exonuclease iii reaction buffer Search Results


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  • 99
    New England Biolabs exonuclease iii reaction buffer
    Exonuclease Iii Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exonuclease iii reaction buffer/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
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    Thermo Fisher exonuclease iii reaction buffer
    Exonuclease Iii Reaction Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exonuclease iii reaction buffer/product/Thermo Fisher
    Average 99 stars, based on 5 article reviews
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    94
    Thermo Fisher exonuclease iii
    Effect of fixation protocol on the signal intensity. The results of the detection of mycoplasmas’ <t>DNA</t> in samples with Lep cells accidentally infected with mycoplasma (RT-PCR-positive, MycoAlert-positive) using the developed approach and <t>three</t> fixation protocols is shown. The samples were fixed either with formaldehyde, with 70% ethanol, or in a solution of acetic acid and methanol (1:3). The enzymatic mixture contained biotin-dUTP. Biotin-dUTP was detected by indirect immunofluorescence (green). The overall DNA was labeled by DAPI (blue), mitochondria were labeled with the mitochondrial marker MTCO2 (red). The images were acquired for 16.56 ms (biotin-derived signal); 2.636 ms (DAPI) and 7.916 ms (MTCO2-derived signal). Scale bar = 10 µm.
    Exonuclease Iii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 292 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    exonuclease iii - by Bioz Stars, 2020-07
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    93
    Promega exonuclease iii
    Effect of fixation protocol on the signal intensity. The results of the detection of mycoplasmas’ <t>DNA</t> in samples with Lep cells accidentally infected with mycoplasma (RT-PCR-positive, MycoAlert-positive) using the developed approach and <t>three</t> fixation protocols is shown. The samples were fixed either with formaldehyde, with 70% ethanol, or in a solution of acetic acid and methanol (1:3). The enzymatic mixture contained biotin-dUTP. Biotin-dUTP was detected by indirect immunofluorescence (green). The overall DNA was labeled by DAPI (blue), mitochondria were labeled with the mitochondrial marker MTCO2 (red). The images were acquired for 16.56 ms (biotin-derived signal); 2.636 ms (DAPI) and 7.916 ms (MTCO2-derived signal). Scale bar = 10 µm.
    Exonuclease Iii, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 596 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exonuclease iii/product/Promega
    Average 93 stars, based on 596 article reviews
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    exonuclease iii - by Bioz Stars, 2020-07
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    94
    Millipore exo iii reaction buffer
    Effect of fixation protocol on the signal intensity. The results of the detection of mycoplasmas’ <t>DNA</t> in samples with Lep cells accidentally infected with mycoplasma (RT-PCR-positive, MycoAlert-positive) using the developed approach and <t>three</t> fixation protocols is shown. The samples were fixed either with formaldehyde, with 70% ethanol, or in a solution of acetic acid and methanol (1:3). The enzymatic mixture contained biotin-dUTP. Biotin-dUTP was detected by indirect immunofluorescence (green). The overall DNA was labeled by DAPI (blue), mitochondria were labeled with the mitochondrial marker MTCO2 (red). The images were acquired for 16.56 ms (biotin-derived signal); 2.636 ms (DAPI) and 7.916 ms (MTCO2-derived signal). Scale bar = 10 µm.
    Exo Iii Reaction Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs i iiic
    C-technology application and preliminary assessment in Hi-C. ( A ) DNA library (572 ng) linear noise elimination for C-technologies, after proximal-ligation. M (left), 1 kb DNA ladder; 1: LRL, LRL used for linear noise elimination. 2: LRC, LRC used for linear noise elimination; 3: <t>LIC,</t> LIC used for linear noise elimination; 4: <t>I+IIIC,</t> I+IIIC used for linear noise elimination; C: Co, without exonucleases digestion; M (right): 100 bp DNA ladder. ( B ) The yield ratio of remaining DNA after four linear elimination treatments, which was consistent to (A). The remaining ratio (gray) and eliminated ratio (purple) are shown together in the bar chart. ( C ) A 40 ng library was given four exonuclease treatment combinations, along with addition of plasmid chaperon carrier, as indicated. M: 1 kb + 100 bp DNA ladder. 1′: LRLP1, LRL with P1 chaperon used for linear noise elimination; 2′: LRC with P1 chaperon used for linear noise elimination; 3′: LICP1, LIC with P1 chaperon used for linear noise elimination; 4′: I+IIICP1, I+IIIC with P1 chaperon used for linear noise elimination; C’: P1 chaperon added, without exonucleases digestion. ( D ) The yield ratio of remaining DNA, after four linear DNA elimination treatments, which was consistent to ( C ). ( E ) HiC-Pro filtering results of Hi-C with exonuclease elimination LIC, compared with standard Hi-C in Figure 1B . LIC-Hi-C had three experimental replicates (LIC-Hi-C 1; LIC-Hi-C 2; and LIC-Hi-C 3). HiC-Pro terms are as indicated in Supplemental S.3.
    I Iiic, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/i iiic/product/New England Biolabs
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    93
    Millipore t4 dna polymerase buffer
    C-technology application and preliminary assessment in Hi-C. ( A ) DNA library (572 ng) linear noise elimination for C-technologies, after proximal-ligation. M (left), 1 kb DNA ladder; 1: LRL, LRL used for linear noise elimination. 2: LRC, LRC used for linear noise elimination; 3: <t>LIC,</t> LIC used for linear noise elimination; 4: <t>I+IIIC,</t> I+IIIC used for linear noise elimination; C: Co, without exonucleases digestion; M (right): 100 bp DNA ladder. ( B ) The yield ratio of remaining DNA after four linear elimination treatments, which was consistent to (A). The remaining ratio (gray) and eliminated ratio (purple) are shown together in the bar chart. ( C ) A 40 ng library was given four exonuclease treatment combinations, along with addition of plasmid chaperon carrier, as indicated. M: 1 kb + 100 bp DNA ladder. 1′: LRLP1, LRL with P1 chaperon used for linear noise elimination; 2′: LRC with P1 chaperon used for linear noise elimination; 3′: LICP1, LIC with P1 chaperon used for linear noise elimination; 4′: I+IIICP1, I+IIIC with P1 chaperon used for linear noise elimination; C’: P1 chaperon added, without exonucleases digestion. ( D ) The yield ratio of remaining DNA, after four linear DNA elimination treatments, which was consistent to ( C ). ( E ) HiC-Pro filtering results of Hi-C with exonuclease elimination LIC, compared with standard Hi-C in Figure 1B . LIC-Hi-C had three experimental replicates (LIC-Hi-C 1; LIC-Hi-C 2; and LIC-Hi-C 3). HiC-Pro terms are as indicated in Supplemental S.3.
    T4 Dna Polymerase Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna polymerase buffer/product/Millipore
    Average 93 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    t4 dna polymerase buffer - by Bioz Stars, 2020-07
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    Image Search Results


    Effect of fixation protocol on the signal intensity. The results of the detection of mycoplasmas’ DNA in samples with Lep cells accidentally infected with mycoplasma (RT-PCR-positive, MycoAlert-positive) using the developed approach and three fixation protocols is shown. The samples were fixed either with formaldehyde, with 70% ethanol, or in a solution of acetic acid and methanol (1:3). The enzymatic mixture contained biotin-dUTP. Biotin-dUTP was detected by indirect immunofluorescence (green). The overall DNA was labeled by DAPI (blue), mitochondria were labeled with the mitochondrial marker MTCO2 (red). The images were acquired for 16.56 ms (biotin-derived signal); 2.636 ms (DAPI) and 7.916 ms (MTCO2-derived signal). Scale bar = 10 µm.

    Journal: Cells

    Article Title: A New Sensitive Method for the Detection of Mycoplasmas Using Fluorescence Microscopy

    doi: 10.3390/cells8121510

    Figure Lengend Snippet: Effect of fixation protocol on the signal intensity. The results of the detection of mycoplasmas’ DNA in samples with Lep cells accidentally infected with mycoplasma (RT-PCR-positive, MycoAlert-positive) using the developed approach and three fixation protocols is shown. The samples were fixed either with formaldehyde, with 70% ethanol, or in a solution of acetic acid and methanol (1:3). The enzymatic mixture contained biotin-dUTP. Biotin-dUTP was detected by indirect immunofluorescence (green). The overall DNA was labeled by DAPI (blue), mitochondria were labeled with the mitochondrial marker MTCO2 (red). The images were acquired for 16.56 ms (biotin-derived signal); 2.636 ms (DAPI) and 7.916 ms (MTCO2-derived signal). Scale bar = 10 µm.

    Article Snippet: In this case, the nuclease solution contained DNase I (1 U/µL, the working concentration was 100 U/mL; one unit of DNase I completely degrades 1 µg of plasmid DNA in 10 min at 37 °C, ThermoFisher Scientific, EN0525), Exonuclease III (200 U/µL, the working concentration was 4 U/µL; one unit of Exonuclease III catalyzes the release of 1 nmol of acid soluble reaction products from E. coli [3 H]-DNA in 30 min at 37 °C, ThermoFisher Scientific, EN0191), 1× buffer for Exonuclease III (ThermoFisher Scientific) and 0.1 mM CaCl2 .

    Techniques: Infection, Reverse Transcription Polymerase Chain Reaction, Immunofluorescence, Labeling, Marker, Mass Spectrometry, Derivative Assay

    C-technology application and preliminary assessment in Hi-C. ( A ) DNA library (572 ng) linear noise elimination for C-technologies, after proximal-ligation. M (left), 1 kb DNA ladder; 1: LRL, LRL used for linear noise elimination. 2: LRC, LRC used for linear noise elimination; 3: LIC, LIC used for linear noise elimination; 4: I+IIIC, I+IIIC used for linear noise elimination; C: Co, without exonucleases digestion; M (right): 100 bp DNA ladder. ( B ) The yield ratio of remaining DNA after four linear elimination treatments, which was consistent to (A). The remaining ratio (gray) and eliminated ratio (purple) are shown together in the bar chart. ( C ) A 40 ng library was given four exonuclease treatment combinations, along with addition of plasmid chaperon carrier, as indicated. M: 1 kb + 100 bp DNA ladder. 1′: LRLP1, LRL with P1 chaperon used for linear noise elimination; 2′: LRC with P1 chaperon used for linear noise elimination; 3′: LICP1, LIC with P1 chaperon used for linear noise elimination; 4′: I+IIICP1, I+IIIC with P1 chaperon used for linear noise elimination; C’: P1 chaperon added, without exonucleases digestion. ( D ) The yield ratio of remaining DNA, after four linear DNA elimination treatments, which was consistent to ( C ). ( E ) HiC-Pro filtering results of Hi-C with exonuclease elimination LIC, compared with standard Hi-C in Figure 1B . LIC-Hi-C had three experimental replicates (LIC-Hi-C 1; LIC-Hi-C 2; and LIC-Hi-C 3). HiC-Pro terms are as indicated in Supplemental S.3.

    Journal: Nucleic Acids Research

    Article Title: Exonuclease combinations reduce noises in 3D genomics technologies

    doi: 10.1093/nar/gkaa106

    Figure Lengend Snippet: C-technology application and preliminary assessment in Hi-C. ( A ) DNA library (572 ng) linear noise elimination for C-technologies, after proximal-ligation. M (left), 1 kb DNA ladder; 1: LRL, LRL used for linear noise elimination. 2: LRC, LRC used for linear noise elimination; 3: LIC, LIC used for linear noise elimination; 4: I+IIIC, I+IIIC used for linear noise elimination; C: Co, without exonucleases digestion; M (right): 100 bp DNA ladder. ( B ) The yield ratio of remaining DNA after four linear elimination treatments, which was consistent to (A). The remaining ratio (gray) and eliminated ratio (purple) are shown together in the bar chart. ( C ) A 40 ng library was given four exonuclease treatment combinations, along with addition of plasmid chaperon carrier, as indicated. M: 1 kb + 100 bp DNA ladder. 1′: LRLP1, LRL with P1 chaperon used for linear noise elimination; 2′: LRC with P1 chaperon used for linear noise elimination; 3′: LICP1, LIC with P1 chaperon used for linear noise elimination; 4′: I+IIICP1, I+IIIC with P1 chaperon used for linear noise elimination; C’: P1 chaperon added, without exonucleases digestion. ( D ) The yield ratio of remaining DNA, after four linear DNA elimination treatments, which was consistent to ( C ). ( E ) HiC-Pro filtering results of Hi-C with exonuclease elimination LIC, compared with standard Hi-C in Figure 1B . LIC-Hi-C had three experimental replicates (LIC-Hi-C 1; LIC-Hi-C 2; and LIC-Hi-C 3). HiC-Pro terms are as indicated in Supplemental S.3.

    Article Snippet: After LRL (Lambda and RecJF within Lambda buffer) (NEB), LRC (Lambda and RecJF within Cutsmart buffer) (NEB), LIC (Lambda and Exonuclease I within Cutsmart buffer) (NEB) and I+IIIC (I+III or IIIIC) (Exonuclease I and Exonuclease III within Cutsmart buffer) (NEB) elimination, qPCR was performed.

    Techniques: Hi-C, Ligation, Plasmid Preparation