Journal: PLoS ONE
Article Title: Effect of intravenous immunoglobulin on the function of Treg cells derived from immunosuppressed mice with Pseudomonas aeruginosa pneumonia
Figure Lengend Snippet: FOXP3 mRNA relative expression level in lung tissue in each group at 4, 8 and 24h. Six groups were studied: Control group, IS group, PA group, IS + PA group, PA + IVIG group and IS + PA + IVIG group. Immunosuppression was induced on day -5, -3 and -1 by intraperitoneal injection of CYP, PA pneumonia was induced on day 0 and IVIG mediated treatment models were immediately established following PA intervention on day 0. The left lung tissue of the mice was used for testing FOXP3 mRNA relative expression by qRT-PCR. qRT-PCR was carried out using a Thermo Fisher PCR kit with FOXP3 and GAPDH primer. FOXP3 mRNA relative expression level was calculated by the 2 (- ΔΔ CT) method. FOXP3 mRNA expression levels in lung tissue in six groups at 4, 8 and 24h were shown.
Article Snippet: Total cDNA was produced by reverse transcription via a Thermo Fisher reverse transcription kit (purchased from Thermo Fisher Scientific). qRT-PCR was carried out using a Thermo Fisher PCR kit with FOXP3 and glyceradehyde-3-phosphate-dehydrogenase (GAPDH) primers (synthesized by TaKaRa Biological Engineering Co., Ltd. Shanghai, China).
Techniques: Expressing, Injection, Mouse Assay, Quantitative RT-PCR, Polymerase Chain Reaction