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  • 99
    Thermo Fisher ex taq buffer
    Ex Taq Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore ex taq buffer
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    TaKaRa ex taq buffer
    Transactivation of the BLV LTR by various tax clones from BLV-infected cattle. (A and B) BLV tax genes were amplified by PCR with EX <t>Taq</t> (A) or KOD-Plus (B) <t>DNA</t> polymerase from the genomic DNAs of BLV-infected animals and from a molecular clone pBLV-IF and then subcloned into pME18Neo. 293T cells were transfected with the reporter plasmid pGV-BLTR, the reference plasmid pRL-SV40, and individual Tax-expressing plasmids. At 60 h after transfection, cells were recovered and the activities of firefly and Renilla luciferases were measured in lysates. For each sample, the firefly luciferase activity (pGV-BLTR) was normalized by reference to Renilla luciferase activity (pRL-SV40). The extent of transactivation (fold) was calculated by dividing the transactivation activity of the mutant Tax protein by that of wild-type Tax protein. (C) Summary of the results shown in panels A and B. The tax mutant clones were divided into three groups as follows, with wild-type activity being taken as 1: high, > 3-fold transactivation; medium, 3- to 0.5-fold transactivation; low,
    Ex Taq Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 3523 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Transactivation of the BLV LTR by various tax clones from BLV-infected cattle. (A and B) BLV tax genes were amplified by PCR with EX <t>Taq</t> (A) or KOD-Plus (B) <t>DNA</t> polymerase from the genomic DNAs of BLV-infected animals and from a molecular clone pBLV-IF and then subcloned into pME18Neo. 293T cells were transfected with the reporter plasmid pGV-BLTR, the reference plasmid pRL-SV40, and individual Tax-expressing plasmids. At 60 h after transfection, cells were recovered and the activities of firefly and Renilla luciferases were measured in lysates. For each sample, the firefly luciferase activity (pGV-BLTR) was normalized by reference to Renilla luciferase activity (pRL-SV40). The extent of transactivation (fold) was calculated by dividing the transactivation activity of the mutant Tax protein by that of wild-type Tax protein. (C) Summary of the results shown in panels A and B. The tax mutant clones were divided into three groups as follows, with wild-type activity being taken as 1: high, > 3-fold transactivation; medium, 3- to 0.5-fold transactivation; low,
    10× Ex Taq Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa 10x ex taq buffer
    Transactivation of the BLV LTR by various tax clones from BLV-infected cattle. (A and B) BLV tax genes were amplified by PCR with EX <t>Taq</t> (A) or KOD-Plus (B) <t>DNA</t> polymerase from the genomic DNAs of BLV-infected animals and from a molecular clone pBLV-IF and then subcloned into pME18Neo. 293T cells were transfected with the reporter plasmid pGV-BLTR, the reference plasmid pRL-SV40, and individual Tax-expressing plasmids. At 60 h after transfection, cells were recovered and the activities of firefly and Renilla luciferases were measured in lysates. For each sample, the firefly luciferase activity (pGV-BLTR) was normalized by reference to Renilla luciferase activity (pRL-SV40). The extent of transactivation (fold) was calculated by dividing the transactivation activity of the mutant Tax protein by that of wild-type Tax protein. (C) Summary of the results shown in panels A and B. The tax mutant clones were divided into three groups as follows, with wild-type activity being taken as 1: high, > 3-fold transactivation; medium, 3- to 0.5-fold transactivation; low,
    10x Ex Taq Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 89/100, based on 211 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa 1x ex taq buffer
    Transactivation of the BLV LTR by various tax clones from BLV-infected cattle. (A and B) BLV tax genes were amplified by PCR with EX <t>Taq</t> (A) or KOD-Plus (B) <t>DNA</t> polymerase from the genomic DNAs of BLV-infected animals and from a molecular clone pBLV-IF and then subcloned into pME18Neo. 293T cells were transfected with the reporter plasmid pGV-BLTR, the reference plasmid pRL-SV40, and individual Tax-expressing plasmids. At 60 h after transfection, cells were recovered and the activities of firefly and Renilla luciferases were measured in lysates. For each sample, the firefly luciferase activity (pGV-BLTR) was normalized by reference to Renilla luciferase activity (pRL-SV40). The extent of transactivation (fold) was calculated by dividing the transactivation activity of the mutant Tax protein by that of wild-type Tax protein. (C) Summary of the results shown in panels A and B. The tax mutant clones were divided into three groups as follows, with wild-type activity being taken as 1: high, > 3-fold transactivation; medium, 3- to 0.5-fold transactivation; low,
    1x Ex Taq Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa 1×ex taq buffer
    Transactivation of the BLV LTR by various tax clones from BLV-infected cattle. (A and B) BLV tax genes were amplified by PCR with EX <t>Taq</t> (A) or KOD-Plus (B) <t>DNA</t> polymerase from the genomic DNAs of BLV-infected animals and from a molecular clone pBLV-IF and then subcloned into pME18Neo. 293T cells were transfected with the reporter plasmid pGV-BLTR, the reference plasmid pRL-SV40, and individual Tax-expressing plasmids. At 60 h after transfection, cells were recovered and the activities of firefly and Renilla luciferases were measured in lysates. For each sample, the firefly luciferase activity (pGV-BLTR) was normalized by reference to Renilla luciferase activity (pRL-SV40). The extent of transactivation (fold) was calculated by dividing the transactivation activity of the mutant Tax protein by that of wild-type Tax protein. (C) Summary of the results shown in panels A and B. The tax mutant clones were divided into three groups as follows, with wild-type activity being taken as 1: high, > 3-fold transactivation; medium, 3- to 0.5-fold transactivation; low,
    1×Ex Taq Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa l×ex taq buffer
    Transactivation of the BLV LTR by various tax clones from BLV-infected cattle. (A and B) BLV tax genes were amplified by PCR with EX <t>Taq</t> (A) or KOD-Plus (B) <t>DNA</t> polymerase from the genomic DNAs of BLV-infected animals and from a molecular clone pBLV-IF and then subcloned into pME18Neo. 293T cells were transfected with the reporter plasmid pGV-BLTR, the reference plasmid pRL-SV40, and individual Tax-expressing plasmids. At 60 h after transfection, cells were recovered and the activities of firefly and Renilla luciferases were measured in lysates. For each sample, the firefly luciferase activity (pGV-BLTR) was normalized by reference to Renilla luciferase activity (pRL-SV40). The extent of transactivation (fold) was calculated by dividing the transactivation activity of the mutant Tax protein by that of wild-type Tax protein. (C) Summary of the results shown in panels A and B. The tax mutant clones were divided into three groups as follows, with wild-type activity being taken as 1: high, > 3-fold transactivation; medium, 3- to 0.5-fold transactivation; low,
    L×Ex Taq Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa pcr ex taq buffer
    Transactivation of the BLV LTR by various tax clones from BLV-infected cattle. (A and B) BLV tax genes were amplified by PCR with EX <t>Taq</t> (A) or KOD-Plus (B) <t>DNA</t> polymerase from the genomic DNAs of BLV-infected animals and from a molecular clone pBLV-IF and then subcloned into pME18Neo. 293T cells were transfected with the reporter plasmid pGV-BLTR, the reference plasmid pRL-SV40, and individual Tax-expressing plasmids. At 60 h after transfection, cells were recovered and the activities of firefly and Renilla luciferases were measured in lysates. For each sample, the firefly luciferase activity (pGV-BLTR) was normalized by reference to Renilla luciferase activity (pRL-SV40). The extent of transactivation (fold) was calculated by dividing the transactivation activity of the mutant Tax protein by that of wild-type Tax protein. (C) Summary of the results shown in panels A and B. The tax mutant clones were divided into three groups as follows, with wild-type activity being taken as 1: high, > 3-fold transactivation; medium, 3- to 0.5-fold transactivation; low,
    Pcr Ex Taq Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 89/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Boehringer Mannheim ex taq buffer
    Transactivation of the BLV LTR by various tax clones from BLV-infected cattle. (A and B) BLV tax genes were amplified by PCR with EX <t>Taq</t> (A) or KOD-Plus (B) <t>DNA</t> polymerase from the genomic DNAs of BLV-infected animals and from a molecular clone pBLV-IF and then subcloned into pME18Neo. 293T cells were transfected with the reporter plasmid pGV-BLTR, the reference plasmid pRL-SV40, and individual Tax-expressing plasmids. At 60 h after transfection, cells were recovered and the activities of firefly and Renilla luciferases were measured in lysates. For each sample, the firefly luciferase activity (pGV-BLTR) was normalized by reference to Renilla luciferase activity (pRL-SV40). The extent of transactivation (fold) was calculated by dividing the transactivation activity of the mutant Tax protein by that of wild-type Tax protein. (C) Summary of the results shown in panels A and B. The tax mutant clones were divided into three groups as follows, with wild-type activity being taken as 1: high, > 3-fold transactivation; medium, 3- to 0.5-fold transactivation; low,
    Ex Taq Buffer, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa takara ex taq buffer
    Transactivation of the BLV LTR by various tax clones from BLV-infected cattle. (A and B) BLV tax genes were amplified by PCR with EX <t>Taq</t> (A) or KOD-Plus (B) <t>DNA</t> polymerase from the genomic DNAs of BLV-infected animals and from a molecular clone pBLV-IF and then subcloned into pME18Neo. 293T cells were transfected with the reporter plasmid pGV-BLTR, the reference plasmid pRL-SV40, and individual Tax-expressing plasmids. At 60 h after transfection, cells were recovered and the activities of firefly and Renilla luciferases were measured in lysates. For each sample, the firefly luciferase activity (pGV-BLTR) was normalized by reference to Renilla luciferase activity (pRL-SV40). The extent of transactivation (fold) was calculated by dividing the transactivation activity of the mutant Tax protein by that of wild-type Tax protein. (C) Summary of the results shown in panels A and B. The tax mutant clones were divided into three groups as follows, with wild-type activity being taken as 1: high, > 3-fold transactivation; medium, 3- to 0.5-fold transactivation; low,
    Takara Ex Taq Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa 10 ex taq buffer
    Transactivation of the BLV LTR by various tax clones from BLV-infected cattle. (A and B) BLV tax genes were amplified by PCR with EX <t>Taq</t> (A) or KOD-Plus (B) <t>DNA</t> polymerase from the genomic DNAs of BLV-infected animals and from a molecular clone pBLV-IF and then subcloned into pME18Neo. 293T cells were transfected with the reporter plasmid pGV-BLTR, the reference plasmid pRL-SV40, and individual Tax-expressing plasmids. At 60 h after transfection, cells were recovered and the activities of firefly and Renilla luciferases were measured in lysates. For each sample, the firefly luciferase activity (pGV-BLTR) was normalized by reference to Renilla luciferase activity (pRL-SV40). The extent of transactivation (fold) was calculated by dividing the transactivation activity of the mutant Tax protein by that of wild-type Tax protein. (C) Summary of the results shown in panels A and B. The tax mutant clones were divided into three groups as follows, with wild-type activity being taken as 1: high, > 3-fold transactivation; medium, 3- to 0.5-fold transactivation; low,
    10 Ex Taq Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Transactivation of the BLV LTR by various tax clones from BLV-infected cattle. (A and B) BLV tax genes were amplified by PCR with EX <t>Taq</t> (A) or KOD-Plus (B) <t>DNA</t> polymerase from the genomic DNAs of BLV-infected animals and from a molecular clone pBLV-IF and then subcloned into pME18Neo. 293T cells were transfected with the reporter plasmid pGV-BLTR, the reference plasmid pRL-SV40, and individual Tax-expressing plasmids. At 60 h after transfection, cells were recovered and the activities of firefly and Renilla luciferases were measured in lysates. For each sample, the firefly luciferase activity (pGV-BLTR) was normalized by reference to Renilla luciferase activity (pRL-SV40). The extent of transactivation (fold) was calculated by dividing the transactivation activity of the mutant Tax protein by that of wild-type Tax protein. (C) Summary of the results shown in panels A and B. The tax mutant clones were divided into three groups as follows, with wild-type activity being taken as 1: high, > 3-fold transactivation; medium, 3- to 0.5-fold transactivation; low,
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    TaKaRa reverse primer r1 10x ex taq buffer pcr buffer
    Transactivation of the BLV LTR by various tax clones from BLV-infected cattle. (A and B) BLV tax genes were amplified by PCR with EX <t>Taq</t> (A) or KOD-Plus (B) <t>DNA</t> polymerase from the genomic DNAs of BLV-infected animals and from a molecular clone pBLV-IF and then subcloned into pME18Neo. 293T cells were transfected with the reporter plasmid pGV-BLTR, the reference plasmid pRL-SV40, and individual Tax-expressing plasmids. At 60 h after transfection, cells were recovered and the activities of firefly and Renilla luciferases were measured in lysates. For each sample, the firefly luciferase activity (pGV-BLTR) was normalized by reference to Renilla luciferase activity (pRL-SV40). The extent of transactivation (fold) was calculated by dividing the transactivation activity of the mutant Tax protein by that of wild-type Tax protein. (C) Summary of the results shown in panels A and B. The tax mutant clones were divided into three groups as follows, with wild-type activity being taken as 1: high, > 3-fold transactivation; medium, 3- to 0.5-fold transactivation; low,
    Reverse Primer R1 10x Ex Taq Buffer Pcr Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa sybr premix ex taq buffer
    Transactivation of the BLV LTR by various tax clones from BLV-infected cattle. (A and B) BLV tax genes were amplified by PCR with EX <t>Taq</t> (A) or KOD-Plus (B) <t>DNA</t> polymerase from the genomic DNAs of BLV-infected animals and from a molecular clone pBLV-IF and then subcloned into pME18Neo. 293T cells were transfected with the reporter plasmid pGV-BLTR, the reference plasmid pRL-SV40, and individual Tax-expressing plasmids. At 60 h after transfection, cells were recovered and the activities of firefly and Renilla luciferases were measured in lysates. For each sample, the firefly luciferase activity (pGV-BLTR) was normalized by reference to Renilla luciferase activity (pRL-SV40). The extent of transactivation (fold) was calculated by dividing the transactivation activity of the mutant Tax protein by that of wild-type Tax protein. (C) Summary of the results shown in panels A and B. The tax mutant clones were divided into three groups as follows, with wild-type activity being taken as 1: high, > 3-fold transactivation; medium, 3- to 0.5-fold transactivation; low,
    Sybr Premix Ex Taq Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Transactivation of the BLV LTR by various tax clones from BLV-infected cattle. (A and B) BLV tax genes were amplified by PCR with EX Taq (A) or KOD-Plus (B) DNA polymerase from the genomic DNAs of BLV-infected animals and from a molecular clone pBLV-IF and then subcloned into pME18Neo. 293T cells were transfected with the reporter plasmid pGV-BLTR, the reference plasmid pRL-SV40, and individual Tax-expressing plasmids. At 60 h after transfection, cells were recovered and the activities of firefly and Renilla luciferases were measured in lysates. For each sample, the firefly luciferase activity (pGV-BLTR) was normalized by reference to Renilla luciferase activity (pRL-SV40). The extent of transactivation (fold) was calculated by dividing the transactivation activity of the mutant Tax protein by that of wild-type Tax protein. (C) Summary of the results shown in panels A and B. The tax mutant clones were divided into three groups as follows, with wild-type activity being taken as 1: high, > 3-fold transactivation; medium, 3- to 0.5-fold transactivation; low,

    Journal: Journal of Virology

    Article Title: The Region between Amino Acids 245 and 265 of the Bovine Leukemia Virus (BLV) Tax Protein Restricts Transactivation Not Only via the BLV Enhancer but Also via Other Retrovirus Enhancers

    doi:

    Figure Lengend Snippet: Transactivation of the BLV LTR by various tax clones from BLV-infected cattle. (A and B) BLV tax genes were amplified by PCR with EX Taq (A) or KOD-Plus (B) DNA polymerase from the genomic DNAs of BLV-infected animals and from a molecular clone pBLV-IF and then subcloned into pME18Neo. 293T cells were transfected with the reporter plasmid pGV-BLTR, the reference plasmid pRL-SV40, and individual Tax-expressing plasmids. At 60 h after transfection, cells were recovered and the activities of firefly and Renilla luciferases were measured in lysates. For each sample, the firefly luciferase activity (pGV-BLTR) was normalized by reference to Renilla luciferase activity (pRL-SV40). The extent of transactivation (fold) was calculated by dividing the transactivation activity of the mutant Tax protein by that of wild-type Tax protein. (C) Summary of the results shown in panels A and B. The tax mutant clones were divided into three groups as follows, with wild-type activity being taken as 1: high, > 3-fold transactivation; medium, 3- to 0.5-fold transactivation; low,

    Article Snippet: Approximately 10 to 100 ng of genomic or plasmid DNA was amplified in a 30-μl reaction mixture that contained 1× EX Taq buffer (Takara Shuzo, Ohtsu, Japan) or 1× KOD-Plus buffer containing 1 mM MgSO4 , (Toyobo, Kyoto, Japan), 0.25 mM each deoxynucleoside triphosphate, 0.33 μM each oligonucleotide primer, and 1 U of EX Taq (Takara Shuzo) or KOD-Plus™ (Toyobo) DNA polymerase.

    Techniques: Clone Assay, Infection, Amplification, Polymerase Chain Reaction, Transfection, Plasmid Preparation, Expressing, Luciferase, Activity Assay, Mutagenesis