f. nucleatum Search Results


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  • 99
    ATCC f nucleatum atcc 25586
    Effects of exogenous indole on planktonic and biofilm cells of F. <t>nucleatum</t> ATCC 25586. (A) Relative amounts of planktonic and biofilm cells grown in the absence or presence of exogenous indole are shown. Bacteria were incubated for 48 h in 96-well PVC
    F Nucleatum Atcc 25586, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 688 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC f nucleatum atcc 23726
    Roles of fusobacterial ftsX and envC in the development of monospecies and multispecies biofilms. (A) ftsX locus in the chromosome of F. <t>nucleatum</t> ATCC 23726, with envC adjacent to ppnK , which encodes an NAD+ kinase. Upstream of ftsX is fadA coding for the adhesin FadA ( 6 ); expression of genes in the ftsX locus and fadA appears to be controlled by individual promoters. (B) Map of the nonreplicative vector pCWU8 used to generate unmarked, in-frame gene deletion mutants in F. nucleatum . The kanamycin resistance cassette ( kan ), chloramphenicol/thiamphenicol resistance gene ( catP ), and galK are indicated. The multiple cloning sites (MCS) contain EcoRI, SacI, KpnI, BamHI, and SalI. (C) Fusobacterial strains were evaluated for their ability to form monospecies biofilm using crystal violet staining. (D) Interaction of fusobacteria and S. oralis So34 was determined by a standard coaggregation assay, with a radD mutant used as a negative control. (E) Three-species biofilms were analyzed by confocal laser scanning microscopy at a magnification of ×20, using 16S rRNA-oligonucleotide probes specific for F. nucleatum (red), A. oris (green), and S. oralis (blue); side and top views are presented. The results are representative of three independent experiments performed in triplicate.
    F Nucleatum Atcc 23726, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 391 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    ATCC f nucleatum atcc 10953
    The effect of the environmental conditions on the activity of H 2 S-producing enzymes was tested for Fusobacterium <t>nucleatum</t> polymorphum ATCC 10953. The bacteria were grown in different broths before protein extraction and 1D gel electrophoresis followed by in-gel activity assay
    F Nucleatum Atcc 10953, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 244 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    ATCC f nucleatum atcc 49256
    Amino acid sequence alignment of FadA and its paralogues. Highlighted in gray are the identical residues shared among FadA proteins. The sequences of two FadA paralogues, FN1529 from F. <t>nucleatum</t> ATCC 25586 and FNV2159 from F. nucleatum ATCC 49256, are
    F Nucleatum Atcc 49256, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Cancer Therapeutics CRC f nucleatum
    Interactions between F. <t>nucleatum</t> and epithelial cells that could produce an oncogenic phenotype. Binding of the FadA adhesin to E-cadherin activates β-catenin signaling, resulting in activation of genes that control cell survival and proliferation. F. nucleatum also activates several cyclin dependent kinases (CDKs) and p38, which controls the production of matrix metalloproteases MMP-9 and MMP-13.
    F Nucleatum, supplied by Cancer Therapeutics CRC, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    ATCC f nucleatum 12230
    Growth of F. <t>nucleatum</t> 12230 (solid triangles and solid line) and F. nucleatum 12230-US1 (open squares and dashed line) in Columbia broth. OD 600, optical density at 600 nm.
    F Nucleatum 12230, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    ATCC f nucleatum atcc 25286
    Growth of F. <t>nucleatum</t> 12230 (solid triangles and solid line) and F. nucleatum 12230-US1 (open squares and dashed line) in Columbia broth. OD 600, optical density at 600 nm.
    F Nucleatum Atcc 25286, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Envigo wt f nucleatum
    Growth of F. <t>nucleatum</t> 12230 (solid triangles and solid line) and F. nucleatum 12230-US1 (open squares and dashed line) in Columbia broth. OD 600, optical density at 600 nm.
    Wt F Nucleatum, supplied by Envigo, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher gene encoding f nucleatum nank
    2.1. F. <t>nucleatum</t> <t>NanK</t> production
    Gene Encoding F Nucleatum Nank, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Pacific Immunology rabbit anti f nucleatum polyclonal antibody
    2.1. F. <t>nucleatum</t> <t>NanK</t> production
    Rabbit Anti F Nucleatum Polyclonal Antibody, supplied by Pacific Immunology, used in various techniques. Bioz Stars score: 90/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    ATCC f nucleatum atcc 10596
    2.1. F. <t>nucleatum</t> <t>NanK</t> production
    F Nucleatum Atcc 10596, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    ATCC f nucleatum atcc 23276 competent cells
    2.1. F. <t>nucleatum</t> <t>NanK</t> production
    F Nucleatum Atcc 23276 Competent Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    ATCC f nucleatum atcc 23727 attachment
    2.1. F. <t>nucleatum</t> <t>NanK</t> production
    F Nucleatum Atcc 23727 Attachment, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effects of exogenous indole on planktonic and biofilm cells of F. nucleatum ATCC 25586. (A) Relative amounts of planktonic and biofilm cells grown in the absence or presence of exogenous indole are shown. Bacteria were incubated for 48 h in 96-well PVC

    Journal: Applied and Environmental Microbiology

    Article Title: Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿

    doi: 10.1128/AEM.00166-10

    Figure Lengend Snippet: Effects of exogenous indole on planktonic and biofilm cells of F. nucleatum ATCC 25586. (A) Relative amounts of planktonic and biofilm cells grown in the absence or presence of exogenous indole are shown. Bacteria were incubated for 48 h in 96-well PVC

    Article Snippet: However, the transcription start site of the tna operon in F. nucleatum ATCC 25586 was 67 or 152 bp upstream from the initiation codon of tnaA (Fig. ), revealing that the region corresponding to tnaL in F. nucleatum was much shorter than that in E. coli.

    Techniques: Incubation

    Characterization of purified protein encoded by fn1943 of F. nucleatum ATCC 25586. (A) SDS-PAGE analysis of recombinant Fn1943 of F. nucleatum ATCC 25586. Proteins were purified by affinity chromatography on glutathione-Sepharose 4B resin and digested

    Journal: Applied and Environmental Microbiology

    Article Title: Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿

    doi: 10.1128/AEM.00166-10

    Figure Lengend Snippet: Characterization of purified protein encoded by fn1943 of F. nucleatum ATCC 25586. (A) SDS-PAGE analysis of recombinant Fn1943 of F. nucleatum ATCC 25586. Proteins were purified by affinity chromatography on glutathione-Sepharose 4B resin and digested

    Article Snippet: However, the transcription start site of the tna operon in F. nucleatum ATCC 25586 was 67 or 152 bp upstream from the initiation codon of tnaA (Fig. ), revealing that the region corresponding to tnaL in F. nucleatum was much shorter than that in E. coli.

    Techniques: Purification, SDS Page, Recombinant, Affinity Chromatography

    Genetic organization and transcriptional analyses of the tnaA region of F. nucleatum ATCC 25586. (A) Gene arrangements of the tnaA region in F. nucleatum ATCC 25586, E. coli K-12, and P. gingivalis W83. The DNA sequence of each region was obtained from

    Journal: Applied and Environmental Microbiology

    Article Title: Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿

    doi: 10.1128/AEM.00166-10

    Figure Lengend Snippet: Genetic organization and transcriptional analyses of the tnaA region of F. nucleatum ATCC 25586. (A) Gene arrangements of the tnaA region in F. nucleatum ATCC 25586, E. coli K-12, and P. gingivalis W83. The DNA sequence of each region was obtained from

    Article Snippet: However, the transcription start site of the tna operon in F. nucleatum ATCC 25586 was 67 or 152 bp upstream from the initiation codon of tnaA (Fig. ), revealing that the region corresponding to tnaL in F. nucleatum was much shorter than that in E. coli.

    Techniques: Sequencing

    Expression levels of tnaA in several cell growth phases of F. nucleatum ATCC 25586. The OD 595 of cells incubated for 4, 8, 12, or 24 h for RNA extraction is indicated as an inset. The amounts of tnaA cDNA in each sample were analyzed by real-time quantitative

    Journal: Applied and Environmental Microbiology

    Article Title: Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿

    doi: 10.1128/AEM.00166-10

    Figure Lengend Snippet: Expression levels of tnaA in several cell growth phases of F. nucleatum ATCC 25586. The OD 595 of cells incubated for 4, 8, 12, or 24 h for RNA extraction is indicated as an inset. The amounts of tnaA cDNA in each sample were analyzed by real-time quantitative

    Article Snippet: However, the transcription start site of the tna operon in F. nucleatum ATCC 25586 was 67 or 152 bp upstream from the initiation codon of tnaA (Fig. ), revealing that the region corresponding to tnaL in F. nucleatum was much shorter than that in E. coli.

    Techniques: Expressing, Incubation, RNA Extraction

    Effects of tryptophan on indole production and biofilm formation by F. nucleatum ATCC 25586. (A) Indole production by F. nucleatum ATCC 25586 cells grown in the absence or presence of tryptophan; (B) relative amounts of planktonic and biofilm cells grown

    Journal: Applied and Environmental Microbiology

    Article Title: Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿

    doi: 10.1128/AEM.00166-10

    Figure Lengend Snippet: Effects of tryptophan on indole production and biofilm formation by F. nucleatum ATCC 25586. (A) Indole production by F. nucleatum ATCC 25586 cells grown in the absence or presence of tryptophan; (B) relative amounts of planktonic and biofilm cells grown

    Article Snippet: However, the transcription start site of the tna operon in F. nucleatum ATCC 25586 was 67 or 152 bp upstream from the initiation codon of tnaA (Fig. ), revealing that the region corresponding to tnaL in F. nucleatum was much shorter than that in E. coli.

    Techniques:

    (A) Western immunoblot of FAD-I protein in whole-cell lysates of F. nucleatum ATCC 25586, ATCC 23726, ATCC 10953, ATCC 10953 Δ fadI , and ATCC 10953 Δ fadI /pFAD-I 23726 (ATCC 10953 Δ fadI with plasmid-based expression of FAD-I from

    Journal: Infection and Immunity

    Article Title: FAD-I, a Fusobacterium nucleatum Cell Wall-Associated Diacylated Lipoprotein That Mediates Human Beta Defensin 2 Induction through Toll-Like Receptor-1/2 (TLR-1/2) and TLR-2/6

    doi: 10.1128/IAI.01311-15

    Figure Lengend Snippet: (A) Western immunoblot of FAD-I protein in whole-cell lysates of F. nucleatum ATCC 25586, ATCC 23726, ATCC 10953, ATCC 10953 Δ fadI , and ATCC 10953 Δ fadI /pFAD-I 23726 (ATCC 10953 Δ fadI with plasmid-based expression of FAD-I from

    Article Snippet: The full-length FAD-I gene (FN1527) ( ) was amplified by PCR from genomic DNA of F. nucleatum strain ATCC 25586 using primers (see Table S2 in the supplemental material) with Nde1 and Xho1 restriction sites at their ends (New England BioLabs).

    Techniques: Western Blot, Plasmid Preparation, Expressing

    Induction of hBD-2 mRNA by live bacterial cells (A) and cell wall preparations (10 μg/ml cell wall F. nucleatum [Fn] ATCC 25586, ATCC 23726, and ATCC 10953 (B). The data presented are means ± standard deviations (SD) of the results of

    Journal: Infection and Immunity

    Article Title: FAD-I, a Fusobacterium nucleatum Cell Wall-Associated Diacylated Lipoprotein That Mediates Human Beta Defensin 2 Induction through Toll-Like Receptor-1/2 (TLR-1/2) and TLR-2/6

    doi: 10.1128/IAI.01311-15

    Figure Lengend Snippet: Induction of hBD-2 mRNA by live bacterial cells (A) and cell wall preparations (10 μg/ml cell wall F. nucleatum [Fn] ATCC 25586, ATCC 23726, and ATCC 10953 (B). The data presented are means ± standard deviations (SD) of the results of

    Article Snippet: The full-length FAD-I gene (FN1527) ( ) was amplified by PCR from genomic DNA of F. nucleatum strain ATCC 25586 using primers (see Table S2 in the supplemental material) with Nde1 and Xho1 restriction sites at their ends (New England BioLabs).

    Techniques:

    Roles of fusobacterial ftsX and envC in the development of monospecies and multispecies biofilms. (A) ftsX locus in the chromosome of F. nucleatum ATCC 23726, with envC adjacent to ppnK , which encodes an NAD+ kinase. Upstream of ftsX is fadA coding for the adhesin FadA ( 6 ); expression of genes in the ftsX locus and fadA appears to be controlled by individual promoters. (B) Map of the nonreplicative vector pCWU8 used to generate unmarked, in-frame gene deletion mutants in F. nucleatum . The kanamycin resistance cassette ( kan ), chloramphenicol/thiamphenicol resistance gene ( catP ), and galK are indicated. The multiple cloning sites (MCS) contain EcoRI, SacI, KpnI, BamHI, and SalI. (C) Fusobacterial strains were evaluated for their ability to form monospecies biofilm using crystal violet staining. (D) Interaction of fusobacteria and S. oralis So34 was determined by a standard coaggregation assay, with a radD mutant used as a negative control. (E) Three-species biofilms were analyzed by confocal laser scanning microscopy at a magnification of ×20, using 16S rRNA-oligonucleotide probes specific for F. nucleatum (red), A. oris (green), and S. oralis (blue); side and top views are presented. The results are representative of three independent experiments performed in triplicate.

    Journal: mBio

    Article Title: Forward Genetic Dissection of Biofilm Development by Fusobacterium nucleatum: Novel Functions of Cell Division Proteins FtsX and EnvC

    doi: 10.1128/mBio.00360-18

    Figure Lengend Snippet: Roles of fusobacterial ftsX and envC in the development of monospecies and multispecies biofilms. (A) ftsX locus in the chromosome of F. nucleatum ATCC 23726, with envC adjacent to ppnK , which encodes an NAD+ kinase. Upstream of ftsX is fadA coding for the adhesin FadA ( 6 ); expression of genes in the ftsX locus and fadA appears to be controlled by individual promoters. (B) Map of the nonreplicative vector pCWU8 used to generate unmarked, in-frame gene deletion mutants in F. nucleatum . The kanamycin resistance cassette ( kan ), chloramphenicol/thiamphenicol resistance gene ( catP ), and galK are indicated. The multiple cloning sites (MCS) contain EcoRI, SacI, KpnI, BamHI, and SalI. (C) Fusobacterial strains were evaluated for their ability to form monospecies biofilm using crystal violet staining. (D) Interaction of fusobacteria and S. oralis So34 was determined by a standard coaggregation assay, with a radD mutant used as a negative control. (E) Three-species biofilms were analyzed by confocal laser scanning microscopy at a magnification of ×20, using 16S rRNA-oligonucleotide probes specific for F. nucleatum (red), A. oris (green), and S. oralis (blue); side and top views are presented. The results are representative of three independent experiments performed in triplicate.

    Article Snippet: To construct this vector, primer sets PcatP-F/R and com-FtsX-F/R ( ) were used to amplify the catP promoter from plasmid pHS30 and the ftsX coding region from the genomic DNA of F. nucleatum ATCC 23726, appending SacI/KpnI or KpnI/XhoI sites for cloning purposes, respectively.

    Techniques: Expressing, Plasmid Preparation, Clone Assay, Staining, Mutagenesis, Negative Control, Confocal Laser Scanning Microscopy

    Desulfobulbus sp. strain HOT041 ( D. oralis ) in coculture with Fusobacterium nucleatum and in pure culture. (A) FISH using fluorescent oligonucleotide probes specific for Deltaproteobacteria (green) and universal Bacteria (red). (B). Growth of Desulfobulbus sp. strain HOT041 and F. nucleatum in coculture monitored by species-specific qPCR (with error bars based on three replicates). (C and D) Scanning electron micrographs of the D. oralis isolate. The arrowheads point to membrane vesicles.

    Journal: mBio

    Article Title: Insights into the Evolution of Host Association through the Isolation and Characterization of a Novel Human Periodontal Pathobiont, Desulfobulbus oralis

    doi: 10.1128/mBio.02061-17

    Figure Lengend Snippet: Desulfobulbus sp. strain HOT041 ( D. oralis ) in coculture with Fusobacterium nucleatum and in pure culture. (A) FISH using fluorescent oligonucleotide probes specific for Deltaproteobacteria (green) and universal Bacteria (red). (B). Growth of Desulfobulbus sp. strain HOT041 and F. nucleatum in coculture monitored by species-specific qPCR (with error bars based on three replicates). (C and D) Scanning electron micrographs of the D. oralis isolate. The arrowheads point to membrane vesicles.

    Article Snippet: CFS from a type strain of F. nucleatum (ATCC 23726) also supported the growth of D. oralis , indicating its physiological dependence is not restricted to the strain it coenriched with, although we have not extended that analysis to additional strains or species.

    Techniques: Fluorescence In Situ Hybridization, Real-time Polymerase Chain Reaction

    Identification of Fap2 Homologs in F. nucleatum ATCC 23726

    Journal: Journal of dental research

    Article Title: Fusobacterium nucleatum Apoptosis-inducing Outer Membrane Protein

    doi:

    Figure Lengend Snippet: Identification of Fap2 Homologs in F. nucleatum ATCC 23726

    Article Snippet: In this study, we identified homologs to the apoptosis-inducing protein Fap2 in the transformable F. nucleatum strain ATCC 23726, created an isogenic mutant for the aim 1 gene, and demonstrated that the mutation impaired the ability of F. nucleatum to induce apoptosis.

    Techniques:

    Structural and transcriptional analyses of wild-type and aim less1 mutant strains. (A) Schematic illustration of mutagenesis: organization of aim 1 and flanking genes on the F. nucleatum ATCC 23726 wild-type and aim less1 mutant chromosome. Arrows indicate

    Journal: Journal of dental research

    Article Title: Fusobacterium nucleatum Apoptosis-inducing Outer Membrane Protein

    doi:

    Figure Lengend Snippet: Structural and transcriptional analyses of wild-type and aim less1 mutant strains. (A) Schematic illustration of mutagenesis: organization of aim 1 and flanking genes on the F. nucleatum ATCC 23726 wild-type and aim less1 mutant chromosome. Arrows indicate

    Article Snippet: In this study, we identified homologs to the apoptosis-inducing protein Fap2 in the transformable F. nucleatum strain ATCC 23726, created an isogenic mutant for the aim 1 gene, and demonstrated that the mutation impaired the ability of F. nucleatum to induce apoptosis.

    Techniques: Mutagenesis

    F. nucleatum ATCC 23726 accelerates tumor growth and progression of lung metastasis. a Experimental scheme: AT3 breast cancer cell line was injected to the mammary fat pad of 6–7-week-old C57BL/6 mice. When tumor size reached 500 mm 3 , PBS vehicle (V), 5 × 10 7 F. nucleatum ATCC 23726 only (726), 5 × 10 7 F. nucleatum ATCC 23726 with metronidazole (726+MTZ.), metronidazole only (MTZ.), or 5 × 10 7 Fap2-deficient F. nucleatum K50 were injected into the tail vein. On days 2–3 post inoculation, mice were injected twice a day with metronidazole (M) or with PBS vehicle (V). On days 4–7 post inoculation, mice were injected once a day with metronidazole or with PBS. Eight days after inoculation, mice were sacrificed, and tumor and lungs harvested. Breast cancer tissues were weighed, and lung metastases were counted. b Tumor weights normalized as the percentage compared with the average tumor weight in the PBS group (vehicle control) in each experiment (set as 100% tumor weight). Each symbol represents one mouse. Presented results are of three independent experiments. **** p = 3.48 × 10 −5 , ** p = 0.002. Holm’s correction, one-tailed Mann–Whitney. Vehicle n = 21, MTZ. n = 17, K50 n = 7, 726 n = 19, 726+MTZ. n = 12. c Representative tumors post-harvest. d Representative image of lung metastases. Scale bar, 500 µm in the large image, 250 µm in the indicated inset. e Lung metastasis rank [number of metastases per lung area (pixel 2 )] calculated as percentage compared with the average metastasis rank in the vehicle control group of each experiment (set as 100% metastasis rank). Each symbol represents one mouse. Presented results are of three independent experiments. **** p = 4.08 × 10 −5 , * p = 0.0177, n.s.: non-significant, Holm’s correction, one-tailed Mann–Whitney. Vehicle n = 20, MTZ. n = 17, K50 n = 7, 726 n = 18, 726+met n = 12. f Images taken by fluorescence binocular of lung metastases in four mice implanted with GFP-expressing AT3 cells as described above. Two tumor-bearing mice were inoculated with F. nucleatum and two were treated by PBS vehicle. Scale bar, 1000 µm. Source data are provided as a Source data file.

    Journal: Nature Communications

    Article Title: Breast cancer colonization by Fusobacterium nucleatum accelerates tumor growth and metastatic progression

    doi: 10.1038/s41467-020-16967-2

    Figure Lengend Snippet: F. nucleatum ATCC 23726 accelerates tumor growth and progression of lung metastasis. a Experimental scheme: AT3 breast cancer cell line was injected to the mammary fat pad of 6–7-week-old C57BL/6 mice. When tumor size reached 500 mm 3 , PBS vehicle (V), 5 × 10 7 F. nucleatum ATCC 23726 only (726), 5 × 10 7 F. nucleatum ATCC 23726 with metronidazole (726+MTZ.), metronidazole only (MTZ.), or 5 × 10 7 Fap2-deficient F. nucleatum K50 were injected into the tail vein. On days 2–3 post inoculation, mice were injected twice a day with metronidazole (M) or with PBS vehicle (V). On days 4–7 post inoculation, mice were injected once a day with metronidazole or with PBS. Eight days after inoculation, mice were sacrificed, and tumor and lungs harvested. Breast cancer tissues were weighed, and lung metastases were counted. b Tumor weights normalized as the percentage compared with the average tumor weight in the PBS group (vehicle control) in each experiment (set as 100% tumor weight). Each symbol represents one mouse. Presented results are of three independent experiments. **** p = 3.48 × 10 −5 , ** p = 0.002. Holm’s correction, one-tailed Mann–Whitney. Vehicle n = 21, MTZ. n = 17, K50 n = 7, 726 n = 19, 726+MTZ. n = 12. c Representative tumors post-harvest. d Representative image of lung metastases. Scale bar, 500 µm in the large image, 250 µm in the indicated inset. e Lung metastasis rank [number of metastases per lung area (pixel 2 )] calculated as percentage compared with the average metastasis rank in the vehicle control group of each experiment (set as 100% metastasis rank). Each symbol represents one mouse. Presented results are of three independent experiments. **** p = 4.08 × 10 −5 , * p = 0.0177, n.s.: non-significant, Holm’s correction, one-tailed Mann–Whitney. Vehicle n = 20, MTZ. n = 17, K50 n = 7, 726 n = 18, 726+met n = 12. f Images taken by fluorescence binocular of lung metastases in four mice implanted with GFP-expressing AT3 cells as described above. Two tumor-bearing mice were inoculated with F. nucleatum and two were treated by PBS vehicle. Scale bar, 1000 µm. Source data are provided as a Source data file.

    Article Snippet: Supporting the predicted Fap2-mediated recognition of Gal-GalNAc, wild-type F. nucleatum ATCC 23726 showed significantly higher attachment than the K50 and D22 mutants (Fig. ).

    Techniques: Injection, Mouse Assay, One-tailed Test, MANN-WHITNEY, Fluorescence, Expressing

    AT3 mammary tumor acceleration by F. nucleatum is immune-mediated. a 1 × 10 6 AT3 cells were injected to the mammary fat pad of 6–7-week-old female C57BL/6 mice. When tumor volume reached 500 mm 3 , mice were IV injected with PBS vehicle or with 5 × 10 7 F. nucleatum ATCC 23726 (726). At day 8 post injection, mice were sacrificed, tumors were harvested subjected to flow cytometry and abundance of NK cells, CD4+ T cells and CD8+ T cells was determined. Boxplot whiskers represent extrema, box bounds represent upper and lower quartiles, and center-line represents the median value. n.s.: non-significant. ** p = 0.00216, * p = 0.0152, two-tailed Mann–Whitney (each symbol represents the mean of two technical replicates, n = 6 per group). b 1 × 10 5 AT3 or GFP-expressing AT3 cells were injected to the mammary fat pad of 6–7-week-old female SCID-beige mice. When tumor volume reached 500 mm 3 , mice were IV injected with PBS vehicle or with 5 × 10 7 F. nucleatum ATCC 23726 (726). At day 8 post injection, mice were sacrificed, tumors were harvested and weighed in an analytic weigh. Boxplot whiskers represent extrema, box bounds represent upper and lower quartiles, and center-line represents the median value of two independent experiments. n.s.: non-significant. n = 8 per vehicle group and n = 10 per F. nucleatum -infected group. c Representative picture and densitometry analysis of gelatin zymography of conditioned medium prepared from mouse mammary tumor cell line AT3 or from the human breast cancer cell line MDA-MB-231 that were co-cultured or not, with F. nucleatum strains ATCC 25586 or ATCC 23726. Bars indicate mean ± SEM of six independent experiments. * p = 0.031 two-tailed paired Wilcoxon. Note that mouse MMP-9 is larger than that of human. Source data are provided as a Source data file.

    Journal: Nature Communications

    Article Title: Breast cancer colonization by Fusobacterium nucleatum accelerates tumor growth and metastatic progression

    doi: 10.1038/s41467-020-16967-2

    Figure Lengend Snippet: AT3 mammary tumor acceleration by F. nucleatum is immune-mediated. a 1 × 10 6 AT3 cells were injected to the mammary fat pad of 6–7-week-old female C57BL/6 mice. When tumor volume reached 500 mm 3 , mice were IV injected with PBS vehicle or with 5 × 10 7 F. nucleatum ATCC 23726 (726). At day 8 post injection, mice were sacrificed, tumors were harvested subjected to flow cytometry and abundance of NK cells, CD4+ T cells and CD8+ T cells was determined. Boxplot whiskers represent extrema, box bounds represent upper and lower quartiles, and center-line represents the median value. n.s.: non-significant. ** p = 0.00216, * p = 0.0152, two-tailed Mann–Whitney (each symbol represents the mean of two technical replicates, n = 6 per group). b 1 × 10 5 AT3 or GFP-expressing AT3 cells were injected to the mammary fat pad of 6–7-week-old female SCID-beige mice. When tumor volume reached 500 mm 3 , mice were IV injected with PBS vehicle or with 5 × 10 7 F. nucleatum ATCC 23726 (726). At day 8 post injection, mice were sacrificed, tumors were harvested and weighed in an analytic weigh. Boxplot whiskers represent extrema, box bounds represent upper and lower quartiles, and center-line represents the median value of two independent experiments. n.s.: non-significant. n = 8 per vehicle group and n = 10 per F. nucleatum -infected group. c Representative picture and densitometry analysis of gelatin zymography of conditioned medium prepared from mouse mammary tumor cell line AT3 or from the human breast cancer cell line MDA-MB-231 that were co-cultured or not, with F. nucleatum strains ATCC 25586 or ATCC 23726. Bars indicate mean ± SEM of six independent experiments. * p = 0.031 two-tailed paired Wilcoxon. Note that mouse MMP-9 is larger than that of human. Source data are provided as a Source data file.

    Article Snippet: Supporting the predicted Fap2-mediated recognition of Gal-GalNAc, wild-type F. nucleatum ATCC 23726 showed significantly higher attachment than the K50 and D22 mutants (Fig. ).

    Techniques: Injection, Mouse Assay, Flow Cytometry, Two Tailed Test, MANN-WHITNEY, Expressing, Infection, Zymography, Multiple Displacement Amplification, Cell Culture

    F. nucleatum colonizes mammary cancer in a Fap2-mediated mechanism. a Orthotropic 4T1 BALB/c mouse mammary cancer model. When reaching 500 mm 3 size, 5 × 10 7 F. nucleatum ATCC 23726 were IV injected. Twenty-four hours post inoculation, breast cancer and normal tissue from an adjacent mammary were harvested. b Representative images and sum of fluorescence intensity of binding of FITC-labeled PNA (green) to tumor and normal tissue stained with Hoechst dye (blue). Each paired symbols represents one mouse ( n = 7), ** p = 0.0078, one-tailed, Wilcoxon matched-paired signed rank test. c Relative F. nucleatum gDNA abundance (2 −ΔCt ) in breast tissue from tumor-free mice (No tumor, n = 3), and in tumor and matching normal tissue of 4T1 tumor-bearing mice ( n = 5) all inoculated with F. nucleatum ATCC 23726. * p = 0.0358 Holm-corrected one-tailed Mann–Whitney test. * p = 0.0313, one-tailed, Wilcoxon matched-paired signed rank test (for paired normal adjacent-tumor samples). d Orthotropic AT3 C57BL/6 mouse mammary cancer model. When reaching 500 mm 3 , mice were inoculated with 5 × 10 7 F. nucleatum ATCC 23726 (726; blue), 5 × 10 7 Fap2 mutant K50 (MUT K50; red) or with 5 × 10 7 P. gingivalis (Pg; black). Twenty-four hours later, normal mammary and breast cancer tissue were harvested from each mice and bacteria quantified. e Bacterial abundance (CFU/g tissue), and relative bacterial gDNA abundance (2 −ΔCt ) in tumor and normal tissue of AT3 tumor-bearing mice inoculated with P. gingivalis ( Pg , n = 9), K50 ( n = 12), or F. nucleatum ATCC 23726 (726, n = 12). Each symbol represents one mouse. * p = 0.0156 one-tailed, Wilcoxon matched-paired signed rank test. *** p = 0.0006, ** p = 0.001598. Holm-corrected one-tailed Mann–Whitney test (left panel). **** p

    Journal: Nature Communications

    Article Title: Breast cancer colonization by Fusobacterium nucleatum accelerates tumor growth and metastatic progression

    doi: 10.1038/s41467-020-16967-2

    Figure Lengend Snippet: F. nucleatum colonizes mammary cancer in a Fap2-mediated mechanism. a Orthotropic 4T1 BALB/c mouse mammary cancer model. When reaching 500 mm 3 size, 5 × 10 7 F. nucleatum ATCC 23726 were IV injected. Twenty-four hours post inoculation, breast cancer and normal tissue from an adjacent mammary were harvested. b Representative images and sum of fluorescence intensity of binding of FITC-labeled PNA (green) to tumor and normal tissue stained with Hoechst dye (blue). Each paired symbols represents one mouse ( n = 7), ** p = 0.0078, one-tailed, Wilcoxon matched-paired signed rank test. c Relative F. nucleatum gDNA abundance (2 −ΔCt ) in breast tissue from tumor-free mice (No tumor, n = 3), and in tumor and matching normal tissue of 4T1 tumor-bearing mice ( n = 5) all inoculated with F. nucleatum ATCC 23726. * p = 0.0358 Holm-corrected one-tailed Mann–Whitney test. * p = 0.0313, one-tailed, Wilcoxon matched-paired signed rank test (for paired normal adjacent-tumor samples). d Orthotropic AT3 C57BL/6 mouse mammary cancer model. When reaching 500 mm 3 , mice were inoculated with 5 × 10 7 F. nucleatum ATCC 23726 (726; blue), 5 × 10 7 Fap2 mutant K50 (MUT K50; red) or with 5 × 10 7 P. gingivalis (Pg; black). Twenty-four hours later, normal mammary and breast cancer tissue were harvested from each mice and bacteria quantified. e Bacterial abundance (CFU/g tissue), and relative bacterial gDNA abundance (2 −ΔCt ) in tumor and normal tissue of AT3 tumor-bearing mice inoculated with P. gingivalis ( Pg , n = 9), K50 ( n = 12), or F. nucleatum ATCC 23726 (726, n = 12). Each symbol represents one mouse. * p = 0.0156 one-tailed, Wilcoxon matched-paired signed rank test. *** p = 0.0006, ** p = 0.001598. Holm-corrected one-tailed Mann–Whitney test (left panel). **** p

    Article Snippet: Supporting the predicted Fap2-mediated recognition of Gal-GalNAc, wild-type F. nucleatum ATCC 23726 showed significantly higher attachment than the K50 and D22 mutants (Fig. ).

    Techniques: Injection, Fluorescence, Binding Assay, Labeling, Staining, One-tailed Test, Mouse Assay, MANN-WHITNEY, Mutagenesis

    Attachment of F. nucleatum ATCC 23726 to breast cancer is Fap2-mediated and Gal-GalNAc dependent. a Representative images (left panels, scale bar 20 µm) and quantitative analysis (Fn/mm 2 ) (right panel) of binding of Cy3-labeled (white) WT F. nucleatum ATCC 23726 (726) and of its Cy5-labeled (red) Fap2-inactivated isogenic mutant K50, to Hoechst-stained (blue) human breast cancer TMAs (HBre-Duc060CS-01 and BR1006). Results in the right panel are classified by tissue type (normal n = 35, benign n = 7, malignant n = 64) and bacterial strain (K50, 726). Each core is measured twice, once for each of the strains in the appropriate tissue type (as demonstrated in the pictures). When an individual contributed a single core to the experiment, the two observations are shown as circles (●); when she contributed two cores, these were to the normal type and the malignant type, and the four observations are depicted as plus signs (+). The six classes were compared against each other in pairs: the exact two-tailed Wilcoxon test was used for pairs that were from the same core, the exact two-tailed Mann–Whitney test for independent samples. Where the same hypothesis was tested on more than one pair, and the individual pairs were mutually independent, the corresponding p -values were combined by Fisher’s method. Wherever the pairs were not independent, the multiple comparisons were adjusted for using the Holm modification of the Bonferroni correction. **** p

    Journal: Nature Communications

    Article Title: Breast cancer colonization by Fusobacterium nucleatum accelerates tumor growth and metastatic progression

    doi: 10.1038/s41467-020-16967-2

    Figure Lengend Snippet: Attachment of F. nucleatum ATCC 23726 to breast cancer is Fap2-mediated and Gal-GalNAc dependent. a Representative images (left panels, scale bar 20 µm) and quantitative analysis (Fn/mm 2 ) (right panel) of binding of Cy3-labeled (white) WT F. nucleatum ATCC 23726 (726) and of its Cy5-labeled (red) Fap2-inactivated isogenic mutant K50, to Hoechst-stained (blue) human breast cancer TMAs (HBre-Duc060CS-01 and BR1006). Results in the right panel are classified by tissue type (normal n = 35, benign n = 7, malignant n = 64) and bacterial strain (K50, 726). Each core is measured twice, once for each of the strains in the appropriate tissue type (as demonstrated in the pictures). When an individual contributed a single core to the experiment, the two observations are shown as circles (●); when she contributed two cores, these were to the normal type and the malignant type, and the four observations are depicted as plus signs (+). The six classes were compared against each other in pairs: the exact two-tailed Wilcoxon test was used for pairs that were from the same core, the exact two-tailed Mann–Whitney test for independent samples. Where the same hypothesis was tested on more than one pair, and the individual pairs were mutually independent, the corresponding p -values were combined by Fisher’s method. Wherever the pairs were not independent, the multiple comparisons were adjusted for using the Holm modification of the Bonferroni correction. **** p

    Article Snippet: Supporting the predicted Fap2-mediated recognition of Gal-GalNAc, wild-type F. nucleatum ATCC 23726 showed significantly higher attachment than the K50 and D22 mutants (Fig. ).

    Techniques: Binding Assay, Labeling, Mutagenesis, Staining, Two Tailed Test, MANN-WHITNEY, Modification

    The effect of the environmental conditions on the activity of H 2 S-producing enzymes was tested for Fusobacterium nucleatum polymorphum ATCC 10953. The bacteria were grown in different broths before protein extraction and 1D gel electrophoresis followed by in-gel activity assay

    Journal: BMC Microbiology

    Article Title: The proteins of Fusobacterium spp. involved in hydrogen sulfide production from L-cysteine

    doi: 10.1186/s12866-017-0967-9

    Figure Lengend Snippet: The effect of the environmental conditions on the activity of H 2 S-producing enzymes was tested for Fusobacterium nucleatum polymorphum ATCC 10953. The bacteria were grown in different broths before protein extraction and 1D gel electrophoresis followed by in-gel activity assay

    Article Snippet: For F. nucleatum ATCC 10953, the influence of other environmental conditions were tested in TH broth buffered to pH 6, pH 7, pH 8 or TH broth, pH 7.8 supplemented with glutathione (2.5 mg/ml), sodium sulfide (0.46 mg/ml), 5% serum, 50% serum or 50% serum with hemin (10 ml/l).

    Techniques: Activity Assay, Protein Extraction, Nucleic Acid Electrophoresis

    Amino acid sequence alignment of FadA and its paralogues. Highlighted in gray are the identical residues shared among FadA proteins. The sequences of two FadA paralogues, FN1529 from F. nucleatum ATCC 25586 and FNV2159 from F. nucleatum ATCC 49256, are

    Journal: Journal of Bacteriology

    Article Title: Identification and Characterization of a Novel Adhesin Unique to Oral Fusobacteria

    doi: 10.1128/JB.187.15.5330-5340.2005

    Figure Lengend Snippet: Amino acid sequence alignment of FadA and its paralogues. Highlighted in gray are the identical residues shared among FadA proteins. The sequences of two FadA paralogues, FN1529 from F. nucleatum ATCC 25586 and FNV2159 from F. nucleatum ATCC 49256, are

    Article Snippet: The accession numbers for the FadA sequences from other fusobacterial strains and species are as follows: for F. nucleatum ATCC 10953 , for F. nucleatum ATCC 23726 , for F. nucleatum ATCC 25586 , for F. nucleatum ATCC 49256 , for F. nucleatum ATCC 51190 , for F. nucleatum DUMC1356 , for F. nucleatum DUMC2079 , for F. nucleatum DUMC2929 , for F. nucleatum DUMC3156 , for F. nucleatum DUMC3349 , for F. nucleatum PK1594 , for F. periodonticum ATCC 33693 , and for F. simiae ATCC 33568 .

    Techniques: Sequencing

    Interactions between F. nucleatum and epithelial cells that could produce an oncogenic phenotype. Binding of the FadA adhesin to E-cadherin activates β-catenin signaling, resulting in activation of genes that control cell survival and proliferation. F. nucleatum also activates several cyclin dependent kinases (CDKs) and p38, which controls the production of matrix metalloproteases MMP-9 and MMP-13.

    Journal: PLoS Pathogens

    Article Title: Oral Bacteria and Cancer

    doi: 10.1371/journal.ppat.1003933

    Figure Lengend Snippet: Interactions between F. nucleatum and epithelial cells that could produce an oncogenic phenotype. Binding of the FadA adhesin to E-cadherin activates β-catenin signaling, resulting in activation of genes that control cell survival and proliferation. F. nucleatum also activates several cyclin dependent kinases (CDKs) and p38, which controls the production of matrix metalloproteases MMP-9 and MMP-13.

    Article Snippet: F. nucleatum was found to be one of the more abundant species within and around CRC neoplasms, and levels of F. nucleatum correlated with the presence of lymph node metastases.

    Techniques: Binding Assay, Activation Assay

    Growth of F. nucleatum 12230 (solid triangles and solid line) and F. nucleatum 12230-US1 (open squares and dashed line) in Columbia broth. OD 600, optical density at 600 nm.

    Journal: Journal of Bacteriology

    Article Title: Identification and Characterization of a Novel Adhesin Unique to Oral Fusobacteria

    doi: 10.1128/JB.187.15.5330-5340.2005

    Figure Lengend Snippet: Growth of F. nucleatum 12230 (solid triangles and solid line) and F. nucleatum 12230-US1 (open squares and dashed line) in Columbia broth. OD 600, optical density at 600 nm.

    Article Snippet: Following unsuccessful attempts to generate a correct fadA deletion mutant of F. nucleatum 12230 by either electroporation or conjugation, DNA delivery via sonoporation, i.e., transient membrane permeabilization by ultrasound, was tested.

    Techniques:

    RT-PCR and Northern blot analyses of F. nucleatum 12230 and F. nucleatum 12230-US1. A. Schematic diagram showing locations of primers used for RT-PCR. The 2.4-kb fadA -containing fragment from F. nucleatum 12230 is presented as solid lines. The hairpin

    Journal: Journal of Bacteriology

    Article Title: Identification and Characterization of a Novel Adhesin Unique to Oral Fusobacteria

    doi: 10.1128/JB.187.15.5330-5340.2005

    Figure Lengend Snippet: RT-PCR and Northern blot analyses of F. nucleatum 12230 and F. nucleatum 12230-US1. A. Schematic diagram showing locations of primers used for RT-PCR. The 2.4-kb fadA -containing fragment from F. nucleatum 12230 is presented as solid lines. The hairpin

    Article Snippet: Following unsuccessful attempts to generate a correct fadA deletion mutant of F. nucleatum 12230 by either electroporation or conjugation, DNA delivery via sonoporation, i.e., transient membrane permeabilization by ultrasound, was tested.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Northern Blot

    I and II. Identification of F. nucleatum adhesins by far-Western analysis. a. F. nucleatum 12230 (I) or 40P (II) components stained with Coomassie blue following 12% SDS-PAGE. b. F. nucleatum 12230 (I) or 40P (II) components immobilized on PVDF membranes

    Journal: Journal of Bacteriology

    Article Title: Identification and Characterization of a Novel Adhesin Unique to Oral Fusobacteria

    doi: 10.1128/JB.187.15.5330-5340.2005

    Figure Lengend Snippet: I and II. Identification of F. nucleatum adhesins by far-Western analysis. a. F. nucleatum 12230 (I) or 40P (II) components stained with Coomassie blue following 12% SDS-PAGE. b. F. nucleatum 12230 (I) or 40P (II) components immobilized on PVDF membranes

    Article Snippet: Following unsuccessful attempts to generate a correct fadA deletion mutant of F. nucleatum 12230 by either electroporation or conjugation, DNA delivery via sonoporation, i.e., transient membrane permeabilization by ultrasound, was tested.

    Techniques: Western Blot, Staining, SDS Page

    Construction and screening of F. nucleatum 12230 cosmid library.

    Journal: Journal of Bacteriology

    Article Title: Identification and Characterization of a Novel Adhesin Unique to Oral Fusobacteria

    doi: 10.1128/JB.187.15.5330-5340.2005

    Figure Lengend Snippet: Construction and screening of F. nucleatum 12230 cosmid library.

    Article Snippet: Following unsuccessful attempts to generate a correct fadA deletion mutant of F. nucleatum 12230 by either electroporation or conjugation, DNA delivery via sonoporation, i.e., transient membrane permeabilization by ultrasound, was tested.

    Techniques:

    Inactivation of the  fadA  gene of  F. nucleatum  12230. A. Schematic diagram of construction of the Δ fadA :: erm  mutant by double-crossover allelic exchange. The erythromycin resistance cassette  ermF-ermAM  was inserted between bp 71 and 365 of the

    Journal: Journal of Bacteriology

    Article Title: Identification and Characterization of a Novel Adhesin Unique to Oral Fusobacteria

    doi: 10.1128/JB.187.15.5330-5340.2005

    Figure Lengend Snippet: Inactivation of the fadA gene of F. nucleatum 12230. A. Schematic diagram of construction of the Δ fadA :: erm mutant by double-crossover allelic exchange. The erythromycin resistance cassette ermF-ermAM was inserted between bp 71 and 365 of the

    Article Snippet: Following unsuccessful attempts to generate a correct fadA deletion mutant of F. nucleatum 12230 by either electroporation or conjugation, DNA delivery via sonoporation, i.e., transient membrane permeabilization by ultrasound, was tested.

    Techniques: Mutagenesis

    Attachment of F. nucleatum 12230 and F. nucleatum 12230-US1 to KB (hatched bars) and CHO (open bars) cells. The levels of attachment are means and standard deviations from three separate experiments, each performed in triplicate.

    Journal: Journal of Bacteriology

    Article Title: Identification and Characterization of a Novel Adhesin Unique to Oral Fusobacteria

    doi: 10.1128/JB.187.15.5330-5340.2005

    Figure Lengend Snippet: Attachment of F. nucleatum 12230 and F. nucleatum 12230-US1 to KB (hatched bars) and CHO (open bars) cells. The levels of attachment are means and standard deviations from three separate experiments, each performed in triplicate.

    Article Snippet: Following unsuccessful attempts to generate a correct fadA deletion mutant of F. nucleatum 12230 by either electroporation or conjugation, DNA delivery via sonoporation, i.e., transient membrane permeabilization by ultrasound, was tested.

    Techniques:

    2.1. F. nucleatum NanK production

    Journal: Acta Crystallographica. Section F, Structural Biology Communications

    Article Title: Crystal structure of N-acetylmannosamine kinase from Fusobacterium nucleatum

    doi: 10.1107/S2053230X17007439

    Figure Lengend Snippet: 2.1. F. nucleatum NanK production

    Article Snippet: The gene encoding F. nucleatum NanK was synthetically generated (GeneArt) and cloned into a pET300 NT/DEST expression vector containing an N-terminal His tag.

    Techniques:

    ( a ) Sialic acid catabolism in F. nucleatum . SiaT, transporter; NanA, lyase; NanK, kinase; NanE, epimerase; NagA, deacetylase; NagB, deaminase. ( b ) The chemical reaction catalyzed by N -acetylmannosamine kinase.

    Journal: Acta Crystallographica. Section F, Structural Biology Communications

    Article Title: Crystal structure of N-acetylmannosamine kinase from Fusobacterium nucleatum

    doi: 10.1107/S2053230X17007439

    Figure Lengend Snippet: ( a ) Sialic acid catabolism in F. nucleatum . SiaT, transporter; NanA, lyase; NanK, kinase; NanE, epimerase; NagA, deacetylase; NagB, deaminase. ( b ) The chemical reaction catalyzed by N -acetylmannosamine kinase.

    Article Snippet: The gene encoding F. nucleatum NanK was synthetically generated (GeneArt) and cloned into a pET300 NT/DEST expression vector containing an N-terminal His tag.

    Techniques: Histone Deacetylase Assay