Journal: PLoS ONE
Article Title: Codon Optimization, Expression in Escherichia coli, and Immunogenicity of Recombinant Chinese Sacbrood Virus (CSBV) Structural Proteins VP1, VP2, and VP3
Figure Lengend Snippet: Expression and purification of recombinant proteins. A, B, and C represent roVP1, roVP2, and roVP3 proteins, respectively. SDS-PAGE analysis of roVP1, roVP2, and roVP3 proteins were as follows: Lane 1, low molecular weight protein marker; Lane 2, pGEX-6P-1 vector after induction; Lane 3, wild type gene expression after induction; Lane 4, recoding gene expression after induction; Lane 5, purification of expressed proteins using GST-agarose affinity columns.
Article Snippet: E . coli strains DH5α and BL21(DE3) were purchased from TransGen Biotech (Beijing, China); the expression vector pGEX-6P-1 was from Invitrogen (California, USA); PCR premix, restriction enzymes, AMV reverse transcriptase XL, and DNA Ligation Kit were obtained from TaKaRa (Dalian, China); the SV Total RNA Isolation System, horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG were from Promega (Wisconsin, USA); the GST-Tagged Protein Purification Kit was from Clontech (New Jersey, USA); the BCA protein assay kit was from Sigma-Aldrich (Wisconsin, USA); GST(91G1) rabbit mAb was from Cell Signaling Technology (Boston, USA); rabbit anti-CSBV IgG was producted and stored by the authors’ laboratory; and HRP-conjugated rabbit anti-mice IgG was obtained from Abcam (London, UK).
Techniques: Expressing, Purification, Recombinant, SDS Page, Molecular Weight, Marker, Plasmid Preparation