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  • 99
    Millipore ethylenediaminetetraacetic edta
    Transport of catechin, EGCG, and their niosomes by human intestinal Caco-2 cells. Notes: Schematic diagram of transcellular transport and different transporters in Caco-2 cells ( A ); effect of concentration on the flux of catechin, EGCG, and their niosomes (0, 10, 20, 50, and 100 μg/mL) through Caco-2 monolayer from apical to basolateral chamber ( B ); effect of temperature (4°C and 37°C), ATP inhibitor (sodium azide), P-gp inhibitor (verapamil), MRP2 inhibitor (MK-571) and absorption enhancer <t>(EDTA)</t> on transport of 100 μg/mL different drugs after 6 hours ( C ). Data are presented as mean ± SD (n=3). Abbreviations: EDTA, <t>ethylenediaminetetraacetic</t> acid; EGCG, (−)-epigallocatechin gallate; MRP2, multidrug resistance-associated protein 2; P-gp, permeability glycoprotein; SD, standard deviation.
    Ethylenediaminetetraacetic Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 263 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad ethylenediaminetetraacetic acid edta
    Effect of urea on the intrinsic fluorescence spectra of HsTIM (A and B). Isolation of the two proteins components of HsTIM by size exclusion chromatography (C). In A, the fluorescence spectrum of HsTIM, prepared as described under Material and Methods , was incubated at a concentration of 40 µg/ml in 100 mM triethanolamine, 10 mM <t>EDTA,</t> and 1 mM <t>dithiothreitol</t> (pH 7.4) for 60 min at 25°C with, and without, 6 M urea. At that time the spectra were recorded at an excitation wavelength of 280 nm. B shows the effect of different concentrations of urea on the λmax of its intrinsic fluorescence. C shows the size exclusion chromatography profile (see Methods section) of HsTIM incubated and eluted in a buffer containing 3 M urea. Note that two clearly distinguishable protein peaks were detected; the protein that eluted first was termed P1 and the second P2.
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    Promega ethylenediaminetetraacetic acid edta
    Effect of urea on the intrinsic fluorescence spectra of HsTIM (A and B). Isolation of the two proteins components of HsTIM by size exclusion chromatography (C). In A, the fluorescence spectrum of HsTIM, prepared as described under Material and Methods , was incubated at a concentration of 40 µg/ml in 100 mM triethanolamine, 10 mM <t>EDTA,</t> and 1 mM <t>dithiothreitol</t> (pH 7.4) for 60 min at 25°C with, and without, 6 M urea. At that time the spectra were recorded at an excitation wavelength of 280 nm. B shows the effect of different concentrations of urea on the λmax of its intrinsic fluorescence. C shows the size exclusion chromatography profile (see Methods section) of HsTIM incubated and eluted in a buffer containing 3 M urea. Note that two clearly distinguishable protein peaks were detected; the protein that eluted first was termed P1 and the second P2.
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    Kanto Chemical ethylenediaminetetraacetic acid edta
    Effect of urea on the intrinsic fluorescence spectra of HsTIM (A and B). Isolation of the two proteins components of HsTIM by size exclusion chromatography (C). In A, the fluorescence spectrum of HsTIM, prepared as described under Material and Methods , was incubated at a concentration of 40 µg/ml in 100 mM triethanolamine, 10 mM <t>EDTA,</t> and 1 mM <t>dithiothreitol</t> (pH 7.4) for 60 min at 25°C with, and without, 6 M urea. At that time the spectra were recorded at an excitation wavelength of 280 nm. B shows the effect of different concentrations of urea on the λmax of its intrinsic fluorescence. C shows the size exclusion chromatography profile (see Methods section) of HsTIM incubated and eluted in a buffer containing 3 M urea. Note that two clearly distinguishable protein peaks were detected; the protein that eluted first was termed P1 and the second P2.
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    Merck KGaA ethylenediaminetetraacetic acid edta
    Scanning electron micrographs (a) 1% oregano extract solution (OES) + 17% <t>ethylenediaminetetraacetic</t> acid <t>(EDTA)</t> (b) 2% OES + 17% EDTA (c) 5% OES + 17% EDTA
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    Merck & Co ethylenediaminetetraacetic acid edta
    Intracellular concentration of 1- N -phenylnaphthylamine in the presence of conessine (20 mg/L) and <t>ethylenediaminetetraacetic</t> acid <t>(EDTA)</t> (100 μM) in Pseudomonas aeruginosa K767 (PAO1) ( a ), P. aeruginosa K1455 (PAO1- nalB ) ( b ), and P. aeruginosa K1523 (PAO1-∆ mexB ) ( c ). Data were shown as average of two independent experiments. Error bars displayed ±SEM and * indicated significant ( P value ≤0.05)
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    Roche ethylenediaminetetraacetic acid edta
    Intracellular concentration of 1- N -phenylnaphthylamine in the presence of conessine (20 mg/L) and <t>ethylenediaminetetraacetic</t> acid <t>(EDTA)</t> (100 μM) in Pseudomonas aeruginosa K767 (PAO1) ( a ), P. aeruginosa K1455 (PAO1- nalB ) ( b ), and P. aeruginosa K1523 (PAO1-∆ mexB ) ( c ). Data were shown as average of two independent experiments. Error bars displayed ±SEM and * indicated significant ( P value ≤0.05)
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    Sinopharm ethylenediaminetetraacetic acid edta
    Intracellular concentration of 1- N -phenylnaphthylamine in the presence of conessine (20 mg/L) and <t>ethylenediaminetetraacetic</t> acid <t>(EDTA)</t> (100 μM) in Pseudomonas aeruginosa K767 (PAO1) ( a ), P. aeruginosa K1455 (PAO1- nalB ) ( b ), and P. aeruginosa K1523 (PAO1-∆ mexB ) ( c ). Data were shown as average of two independent experiments. Error bars displayed ±SEM and * indicated significant ( P value ≤0.05)
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    FUJIFILM ethylenediaminetetraacetic acid edta
    Intracellular concentration of 1- N -phenylnaphthylamine in the presence of conessine (20 mg/L) and <t>ethylenediaminetetraacetic</t> acid <t>(EDTA)</t> (100 μM) in Pseudomonas aeruginosa K767 (PAO1) ( a ), P. aeruginosa K1455 (PAO1- nalB ) ( b ), and P. aeruginosa K1523 (PAO1-∆ mexB ) ( c ). Data were shown as average of two independent experiments. Error bars displayed ±SEM and * indicated significant ( P value ≤0.05)
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    Dojindo Labs ethylenediaminetetraacetic acid edta
    Time courses of fluorescent intensity of bright spots corresponding to the binding and dissociation of single-molecule hybridization in (A) 8 mer–8 mer, (B) 12 mer–12 mer, and (C) dA 12 –dT 12 DNAs. Time-lapse images were obtained at an interval of 30 s. Experimental conditions: 200 mM NaCl <t>Tris-EDTA</t> (TE) buffer (10 mM Tris–HCl, pH 8.0, 1 mM <t>ethylenediaminetetraacetic</t> acid (EDTA), 200 mM NaCl) [Cy3-DNA] = 1 nM at 20 °C.
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    HiMedia Laboratories ethylenediaminetetraacetic acid edta
    Time courses of fluorescent intensity of bright spots corresponding to the binding and dissociation of single-molecule hybridization in (A) 8 mer–8 mer, (B) 12 mer–12 mer, and (C) dA 12 –dT 12 DNAs. Time-lapse images were obtained at an interval of 30 s. Experimental conditions: 200 mM NaCl <t>Tris-EDTA</t> (TE) buffer (10 mM Tris–HCl, pH 8.0, 1 mM <t>ethylenediaminetetraacetic</t> acid (EDTA), 200 mM NaCl) [Cy3-DNA] = 1 nM at 20 °C.
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    Becton Dickinson ethylenediaminetetraacetic acid edta
    Impact of different blood anticoagulants on the surface expression of mCD14 on bovine monocytes . Whole blood samples obtained from 18 Holstein cows were anticoagulated with: sodium heparin (HEPARIN), sodium citrate (CITRATE) and <t>ethylenediaminetetraacetic</t> acid <t>(EDTA).</t> The percentage of monocytes expressing mCD14 (2A) and the mean channel fluorescence intensity (MFI) (2B) was measured. Results for each treatment are the mean from 6 cows. Treatment means with different superscripts are significantly different using Scheffe's multiple comparison tests.
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    Boehringer Mannheim ethylenediaminetetraacetic acid edta
    Impact of different blood anticoagulants on the surface expression of mCD14 on bovine monocytes . Whole blood samples obtained from 18 Holstein cows were anticoagulated with: sodium heparin (HEPARIN), sodium citrate (CITRATE) and <t>ethylenediaminetetraacetic</t> acid <t>(EDTA).</t> The percentage of monocytes expressing mCD14 (2A) and the mean channel fluorescence intensity (MFI) (2B) was measured. Results for each treatment are the mean from 6 cows. Treatment means with different superscripts are significantly different using Scheffe's multiple comparison tests.
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    Pulpdent ethylenediaminetetraacetic acid edta
    Impact of different blood anticoagulants on the surface expression of mCD14 on bovine monocytes . Whole blood samples obtained from 18 Holstein cows were anticoagulated with: sodium heparin (HEPARIN), sodium citrate (CITRATE) and <t>ethylenediaminetetraacetic</t> acid <t>(EDTA).</t> The percentage of monocytes expressing mCD14 (2A) and the mean channel fluorescence intensity (MFI) (2B) was measured. Results for each treatment are the mean from 6 cows. Treatment means with different superscripts are significantly different using Scheffe's multiple comparison tests.
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    Corning Life Sciences ethylenediaminetetraacetic acid edta
    Impact of different blood anticoagulants on the surface expression of mCD14 on bovine monocytes . Whole blood samples obtained from 18 Holstein cows were anticoagulated with: sodium heparin (HEPARIN), sodium citrate (CITRATE) and <t>ethylenediaminetetraacetic</t> acid <t>(EDTA).</t> The percentage of monocytes expressing mCD14 (2A) and the mean channel fluorescence intensity (MFI) (2B) was measured. Results for each treatment are the mean from 6 cows. Treatment means with different superscripts are significantly different using Scheffe's multiple comparison tests.
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    Fisher Scientific ethylenediaminetetraacetic acid edta
    Impact of different blood anticoagulants on the surface expression of mCD14 on bovine monocytes . Whole blood samples obtained from 18 Holstein cows were anticoagulated with: sodium heparin (HEPARIN), sodium citrate (CITRATE) and <t>ethylenediaminetetraacetic</t> acid <t>(EDTA).</t> The percentage of monocytes expressing mCD14 (2A) and the mean channel fluorescence intensity (MFI) (2B) was measured. Results for each treatment are the mean from 6 cows. Treatment means with different superscripts are significantly different using Scheffe's multiple comparison tests.
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    Avantor ethylenediaminetetraacetic acid edta
    Impact of different blood anticoagulants on the surface expression of mCD14 on bovine monocytes . Whole blood samples obtained from 18 Holstein cows were anticoagulated with: sodium heparin (HEPARIN), sodium citrate (CITRATE) and <t>ethylenediaminetetraacetic</t> acid <t>(EDTA).</t> The percentage of monocytes expressing mCD14 (2A) and the mean channel fluorescence intensity (MFI) (2B) was measured. Results for each treatment are the mean from 6 cows. Treatment means with different superscripts are significantly different using Scheffe's multiple comparison tests.
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    Beyotime ethylenediaminetetraacetic acid edta
    Impact of different blood anticoagulants on the surface expression of mCD14 on bovine monocytes . Whole blood samples obtained from 18 Holstein cows were anticoagulated with: sodium heparin (HEPARIN), sodium citrate (CITRATE) and <t>ethylenediaminetetraacetic</t> acid <t>(EDTA).</t> The percentage of monocytes expressing mCD14 (2A) and the mean channel fluorescence intensity (MFI) (2B) was measured. Results for each treatment are the mean from 6 cows. Treatment means with different superscripts are significantly different using Scheffe's multiple comparison tests.
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    Amresco ethylenediaminetetraacetic acid edta
    Impact of different blood anticoagulants on the surface expression of mCD14 on bovine monocytes . Whole blood samples obtained from 18 Holstein cows were anticoagulated with: sodium heparin (HEPARIN), sodium citrate (CITRATE) and <t>ethylenediaminetetraacetic</t> acid <t>(EDTA).</t> The percentage of monocytes expressing mCD14 (2A) and the mean channel fluorescence intensity (MFI) (2B) was measured. Results for each treatment are the mean from 6 cows. Treatment means with different superscripts are significantly different using Scheffe's multiple comparison tests.
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    Nacalai ethylenediaminetetraacetic acid edta
    Impact of different blood anticoagulants on the surface expression of mCD14 on bovine monocytes . Whole blood samples obtained from 18 Holstein cows were anticoagulated with: sodium heparin (HEPARIN), sodium citrate (CITRATE) and <t>ethylenediaminetetraacetic</t> acid <t>(EDTA).</t> The percentage of monocytes expressing mCD14 (2A) and the mean channel fluorescence intensity (MFI) (2B) was measured. Results for each treatment are the mean from 6 cows. Treatment means with different superscripts are significantly different using Scheffe's multiple comparison tests.
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    Nanjing Chemical Reagent Co Ltd ethylenediaminetetraacetic acid edta
    Impact of different blood anticoagulants on the surface expression of mCD14 on bovine monocytes . Whole blood samples obtained from 18 Holstein cows were anticoagulated with: sodium heparin (HEPARIN), sodium citrate (CITRATE) and <t>ethylenediaminetetraacetic</t> acid <t>(EDTA).</t> The percentage of monocytes expressing mCD14 (2A) and the mean channel fluorescence intensity (MFI) (2B) was measured. Results for each treatment are the mean from 6 cows. Treatment means with different superscripts are significantly different using Scheffe's multiple comparison tests.
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    Impact of different blood anticoagulants on the surface expression of mCD14 on bovine monocytes . Whole blood samples obtained from 18 Holstein cows were anticoagulated with: sodium heparin (HEPARIN), sodium citrate (CITRATE) and <t>ethylenediaminetetraacetic</t> acid <t>(EDTA).</t> The percentage of monocytes expressing mCD14 (2A) and the mean channel fluorescence intensity (MFI) (2B) was measured. Results for each treatment are the mean from 6 cows. Treatment means with different superscripts are significantly different using Scheffe's multiple comparison tests.
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    Impact of different blood anticoagulants on the surface expression of mCD14 on bovine monocytes . Whole blood samples obtained from 18 Holstein cows were anticoagulated with: sodium heparin (HEPARIN), sodium citrate (CITRATE) and <t>ethylenediaminetetraacetic</t> acid <t>(EDTA).</t> The percentage of monocytes expressing mCD14 (2A) and the mean channel fluorescence intensity (MFI) (2B) was measured. Results for each treatment are the mean from 6 cows. Treatment means with different superscripts are significantly different using Scheffe's multiple comparison tests.
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    Image Search Results


    Transport of catechin, EGCG, and their niosomes by human intestinal Caco-2 cells. Notes: Schematic diagram of transcellular transport and different transporters in Caco-2 cells ( A ); effect of concentration on the flux of catechin, EGCG, and their niosomes (0, 10, 20, 50, and 100 μg/mL) through Caco-2 monolayer from apical to basolateral chamber ( B ); effect of temperature (4°C and 37°C), ATP inhibitor (sodium azide), P-gp inhibitor (verapamil), MRP2 inhibitor (MK-571) and absorption enhancer (EDTA) on transport of 100 μg/mL different drugs after 6 hours ( C ). Data are presented as mean ± SD (n=3). Abbreviations: EDTA, ethylenediaminetetraacetic acid; EGCG, (−)-epigallocatechin gallate; MRP2, multidrug resistance-associated protein 2; P-gp, permeability glycoprotein; SD, standard deviation.

    Journal: International Journal of Nanomedicine

    Article Title: Enhanced uptake and transport of (+)-catechin and (-)-epigallocatechin gallate in niosomal formulation by human intestinal Caco-2 cells

    doi: 10.2147/IJN.S59331

    Figure Lengend Snippet: Transport of catechin, EGCG, and their niosomes by human intestinal Caco-2 cells. Notes: Schematic diagram of transcellular transport and different transporters in Caco-2 cells ( A ); effect of concentration on the flux of catechin, EGCG, and their niosomes (0, 10, 20, 50, and 100 μg/mL) through Caco-2 monolayer from apical to basolateral chamber ( B ); effect of temperature (4°C and 37°C), ATP inhibitor (sodium azide), P-gp inhibitor (verapamil), MRP2 inhibitor (MK-571) and absorption enhancer (EDTA) on transport of 100 μg/mL different drugs after 6 hours ( C ). Data are presented as mean ± SD (n=3). Abbreviations: EDTA, ethylenediaminetetraacetic acid; EGCG, (−)-epigallocatechin gallate; MRP2, multidrug resistance-associated protein 2; P-gp, permeability glycoprotein; SD, standard deviation.

    Article Snippet: Chemicals and reagents Catechin, EGCG, sorbitan monostearate (Span 60), CH, dihexadecyl phosphate, fluorescein isothiocyanate (FITC), sulforhodamine B (SRB), fluorescein sodium salt, ascorbic acid (AA), sodium azide, verapamil, 5-(3-(2-(7-chloroquinolin-2-yl)ethenyl)phenyl)-8-dimethylcarbamyl-4,6-dithiaoctanoic acid sodium salt hydrate (MK-571), and ethylenediaminetetraacetic acid (EDTA) were purchased from Sigma-Aldrich (St Louis, MO, USA).

    Techniques: Concentration Assay, Permeability, Standard Deviation

    Effect of urea on the intrinsic fluorescence spectra of HsTIM (A and B). Isolation of the two proteins components of HsTIM by size exclusion chromatography (C). In A, the fluorescence spectrum of HsTIM, prepared as described under Material and Methods , was incubated at a concentration of 40 µg/ml in 100 mM triethanolamine, 10 mM EDTA, and 1 mM dithiothreitol (pH 7.4) for 60 min at 25°C with, and without, 6 M urea. At that time the spectra were recorded at an excitation wavelength of 280 nm. B shows the effect of different concentrations of urea on the λmax of its intrinsic fluorescence. C shows the size exclusion chromatography profile (see Methods section) of HsTIM incubated and eluted in a buffer containing 3 M urea. Note that two clearly distinguishable protein peaks were detected; the protein that eluted first was termed P1 and the second P2.

    Journal: PLoS ONE

    Article Title: A Ribosomal Misincorporation of Lys for Arg in Human Triosephosphate Isomerase Expressed in Escherichia coli Gives Rise to Two Protein Populations

    doi: 10.1371/journal.pone.0021035

    Figure Lengend Snippet: Effect of urea on the intrinsic fluorescence spectra of HsTIM (A and B). Isolation of the two proteins components of HsTIM by size exclusion chromatography (C). In A, the fluorescence spectrum of HsTIM, prepared as described under Material and Methods , was incubated at a concentration of 40 µg/ml in 100 mM triethanolamine, 10 mM EDTA, and 1 mM dithiothreitol (pH 7.4) for 60 min at 25°C with, and without, 6 M urea. At that time the spectra were recorded at an excitation wavelength of 280 nm. B shows the effect of different concentrations of urea on the λmax of its intrinsic fluorescence. C shows the size exclusion chromatography profile (see Methods section) of HsTIM incubated and eluted in a buffer containing 3 M urea. Note that two clearly distinguishable protein peaks were detected; the protein that eluted first was termed P1 and the second P2.

    Article Snippet: Generally 300 µl of 20 mM triethanolamine, 0.2 mM EDTA, 200 mM NaCl and 1 mM dithiothreitol (pH 7.4) that contained between 25 to 500 µg of protein were applied and eluted with the same buffer. the columns were calibrated with a gel filtration standard (Bio-Rad) containing the following globular protein markers (molecular mass and retention volumes are reported): thyroglobulin (bovine) (669 kDa, 9.8 ml), γ-globulin (bovine) (158 kDa, 12.9 ml), ovalbumin (chicken) (43 kDa, 15.8 ml), myoglobin (horse) (17 kDa, 17.7 ml), and vitamin B12 (1.35 kDa, 21.1 ml).

    Techniques: Fluorescence, Isolation, Size-exclusion Chromatography, Incubation, Concentration Assay

    Scanning electron micrographs (a) 1% oregano extract solution (OES) + 17% ethylenediaminetetraacetic acid (EDTA) (b) 2% OES + 17% EDTA (c) 5% OES + 17% EDTA

    Journal: European Journal of Dentistry

    Article Title: Antibacterial and smear layer removal capability of oregano extract solution

    doi: 10.4103/1305-7456.149633

    Figure Lengend Snippet: Scanning electron micrographs (a) 1% oregano extract solution (OES) + 17% ethylenediaminetetraacetic acid (EDTA) (b) 2% OES + 17% EDTA (c) 5% OES + 17% EDTA

    Article Snippet: The smear layer was removed in the first seven groups with 3 ml 17% ethylenediaminetetraacetic acid (EDTA) (Merck KGaA, Darmstadt, Germany) for 1-min, followed by 3 ml 5.25% NaOCl (Wizard, Ankara, Turkey) for 1-min, and then 5 ml distillate water for 1-min as outlined by Teixeira et al .

    Techniques:

    Scanning electron micrographs of 5.25 NaOCl + 17% ethylenediaminetetraacetic acid

    Journal: European Journal of Dentistry

    Article Title: Antibacterial and smear layer removal capability of oregano extract solution

    doi: 10.4103/1305-7456.149633

    Figure Lengend Snippet: Scanning electron micrographs of 5.25 NaOCl + 17% ethylenediaminetetraacetic acid

    Article Snippet: The smear layer was removed in the first seven groups with 3 ml 17% ethylenediaminetetraacetic acid (EDTA) (Merck KGaA, Darmstadt, Germany) for 1-min, followed by 3 ml 5.25% NaOCl (Wizard, Ankara, Turkey) for 1-min, and then 5 ml distillate water for 1-min as outlined by Teixeira et al .

    Techniques:

    Intracellular concentration of 1- N -phenylnaphthylamine in the presence of conessine (20 mg/L) and ethylenediaminetetraacetic acid (EDTA) (100 μM) in Pseudomonas aeruginosa K767 (PAO1) ( a ), P. aeruginosa K1455 (PAO1- nalB ) ( b ), and P. aeruginosa K1523 (PAO1-∆ mexB ) ( c ). Data were shown as average of two independent experiments. Error bars displayed ±SEM and * indicated significant ( P value ≤0.05)

    Journal: BMC Complementary and Alternative Medicine

    Article Title: Conessine as a novel inhibitor of multidrug efflux pump systems in Pseudomonas aeruginosa

    doi: 10.1186/s12906-017-1913-y

    Figure Lengend Snippet: Intracellular concentration of 1- N -phenylnaphthylamine in the presence of conessine (20 mg/L) and ethylenediaminetetraacetic acid (EDTA) (100 μM) in Pseudomonas aeruginosa K767 (PAO1) ( a ), P. aeruginosa K1455 (PAO1- nalB ) ( b ), and P. aeruginosa K1523 (PAO1-∆ mexB ) ( c ). Data were shown as average of two independent experiments. Error bars displayed ±SEM and * indicated significant ( P value ≤0.05)

    Article Snippet: Dimethylsulfoxide (DMSO) and ethylenediaminetetraacetic acid (EDTA) were obtained from Merck (Merck, Germany).

    Techniques: Concentration Assay

    Time courses of fluorescent intensity of bright spots corresponding to the binding and dissociation of single-molecule hybridization in (A) 8 mer–8 mer, (B) 12 mer–12 mer, and (C) dA 12 –dT 12 DNAs. Time-lapse images were obtained at an interval of 30 s. Experimental conditions: 200 mM NaCl Tris-EDTA (TE) buffer (10 mM Tris–HCl, pH 8.0, 1 mM ethylenediaminetetraacetic acid (EDTA), 200 mM NaCl) [Cy3-DNA] = 1 nM at 20 °C.

    Journal: ACS Omega

    Article Title: Probing Multiple Binding Modes of DNA Hybridization: A Comparison between Single-Molecule Observations and Ensemble Measurements

    doi: 10.1021/acsomega.8b00135

    Figure Lengend Snippet: Time courses of fluorescent intensity of bright spots corresponding to the binding and dissociation of single-molecule hybridization in (A) 8 mer–8 mer, (B) 12 mer–12 mer, and (C) dA 12 –dT 12 DNAs. Time-lapse images were obtained at an interval of 30 s. Experimental conditions: 200 mM NaCl Tris-EDTA (TE) buffer (10 mM Tris–HCl, pH 8.0, 1 mM ethylenediaminetetraacetic acid (EDTA), 200 mM NaCl) [Cy3-DNA] = 1 nM at 20 °C.

    Article Snippet: 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and ethylenediaminetetraacetic acid (EDTA) were purchased from Dojindo (Kumamoto, Japan).

    Techniques: Binding Assay, Hybridization

    Impact of different blood anticoagulants on the surface expression of mCD14 on bovine monocytes . Whole blood samples obtained from 18 Holstein cows were anticoagulated with: sodium heparin (HEPARIN), sodium citrate (CITRATE) and ethylenediaminetetraacetic acid (EDTA). The percentage of monocytes expressing mCD14 (2A) and the mean channel fluorescence intensity (MFI) (2B) was measured. Results for each treatment are the mean from 6 cows. Treatment means with different superscripts are significantly different using Scheffe's multiple comparison tests.

    Journal: BMC Research Notes

    Article Title: The influence of different anticoagulants and sample preparation methods on measurement of mCD14 on bovine monocytes and polymorphonuclear neutrophil leukocytes

    doi: 10.1186/1756-0500-5-93

    Figure Lengend Snippet: Impact of different blood anticoagulants on the surface expression of mCD14 on bovine monocytes . Whole blood samples obtained from 18 Holstein cows were anticoagulated with: sodium heparin (HEPARIN), sodium citrate (CITRATE) and ethylenediaminetetraacetic acid (EDTA). The percentage of monocytes expressing mCD14 (2A) and the mean channel fluorescence intensity (MFI) (2B) was measured. Results for each treatment are the mean from 6 cows. Treatment means with different superscripts are significantly different using Scheffe's multiple comparison tests.

    Article Snippet: Blood sampling Blood was collected aseptically from the caudal vein by venipuncture into one of three vacutainer tubes containing either sodium heparin (HEPARIN), sodium citrate (CITRATE) or ethylenediaminetetraacetic acid (EDTA) (Becton Dickinson, Franklin Lakes, NJ, USA).

    Techniques: Expressing, Fluorescence

    Impact of different blood anticoagulants on the surface expression of CD14 on bovine neutrophils . Whole blood samples obtained from 18 Holstein cows were anticoagulated with: sodium heparin (HEPARIN), sodium citrate (CITRATE) and ethylenediaminetetraacetic acid (EDTA). The percentage of PMN expressing mCD14 (1A) and the mean channel fluorescence intensity (MFI) (1B) was measured. Results for each treatment are the mean from 6 cows. Treatment means with different superscripts are significantly different ( p

    Journal: BMC Research Notes

    Article Title: The influence of different anticoagulants and sample preparation methods on measurement of mCD14 on bovine monocytes and polymorphonuclear neutrophil leukocytes

    doi: 10.1186/1756-0500-5-93

    Figure Lengend Snippet: Impact of different blood anticoagulants on the surface expression of CD14 on bovine neutrophils . Whole blood samples obtained from 18 Holstein cows were anticoagulated with: sodium heparin (HEPARIN), sodium citrate (CITRATE) and ethylenediaminetetraacetic acid (EDTA). The percentage of PMN expressing mCD14 (1A) and the mean channel fluorescence intensity (MFI) (1B) was measured. Results for each treatment are the mean from 6 cows. Treatment means with different superscripts are significantly different ( p

    Article Snippet: Blood sampling Blood was collected aseptically from the caudal vein by venipuncture into one of three vacutainer tubes containing either sodium heparin (HEPARIN), sodium citrate (CITRATE) or ethylenediaminetetraacetic acid (EDTA) (Becton Dickinson, Franklin Lakes, NJ, USA).

    Techniques: Expressing, Fluorescence