exonuclease i digestion Search Results


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  • 99
    New England Biolabs exonuclease digestion
    Exonuclease Digestion, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher exonuclease i digestion
    Increased TCRα sJ locus CE accumulation in Atm −/− thymocytes. (A) Southern analysis strategy showing the relative positions of the NsiI (N) and StuI (S) sites, PS6, and the CαI probe. Fragment sizes generated by germline TCRα sJ alleles and Jα56 and Jα61 CEs are indicated. The schematics are not drawn to scale. (B) Southern blot analyses of total thymocyte DNA and kidney DNA (K) digested with either NsiI or HincII and hybridized to the CαI probe or PS6. Bands corresponding to Jα61 and Jα56 CEs are indicated (arrows). The band corresponding to the germline fragment is indicated by an asterisk. Hybridization of StuI and NsiI digested DNA to PR2 is shown as a DNA loading control. The molecular mass markers (in kb) are indicated. (C) LMPCR analysis of Jα56 and Jα61 CEs, as described in Materials and methods. Shown are fivefold serial dilutions of linker-ligated samples. IL-2 gene PCR is also shown as a DNA loading control. (D and E) <t>Exonuclease</t> V sensitivity of Jα56 CEs was assayed by hybridizing the membrane from Fig. 3D to the CαI probe. Bands corresponding to the Jα61 and Jα56 CEs are indicated (arrows). The band corresponding to the germline TCRα sJ allele is indicated by an asterisk. The positions of the molecular mass markers (in kb) are indicated (D). Retention of the Jα56 CE band in exonuclease V–treated samples was quantified as described in Materials and methods (E). (F and G) TCRα sJ/sJ :Atm − / − thymocyte DNA was treated with mung bean nuclease followed by exonuclease V before digestion with StuI and hybridization to the CαI probe, as described in Materials and methods. Fragments corresponding to Jα61 and Jα56 CEs are indicated (arrows). The fragment corresponding to the germline TCRα sJ allele is indicated by an asterisk (F). Retention of the Jα56 CE band in samples treated with mung bean nuclease and exonuclease V was quantified as described in Materials and methods (G).
    Exonuclease I Digestion, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exonuclease i digestion/product/Thermo Fisher
    Average 99 stars, based on 42 article reviews
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    94
    Millipore exonuclease digestion
    Increased TCRα sJ locus CE accumulation in Atm −/− thymocytes. (A) Southern analysis strategy showing the relative positions of the NsiI (N) and StuI (S) sites, PS6, and the CαI probe. Fragment sizes generated by germline TCRα sJ alleles and Jα56 and Jα61 CEs are indicated. The schematics are not drawn to scale. (B) Southern blot analyses of total thymocyte DNA and kidney DNA (K) digested with either NsiI or HincII and hybridized to the CαI probe or PS6. Bands corresponding to Jα61 and Jα56 CEs are indicated (arrows). The band corresponding to the germline fragment is indicated by an asterisk. Hybridization of StuI and NsiI digested DNA to PR2 is shown as a DNA loading control. The molecular mass markers (in kb) are indicated. (C) LMPCR analysis of Jα56 and Jα61 CEs, as described in Materials and methods. Shown are fivefold serial dilutions of linker-ligated samples. IL-2 gene PCR is also shown as a DNA loading control. (D and E) <t>Exonuclease</t> V sensitivity of Jα56 CEs was assayed by hybridizing the membrane from Fig. 3D to the CαI probe. Bands corresponding to the Jα61 and Jα56 CEs are indicated (arrows). The band corresponding to the germline TCRα sJ allele is indicated by an asterisk. The positions of the molecular mass markers (in kb) are indicated (D). Retention of the Jα56 CE band in exonuclease V–treated samples was quantified as described in Materials and methods (E). (F and G) TCRα sJ/sJ :Atm − / − thymocyte DNA was treated with mung bean nuclease followed by exonuclease V before digestion with StuI and hybridization to the CαI probe, as described in Materials and methods. Fragments corresponding to Jα61 and Jα56 CEs are indicated (arrows). The fragment corresponding to the germline TCRα sJ allele is indicated by an asterisk (F). Retention of the Jα56 CE band in samples treated with mung bean nuclease and exonuclease V was quantified as described in Materials and methods (G).
    Exonuclease Digestion, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher exoi sap enzymatic digestion
    Increased TCRα sJ locus CE accumulation in Atm −/− thymocytes. (A) Southern analysis strategy showing the relative positions of the NsiI (N) and StuI (S) sites, PS6, and the CαI probe. Fragment sizes generated by germline TCRα sJ alleles and Jα56 and Jα61 CEs are indicated. The schematics are not drawn to scale. (B) Southern blot analyses of total thymocyte DNA and kidney DNA (K) digested with either NsiI or HincII and hybridized to the CαI probe or PS6. Bands corresponding to Jα61 and Jα56 CEs are indicated (arrows). The band corresponding to the germline fragment is indicated by an asterisk. Hybridization of StuI and NsiI digested DNA to PR2 is shown as a DNA loading control. The molecular mass markers (in kb) are indicated. (C) LMPCR analysis of Jα56 and Jα61 CEs, as described in Materials and methods. Shown are fivefold serial dilutions of linker-ligated samples. IL-2 gene PCR is also shown as a DNA loading control. (D and E) <t>Exonuclease</t> V sensitivity of Jα56 CEs was assayed by hybridizing the membrane from Fig. 3D to the CαI probe. Bands corresponding to the Jα61 and Jα56 CEs are indicated (arrows). The band corresponding to the germline TCRα sJ allele is indicated by an asterisk. The positions of the molecular mass markers (in kb) are indicated (D). Retention of the Jα56 CE band in exonuclease V–treated samples was quantified as described in Materials and methods (E). (F and G) TCRα sJ/sJ :Atm − / − thymocyte DNA was treated with mung bean nuclease followed by exonuclease V before digestion with StuI and hybridization to the CαI probe, as described in Materials and methods. Fragments corresponding to Jα61 and Jα56 CEs are indicated (arrows). The fragment corresponding to the germline TCRα sJ allele is indicated by an asterisk (F). Retention of the Jα56 CE band in samples treated with mung bean nuclease and exonuclease V was quantified as described in Materials and methods (G).
    Exoi Sap Enzymatic Digestion, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa exonuclease iii digestion
    The distal region of the IL-6 promoter contains functional C/EBP motifs A. IL-6 promoter Luciferase activity in PC3 cells transfected with the <t>pGL2-IL-6</t> deletion mutants shown on the left and pRV with and without treatment with rGal-3BP (2.5 µg/ml) for 24 hours. The data represent the mean (±SD) ratio Firefly Luciferase/Renilla Luciferase and are representative of one among <t>three</t> separate experiments each performed in triplicate samples. B. Sequencing analysis of the region of the IL-6 promoter extending from position −1586 to −1255 showing the presence of 3 C/EBP domains. C. EMSA of nuclear extracts from PC3 cells treated with rGAL-3BP (5 µg/ml) using biotinylated oligonucleotides in the presence of 10× fold excess of non-biotinylated oligonucleotides when indicated.
    Exonuclease Iii Digestion, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Increased TCRα sJ locus CE accumulation in Atm −/− thymocytes. (A) Southern analysis strategy showing the relative positions of the NsiI (N) and StuI (S) sites, PS6, and the CαI probe. Fragment sizes generated by germline TCRα sJ alleles and Jα56 and Jα61 CEs are indicated. The schematics are not drawn to scale. (B) Southern blot analyses of total thymocyte DNA and kidney DNA (K) digested with either NsiI or HincII and hybridized to the CαI probe or PS6. Bands corresponding to Jα61 and Jα56 CEs are indicated (arrows). The band corresponding to the germline fragment is indicated by an asterisk. Hybridization of StuI and NsiI digested DNA to PR2 is shown as a DNA loading control. The molecular mass markers (in kb) are indicated. (C) LMPCR analysis of Jα56 and Jα61 CEs, as described in Materials and methods. Shown are fivefold serial dilutions of linker-ligated samples. IL-2 gene PCR is also shown as a DNA loading control. (D and E) Exonuclease V sensitivity of Jα56 CEs was assayed by hybridizing the membrane from Fig. 3D to the CαI probe. Bands corresponding to the Jα61 and Jα56 CEs are indicated (arrows). The band corresponding to the germline TCRα sJ allele is indicated by an asterisk. The positions of the molecular mass markers (in kb) are indicated (D). Retention of the Jα56 CE band in exonuclease V–treated samples was quantified as described in Materials and methods (E). (F and G) TCRα sJ/sJ :Atm − / − thymocyte DNA was treated with mung bean nuclease followed by exonuclease V before digestion with StuI and hybridization to the CαI probe, as described in Materials and methods. Fragments corresponding to Jα61 and Jα56 CEs are indicated (arrows). The fragment corresponding to the germline TCRα sJ allele is indicated by an asterisk (F). Retention of the Jα56 CE band in samples treated with mung bean nuclease and exonuclease V was quantified as described in Materials and methods (G).

    Journal: The Journal of Experimental Medicine

    Article Title: Defects in coding joint formation in vivo in developing ATM-deficient B and T lymphocytes

    doi: 10.1084/jem.20061460

    Figure Lengend Snippet: Increased TCRα sJ locus CE accumulation in Atm −/− thymocytes. (A) Southern analysis strategy showing the relative positions of the NsiI (N) and StuI (S) sites, PS6, and the CαI probe. Fragment sizes generated by germline TCRα sJ alleles and Jα56 and Jα61 CEs are indicated. The schematics are not drawn to scale. (B) Southern blot analyses of total thymocyte DNA and kidney DNA (K) digested with either NsiI or HincII and hybridized to the CαI probe or PS6. Bands corresponding to Jα61 and Jα56 CEs are indicated (arrows). The band corresponding to the germline fragment is indicated by an asterisk. Hybridization of StuI and NsiI digested DNA to PR2 is shown as a DNA loading control. The molecular mass markers (in kb) are indicated. (C) LMPCR analysis of Jα56 and Jα61 CEs, as described in Materials and methods. Shown are fivefold serial dilutions of linker-ligated samples. IL-2 gene PCR is also shown as a DNA loading control. (D and E) Exonuclease V sensitivity of Jα56 CEs was assayed by hybridizing the membrane from Fig. 3D to the CαI probe. Bands corresponding to the Jα61 and Jα56 CEs are indicated (arrows). The band corresponding to the germline TCRα sJ allele is indicated by an asterisk. The positions of the molecular mass markers (in kb) are indicated (D). Retention of the Jα56 CE band in exonuclease V–treated samples was quantified as described in Materials and methods (E). (F and G) TCRα sJ/sJ :Atm − / − thymocyte DNA was treated with mung bean nuclease followed by exonuclease V before digestion with StuI and hybridization to the CαI probe, as described in Materials and methods. Fragments corresponding to Jα61 and Jα56 CEs are indicated (arrows). The fragment corresponding to the germline TCRα sJ allele is indicated by an asterisk (F). Retention of the Jα56 CE band in samples treated with mung bean nuclease and exonuclease V was quantified as described in Materials and methods (G).

    Article Snippet: For exonuclease V digestion, 15 μg of thymocyte DNA was treated with increasing concentrations of exonuclease V, using the manufacturer's buffer conditions (USB Corporation), for 1 h at 37°C before restriction enzyme digestion and Southern blot analyses.

    Techniques: Generated, Southern Blot, Hybridization, Polymerase Chain Reaction

    Equivalent TCRα sJ locus SE accumulation in Atm +/+ and Atm −/− thymocytes. (A) Schematics of the wild-type TCRα + and TCRα sJ loci. The Vα (open rectangles) and Jα (closed rectangles) gene segments are shown, as is the TCRα constant region gene (Cα; gray rectangles). The number in parenthesis indicates the number of Jα segments in the locus. (B) Southern blot analysis strategy. The relative positions of the HincII (H) and StuI (S) sites and P8 are shown. Fragment sizes generated by germline TCRα sJ alleles and Jα56 and Jα61 SEs are indicated. The schematics are not drawn to scale. (C) Southern blot analyses of total thymocyte DNA and kidney DNA (K) digested with either StuI or HincII and hybridized to P8. Hybridization to PR2 is shown as a DNA loading control. The fragment corresponding to the germline TCRα sJ allele is indicated by the asterisk. The fragments corresponding to the Jα61 SE are also indicated (arrows). The molecular mass markers (in kb) are indicated. (D and E) Thymocyte genomic DNA was digested in the absence (−) or in the presence of increasing concentrations of exonuclease V (ExoV) before digestions with StuI and hybridization to P8 (D). The fragment corresponding to the germline TCRα sJ allele is indicated by the asterisk. Retention of the Jα61 SE band (arrow) in exonuclease V–treated samples was quantified as described in Materials and methods (E). (F) LMPCR analysis of Jα56 and Jα61 SEs, as described in the Materials and methods. Shown are fivefold serial dilutions of linker-ligated samples, as well as the no-ligase control (−). IL-2 gene PCR is also shown as a DNA loading control.

    Journal: The Journal of Experimental Medicine

    Article Title: Defects in coding joint formation in vivo in developing ATM-deficient B and T lymphocytes

    doi: 10.1084/jem.20061460

    Figure Lengend Snippet: Equivalent TCRα sJ locus SE accumulation in Atm +/+ and Atm −/− thymocytes. (A) Schematics of the wild-type TCRα + and TCRα sJ loci. The Vα (open rectangles) and Jα (closed rectangles) gene segments are shown, as is the TCRα constant region gene (Cα; gray rectangles). The number in parenthesis indicates the number of Jα segments in the locus. (B) Southern blot analysis strategy. The relative positions of the HincII (H) and StuI (S) sites and P8 are shown. Fragment sizes generated by germline TCRα sJ alleles and Jα56 and Jα61 SEs are indicated. The schematics are not drawn to scale. (C) Southern blot analyses of total thymocyte DNA and kidney DNA (K) digested with either StuI or HincII and hybridized to P8. Hybridization to PR2 is shown as a DNA loading control. The fragment corresponding to the germline TCRα sJ allele is indicated by the asterisk. The fragments corresponding to the Jα61 SE are also indicated (arrows). The molecular mass markers (in kb) are indicated. (D and E) Thymocyte genomic DNA was digested in the absence (−) or in the presence of increasing concentrations of exonuclease V (ExoV) before digestions with StuI and hybridization to P8 (D). The fragment corresponding to the germline TCRα sJ allele is indicated by the asterisk. Retention of the Jα61 SE band (arrow) in exonuclease V–treated samples was quantified as described in Materials and methods (E). (F) LMPCR analysis of Jα56 and Jα61 SEs, as described in the Materials and methods. Shown are fivefold serial dilutions of linker-ligated samples, as well as the no-ligase control (−). IL-2 gene PCR is also shown as a DNA loading control.

    Article Snippet: For exonuclease V digestion, 15 μg of thymocyte DNA was treated with increasing concentrations of exonuclease V, using the manufacturer's buffer conditions (USB Corporation), for 1 h at 37°C before restriction enzyme digestion and Southern blot analyses.

    Techniques: Southern Blot, Generated, Hybridization, Polymerase Chain Reaction

    The distal region of the IL-6 promoter contains functional C/EBP motifs A. IL-6 promoter Luciferase activity in PC3 cells transfected with the pGL2-IL-6 deletion mutants shown on the left and pRV with and without treatment with rGal-3BP (2.5 µg/ml) for 24 hours. The data represent the mean (±SD) ratio Firefly Luciferase/Renilla Luciferase and are representative of one among three separate experiments each performed in triplicate samples. B. Sequencing analysis of the region of the IL-6 promoter extending from position −1586 to −1255 showing the presence of 3 C/EBP domains. C. EMSA of nuclear extracts from PC3 cells treated with rGAL-3BP (5 µg/ml) using biotinylated oligonucleotides in the presence of 10× fold excess of non-biotinylated oligonucleotides when indicated.

    Journal: Cancer research

    Article Title: A GALECTIN-3-DEPENDENT PATHWAY UPREGULATES INTERLEUKIN-6 IN THE MICROENVIRONMENT OF HUMAN NEUROBLASTOMA

    doi: 10.1158/0008-5472.CAN-11-2165

    Figure Lengend Snippet: The distal region of the IL-6 promoter contains functional C/EBP motifs A. IL-6 promoter Luciferase activity in PC3 cells transfected with the pGL2-IL-6 deletion mutants shown on the left and pRV with and without treatment with rGal-3BP (2.5 µg/ml) for 24 hours. The data represent the mean (±SD) ratio Firefly Luciferase/Renilla Luciferase and are representative of one among three separate experiments each performed in triplicate samples. B. Sequencing analysis of the region of the IL-6 promoter extending from position −1586 to −1255 showing the presence of 3 C/EBP domains. C. EMSA of nuclear extracts from PC3 cells treated with rGAL-3BP (5 µg/ml) using biotinylated oligonucleotides in the presence of 10× fold excess of non-biotinylated oligonucleotides when indicated.

    Article Snippet: IL-6 promoter deletion mutants in the pGL2-IL-6-Luc construct were generated either by Exonuclease III digestion using the Deletion Kit for kilo sequencing (Takara) in accordance with the instructions of the manufacturer (deletion −1041) or by restriction endonuclease digestion with KpnI and NheI (deletion mutant −212) followed by Klenow fragment reaction at 37°C for 15 minutes.

    Techniques: Functional Assay, Luciferase, Activity Assay, Transfection, Sequencing