Journal: Particle and Fibre Toxicology
Article Title: Nanoparticle-allergen interactions mediate human allergic responses: protein corona characterization and cellular responses
Figure Lengend Snippet: Characterization of AuNPs and AuNP-allergen conjugates. a TEM imaging and TEM negative staining. b Size distribution by intensity obtained from DLS measurements. c Hyperspectral images of AuNPs compared to AuNP-allergen conjugates. d HPLC analysis of allergens before and after the conjugation with AuNPs
Article Snippet: Preparation and characterization of AuNPs and AuNP-allergen conjugates Allergen conjugation was performed using commercially available citrate-stabilized 50 nm AuNPs (Sigma-Aldrich), and characterized using TEM, DLS, and zeta potential determination.
Techniques: Transmission Electron Microscopy, Imaging, Negative Staining, High Performance Liquid Chromatography, Conjugation Assay