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    Becton Dickinson facsaria ii
    Image-based capture of individual spiked cells . (A) Cell surface GD2 expression was measured by flow cytometry for the eight neuroblastoma cell lines used for isolation of single cells (and listed at right), as compared to negative control WBCs and isotype control. (B) WBCs and the GD2-dim neuroblastoma cell line SY5Y were mixed at a tumor:WBC ratio of 1:35, pre-labeled with GD2-PE, CD45-FITC, and Hoechst nuclear dye, then injected into the DEPArray. Shown are bright-field images of (i) a single tumor cell (bar depicts 10 μm) and (ii) a cluster of two cells. In (C) , a scatter plot of GD2-PE and CD45-FITC mean fluorescence intensity (MFI) is shown as well as cell images, including on the left-hand side of the dot-plot (i) an image of a single tumor cell and (ii) a single WBC, and on the right-hand side (iii) a heterogeneous cluster, (iv) a homogeneous cluster, and (v) a spurious event. (D) NB1643M cells and normal donor WBCs were mixed at a ratio of 1:1,000,000 and stained with GD2-PE and CD45-FITC, in this representative experiment. The sample was pre-enriched using <t>FACSAria-based</t> sorting, and the enriched fraction placed into the DEPArray cartridge. Shown are a scatter plot of GD2-PE and CD45-FITC MFI, as well as images of (i–iv) intact tumor cells, (v and vi) WBCs, and (vii–ix) debris and false-positive events.
    Facsaria Ii, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Becton Dickinson facsaria ii cell sorter
    Bone marrow B cell flow cytometry results from a healthy donor. First, B cells were gated by using specific cell surface markers that were CD138 + and CD38 + by determining forward and side light scattering characteristics on the <t>FACSAria</t> II Cell Sorter (Becton Dickinson, San Jose, CA, USA). Then sorted B cells using with cell sorting by the cell surface markers CD56 + , CD19 + according to the FACSAria II Cell Sorter.
    Facsaria Ii Cell Sorter, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Becton Dickinson facsaria ii flow cytometer
    Bone marrow B cell flow cytometry results from a healthy donor. First, B cells were gated by using specific cell surface markers that were CD138 + and CD38 + by determining forward and side light scattering characteristics on the <t>FACSAria</t> II Cell Sorter (Becton Dickinson, San Jose, CA, USA). Then sorted B cells using with cell sorting by the cell surface markers CD56 + , CD19 + according to the FACSAria II Cell Sorter.
    Facsaria Ii Flow Cytometer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Becton Dickinson bd facsaria ii
    Adjuvanticity of MDP, chitosan, Pam 3 CSK 4 , MPL, and iE-DAP to OVA in OVA-specific TCR transgenic mice. Naive OVA-specific T cells were purified from the spleens and inguinal lymph nodes of CD45.1×OTII F 1 mice and transferred into female C57BL/6J mice. One day later, the recipients were immunized via buccal mucosal injection with one of seven compositions in 40 μl, namely, 1 μg of OVA, OVA plus 40 μg of MDP, OVA plus 32 μg of chitosan, OVA plus 25 μg of Pam 3 CSK 4 , OVA plus 15 μg of MPL, OVA plus 50 μg of iE-DAP, or PBS control. Three days later, the mice were sacrificed. The draining submandibular lymph nodes were isolated, and the cells were counted (A). Single-cell suspensions were made for staining with CD45.1 plus CD4 and then run on a BD <t>FACSAria</t> II and analyzed for the number of OVA-specific T cells (B). *, Significantly different from the PBS group (*, P
    Bd Facsaria Ii, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Image-based capture of individual spiked cells . (A) Cell surface GD2 expression was measured by flow cytometry for the eight neuroblastoma cell lines used for isolation of single cells (and listed at right), as compared to negative control WBCs and isotype control. (B) WBCs and the GD2-dim neuroblastoma cell line SY5Y were mixed at a tumor:WBC ratio of 1:35, pre-labeled with GD2-PE, CD45-FITC, and Hoechst nuclear dye, then injected into the DEPArray. Shown are bright-field images of (i) a single tumor cell (bar depicts 10 μm) and (ii) a cluster of two cells. In (C) , a scatter plot of GD2-PE and CD45-FITC mean fluorescence intensity (MFI) is shown as well as cell images, including on the left-hand side of the dot-plot (i) an image of a single tumor cell and (ii) a single WBC, and on the right-hand side (iii) a heterogeneous cluster, (iv) a homogeneous cluster, and (v) a spurious event. (D) NB1643M cells and normal donor WBCs were mixed at a ratio of 1:1,000,000 and stained with GD2-PE and CD45-FITC, in this representative experiment. The sample was pre-enriched using FACSAria-based sorting, and the enriched fraction placed into the DEPArray cartridge. Shown are a scatter plot of GD2-PE and CD45-FITC MFI, as well as images of (i–iv) intact tumor cells, (v and vi) WBCs, and (vii–ix) debris and false-positive events.

    Journal: Frontiers in Oncology

    Article Title: Dielectrophoretic Capture and Genetic Analysis of Single Neuroblastoma Tumor Cells

    doi: 10.3389/fonc.2014.00201

    Figure Lengend Snippet: Image-based capture of individual spiked cells . (A) Cell surface GD2 expression was measured by flow cytometry for the eight neuroblastoma cell lines used for isolation of single cells (and listed at right), as compared to negative control WBCs and isotype control. (B) WBCs and the GD2-dim neuroblastoma cell line SY5Y were mixed at a tumor:WBC ratio of 1:35, pre-labeled with GD2-PE, CD45-FITC, and Hoechst nuclear dye, then injected into the DEPArray. Shown are bright-field images of (i) a single tumor cell (bar depicts 10 μm) and (ii) a cluster of two cells. In (C) , a scatter plot of GD2-PE and CD45-FITC mean fluorescence intensity (MFI) is shown as well as cell images, including on the left-hand side of the dot-plot (i) an image of a single tumor cell and (ii) a single WBC, and on the right-hand side (iii) a heterogeneous cluster, (iv) a homogeneous cluster, and (v) a spurious event. (D) NB1643M cells and normal donor WBCs were mixed at a ratio of 1:1,000,000 and stained with GD2-PE and CD45-FITC, in this representative experiment. The sample was pre-enriched using FACSAria-based sorting, and the enriched fraction placed into the DEPArray cartridge. Shown are a scatter plot of GD2-PE and CD45-FITC MFI, as well as images of (i–iv) intact tumor cells, (v and vi) WBCs, and (vii–ix) debris and false-positive events.

    Article Snippet: Due to the 40,000 cell capacity of the DEPArray cartridge, pre-enrichment of the sample was first completed on the FACSAria II (Becton Dickinson Franklin Lakes, NJ, USA) prior to running on the DEPArray, if the tumor cell concentration was below 1 in 5,000 WBCs (0.02%).

    Techniques: Expressing, Flow Cytometry, Cytometry, Isolation, Negative Control, Labeling, Injection, Fluorescence, Staining

    Isolation and targeted sequencing of rare individual patient DTC . (A) The post-Ficoll fraction for a bone marrow sample from patient CHOP7 was fixed, stained for CD45, CD56, and GD2, and an aliquot was analyzed by flow cytometry. Given that only 0.02% of cells were determined to be GD2-positive, (B) pre-enrichment was conducted on the FACSAria using the wide P2 gate shown. (C) Representative images of staining for GD2 (first column; bar depicts 10 μm), CD56 (middle column), and CD45 (right column) are shown for single cells #15 (top row) and #7 (bottom row). (D) A gel for the quality control panel of four housekeeping genes is shown with a “+” below depicting detection of either the mutant allele (top row labeled “M” or the wild-type allele bottom row labeled “WT”). (E) The chromatogram of the wild-type (top) and mutant allele (bottom) for cell #8. (F) Diameter of patient cells, as measured for 56 tumor cells and 22 WBCs while in solution on the DEPArray chip. (G) Bone marrow samples of patients were processed as described above and direct fluorescent staining used to measure the DNA concentration of WGA product from single cells. Mean WGA yield for n = 64 patient single cells is shown in comparison to the yield for n = 31 single tumor cells from cell line spiking experiments. In this analysis, all patient and cell line cells were fixed.

    Journal: Frontiers in Oncology

    Article Title: Dielectrophoretic Capture and Genetic Analysis of Single Neuroblastoma Tumor Cells

    doi: 10.3389/fonc.2014.00201

    Figure Lengend Snippet: Isolation and targeted sequencing of rare individual patient DTC . (A) The post-Ficoll fraction for a bone marrow sample from patient CHOP7 was fixed, stained for CD45, CD56, and GD2, and an aliquot was analyzed by flow cytometry. Given that only 0.02% of cells were determined to be GD2-positive, (B) pre-enrichment was conducted on the FACSAria using the wide P2 gate shown. (C) Representative images of staining for GD2 (first column; bar depicts 10 μm), CD56 (middle column), and CD45 (right column) are shown for single cells #15 (top row) and #7 (bottom row). (D) A gel for the quality control panel of four housekeeping genes is shown with a “+” below depicting detection of either the mutant allele (top row labeled “M” or the wild-type allele bottom row labeled “WT”). (E) The chromatogram of the wild-type (top) and mutant allele (bottom) for cell #8. (F) Diameter of patient cells, as measured for 56 tumor cells and 22 WBCs while in solution on the DEPArray chip. (G) Bone marrow samples of patients were processed as described above and direct fluorescent staining used to measure the DNA concentration of WGA product from single cells. Mean WGA yield for n = 64 patient single cells is shown in comparison to the yield for n = 31 single tumor cells from cell line spiking experiments. In this analysis, all patient and cell line cells were fixed.

    Article Snippet: Due to the 40,000 cell capacity of the DEPArray cartridge, pre-enrichment of the sample was first completed on the FACSAria II (Becton Dickinson Franklin Lakes, NJ, USA) prior to running on the DEPArray, if the tumor cell concentration was below 1 in 5,000 WBCs (0.02%).

    Techniques: Isolation, Sequencing, Staining, Flow Cytometry, Cytometry, Mutagenesis, Labeling, Chromatin Immunoprecipitation, Concentration Assay, Whole Genome Amplification

    Workflow for rare single cell isolation by DEPArray . Shown are the steps for dielectrophoretic isolation and downstream genetic analysis of single cells. Because the DEPArray cartridge has a capacity of ~40,000 cells, if the tumor cell concentration is below 0.02%, pre-enrichment of the sample is first completed on the FACSAria.

    Journal: Frontiers in Oncology

    Article Title: Dielectrophoretic Capture and Genetic Analysis of Single Neuroblastoma Tumor Cells

    doi: 10.3389/fonc.2014.00201

    Figure Lengend Snippet: Workflow for rare single cell isolation by DEPArray . Shown are the steps for dielectrophoretic isolation and downstream genetic analysis of single cells. Because the DEPArray cartridge has a capacity of ~40,000 cells, if the tumor cell concentration is below 0.02%, pre-enrichment of the sample is first completed on the FACSAria.

    Article Snippet: Due to the 40,000 cell capacity of the DEPArray cartridge, pre-enrichment of the sample was first completed on the FACSAria II (Becton Dickinson Franklin Lakes, NJ, USA) prior to running on the DEPArray, if the tumor cell concentration was below 1 in 5,000 WBCs (0.02%).

    Techniques: Single-cell Isolation, Isolation, Concentration Assay

    Bone marrow B cell flow cytometry results from a healthy donor. First, B cells were gated by using specific cell surface markers that were CD138 + and CD38 + by determining forward and side light scattering characteristics on the FACSAria II Cell Sorter (Becton Dickinson, San Jose, CA, USA). Then sorted B cells using with cell sorting by the cell surface markers CD56 + , CD19 + according to the FACSAria II Cell Sorter.

    Journal: Balkan Medical Journal

    Article Title: Investigation of Gene Expressions of Myeloma Cells in the Bone Marrow of Multiple Myeloma Patients by Transcriptome Analysis

    doi: 10.4274/balkanmedj.2018.0356

    Figure Lengend Snippet: Bone marrow B cell flow cytometry results from a healthy donor. First, B cells were gated by using specific cell surface markers that were CD138 + and CD38 + by determining forward and side light scattering characteristics on the FACSAria II Cell Sorter (Becton Dickinson, San Jose, CA, USA). Then sorted B cells using with cell sorting by the cell surface markers CD56 + , CD19 + according to the FACSAria II Cell Sorter.

    Article Snippet: Fluorescence-activated cell sorting Myeloma cells (CD38+, CD138+, CD19-, and CD56+) and healthy B cells (CD38+, CD138+, CD19+, and CD56-) were selected from bone marrow mononuclear cells using a gating strategy by simultaneously specifying cell surface markers, and by determining forward and side light scattering characteristics on the FACSAria II Cell Sorter (Becton Dickinson, San Jose, CA, USA) ( , ).

    Techniques: Flow Cytometry, Cytometry, FACS

    Flow cytometry results of malignant B cells from bone marrow of a patient with Multiple myeloma. First, myeloma cells were gated by using specific cell surface markers that were CD138 + and CD38 + by determining forward and side light scattering characteristics on the FACSAria II Cell Sorter (Becton Dickinson, San Jose, CA, USA). Then sorted malignant Multiple myeloma cells using with cell sorting by the cell surface markers CD56+, CD19- according to the FACSAria II Cell Sorter.

    Journal: Balkan Medical Journal

    Article Title: Investigation of Gene Expressions of Myeloma Cells in the Bone Marrow of Multiple Myeloma Patients by Transcriptome Analysis

    doi: 10.4274/balkanmedj.2018.0356

    Figure Lengend Snippet: Flow cytometry results of malignant B cells from bone marrow of a patient with Multiple myeloma. First, myeloma cells were gated by using specific cell surface markers that were CD138 + and CD38 + by determining forward and side light scattering characteristics on the FACSAria II Cell Sorter (Becton Dickinson, San Jose, CA, USA). Then sorted malignant Multiple myeloma cells using with cell sorting by the cell surface markers CD56+, CD19- according to the FACSAria II Cell Sorter.

    Article Snippet: Fluorescence-activated cell sorting Myeloma cells (CD38+, CD138+, CD19-, and CD56+) and healthy B cells (CD38+, CD138+, CD19+, and CD56-) were selected from bone marrow mononuclear cells using a gating strategy by simultaneously specifying cell surface markers, and by determining forward and side light scattering characteristics on the FACSAria II Cell Sorter (Becton Dickinson, San Jose, CA, USA) ( , ).

    Techniques: Flow Cytometry, Cytometry, FACS

    Adjuvanticity of MDP, chitosan, Pam 3 CSK 4 , MPL, and iE-DAP to OVA in OVA-specific TCR transgenic mice. Naive OVA-specific T cells were purified from the spleens and inguinal lymph nodes of CD45.1×OTII F 1 mice and transferred into female C57BL/6J mice. One day later, the recipients were immunized via buccal mucosal injection with one of seven compositions in 40 μl, namely, 1 μg of OVA, OVA plus 40 μg of MDP, OVA plus 32 μg of chitosan, OVA plus 25 μg of Pam 3 CSK 4 , OVA plus 15 μg of MPL, OVA plus 50 μg of iE-DAP, or PBS control. Three days later, the mice were sacrificed. The draining submandibular lymph nodes were isolated, and the cells were counted (A). Single-cell suspensions were made for staining with CD45.1 plus CD4 and then run on a BD FACSAria II and analyzed for the number of OVA-specific T cells (B). *, Significantly different from the PBS group (*, P

    Journal: Infection and Immunity

    Article Title: The Combinations Chitosan-Pam3CSK4 and Chitosan-Monophosphoryl Lipid A: Promising Immune-Enhancing Adjuvants for Anticaries Vaccine PAc

    doi: 10.1128/IAI.00651-19

    Figure Lengend Snippet: Adjuvanticity of MDP, chitosan, Pam 3 CSK 4 , MPL, and iE-DAP to OVA in OVA-specific TCR transgenic mice. Naive OVA-specific T cells were purified from the spleens and inguinal lymph nodes of CD45.1×OTII F 1 mice and transferred into female C57BL/6J mice. One day later, the recipients were immunized via buccal mucosal injection with one of seven compositions in 40 μl, namely, 1 μg of OVA, OVA plus 40 μg of MDP, OVA plus 32 μg of chitosan, OVA plus 25 μg of Pam 3 CSK 4 , OVA plus 15 μg of MPL, OVA plus 50 μg of iE-DAP, or PBS control. Three days later, the mice were sacrificed. The draining submandibular lymph nodes were isolated, and the cells were counted (A). Single-cell suspensions were made for staining with CD45.1 plus CD4 and then run on a BD FACSAria II and analyzed for the number of OVA-specific T cells (B). *, Significantly different from the PBS group (*, P

    Article Snippet: The cells were labeled with CD16/32 and a mixture of antibodies (CD45.1-FITC, CD4 Percp/Cy5.5, TCRvα2-APC/Cy7, TCRvβ5-APC, CD62L-PE, and CD25-BV650 [all from eBioscience, San Diego, CA]) sequentially before being sorted with CD4+ MACS beads (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany), and the recovered CD4+ cells were further sorted using a BD FACSAria II (BD Biosciences, San Jose, CA) for CD4+ CD45.1+ TCRvα2+ TCRvβ5+ CD62L+ CD25− fractions.

    Techniques: Transgenic Assay, Mouse Assay, Purification, Injection, Isolation, Staining

    Adjuvanticity of MDP, chitosan, Pam 3 CSK 4 , MPL and iE-DAP to OVA in C57BL/6J mice.The C57BL/6J mice were immunized on day 0 via buccal mucosal injection with 100 μg of OVA, OVA plus 40 μg of MDP, OVA plus 32 μg of chitosan, OVA plus 25 μg of Pam 3 CSK 4 , OVA plus 15 μg of MPL, OVA plus 50 μg of iE-DAP, or PBS and boosted on day 7. On day 14, the sera were collected for measuring OVA-specific IgG1 and IgG2b responses (see panel E). On day 15, the mice were administered with 100 μg of OVA via buccal mucosal injection. On day 17, the mice were sacrificed; the draining submandibular lymph nodes were isolated (A), and the cells were counted (B). (C and D) Single-cell suspensions were made for staining with CD4 plus CD8 and then run on a BD FACSAria II and analyzed for the numbers of CD4 + and CD8 + T cells. C57BL/6J mice were immunized with 100 μg of OVA, OVA plus 32 μg of chitosan, OVA plus chitosan plus 15 μg of MPL, or OVA plus chitosan plus 25 μg of Pam 3 CSK 4 on day 0 and boosted on day 7, all via buccal mucosa injection. On day 14, the mice were sacrificed, and sera were harvested to measure the OVA-specific IgG1 and IgG2b responses (F). *, Significantly different from the PBS group (*, P

    Journal: Infection and Immunity

    Article Title: The Combinations Chitosan-Pam3CSK4 and Chitosan-Monophosphoryl Lipid A: Promising Immune-Enhancing Adjuvants for Anticaries Vaccine PAc

    doi: 10.1128/IAI.00651-19

    Figure Lengend Snippet: Adjuvanticity of MDP, chitosan, Pam 3 CSK 4 , MPL and iE-DAP to OVA in C57BL/6J mice.The C57BL/6J mice were immunized on day 0 via buccal mucosal injection with 100 μg of OVA, OVA plus 40 μg of MDP, OVA plus 32 μg of chitosan, OVA plus 25 μg of Pam 3 CSK 4 , OVA plus 15 μg of MPL, OVA plus 50 μg of iE-DAP, or PBS and boosted on day 7. On day 14, the sera were collected for measuring OVA-specific IgG1 and IgG2b responses (see panel E). On day 15, the mice were administered with 100 μg of OVA via buccal mucosal injection. On day 17, the mice were sacrificed; the draining submandibular lymph nodes were isolated (A), and the cells were counted (B). (C and D) Single-cell suspensions were made for staining with CD4 plus CD8 and then run on a BD FACSAria II and analyzed for the numbers of CD4 + and CD8 + T cells. C57BL/6J mice were immunized with 100 μg of OVA, OVA plus 32 μg of chitosan, OVA plus chitosan plus 15 μg of MPL, or OVA plus chitosan plus 25 μg of Pam 3 CSK 4 on day 0 and boosted on day 7, all via buccal mucosa injection. On day 14, the mice were sacrificed, and sera were harvested to measure the OVA-specific IgG1 and IgG2b responses (F). *, Significantly different from the PBS group (*, P

    Article Snippet: The cells were labeled with CD16/32 and a mixture of antibodies (CD45.1-FITC, CD4 Percp/Cy5.5, TCRvα2-APC/Cy7, TCRvβ5-APC, CD62L-PE, and CD25-BV650 [all from eBioscience, San Diego, CA]) sequentially before being sorted with CD4+ MACS beads (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany), and the recovered CD4+ cells were further sorted using a BD FACSAria II (BD Biosciences, San Jose, CA) for CD4+ CD45.1+ TCRvα2+ TCRvβ5+ CD62L+ CD25− fractions.

    Techniques: Mouse Assay, Injection, Isolation, Staining