Journal: Journal of Bacteriology
Article Title: Putative Exopolysaccharide Synthesis Genes Influence Pseudomonas aeruginosa Biofilm Development
Figure Lengend Snippet: Analysis of the PA2231-2245 gene cluster by RT-PCR of intergenic regions. Lane 1, size markers; lane 2, PCR amplification product of PA2230-2231 cluster with genomic DNA as the template (this serves as a control for RT-PCR of this region); lane 3, RT-PCR of PA2230 and -2231 (the lack of product indicates that PA2230 and -2231 are not cotranscribed); lane 4, size marker; lane 5, RT-PCR of PA2231 and -2232; lane 6, PA2232 and -2233; lane 7, PA2233 and -2234; lane 8, PA2234 and -2235; lane 9, PA2235 and -2236; lane 10, PA2236 and -2237; lane 11, PA2237 and -2238; lane 12, PA2238 and -2239; lane 13, PA2239 and -2240; lane 14, PA2240 and -2241; lane 15, PA2241 and -2242; lane 16, PA2242 and -2243; lane 17, PA2243 and -2244; lane 18, PA2244 and -2245. Control experiments without reverse transcriptase reactions prior to PCR were negative.
Article Snippet: DNA contamination of the purified RNA was assessed by Expand long-template PCR using rplU primers.
Techniques: Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Amplification, Marker