facscalibur Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Becton Dickinson facscalibur flow cytometer
    Dasatinib blocks antigen-induced TCR downregulation and tetramer internalization from the cell surface. A. Mel13 CTL were pre-treated with PBS ± 50 nM dasatinib and exposed to C1R-A2 B cells previously pulsed with 10 -6 M ELAGIGILTV peptide or medium alone for 4 h at 37 °C. Cells were subsequently stained with anti-TCR-FITC (clone BMA 031; Serotec) and anti-CD8-APC (clone RPA-T8; BD Pharmingen) mAbs for 30 min on ice, washed twice and resuspended in PBS. Data were acquired on a <t>FACSCalibur</t> flow cytometer (BD) and analyzed using FlowJo software. B. 10 5 ILA1 CTL were pre-treated with PBS (i ii) or PBS + 50 nM dasatinib (iii iv) for 30 min at 37 °C, then stained with 20μg/ml HLA A2/ILAKFLHWL-Alexa488 tetramer for 15 min at 37 °C. Microscopy was performed as described in the Materials and methods.
    Facscalibur Flow Cytometer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/facscalibur flow cytometer/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    facscalibur flow cytometer - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Becton Dickinson facscalibur
    Non-cognate A2/K b -mediated CTL activation and tetramer binding is not influenced by MHCI restriction A. 2.5×10 5 PBMC were suspended in 250μl FACS buffer (2% FCS/PBS) and stained with FITC-conjugated anti-A2 and 7-AAD for 30 minutes on ice, then washed twice and resuspended in PBS. For pMHCI tetramer staining experiments, 2.5×10 5 PBMC were suspended in 50μl FACS buffer (2% FCS/PBS) and incubated +/− 10μg/ml unconjugated anti-CD8 for 20 minutes on ice, then stained with 10μg/ml of the PE-conjugated tetramers A2 ILAKFLHWL or A2/K b ILAKFLHWL for 45 minutes on ice. After washing, cells were subsequently stained with APC-conjugated anti-CD8 and 7-AAD, washed again and resuspended in PBS. Data were acquired using a <t>FACSCalibur</t> flow cytometer and analyzed with FlowJo software. B. 2.5×10 4 CTL were incubated for 12 hours at 37°C with 10 5 unpulsed C1R cells expressing either A2 or A2/K b on the cell surface. The following CTL clones were used: (i) the HLA A*6801-restricted CTL clone c23, specific for the HIV-1 Tat-derived epitope ITKGLGISYGR (residues 38-48); (ii) the HLA B*0702-restricted CTL clone KD4, specific for the EBV EBNA3A-derived epitope RPPIFIRRL (residues 379-387); (iii) the HLA B*0801-restricted CTL clone LC13, specific for the EBV EBNA3A-derived epitope FLRGRAYGL (residues 339-347); and, (iv) the HLA B*3508-restricted CTL clone SB27, specific for the EBV BZLF1-derived epitope LPEPLPQGQLTAY (residues 52-64). Supernatant was subsequently assayed for MIP-1β content by ELISA. The mean ± SD of two replicate assays is shown.
    Facscalibur, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/facscalibur/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    facscalibur - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Becton Dickinson facscalibur cytometer
    Analysis of transfection efficiency and correlation between transfection efficiency and vector size. The five constructed vectors were transfected into CHO cells using Lipofectamine ® 3000 Transfection Reagent. The transfection efficiency was analysed using a <t>FACSCalibur</t> cytometer. ( A ) Analysis of the transfection efficiency. Three independent experiments were performed in this study. Standard error of the mean (S.E.M.) is indicated. ( B ) Correlation between transfection efficiency and vector size. Transfection efficiency analysed by FACSCalibur cytometer decreased exponentially as vector size increased. The graph was created with Microsoft Office Excel.
    Facscalibur Cytometer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/facscalibur cytometer/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    facscalibur cytometer - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Becton Dickinson bd facscalibur
    Passing and Bablok regression between the BD FACSPresto™ with capillary blood (a-c) and venous blood (d-f) compared to the BD <t>FACSCalibur™.</t> Comparison of the BD FACSPresto™ with BD FACSCalibur™ for CD4+ T-cell count with capillary blood (a) and with venous blood samples (d). Comparison of the BD FACSPresto™ with BD FACSCalibur™ for CD4% with capillary blood (b) and with venous blood samples (e). Comparison of the BD FACSPresto™ with Sysmex XT-1800i™with capillary blood (c) and with venous blood (f).
    Bd Facscalibur, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bd facscalibur/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bd facscalibur - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier




    Image Search Results


    Dasatinib blocks antigen-induced TCR downregulation and tetramer internalization from the cell surface. A. Mel13 CTL were pre-treated with PBS ± 50 nM dasatinib and exposed to C1R-A2 B cells previously pulsed with 10 -6 M ELAGIGILTV peptide or medium alone for 4 h at 37 °C. Cells were subsequently stained with anti-TCR-FITC (clone BMA 031; Serotec) and anti-CD8-APC (clone RPA-T8; BD Pharmingen) mAbs for 30 min on ice, washed twice and resuspended in PBS. Data were acquired on a FACSCalibur flow cytometer (BD) and analyzed using FlowJo software. B. 10 5 ILA1 CTL were pre-treated with PBS (i ii) or PBS + 50 nM dasatinib (iii iv) for 30 min at 37 °C, then stained with 20μg/ml HLA A2/ILAKFLHWL-Alexa488 tetramer for 15 min at 37 °C. Microscopy was performed as described in the Materials and methods.

    Journal: Journal of Immunological Methods

    Article Title: Protein kinase inhibitors substantially improve the physical detection of T-cells with peptide-MHC tetramers

    doi: 10.1016/j.jim.2008.09.014

    Figure Lengend Snippet: Dasatinib blocks antigen-induced TCR downregulation and tetramer internalization from the cell surface. A. Mel13 CTL were pre-treated with PBS ± 50 nM dasatinib and exposed to C1R-A2 B cells previously pulsed with 10 -6 M ELAGIGILTV peptide or medium alone for 4 h at 37 °C. Cells were subsequently stained with anti-TCR-FITC (clone BMA 031; Serotec) and anti-CD8-APC (clone RPA-T8; BD Pharmingen) mAbs for 30 min on ice, washed twice and resuspended in PBS. Data were acquired on a FACSCalibur flow cytometer (BD) and analyzed using FlowJo software. B. 10 5 ILA1 CTL were pre-treated with PBS (i ii) or PBS + 50 nM dasatinib (iii iv) for 30 min at 37 °C, then stained with 20μg/ml HLA A2/ILAKFLHWL-Alexa488 tetramer for 15 min at 37 °C. Microscopy was performed as described in the Materials and methods.

    Article Snippet: The cells were evaluated using a FACSCalibur flow cytometer (BD Biosciences).

    Techniques: CTL Assay, Staining, Recombinase Polymerase Amplification, Flow Cytometry, Cytometry, Software, Microscopy

    Dasatinib results in a time dependent increase in TCR and CD8 expression levels at the CTL cell surface. The ILA1 CTL clone was treated with PBS ± 50 nM dasatinib at 37 °C and 10 5 CTL were removed from the medium at 0, 10, 30, 60, 180 and 250 min. CTL were subsequently stained with anti-CD8 FITC (clone SK1; BD, Pharmingen; left panel) or anti-TCR FITC (clone BMA 031; Serotec; right panel) for 30 min on ice, washed twice and resuspended in PBS. Data were acquired on a FACSCalibur flow cytometer (BD) and analyzed using FlowJo software.

    Journal: Journal of Immunological Methods

    Article Title: Protein kinase inhibitors substantially improve the physical detection of T-cells with peptide-MHC tetramers

    doi: 10.1016/j.jim.2008.09.014

    Figure Lengend Snippet: Dasatinib results in a time dependent increase in TCR and CD8 expression levels at the CTL cell surface. The ILA1 CTL clone was treated with PBS ± 50 nM dasatinib at 37 °C and 10 5 CTL were removed from the medium at 0, 10, 30, 60, 180 and 250 min. CTL were subsequently stained with anti-CD8 FITC (clone SK1; BD, Pharmingen; left panel) or anti-TCR FITC (clone BMA 031; Serotec; right panel) for 30 min on ice, washed twice and resuspended in PBS. Data were acquired on a FACSCalibur flow cytometer (BD) and analyzed using FlowJo software.

    Article Snippet: The cells were evaluated using a FACSCalibur flow cytometer (BD Biosciences).

    Techniques: Expressing, CTL Assay, Staining, Flow Cytometry, Cytometry, Software

    Beneficial effects of dasatinib are not CD8-mediated. A. Melc5 CTL were pre- treated with PBS ± 50 nM dasatinib for 30 min at 37 °C, then stained with HLA A2 DT227/8KA cognate tetramer for 20 min at 37 °C. After washing twice, data were acquired on a FACSCalibur flow cytometer (BD) and analyzed using FlowJo software. B. Staining of HLA A2-restricted CTL lines expanded from PBMC by one round of stimulation with the Melan-A/Mart-1 26-35 peptide (ELAGIGILTV). Lines were stained with either wild type or CD8 null cognate tetramer ± pre-treatment with 50 nM dasatinib for 30 min at 37 °C.

    Journal: Journal of Immunological Methods

    Article Title: Protein kinase inhibitors substantially improve the physical detection of T-cells with peptide-MHC tetramers

    doi: 10.1016/j.jim.2008.09.014

    Figure Lengend Snippet: Beneficial effects of dasatinib are not CD8-mediated. A. Melc5 CTL were pre- treated with PBS ± 50 nM dasatinib for 30 min at 37 °C, then stained with HLA A2 DT227/8KA cognate tetramer for 20 min at 37 °C. After washing twice, data were acquired on a FACSCalibur flow cytometer (BD) and analyzed using FlowJo software. B. Staining of HLA A2-restricted CTL lines expanded from PBMC by one round of stimulation with the Melan-A/Mart-1 26-35 peptide (ELAGIGILTV). Lines were stained with either wild type or CD8 null cognate tetramer ± pre-treatment with 50 nM dasatinib for 30 min at 37 °C.

    Article Snippet: The cells were evaluated using a FACSCalibur flow cytometer (BD Biosciences).

    Techniques: CTL Assay, Staining, Flow Cytometry, Cytometry, Software

    Dasatinib substantially improves pMHC tetramer staining intensity. A. 10 5 ILA1 CTL were re-suspended in 40μl of PBS ± 50 nM dasatinib or Lck inhibitor II (Calbiochem), then incubated at 37 °C for 30 min. Cells were then stained with cognate HLA A2/ILAKFLHWL-PE tetramer at a final concentration of 10µg/ml for 20 min at 37 °C, washed twice in PBS and analyzed on a FACSCalibur (BD) flow cytometer. A > 10-fold increase in median fluorescence intensity (MFI) was observed after treatment with 50 nM dasatinib (blue) or LcK inhibitor II (red) compared to staining without PKI pre-treatment (green line). B. 10 5 ILA1 CTL were treated with various concentrations of dasatinib for 30 min at 37 °C, then stained with either HLA A2/ILAKFLHWL tetramer or the non-cognate HLA A2/ELAGIGILTV tetramer for 20 min at 37 °C before washing with PBS. C. 10 5 ILA1 CTL were resuspended in 40μl of PBS ± the indicated concentration of dasatinib and incubated for 60 min at 37 °C. Cells were then stained with cognate HLA A2/ILAKFLHWL-PE tetramer at a final concentration of 10 μg/ml for 20 min at 37 °C and washed twice in PBS prior to flow cytometric analysis. D. As (A), but ILA1 CTL were incubated with 50 nM dasatinib for various times prior to staining with pMHCI tetramer. For this experiment the drug was washed off prior to staining. E. As (A), but tetramer concentration was varied to stain CTL pre-treated ± 50 nM dasatinib for 30 min. F. 10 5 Mel13 CTL were stained with various concentrations of HLA A2/ELAGIGILTV tetramer following incubation ± 50 nM dasatinib for 30 min. G. 5x10 5 splenocytes from an F5 TCR transgenic Rag + mouse were resuspended in PBS ± 50 nM dasatinib and incubated for 30 min at 37 °C. Cells were subsequently stained with H2-D b /ASNENMDAM-PE tetramer for 20 min at 37 °C followed by anti-CD8 Cy5.5 for 30 min on ice prior to two washes in PBS and analysis by flow cytometry. H. 10 5 cells of the HLA DR⁎0101-restricted, influenza virus A HA 307-319 PKYVKQNTLKLAT-specific CD4 + clone C6 were incubated with PBS ± 50 nM dasatinib for 30 min at 37 °C, then stained with cognate PE-conjugated tetramer for 20 min at 37 °C. Samples were washed with PBS before flow cytometric analysis. Irrelevant tetramer was used as a negative control in all cases.

    Journal: Journal of Immunological Methods

    Article Title: Protein kinase inhibitors substantially improve the physical detection of T-cells with peptide-MHC tetramers

    doi: 10.1016/j.jim.2008.09.014

    Figure Lengend Snippet: Dasatinib substantially improves pMHC tetramer staining intensity. A. 10 5 ILA1 CTL were re-suspended in 40μl of PBS ± 50 nM dasatinib or Lck inhibitor II (Calbiochem), then incubated at 37 °C for 30 min. Cells were then stained with cognate HLA A2/ILAKFLHWL-PE tetramer at a final concentration of 10µg/ml for 20 min at 37 °C, washed twice in PBS and analyzed on a FACSCalibur (BD) flow cytometer. A > 10-fold increase in median fluorescence intensity (MFI) was observed after treatment with 50 nM dasatinib (blue) or LcK inhibitor II (red) compared to staining without PKI pre-treatment (green line). B. 10 5 ILA1 CTL were treated with various concentrations of dasatinib for 30 min at 37 °C, then stained with either HLA A2/ILAKFLHWL tetramer or the non-cognate HLA A2/ELAGIGILTV tetramer for 20 min at 37 °C before washing with PBS. C. 10 5 ILA1 CTL were resuspended in 40μl of PBS ± the indicated concentration of dasatinib and incubated for 60 min at 37 °C. Cells were then stained with cognate HLA A2/ILAKFLHWL-PE tetramer at a final concentration of 10 μg/ml for 20 min at 37 °C and washed twice in PBS prior to flow cytometric analysis. D. As (A), but ILA1 CTL were incubated with 50 nM dasatinib for various times prior to staining with pMHCI tetramer. For this experiment the drug was washed off prior to staining. E. As (A), but tetramer concentration was varied to stain CTL pre-treated ± 50 nM dasatinib for 30 min. F. 10 5 Mel13 CTL were stained with various concentrations of HLA A2/ELAGIGILTV tetramer following incubation ± 50 nM dasatinib for 30 min. G. 5x10 5 splenocytes from an F5 TCR transgenic Rag + mouse were resuspended in PBS ± 50 nM dasatinib and incubated for 30 min at 37 °C. Cells were subsequently stained with H2-D b /ASNENMDAM-PE tetramer for 20 min at 37 °C followed by anti-CD8 Cy5.5 for 30 min on ice prior to two washes in PBS and analysis by flow cytometry. H. 10 5 cells of the HLA DR⁎0101-restricted, influenza virus A HA 307-319 PKYVKQNTLKLAT-specific CD4 + clone C6 were incubated with PBS ± 50 nM dasatinib for 30 min at 37 °C, then stained with cognate PE-conjugated tetramer for 20 min at 37 °C. Samples were washed with PBS before flow cytometric analysis. Irrelevant tetramer was used as a negative control in all cases.

    Article Snippet: The cells were evaluated using a FACSCalibur flow cytometer (BD Biosciences).

    Techniques: Staining, CTL Assay, Incubation, Concentration Assay, Flow Cytometry, Cytometry, Fluorescence, Transgenic Assay, Negative Control

    Dasatinib treatment preferentially increases the ability of pMHCI tetramers to stain T-cells bearing low affinity TCRs. A. 10 5 ILA1 CTL were stained with 10μg/ml PE-conjugated HLA A2 tetramer folded around the 8E, 5Y, 4L, index (ILAKFLHWL), 3G8T or 3G peptides for 20 min at 37 °C following incubation ± 50 nM dasatinib for 30 min at 37 °C. For all samples, data were acquired with a FACSCalibur flow cytometer (BD) and analyzed using FlowJo software. Irrelevant tetramer was used as a negative control. B. The MFI of tetramer staining for all of the variants in the presence and absence of dasatinib displayed in (A) are plotted against the monomeric affinity of TCR/pMHCI interactions previously measured for each of these variants expressed as the dissociation constant (K D ) ( Table 1 ). Curves were fitted as described in the Materials and methods.

    Journal: Journal of Immunological Methods

    Article Title: Protein kinase inhibitors substantially improve the physical detection of T-cells with peptide-MHC tetramers

    doi: 10.1016/j.jim.2008.09.014

    Figure Lengend Snippet: Dasatinib treatment preferentially increases the ability of pMHCI tetramers to stain T-cells bearing low affinity TCRs. A. 10 5 ILA1 CTL were stained with 10μg/ml PE-conjugated HLA A2 tetramer folded around the 8E, 5Y, 4L, index (ILAKFLHWL), 3G8T or 3G peptides for 20 min at 37 °C following incubation ± 50 nM dasatinib for 30 min at 37 °C. For all samples, data were acquired with a FACSCalibur flow cytometer (BD) and analyzed using FlowJo software. Irrelevant tetramer was used as a negative control. B. The MFI of tetramer staining for all of the variants in the presence and absence of dasatinib displayed in (A) are plotted against the monomeric affinity of TCR/pMHCI interactions previously measured for each of these variants expressed as the dissociation constant (K D ) ( Table 1 ). Curves were fitted as described in the Materials and methods.

    Article Snippet: The cells were evaluated using a FACSCalibur flow cytometer (BD Biosciences).

    Techniques: Staining, CTL Assay, Incubation, Flow Cytometry, Cytometry, Software, Negative Control

    Non-cognate A2/K b -mediated CTL activation and tetramer binding is not influenced by MHCI restriction A. 2.5×10 5 PBMC were suspended in 250μl FACS buffer (2% FCS/PBS) and stained with FITC-conjugated anti-A2 and 7-AAD for 30 minutes on ice, then washed twice and resuspended in PBS. For pMHCI tetramer staining experiments, 2.5×10 5 PBMC were suspended in 50μl FACS buffer (2% FCS/PBS) and incubated +/− 10μg/ml unconjugated anti-CD8 for 20 minutes on ice, then stained with 10μg/ml of the PE-conjugated tetramers A2 ILAKFLHWL or A2/K b ILAKFLHWL for 45 minutes on ice. After washing, cells were subsequently stained with APC-conjugated anti-CD8 and 7-AAD, washed again and resuspended in PBS. Data were acquired using a FACSCalibur flow cytometer and analyzed with FlowJo software. B. 2.5×10 4 CTL were incubated for 12 hours at 37°C with 10 5 unpulsed C1R cells expressing either A2 or A2/K b on the cell surface. The following CTL clones were used: (i) the HLA A*6801-restricted CTL clone c23, specific for the HIV-1 Tat-derived epitope ITKGLGISYGR (residues 38-48); (ii) the HLA B*0702-restricted CTL clone KD4, specific for the EBV EBNA3A-derived epitope RPPIFIRRL (residues 379-387); (iii) the HLA B*0801-restricted CTL clone LC13, specific for the EBV EBNA3A-derived epitope FLRGRAYGL (residues 339-347); and, (iv) the HLA B*3508-restricted CTL clone SB27, specific for the EBV BZLF1-derived epitope LPEPLPQGQLTAY (residues 52-64). Supernatant was subsequently assayed for MIP-1β content by ELISA. The mean ± SD of two replicate assays is shown.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Major histocompatibility complex class I molecules with super-enhanced CD8 binding properties bypass the requirement for cognate TCR recognition and non-specifically activate cytotoxic T lymphocytes 1

    doi: 10.4049/jimmunol.0902398

    Figure Lengend Snippet: Non-cognate A2/K b -mediated CTL activation and tetramer binding is not influenced by MHCI restriction A. 2.5×10 5 PBMC were suspended in 250μl FACS buffer (2% FCS/PBS) and stained with FITC-conjugated anti-A2 and 7-AAD for 30 minutes on ice, then washed twice and resuspended in PBS. For pMHCI tetramer staining experiments, 2.5×10 5 PBMC were suspended in 50μl FACS buffer (2% FCS/PBS) and incubated +/− 10μg/ml unconjugated anti-CD8 for 20 minutes on ice, then stained with 10μg/ml of the PE-conjugated tetramers A2 ILAKFLHWL or A2/K b ILAKFLHWL for 45 minutes on ice. After washing, cells were subsequently stained with APC-conjugated anti-CD8 and 7-AAD, washed again and resuspended in PBS. Data were acquired using a FACSCalibur flow cytometer and analyzed with FlowJo software. B. 2.5×10 4 CTL were incubated for 12 hours at 37°C with 10 5 unpulsed C1R cells expressing either A2 or A2/K b on the cell surface. The following CTL clones were used: (i) the HLA A*6801-restricted CTL clone c23, specific for the HIV-1 Tat-derived epitope ITKGLGISYGR (residues 38-48); (ii) the HLA B*0702-restricted CTL clone KD4, specific for the EBV EBNA3A-derived epitope RPPIFIRRL (residues 379-387); (iii) the HLA B*0801-restricted CTL clone LC13, specific for the EBV EBNA3A-derived epitope FLRGRAYGL (residues 339-347); and, (iv) the HLA B*3508-restricted CTL clone SB27, specific for the EBV BZLF1-derived epitope LPEPLPQGQLTAY (residues 52-64). Supernatant was subsequently assayed for MIP-1β content by ELISA. The mean ± SD of two replicate assays is shown.

    Article Snippet: Data were acquired using a FACSCalibur or FACSAria™ II flow cytometer (BD) and analyzed with either CellQuest (BD BioSciences) or FlowJo (Tree Star Inc.) software.

    Techniques: CTL Assay, Activation Assay, Binding Assay, FACS, Staining, Incubation, Flow Cytometry, Cytometry, Software, Expressing, Clone Assay, Derivative Assay, Enzyme-linked Immunosorbent Assay

    A2/K b tetramers bind the majority of CTL in peripheral blood A. 2.5×10 5 PBMC from an A2 + donor were stained with PerCP-conjugated anti-CD8, 7-AAD and either FITC-conjugated anti-αβ-TCR, APC-conjugated anti-CD56 or PE-conjugated anti-γδ-TCR for 30 minutes on ice, washed twice and resuspended in PBS. B. 2.5×10 5 A2 + PBMC were stained with 10μg/ml of the PE-conjugated tetramers A2 ILAKFLHWL or A2/K b ILAKFLHWL for 20 minutes at 37°C. After washing, cells were subsequently stained with 7-AAD and either FITC-conjugated anti-γδ-TCR or FITC-conjugated anti-CD56 for 30 minutes on ice, washed twice and resuspended in PBS. C. 2.5×10 5 A2 + PBMC were stained with 10μg/ml of the PE-conjugated tetramers A2 ILAKFLHWL or A2/K b ILAKFLHWL for 20 minutes at 37°C. After washing, cells were stained with APC-conjugated anti-CD8, FITC-conjugated anti-αβ-TCR and 7-AAD for 30 minutes on ice, washed twice and resuspended in PBS. In A , B and C , data were acquired using a FACSCalibur flow cytometer and analyzed with FlowJo software.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Major histocompatibility complex class I molecules with super-enhanced CD8 binding properties bypass the requirement for cognate TCR recognition and non-specifically activate cytotoxic T lymphocytes 1

    doi: 10.4049/jimmunol.0902398

    Figure Lengend Snippet: A2/K b tetramers bind the majority of CTL in peripheral blood A. 2.5×10 5 PBMC from an A2 + donor were stained with PerCP-conjugated anti-CD8, 7-AAD and either FITC-conjugated anti-αβ-TCR, APC-conjugated anti-CD56 or PE-conjugated anti-γδ-TCR for 30 minutes on ice, washed twice and resuspended in PBS. B. 2.5×10 5 A2 + PBMC were stained with 10μg/ml of the PE-conjugated tetramers A2 ILAKFLHWL or A2/K b ILAKFLHWL for 20 minutes at 37°C. After washing, cells were subsequently stained with 7-AAD and either FITC-conjugated anti-γδ-TCR or FITC-conjugated anti-CD56 for 30 minutes on ice, washed twice and resuspended in PBS. C. 2.5×10 5 A2 + PBMC were stained with 10μg/ml of the PE-conjugated tetramers A2 ILAKFLHWL or A2/K b ILAKFLHWL for 20 minutes at 37°C. After washing, cells were stained with APC-conjugated anti-CD8, FITC-conjugated anti-αβ-TCR and 7-AAD for 30 minutes on ice, washed twice and resuspended in PBS. In A , B and C , data were acquired using a FACSCalibur flow cytometer and analyzed with FlowJo software.

    Article Snippet: Data were acquired using a FACSCalibur or FACSAria™ II flow cytometer (BD) and analyzed with either CellQuest (BD BioSciences) or FlowJo (Tree Star Inc.) software.

    Techniques: CTL Assay, Staining, Flow Cytometry, Cytometry, Software

    Non-specific A2/K b tetramer binding is influenced by CD8 cell surface density A and B. 2×10 5 293T cells were incubated +/− 10 μg/ml of the PE-conjugated tetramers A2 D227K/T228A ILAKFLHWL, A2 ILAKFLHWL or A2/K b ILAKFLHWL for 20 minutes at 37°C, then stained with 7-AAD and either FITC-conjugated anti-CD8 or PE-conjugated anti-CD8β for 30 minutes on ice, washed twice and resuspended in PBS. C. 2.5×10 5 PBMC were stained with PerCP-conjugated anti-CD8, 7-AAD and either FITC-conjugated anti-αβ-TCR, APC-conjugated anti-CD56 or PE-conjugated anti-γδ-TCR for 30 minutes on ice, washed twice and resuspended in PBS. In A , B and C , data were acquired using a FACSCalibur flow cytometer and analyzed with FlowJo software.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Major histocompatibility complex class I molecules with super-enhanced CD8 binding properties bypass the requirement for cognate TCR recognition and non-specifically activate cytotoxic T lymphocytes 1

    doi: 10.4049/jimmunol.0902398

    Figure Lengend Snippet: Non-specific A2/K b tetramer binding is influenced by CD8 cell surface density A and B. 2×10 5 293T cells were incubated +/− 10 μg/ml of the PE-conjugated tetramers A2 D227K/T228A ILAKFLHWL, A2 ILAKFLHWL or A2/K b ILAKFLHWL for 20 minutes at 37°C, then stained with 7-AAD and either FITC-conjugated anti-CD8 or PE-conjugated anti-CD8β for 30 minutes on ice, washed twice and resuspended in PBS. C. 2.5×10 5 PBMC were stained with PerCP-conjugated anti-CD8, 7-AAD and either FITC-conjugated anti-αβ-TCR, APC-conjugated anti-CD56 or PE-conjugated anti-γδ-TCR for 30 minutes on ice, washed twice and resuspended in PBS. In A , B and C , data were acquired using a FACSCalibur flow cytometer and analyzed with FlowJo software.

    Article Snippet: Data were acquired using a FACSCalibur or FACSAria™ II flow cytometer (BD) and analyzed with either CellQuest (BD BioSciences) or FlowJo (Tree Star Inc.) software.

    Techniques: Binding Assay, Incubation, Staining, Flow Cytometry, Cytometry, Software

    A2/K b tetramers can activate CTL in the absence of a specific TCR/pMHCI interaction A. 10 5 003 CTL were suspended in 20μl PBS and stained with the PE-conjugated tetramers A2 SLYNTVATL, A2/K b SLYNTVATL, A2 LLFGYPVYV or A2/K b LLFGYPVYV at the indicated concentrations and 7-AAD for 20 minutes at 37°C. Cells were then washed twice and resuspended in PBS. Data were acquired using a FACSCalibur flow cytometer and analyzed with CellQuest software. B. 10 5 003 CTL were suspended in 40μl R2 with the PE-conjugated tetramers A2 SLYNTVATL, A2/K b SLYNTVATL, A2 LLFGYPVYV or A2/K b LLFGYPVYV at the indicated concentrations for 30 minutes at 37°C. Cells were subsequently stained with FITC-conjugated anti-αβ-TCR, 7-AAD and APC-conjugated anti-CD8 for 30 minutes on ice in azide buffer (0.1% azide/2% FCS/PBS). After two washes, data were acquired using a FACSCalibur flow cytometer and analyzed with CellQuest software. C. 5×10 5 003 CTL were incubated with the PE-conjugated tetramers A2 SLYNTVATL, A2/K b SLYNTVATL, A2 LLFGYPVYV or A2/K b LLFGYPVYV at the indicated concentrations. After 4 hours at 37°C, supernatants were harvested and assayed for RANTES, IFNγ and MIP-1β content by ELISA (only RANTES shown). D. 2×10 3 868 CTL were incubated for 4 hours at 37°C with 1μg/ml of the PE-conjugated tetramers A2 SLYNTVATL, A2/K b SLYNTVATL, A2 LLFGYPVYV or A2/K b LLFGYPVYV in an IFNγ ELISpot assay. E. 1.25×10 5 868 CTL were incubated with 1μg/ml of the PE-conjugated tetramers A2 SLYNTVATL, A2/K b SLYNTVATL, A2 LLFGYPVYV or A2/K b LLFGYPVYV for 4 hours at 37°C. The supernatant was subsequently assayed for MIP-1β content by ELISA. Figures ( C-E ) show the mean ± SD of two replicate assays. Results similar to ( A-E ) were also obtained with tetramers conjugated to fluorochromes other than PE (data not shown). F. 003 CTL were incubated with the PE-conjugated tetramers A2 SLYNTVATL, A2/K b SLYNTVATL, A2 GLCTLVAML or A2/K b GLCTLVAML at the indicated concentrations for 4 hours at 37°C, then stained with APC-conjugated anti-CD8 for 20 minutes on ice and assayed for CD107a mobilization as described in the Materials and Methods. The inset plot shows staining for APC-conjugated anti-CD8 on the x-axis and PE-conjugated A2/K b GLCTLVAML tetramer (5μg/ml) on the y-axis. Back-gated tetramer + CD107a + cells are shown in black and tetramer + CD107a − cells are shown in grey. Tetramer high CD8 high cells are preferentially activated by the A2/K b tetramer.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Major histocompatibility complex class I molecules with super-enhanced CD8 binding properties bypass the requirement for cognate TCR recognition and non-specifically activate cytotoxic T lymphocytes 1

    doi: 10.4049/jimmunol.0902398

    Figure Lengend Snippet: A2/K b tetramers can activate CTL in the absence of a specific TCR/pMHCI interaction A. 10 5 003 CTL were suspended in 20μl PBS and stained with the PE-conjugated tetramers A2 SLYNTVATL, A2/K b SLYNTVATL, A2 LLFGYPVYV or A2/K b LLFGYPVYV at the indicated concentrations and 7-AAD for 20 minutes at 37°C. Cells were then washed twice and resuspended in PBS. Data were acquired using a FACSCalibur flow cytometer and analyzed with CellQuest software. B. 10 5 003 CTL were suspended in 40μl R2 with the PE-conjugated tetramers A2 SLYNTVATL, A2/K b SLYNTVATL, A2 LLFGYPVYV or A2/K b LLFGYPVYV at the indicated concentrations for 30 minutes at 37°C. Cells were subsequently stained with FITC-conjugated anti-αβ-TCR, 7-AAD and APC-conjugated anti-CD8 for 30 minutes on ice in azide buffer (0.1% azide/2% FCS/PBS). After two washes, data were acquired using a FACSCalibur flow cytometer and analyzed with CellQuest software. C. 5×10 5 003 CTL were incubated with the PE-conjugated tetramers A2 SLYNTVATL, A2/K b SLYNTVATL, A2 LLFGYPVYV or A2/K b LLFGYPVYV at the indicated concentrations. After 4 hours at 37°C, supernatants were harvested and assayed for RANTES, IFNγ and MIP-1β content by ELISA (only RANTES shown). D. 2×10 3 868 CTL were incubated for 4 hours at 37°C with 1μg/ml of the PE-conjugated tetramers A2 SLYNTVATL, A2/K b SLYNTVATL, A2 LLFGYPVYV or A2/K b LLFGYPVYV in an IFNγ ELISpot assay. E. 1.25×10 5 868 CTL were incubated with 1μg/ml of the PE-conjugated tetramers A2 SLYNTVATL, A2/K b SLYNTVATL, A2 LLFGYPVYV or A2/K b LLFGYPVYV for 4 hours at 37°C. The supernatant was subsequently assayed for MIP-1β content by ELISA. Figures ( C-E ) show the mean ± SD of two replicate assays. Results similar to ( A-E ) were also obtained with tetramers conjugated to fluorochromes other than PE (data not shown). F. 003 CTL were incubated with the PE-conjugated tetramers A2 SLYNTVATL, A2/K b SLYNTVATL, A2 GLCTLVAML or A2/K b GLCTLVAML at the indicated concentrations for 4 hours at 37°C, then stained with APC-conjugated anti-CD8 for 20 minutes on ice and assayed for CD107a mobilization as described in the Materials and Methods. The inset plot shows staining for APC-conjugated anti-CD8 on the x-axis and PE-conjugated A2/K b GLCTLVAML tetramer (5μg/ml) on the y-axis. Back-gated tetramer + CD107a + cells are shown in black and tetramer + CD107a − cells are shown in grey. Tetramer high CD8 high cells are preferentially activated by the A2/K b tetramer.

    Article Snippet: Data were acquired using a FACSCalibur or FACSAria™ II flow cytometer (BD) and analyzed with either CellQuest (BD BioSciences) or FlowJo (Tree Star Inc.) software.

    Techniques: CTL Assay, Staining, Flow Cytometry, Cytometry, Software, Incubation, Enzyme-linked Immunosorbent Assay, Enzyme-linked Immunospot

    Cell surface-expressed A2/K b primes non-specific expansion of CD8 + cells 10 6 A2 + PBMC were incubated with 2×10 5 irradiated A2 D227K/T228A, A2 or A2/K b C1R cells that had previously been pulsed with 1μM ELAGIGILTV (Melan-A 26-35 ) peptide in R10. From day 3, IL-2 was added in increments to reach a maximum concentration of 200 IU/ml by day 10. Lines were subsequently stained with PE-conjugated A2 ELAGIGILTV tetramer followed by APC-conjugated anti-CD8 and 7-AAD. Data were acquired using a FACSCalibur flow cytometer and analyzed with FlowJo software.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Major histocompatibility complex class I molecules with super-enhanced CD8 binding properties bypass the requirement for cognate TCR recognition and non-specifically activate cytotoxic T lymphocytes 1

    doi: 10.4049/jimmunol.0902398

    Figure Lengend Snippet: Cell surface-expressed A2/K b primes non-specific expansion of CD8 + cells 10 6 A2 + PBMC were incubated with 2×10 5 irradiated A2 D227K/T228A, A2 or A2/K b C1R cells that had previously been pulsed with 1μM ELAGIGILTV (Melan-A 26-35 ) peptide in R10. From day 3, IL-2 was added in increments to reach a maximum concentration of 200 IU/ml by day 10. Lines were subsequently stained with PE-conjugated A2 ELAGIGILTV tetramer followed by APC-conjugated anti-CD8 and 7-AAD. Data were acquired using a FACSCalibur flow cytometer and analyzed with FlowJo software.

    Article Snippet: Data were acquired using a FACSCalibur or FACSAria™ II flow cytometer (BD) and analyzed with either CellQuest (BD BioSciences) or FlowJo (Tree Star Inc.) software.

    Techniques: Incubation, Irradiation, Concentration Assay, Staining, Flow Cytometry, Cytometry, Software

    The exquisite specificity of pMHCI tetramer staining is lost when the strength of the pMHCI/CD8 interaction is increased by ~15-fold A. The 003 or NT1 CTL clones (10 5 cells) or the 868 CTL line (2.5×10 5 cells), all specific for HIV-1 p17 Gag 77-85 , were stained with 1μg of the PE-conjugated tetramers A2 SLYNTVATL, A2/K b SLYNTVATL, A2 LLFGYPVYV or A2/K b LLFGYPVYV in 20μl PBS for 20 minutes at 37°C. Cells were then stained with APC-conjugated anti-CD8 and 7-AAD for 30 minutes on ice, washed twice and resuspended in PBS. Data were acquired using a FACSCalibur flow cytometer and analyzed with CellQuest software. B. 2.5×10 5 PBMC were suspended in 250 μl FACS buffer (2% FCS/PBS), then stained with 1μg of the PE-conjugated tetramers A2 SLYNTVATL, A2/K b SLYNTVATL, A2 LLFGYPVYV or A2/K b LLFGYPVYV for 20 minutes at 37°C. Each sample was subsequently stained with APC-conjugated anti-CD8, PerCP-conjugated anti-CD3 and 7-AAD for 30 minutes on ice, washed twice and resuspended in FACS buffer. Data were acquired using a FACSCalibur flow cytometer and analysed with CellQuest software by gating on the live CD3 + population. The values shown represent the percent of CD3 + CD8 + cells that stain with the indicated tetramer.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Major histocompatibility complex class I molecules with super-enhanced CD8 binding properties bypass the requirement for cognate TCR recognition and non-specifically activate cytotoxic T lymphocytes 1

    doi: 10.4049/jimmunol.0902398

    Figure Lengend Snippet: The exquisite specificity of pMHCI tetramer staining is lost when the strength of the pMHCI/CD8 interaction is increased by ~15-fold A. The 003 or NT1 CTL clones (10 5 cells) or the 868 CTL line (2.5×10 5 cells), all specific for HIV-1 p17 Gag 77-85 , were stained with 1μg of the PE-conjugated tetramers A2 SLYNTVATL, A2/K b SLYNTVATL, A2 LLFGYPVYV or A2/K b LLFGYPVYV in 20μl PBS for 20 minutes at 37°C. Cells were then stained with APC-conjugated anti-CD8 and 7-AAD for 30 minutes on ice, washed twice and resuspended in PBS. Data were acquired using a FACSCalibur flow cytometer and analyzed with CellQuest software. B. 2.5×10 5 PBMC were suspended in 250 μl FACS buffer (2% FCS/PBS), then stained with 1μg of the PE-conjugated tetramers A2 SLYNTVATL, A2/K b SLYNTVATL, A2 LLFGYPVYV or A2/K b LLFGYPVYV for 20 minutes at 37°C. Each sample was subsequently stained with APC-conjugated anti-CD8, PerCP-conjugated anti-CD3 and 7-AAD for 30 minutes on ice, washed twice and resuspended in FACS buffer. Data were acquired using a FACSCalibur flow cytometer and analysed with CellQuest software by gating on the live CD3 + population. The values shown represent the percent of CD3 + CD8 + cells that stain with the indicated tetramer.

    Article Snippet: Data were acquired using a FACSCalibur or FACSAria™ II flow cytometer (BD) and analyzed with either CellQuest (BD BioSciences) or FlowJo (Tree Star Inc.) software.

    Techniques: Staining, CTL Assay, Clone Assay, Flow Cytometry, Cytometry, Software, FACS

    Analysis of transfection efficiency and correlation between transfection efficiency and vector size. The five constructed vectors were transfected into CHO cells using Lipofectamine ® 3000 Transfection Reagent. The transfection efficiency was analysed using a FACSCalibur cytometer. ( A ) Analysis of the transfection efficiency. Three independent experiments were performed in this study. Standard error of the mean (S.E.M.) is indicated. ( B ) Correlation between transfection efficiency and vector size. Transfection efficiency analysed by FACSCalibur cytometer decreased exponentially as vector size increased. The graph was created with Microsoft Office Excel.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: SV40 intron, a potent strong intron element that effectively increases transgene expression in transfected Chinese hamster ovary cells

    doi: 10.1111/jcmm.13504

    Figure Lengend Snippet: Analysis of transfection efficiency and correlation between transfection efficiency and vector size. The five constructed vectors were transfected into CHO cells using Lipofectamine ® 3000 Transfection Reagent. The transfection efficiency was analysed using a FACSCalibur cytometer. ( A ) Analysis of the transfection efficiency. Three independent experiments were performed in this study. Standard error of the mean (S.E.M.) is indicated. ( B ) Correlation between transfection efficiency and vector size. Transfection efficiency analysed by FACSCalibur cytometer decreased exponentially as vector size increased. The graph was created with Microsoft Office Excel.

    Article Snippet: After 48 hrs transfection, the transfection efficiency and eGFP mean fluorescence intensity (MFI) of each vector were analysed using a FACSCalibur cytometer (Becton Dickinson, Franklin Lakes, NJ, USA), the untransfected cells used as the negative control.

    Techniques: Transfection, Plasmid Preparation, Construct, Cytometry

    Recombinant protein transient expression of different intron elements. The constructed vectors were transfected into CHO cells, and eGFP expression levels were estimated by fluorescence microscopy at 48 hrs after transfection and recombinant protein transient expression was determined at 48 hrs after transfection with the FACSCalibur. ( A ) eGFP transient expression observed by fluorescence microscopy. ( B ) Analysis of the transient expression of different intron elements. Three independent experiments were performed in this study. These results are the mean values obtained for three independent experiments. Standard error of the mean (S.E.M.) is indicated (Student's t ‐test, * P

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: SV40 intron, a potent strong intron element that effectively increases transgene expression in transfected Chinese hamster ovary cells

    doi: 10.1111/jcmm.13504

    Figure Lengend Snippet: Recombinant protein transient expression of different intron elements. The constructed vectors were transfected into CHO cells, and eGFP expression levels were estimated by fluorescence microscopy at 48 hrs after transfection and recombinant protein transient expression was determined at 48 hrs after transfection with the FACSCalibur. ( A ) eGFP transient expression observed by fluorescence microscopy. ( B ) Analysis of the transient expression of different intron elements. Three independent experiments were performed in this study. These results are the mean values obtained for three independent experiments. Standard error of the mean (S.E.M.) is indicated (Student's t ‐test, * P

    Article Snippet: After 48 hrs transfection, the transfection efficiency and eGFP mean fluorescence intensity (MFI) of each vector were analysed using a FACSCalibur cytometer (Becton Dickinson, Franklin Lakes, NJ, USA), the untransfected cells used as the negative control.

    Techniques: Recombinant, Expressing, Construct, Transfection, Fluorescence, Microscopy

    Effect of different introns on the production of eGFP in stable transfected CHO‐S cell. ( A ) Relative eGFP mRNA levels in stable transfected cells. At 48 hrs after transfection, stable transfected cells were selected by 800 μg/ml geneticin selection for 2 weeks until positive colonies appeared. Then, stable cell populations exhibiting stable transgene integration were cultured in CD CHO medium supplemented with 8 mM l ‐glutamine in 125‐ml Corning shake flasks with 30 ml medium with the presence of 500 μg/ml G418 for 30 days. Total RNA was isolated from 5 × 10 6 cells using the RNApure Tissue Kit, analysis of the mRNA levels was determined using real‐time quantitative PCR. ( B ) eGFP MFI was determined by cytometry for stable transfected cell pool with the different introns. Cells were collected and the eGFP fluorescence profile was measured for by FACSCalibur at 30 days after transfection. ( C ) eGFP MFI expression was normalized to IVS, and in the statistical analysis of eGFP expression, fold change values were normalized to those of the IVS, whose value was set to 1. Three independent experiments were performed in this study. Standard error of the mean (S.E.M.) is indicated (Student's t ‐test, * P

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: SV40 intron, a potent strong intron element that effectively increases transgene expression in transfected Chinese hamster ovary cells

    doi: 10.1111/jcmm.13504

    Figure Lengend Snippet: Effect of different introns on the production of eGFP in stable transfected CHO‐S cell. ( A ) Relative eGFP mRNA levels in stable transfected cells. At 48 hrs after transfection, stable transfected cells were selected by 800 μg/ml geneticin selection for 2 weeks until positive colonies appeared. Then, stable cell populations exhibiting stable transgene integration were cultured in CD CHO medium supplemented with 8 mM l ‐glutamine in 125‐ml Corning shake flasks with 30 ml medium with the presence of 500 μg/ml G418 for 30 days. Total RNA was isolated from 5 × 10 6 cells using the RNApure Tissue Kit, analysis of the mRNA levels was determined using real‐time quantitative PCR. ( B ) eGFP MFI was determined by cytometry for stable transfected cell pool with the different introns. Cells were collected and the eGFP fluorescence profile was measured for by FACSCalibur at 30 days after transfection. ( C ) eGFP MFI expression was normalized to IVS, and in the statistical analysis of eGFP expression, fold change values were normalized to those of the IVS, whose value was set to 1. Three independent experiments were performed in this study. Standard error of the mean (S.E.M.) is indicated (Student's t ‐test, * P

    Article Snippet: After 48 hrs transfection, the transfection efficiency and eGFP mean fluorescence intensity (MFI) of each vector were analysed using a FACSCalibur cytometer (Becton Dickinson, Franklin Lakes, NJ, USA), the untransfected cells used as the negative control.

    Techniques: Transfection, Selection, Stable Transfection, Cell Culture, Isolation, Real-time Polymerase Chain Reaction, Cytometry, Fluorescence, Expressing

    Passing and Bablok regression between the BD FACSPresto™ with capillary blood (a-c) and venous blood (d-f) compared to the BD FACSCalibur™. Comparison of the BD FACSPresto™ with BD FACSCalibur™ for CD4+ T-cell count with capillary blood (a) and with venous blood samples (d). Comparison of the BD FACSPresto™ with BD FACSCalibur™ for CD4% with capillary blood (b) and with venous blood samples (e). Comparison of the BD FACSPresto™ with Sysmex XT-1800i™with capillary blood (c) and with venous blood (f).

    Journal: PLoS ONE

    Article Title: The performance of BD FACSPresto™ for CD4 T-cell count, CD4% and hemoglobin concentration test in Ethiopia

    doi: 10.1371/journal.pone.0176323

    Figure Lengend Snippet: Passing and Bablok regression between the BD FACSPresto™ with capillary blood (a-c) and venous blood (d-f) compared to the BD FACSCalibur™. Comparison of the BD FACSPresto™ with BD FACSCalibur™ for CD4+ T-cell count with capillary blood (a) and with venous blood samples (d). Comparison of the BD FACSPresto™ with BD FACSCalibur™ for CD4% with capillary blood (b) and with venous blood samples (e). Comparison of the BD FACSPresto™ with Sysmex XT-1800i™with capillary blood (c) and with venous blood (f).

    Article Snippet: CD4% comparison between the BD FACSPresto™ (venous blood) and BD FACSCalibur™ The BD FACSPresto™ with venous blood had an absolute mean bias of 0.8 (0.2%) (95% LOA: -1.8, 3.4) ( ) compared to the BD FACSCalibur™.

    Techniques: Cell Counting

    Bland-Altman comparisons between the BD FACSPresto™ with capillary blood (a-c) and venous blood (d-f) samples with the BD FACSCalibur™ reference standard. The corresponding graphs show the absolute bias between the FACSPresto™ and FACSCalibur represented in the Bland-Altman plots for CD4 T-cell testing with capillary blood (a) and venous blood (d); Bland-Altman plots for CD4% testing with capillary blood (b), venous blood (e) samples; Bland-Altman plots for Hgb testing with capillary blood (c), venous blood (f)., The solid green lines represent the mean bias and the solid deep red lines represent the upper and lower limits of agreement (LOA = mean ± 1.96SD).

    Journal: PLoS ONE

    Article Title: The performance of BD FACSPresto™ for CD4 T-cell count, CD4% and hemoglobin concentration test in Ethiopia

    doi: 10.1371/journal.pone.0176323

    Figure Lengend Snippet: Bland-Altman comparisons between the BD FACSPresto™ with capillary blood (a-c) and venous blood (d-f) samples with the BD FACSCalibur™ reference standard. The corresponding graphs show the absolute bias between the FACSPresto™ and FACSCalibur represented in the Bland-Altman plots for CD4 T-cell testing with capillary blood (a) and venous blood (d); Bland-Altman plots for CD4% testing with capillary blood (b), venous blood (e) samples; Bland-Altman plots for Hgb testing with capillary blood (c), venous blood (f)., The solid green lines represent the mean bias and the solid deep red lines represent the upper and lower limits of agreement (LOA = mean ± 1.96SD).

    Article Snippet: CD4% comparison between the BD FACSPresto™ (venous blood) and BD FACSCalibur™ The BD FACSPresto™ with venous blood had an absolute mean bias of 0.8 (0.2%) (95% LOA: -1.8, 3.4) ( ) compared to the BD FACSCalibur™.

    Techniques: