fast sybr green master mix Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher fast sybr fast green master mix
    HuR associates with SCN5A mRNA (A) Schematic representation of the SCN5A mRNA ARE sites in its 3′-UTR. The length of the mRNA is indicated by a kilo base (kb)-scale. (B) HuR binds to the SCN5A mRNA. After IP of RNA-protein complexes from cardiomyocytes using either anti-HuR antibody (Anti-HuR) or control IgG, RNA was isolated, and then, the cDNA was synthesized in a presence or absence of the reverse transcription enzyme. Two sets of primers to different regions of SCN5A were used to amplify SCN5A fragments. The PCR products were visualized with <t>SYBR</t> ™ Safe DNA Gel Stain in agarose gels. (C) <t>qRT-PCR</t> was performed to confirm the results in (B).
    Fast Sybr Fast Green Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9172 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fast sybr fast green master mix/product/Thermo Fisher
    Average 99 stars, based on 9172 article reviews
    Price from $9.99 to $1999.99
    fast sybr fast green master mix - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    HuR associates with SCN5A mRNA (A) Schematic representation of the SCN5A mRNA ARE sites in its 3′-UTR. The length of the mRNA is indicated by a kilo base (kb)-scale. (B) HuR binds to the SCN5A mRNA. After IP of RNA-protein complexes from cardiomyocytes using either anti-HuR antibody (Anti-HuR) or control IgG, RNA was isolated, and then, the cDNA was synthesized in a presence or absence of the reverse transcription enzyme. Two sets of primers to different regions of SCN5A were used to amplify SCN5A fragments. The PCR products were visualized with SYBR ™ Safe DNA Gel Stain in agarose gels. (C) qRT-PCR was performed to confirm the results in (B).

    Journal: Heart rhythm

    Article Title: HuR-mediated SCN5A mRNA stability reduces arrhythmic risk in heart failure

    doi: 10.1016/j.hrthm.2018.02.018

    Figure Lengend Snippet: HuR associates with SCN5A mRNA (A) Schematic representation of the SCN5A mRNA ARE sites in its 3′-UTR. The length of the mRNA is indicated by a kilo base (kb)-scale. (B) HuR binds to the SCN5A mRNA. After IP of RNA-protein complexes from cardiomyocytes using either anti-HuR antibody (Anti-HuR) or control IgG, RNA was isolated, and then, the cDNA was synthesized in a presence or absence of the reverse transcription enzyme. Two sets of primers to different regions of SCN5A were used to amplify SCN5A fragments. The PCR products were visualized with SYBR ™ Safe DNA Gel Stain in agarose gels. (C) qRT-PCR was performed to confirm the results in (B).

    Article Snippet: Quantitative real-time reverse-transcriptase polymerase chain reaction (qRT-PCR) was carried out using gene-specific primers, Fast SYBR® Green Master Mix and 7500 Fast Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA).

    Techniques: Isolation, Synthesized, Polymerase Chain Reaction, Staining, Quantitative RT-PCR

    Genotypic levels of total RpL 14 mRNA expression in groups of whole individuals. Amount of RpL 14 mRNA in adults and larvae relative to three normalization genes. Height of bars indicate total amount of RpL 14 mRNA based on SYBR green-based quantitative reverse-transcription PCR and each bar is split to represent the proportion of total RpL 14 expressed from the RpL 14 [r] gene in { Ud }86 (white) and the endogenous RpL 14 [+] gene (gray), based on gene-specific TaqMan probes. Error bars represent 1 standard error for three biological replicates.

    Journal: PLoS ONE

    Article Title: First Steps towards Underdominant Genetic Transformation of Insect Populations

    doi: 10.1371/journal.pone.0097557

    Figure Lengend Snippet: Genotypic levels of total RpL 14 mRNA expression in groups of whole individuals. Amount of RpL 14 mRNA in adults and larvae relative to three normalization genes. Height of bars indicate total amount of RpL 14 mRNA based on SYBR green-based quantitative reverse-transcription PCR and each bar is split to represent the proportion of total RpL 14 expressed from the RpL 14 [r] gene in { Ud }86 (white) and the endogenous RpL 14 [+] gene (gray), based on gene-specific TaqMan probes. Error bars represent 1 standard error for three biological replicates.

    Article Snippet: 1 µL of the resulting reaction was used as a template for qPCR, using TaqMan Fast Master Mix (Applied Biosystems) for determining the relative ratios of wild-type and rescue transcripts or SYBR Green Fast Master Mix (Applied Biosystems) for total RpL14 mRNA levels.

    Techniques: Expressing, SYBR Green Assay, Polymerase Chain Reaction

    The BAC NEUROG3-SeAP/EGFP transgenes are expressed in the developing pancreas. (A) Homologous recombination ( Yang et al., 1997 ) was used to replace the human neurogenin-3 coding sequence in the NEUROG3 BAC (RP11-343J3T) with two reporter genes, SeAP and EGFP, flanked on the 5′ end by the human β-globulin intron and the 3′ end by the SV40 polyadenylation signal, and separated by a viral IRES. (B) Levels of neurogenin-3 mRNA in mouse pancreas were measured by real-time TaqMan RT-PCR at the embryonic dates shown and in adult islets, and are expressed relative to levels of histone H3.3a mRNA. (C) Levels of the SeAP / EGF P mRNA in mouse pancreas were measured by real-time SYBR Green RT-PCR at the embryonic dates shown and are expressed relative to levels of mouse β-actin mRNA. (D) Tissue SeAP activity was measured in pancreas homogenates at the embryonic dates shown and is expressed relative to total protein. All data represent mean + s.e.m. from at least three independent experiments.

    Journal: Disease Models & Mechanisms

    Article Title: A mouse model for monitoring islet cell genesis and developing therapies for diabetes

    doi: 10.1242/dmm.002998

    Figure Lengend Snippet: The BAC NEUROG3-SeAP/EGFP transgenes are expressed in the developing pancreas. (A) Homologous recombination ( Yang et al., 1997 ) was used to replace the human neurogenin-3 coding sequence in the NEUROG3 BAC (RP11-343J3T) with two reporter genes, SeAP and EGFP, flanked on the 5′ end by the human β-globulin intron and the 3′ end by the SV40 polyadenylation signal, and separated by a viral IRES. (B) Levels of neurogenin-3 mRNA in mouse pancreas were measured by real-time TaqMan RT-PCR at the embryonic dates shown and in adult islets, and are expressed relative to levels of histone H3.3a mRNA. (C) Levels of the SeAP / EGF P mRNA in mouse pancreas were measured by real-time SYBR Green RT-PCR at the embryonic dates shown and are expressed relative to levels of mouse β-actin mRNA. (D) Tissue SeAP activity was measured in pancreas homogenates at the embryonic dates shown and is expressed relative to total protein. All data represent mean + s.e.m. from at least three independent experiments.

    Article Snippet: Quantification of SeAP /EGFP cDNA was performed with SYBR Green using Fast SYBR Master Mix (Applied Biosystems) and reported relative to levels of the cDNA encoding mouse β-actin.

    Techniques: BAC Assay, Homologous Recombination, Sequencing, Reverse Transcription Polymerase Chain Reaction, SYBR Green Assay, Activity Assay

    Assessment of BRCA1 loss (A) Mutation screening showing the abnormal denaturing high performance liquid chromatography profile corresponding to the 1351delAT mutation in tumor 223. The single blue line represents the electropherogram from a normal control, while the purple line represents the abnormal profile formed by the mutated exon 11c in tumor 223. (B) Direct DNA sequencing demonstrating the 185delAG mutation in tumor 283. Only the mutant allele is seen in the tumor because LOH is present. (C-E) Loss of heterozygosity (LOH) analysis using BRCA1-associated microsatellite markers visualized on an ABI Prism 3100 Genetic Analyzer, where LOH is defined as > 50% decrease in area under the curve when germline DNA (upper tracing) and tumor DNA (lower tracing) are compared. (C) The lack of LOH in tumor 240 demonstrated using microsatellite marker D17S1185, (D) LOH in tumor 283 demonstrated using microsatellite marker D17S855. (E) Microsatellite instability demonstrated in tumor 156 using microsatellite marker D17S1185. (F, G, H, and I) Methylation analysis of BRCA1 gene using fluorescence-based, quantitative, real-time PCR (TaqMan) using SYBR Green 1 as detection method. Two sets of primers, designed specifically for bisulfite converted DNA, were used: a methylated set for the BRCA1 gene and a reference set (MYOD1) to control for input DNA. Specificity of the reactions for methylated DNA were confirmed separately using human genomic DNA (unmethyated; F) and CpG methylated Jurkat genomic DNA (methylated; G), respectively. H and I show representative examples of results from assessment of BRCA1 loss through promoter hypermethyation. Tumor 178 shows only unmethylated BRCA1 promoter, while tumor 345 shows evidence of BRCA1 promoter hypermethylation.

    Journal: BMC Cancer

    Article Title: Ovarian carcinomas with genetic and epigenetic BRCA1 loss have distinct molecular abnormalities

    doi: 10.1186/1471-2407-8-17

    Figure Lengend Snippet: Assessment of BRCA1 loss (A) Mutation screening showing the abnormal denaturing high performance liquid chromatography profile corresponding to the 1351delAT mutation in tumor 223. The single blue line represents the electropherogram from a normal control, while the purple line represents the abnormal profile formed by the mutated exon 11c in tumor 223. (B) Direct DNA sequencing demonstrating the 185delAG mutation in tumor 283. Only the mutant allele is seen in the tumor because LOH is present. (C-E) Loss of heterozygosity (LOH) analysis using BRCA1-associated microsatellite markers visualized on an ABI Prism 3100 Genetic Analyzer, where LOH is defined as > 50% decrease in area under the curve when germline DNA (upper tracing) and tumor DNA (lower tracing) are compared. (C) The lack of LOH in tumor 240 demonstrated using microsatellite marker D17S1185, (D) LOH in tumor 283 demonstrated using microsatellite marker D17S855. (E) Microsatellite instability demonstrated in tumor 156 using microsatellite marker D17S1185. (F, G, H, and I) Methylation analysis of BRCA1 gene using fluorescence-based, quantitative, real-time PCR (TaqMan) using SYBR Green 1 as detection method. Two sets of primers, designed specifically for bisulfite converted DNA, were used: a methylated set for the BRCA1 gene and a reference set (MYOD1) to control for input DNA. Specificity of the reactions for methylated DNA were confirmed separately using human genomic DNA (unmethyated; F) and CpG methylated Jurkat genomic DNA (methylated; G), respectively. H and I show representative examples of results from assessment of BRCA1 loss through promoter hypermethyation. Tumor 178 shows only unmethylated BRCA1 promoter, while tumor 345 shows evidence of BRCA1 promoter hypermethylation.

    Article Snippet: Samples (10 ng bisulfite-treated DNA) were run in triplicate containing 5 μL SYBR Green Master Mix (Applied Biosystems, Foster City, CA) and 5 pmol of each forward and reverse primer.

    Techniques: Mutagenesis, High Performance Liquid Chromatography, DNA Sequencing, Marker, Methylation, Fluorescence, Real-time Polymerase Chain Reaction, SYBR Green Assay

    Src homology 2 domain–containing adaptor protein B (SHB) expression in bone marrow (BM)–derived dendritic cell (BMDC) development. BM cells from C57BL/6 mice were cultured for 6 days in the presence of granulocyte-macrophage colony-stimulating factor (10 ng/mL) to generate BMDCs. (A) Total RNA was isolated from immature dendritic cells (imDCs) and lipopolysaccharide (LPS)-treated mature dendritic cells (mDCs), and SHB mRNA was assessed from each sample by reverse transcriptase polymerase chain reaction (RT-PCR) and quantitative real-time polymerase chain reaction (qRT-PCR) with the Maxime RT-PCR PreMix (iNtRON) and Fast SYBR Green Master Mix (Life Technologies) kits, respectively. (B) The amount of SHB protein expressed in imDCs and mDCs was assessed by Western blot (WB) assay. (C) SHB expression in imDCs and mDCs was assessed by fluorescence-activated cell sorting after intracellular staining. (D, E) Splenic DCs (spDCs) were isolated from mice using a CD11c + isolation kit (Miltenyi Biotech) and treated or not with LPS (200 ng/mL) for 24 hours. Intracellular SHB protein expression in spDCs was assessed by fluorescence-activated cell sorter (D) and WB assay (E). RT-PCR data are shown as the mean±standard deviation of nine samples pooled from three independent experiments.

    Journal: Clinical and Experimental Vaccine Research

    Article Title: SH2 domain–containing adaptor protein B expressed in dendritic cells is involved in T-cell homeostasis by regulating dendritic cell–mediated Th2 immunity

    doi: 10.7774/cevr.2017.6.1.50

    Figure Lengend Snippet: Src homology 2 domain–containing adaptor protein B (SHB) expression in bone marrow (BM)–derived dendritic cell (BMDC) development. BM cells from C57BL/6 mice were cultured for 6 days in the presence of granulocyte-macrophage colony-stimulating factor (10 ng/mL) to generate BMDCs. (A) Total RNA was isolated from immature dendritic cells (imDCs) and lipopolysaccharide (LPS)-treated mature dendritic cells (mDCs), and SHB mRNA was assessed from each sample by reverse transcriptase polymerase chain reaction (RT-PCR) and quantitative real-time polymerase chain reaction (qRT-PCR) with the Maxime RT-PCR PreMix (iNtRON) and Fast SYBR Green Master Mix (Life Technologies) kits, respectively. (B) The amount of SHB protein expressed in imDCs and mDCs was assessed by Western blot (WB) assay. (C) SHB expression in imDCs and mDCs was assessed by fluorescence-activated cell sorting after intracellular staining. (D, E) Splenic DCs (spDCs) were isolated from mice using a CD11c + isolation kit (Miltenyi Biotech) and treated or not with LPS (200 ng/mL) for 24 hours. Intracellular SHB protein expression in spDCs was assessed by fluorescence-activated cell sorter (D) and WB assay (E). RT-PCR data are shown as the mean±standard deviation of nine samples pooled from three independent experiments.

    Article Snippet: Quantitative PCR was performed using the Fast SYBR Green Master Mix kit (Life Technologies).

    Techniques: Expressing, Derivative Assay, Mouse Assay, Cell Culture, Isolation, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, SYBR Green Assay, Western Blot, Fluorescence, FACS, Staining, Standard Deviation

    Increased PCR sensitivity for CC75-08 in the hydrolysis probe PCR. Analytical sensitivity equal for S . aureus (CCUG31966) in hydrolysis probe PCR and SybrGreen PCR. Analytical sensitivity increased for CC75 lineage/ S . argenteus (CC75-08) strains in hydrolysis probe PCR.

    Journal: PLoS ONE

    Article Title: Introduction of a hydrolysis probe PCR assay for high-throughput screening of methicillin-resistant Staphylococcus aureus with the ability to include or exclude detection of Staphylococcus argenteus

    doi: 10.1371/journal.pone.0192782

    Figure Lengend Snippet: Increased PCR sensitivity for CC75-08 in the hydrolysis probe PCR. Analytical sensitivity equal for S . aureus (CCUG31966) in hydrolysis probe PCR and SybrGreen PCR. Analytical sensitivity increased for CC75 lineage/ S . argenteus (CC75-08) strains in hydrolysis probe PCR.

    Article Snippet: Primary detection amplification conditions Amplification of the nuc gene using SybrGreen was carried out in a 20 μL reaction mix using 2x Fast SybrGreen Master Mix (ThermoFisher), 0,5 μM of each nuc -SG primer (Eurogentec, Seraing, Belgium) and 5 μL of DNA template.

    Techniques: Polymerase Chain Reaction

    Increased stability using hydrolysis probe PCR. Cq values from PCR control collected during one year from hydrolysis probe PCR (a) and SybrGreen PCR (b) show increased stability in hydrolysis probe PCR.

    Journal: PLoS ONE

    Article Title: Introduction of a hydrolysis probe PCR assay for high-throughput screening of methicillin-resistant Staphylococcus aureus with the ability to include or exclude detection of Staphylococcus argenteus

    doi: 10.1371/journal.pone.0192782

    Figure Lengend Snippet: Increased stability using hydrolysis probe PCR. Cq values from PCR control collected during one year from hydrolysis probe PCR (a) and SybrGreen PCR (b) show increased stability in hydrolysis probe PCR.

    Article Snippet: Primary detection amplification conditions Amplification of the nuc gene using SybrGreen was carried out in a 20 μL reaction mix using 2x Fast SybrGreen Master Mix (ThermoFisher), 0,5 μM of each nuc -SG primer (Eurogentec, Seraing, Belgium) and 5 μL of DNA template.

    Techniques: Polymerase Chain Reaction