Journal: Pharmaceutics

Article Title: Structural Adaptation of the Single-Stranded DNA-Binding Protein C-Terminal to DNA Metabolizing Partners Guides Inhibitor Design

doi: 10.3390/pharmaceutics15041032

Figure Lengend Snippet: ( A ) Domain structure of E. coli SSB protein. ( B ) Structure of E. coli single-stranded-DNA binding protein as a tetramer (PDB structure 4MZ9). The IDP regions are red lines, and the acidic tips are shown as amino acid sequences. ( C ) SSB-Ct sequence and its numbering as a segment of the whole protein. wtSSB-Ct is an octapeptide with a sequence identical to the SSB-Ct segment. Sequences are colored according to the conservation scores (see lower part of panel ( C ), * denotes the highest score) obtained from PRALINE .

Article Snippet: pET14b— E. coli Exonuclease I was a gift from Charles Bell (Addgene plasmid # 104552; http://n2t.net/addgene:104552 ; RRID: Addgene_104552, accessed on 23 July 2019).

Techniques: Binding Assay, Sequencing

Journal: World Journal of Diabetes

Article Title: Bone marrow-derived mesenchymal stem cell-derived exosome-loaded miR-129-5p targets high-mobility group box 1 attenuates neurological-impairment after diabetic cerebral hemorrhage

doi: 10.4239/wjd.v15.i9.1979

Figure Lengend Snippet: Bone marrow-derived mesenchymal stem cell-derived exosome attenuated neurological deficits in vivo . A: Dil-labeled bone marrow-derived mesenchymal stem cell-derived exosomes (BMSC-exo) were injected in the tail vein of mice; B: Microglia (green) were labeled with ionized calcium-binding adaptor molecule 1 to observe the phagocytosis of BMSC-exo (red) by microglia. Scale bar = 50 μm; C: Immunofluorescence staining to observe the location of immunoglobulin G expression. Scale bar = 100 μm; D: Brain slices and hematoma volume were analyzed based on T2 magnetic resonance imaging. Experimental data are expressed as mean ± SD. a P < 0.05, intracerebral hemorrhage (ICH) + diabetes mellitus (DM) group vs ICH + DM + BMSC-exo group, t -test; E: Brain water content analysis. Experimental data are expressed as mean ± SD. a P < 0.01, ICH + DM group vs ICH + DM + BMSC-exo group, t -test; F: Neurological function scores and balance beam scores. Experimental data are expressed as mean ± SD. a P < 0.05, ICH + DM group vs ICH + DM + BMSC-exo group, t -test; G: The blood glucose levels of mice in each group were detected, and it was found that BMSC-exo could not reduce the blood glucose levels after diabetic cerebral hemorrhage in mice. Con: Control; BMSC-exo: Bone marrow mesenchymal stem cell derived exosomes; ICH: Intracerebral hemorrhage; DM: Diabetes mellitus; IgG: Immunoglobulin G.

Article Snippet: Then, 50 μL exo-red mixture (MedChemExpress, Monmouth Junction, NJ, United States) was added to the resuspended BMSC-exo, and the samples were incubated for 10 minutes at 37 °C.

Techniques: Derivative Assay, In Vivo, Labeling, Injection, Binding Assay, Immunofluorescence, Staining, Expressing, Magnetic Resonance Imaging, Control