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  • 85
    Biotium evagreen biotium assays
    Reverse transcriptase assays. A. Fluorogenic assay. RT activity was measured by detection of RNA:DNA heteroduplex by fluorescence of <t>EvaGreen</t> binding. Oligo dT primed poly A was incubated at 37°C and 65°C in the presence of indicated Pol enzymes in manufacturer recommended buffers and dTTP. Fluorescence measurements were obtained every 6 seconds for 10 minutes. The initial slopes from a plot of RFU vs. time in seconds were determined by linear least square regression from 30 to 150 seconds at 37°C and from 30 to 90 seconds at 65°C. Error bars are standard error of regression slope. B. RT primer extension assay. HEX-labeled dT 20 primed poly A was incubated 10 minutes at 37°C and then 10 minutes at 65°C in the presence of indicated Pol enzymes and dTTP in manufacturer recommended buffers. Primer extension products were resolved by 10% denaturing PAGE and imaged on a Molecular Imager FX (Bio-Rad). Left facing triangle indicates migration of unextended dT20 primer and asterisk indicates bromophenol blue dye front. C. RT MS2-specific primer extension. 5′-labeled primer was annealed to MS2 RNA and incubated 10 minutes at 37°C and then 30 minutes at 65°C in the presence of indicated Pol enzymes with dNTPS (N = A,C,G,T) in manufacturer recommended buffers. Primer extension products were resolved by 5% denaturing PAGE. Lane 1 No RNA+MMLV RT; Lane 2: MS2 RNA No RT; Lane 3 MS2 RNA+MMLV RT, Lane 3 MS2 RNA+3173 Pol. Molecular weight in bases indicted. Red Arrow: ∼650 base MMLV extension product. Blue Arrow: ∼715 base PyroScript extension product. Green arrow: Non-templated MMLV reaction product.
    Evagreen Biotium Assays, supplied by Biotium, used in various techniques. Bioz Stars score: 85/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 164 article reviews
    Price from $9.99 to $1999.99
    evagreen biotium assays - by Bioz Stars, 2020-07
    85/100 stars
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    94
    Biotium biotium evagreen dye
    Reverse transcriptase assays. A. Fluorogenic assay. RT activity was measured by detection of RNA:DNA heteroduplex by fluorescence of <t>EvaGreen</t> binding. Oligo dT primed poly A was incubated at 37°C and 65°C in the presence of indicated Pol enzymes in manufacturer recommended buffers and dTTP. Fluorescence measurements were obtained every 6 seconds for 10 minutes. The initial slopes from a plot of RFU vs. time in seconds were determined by linear least square regression from 30 to 150 seconds at 37°C and from 30 to 90 seconds at 65°C. Error bars are standard error of regression slope. B. RT primer extension assay. HEX-labeled dT 20 primed poly A was incubated 10 minutes at 37°C and then 10 minutes at 65°C in the presence of indicated Pol enzymes and dTTP in manufacturer recommended buffers. Primer extension products were resolved by 10% denaturing PAGE and imaged on a Molecular Imager FX (Bio-Rad). Left facing triangle indicates migration of unextended dT20 primer and asterisk indicates bromophenol blue dye front. C. RT MS2-specific primer extension. 5′-labeled primer was annealed to MS2 RNA and incubated 10 minutes at 37°C and then 30 minutes at 65°C in the presence of indicated Pol enzymes with dNTPS (N = A,C,G,T) in manufacturer recommended buffers. Primer extension products were resolved by 5% denaturing PAGE. Lane 1 No RNA+MMLV RT; Lane 2: MS2 RNA No RT; Lane 3 MS2 RNA+MMLV RT, Lane 3 MS2 RNA+3173 Pol. Molecular weight in bases indicted. Red Arrow: ∼650 base MMLV extension product. Blue Arrow: ∼715 base PyroScript extension product. Green arrow: Non-templated MMLV reaction product.
    Biotium Evagreen Dye, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 33 article reviews
    Price from $9.99 to $1999.99
    biotium evagreen dye - by Bioz Stars, 2020-07
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    90
    Biotium evagreen biotium fluorescent
    Reverse transcriptase assays. A. Fluorogenic assay. RT activity was measured by detection of RNA:DNA heteroduplex by fluorescence of <t>EvaGreen</t> binding. Oligo dT primed poly A was incubated at 37°C and 65°C in the presence of indicated Pol enzymes in manufacturer recommended buffers and dTTP. Fluorescence measurements were obtained every 6 seconds for 10 minutes. The initial slopes from a plot of RFU vs. time in seconds were determined by linear least square regression from 30 to 150 seconds at 37°C and from 30 to 90 seconds at 65°C. Error bars are standard error of regression slope. B. RT primer extension assay. HEX-labeled dT 20 primed poly A was incubated 10 minutes at 37°C and then 10 minutes at 65°C in the presence of indicated Pol enzymes and dTTP in manufacturer recommended buffers. Primer extension products were resolved by 10% denaturing PAGE and imaged on a Molecular Imager FX (Bio-Rad). Left facing triangle indicates migration of unextended dT20 primer and asterisk indicates bromophenol blue dye front. C. RT MS2-specific primer extension. 5′-labeled primer was annealed to MS2 RNA and incubated 10 minutes at 37°C and then 30 minutes at 65°C in the presence of indicated Pol enzymes with dNTPS (N = A,C,G,T) in manufacturer recommended buffers. Primer extension products were resolved by 5% denaturing PAGE. Lane 1 No RNA+MMLV RT; Lane 2: MS2 RNA No RT; Lane 3 MS2 RNA+MMLV RT, Lane 3 MS2 RNA+3173 Pol. Molecular weight in bases indicted. Red Arrow: ∼650 base MMLV extension product. Blue Arrow: ∼715 base PyroScript extension product. Green arrow: Non-templated MMLV reaction product.
    Evagreen Biotium Fluorescent, supplied by Biotium, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 3 article reviews
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    evagreen biotium fluorescent - by Bioz Stars, 2020-07
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    86
    Biotium evagreen biotium chemistry
    Reverse transcriptase assays. A. Fluorogenic assay. RT activity was measured by detection of RNA:DNA heteroduplex by fluorescence of <t>EvaGreen</t> binding. Oligo dT primed poly A was incubated at 37°C and 65°C in the presence of indicated Pol enzymes in manufacturer recommended buffers and dTTP. Fluorescence measurements were obtained every 6 seconds for 10 minutes. The initial slopes from a plot of RFU vs. time in seconds were determined by linear least square regression from 30 to 150 seconds at 37°C and from 30 to 90 seconds at 65°C. Error bars are standard error of regression slope. B. RT primer extension assay. HEX-labeled dT 20 primed poly A was incubated 10 minutes at 37°C and then 10 minutes at 65°C in the presence of indicated Pol enzymes and dTTP in manufacturer recommended buffers. Primer extension products were resolved by 10% denaturing PAGE and imaged on a Molecular Imager FX (Bio-Rad). Left facing triangle indicates migration of unextended dT20 primer and asterisk indicates bromophenol blue dye front. C. RT MS2-specific primer extension. 5′-labeled primer was annealed to MS2 RNA and incubated 10 minutes at 37°C and then 30 minutes at 65°C in the presence of indicated Pol enzymes with dNTPS (N = A,C,G,T) in manufacturer recommended buffers. Primer extension products were resolved by 5% denaturing PAGE. Lane 1 No RNA+MMLV RT; Lane 2: MS2 RNA No RT; Lane 3 MS2 RNA+MMLV RT, Lane 3 MS2 RNA+3173 Pol. Molecular weight in bases indicted. Red Arrow: ∼650 base MMLV extension product. Blue Arrow: ∼715 base PyroScript extension product. Green arrow: Non-templated MMLV reaction product.
    Evagreen Biotium Chemistry, supplied by Biotium, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 3 article reviews
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    evagreen biotium chemistry - by Bioz Stars, 2020-07
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    90
    Biotium evagreen biotium qpcr mastermix
    Reverse transcriptase assays. A. Fluorogenic assay. RT activity was measured by detection of RNA:DNA heteroduplex by fluorescence of <t>EvaGreen</t> binding. Oligo dT primed poly A was incubated at 37°C and 65°C in the presence of indicated Pol enzymes in manufacturer recommended buffers and dTTP. Fluorescence measurements were obtained every 6 seconds for 10 minutes. The initial slopes from a plot of RFU vs. time in seconds were determined by linear least square regression from 30 to 150 seconds at 37°C and from 30 to 90 seconds at 65°C. Error bars are standard error of regression slope. B. RT primer extension assay. HEX-labeled dT 20 primed poly A was incubated 10 minutes at 37°C and then 10 minutes at 65°C in the presence of indicated Pol enzymes and dTTP in manufacturer recommended buffers. Primer extension products were resolved by 10% denaturing PAGE and imaged on a Molecular Imager FX (Bio-Rad). Left facing triangle indicates migration of unextended dT20 primer and asterisk indicates bromophenol blue dye front. C. RT MS2-specific primer extension. 5′-labeled primer was annealed to MS2 RNA and incubated 10 minutes at 37°C and then 30 minutes at 65°C in the presence of indicated Pol enzymes with dNTPS (N = A,C,G,T) in manufacturer recommended buffers. Primer extension products were resolved by 5% denaturing PAGE. Lane 1 No RNA+MMLV RT; Lane 2: MS2 RNA No RT; Lane 3 MS2 RNA+MMLV RT, Lane 3 MS2 RNA+3173 Pol. Molecular weight in bases indicted. Red Arrow: ∼650 base MMLV extension product. Blue Arrow: ∼715 base PyroScript extension product. Green arrow: Non-templated MMLV reaction product.
    Evagreen Biotium Qpcr Mastermix, supplied by Biotium, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 10 article reviews
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    evagreen biotium qpcr mastermix - by Bioz Stars, 2020-07
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    85
    Biotium 0 4×evagreen
    Reverse transcriptase assays. A. Fluorogenic assay. RT activity was measured by detection of RNA:DNA heteroduplex by fluorescence of <t>EvaGreen</t> binding. Oligo dT primed poly A was incubated at 37°C and 65°C in the presence of indicated Pol enzymes in manufacturer recommended buffers and dTTP. Fluorescence measurements were obtained every 6 seconds for 10 minutes. The initial slopes from a plot of RFU vs. time in seconds were determined by linear least square regression from 30 to 150 seconds at 37°C and from 30 to 90 seconds at 65°C. Error bars are standard error of regression slope. B. RT primer extension assay. HEX-labeled dT 20 primed poly A was incubated 10 minutes at 37°C and then 10 minutes at 65°C in the presence of indicated Pol enzymes and dTTP in manufacturer recommended buffers. Primer extension products were resolved by 10% denaturing PAGE and imaged on a Molecular Imager FX (Bio-Rad). Left facing triangle indicates migration of unextended dT20 primer and asterisk indicates bromophenol blue dye front. C. RT MS2-specific primer extension. 5′-labeled primer was annealed to MS2 RNA and incubated 10 minutes at 37°C and then 30 minutes at 65°C in the presence of indicated Pol enzymes with dNTPS (N = A,C,G,T) in manufacturer recommended buffers. Primer extension products were resolved by 5% denaturing PAGE. Lane 1 No RNA+MMLV RT; Lane 2: MS2 RNA No RT; Lane 3 MS2 RNA+MMLV RT, Lane 3 MS2 RNA+3173 Pol. Molecular weight in bases indicted. Red Arrow: ∼650 base MMLV extension product. Blue Arrow: ∼715 base PyroScript extension product. Green arrow: Non-templated MMLV reaction product.
    0 4×Evagreen, supplied by Biotium, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    0 4×evagreen - by Bioz Stars, 2020-07
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    92
    Biotium of 20x evagreen
    Reverse transcriptase assays. A. Fluorogenic assay. RT activity was measured by detection of RNA:DNA heteroduplex by fluorescence of <t>EvaGreen</t> binding. Oligo dT primed poly A was incubated at 37°C and 65°C in the presence of indicated Pol enzymes in manufacturer recommended buffers and dTTP. Fluorescence measurements were obtained every 6 seconds for 10 minutes. The initial slopes from a plot of RFU vs. time in seconds were determined by linear least square regression from 30 to 150 seconds at 37°C and from 30 to 90 seconds at 65°C. Error bars are standard error of regression slope. B. RT primer extension assay. HEX-labeled dT 20 primed poly A was incubated 10 minutes at 37°C and then 10 minutes at 65°C in the presence of indicated Pol enzymes and dTTP in manufacturer recommended buffers. Primer extension products were resolved by 10% denaturing PAGE and imaged on a Molecular Imager FX (Bio-Rad). Left facing triangle indicates migration of unextended dT20 primer and asterisk indicates bromophenol blue dye front. C. RT MS2-specific primer extension. 5′-labeled primer was annealed to MS2 RNA and incubated 10 minutes at 37°C and then 30 minutes at 65°C in the presence of indicated Pol enzymes with dNTPS (N = A,C,G,T) in manufacturer recommended buffers. Primer extension products were resolved by 5% denaturing PAGE. Lane 1 No RNA+MMLV RT; Lane 2: MS2 RNA No RT; Lane 3 MS2 RNA+MMLV RT, Lane 3 MS2 RNA+3173 Pol. Molecular weight in bases indicted. Red Arrow: ∼650 base MMLV extension product. Blue Arrow: ∼715 base PyroScript extension product. Green arrow: Non-templated MMLV reaction product.
    Of 20x Evagreen, supplied by Biotium, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    of 20x evagreen - by Bioz Stars, 2020-07
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    88
    Biotium 1x evagreen
    Reverse transcriptase assays. A. Fluorogenic assay. RT activity was measured by detection of RNA:DNA heteroduplex by fluorescence of <t>EvaGreen</t> binding. Oligo dT primed poly A was incubated at 37°C and 65°C in the presence of indicated Pol enzymes in manufacturer recommended buffers and dTTP. Fluorescence measurements were obtained every 6 seconds for 10 minutes. The initial slopes from a plot of RFU vs. time in seconds were determined by linear least square regression from 30 to 150 seconds at 37°C and from 30 to 90 seconds at 65°C. Error bars are standard error of regression slope. B. RT primer extension assay. HEX-labeled dT 20 primed poly A was incubated 10 minutes at 37°C and then 10 minutes at 65°C in the presence of indicated Pol enzymes and dTTP in manufacturer recommended buffers. Primer extension products were resolved by 10% denaturing PAGE and imaged on a Molecular Imager FX (Bio-Rad). Left facing triangle indicates migration of unextended dT20 primer and asterisk indicates bromophenol blue dye front. C. RT MS2-specific primer extension. 5′-labeled primer was annealed to MS2 RNA and incubated 10 minutes at 37°C and then 30 minutes at 65°C in the presence of indicated Pol enzymes with dNTPS (N = A,C,G,T) in manufacturer recommended buffers. Primer extension products were resolved by 5% denaturing PAGE. Lane 1 No RNA+MMLV RT; Lane 2: MS2 RNA No RT; Lane 3 MS2 RNA+MMLV RT, Lane 3 MS2 RNA+3173 Pol. Molecular weight in bases indicted. Red Arrow: ∼650 base MMLV extension product. Blue Arrow: ∼715 base PyroScript extension product. Green arrow: Non-templated MMLV reaction product.
    1x Evagreen, supplied by Biotium, used in various techniques. Bioz Stars score: 88/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 51 article reviews
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    1x evagreen - by Bioz Stars, 2020-07
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    93
    Biotium b evagreen
    Reverse transcriptase assays. A. Fluorogenic assay. RT activity was measured by detection of RNA:DNA heteroduplex by fluorescence of <t>EvaGreen</t> binding. Oligo dT primed poly A was incubated at 37°C and 65°C in the presence of indicated Pol enzymes in manufacturer recommended buffers and dTTP. Fluorescence measurements were obtained every 6 seconds for 10 minutes. The initial slopes from a plot of RFU vs. time in seconds were determined by linear least square regression from 30 to 150 seconds at 37°C and from 30 to 90 seconds at 65°C. Error bars are standard error of regression slope. B. RT primer extension assay. HEX-labeled dT 20 primed poly A was incubated 10 minutes at 37°C and then 10 minutes at 65°C in the presence of indicated Pol enzymes and dTTP in manufacturer recommended buffers. Primer extension products were resolved by 10% denaturing PAGE and imaged on a Molecular Imager FX (Bio-Rad). Left facing triangle indicates migration of unextended dT20 primer and asterisk indicates bromophenol blue dye front. C. RT MS2-specific primer extension. 5′-labeled primer was annealed to MS2 RNA and incubated 10 minutes at 37°C and then 30 minutes at 65°C in the presence of indicated Pol enzymes with dNTPS (N = A,C,G,T) in manufacturer recommended buffers. Primer extension products were resolved by 5% denaturing PAGE. Lane 1 No RNA+MMLV RT; Lane 2: MS2 RNA No RT; Lane 3 MS2 RNA+MMLV RT, Lane 3 MS2 RNA+3173 Pol. Molecular weight in bases indicted. Red Arrow: ∼650 base MMLV extension product. Blue Arrow: ∼715 base PyroScript extension product. Green arrow: Non-templated MMLV reaction product.
    B Evagreen, supplied by Biotium, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    b evagreen - by Bioz Stars, 2020-07
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    85
    Biotium 20×evagreen
    Reverse transcriptase assays. A. Fluorogenic assay. RT activity was measured by detection of RNA:DNA heteroduplex by fluorescence of <t>EvaGreen</t> binding. Oligo dT primed poly A was incubated at 37°C and 65°C in the presence of indicated Pol enzymes in manufacturer recommended buffers and dTTP. Fluorescence measurements were obtained every 6 seconds for 10 minutes. The initial slopes from a plot of RFU vs. time in seconds were determined by linear least square regression from 30 to 150 seconds at 37°C and from 30 to 90 seconds at 65°C. Error bars are standard error of regression slope. B. RT primer extension assay. HEX-labeled dT 20 primed poly A was incubated 10 minutes at 37°C and then 10 minutes at 65°C in the presence of indicated Pol enzymes and dTTP in manufacturer recommended buffers. Primer extension products were resolved by 10% denaturing PAGE and imaged on a Molecular Imager FX (Bio-Rad). Left facing triangle indicates migration of unextended dT20 primer and asterisk indicates bromophenol blue dye front. C. RT MS2-specific primer extension. 5′-labeled primer was annealed to MS2 RNA and incubated 10 minutes at 37°C and then 30 minutes at 65°C in the presence of indicated Pol enzymes with dNTPS (N = A,C,G,T) in manufacturer recommended buffers. Primer extension products were resolved by 5% denaturing PAGE. Lane 1 No RNA+MMLV RT; Lane 2: MS2 RNA No RT; Lane 3 MS2 RNA+MMLV RT, Lane 3 MS2 RNA+3173 Pol. Molecular weight in bases indicted. Red Arrow: ∼650 base MMLV extension product. Blue Arrow: ∼715 base PyroScript extension product. Green arrow: Non-templated MMLV reaction product.
    20×Evagreen, supplied by Biotium, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 7 article reviews
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    20×evagreen - by Bioz Stars, 2020-07
    85/100 stars
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    91
    Biotium 1×evagreen
    Reverse transcriptase assays. A. Fluorogenic assay. RT activity was measured by detection of RNA:DNA heteroduplex by fluorescence of <t>EvaGreen</t> binding. Oligo dT primed poly A was incubated at 37°C and 65°C in the presence of indicated Pol enzymes in manufacturer recommended buffers and dTTP. Fluorescence measurements were obtained every 6 seconds for 10 minutes. The initial slopes from a plot of RFU vs. time in seconds were determined by linear least square regression from 30 to 150 seconds at 37°C and from 30 to 90 seconds at 65°C. Error bars are standard error of regression slope. B. RT primer extension assay. HEX-labeled dT 20 primed poly A was incubated 10 minutes at 37°C and then 10 minutes at 65°C in the presence of indicated Pol enzymes and dTTP in manufacturer recommended buffers. Primer extension products were resolved by 10% denaturing PAGE and imaged on a Molecular Imager FX (Bio-Rad). Left facing triangle indicates migration of unextended dT20 primer and asterisk indicates bromophenol blue dye front. C. RT MS2-specific primer extension. 5′-labeled primer was annealed to MS2 RNA and incubated 10 minutes at 37°C and then 30 minutes at 65°C in the presence of indicated Pol enzymes with dNTPS (N = A,C,G,T) in manufacturer recommended buffers. Primer extension products were resolved by 5% denaturing PAGE. Lane 1 No RNA+MMLV RT; Lane 2: MS2 RNA No RT; Lane 3 MS2 RNA+MMLV RT, Lane 3 MS2 RNA+3173 Pol. Molecular weight in bases indicted. Red Arrow: ∼650 base MMLV extension product. Blue Arrow: ∼715 base PyroScript extension product. Green arrow: Non-templated MMLV reaction product.
    1×Evagreen, supplied by Biotium, used in various techniques. Bioz Stars score: 91/100, based on 175 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1×evagreen - by Bioz Stars, 2020-07
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    85
    Biotium 2x evagreen dye
    Reverse transcriptase assays. A. Fluorogenic assay. RT activity was measured by detection of RNA:DNA heteroduplex by fluorescence of <t>EvaGreen</t> binding. Oligo dT primed poly A was incubated at 37°C and 65°C in the presence of indicated Pol enzymes in manufacturer recommended buffers and dTTP. Fluorescence measurements were obtained every 6 seconds for 10 minutes. The initial slopes from a plot of RFU vs. time in seconds were determined by linear least square regression from 30 to 150 seconds at 37°C and from 30 to 90 seconds at 65°C. Error bars are standard error of regression slope. B. RT primer extension assay. HEX-labeled dT 20 primed poly A was incubated 10 minutes at 37°C and then 10 minutes at 65°C in the presence of indicated Pol enzymes and dTTP in manufacturer recommended buffers. Primer extension products were resolved by 10% denaturing PAGE and imaged on a Molecular Imager FX (Bio-Rad). Left facing triangle indicates migration of unextended dT20 primer and asterisk indicates bromophenol blue dye front. C. RT MS2-specific primer extension. 5′-labeled primer was annealed to MS2 RNA and incubated 10 minutes at 37°C and then 30 minutes at 65°C in the presence of indicated Pol enzymes with dNTPS (N = A,C,G,T) in manufacturer recommended buffers. Primer extension products were resolved by 5% denaturing PAGE. Lane 1 No RNA+MMLV RT; Lane 2: MS2 RNA No RT; Lane 3 MS2 RNA+MMLV RT, Lane 3 MS2 RNA+3173 Pol. Molecular weight in bases indicted. Red Arrow: ∼650 base MMLV extension product. Blue Arrow: ∼715 base PyroScript extension product. Green arrow: Non-templated MMLV reaction product.
    2x Evagreen Dye, supplied by Biotium, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Biotium 0 5x evagreen dye
    Reverse transcriptase assays. A. Fluorogenic assay. RT activity was measured by detection of RNA:DNA heteroduplex by fluorescence of <t>EvaGreen</t> binding. Oligo dT primed poly A was incubated at 37°C and 65°C in the presence of indicated Pol enzymes in manufacturer recommended buffers and dTTP. Fluorescence measurements were obtained every 6 seconds for 10 minutes. The initial slopes from a plot of RFU vs. time in seconds were determined by linear least square regression from 30 to 150 seconds at 37°C and from 30 to 90 seconds at 65°C. Error bars are standard error of regression slope. B. RT primer extension assay. HEX-labeled dT 20 primed poly A was incubated 10 minutes at 37°C and then 10 minutes at 65°C in the presence of indicated Pol enzymes and dTTP in manufacturer recommended buffers. Primer extension products were resolved by 10% denaturing PAGE and imaged on a Molecular Imager FX (Bio-Rad). Left facing triangle indicates migration of unextended dT20 primer and asterisk indicates bromophenol blue dye front. C. RT MS2-specific primer extension. 5′-labeled primer was annealed to MS2 RNA and incubated 10 minutes at 37°C and then 30 minutes at 65°C in the presence of indicated Pol enzymes with dNTPS (N = A,C,G,T) in manufacturer recommended buffers. Primer extension products were resolved by 5% denaturing PAGE. Lane 1 No RNA+MMLV RT; Lane 2: MS2 RNA No RT; Lane 3 MS2 RNA+MMLV RT, Lane 3 MS2 RNA+3173 Pol. Molecular weight in bases indicted. Red Arrow: ∼650 base MMLV extension product. Blue Arrow: ∼715 base PyroScript extension product. Green arrow: Non-templated MMLV reaction product.
    0 5x Evagreen Dye, supplied by Biotium, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Biotium evagreen fluorescent dye
    Construction and design of TINY. ( a ) A photograph of the measurement unit separated from the temperature-regulation unit. ( b ) A cross section of the measurement unit. Printed circuit boards are shown in green in the cross section. The dashed blue line shows the idealized path that sensed light takes from the excitation LED to the photodiodes. ( c ) LEDs are placed on the bottom side of the top PCB. When the LED shines blue, <t>Evagreen</t> dye is measured; yellow, ROX dye (used for normalization); and red, turbidity. The transmission characteristics of the dual bandpass filter are simplified here for clarity. ( d ) Looking down into the TINY system with the solar absorption plate removed, the measurement unit can be seen in the center of the temperature-regulation unit. ( e ) A cross section of the temperature-regulation unit (measurement unit excluded from cross section). After heat collection, the outer aluminum cylinder is covered with insulation on the top and bottom to slow heat loss (only shown on the sides in this cross section). Top and bottom insulation is attached to the TINY outer enclosure.
    Evagreen Fluorescent Dye, supplied by Biotium, used in various techniques. Bioz Stars score: 88/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    evagreen fluorescent dye - by Bioz Stars, 2020-07
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    88
    Biotium fast plus evagreen mastermix
    Construction and design of TINY. ( a ) A photograph of the measurement unit separated from the temperature-regulation unit. ( b ) A cross section of the measurement unit. Printed circuit boards are shown in green in the cross section. The dashed blue line shows the idealized path that sensed light takes from the excitation LED to the photodiodes. ( c ) LEDs are placed on the bottom side of the top PCB. When the LED shines blue, <t>Evagreen</t> dye is measured; yellow, ROX dye (used for normalization); and red, turbidity. The transmission characteristics of the dual bandpass filter are simplified here for clarity. ( d ) Looking down into the TINY system with the solar absorption plate removed, the measurement unit can be seen in the center of the temperature-regulation unit. ( e ) A cross section of the temperature-regulation unit (measurement unit excluded from cross section). After heat collection, the outer aluminum cylinder is covered with insulation on the top and bottom to slow heat loss (only shown on the sides in this cross section). Top and bottom insulation is attached to the TINY outer enclosure.
    Fast Plus Evagreen Mastermix, supplied by Biotium, used in various techniques. Bioz Stars score: 88/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    fast plus evagreen mastermix - by Bioz Stars, 2020-07
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    85
    Biotium evagreenr dye
    Construction and design of TINY. ( a ) A photograph of the measurement unit separated from the temperature-regulation unit. ( b ) A cross section of the measurement unit. Printed circuit boards are shown in green in the cross section. The dashed blue line shows the idealized path that sensed light takes from the excitation LED to the photodiodes. ( c ) LEDs are placed on the bottom side of the top PCB. When the LED shines blue, <t>Evagreen</t> dye is measured; yellow, ROX dye (used for normalization); and red, turbidity. The transmission characteristics of the dual bandpass filter are simplified here for clarity. ( d ) Looking down into the TINY system with the solar absorption plate removed, the measurement unit can be seen in the center of the temperature-regulation unit. ( e ) A cross section of the temperature-regulation unit (measurement unit excluded from cross section). After heat collection, the outer aluminum cylinder is covered with insulation on the top and bottom to slow heat loss (only shown on the sides in this cross section). Top and bottom insulation is attached to the TINY outer enclosure.
    Evagreenr Dye, supplied by Biotium, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biotium 1x fast evagreen
    Construction and design of TINY. ( a ) A photograph of the measurement unit separated from the temperature-regulation unit. ( b ) A cross section of the measurement unit. Printed circuit boards are shown in green in the cross section. The dashed blue line shows the idealized path that sensed light takes from the excitation LED to the photodiodes. ( c ) LEDs are placed on the bottom side of the top PCB. When the LED shines blue, <t>Evagreen</t> dye is measured; yellow, ROX dye (used for normalization); and red, turbidity. The transmission characteristics of the dual bandpass filter are simplified here for clarity. ( d ) Looking down into the TINY system with the solar absorption plate removed, the measurement unit can be seen in the center of the temperature-regulation unit. ( e ) A cross section of the temperature-regulation unit (measurement unit excluded from cross section). After heat collection, the outer aluminum cylinder is covered with insulation on the top and bottom to slow heat loss (only shown on the sides in this cross section). Top and bottom insulation is attached to the TINY outer enclosure.
    1x Fast Evagreen, supplied by Biotium, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biotium evagreen dye 20x
    Construction and design of TINY. ( a ) A photograph of the measurement unit separated from the temperature-regulation unit. ( b ) A cross section of the measurement unit. Printed circuit boards are shown in green in the cross section. The dashed blue line shows the idealized path that sensed light takes from the excitation LED to the photodiodes. ( c ) LEDs are placed on the bottom side of the top PCB. When the LED shines blue, <t>Evagreen</t> dye is measured; yellow, ROX dye (used for normalization); and red, turbidity. The transmission characteristics of the dual bandpass filter are simplified here for clarity. ( d ) Looking down into the TINY system with the solar absorption plate removed, the measurement unit can be seen in the center of the temperature-regulation unit. ( e ) A cross section of the temperature-regulation unit (measurement unit excluded from cross section). After heat collection, the outer aluminum cylinder is covered with insulation on the top and bottom to slow heat loss (only shown on the sides in this cross section). Top and bottom insulation is attached to the TINY outer enclosure.
    Evagreen Dye 20x, supplied by Biotium, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Biotium evagreen tm
    Construction and design of TINY. ( a ) A photograph of the measurement unit separated from the temperature-regulation unit. ( b ) A cross section of the measurement unit. Printed circuit boards are shown in green in the cross section. The dashed blue line shows the idealized path that sensed light takes from the excitation LED to the photodiodes. ( c ) LEDs are placed on the bottom side of the top PCB. When the LED shines blue, <t>Evagreen</t> dye is measured; yellow, ROX dye (used for normalization); and red, turbidity. The transmission characteristics of the dual bandpass filter are simplified here for clarity. ( d ) Looking down into the TINY system with the solar absorption plate removed, the measurement unit can be seen in the center of the temperature-regulation unit. ( e ) A cross section of the temperature-regulation unit (measurement unit excluded from cross section). After heat collection, the outer aluminum cylinder is covered with insulation on the top and bottom to slow heat loss (only shown on the sides in this cross section). Top and bottom insulation is attached to the TINY outer enclosure.
    Evagreen Tm, supplied by Biotium, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Reverse transcriptase assays. A. Fluorogenic assay. RT activity was measured by detection of RNA:DNA heteroduplex by fluorescence of EvaGreen binding. Oligo dT primed poly A was incubated at 37°C and 65°C in the presence of indicated Pol enzymes in manufacturer recommended buffers and dTTP. Fluorescence measurements were obtained every 6 seconds for 10 minutes. The initial slopes from a plot of RFU vs. time in seconds were determined by linear least square regression from 30 to 150 seconds at 37°C and from 30 to 90 seconds at 65°C. Error bars are standard error of regression slope. B. RT primer extension assay. HEX-labeled dT 20 primed poly A was incubated 10 minutes at 37°C and then 10 minutes at 65°C in the presence of indicated Pol enzymes and dTTP in manufacturer recommended buffers. Primer extension products were resolved by 10% denaturing PAGE and imaged on a Molecular Imager FX (Bio-Rad). Left facing triangle indicates migration of unextended dT20 primer and asterisk indicates bromophenol blue dye front. C. RT MS2-specific primer extension. 5′-labeled primer was annealed to MS2 RNA and incubated 10 minutes at 37°C and then 30 minutes at 65°C in the presence of indicated Pol enzymes with dNTPS (N = A,C,G,T) in manufacturer recommended buffers. Primer extension products were resolved by 5% denaturing PAGE. Lane 1 No RNA+MMLV RT; Lane 2: MS2 RNA No RT; Lane 3 MS2 RNA+MMLV RT, Lane 3 MS2 RNA+3173 Pol. Molecular weight in bases indicted. Red Arrow: ∼650 base MMLV extension product. Blue Arrow: ∼715 base PyroScript extension product. Green arrow: Non-templated MMLV reaction product.

    Journal: PLoS ONE

    Article Title: Thermostable DNA Polymerase from a Viral Metagenome Is a Potent RT-PCR Enzyme

    doi: 10.1371/journal.pone.0038371

    Figure Lengend Snippet: Reverse transcriptase assays. A. Fluorogenic assay. RT activity was measured by detection of RNA:DNA heteroduplex by fluorescence of EvaGreen binding. Oligo dT primed poly A was incubated at 37°C and 65°C in the presence of indicated Pol enzymes in manufacturer recommended buffers and dTTP. Fluorescence measurements were obtained every 6 seconds for 10 minutes. The initial slopes from a plot of RFU vs. time in seconds were determined by linear least square regression from 30 to 150 seconds at 37°C and from 30 to 90 seconds at 65°C. Error bars are standard error of regression slope. B. RT primer extension assay. HEX-labeled dT 20 primed poly A was incubated 10 minutes at 37°C and then 10 minutes at 65°C in the presence of indicated Pol enzymes and dTTP in manufacturer recommended buffers. Primer extension products were resolved by 10% denaturing PAGE and imaged on a Molecular Imager FX (Bio-Rad). Left facing triangle indicates migration of unextended dT20 primer and asterisk indicates bromophenol blue dye front. C. RT MS2-specific primer extension. 5′-labeled primer was annealed to MS2 RNA and incubated 10 minutes at 37°C and then 30 minutes at 65°C in the presence of indicated Pol enzymes with dNTPS (N = A,C,G,T) in manufacturer recommended buffers. Primer extension products were resolved by 5% denaturing PAGE. Lane 1 No RNA+MMLV RT; Lane 2: MS2 RNA No RT; Lane 3 MS2 RNA+MMLV RT, Lane 3 MS2 RNA+3173 Pol. Molecular weight in bases indicted. Red Arrow: ∼650 base MMLV extension product. Blue Arrow: ∼715 base PyroScript extension product. Green arrow: Non-templated MMLV reaction product.

    Article Snippet: For the Roche and the Lucigen reagents, a fluorescent DNA-binding dye, EvaGreen (Biotium, Hayward, CA), was added at 0.5×.

    Techniques: Activity Assay, Fluorescence, Binding Assay, Incubation, Primer Extension Assay, Labeling, Polyacrylamide Gel Electrophoresis, Migration, Molecular Weight

    Concept of the Tm mapping method. ( A ) The strategy for the primer designs is shown. Nested PCR is performed using seven bacterial universal primer sets, and then the seven Tm values are obtained. ( B ) Mapping the seven Tm values on two dimensions leads to the identification of the unique bacterial species-specific shape. The average of all seven Tm values includes the measurement error among trials; however, the Tm mapping shape is not affected by this type of error. ( C ) Using an analytical instrument with a high degree of thermal accuracy among PCR tubes and Tm value analysis with EvaGreen dye in 36 samples of the same bacterial DNA in the same trial, the tube-to-tube variation is within ±0.1 °C. ( D ) In order to analyze the Tm mapping “shape”, we developed a method to measure the distance of each individual Tm value from the average value. Tm values above the average receive a “+” designation, while those below the average receive a “−” designation. The Tm mapping shape is identified by comparing the seven distances obtained from the unknown bacteria to those in the database. ( E ) In order to identify a bacterial isolate, the identification software program calculates the Difference Values using the indicated formula. The closer the Difference Value is to zero, the more similar the Tm mapping shape is to the shape of a given species of pathogenic bacteria in the database.

    Journal: Scientific Reports

    Article Title: Melting Temperature Mapping Method: A Novel Method for Rapid Identification of Unknown Pathogenic Microorganisms within Three Hours of Sample Collection

    doi: 10.1038/srep12543

    Figure Lengend Snippet: Concept of the Tm mapping method. ( A ) The strategy for the primer designs is shown. Nested PCR is performed using seven bacterial universal primer sets, and then the seven Tm values are obtained. ( B ) Mapping the seven Tm values on two dimensions leads to the identification of the unique bacterial species-specific shape. The average of all seven Tm values includes the measurement error among trials; however, the Tm mapping shape is not affected by this type of error. ( C ) Using an analytical instrument with a high degree of thermal accuracy among PCR tubes and Tm value analysis with EvaGreen dye in 36 samples of the same bacterial DNA in the same trial, the tube-to-tube variation is within ±0.1 °C. ( D ) In order to analyze the Tm mapping “shape”, we developed a method to measure the distance of each individual Tm value from the average value. Tm values above the average receive a “+” designation, while those below the average receive a “−” designation. The Tm mapping shape is identified by comparing the seven distances obtained from the unknown bacteria to those in the database. ( E ) In order to identify a bacterial isolate, the identification software program calculates the Difference Values using the indicated formula. The closer the Difference Value is to zero, the more similar the Tm mapping shape is to the shape of a given species of pathogenic bacteria in the database.

    Article Snippet: In order to minimize this variation, we decided to use an analytical instrument with a high degree of thermal accuracy among PCR tubes and performed the Tm value analysis with EvaGreen dye (Biotium, Inc.).

    Techniques: Nested PCR, Polymerase Chain Reaction, Software

    Effect of dNTPs on T m measurement of DNA duplexes by HRM. The buffer contains 1x EvaGreen, 10 mM phosphate buffer (pH 7.4), and 100 mM NaCl. In this assay, 0, 0.02, 0.2, or 2 mM dNTPs were used.

    Journal: Journal of Analytical Methods in Chemistry

    Article Title: Assessment for Melting Temperature Measurement of Nucleic Acid by HRM

    doi: 10.1155/2016/5318935

    Figure Lengend Snippet: Effect of dNTPs on T m measurement of DNA duplexes by HRM. The buffer contains 1x EvaGreen, 10 mM phosphate buffer (pH 7.4), and 100 mM NaCl. In this assay, 0, 0.02, 0.2, or 2 mM dNTPs were used.

    Article Snippet: Materials and Reagents DNA and RNA oligonucleotides (in Tables and ) were synthesized from Invitrogen (Shanghai, China); the fluorescent dye EvaGreen 20x was obtained from Biotium (Hayward, CA).

    Techniques:

    Effect of EvaGreen concentration on T m of DNA duplex by HRM. DNA duplexes GC3/15 (a) or GC12/15 (b) of 1 μ M were measured in the solution with 0.25x, 0.5x, 1x, 2x, or 5x EvaGreen, 10 mM phosphate (pH 7.4), and 100 mM NaCl. Comparisons were done using one-way ANOVA analysis; ∗ P

    Journal: Journal of Analytical Methods in Chemistry

    Article Title: Assessment for Melting Temperature Measurement of Nucleic Acid by HRM

    doi: 10.1155/2016/5318935

    Figure Lengend Snippet: Effect of EvaGreen concentration on T m of DNA duplex by HRM. DNA duplexes GC3/15 (a) or GC12/15 (b) of 1 μ M were measured in the solution with 0.25x, 0.5x, 1x, 2x, or 5x EvaGreen, 10 mM phosphate (pH 7.4), and 100 mM NaCl. Comparisons were done using one-way ANOVA analysis; ∗ P

    Article Snippet: Materials and Reagents DNA and RNA oligonucleotides (in Tables and ) were synthesized from Invitrogen (Shanghai, China); the fluorescent dye EvaGreen 20x was obtained from Biotium (Hayward, CA).

    Techniques: Concentration Assay

    Fluorescence melting curves (a) and differential curves (b) of 6–20 bp DNA duplexes. Each DNA duplex (1 μ M) was measured in a 10 μ L solution containing 1x EvaGreen, 10 mM phosphate (pH 7.4), and 100 mM NaCl.

    Journal: Journal of Analytical Methods in Chemistry

    Article Title: Assessment for Melting Temperature Measurement of Nucleic Acid by HRM

    doi: 10.1155/2016/5318935

    Figure Lengend Snippet: Fluorescence melting curves (a) and differential curves (b) of 6–20 bp DNA duplexes. Each DNA duplex (1 μ M) was measured in a 10 μ L solution containing 1x EvaGreen, 10 mM phosphate (pH 7.4), and 100 mM NaCl.

    Article Snippet: Materials and Reagents DNA and RNA oligonucleotides (in Tables and ) were synthesized from Invitrogen (Shanghai, China); the fluorescent dye EvaGreen 20x was obtained from Biotium (Hayward, CA).

    Techniques: Fluorescence

    Construction and design of TINY. ( a ) A photograph of the measurement unit separated from the temperature-regulation unit. ( b ) A cross section of the measurement unit. Printed circuit boards are shown in green in the cross section. The dashed blue line shows the idealized path that sensed light takes from the excitation LED to the photodiodes. ( c ) LEDs are placed on the bottom side of the top PCB. When the LED shines blue, Evagreen dye is measured; yellow, ROX dye (used for normalization); and red, turbidity. The transmission characteristics of the dual bandpass filter are simplified here for clarity. ( d ) Looking down into the TINY system with the solar absorption plate removed, the measurement unit can be seen in the center of the temperature-regulation unit. ( e ) A cross section of the temperature-regulation unit (measurement unit excluded from cross section). After heat collection, the outer aluminum cylinder is covered with insulation on the top and bottom to slow heat loss (only shown on the sides in this cross section). Top and bottom insulation is attached to the TINY outer enclosure.

    Journal: Nature biomedical engineering

    Article Title: A portable device for nucleic acid quantification powered by sunlight, a flame or electricity

    doi: 10.1038/s41551-018-0286-y

    Figure Lengend Snippet: Construction and design of TINY. ( a ) A photograph of the measurement unit separated from the temperature-regulation unit. ( b ) A cross section of the measurement unit. Printed circuit boards are shown in green in the cross section. The dashed blue line shows the idealized path that sensed light takes from the excitation LED to the photodiodes. ( c ) LEDs are placed on the bottom side of the top PCB. When the LED shines blue, Evagreen dye is measured; yellow, ROX dye (used for normalization); and red, turbidity. The transmission characteristics of the dual bandpass filter are simplified here for clarity. ( d ) Looking down into the TINY system with the solar absorption plate removed, the measurement unit can be seen in the center of the temperature-regulation unit. ( e ) A cross section of the temperature-regulation unit (measurement unit excluded from cross section). After heat collection, the outer aluminum cylinder is covered with insulation on the top and bottom to slow heat loss (only shown on the sides in this cross section). Top and bottom insulation is attached to the TINY outer enclosure.

    Article Snippet: We also added Evagreen fluorescent dye (Biotium) to final concentration 1X, and ROX reference dye (Themo Fisher Scientific) to final concentration 2X.

    Techniques: Temperature Regulation, Transmission Assay