ethylenediaminetetraacetic acid Search Results


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  • 99
    Millipore ethylenediaminetetra­acetic acid
    Transport of catechin, EGCG, and their niosomes by human intestinal Caco-2 cells. Notes: Schematic diagram of transcellular transport and different transporters in Caco-2 cells ( A ); effect of concentration on the flux of catechin, EGCG, and their niosomes (0, 10, 20, 50, and 100 μg/mL) through Caco-2 monolayer from apical to basolateral chamber ( B ); effect of temperature (4°C and 37°C), ATP inhibitor (sodium azide), P-gp inhibitor (verapamil), MRP2 inhibitor (MK-571) and absorption enhancer <t>(EDTA)</t> on transport of 100 μg/mL different drugs after 6 hours ( C ). Data are presented as mean ± SD (n=3). Abbreviations: EDTA, <t>ethylenediaminetetraacetic</t> acid; EGCG, (−)-epigallocatechin gallate; MRP2, multidrug resistance-associated protein 2; P-gp, permeability glycoprotein; SD, standard deviation.
    Ethylenediaminetetra­Acetic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 273 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore edta
    Laminin HBD peptides promote cell adhesion in vitro. a , b A total of 3000 cells/well human lung fibroblasts were cultured a without or b with 5 mM <t>EDTA</t> in FGM-2 culture media containing 1% <t>FBS.</t> c , d A total of 3000 cells/well HUVEC were cultured c without or d with 5 mM EDTA in EBM-2 culture media containing 100 ng/mL VEGF-A165 and 1% FBS. Cells were plated on 1 μg/mL laminin peptide pre-coated non-tissue culture-treated plates and incubated for 30 min at 37 °C. After plate washes, cell numbers were quantified using a CyQUANT assay ( n = 10, mean ± SEM). The signals obtained from BSA-coated wells are normalized to 1, and relative fold increases of cell numbers were calculated. Statistical analyses were performed using ANOVA with Tukey’s test. Kruskal–Wallis test followed by Dunn’s multiple comparison was used in b , c . * p
    Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 32373 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Merck KGaA ethylenediaminetetraacetic acids
    Laminin HBD peptides promote cell adhesion in vitro. a , b A total of 3000 cells/well human lung fibroblasts were cultured a without or b with 5 mM <t>EDTA</t> in FGM-2 culture media containing 1% <t>FBS.</t> c , d A total of 3000 cells/well HUVEC were cultured c without or d with 5 mM EDTA in EBM-2 culture media containing 100 ng/mL VEGF-A165 and 1% FBS. Cells were plated on 1 μg/mL laminin peptide pre-coated non-tissue culture-treated plates and incubated for 30 min at 37 °C. After plate washes, cell numbers were quantified using a CyQUANT assay ( n = 10, mean ± SEM). The signals obtained from BSA-coated wells are normalized to 1, and relative fold increases of cell numbers were calculated. Statistical analyses were performed using ANOVA with Tukey’s test. Kruskal–Wallis test followed by Dunn’s multiple comparison was used in b , c . * p
    Ethylenediaminetetraacetic Acids, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Avantor ethylenediaminetetraacteic acid
    Laminin HBD peptides promote cell adhesion in vitro. a , b A total of 3000 cells/well human lung fibroblasts were cultured a without or b with 5 mM <t>EDTA</t> in FGM-2 culture media containing 1% <t>FBS.</t> c , d A total of 3000 cells/well HUVEC were cultured c without or d with 5 mM EDTA in EBM-2 culture media containing 100 ng/mL VEGF-A165 and 1% FBS. Cells were plated on 1 μg/mL laminin peptide pre-coated non-tissue culture-treated plates and incubated for 30 min at 37 °C. After plate washes, cell numbers were quantified using a CyQUANT assay ( n = 10, mean ± SEM). The signals obtained from BSA-coated wells are normalized to 1, and relative fold increases of cell numbers were calculated. Statistical analyses were performed using ANOVA with Tukey’s test. Kruskal–Wallis test followed by Dunn’s multiple comparison was used in b , c . * p
    Ethylenediaminetetraacteic Acid, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Roche ethylenediaminetetraacectic acid
    Laminin HBD peptides promote cell adhesion in vitro. a , b A total of 3000 cells/well human lung fibroblasts were cultured a without or b with 5 mM <t>EDTA</t> in FGM-2 culture media containing 1% <t>FBS.</t> c , d A total of 3000 cells/well HUVEC were cultured c without or d with 5 mM EDTA in EBM-2 culture media containing 100 ng/mL VEGF-A165 and 1% FBS. Cells were plated on 1 μg/mL laminin peptide pre-coated non-tissue culture-treated plates and incubated for 30 min at 37 °C. After plate washes, cell numbers were quantified using a CyQUANT assay ( n = 10, mean ± SEM). The signals obtained from BSA-coated wells are normalized to 1, and relative fold increases of cell numbers were calculated. Statistical analyses were performed using ANOVA with Tukey’s test. Kruskal–Wallis test followed by Dunn’s multiple comparison was used in b , c . * p
    Ethylenediaminetetraacectic Acid, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Millipore ethylenediaminetetraacetic acid solution
    Laminin HBD peptides promote cell adhesion in vitro. a , b A total of 3000 cells/well human lung fibroblasts were cultured a without or b with 5 mM <t>EDTA</t> in FGM-2 culture media containing 1% <t>FBS.</t> c , d A total of 3000 cells/well HUVEC were cultured c without or d with 5 mM EDTA in EBM-2 culture media containing 100 ng/mL VEGF-A165 and 1% FBS. Cells were plated on 1 μg/mL laminin peptide pre-coated non-tissue culture-treated plates and incubated for 30 min at 37 °C. After plate washes, cell numbers were quantified using a CyQUANT assay ( n = 10, mean ± SEM). The signals obtained from BSA-coated wells are normalized to 1, and relative fold increases of cell numbers were calculated. Statistical analyses were performed using ANOVA with Tukey’s test. Kruskal–Wallis test followed by Dunn’s multiple comparison was used in b , c . * p
    Ethylenediaminetetraacetic Acid Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore ethylenediaminetetraacetic acid tetrasodium salt hydrate
    Laminin HBD peptides promote cell adhesion in vitro. a , b A total of 3000 cells/well human lung fibroblasts were cultured a without or b with 5 mM <t>EDTA</t> in FGM-2 culture media containing 1% <t>FBS.</t> c , d A total of 3000 cells/well HUVEC were cultured c without or d with 5 mM EDTA in EBM-2 culture media containing 100 ng/mL VEGF-A165 and 1% FBS. Cells were plated on 1 μg/mL laminin peptide pre-coated non-tissue culture-treated plates and incubated for 30 min at 37 °C. After plate washes, cell numbers were quantified using a CyQUANT assay ( n = 10, mean ± SEM). The signals obtained from BSA-coated wells are normalized to 1, and relative fold increases of cell numbers were calculated. Statistical analyses were performed using ANOVA with Tukey’s test. Kruskal–Wallis test followed by Dunn’s multiple comparison was used in b , c . * p
    Ethylenediaminetetraacetic Acid Tetrasodium Salt Hydrate, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Amresco edta
    Laminin HBD peptides promote cell adhesion in vitro. a , b A total of 3000 cells/well human lung fibroblasts were cultured a without or b with 5 mM <t>EDTA</t> in FGM-2 culture media containing 1% <t>FBS.</t> c , d A total of 3000 cells/well HUVEC were cultured c without or d with 5 mM EDTA in EBM-2 culture media containing 100 ng/mL VEGF-A165 and 1% FBS. Cells were plated on 1 μg/mL laminin peptide pre-coated non-tissue culture-treated plates and incubated for 30 min at 37 °C. After plate washes, cell numbers were quantified using a CyQUANT assay ( n = 10, mean ± SEM). The signals obtained from BSA-coated wells are normalized to 1, and relative fold increases of cell numbers were calculated. Statistical analyses were performed using ANOVA with Tukey’s test. Kruskal–Wallis test followed by Dunn’s multiple comparison was used in b , c . * p
    Edta, supplied by Amresco, used in various techniques. Bioz Stars score: 92/100, based on 421 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Sinopharm ethylenediaminetetraacetic acid
    Laminin HBD peptides promote cell adhesion in vitro. a , b A total of 3000 cells/well human lung fibroblasts were cultured a without or b with 5 mM <t>EDTA</t> in FGM-2 culture media containing 1% <t>FBS.</t> c , d A total of 3000 cells/well HUVEC were cultured c without or d with 5 mM EDTA in EBM-2 culture media containing 100 ng/mL VEGF-A165 and 1% FBS. Cells were plated on 1 μg/mL laminin peptide pre-coated non-tissue culture-treated plates and incubated for 30 min at 37 °C. After plate washes, cell numbers were quantified using a CyQUANT assay ( n = 10, mean ± SEM). The signals obtained from BSA-coated wells are normalized to 1, and relative fold increases of cell numbers were calculated. Statistical analyses were performed using ANOVA with Tukey’s test. Kruskal–Wallis test followed by Dunn’s multiple comparison was used in b , c . * p
    Ethylenediaminetetraacetic Acid, supplied by Sinopharm, used in various techniques. Bioz Stars score: 92/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Mallinckrodt ethylenediaminetetraacetic acid
    Laminin HBD peptides promote cell adhesion in vitro. a , b A total of 3000 cells/well human lung fibroblasts were cultured a without or b with 5 mM <t>EDTA</t> in FGM-2 culture media containing 1% <t>FBS.</t> c , d A total of 3000 cells/well HUVEC were cultured c without or d with 5 mM EDTA in EBM-2 culture media containing 100 ng/mL VEGF-A165 and 1% FBS. Cells were plated on 1 μg/mL laminin peptide pre-coated non-tissue culture-treated plates and incubated for 30 min at 37 °C. After plate washes, cell numbers were quantified using a CyQUANT assay ( n = 10, mean ± SEM). The signals obtained from BSA-coated wells are normalized to 1, and relative fold increases of cell numbers were calculated. Statistical analyses were performed using ANOVA with Tukey’s test. Kruskal–Wallis test followed by Dunn’s multiple comparison was used in b , c . * p
    Ethylenediaminetetraacetic Acid, supplied by Mallinckrodt, used in various techniques. Bioz Stars score: 93/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    TaKaRa ethylenediaminetetraacetic acid
    Laminin HBD peptides promote cell adhesion in vitro. a , b A total of 3000 cells/well human lung fibroblasts were cultured a without or b with 5 mM <t>EDTA</t> in FGM-2 culture media containing 1% <t>FBS.</t> c , d A total of 3000 cells/well HUVEC were cultured c without or d with 5 mM EDTA in EBM-2 culture media containing 100 ng/mL VEGF-A165 and 1% FBS. Cells were plated on 1 μg/mL laminin peptide pre-coated non-tissue culture-treated plates and incubated for 30 min at 37 °C. After plate washes, cell numbers were quantified using a CyQUANT assay ( n = 10, mean ± SEM). The signals obtained from BSA-coated wells are normalized to 1, and relative fold increases of cell numbers were calculated. Statistical analyses were performed using ANOVA with Tukey’s test. Kruskal–Wallis test followed by Dunn’s multiple comparison was used in b , c . * p
    Ethylenediaminetetraacetic Acid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Biological Industries Inc edta
    Laminin HBD peptides promote cell adhesion in vitro. a , b A total of 3000 cells/well human lung fibroblasts were cultured a without or b with 5 mM <t>EDTA</t> in FGM-2 culture media containing 1% <t>FBS.</t> c , d A total of 3000 cells/well HUVEC were cultured c without or d with 5 mM EDTA in EBM-2 culture media containing 100 ng/mL VEGF-A165 and 1% FBS. Cells were plated on 1 μg/mL laminin peptide pre-coated non-tissue culture-treated plates and incubated for 30 min at 37 °C. After plate washes, cell numbers were quantified using a CyQUANT assay ( n = 10, mean ± SEM). The signals obtained from BSA-coated wells are normalized to 1, and relative fold increases of cell numbers were calculated. Statistical analyses were performed using ANOVA with Tukey’s test. Kruskal–Wallis test followed by Dunn’s multiple comparison was used in b , c . * p
    Edta, supplied by Biological Industries Inc, used in various techniques. Bioz Stars score: 92/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Fisher Scientific edta
    The effect of <t>EDTA</t> (left) and <t>EGTA</t> (right) on barnacle cement polymerization. Unpolymerized cement was incubated with EDTA, EGTA or deionized water for 2 min and then run on SDS-PAGE. Mean intensity (+s.e.m.) for the protein bands labeled in
    Edta, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 567 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nacalai edta
    Flow <t>cytometry</t> of hPDPN-transfected cells using anti-hPDPN antibodies LpMab-7 and chLpMab-7 Cells were harvested by brief exposure to 0.25% Trypsin/1 mM <t>EDTA.</t> After washing with PBS, the cells were treated with LpMab-7 A. and chLpMab-7 B. followed by treatment with secondary antibodies. Fluorescence data were collected using a Cell Analyzer EC800. C. The epitope mapping of chLpMab-7 by Western blot analysis. Total cell lysates of point mutants of hPDPN were electrophoresed on 5–20% polyacrylamide gels and transferred onto a PVDF membrane. After blocking, the membrane was incubated with 1 μg/ml mAbs (chLpMab-7 or NZ-8 against hPDPN, 1E5 against FLAG tag, AC-15 against β-actin) and then with peroxidase-conjugated secondary antibodies; the membrane was analyzed using a Sayaca-Imager.
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    90
    Becton Dickinson potassium edta
    Preparation of HDL associated <t>LCAT.</t> Size exclusion chromatography was perfo rmed at ambient temperature using a Superose-6 10/300 column on a Bio-Rad FPLC system (Hercules, CA). A 200 µl aliquot of plasma was injected per run, and eluted in PBS with 1 mM <t>EDTA</t> at a flow rate of 0.2 ml/min. Total cholesterol in each fraction was measured using the Cholesterol E Kit. LCAT activity was determined as following: A. 20 µl of each fraction of human plasma were incubated at 37ºC for 5 h; B. 2.5 µl of each fraction of WT mouse plasma were incubated at 37ºC for 2 h; C. 10 µl of each fraction of apoE-null mouse plasma were incubated at 37ºC for 4 h.
    Potassium Edta, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nanjing Chemical Reagent Co Ltd edta
    Cyclic voltammograms obtained at the <t>GSH</t> modified electrode in the presence of 1 μM Cu 2+ for 1 h. The GSH has been pre-incubated with 5 U/mL GGT at 37 °C (dash dot line) or 25 °C (dash line) for 30 min, or without GGT (solid line), and then incubated with a gold electrode for 16 h to form the GSH modified electrode. The dot line shows the case that <t>EDTA</t> instead of GGT is added in the test solution. Scan rate: 50 mV·s −1 .
    Edta, supplied by Nanjing Chemical Reagent Co Ltd, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    HiMedia Laboratories edta
    Cyclic voltammograms obtained at the <t>GSH</t> modified electrode in the presence of 1 μM Cu 2+ for 1 h. The GSH has been pre-incubated with 5 U/mL GGT at 37 °C (dash dot line) or 25 °C (dash line) for 30 min, or without GGT (solid line), and then incubated with a gold electrode for 16 h to form the GSH modified electrode. The dot line shows the case that <t>EDTA</t> instead of GGT is added in the test solution. Scan rate: 50 mV·s −1 .
    Edta, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Terumo Corp edta
    Cyclic voltammograms obtained at the <t>GSH</t> modified electrode in the presence of 1 μM Cu 2+ for 1 h. The GSH has been pre-incubated with 5 U/mL GGT at 37 °C (dash dot line) or 25 °C (dash line) for 30 min, or without GGT (solid line), and then incubated with a gold electrode for 16 h to form the GSH modified electrode. The dot line shows the case that <t>EDTA</t> instead of GGT is added in the test solution. Scan rate: 50 mV·s −1 .
    Edta, supplied by Terumo Corp, used in various techniques. Bioz Stars score: 93/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore ethylenediaminetetraacetic acid tetra na salt
    Cyclic voltammograms obtained at the <t>GSH</t> modified electrode in the presence of 1 μM Cu 2+ for 1 h. The GSH has been pre-incubated with 5 U/mL GGT at 37 °C (dash dot line) or 25 °C (dash line) for 30 min, or without GGT (solid line), and then incubated with a gold electrode for 16 h to form the GSH modified electrode. The dot line shows the case that <t>EDTA</t> instead of GGT is added in the test solution. Scan rate: 50 mV·s −1 .
    Ethylenediaminetetraacetic Acid Tetra Na Salt, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Avantor edta
    Cyclic voltammograms obtained at the <t>GSH</t> modified electrode in the presence of 1 μM Cu 2+ for 1 h. The GSH has been pre-incubated with 5 U/mL GGT at 37 °C (dash dot line) or 25 °C (dash line) for 30 min, or without GGT (solid line), and then incubated with a gold electrode for 16 h to form the GSH modified electrode. The dot line shows the case that <t>EDTA</t> instead of GGT is added in the test solution. Scan rate: 50 mV·s −1 .
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    edta  (Roche)
    94
    Roche edta
    Cyclic voltammograms obtained at the <t>GSH</t> modified electrode in the presence of 1 μM Cu 2+ for 1 h. The GSH has been pre-incubated with 5 U/mL GGT at 37 °C (dash dot line) or 25 °C (dash line) for 30 min, or without GGT (solid line), and then incubated with a gold electrode for 16 h to form the GSH modified electrode. The dot line shows the case that <t>EDTA</t> instead of GGT is added in the test solution. Scan rate: 50 mV·s −1 .
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    Image Search Results


    Transport of catechin, EGCG, and their niosomes by human intestinal Caco-2 cells. Notes: Schematic diagram of transcellular transport and different transporters in Caco-2 cells ( A ); effect of concentration on the flux of catechin, EGCG, and their niosomes (0, 10, 20, 50, and 100 μg/mL) through Caco-2 monolayer from apical to basolateral chamber ( B ); effect of temperature (4°C and 37°C), ATP inhibitor (sodium azide), P-gp inhibitor (verapamil), MRP2 inhibitor (MK-571) and absorption enhancer (EDTA) on transport of 100 μg/mL different drugs after 6 hours ( C ). Data are presented as mean ± SD (n=3). Abbreviations: EDTA, ethylenediaminetetraacetic acid; EGCG, (−)-epigallocatechin gallate; MRP2, multidrug resistance-associated protein 2; P-gp, permeability glycoprotein; SD, standard deviation.

    Journal: International Journal of Nanomedicine

    Article Title: Enhanced uptake and transport of (+)-catechin and (-)-epigallocatechin gallate in niosomal formulation by human intestinal Caco-2 cells

    doi: 10.2147/IJN.S59331

    Figure Lengend Snippet: Transport of catechin, EGCG, and their niosomes by human intestinal Caco-2 cells. Notes: Schematic diagram of transcellular transport and different transporters in Caco-2 cells ( A ); effect of concentration on the flux of catechin, EGCG, and their niosomes (0, 10, 20, 50, and 100 μg/mL) through Caco-2 monolayer from apical to basolateral chamber ( B ); effect of temperature (4°C and 37°C), ATP inhibitor (sodium azide), P-gp inhibitor (verapamil), MRP2 inhibitor (MK-571) and absorption enhancer (EDTA) on transport of 100 μg/mL different drugs after 6 hours ( C ). Data are presented as mean ± SD (n=3). Abbreviations: EDTA, ethylenediaminetetraacetic acid; EGCG, (−)-epigallocatechin gallate; MRP2, multidrug resistance-associated protein 2; P-gp, permeability glycoprotein; SD, standard deviation.

    Article Snippet: Chemicals and reagents Catechin, EGCG, sorbitan monostearate (Span 60), CH, dihexadecyl phosphate, fluorescein isothiocyanate (FITC), sulforhodamine B (SRB), fluorescein sodium salt, ascorbic acid (AA), sodium azide, verapamil, 5-(3-(2-(7-chloroquinolin-2-yl)ethenyl)phenyl)-8-dimethylcarbamyl-4,6-dithiaoctanoic acid sodium salt hydrate (MK-571), and ethylenediaminetetraacetic acid (EDTA) were purchased from Sigma-Aldrich (St Louis, MO, USA).

    Techniques: Concentration Assay, Permeability, Standard Deviation

    Laminin HBD peptides promote cell adhesion in vitro. a , b A total of 3000 cells/well human lung fibroblasts were cultured a without or b with 5 mM EDTA in FGM-2 culture media containing 1% FBS. c , d A total of 3000 cells/well HUVEC were cultured c without or d with 5 mM EDTA in EBM-2 culture media containing 100 ng/mL VEGF-A165 and 1% FBS. Cells were plated on 1 μg/mL laminin peptide pre-coated non-tissue culture-treated plates and incubated for 30 min at 37 °C. After plate washes, cell numbers were quantified using a CyQUANT assay ( n = 10, mean ± SEM). The signals obtained from BSA-coated wells are normalized to 1, and relative fold increases of cell numbers were calculated. Statistical analyses were performed using ANOVA with Tukey’s test. Kruskal–Wallis test followed by Dunn’s multiple comparison was used in b , c . * p

    Journal: Nature Communications

    Article Title: Laminin heparin-binding peptides bind to several growth factors and enhance diabetic wound healing

    doi: 10.1038/s41467-018-04525-w

    Figure Lengend Snippet: Laminin HBD peptides promote cell adhesion in vitro. a , b A total of 3000 cells/well human lung fibroblasts were cultured a without or b with 5 mM EDTA in FGM-2 culture media containing 1% FBS. c , d A total of 3000 cells/well HUVEC were cultured c without or d with 5 mM EDTA in EBM-2 culture media containing 100 ng/mL VEGF-A165 and 1% FBS. Cells were plated on 1 μg/mL laminin peptide pre-coated non-tissue culture-treated plates and incubated for 30 min at 37 °C. After plate washes, cell numbers were quantified using a CyQUANT assay ( n = 10, mean ± SEM). The signals obtained from BSA-coated wells are normalized to 1, and relative fold increases of cell numbers were calculated. Statistical analyses were performed using ANOVA with Tukey’s test. Kruskal–Wallis test followed by Dunn’s multiple comparison was used in b , c . * p

    Article Snippet: Cell adhesion assays were performed using human lung fibroblasts (Lonza) in FGM-2 medium (Lonza) or human umbilical vein endothelial cells (HUVEC; Lonza) in EGM-2 medium (Lonza) supplemented with 1% fetal bovine serum (FBS) and 100 µg/mL VEGF-A165, with or without 5 mM EDTA (Sigma-Aldrich).

    Techniques: In Vitro, Cell Culture, Incubation, CyQUANT Assay

    The effect of EDTA (left) and EGTA (right) on barnacle cement polymerization. Unpolymerized cement was incubated with EDTA, EGTA or deionized water for 2 min and then run on SDS-PAGE. Mean intensity (+s.e.m.) for the protein bands labeled in

    Journal: The Journal of Experimental Biology

    Article Title: Barnacle cement: a polymerization model based on evolutionary concepts

    doi: 10.1242/jeb.029884

    Figure Lengend Snippet: The effect of EDTA (left) and EGTA (right) on barnacle cement polymerization. Unpolymerized cement was incubated with EDTA, EGTA or deionized water for 2 min and then run on SDS-PAGE. Mean intensity (+s.e.m.) for the protein bands labeled in

    Article Snippet: EGTA (Sigma-Aldrich no. E4378) and EDTA (Fisher Chemical, no. BP118) were used.

    Techniques: Incubation, SDS Page, Labeling

    Flow cytometry of hPDPN-transfected cells using anti-hPDPN antibodies LpMab-7 and chLpMab-7 Cells were harvested by brief exposure to 0.25% Trypsin/1 mM EDTA. After washing with PBS, the cells were treated with LpMab-7 A. and chLpMab-7 B. followed by treatment with secondary antibodies. Fluorescence data were collected using a Cell Analyzer EC800. C. The epitope mapping of chLpMab-7 by Western blot analysis. Total cell lysates of point mutants of hPDPN were electrophoresed on 5–20% polyacrylamide gels and transferred onto a PVDF membrane. After blocking, the membrane was incubated with 1 μg/ml mAbs (chLpMab-7 or NZ-8 against hPDPN, 1E5 against FLAG tag, AC-15 against β-actin) and then with peroxidase-conjugated secondary antibodies; the membrane was analyzed using a Sayaca-Imager.

    Journal: Oncotarget

    Article Title: The chimeric antibody chLpMab-7 targeting human podoplanin suppresses pulmonary metastasis via ADCC and CDC rather than via its neutralizing activity

    doi:

    Figure Lengend Snippet: Flow cytometry of hPDPN-transfected cells using anti-hPDPN antibodies LpMab-7 and chLpMab-7 Cells were harvested by brief exposure to 0.25% Trypsin/1 mM EDTA. After washing with PBS, the cells were treated with LpMab-7 A. and chLpMab-7 B. followed by treatment with secondary antibodies. Fluorescence data were collected using a Cell Analyzer EC800. C. The epitope mapping of chLpMab-7 by Western blot analysis. Total cell lysates of point mutants of hPDPN were electrophoresed on 5–20% polyacrylamide gels and transferred onto a PVDF membrane. After blocking, the membrane was incubated with 1 μg/ml mAbs (chLpMab-7 or NZ-8 against hPDPN, 1E5 against FLAG tag, AC-15 against β-actin) and then with peroxidase-conjugated secondary antibodies; the membrane was analyzed using a Sayaca-Imager.

    Article Snippet: Flow cytometry Cells were harvested by brief exposure to 0.25% Trypsin/1 mM EDTA (Nacalai Tesque, Inc.).

    Techniques: Flow Cytometry, Cytometry, Transfection, Fluorescence, Western Blot, Blocking Assay, Incubation, FLAG-tag

    Flow cytometry of endogenous hPDPN-expressing cell lines using anti-hPDPN antibodies LpMab-7 and chLpMab-7 Cancer cells A. or normal cells B. were harvested by brief exposure to 0.25% Trypsin/1 mM EDTA. After washing with PBS, the cells were treated with LpMab-7 and chLpMab-7 followed by treatment with secondary antibodies. Fluorescence data were collected using a Cell Analyzer EC800.

    Journal: Oncotarget

    Article Title: The chimeric antibody chLpMab-7 targeting human podoplanin suppresses pulmonary metastasis via ADCC and CDC rather than via its neutralizing activity

    doi:

    Figure Lengend Snippet: Flow cytometry of endogenous hPDPN-expressing cell lines using anti-hPDPN antibodies LpMab-7 and chLpMab-7 Cancer cells A. or normal cells B. were harvested by brief exposure to 0.25% Trypsin/1 mM EDTA. After washing with PBS, the cells were treated with LpMab-7 and chLpMab-7 followed by treatment with secondary antibodies. Fluorescence data were collected using a Cell Analyzer EC800.

    Article Snippet: Flow cytometry Cells were harvested by brief exposure to 0.25% Trypsin/1 mM EDTA (Nacalai Tesque, Inc.).

    Techniques: Flow Cytometry, Cytometry, Expressing, Fluorescence

    Preparation of HDL associated LCAT. Size exclusion chromatography was perfo rmed at ambient temperature using a Superose-6 10/300 column on a Bio-Rad FPLC system (Hercules, CA). A 200 µl aliquot of plasma was injected per run, and eluted in PBS with 1 mM EDTA at a flow rate of 0.2 ml/min. Total cholesterol in each fraction was measured using the Cholesterol E Kit. LCAT activity was determined as following: A. 20 µl of each fraction of human plasma were incubated at 37ºC for 5 h; B. 2.5 µl of each fraction of WT mouse plasma were incubated at 37ºC for 2 h; C. 10 µl of each fraction of apoE-null mouse plasma were incubated at 37ºC for 4 h.

    Journal: International Journal of Biological Sciences

    Article Title: An apoA-I mimetic peptide increases LCAT activity in mice through increasing HDL concentration

    doi:

    Figure Lengend Snippet: Preparation of HDL associated LCAT. Size exclusion chromatography was perfo rmed at ambient temperature using a Superose-6 10/300 column on a Bio-Rad FPLC system (Hercules, CA). A 200 µl aliquot of plasma was injected per run, and eluted in PBS with 1 mM EDTA at a flow rate of 0.2 ml/min. Total cholesterol in each fraction was measured using the Cholesterol E Kit. LCAT activity was determined as following: A. 20 µl of each fraction of human plasma were incubated at 37ºC for 5 h; B. 2.5 µl of each fraction of WT mouse plasma were incubated at 37ºC for 2 h; C. 10 µl of each fraction of apoE-null mouse plasma were incubated at 37ºC for 4 h.

    Article Snippet: Preparation of HDL associated LCAT enzyme Fresh human or mouse blood was drawn into tubes containing potassium EDTA (Becton Dickinson, Franklin lakes, NJ), and plasma was separated immediately by low-speed centrifugation at 2500 rpm (1430g ) for 30 min at 4ºC.

    Techniques: Size-exclusion Chromatography, Fast Protein Liquid Chromatography, Injection, Flow Cytometry, Activity Assay, Incubation

    Cyclic voltammograms obtained at the GSH modified electrode in the presence of 1 μM Cu 2+ for 1 h. The GSH has been pre-incubated with 5 U/mL GGT at 37 °C (dash dot line) or 25 °C (dash line) for 30 min, or without GGT (solid line), and then incubated with a gold electrode for 16 h to form the GSH modified electrode. The dot line shows the case that EDTA instead of GGT is added in the test solution. Scan rate: 50 mV·s −1 .

    Journal: International Journal of Molecular Sciences

    Article Title: An Electrochemical Method to Detect Gamma Glutamyl Transpeptidase

    doi: 10.3390/ijms13032801

    Figure Lengend Snippet: Cyclic voltammograms obtained at the GSH modified electrode in the presence of 1 μM Cu 2+ for 1 h. The GSH has been pre-incubated with 5 U/mL GGT at 37 °C (dash dot line) or 25 °C (dash line) for 30 min, or without GGT (solid line), and then incubated with a gold electrode for 16 h to form the GSH modified electrode. The dot line shows the case that EDTA instead of GGT is added in the test solution. Scan rate: 50 mV·s −1 .

    Article Snippet: GSH was purchased from Nanjing Dazhi Biotechnology Co., Ltd. CuSO4 , and EDTA were acquired from Nanjing Chemical Reagent Co., Ltd, which were both of analytical grade.

    Techniques: Modification, Incubation