 8, 16 and 24 h in response to poly I:C correlate.

    Article Snippet: Primer/probe sets for the HKG and PCR master mix (Universal Fast Mastermix) were purchased from Applied Biosystems (Foster City, CA, USA).

    Techniques: Expressing, Quantitative RT-PCR, Sequencing, Enzyme-linked Immunosorbent Assay

    Results of PPARγ mRNA expression analysis with TaqMan RT-PCR from trophoblastic tissue. PPARγ mRNA expression was significantly downregulated in the miscarriage groups (SM, 15 cases, p = 0.01) and RM, 16 cases, p = 0.004)) compared to the healthy controls (15 cases). This bar graph shows the mean of relative PPARγ expression; therefore, the presentation of error bars is not appropriate.

    Journal: International Journal of Molecular Sciences

    Article Title: PPARγ Expression Is Diminished in Macrophages of Recurrent Miscarriage Placentas

    doi: 10.3390/ijms19071872

    Figure Lengend Snippet: Results of PPARγ mRNA expression analysis with TaqMan RT-PCR from trophoblastic tissue. PPARγ mRNA expression was significantly downregulated in the miscarriage groups (SM, 15 cases, p = 0.01) and RM, 16 cases, p = 0.004)) compared to the healthy controls (15 cases). This bar graph shows the mean of relative PPARγ expression; therefore, the presentation of error bars is not appropriate.

    Article Snippet: Each reaction was accomplished with a volume of 20 µL, including 1 µL cDNA, 8 µL H2 O (DEPC treated DI water; Sigma, Taufkirchen, Germany) and 10 µL TaqMan® Fast Universal PCR Master Mix 2× (Applied Biosystems, Nr.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

    Comparison of miRNA expression levels obtained by NGS Illumina sequencing vs. TaqMan PCR. The results of the correlation are visualized as bar diagrams on the left and scatter diagrams with regression lines on the right comparing next generation sequencing (blue/y-axis) and TaqMan fold changes (red/x-axis) for hsa-miR-378a ( A ), hsa-miR-375 ( B ), hsa-miR-21-5p ( C ) and hsa-miR-215-5p ( D ). There was a good correlation of the NGS with the TaqMan results with a strong relationship for hsa-miR-378a (Pearson’s r = 0.9), hsa-miR-375 ( r = 0.77) and hsa-miR-215-5p ( r = 0.75), but only a moderate relationship for hsa-miR-21-5p ( r = 0.53).

    Journal: Oncotarget

    Article Title: Extensive screening of microRNA populations identifies hsa-miR-375 and hsa-miR-133a-3p as selective markers for human rectal and colon cancer

    doi: 10.18632/oncotarget.25535

    Figure Lengend Snippet: Comparison of miRNA expression levels obtained by NGS Illumina sequencing vs. TaqMan PCR. The results of the correlation are visualized as bar diagrams on the left and scatter diagrams with regression lines on the right comparing next generation sequencing (blue/y-axis) and TaqMan fold changes (red/x-axis) for hsa-miR-378a ( A ), hsa-miR-375 ( B ), hsa-miR-21-5p ( C ) and hsa-miR-215-5p ( D ). There was a good correlation of the NGS with the TaqMan results with a strong relationship for hsa-miR-378a (Pearson’s r = 0.9), hsa-miR-375 ( r = 0.77) and hsa-miR-215-5p ( r = 0.75), but only a moderate relationship for hsa-miR-21-5p ( r = 0.53).

    Article Snippet: Total RNA was transcribed to cDNA using the TaqMan® MicroRNA Reverse Transcription Kit (Applied Biosystems, Catalog # 4366597). cDNAs were amplified using the TaqMan® Fast Universal PCR Master Mix (2X), no AmpErase® UNG (Applied Biosystems, Catalog # 4352042).

    Techniques: Expressing, Next-Generation Sequencing, Sequencing, Polymerase Chain Reaction

    Depletion of CCR2 + cells impairs CD4 + T cell activation and leads to the death of fbp1 Δ mutant yeast-infected mice. CCR2-DTR mice and control (DTR-negative) littermates were treated with 250 ng of DT intraperitoneally before and after infection with 10 6 fbp1 Δ mutant cells as illustrated in panel A. (B) Survival curve of fbp1 Δ mutant yeast - infected, CCR2-depleted mice (dashed red line) and control littermates (solid red line). The data shown are cumulative from three independent experiments with four or five mice per group. ****, P ≤ 0.0001 (determined by log rank [Mantel-Cox] test). (C) Total number of CD4 + T cells recovered from the BALF of fbp1 Δ mutant - infected, CCR2-depleted mice (red symbols) and control littermates (black symbols) on day 6 after infection. Each symbol represents one mouse. The data shown are cumulative from two experiments with four or five mice per group ***, P ≤ 0.001 (determined by Mann-Whitney test). (D) Representative FACS profile of cytokine production by CD4 + T cells recovered from the BALF of fbp1 Δ mutant - infected, CCR2-depleted mice or control littermates. Plots are gated on Thy1.2 + CD4 + CD8 − lymphocytes in BALF. (E) CFU counts in lung tissue of fbp1 Δ mutant - infected, CCR2-depleted mice (red symbols) and control littermates (black symbols) on day 6 after infection. Data are cumulative from two independent experiments with three or four mice per group. ***, P ≤ 0.001 (determined by Mann-Whitney test). (F) Cytokine gene transcription in lung tissue was examined on day 6 after infection with the fbp1 Δ mutant in CCR2-depleted mice (red bars) and control littermates (red striped bars). Control C57BL/6J mice infected with H99 were also analyzed as a control population (black bars). Differential gene expression relative to GAPDH was examined by qRT-PCR with cytokine-specific TaqMan probes and calculated by the ΔΔ CT method. The data shown are cumulative from two independent experiments with four mice per group and are depicted as mean ± the standard error of the mean. **, P ≤ 0.01 (determined by Mann-Whitney test).

    Journal: mBio

    Article Title: The F-Box Protein Fbp1 Shapes the Immunogenic Potential of Cryptococcus neoformans

    doi: 10.1128/mBio.01828-17

    Figure Lengend Snippet: Depletion of CCR2 + cells impairs CD4 + T cell activation and leads to the death of fbp1 Δ mutant yeast-infected mice. CCR2-DTR mice and control (DTR-negative) littermates were treated with 250 ng of DT intraperitoneally before and after infection with 10 6 fbp1 Δ mutant cells as illustrated in panel A. (B) Survival curve of fbp1 Δ mutant yeast - infected, CCR2-depleted mice (dashed red line) and control littermates (solid red line). The data shown are cumulative from three independent experiments with four or five mice per group. ****, P ≤ 0.0001 (determined by log rank [Mantel-Cox] test). (C) Total number of CD4 + T cells recovered from the BALF of fbp1 Δ mutant - infected, CCR2-depleted mice (red symbols) and control littermates (black symbols) on day 6 after infection. Each symbol represents one mouse. The data shown are cumulative from two experiments with four or five mice per group ***, P ≤ 0.001 (determined by Mann-Whitney test). (D) Representative FACS profile of cytokine production by CD4 + T cells recovered from the BALF of fbp1 Δ mutant - infected, CCR2-depleted mice or control littermates. Plots are gated on Thy1.2 + CD4 + CD8 − lymphocytes in BALF. (E) CFU counts in lung tissue of fbp1 Δ mutant - infected, CCR2-depleted mice (red symbols) and control littermates (black symbols) on day 6 after infection. Data are cumulative from two independent experiments with three or four mice per group. ***, P ≤ 0.001 (determined by Mann-Whitney test). (F) Cytokine gene transcription in lung tissue was examined on day 6 after infection with the fbp1 Δ mutant in CCR2-depleted mice (red bars) and control littermates (red striped bars). Control C57BL/6J mice infected with H99 were also analyzed as a control population (black bars). Differential gene expression relative to GAPDH was examined by qRT-PCR with cytokine-specific TaqMan probes and calculated by the ΔΔ CT method. The data shown are cumulative from two independent experiments with four mice per group and are depicted as mean ± the standard error of the mean. **, P ≤ 0.01 (determined by Mann-Whitney test).

    Article Snippet: TaqMan Fast Universal PCR master mix (2×) No Amp and TaqMan probes (Applied Biosystems) for each gene were used and normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH).

    Techniques: Activation Assay, Mutagenesis, Infection, Mouse Assay, MANN-WHITNEY, FACS, Expressing, Quantitative RT-PCR

    Effective maturation of CCR2 + Ly6C + monocytes into mo-DCs after fbp1 Δ mutant yeast infection. Differentiation of mo-DCs was analyzed on day 3 after i.n. infection with H99 (black bars) or the fbp1 Δ mutant (red bars). The data shown are cumulative from two independent experiments with four or five mice per group and are depicted as the mean ± the standard error of the mean. (A) Representative FACS profile of Ly6C + monocyte maturation into mo-DCs (defined as CD45 + CD11b + Ly6C hi Ly6G-CD11c + class II + ) in H99- or fbp1 Δ mutant-infected mice. MHC, major histocompatibility complex. (B) Percentages of mo-DCs (CD11c + class II + ) in monocyte gate (CD45 + CD11b + Ly6C hi Ly6G − ) in mice infected with H99 (black bar) or the fbp1 Δ mutant (red). (C) Total numbers of mo-DCs recruited to the lungs. (D to F) Total numbers of recruited neutrophils (D), monocytes (E), and mo-DCs (F) on day 3 after infection with H99 (black symbols), the fbp1 Δ mutant (red symbols), or the complemented strain ( fbp1 Δ FBP1 ) (gray symbols). (G to I) Chemokine expression in lung tissue was analyzed by qRT-PCR. The expression of each CCR2 ligand was examined with TaqMan probes. Differential gene expression relative to GAPDH was calculated by the ΔΔ CT method. **, P ≤ 0.01 (determined by Mann-Whitney test).

    Journal: mBio

    Article Title: The F-Box Protein Fbp1 Shapes the Immunogenic Potential of Cryptococcus neoformans

    doi: 10.1128/mBio.01828-17

    Figure Lengend Snippet: Effective maturation of CCR2 + Ly6C + monocytes into mo-DCs after fbp1 Δ mutant yeast infection. Differentiation of mo-DCs was analyzed on day 3 after i.n. infection with H99 (black bars) or the fbp1 Δ mutant (red bars). The data shown are cumulative from two independent experiments with four or five mice per group and are depicted as the mean ± the standard error of the mean. (A) Representative FACS profile of Ly6C + monocyte maturation into mo-DCs (defined as CD45 + CD11b + Ly6C hi Ly6G-CD11c + class II + ) in H99- or fbp1 Δ mutant-infected mice. MHC, major histocompatibility complex. (B) Percentages of mo-DCs (CD11c + class II + ) in monocyte gate (CD45 + CD11b + Ly6C hi Ly6G − ) in mice infected with H99 (black bar) or the fbp1 Δ mutant (red). (C) Total numbers of mo-DCs recruited to the lungs. (D to F) Total numbers of recruited neutrophils (D), monocytes (E), and mo-DCs (F) on day 3 after infection with H99 (black symbols), the fbp1 Δ mutant (red symbols), or the complemented strain ( fbp1 Δ FBP1 ) (gray symbols). (G to I) Chemokine expression in lung tissue was analyzed by qRT-PCR. The expression of each CCR2 ligand was examined with TaqMan probes. Differential gene expression relative to GAPDH was calculated by the ΔΔ CT method. **, P ≤ 0.01 (determined by Mann-Whitney test).

    Article Snippet: TaqMan Fast Universal PCR master mix (2×) No Amp and TaqMan probes (Applied Biosystems) for each gene were used and normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH).

    Techniques: Mutagenesis, Infection, Mouse Assay, FACS, Expressing, Quantitative RT-PCR, MANN-WHITNEY

    Interrogating Downstream Effectors of GSK3 Inhibition in Rat ESCs (A) TopFlash assay of β-catenin transcriptional activity in rat and mouse ESCs in PL, T2iL, and 2iL. Values are normalized to mouse ESCs in PL. Error bars represent SD of technical triplicates. (B) Immunostaining of β-catenin in rat ESCs cultured in T2iL and 2iL. (C) qRT-PCR analysis of Tcf1 , Tcf3 , Tcf4 , and Lef1 in rat ESCs maintained in T2iL, using TaqMan probes. Values are normalized to Gapdh and relative to Tcf1 . (D) qRT-PCR analysis using conserved primers of Lef1 expression in rat and mouse ESCs maintained in 2iL. (E) qRT-PCR analysis of a panel of gene expression after Tcf3 and Lef1 knockdown. Gene expression was normalized to Gapdh and relative to values in siGFP transfected cells cultured in 2iL. Error bars represent SD of technical triplicates. (F) Effect of stable knockdown of LEF1 on colony formation in 2iL or T2iL. A total of 80 cells were plated per well and analyzed by AP staining after 5 days. Error bars are SDs of four technical replicates. Data were analyzed by unpaired t test. ∗ p

    Journal: Stem Cell Reports

    Article Title: Robust Self-Renewal of Rat Embryonic Stem Cells Requires Fine-Tuning of Glycogen Synthase Kinase-3 Inhibition

    doi: 10.1016/j.stemcr.2013.07.003

    Figure Lengend Snippet: Interrogating Downstream Effectors of GSK3 Inhibition in Rat ESCs (A) TopFlash assay of β-catenin transcriptional activity in rat and mouse ESCs in PL, T2iL, and 2iL. Values are normalized to mouse ESCs in PL. Error bars represent SD of technical triplicates. (B) Immunostaining of β-catenin in rat ESCs cultured in T2iL and 2iL. (C) qRT-PCR analysis of Tcf1 , Tcf3 , Tcf4 , and Lef1 in rat ESCs maintained in T2iL, using TaqMan probes. Values are normalized to Gapdh and relative to Tcf1 . (D) qRT-PCR analysis using conserved primers of Lef1 expression in rat and mouse ESCs maintained in 2iL. (E) qRT-PCR analysis of a panel of gene expression after Tcf3 and Lef1 knockdown. Gene expression was normalized to Gapdh and relative to values in siGFP transfected cells cultured in 2iL. Error bars represent SD of technical triplicates. (F) Effect of stable knockdown of LEF1 on colony formation in 2iL or T2iL. A total of 80 cells were plated per well and analyzed by AP staining after 5 days. Error bars are SDs of four technical replicates. Data were analyzed by unpaired t test. ∗ p

    Article Snippet: For real-time PCR, we used TaqMan Fast Universal Master Mix and TaqMan probes (Applied Biosystems) or Fast SYBR Green Master Mix and primers ( ).

    Techniques: Inhibition, TOPFlash assay, Activity Assay, Immunostaining, Cell Culture, Quantitative RT-PCR, Expressing, Transfection, Staining

    Inflammatory responses of CCR2 + Mo and Mo-DC during respiratory fungal infection. Lung CCR2 + Mo (GFP + CD45 + CD11b + CD11c − Nk1.1 − ) and Mo-DC (GFP + CD45 + CD11b + CD11c + NK1.1 − ) were FACS sorted 48 h p.i. from CCR2 reporter mice (purity > 97% for all sorts) for transcriptome analysis by RNA-seq (A) or for quantitative RT-PCR (B). Control CCR2 + Mo were also isolated from the lung of uninfected CCR2 reporter mice (naïve sample) to > 97% purity. (A) Gene expression data shown in A is for one experiment and representative of 3 independent biological replicates and three idependent sequencing reactions using SOLiD sequencing platform. Differences in gene expression are shown as fragments per kilobase (FPKM) as calculated using Cufflinks and R software. (B) The graphs show expression of specific transcripts in the indicated cell populations by qRT-PCR using Taq-Man probes normalized to GAPDH. Data shown is mean ±SEM pooled from two separte experiments. (C) The graph shows pulmonary Nos2 induction in DT-treated CCR2 depleter and control mice at the indicated time points p.i. Data shown is mean ±SEM pooled from two separte experiments with 3 mice per group per time point. (D–E) The scatterplots show mean ± SEM lung (D) IL-12p70 and (E) TNF levels at 48 h p.i. in CCR2 depleter (grey circles) and control B6 mice (black circles) as in Figure 3A .

    Journal: PLoS Pathogens

    Article Title: Inflammatory Monocytes Orchestrate Innate Antifungal Immunity in the Lung

    doi: 10.1371/journal.ppat.1003940

    Figure Lengend Snippet: Inflammatory responses of CCR2 + Mo and Mo-DC during respiratory fungal infection. Lung CCR2 + Mo (GFP + CD45 + CD11b + CD11c − Nk1.1 − ) and Mo-DC (GFP + CD45 + CD11b + CD11c + NK1.1 − ) were FACS sorted 48 h p.i. from CCR2 reporter mice (purity > 97% for all sorts) for transcriptome analysis by RNA-seq (A) or for quantitative RT-PCR (B). Control CCR2 + Mo were also isolated from the lung of uninfected CCR2 reporter mice (naïve sample) to > 97% purity. (A) Gene expression data shown in A is for one experiment and representative of 3 independent biological replicates and three idependent sequencing reactions using SOLiD sequencing platform. Differences in gene expression are shown as fragments per kilobase (FPKM) as calculated using Cufflinks and R software. (B) The graphs show expression of specific transcripts in the indicated cell populations by qRT-PCR using Taq-Man probes normalized to GAPDH. Data shown is mean ±SEM pooled from two separte experiments. (C) The graph shows pulmonary Nos2 induction in DT-treated CCR2 depleter and control mice at the indicated time points p.i. Data shown is mean ±SEM pooled from two separte experiments with 3 mice per group per time point. (D–E) The scatterplots show mean ± SEM lung (D) IL-12p70 and (E) TNF levels at 48 h p.i. in CCR2 depleter (grey circles) and control B6 mice (black circles) as in Figure 3A .

    Article Snippet: Taq Man Fast Universal PCR Master Mix (2×) No Amp and TaqMan probes (Applied Biosystems) for each gene were used, and normalized to GAPDH.

    Techniques: Infection, FACS, Mouse Assay, RNA Sequencing Assay, Quantitative RT-PCR, Isolation, Expressing, Sequencing, Software