expression vector pgex-6p-1 Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore pgex 6p 1 expression vector
    Pgex 6p 1 Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgex 6p 1 expression vector/product/Millipore
    Average 99 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    pgex 6p 1 expression vector - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    GE Healthcare expression vector pgex 6p 1
    Expression and purification of the rTgPuf1 Puf domain in E. coli . (A) Left panel shows a Coomassie Blue stained gel. Lane 1 and 2 shows the pellet and supernatant of lysates of induced <t>pGEX-6P-1-TgPuf1-PUM-HD/BL21.</t> Right panel is the immunoblot with anti-GST antibodies, which detected rTgPuf1 expression in uninduced (lane 3) and IPTG induced BL21 cells (lane 4). (B) Purification of rTgPuf1. Lane 1, lysate of induced BL21 cells; lane 2, lysate passed through a GST column; lane 3–7, elution with 50 mM Tris–HCl and 10 mM glutathione (pH 8.0).
    Expression Vector Pgex 6p 1, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 996 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expression vector pgex 6p 1/product/GE Healthcare
    Average 99 stars, based on 996 article reviews
    Price from $9.99 to $1999.99
    expression vector pgex 6p 1 - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    91
    Thermo Fisher expression vector pgex 6p 1
    Expression and purification of recombinant proteins. A, B, and C represent roVP1, roVP2, and roVP3 proteins, respectively. SDS-PAGE analysis of roVP1, roVP2, and roVP3 proteins were as follows: Lane 1, low molecular weight protein marker; Lane 2, <t>pGEX-6P-1</t> vector after induction; Lane 3, wild type gene expression after induction; Lane 4, recoding gene expression after induction; Lane 5, purification of expressed proteins using GST-agarose affinity columns.
    Expression Vector Pgex 6p 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expression vector pgex 6p 1/product/Thermo Fisher
    Average 91 stars, based on 112 article reviews
    Price from $9.99 to $1999.99
    expression vector pgex 6p 1 - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    Image Search Results


    Expression and purification of the rTgPuf1 Puf domain in E. coli . (A) Left panel shows a Coomassie Blue stained gel. Lane 1 and 2 shows the pellet and supernatant of lysates of induced pGEX-6P-1-TgPuf1-PUM-HD/BL21. Right panel is the immunoblot with anti-GST antibodies, which detected rTgPuf1 expression in uninduced (lane 3) and IPTG induced BL21 cells (lane 4). (B) Purification of rTgPuf1. Lane 1, lysate of induced BL21 cells; lane 2, lysate passed through a GST column; lane 3–7, elution with 50 mM Tris–HCl and 10 mM glutathione (pH 8.0).

    Journal: Parasites & Vectors

    Article Title: Characterization of TgPuf1, a member of the Puf family RNA-binding proteins from Toxoplasma gondii

    doi: 10.1186/1756-3305-7-141

    Figure Lengend Snippet: Expression and purification of the rTgPuf1 Puf domain in E. coli . (A) Left panel shows a Coomassie Blue stained gel. Lane 1 and 2 shows the pellet and supernatant of lysates of induced pGEX-6P-1-TgPuf1-PUM-HD/BL21. Right panel is the immunoblot with anti-GST antibodies, which detected rTgPuf1 expression in uninduced (lane 3) and IPTG induced BL21 cells (lane 4). (B) Purification of rTgPuf1. Lane 1, lysate of induced BL21 cells; lane 2, lysate passed through a GST column; lane 3–7, elution with 50 mM Tris–HCl and 10 mM glutathione (pH 8.0).

    Article Snippet: Expression of recombinant TgPuf1 Puf domain in Escherichia coli To express the conserved RNA-binding domain of TgPuf1 in bacteria, PCR was performed with T. gondii cDNA using two primers (CGGGATCC AGAAAAGGCGACTCAAAAG and ATAAGAATGCGGCCGC GTCACTGAAACCTGAGATG) designed to clone at the Bam HI and Not I sites of the expression vector pGEX-6P-1 (GE Healthcare).

    Techniques: Expressing, Purification, Staining

    Expression, purification, and verification of Blastocystis legumain. The Blastocystis legumain gene was inserted into pGEX-6p-1 and expressed in E. coli BL21(DE3) with induction of 0.5 m m isopropyl 1-thio-β- d -galactopyranoside at 16 °C

    Journal:

    Article Title: Blastocystis Legumain Is Localized on the Cell Surface, and Specific Inhibition of Its Activity Implicates a Pro-survival Role for the Enzyme *

    doi: 10.1074/jbc.M109.049064

    Figure Lengend Snippet: Expression, purification, and verification of Blastocystis legumain. The Blastocystis legumain gene was inserted into pGEX-6p-1 and expressed in E. coli BL21(DE3) with induction of 0.5 m m isopropyl 1-thio-β- d -galactopyranoside at 16 °C

    Article Snippet: These primers contained BamHI and XhoI restriction sites to allow directional cloning into E. coli expression vector pGEX-6p-1 (GE Healthcare). pGEX-6p-1 containing the Blastocystis legumain gene was transformed into E. coli BL21(DE3) and amplified in 500 ml of LB containing 100 μg ml−1 ampicillin at 37 °C and 200 rpm.

    Techniques: Expressing, Purification

    Expression and purification of recombinant proteins. A, B, and C represent roVP1, roVP2, and roVP3 proteins, respectively. SDS-PAGE analysis of roVP1, roVP2, and roVP3 proteins were as follows: Lane 1, low molecular weight protein marker; Lane 2, pGEX-6P-1 vector after induction; Lane 3, wild type gene expression after induction; Lane 4, recoding gene expression after induction; Lane 5, purification of expressed proteins using GST-agarose affinity columns.

    Journal: PLoS ONE

    Article Title: Codon Optimization, Expression in Escherichia coli, and Immunogenicity of Recombinant Chinese Sacbrood Virus (CSBV) Structural Proteins VP1, VP2, and VP3

    doi: 10.1371/journal.pone.0128486

    Figure Lengend Snippet: Expression and purification of recombinant proteins. A, B, and C represent roVP1, roVP2, and roVP3 proteins, respectively. SDS-PAGE analysis of roVP1, roVP2, and roVP3 proteins were as follows: Lane 1, low molecular weight protein marker; Lane 2, pGEX-6P-1 vector after induction; Lane 3, wild type gene expression after induction; Lane 4, recoding gene expression after induction; Lane 5, purification of expressed proteins using GST-agarose affinity columns.

    Article Snippet: E . coli strains DH5α and BL21(DE3) were purchased from TransGen Biotech (Beijing, China); the expression vector pGEX-6P-1 was from Invitrogen (California, USA); PCR premix, restriction enzymes, AMV reverse transcriptase XL, and DNA Ligation Kit were obtained from TaKaRa (Dalian, China); the SV Total RNA Isolation System, horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG were from Promega (Wisconsin, USA); the GST-Tagged Protein Purification Kit was from Clontech (New Jersey, USA); the BCA protein assay kit was from Sigma-Aldrich (Wisconsin, USA); GST(91G1) rabbit mAb was from Cell Signaling Technology (Boston, USA); rabbit anti-CSBV IgG was producted and stored by the authors’ laboratory; and HRP-conjugated rabbit anti-mice IgG was obtained from Abcam (London, UK).

    Techniques: Expressing, Purification, Recombinant, SDS Page, Molecular Weight, Marker, Plasmid Preparation