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All (2)
Becton Dickinson
MilliporeSigma
 

About 17 products / 158 snippets / 1 images

91 / 100
Bioz Stars
FACSFlow buffer
Becton Dickinson  
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Techniques
 
Article Snippets
"The sheath fluid consisted of 0.5 × FACSFlow buffer (BD)."  (see more)
90 / 100
Bioz Stars
FACSFlow buffer
Becton Dickinson  
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Techniques
 
Article Snippets
".. and re-suspended at a concentration of 107/mL in BD FACSFlow buffer (BD Biosciences, Cat. No. 324003) supplemented with 1% fetal bovine serum."  (see more)
( A ) Gating strategy of a full stained sample in PBS+BSA buffer unmixed with single stain cells controls in PBS+BSA buffer. The sorted population gate is marked in a blue square with an ultimate marker phenotype of live/CD14-/CD56+/CD7+/CD16+/KLRG-. Cells were sorted into BD <t>FACSFlow™</t> (no added protein) and re-acquired on the BD FACSDiscover™ S8 where a population shift can be observed in the CD57+/KLRG1-sorting gate (blue square, far right plot). ( B ) CD16 BUV563 Single stain cells resuspended in PBS+BSA buffer and BD FACSFlow™ on the BD FACSDiscover™ S8. ( C ) Spectral signature overlays of CD16 BUV563 Single Stain Cells resuspended in PBS+BSA buffer (blue line) and BD FACSFlow™ (red line) on the BD FACS Discover™ S8. ( D ) CD16 BUV563 Single Stain Cells resuspended in Biolegend Staining Buffer (contains BSA) and BD FACSFlow™ on Cytek Aurora Analyser. (E) Spectral signature overlays of CD16 BUV563 Single Stain Cells resuspended in Staining Buffer (blue line) and BD FACSFlow™ (red line) on Cytek Aurora Analyser. ( F ) Full stained sample in PBS+BSA, washed and resuspended in BD FACSFlow™ and washed and resuspended into PBS+BSA and acquired on the BD FACSDiscover S8 (these samples were not sorted only acquired; blue box indicates population of interest) ( G ) Full stained sample resuspended in Biolegend staining buffer, washed, and resuspended in BD FACSFlow™ and acquired on the Aurora Analyser (blue box indicates population of interest). ( H ) Full stained sample resuspended in Biolegend staining buffer, washed, and resuspended in BD FACSFlow™ and acquired on the Aurora CS (these samples were not sorted only acquired, blue box indicates population of interest). All cells used in this figure were unfixed apart from where the cells were fixed with Biolegend Fixation buffer due to the biosafety regulations.
STAIN
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80 / 100
Bioz Stars
FACSFlow buffer
Becton Dickinson  
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Techniques
 
Article Snippets
"Samples were diluted 1:80 into FACSFlow buffer (catalog no. 340398; Becton Dickinson, Franklin Lakes, N.J.) and stored in the dark at room temperature .."  (see more)
85 / 100
Bioz Stars
FACSFlow buffer
Becton Dickinson  
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Techniques
 
Article Snippets
"After washing with 2 ml FACSFlow buffer (sheath fluid, BD 41 Biosciences), the cells were resuspended in 0.5 ml FACSFlow buffer."  (see more)
85 / 100
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FACSFlow sheath buffer
Becton Dickinson / BD Biosciences  
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Techniques
 
Article Snippets
"Cells were fixed with 1.6% paraformaldehyde (Sigma-Aldrich), washed with FACSFlow sheath buffer (BD Biosciences), permeabilized with 100% cold methanol (Fisher Scientific), washed with FACS staining buffer .."  (see more)
91 / 100
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BD FACSFlow buffer
Becton Dickinson / BD Biosciences  
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Techniques
 
Article Snippets
".. a 4◦C centrifuge at 370 × g for 2 min. After washing with cold BD FACSFlow buffer (BD Biosciences, 342003) once, they were incubated with the HA antibody (1:200 HA.11 BAbCO, Clone: 16B12) for .."  (see more)
80 / 100
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FACSFlow™ Stain Buffer
Becton Dickinson / BD Biosciences  
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Techniques
 
Article Snippets
"Cells were resuspended and centrifuged at 500 g for 5 minutes at 40° C. Media was aspirated and cells were resuspended in BD FACSFlowTM Stain Buffer (BD Biosciences, San Jose, CA) with 1% fetal bovine serum (FBS) (cell staining buffer) at 40° C. Cells were centrifuged .."  (see more)
80 / 100
Bioz Stars
FACSFlow staining buffer
Becton Dickinson  
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Techniques
 
Article Snippets
".. PE-Gr1 (all from Biolegend, CA, USA) and incubated for 30 minutes at 4°C in FACSFlow staining buffer (Becton Dickinson)."  (see more)
88 / 100
Bioz Stars
FACSFlow sheath fluid buffer
Becton Dickinson  
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Techniques
 
Article Snippets
"For FC analyses, PKH67-stained culture samples were diluted to an OD 600 of 0.05 in FACSFlow sheath fluid buffer (BD, Heidelberg, Germany)."  (see more)
80 / 100
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X FACSFlow 74 buffer
Becton Dickinson  
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Techniques
 
Article Snippets
"The sheath fluid consisted of 0.5 x FACSFlow 74 buffer (BD)."  (see more)
80 / 100
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Propidium iodide buffer Facsflow
Becton Dickinson  
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Techniques
 
Article Snippets
"Cells were harvested by trypsinization, fixed in 70% ethanol, and resuspended in propidium iodide buffer Facsflow (BD)."  (see more)
80 / 100
Bioz Stars
FACSFlow phosphate-buffered saline solution
Becton Dickinson / BD Biosciences  
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Techniques
 
Article Snippets
"The sheath fluid was FACSFlow phosphate-buffered saline solution (BD Biosciences)."  (see more)