f nucleatum Atcc Search Results


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  • 99
    Qiagen dna extraction kit
    Dna Extraction Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 5531 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore menadione
    Menadione, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1344 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    76
    ATCC present antimicrobial activity against f nucleatum atcc
    Present Antimicrobial Activity Against F Nucleatum Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 76/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC f nucleatum
    Evaluation of antimicrobial activity of the MNA-TNTs, GL13K-TNTs, and TNTs against ( A ) Porphyromonas gingivalis ATCC 33277 and ( B ) Fusobacterium <t>nucleatum</t> ATCC 25586, assessed using disk-diffusion assay. Abbreviations: MNA, metronidazole; MNA-TNTs, MNA-immobilized TNTs; TNTs, TiO 2 nanotubes; ATCC, American Type Culture Collection.
    F Nucleatum, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1278 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    ATCC f nucleatum 263
    Evaluation of antimicrobial activity of the MNA-TNTs, GL13K-TNTs, and TNTs against ( A ) Porphyromonas gingivalis ATCC 33277 and ( B ) Fusobacterium <t>nucleatum</t> ATCC 25586, assessed using disk-diffusion assay. Abbreviations: MNA, metronidazole; MNA-TNTs, MNA-immobilized TNTs; TNTs, TiO 2 nanotubes; ATCC, American Type Culture Collection.
    F Nucleatum 263, supplied by ATCC, used in various techniques. Bioz Stars score: 79/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC whole f nucleatum
    Evaluation of antimicrobial activity of the MNA-TNTs, GL13K-TNTs, and TNTs against ( A ) Porphyromonas gingivalis ATCC 33277 and ( B ) Fusobacterium <t>nucleatum</t> ATCC 25586, assessed using disk-diffusion assay. Abbreviations: MNA, metronidazole; MNA-TNTs, MNA-immobilized TNTs; TNTs, TiO 2 nanotubes; ATCC, American Type Culture Collection.
    Whole F Nucleatum, supplied by ATCC, used in various techniques. Bioz Stars score: 80/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    ATCC f nucleatum cells
    radD and cmpA expression: WT Fusobacterium <t>nucleatum</t> was grown in Columbia broth for 25 hr. Cell samples were collected every 3 hr and (a) OD 600 was measured, as well as (b) radD and cmpA expression by qRT ‐ PCR . Gene expression was normalized to rpoB and compare to the first time point. The dashed line was added to aid in the comparison. (c) radD and cmpA expression were also measured in cells from Fn biofilm grown overnight in SHI ‐ FSMS . Gene expression was normalized to rpoB and compare to planktonic cells grown under the same conditions. Each value represents means and standard deviation of at least three independent experiments. To aid with visualization, the dashed line represents no change
    F Nucleatum Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    ATCC infection f nucleatum
    radD and cmpA expression: WT Fusobacterium <t>nucleatum</t> was grown in Columbia broth for 25 hr. Cell samples were collected every 3 hr and (a) OD 600 was measured, as well as (b) radD and cmpA expression by qRT ‐ PCR . Gene expression was normalized to rpoB and compare to the first time point. The dashed line was added to aid in the comparison. (c) radD and cmpA expression were also measured in cells from Fn biofilm grown overnight in SHI ‐ FSMS . Gene expression was normalized to rpoB and compare to planktonic cells grown under the same conditions. Each value represents means and standard deviation of at least three independent experiments. To aid with visualization, the dashed line represents no change
    Infection F Nucleatum, supplied by ATCC, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    ATCC f nucleatum cell wall
    RT-PCR analysis of hBD2 mRNA induction in HOECs at different steps of purification. A , RT-PCR analysis was performed with RNA from HOEC monolayers treated with phorbol 12-myristate 13-acetate ( PMA ) as positive control ( lane 2 ) or 10 μg of F. <t>nucleatum</t>
    F Nucleatum Cell Wall, supplied by ATCC, used in various techniques. Bioz Stars score: 79/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    ATCC f nucleatum types strain
    RT-PCR analysis of hBD2 mRNA induction in HOECs at different steps of purification. A , RT-PCR analysis was performed with RNA from HOEC monolayers treated with phorbol 12-myristate 13-acetate ( PMA ) as positive control ( lane 2 ) or 10 μg of F. <t>nucleatum</t>
    F Nucleatum Types Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    ATCC bactericidal activity against f nucleatum
    RT-PCR analysis of hBD2 mRNA induction in HOECs at different steps of purification. A , RT-PCR analysis was performed with RNA from HOEC monolayers treated with phorbol 12-myristate 13-acetate ( PMA ) as positive control ( lane 2 ) or 10 μg of F. <t>nucleatum</t>
    Bactericidal Activity Against F Nucleatum, supplied by ATCC, used in various techniques. Bioz Stars score: 83/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    ATCC additional sequenced f nucleatum strains
    RT-PCR analysis of hBD2 mRNA induction in HOECs at different steps of purification. A , RT-PCR analysis was performed with RNA from HOEC monolayers treated with phorbol 12-myristate 13-acetate ( PMA ) as positive control ( lane 2 ) or 10 μg of F. <t>nucleatum</t>
    Additional Sequenced F Nucleatum Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    ATCC f nucleatum genomic dna samples
    RT-PCR analysis of hBD2 mRNA induction in HOECs at different steps of purification. A , RT-PCR analysis was performed with RNA from HOEC monolayers treated with phorbol 12-myristate 13-acetate ( PMA ) as positive control ( lane 2 ) or 10 μg of F. <t>nucleatum</t>
    F Nucleatum Genomic Dna Samples, supplied by ATCC, used in various techniques. Bioz Stars score: 88/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    ATCC p intermedia atcc
    RT-PCR analysis of hBD2 mRNA induction in HOECs at different steps of purification. A , RT-PCR analysis was performed with RNA from HOEC monolayers treated with phorbol 12-myristate 13-acetate ( PMA ) as positive control ( lane 2 ) or 10 μg of F. <t>nucleatum</t>
    P Intermedia Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 82/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    ATCC s sanguinis
    RT-PCR analysis of hBD2 mRNA induction in HOECs at different steps of purification. A , RT-PCR analysis was performed with RNA from HOEC monolayers treated with phorbol 12-myristate 13-acetate ( PMA ) as positive control ( lane 2 ) or 10 μg of F. <t>nucleatum</t>
    S Sanguinis, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    ATCC p acnes
    RT-PCR analysis of hBD2 mRNA induction in HOECs at different steps of purification. A , RT-PCR analysis was performed with RNA from HOEC monolayers treated with phorbol 12-myristate 13-acetate ( PMA ) as positive control ( lane 2 ) or 10 μg of F. <t>nucleatum</t>
    P Acnes, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    ATCC p intermedia
    RT-PCR analysis of hBD2 mRNA induction in HOECs at different steps of purification. A , RT-PCR analysis was performed with RNA from HOEC monolayers treated with phorbol 12-myristate 13-acetate ( PMA ) as positive control ( lane 2 ) or 10 μg of F. <t>nucleatum</t>
    P Intermedia, supplied by ATCC, used in various techniques. Bioz Stars score: 81/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    ATCC s mitis
    RT-PCR analysis of hBD2 mRNA induction in HOECs at different steps of purification. A , RT-PCR analysis was performed with RNA from HOEC monolayers treated with phorbol 12-myristate 13-acetate ( PMA ) as positive control ( lane 2 ) or 10 μg of F. <t>nucleatum</t>
    S Mitis, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Sartorius AG cellulose acetate membranes
    RT-PCR analysis of hBD2 mRNA induction in HOECs at different steps of purification. A , RT-PCR analysis was performed with RNA from HOEC monolayers treated with phorbol 12-myristate 13-acetate ( PMA ) as positive control ( lane 2 ) or 10 μg of F. <t>nucleatum</t>
    Cellulose Acetate Membranes, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 90/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    76
    ATCC bacterial culture l acidophilus
    RT-PCR analysis of hBD2 mRNA induction in HOECs at different steps of purification. A , RT-PCR analysis was performed with RNA from HOEC monolayers treated with phorbol 12-myristate 13-acetate ( PMA ) as positive control ( lane 2 ) or 10 μg of F. <t>nucleatum</t>
    Bacterial Culture L Acidophilus, supplied by ATCC, used in various techniques. Bioz Stars score: 76/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC porphyromonas gingivalis
    RT-PCR analysis of hBD2 mRNA induction in HOECs at different steps of purification. A , RT-PCR analysis was performed with RNA from HOEC monolayers treated with phorbol 12-myristate 13-acetate ( PMA ) as positive control ( lane 2 ) or 10 μg of F. <t>nucleatum</t>
    Porphyromonas Gingivalis, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 304 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher brain heart infusion broth
    RT-PCR analysis of hBD2 mRNA induction in HOECs at different steps of purification. A , RT-PCR analysis was performed with RNA from HOEC monolayers treated with phorbol 12-myristate 13-acetate ( PMA ) as positive control ( lane 2 ) or 10 μg of F. <t>nucleatum</t>
    Brain Heart Infusion Broth, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1839 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC p gingivalis
    Stimulation of PGLYRP2 and hBD2 expression in oral epithelial cells by bacteria. (A and B) TIGK cells were cultured with wild-type P. <t>gingivalis</t> or P. gingivalis KDP136 (Kgp/Rgp-null mutant) at an MOI of 100:1 for the times indicated. PGLYRP2 (A) and hBD2 (B) mRNA levels were then measured ( n = 3). (C to H) TIGK cells were cultured with F. nucleatum (C and F), S. gordonii (D and G), and S. sanguinis (E and H) at an MOI of 100:1 for the times indicated. PGLYRP2 (C to E) and hBD2 (F to H) mRNA levels were then measured ( n = 3). ***, P
    P Gingivalis, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2015 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    ATCC s gordonii
    Stimulation of PGLYRP2 and hBD2 expression in oral epithelial cells by bacteria. (A and B) TIGK cells were cultured with wild-type P. gingivalis or P. gingivalis KDP136 (Kgp/Rgp-null mutant) at an MOI of 100:1 for the times indicated. PGLYRP2 (A) and hBD2 (B) mRNA levels were then measured ( n = 3). (C to H) TIGK cells were cultured with F. nucleatum (C and F), S. <t>gordonii</t> (D and G), and S. sanguinis (E and H) at an MOI of 100:1 for the times indicated. PGLYRP2 (C to E) and hBD2 (F to H) mRNA levels were then measured ( n = 3). ***, P
    S Gordonii, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC t denticola
    Stimulation of PGLYRP2 and hBD2 expression in oral epithelial cells by bacteria. (A and B) TIGK cells were cultured with wild-type P. gingivalis or P. gingivalis KDP136 (Kgp/Rgp-null mutant) at an MOI of 100:1 for the times indicated. PGLYRP2 (A) and hBD2 (B) mRNA levels were then measured ( n = 3). (C to H) TIGK cells were cultured with F. nucleatum (C and F), S. <t>gordonii</t> (D and G), and S. sanguinis (E and H) at an MOI of 100:1 for the times indicated. PGLYRP2 (C to E) and hBD2 (F to H) mRNA levels were then measured ( n = 3). ***, P
    T Denticola, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 326 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    ATCC peptostreptococcus anaerobius
    Stimulation of PGLYRP2 and hBD2 expression in oral epithelial cells by bacteria. (A and B) TIGK cells were cultured with wild-type P. gingivalis or P. gingivalis KDP136 (Kgp/Rgp-null mutant) at an MOI of 100:1 for the times indicated. PGLYRP2 (A) and hBD2 (B) mRNA levels were then measured ( n = 3). (C to H) TIGK cells were cultured with F. nucleatum (C and F), S. <t>gordonii</t> (D and G), and S. sanguinis (E and H) at an MOI of 100:1 for the times indicated. PGLYRP2 (C to E) and hBD2 (F to H) mRNA levels were then measured ( n = 3). ***, P
    Peptostreptococcus Anaerobius, supplied by ATCC, used in various techniques. Bioz Stars score: 88/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    ATCC peptostreptococcus micros
    Stimulation of PGLYRP2 and hBD2 expression in oral epithelial cells by bacteria. (A and B) TIGK cells were cultured with wild-type P. gingivalis or P. gingivalis KDP136 (Kgp/Rgp-null mutant) at an MOI of 100:1 for the times indicated. PGLYRP2 (A) and hBD2 (B) mRNA levels were then measured ( n = 3). (C to H) TIGK cells were cultured with F. nucleatum (C and F), S. <t>gordonii</t> (D and G), and S. sanguinis (E and H) at an MOI of 100:1 for the times indicated. PGLYRP2 (C to E) and hBD2 (F to H) mRNA levels were then measured ( n = 3). ***, P
    Peptostreptococcus Micros, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    ATCC peptostreptococcus magnus
    Stimulation of PGLYRP2 and hBD2 expression in oral epithelial cells by bacteria. (A and B) TIGK cells were cultured with wild-type P. gingivalis or P. gingivalis KDP136 (Kgp/Rgp-null mutant) at an MOI of 100:1 for the times indicated. PGLYRP2 (A) and hBD2 (B) mRNA levels were then measured ( n = 3). (C to H) TIGK cells were cultured with F. nucleatum (C and F), S. <t>gordonii</t> (D and G), and S. sanguinis (E and H) at an MOI of 100:1 for the times indicated. PGLYRP2 (C to E) and hBD2 (F to H) mRNA levels were then measured ( n = 3). ***, P
    Peptostreptococcus Magnus, supplied by ATCC, used in various techniques. Bioz Stars score: 78/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC prevotella intermedia
    Stimulation of PGLYRP2 and hBD2 expression in oral epithelial cells by bacteria. (A and B) TIGK cells were cultured with wild-type P. gingivalis or P. gingivalis KDP136 (Kgp/Rgp-null mutant) at an MOI of 100:1 for the times indicated. PGLYRP2 (A) and hBD2 (B) mRNA levels were then measured ( n = 3). (C to H) TIGK cells were cultured with F. nucleatum (C and F), S. <t>gordonii</t> (D and G), and S. sanguinis (E and H) at an MOI of 100:1 for the times indicated. PGLYRP2 (C to E) and hBD2 (F to H) mRNA levels were then measured ( n = 3). ***, P
    Prevotella Intermedia, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    ATCC actinomyces oris
    Seven-day cultures of Tannerella sp. HOT-286 (SP18_24) showing growth stimulation by several of the 8 potential helper strains tested: ( a ) Streptococcus <t>oralis</t> , ( b ) Veillonella dispar , ( c ) Parvimonas micra , ( d ) Actinomyces <t>oris</t> , ( e ) Porphyromonas gingivalis , ( f ) Prevotella intermedia , ( g ) Propionibacterium acnes , and ( h ) Fusobacterium nucleatum .
    Actinomyces Oris, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    ATCC aerobic bacteria p aeruginosa
    Seven-day cultures of Tannerella sp. HOT-286 (SP18_24) showing growth stimulation by several of the 8 potential helper strains tested: ( a ) Streptococcus <t>oralis</t> , ( b ) Veillonella dispar , ( c ) Parvimonas micra , ( d ) Actinomyces <t>oris</t> , ( e ) Porphyromonas gingivalis , ( f ) Prevotella intermedia , ( g ) Propionibacterium acnes , and ( h ) Fusobacterium nucleatum .
    Aerobic Bacteria P Aeruginosa, supplied by ATCC, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Evaluation of antimicrobial activity of the MNA-TNTs, GL13K-TNTs, and TNTs against ( A ) Porphyromonas gingivalis ATCC 33277 and ( B ) Fusobacterium nucleatum ATCC 25586, assessed using disk-diffusion assay. Abbreviations: MNA, metronidazole; MNA-TNTs, MNA-immobilized TNTs; TNTs, TiO 2 nanotubes; ATCC, American Type Culture Collection.

    Journal: International Journal of Nanomedicine

    Article Title: Antibacterial activity and cytocompatibility of an implant coating consisting of TiO2 nanotubes combined with a GL13K antimicrobial peptide

    doi: 10.2147/IJN.S128775

    Figure Lengend Snippet: Evaluation of antimicrobial activity of the MNA-TNTs, GL13K-TNTs, and TNTs against ( A ) Porphyromonas gingivalis ATCC 33277 and ( B ) Fusobacterium nucleatum ATCC 25586, assessed using disk-diffusion assay. Abbreviations: MNA, metronidazole; MNA-TNTs, MNA-immobilized TNTs; TNTs, TiO 2 nanotubes; ATCC, American Type Culture Collection.

    Article Snippet: Antimicrobial activity assessment The antimicrobial activities of the specimens against two Gram-negative anaerobic bacterial strains, F. nucleatum (American Type Culture Collection [ATCC] 25586) and P. gingivalis (ATCC 33277), were assessed using a disk-diffusion assay (Kirby–Bauer).

    Techniques: Activity Assay, Diffusion-based Assay

    TLR2/TLR4 signaling modulates F . nucleatum -induced inflammation in vivo.

    Journal: PLoS ONE

    Article Title: TLR2/TLR4 activation induces Tregs and suppresses intestinal inflammation caused by Fusobacterium nucleatum in vivo

    doi: 10.1371/journal.pone.0186179

    Figure Lengend Snippet: TLR2/TLR4 signaling modulates F . nucleatum -induced inflammation in vivo.

    Article Snippet: F . nucleatum (ATCC 25586) was obtained from the American Type Culture Collection, and the specific culture method has been previously described [ ].

    Techniques: In Vivo

    F . nucleatum infection increased the ratio of regulatory T cells in vivo.

    Journal: PLoS ONE

    Article Title: TLR2/TLR4 activation induces Tregs and suppresses intestinal inflammation caused by Fusobacterium nucleatum in vivo

    doi: 10.1371/journal.pone.0186179

    Figure Lengend Snippet: F . nucleatum infection increased the ratio of regulatory T cells in vivo.

    Article Snippet: F . nucleatum (ATCC 25586) was obtained from the American Type Culture Collection, and the specific culture method has been previously described [ ].

    Techniques: Infection, In Vivo

    TLR2/TLR4 are involved in the F . nucleatum -induced inflammatory signaling pathway.

    Journal: PLoS ONE

    Article Title: TLR2/TLR4 activation induces Tregs and suppresses intestinal inflammation caused by Fusobacterium nucleatum in vivo

    doi: 10.1371/journal.pone.0186179

    Figure Lengend Snippet: TLR2/TLR4 are involved in the F . nucleatum -induced inflammatory signaling pathway.

    Article Snippet: F . nucleatum (ATCC 25586) was obtained from the American Type Culture Collection, and the specific culture method has been previously described [ ].

    Techniques:

    TLR2/TLR4 regulate F . nucleatum -induced inflammatory cytokines through Tregs in vivo.

    Journal: PLoS ONE

    Article Title: TLR2/TLR4 activation induces Tregs and suppresses intestinal inflammation caused by Fusobacterium nucleatum in vivo

    doi: 10.1371/journal.pone.0186179

    Figure Lengend Snippet: TLR2/TLR4 regulate F . nucleatum -induced inflammatory cytokines through Tregs in vivo.

    Article Snippet: F . nucleatum (ATCC 25586) was obtained from the American Type Culture Collection, and the specific culture method has been previously described [ ].

    Techniques: In Vivo

    Cytokines production by WT and MyD88-deficient BMDMs in response to F. nucleatum and A. actinomycetemcomitans infection. BMDMs from WT and MyD88-deficient mice were infected with F. nucleatum or A. actinomycetemcomitans for 6 h, and, as indicated, the

    Journal: Infection and Immunity

    Article Title: Diverse Toll-Like Receptors Mediate Cytokine Production by Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans in Macrophages

    doi: 10.1128/IAI.01226-13

    Figure Lengend Snippet: Cytokines production by WT and MyD88-deficient BMDMs in response to F. nucleatum and A. actinomycetemcomitans infection. BMDMs from WT and MyD88-deficient mice were infected with F. nucleatum or A. actinomycetemcomitans for 6 h, and, as indicated, the

    Article Snippet: F. nucleatum (ATCC 25586) and A. actinomycetemcomitans (ATCC 43718) were purchased from the American Type Culture Collection (Manassas, VA, USA).

    Techniques: Infection, Mouse Assay

    Production of IL-6 and TNF-α in WT and TLR2/4- and MyD88-deficient BMDMs in response to F. nucleatum and A. actinomycetemcomitans infection. WT and TLR2/4-, and MyD88-deficient BMDMs were infected with F. nucleatum and A. actinomycetemcomitans

    Journal: Infection and Immunity

    Article Title: Diverse Toll-Like Receptors Mediate Cytokine Production by Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans in Macrophages

    doi: 10.1128/IAI.01226-13

    Figure Lengend Snippet: Production of IL-6 and TNF-α in WT and TLR2/4- and MyD88-deficient BMDMs in response to F. nucleatum and A. actinomycetemcomitans infection. WT and TLR2/4-, and MyD88-deficient BMDMs were infected with F. nucleatum and A. actinomycetemcomitans

    Article Snippet: F. nucleatum (ATCC 25586) and A. actinomycetemcomitans (ATCC 43718) were purchased from the American Type Culture Collection (Manassas, VA, USA).

    Techniques: Infection

    Effect of NF-κB and MAPKs on F. nucleatum - and A. actinomycetemcomitans -induced production of cytokines by macrophages. WT BMDMs were pretreated with various doses of each inhibitor 2 h before infection. The cells were then infected with F. nucleatum

    Journal: Infection and Immunity

    Article Title: Diverse Toll-Like Receptors Mediate Cytokine Production by Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans in Macrophages

    doi: 10.1128/IAI.01226-13

    Figure Lengend Snippet: Effect of NF-κB and MAPKs on F. nucleatum - and A. actinomycetemcomitans -induced production of cytokines by macrophages. WT BMDMs were pretreated with various doses of each inhibitor 2 h before infection. The cells were then infected with F. nucleatum

    Article Snippet: F. nucleatum (ATCC 25586) and A. actinomycetemcomitans (ATCC 43718) were purchased from the American Type Culture Collection (Manassas, VA, USA).

    Techniques: Infection

    Effect of endosomal TLRs and bacterial DNA on cytokine production by macrophages in response to F. nucleatum or A. actinomycetemcomitans infection. TLR2/4-deficient BMDMs were pretreated with chloroquine (CLQ) 2 h before infection. The cells were then

    Journal: Infection and Immunity

    Article Title: Diverse Toll-Like Receptors Mediate Cytokine Production by Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans in Macrophages

    doi: 10.1128/IAI.01226-13

    Figure Lengend Snippet: Effect of endosomal TLRs and bacterial DNA on cytokine production by macrophages in response to F. nucleatum or A. actinomycetemcomitans infection. TLR2/4-deficient BMDMs were pretreated with chloroquine (CLQ) 2 h before infection. The cells were then

    Article Snippet: F. nucleatum (ATCC 25586) and A. actinomycetemcomitans (ATCC 43718) were purchased from the American Type Culture Collection (Manassas, VA, USA).

    Techniques: Infection

    Production of IL-6 and TNF-α by WT and TLR2-, TLR4-, and TLR2/4-deficient BMDMs in response to F. nucleatum and A. actinomycetemcomitans . BMDMs were infected with F. nucleatum or A. actinomycetemcomitans at the indicated MOI. At 6 (A to D) or

    Journal: Infection and Immunity

    Article Title: Diverse Toll-Like Receptors Mediate Cytokine Production by Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans in Macrophages

    doi: 10.1128/IAI.01226-13

    Figure Lengend Snippet: Production of IL-6 and TNF-α by WT and TLR2-, TLR4-, and TLR2/4-deficient BMDMs in response to F. nucleatum and A. actinomycetemcomitans . BMDMs were infected with F. nucleatum or A. actinomycetemcomitans at the indicated MOI. At 6 (A to D) or

    Article Snippet: F. nucleatum (ATCC 25586) and A. actinomycetemcomitans (ATCC 43718) were purchased from the American Type Culture Collection (Manassas, VA, USA).

    Techniques: Infection

    NF-κB and MAPK activation in WT and TLR2/4-, and MyD88-deficient BMDMs in response to F. nucleatum and A. actinomycetemcomitans infection. Cells were infected with F. nucleatum or A. actinomycetemcomitans at an MOI of 1/100, and cellular protein

    Journal: Infection and Immunity

    Article Title: Diverse Toll-Like Receptors Mediate Cytokine Production by Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans in Macrophages

    doi: 10.1128/IAI.01226-13

    Figure Lengend Snippet: NF-κB and MAPK activation in WT and TLR2/4-, and MyD88-deficient BMDMs in response to F. nucleatum and A. actinomycetemcomitans infection. Cells were infected with F. nucleatum or A. actinomycetemcomitans at an MOI of 1/100, and cellular protein

    Article Snippet: F. nucleatum (ATCC 25586) and A. actinomycetemcomitans (ATCC 43718) were purchased from the American Type Culture Collection (Manassas, VA, USA).

    Techniques: Activation Assay, Infection

    Increased levels of F. nucleatum are detected in stool of CRC patients. ( a ) A Beeswarm Boxplot is used to illustrate the relative levels of F. nucleatum in stool of control patients, and patients diagnosed with dysplasia or cancer. Horisontal lines indicate median (in bold) and quartiles. ( b ) An ROC curve displaying the specificity and the sensitivity for the F. nucleatum assay. The ROC curve was calculated using the variable for F. nucleatum and cancer/no cancer. The level of F. nucleatum in each sample is given as a relative quantification with the total microbial 16S rRNA gene DNA in each sample as reference 2 ∧ (−ΔCq) , ΔCq = Cq F. nucleatum − Cq16 S rRNA gene ).

    Journal: International Journal of Cancer

    Article Title: Cancer‐associated fecal microbial markers in colorectal cancer detection

    doi: 10.1002/ijc.31011

    Figure Lengend Snippet: Increased levels of F. nucleatum are detected in stool of CRC patients. ( a ) A Beeswarm Boxplot is used to illustrate the relative levels of F. nucleatum in stool of control patients, and patients diagnosed with dysplasia or cancer. Horisontal lines indicate median (in bold) and quartiles. ( b ) An ROC curve displaying the specificity and the sensitivity for the F. nucleatum assay. The ROC curve was calculated using the variable for F. nucleatum and cancer/no cancer. The level of F. nucleatum in each sample is given as a relative quantification with the total microbial 16S rRNA gene DNA in each sample as reference 2 ∧ (−ΔCq) , ΔCq = Cq F. nucleatum − Cq16 S rRNA gene ).

    Article Snippet: F. nucleatum subsp. nucleatum Knorr (ATCC 25586 D‐5) was used as positive control.

    Techniques:

    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces naeslundii (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: In vitro Increased Respiratory Activity of Selected Oral Bacteria May Explain Competitive and Collaborative Interactions in the Oral Microbiome

    doi: 10.3389/fcimb.2017.00235

    Figure Lengend Snippet: Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces naeslundii (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.

    Article Snippet: Bacterial cultures and collection The following strains commonly present in the oral microbiome (Aas et al., ; Maddi and Scannapieco, ; Loozen et al., ) were obtained from the American Type Culture Collection (ATCC): A . actinomycetemcomitans (ATCC 43718), F. nucleatum (ATCC 10953), P. gingivalis (ATCC 33277), P. intermedia (ATCC 25611), S. mutans (ATCC 25175), S. sobrinus (ATCC 33478), T. forsythia (ATCC 43037), Actinomyces naeslundii (ATCC 51655), Capnocytophaga sputigena (ATCC 33612), Streptococcus gordonii (ATCC 49818), Actinomyces viscosus (ATCC 15987), and S. mitis (ATCC 49456).

    Techniques: Activity Assay

    Membrane potential of Porphyromonas gingivalis , Clostridium perfringens , and Fusobacterium nucleatum treated with HTS-4 and AGS. AGS, Panax quinquefolius leaf-stem; AU, absorbance units; HTS, heat-transformed saponins; MBC, minimum bactericidal concentration; MIC, minimum inhibitory concentration; NC, negative control.

    Journal: Journal of Ginseng Research

    Article Title: Improved antimicrobial effect of ginseng extract by heat transformation

    doi: 10.1016/j.jgr.2016.03.002

    Figure Lengend Snippet: Membrane potential of Porphyromonas gingivalis , Clostridium perfringens , and Fusobacterium nucleatum treated with HTS-4 and AGS. AGS, Panax quinquefolius leaf-stem; AU, absorbance units; HTS, heat-transformed saponins; MBC, minimum bactericidal concentration; MIC, minimum inhibitory concentration; NC, negative control.

    Article Snippet: F. nucleatum (ATCC 10953), C. perfringens (ATCC 13124), and P. gingivalis (ATCC 33277) were cultured in CDC anaerobic blood agar base medium for 48 h at 37°C in a YQX-II anaerobic incubator (Shanghai, China) for further use.

    Techniques: Transformation Assay, Concentration Assay, Negative Control

    Protein release. Release of protein from (A) Porphyromonas gingivalis , (B) Clostridium perfringens , and (C) Fusobacterium nucleatum treated with HTS-4 and AGS. AGS, Panax quinquefolius leaf-stem; HTS, heat-transformed saponins; MBC, minimum bactericidal concentration; MIC, minimum inhibitory concentration; NC, negative control.

    Journal: Journal of Ginseng Research

    Article Title: Improved antimicrobial effect of ginseng extract by heat transformation

    doi: 10.1016/j.jgr.2016.03.002

    Figure Lengend Snippet: Protein release. Release of protein from (A) Porphyromonas gingivalis , (B) Clostridium perfringens , and (C) Fusobacterium nucleatum treated with HTS-4 and AGS. AGS, Panax quinquefolius leaf-stem; HTS, heat-transformed saponins; MBC, minimum bactericidal concentration; MIC, minimum inhibitory concentration; NC, negative control.

    Article Snippet: F. nucleatum (ATCC 10953), C. perfringens (ATCC 13124), and P. gingivalis (ATCC 33277) were cultured in CDC anaerobic blood agar base medium for 48 h at 37°C in a YQX-II anaerobic incubator (Shanghai, China) for further use.

    Techniques: Transformation Assay, Concentration Assay, Negative Control

    Nucleic acid absorbance. Release of 260-nm absorbing material from (A) Porphyromonas gingivalis , (B) Clostridium perfringens , and (C) Fusobacterium nucleatum treated with HTS-4 and AGS. AGS, Panax quinquefolius leaf-stem; HTS, heat-transformed saponins; MBC, minimum bactericidal concentration; MIC, minimum inhibitory concentration; NC, negative control.

    Journal: Journal of Ginseng Research

    Article Title: Improved antimicrobial effect of ginseng extract by heat transformation

    doi: 10.1016/j.jgr.2016.03.002

    Figure Lengend Snippet: Nucleic acid absorbance. Release of 260-nm absorbing material from (A) Porphyromonas gingivalis , (B) Clostridium perfringens , and (C) Fusobacterium nucleatum treated with HTS-4 and AGS. AGS, Panax quinquefolius leaf-stem; HTS, heat-transformed saponins; MBC, minimum bactericidal concentration; MIC, minimum inhibitory concentration; NC, negative control.

    Article Snippet: F. nucleatum (ATCC 10953), C. perfringens (ATCC 13124), and P. gingivalis (ATCC 33277) were cultured in CDC anaerobic blood agar base medium for 48 h at 37°C in a YQX-II anaerobic incubator (Shanghai, China) for further use.

    Techniques: Transformation Assay, Concentration Assay, Negative Control

    radD and cmpA expression: WT Fusobacterium nucleatum was grown in Columbia broth for 25 hr. Cell samples were collected every 3 hr and (a) OD 600 was measured, as well as (b) radD and cmpA expression by qRT ‐ PCR . Gene expression was normalized to rpoB and compare to the first time point. The dashed line was added to aid in the comparison. (c) radD and cmpA expression were also measured in cells from Fn biofilm grown overnight in SHI ‐ FSMS . Gene expression was normalized to rpoB and compare to planktonic cells grown under the same conditions. Each value represents means and standard deviation of at least three independent experiments. To aid with visualization, the dashed line represents no change

    Journal: MicrobiologyOpen

    Article Title: Identification and characterization of a novel Fusobacterium nucleatum adhesin involved in physical interaction and biofilm formation with Streptococcus gordonii. Identification and characterization of a novel Fusobacterium nucleatum adhesin involved in physical interaction and biofilm formation with Streptococcus gordonii

    doi: 10.1002/mbo3.444

    Figure Lengend Snippet: radD and cmpA expression: WT Fusobacterium nucleatum was grown in Columbia broth for 25 hr. Cell samples were collected every 3 hr and (a) OD 600 was measured, as well as (b) radD and cmpA expression by qRT ‐ PCR . Gene expression was normalized to rpoB and compare to the first time point. The dashed line was added to aid in the comparison. (c) radD and cmpA expression were also measured in cells from Fn biofilm grown overnight in SHI ‐ FSMS . Gene expression was normalized to rpoB and compare to planktonic cells grown under the same conditions. Each value represents means and standard deviation of at least three independent experiments. To aid with visualization, the dashed line represents no change

    Article Snippet: 3.1 Multiple F. nucleatum adhesins are involved in its coaggregation with S. gordonii For detailed characterization of the physical interaction between F. nucleatum and S. gordonii , we collected F. nucleatum cells, ATCC strain 23726, that were in stationary phase, after overnight growth, and cells that were in late exponential phase to perform coaggregation experiments with midexponential phase S. gordonii V288 cells.

    Techniques: Expressing, Quantitative RT-PCR, Standard Deviation

    Quantitative coaggregation of (a) wild‐type ( WT ) Streptococcus gordonii strain V288 and the WT Fusobacterium nucleatum strain ATCC 23726, or the radD mutant derivative, at different phases of growth. (b) Quantitative coaggregation of WT S. gordonii strains ( DL 1, V288, ATCC 10558, and ATCC 51656) with WT F. nucleatum strain ATCC 23726, or the radD mutant derivative at exponential growth. Data represent means and standard deviation of percent coaggregation of at least three independent experiments. * p

    Journal: MicrobiologyOpen

    Article Title: Identification and characterization of a novel Fusobacterium nucleatum adhesin involved in physical interaction and biofilm formation with Streptococcus gordonii. Identification and characterization of a novel Fusobacterium nucleatum adhesin involved in physical interaction and biofilm formation with Streptococcus gordonii

    doi: 10.1002/mbo3.444

    Figure Lengend Snippet: Quantitative coaggregation of (a) wild‐type ( WT ) Streptococcus gordonii strain V288 and the WT Fusobacterium nucleatum strain ATCC 23726, or the radD mutant derivative, at different phases of growth. (b) Quantitative coaggregation of WT S. gordonii strains ( DL 1, V288, ATCC 10558, and ATCC 51656) with WT F. nucleatum strain ATCC 23726, or the radD mutant derivative at exponential growth. Data represent means and standard deviation of percent coaggregation of at least three independent experiments. * p

    Article Snippet: 3.1 Multiple F. nucleatum adhesins are involved in its coaggregation with S. gordonii For detailed characterization of the physical interaction between F. nucleatum and S. gordonii , we collected F. nucleatum cells, ATCC strain 23726, that were in stationary phase, after overnight growth, and cells that were in late exponential phase to perform coaggregation experiments with midexponential phase S. gordonii V288 cells.

    Techniques: Mutagenesis, Standard Deviation

    Quantitative coaggregation of wild‐type Streptococcus gordonii strain (a) V288, (b) ATCC 10558, (c) DL 1, and (d) ATCC 51656 with Fusobacterium nucleatum strains: Wild‐type ( WT ) and the mutant derivatives: radD , cmpA , and radD cmpA double mutant. Data represent means and standard deviation of percent coaggregation of at least three independent experiments. * p

    Journal: MicrobiologyOpen

    Article Title: Identification and characterization of a novel Fusobacterium nucleatum adhesin involved in physical interaction and biofilm formation with Streptococcus gordonii. Identification and characterization of a novel Fusobacterium nucleatum adhesin involved in physical interaction and biofilm formation with Streptococcus gordonii

    doi: 10.1002/mbo3.444

    Figure Lengend Snippet: Quantitative coaggregation of wild‐type Streptococcus gordonii strain (a) V288, (b) ATCC 10558, (c) DL 1, and (d) ATCC 51656 with Fusobacterium nucleatum strains: Wild‐type ( WT ) and the mutant derivatives: radD , cmpA , and radD cmpA double mutant. Data represent means and standard deviation of percent coaggregation of at least three independent experiments. * p

    Article Snippet: 3.1 Multiple F. nucleatum adhesins are involved in its coaggregation with S. gordonii For detailed characterization of the physical interaction between F. nucleatum and S. gordonii , we collected F. nucleatum cells, ATCC strain 23726, that were in stationary phase, after overnight growth, and cells that were in late exponential phase to perform coaggregation experiments with midexponential phase S. gordonii V288 cells.

    Techniques: Mutagenesis, Standard Deviation

    (a) Fusobacterium nucleatum radD cmpA double mutant (strain BL 83) was constructed by insertion of the inactivation plasmid pBPL 9 into cmpA (Fn1554) in a radD::catP mutant background. Black arrows indicate the location of primers used for mutant construction and analysis. (b) Confirmation of plasmid insertion into cmpA by PCR analysis of the cmpA mutant (m) with wild‐type (WT) as the control

    Journal: MicrobiologyOpen

    Article Title: Identification and characterization of a novel Fusobacterium nucleatum adhesin involved in physical interaction and biofilm formation with Streptococcus gordonii. Identification and characterization of a novel Fusobacterium nucleatum adhesin involved in physical interaction and biofilm formation with Streptococcus gordonii

    doi: 10.1002/mbo3.444

    Figure Lengend Snippet: (a) Fusobacterium nucleatum radD cmpA double mutant (strain BL 83) was constructed by insertion of the inactivation plasmid pBPL 9 into cmpA (Fn1554) in a radD::catP mutant background. Black arrows indicate the location of primers used for mutant construction and analysis. (b) Confirmation of plasmid insertion into cmpA by PCR analysis of the cmpA mutant (m) with wild‐type (WT) as the control

    Article Snippet: 3.1 Multiple F. nucleatum adhesins are involved in its coaggregation with S. gordonii For detailed characterization of the physical interaction between F. nucleatum and S. gordonii , we collected F. nucleatum cells, ATCC strain 23726, that were in stationary phase, after overnight growth, and cells that were in late exponential phase to perform coaggregation experiments with midexponential phase S. gordonii V288 cells.

    Techniques: Mutagenesis, Construct, Plasmid Preparation, Polymerase Chain Reaction

    Streptococcus gordonii and Fusobacterium nucleatum dual‐species biofilm: (a) Confocal laser scanning microscopy of syto9‐stained dual‐species biofilm after overnight incubation. S. gordonii (Sg) cells constitutively express mCherry from their chromosome and appear orange/yellow on the images. Wild‐type ( WT ) F. nucleatum (Fn) and its mutant derivatives ( radD , cmpA , and radD cmpA double mutant) are strained by syto9‐only and appear green on the images. (b) The presence of the Fn mutant strains in the Sg‐Fn dual‐species biofilm is displayed as the percentage of Fn cells normalized to the number of attached Sg cells/well, compared to that measured with WT Fn. Cellular ratios were determined by measuring DNA concentration by qPCR , targeting the F. nucleatum gene fomA and the S. gordonii gene srtA . At least three independent experiments were performed per strain combination. Each value represents means and standard deviation of at least three independent experiments. * p

    Journal: MicrobiologyOpen

    Article Title: Identification and characterization of a novel Fusobacterium nucleatum adhesin involved in physical interaction and biofilm formation with Streptococcus gordonii. Identification and characterization of a novel Fusobacterium nucleatum adhesin involved in physical interaction and biofilm formation with Streptococcus gordonii

    doi: 10.1002/mbo3.444

    Figure Lengend Snippet: Streptococcus gordonii and Fusobacterium nucleatum dual‐species biofilm: (a) Confocal laser scanning microscopy of syto9‐stained dual‐species biofilm after overnight incubation. S. gordonii (Sg) cells constitutively express mCherry from their chromosome and appear orange/yellow on the images. Wild‐type ( WT ) F. nucleatum (Fn) and its mutant derivatives ( radD , cmpA , and radD cmpA double mutant) are strained by syto9‐only and appear green on the images. (b) The presence of the Fn mutant strains in the Sg‐Fn dual‐species biofilm is displayed as the percentage of Fn cells normalized to the number of attached Sg cells/well, compared to that measured with WT Fn. Cellular ratios were determined by measuring DNA concentration by qPCR , targeting the F. nucleatum gene fomA and the S. gordonii gene srtA . At least three independent experiments were performed per strain combination. Each value represents means and standard deviation of at least three independent experiments. * p

    Article Snippet: 3.1 Multiple F. nucleatum adhesins are involved in its coaggregation with S. gordonii For detailed characterization of the physical interaction between F. nucleatum and S. gordonii , we collected F. nucleatum cells, ATCC strain 23726, that were in stationary phase, after overnight growth, and cells that were in late exponential phase to perform coaggregation experiments with midexponential phase S. gordonii V288 cells.

    Techniques: Confocal Laser Scanning Microscopy, Staining, Incubation, Mutagenesis, Concentration Assay, Real-time Polymerase Chain Reaction, Standard Deviation

    Quantitative coaggregation of wild‐type ( WT ) Streptococcus gordonii strain V288 with Fusobacterium nucleatum ATCC 23726 WT strain and OMP mutant derivatives ( radD , Fn254, cmpA , Fn1893, Fn2047, and aim1 ). Data represent means and standard deviation of percent coaggregation of at least three independent experiments. * p

    Journal: MicrobiologyOpen

    Article Title: Identification and characterization of a novel Fusobacterium nucleatum adhesin involved in physical interaction and biofilm formation with Streptococcus gordonii. Identification and characterization of a novel Fusobacterium nucleatum adhesin involved in physical interaction and biofilm formation with Streptococcus gordonii

    doi: 10.1002/mbo3.444

    Figure Lengend Snippet: Quantitative coaggregation of wild‐type ( WT ) Streptococcus gordonii strain V288 with Fusobacterium nucleatum ATCC 23726 WT strain and OMP mutant derivatives ( radD , Fn254, cmpA , Fn1893, Fn2047, and aim1 ). Data represent means and standard deviation of percent coaggregation of at least three independent experiments. * p

    Article Snippet: 3.1 Multiple F. nucleatum adhesins are involved in its coaggregation with S. gordonii For detailed characterization of the physical interaction between F. nucleatum and S. gordonii , we collected F. nucleatum cells, ATCC strain 23726, that were in stationary phase, after overnight growth, and cells that were in late exponential phase to perform coaggregation experiments with midexponential phase S. gordonii V288 cells.

    Techniques: Mutagenesis, Standard Deviation

    RT-PCR analysis of hBD2 mRNA induction in HOECs at different steps of purification. A , RT-PCR analysis was performed with RNA from HOEC monolayers treated with phorbol 12-myristate 13-acetate ( PMA ) as positive control ( lane 2 ) or 10 μg of F. nucleatum

    Journal: The Journal of Biological Chemistry

    Article Title: Fusobacterium nucleatum-associated ?-Defensin Inducer (FAD-I)

    doi: 10.1074/jbc.M110.133140

    Figure Lengend Snippet: RT-PCR analysis of hBD2 mRNA induction in HOECs at different steps of purification. A , RT-PCR analysis was performed with RNA from HOEC monolayers treated with phorbol 12-myristate 13-acetate ( PMA ) as positive control ( lane 2 ) or 10 μg of F. nucleatum

    Article Snippet: A systematic biochemical fractionation of the F. nucleatum cell wall (ATCC 25586), followed by isoelectric focusing, led to an active fraction containing four candidate proteins, whose genes were identified using the F. nucleatum genome ( ).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Purification, Positive Control

    Induction of hBD2 mRNA and protein by Pg1527 . A , Western blot analysis of cell wall preparations from P. gingivalis transformed with FN1527 ( Pg + 1527 ), P. gingivalis transformed with vector only ( Pg + vector ), F. nucleatum 25586 cell wall ( Fn ), or P.

    Journal: The Journal of Biological Chemistry

    Article Title: Fusobacterium nucleatum-associated ?-Defensin Inducer (FAD-I)

    doi: 10.1074/jbc.M110.133140

    Figure Lengend Snippet: Induction of hBD2 mRNA and protein by Pg1527 . A , Western blot analysis of cell wall preparations from P. gingivalis transformed with FN1527 ( Pg + 1527 ), P. gingivalis transformed with vector only ( Pg + vector ), F. nucleatum 25586 cell wall ( Fn ), or P.

    Article Snippet: A systematic biochemical fractionation of the F. nucleatum cell wall (ATCC 25586), followed by isoelectric focusing, led to an active fraction containing four candidate proteins, whose genes were identified using the F. nucleatum genome ( ).

    Techniques: Western Blot, Transformation Assay, Plasmid Preparation

    HOEC expression of hBD2 following challenge by cell extracts from E. coli expressing different F. nucleatum recombinant proteins and induction of hBD2 by affinity-purified FN1527 c-Myc. A , HOECs were treated with extracts (100 μg/ml total protein)

    Journal: The Journal of Biological Chemistry

    Article Title: Fusobacterium nucleatum-associated ?-Defensin Inducer (FAD-I)

    doi: 10.1074/jbc.M110.133140

    Figure Lengend Snippet: HOEC expression of hBD2 following challenge by cell extracts from E. coli expressing different F. nucleatum recombinant proteins and induction of hBD2 by affinity-purified FN1527 c-Myc. A , HOECs were treated with extracts (100 μg/ml total protein)

    Article Snippet: A systematic biochemical fractionation of the F. nucleatum cell wall (ATCC 25586), followed by isoelectric focusing, led to an active fraction containing four candidate proteins, whose genes were identified using the F. nucleatum genome ( ).

    Techniques: Expressing, Recombinant, Affinity Purification

    F. nucleatum cell wall and FAD-I induce hBD2 via TLR2. HOECs were treated with 5 μg/ml anti-TLR2 antibody or isotype control for 30 min and then stimulated with either 10 μg/ml F. nucleatum cell wall ( Fn-CW ) ( A ) or 10 μg/ml 1527

    Journal: The Journal of Biological Chemistry

    Article Title: Fusobacterium nucleatum-associated ?-Defensin Inducer (FAD-I)

    doi: 10.1074/jbc.M110.133140

    Figure Lengend Snippet: F. nucleatum cell wall and FAD-I induce hBD2 via TLR2. HOECs were treated with 5 μg/ml anti-TLR2 antibody or isotype control for 30 min and then stimulated with either 10 μg/ml F. nucleatum cell wall ( Fn-CW ) ( A ) or 10 μg/ml 1527

    Article Snippet: A systematic biochemical fractionation of the F. nucleatum cell wall (ATCC 25586), followed by isoelectric focusing, led to an active fraction containing four candidate proteins, whose genes were identified using the F. nucleatum genome ( ).

    Techniques:

    Stimulation of PGLYRP2 and hBD2 expression in oral epithelial cells by bacteria. (A and B) TIGK cells were cultured with wild-type P. gingivalis or P. gingivalis KDP136 (Kgp/Rgp-null mutant) at an MOI of 100:1 for the times indicated. PGLYRP2 (A) and hBD2 (B) mRNA levels were then measured ( n = 3). (C to H) TIGK cells were cultured with F. nucleatum (C and F), S. gordonii (D and G), and S. sanguinis (E and H) at an MOI of 100:1 for the times indicated. PGLYRP2 (C to E) and hBD2 (F to H) mRNA levels were then measured ( n = 3). ***, P

    Journal: Infection and Immunity

    Article Title: Regulation of the Peptidoglycan Amidase PGLYRP2 in Epithelial Cells by Interleukin-36γ

    doi: 10.1128/IAI.00384-18

    Figure Lengend Snippet: Stimulation of PGLYRP2 and hBD2 expression in oral epithelial cells by bacteria. (A and B) TIGK cells were cultured with wild-type P. gingivalis or P. gingivalis KDP136 (Kgp/Rgp-null mutant) at an MOI of 100:1 for the times indicated. PGLYRP2 (A) and hBD2 (B) mRNA levels were then measured ( n = 3). (C to H) TIGK cells were cultured with F. nucleatum (C and F), S. gordonii (D and G), and S. sanguinis (E and H) at an MOI of 100:1 for the times indicated. PGLYRP2 (C to E) and hBD2 (F to H) mRNA levels were then measured ( n = 3). ***, P

    Article Snippet: P. gingivalis (ATCC 33277), F. nucleatum (ATCC 10953), S. gordonii (ATCC 35105), and S. sanguinis (ATCC 10556) were obtained from the culture collection of the Melbourne Dental School (University of Melbourne).

    Techniques: Expressing, Cell Culture, Mutagenesis

    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces naeslundii (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: In vitro Increased Respiratory Activity of Selected Oral Bacteria May Explain Competitive and Collaborative Interactions in the Oral Microbiome

    doi: 10.3389/fcimb.2017.00235

    Figure Lengend Snippet: Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces naeslundii (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.

    Article Snippet: Bacterial cultures and collection The following strains commonly present in the oral microbiome (Aas et al., ; Maddi and Scannapieco, ; Loozen et al., ) were obtained from the American Type Culture Collection (ATCC): A . actinomycetemcomitans (ATCC 43718), F. nucleatum (ATCC 10953), P. gingivalis (ATCC 33277), P. intermedia (ATCC 25611), S. mutans (ATCC 25175), S. sobrinus (ATCC 33478), T. forsythia (ATCC 43037), Actinomyces naeslundii (ATCC 51655), Capnocytophaga sputigena (ATCC 33612), Streptococcus gordonii (ATCC 49818), Actinomyces viscosus (ATCC 15987), and S. mitis (ATCC 49456).

    Techniques: Activity Assay

    Stimulation of PGLYRP2 and hBD2 expression in oral epithelial cells by bacteria. (A and B) TIGK cells were cultured with wild-type P. gingivalis or P. gingivalis KDP136 (Kgp/Rgp-null mutant) at an MOI of 100:1 for the times indicated. PGLYRP2 (A) and hBD2 (B) mRNA levels were then measured ( n = 3). (C to H) TIGK cells were cultured with F. nucleatum (C and F), S. gordonii (D and G), and S. sanguinis (E and H) at an MOI of 100:1 for the times indicated. PGLYRP2 (C to E) and hBD2 (F to H) mRNA levels were then measured ( n = 3). ***, P

    Journal: Infection and Immunity

    Article Title: Regulation of the Peptidoglycan Amidase PGLYRP2 in Epithelial Cells by Interleukin-36γ

    doi: 10.1128/IAI.00384-18

    Figure Lengend Snippet: Stimulation of PGLYRP2 and hBD2 expression in oral epithelial cells by bacteria. (A and B) TIGK cells were cultured with wild-type P. gingivalis or P. gingivalis KDP136 (Kgp/Rgp-null mutant) at an MOI of 100:1 for the times indicated. PGLYRP2 (A) and hBD2 (B) mRNA levels were then measured ( n = 3). (C to H) TIGK cells were cultured with F. nucleatum (C and F), S. gordonii (D and G), and S. sanguinis (E and H) at an MOI of 100:1 for the times indicated. PGLYRP2 (C to E) and hBD2 (F to H) mRNA levels were then measured ( n = 3). ***, P

    Article Snippet: P. gingivalis (ATCC 33277), F. nucleatum (ATCC 10953), S. gordonii (ATCC 35105), and S. sanguinis (ATCC 10556) were obtained from the culture collection of the Melbourne Dental School (University of Melbourne).

    Techniques: Expressing, Cell Culture, Mutagenesis

    Seven-day cultures of Tannerella sp. HOT-286 (SP18_24) showing growth stimulation by several of the 8 potential helper strains tested: ( a ) Streptococcus oralis , ( b ) Veillonella dispar , ( c ) Parvimonas micra , ( d ) Actinomyces oris , ( e ) Porphyromonas gingivalis , ( f ) Prevotella intermedia , ( g ) Propionibacterium acnes , and ( h ) Fusobacterium nucleatum .

    Journal: Journal of Dental Research

    Article Title: First Cultivation of Health-Associated Tannerella sp. HOT-286 (BU063)

    doi: 10.1177/0022034516651078

    Figure Lengend Snippet: Seven-day cultures of Tannerella sp. HOT-286 (SP18_24) showing growth stimulation by several of the 8 potential helper strains tested: ( a ) Streptococcus oralis , ( b ) Veillonella dispar , ( c ) Parvimonas micra , ( d ) Actinomyces oris , ( e ) Porphyromonas gingivalis , ( f ) Prevotella intermedia , ( g ) Propionibacterium acnes , and ( h ) Fusobacterium nucleatum .

    Article Snippet: Small circular inocula of the following bacterial strains were applied to plates: Streptococcus oralis (NCTC 7864), Veillonella dispar (NCTC 11831), Actinomyces oris (ATCC 19246), Parvimonas micra (ACTC 33270), Porphyromonas gingivalis (ATCC 33277), Prevotella intermedia (ATCC 25611), P. acnes (ATCC 6919), and F. nucleatum (NCTC 10562).

    Techniques:

    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces naeslundii (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: In vitro Increased Respiratory Activity of Selected Oral Bacteria May Explain Competitive and Collaborative Interactions in the Oral Microbiome

    doi: 10.3389/fcimb.2017.00235

    Figure Lengend Snippet: Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces naeslundii (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.

    Article Snippet: Bacterial cultures and collection The following strains commonly present in the oral microbiome (Aas et al., ; Maddi and Scannapieco, ; Loozen et al., ) were obtained from the American Type Culture Collection (ATCC): A . actinomycetemcomitans (ATCC 43718), F. nucleatum (ATCC 10953), P. gingivalis (ATCC 33277), P. intermedia (ATCC 25611), S. mutans (ATCC 25175), S. sobrinus (ATCC 33478), T. forsythia (ATCC 43037), Actinomyces naeslundii (ATCC 51655), Capnocytophaga sputigena (ATCC 33612), Streptococcus gordonii (ATCC 49818), Actinomyces viscosus (ATCC 15987), and S. mitis (ATCC 49456).

    Techniques: Activity Assay