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  • 99
    Thermo Fisher ethylenediaminetetraacetic acid
    The preparation of B6 epithelium (EPI) p35 + B6 stroma-endothelium (S-E) composite graft and transplantation model. Harvested B6 corneas were incubated with pHAGE-CMV-p35-IZsGreenW lentiviral vector (3 × 10 5 IU/mL) in the presence of hexadimethrine bromide for 1 h. Transduced corneal layers were treated with <t>ethylenediaminetetraacetic</t> acid for 45 min, and subsequently the intact sheet of epithelium was peeled off and transplanted on untreated S-E of B6 (a) . The photos illustrate the separation of the epithelial layer and the replacement of p35 -treated epithelium on S-E of the same strain (b) . Transduction efficacy in the epithelium was confirmed by detection of IZsGreenW in confocal fluorescence microscopy. Murine corneas were successfully transduced with p35 (visible IZsGreenW expressing cells in green and nuclei in blue ) and untreated negative control (visible nuclei) (c) . The composite grafts were transplanted in Balb/c (B/c) mice (day 0) (d) . B/c mice as recipients were transplanted with B/c EPI + B/c S-E ( n = 5), B6 EPI + B6 S-E ( n = 5), B6 EPI empty + B6 S-E ( n = 4), and B6 EPI p35 + B6 S-E ( n = 4) composite grafts (e)
    Ethylenediaminetetraacetic Acid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1547 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore ethylenediaminetetraacetic acid
    Dependence of pH and pCu fixed (the negative logarithm of the concentration of Cu 2+ ions fixed by chelating agent) on the Cu 2+ to chelating agent molar ratio (n Cu :n chelating agent ) in water ( a , c , e ) and KCl solution ( b , d , f ) at pH 5.5; ( a , b ) rhamnolipids (Rh); ( c , d ) methylglycinediacetic acid (MGDA); and ( e , f ) <t>ethylenediaminetetraacetic</t> acid (EDTA); CMC Rh means the rhamnolipids’ critical micelle concentration.
    Ethylenediaminetetraacetic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 4360 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore ethylenediaminetetraacetic acid edta
    Transport of catechin, EGCG, and their niosomes by human intestinal Caco-2 cells. Notes: Schematic diagram of transcellular transport and different transporters in Caco-2 cells ( A ); effect of concentration on the flux of catechin, EGCG, and their niosomes (0, 10, 20, 50, and 100 μg/mL) through Caco-2 monolayer from apical to basolateral chamber ( B ); effect of temperature (4°C and 37°C), ATP inhibitor (sodium azide), P-gp inhibitor (verapamil), MRP2 inhibitor (MK-571) and absorption enhancer <t>(EDTA)</t> on transport of 100 μg/mL different drugs after 6 hours ( C ). Data are presented as mean ± SD (n=3). Abbreviations: EDTA, <t>ethylenediaminetetraacetic</t> acid; EGCG, (−)-epigallocatechin gallate; MRP2, multidrug resistance-associated protein 2; P-gp, permeability glycoprotein; SD, standard deviation.
    Ethylenediaminetetraacetic Acid Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 19798 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher trypsin ethylenediaminetetraacetic acid
    Transport of catechin, EGCG, and their niosomes by human intestinal Caco-2 cells. Notes: Schematic diagram of transcellular transport and different transporters in Caco-2 cells ( A ); effect of concentration on the flux of catechin, EGCG, and their niosomes (0, 10, 20, 50, and 100 μg/mL) through Caco-2 monolayer from apical to basolateral chamber ( B ); effect of temperature (4°C and 37°C), ATP inhibitor (sodium azide), P-gp inhibitor (verapamil), MRP2 inhibitor (MK-571) and absorption enhancer <t>(EDTA)</t> on transport of 100 μg/mL different drugs after 6 hours ( C ). Data are presented as mean ± SD (n=3). Abbreviations: EDTA, <t>ethylenediaminetetraacetic</t> acid; EGCG, (−)-epigallocatechin gallate; MRP2, multidrug resistance-associated protein 2; P-gp, permeability glycoprotein; SD, standard deviation.
    Trypsin Ethylenediaminetetraacetic Acid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 2105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher ethylenediaminetetraacetic acid edta
    Transport of catechin, EGCG, and their niosomes by human intestinal Caco-2 cells. Notes: Schematic diagram of transcellular transport and different transporters in Caco-2 cells ( A ); effect of concentration on the flux of catechin, EGCG, and their niosomes (0, 10, 20, 50, and 100 μg/mL) through Caco-2 monolayer from apical to basolateral chamber ( B ); effect of temperature (4°C and 37°C), ATP inhibitor (sodium azide), P-gp inhibitor (verapamil), MRP2 inhibitor (MK-571) and absorption enhancer <t>(EDTA)</t> on transport of 100 μg/mL different drugs after 6 hours ( C ). Data are presented as mean ± SD (n=3). Abbreviations: EDTA, <t>ethylenediaminetetraacetic</t> acid; EGCG, (−)-epigallocatechin gallate; MRP2, multidrug resistance-associated protein 2; P-gp, permeability glycoprotein; SD, standard deviation.
    Ethylenediaminetetraacetic Acid Edta, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1217 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson ethylenediaminetetraacetic acid
    Impact of different blood anticoagulants on the surface expression of mCD14 on bovine monocytes . Whole blood samples obtained from 18 Holstein cows were anticoagulated with: sodium heparin (HEPARIN), sodium citrate (CITRATE) and <t>ethylenediaminetetraacetic</t> acid <t>(EDTA).</t> The percentage of monocytes expressing mCD14 (2A) and the mean channel fluorescence intensity (MFI) (2B) was measured. Results for each treatment are the mean from 6 cows. Treatment means with different superscripts are significantly different using Scheffe's multiple comparison tests.
    Ethylenediaminetetraacetic Acid, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 428 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore trypsin ethylenediaminetetraacetic acid
    Impact of different blood anticoagulants on the surface expression of mCD14 on bovine monocytes . Whole blood samples obtained from 18 Holstein cows were anticoagulated with: sodium heparin (HEPARIN), sodium citrate (CITRATE) and <t>ethylenediaminetetraacetic</t> acid <t>(EDTA).</t> The percentage of monocytes expressing mCD14 (2A) and the mean channel fluorescence intensity (MFI) (2B) was measured. Results for each treatment are the mean from 6 cows. Treatment means with different superscripts are significantly different using Scheffe's multiple comparison tests.
    Trypsin Ethylenediaminetetraacetic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 310 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher ethylenediaminetetraacetic acid edta 0 5
    Impact of different blood anticoagulants on the surface expression of mCD14 on bovine monocytes . Whole blood samples obtained from 18 Holstein cows were anticoagulated with: sodium heparin (HEPARIN), sodium citrate (CITRATE) and <t>ethylenediaminetetraacetic</t> acid <t>(EDTA).</t> The percentage of monocytes expressing mCD14 (2A) and the mean channel fluorescence intensity (MFI) (2B) was measured. Results for each treatment are the mean from 6 cows. Treatment means with different superscripts are significantly different using Scheffe's multiple comparison tests.
    Ethylenediaminetetraacetic Acid Edta 0 5, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA ethylenediaminetetraacetic acid edta
    Scanning electron micrographs (a) 1% oregano extract solution (OES) + 17% <t>ethylenediaminetetraacetic</t> acid <t>(EDTA)</t> (b) 2% OES + 17% EDTA (c) 5% OES + 17% EDTA
    Ethylenediaminetetraacetic Acid Edta, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fisher Scientific ethylenediaminetetraacetic acid edta
    Scanning electron micrographs (a) 1% oregano extract solution (OES) + 17% <t>ethylenediaminetetraacetic</t> acid <t>(EDTA)</t> (b) 2% OES + 17% EDTA (c) 5% OES + 17% EDTA
    Ethylenediaminetetraacetic Acid Edta, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher trypsin ethylenediaminetetraacetic acid edta solution
    Scanning electron micrographs (a) 1% oregano extract solution (OES) + 17% <t>ethylenediaminetetraacetic</t> acid <t>(EDTA)</t> (b) 2% OES + 17% EDTA (c) 5% OES + 17% EDTA
    Trypsin Ethylenediaminetetraacetic Acid Edta Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 238 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sarstedt ethylenediaminetetraacetic acid
    Scanning electron micrographs (a) 1% oregano extract solution (OES) + 17% <t>ethylenediaminetetraacetic</t> acid <t>(EDTA)</t> (b) 2% OES + 17% EDTA (c) 5% OES + 17% EDTA
    Ethylenediaminetetraacetic Acid, supplied by Sarstedt, used in various techniques. Bioz Stars score: 93/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore ethylenediaminetetraacetic acid disodium salt dihydrate
    Scanning electron micrographs (a) 1% oregano extract solution (OES) + 17% <t>ethylenediaminetetraacetic</t> acid <t>(EDTA)</t> (b) 2% OES + 17% EDTA (c) 5% OES + 17% EDTA
    Ethylenediaminetetraacetic Acid Disodium Salt Dihydrate, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    FUJIFILM ethylenediaminetetraacetic acid edta
    Scanning electron micrographs (a) 1% oregano extract solution (OES) + 17% <t>ethylenediaminetetraacetic</t> acid <t>(EDTA)</t> (b) 2% OES + 17% EDTA (c) 5% OES + 17% EDTA
    Ethylenediaminetetraacetic Acid Edta, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 92/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson ethylenediaminetetraacetic acid edta tubes
    Scanning electron micrographs (a) 1% oregano extract solution (OES) + 17% <t>ethylenediaminetetraacetic</t> acid <t>(EDTA)</t> (b) 2% OES + 17% EDTA (c) 5% OES + 17% EDTA
    Ethylenediaminetetraacetic Acid Edta Tubes, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    The preparation of B6 epithelium (EPI) p35 + B6 stroma-endothelium (S-E) composite graft and transplantation model. Harvested B6 corneas were incubated with pHAGE-CMV-p35-IZsGreenW lentiviral vector (3 × 10 5 IU/mL) in the presence of hexadimethrine bromide for 1 h. Transduced corneal layers were treated with ethylenediaminetetraacetic acid for 45 min, and subsequently the intact sheet of epithelium was peeled off and transplanted on untreated S-E of B6 (a) . The photos illustrate the separation of the epithelial layer and the replacement of p35 -treated epithelium on S-E of the same strain (b) . Transduction efficacy in the epithelium was confirmed by detection of IZsGreenW in confocal fluorescence microscopy. Murine corneas were successfully transduced with p35 (visible IZsGreenW expressing cells in green and nuclei in blue ) and untreated negative control (visible nuclei) (c) . The composite grafts were transplanted in Balb/c (B/c) mice (day 0) (d) . B/c mice as recipients were transplanted with B/c EPI + B/c S-E ( n = 5), B6 EPI + B6 S-E ( n = 5), B6 EPI empty + B6 S-E ( n = 4), and B6 EPI p35 + B6 S-E ( n = 4) composite grafts (e)

    Journal: Human Gene Therapy

    Article Title: Gene Therapy for Modulation of T-Cell-Mediated Immune Response Provoked by Corneal Transplantation

    doi: 10.1089/hum.2017.044

    Figure Lengend Snippet: The preparation of B6 epithelium (EPI) p35 + B6 stroma-endothelium (S-E) composite graft and transplantation model. Harvested B6 corneas were incubated with pHAGE-CMV-p35-IZsGreenW lentiviral vector (3 × 10 5 IU/mL) in the presence of hexadimethrine bromide for 1 h. Transduced corneal layers were treated with ethylenediaminetetraacetic acid for 45 min, and subsequently the intact sheet of epithelium was peeled off and transplanted on untreated S-E of B6 (a) . The photos illustrate the separation of the epithelial layer and the replacement of p35 -treated epithelium on S-E of the same strain (b) . Transduction efficacy in the epithelium was confirmed by detection of IZsGreenW in confocal fluorescence microscopy. Murine corneas were successfully transduced with p35 (visible IZsGreenW expressing cells in green and nuclei in blue ) and untreated negative control (visible nuclei) (c) . The composite grafts were transplanted in Balb/c (B/c) mice (day 0) (d) . B/c mice as recipients were transplanted with B/c EPI + B/c S-E ( n = 5), B6 EPI + B6 S-E ( n = 5), B6 EPI empty + B6 S-E ( n = 4), and B6 EPI p35 + B6 S-E ( n = 4) composite grafts (e)

    Article Snippet: After determination of best transduction conditions, vectors were utilized at a concentration of 3 × 105 IU/mL applied in Roswell Park Memorial Institute (RPMI) medium (Thermo Fisher Scientific, Carlsbad, CA) containing 5% fetal calf serum (FCS; PAA, Linz, Austria) and hexadimethrine bromide (8 μg/mL; Sigma–Aldrich, St. Louis MO) at 37°C for 1 h. The transduced corneas were incubated in 20 mM of ethylenediaminetetraacetic acid (Thermo Fisher Scientific) for 45 min, and thereafter the epithelium was removed and placed immediately on S-E obtained from the same strain.

    Techniques: Transplantation Assay, Incubation, Plasmid Preparation, Transduction, Fluorescence, Microscopy, Expressing, Negative Control, Mouse Assay

    In vitro characterization of neuronal cell sheet derived from human induced pluripotent stem cells (hiPSCs). (A) A schematic representation of neuronal sheet formation from hiPSCs and transplantation. Embryoid bodies were developed from undifferentiated hiPSCs in a floating condition for 4 d. Then the cells were cultured in fibronectin-coated dishes for 4 d during which retinoic acid (RA), noggin (NOG), and cyclopamine (CyP; 3 factors) were introduced twice (on days 5 and 7). In some experiments, sonic hedgehog (SHH) were introduced instead of adding CyP, together with RA and NOG in order to compare the effects of CyP with those of SHH. Thereafter, the cells cultured with RA, NOG, and CyP were harvested on day 8. An aliquot of the cells was cultured in 24-well culture plates for 12 to 16 d and then harvested using trypsin/ethylenediaminetetraacetic acid (EDTA) solution for transplantation. The remaining cells were cultured on temperature-responsive gelation polymer-coated plate (temp. resp. polymer) for 12 to 16 d depending on the neuronal maturation, where they extended axon-like processes, leading to the formation of neuronal cell sheet. The neuronal cell sheet was recovered by lowering the temperature of culture plate below 22°C. Schedule for brain injury, transplantation, and motor function test was reported previously. 10 , 20 , 28 , 29 The cells on day 8 were used as neural stem/progenitor cells for comparison. (B) Stereomicroscopic view of the neuronal cell sheet cultured in a 48-well (diameter 12 mm) culture plate. Right lower part of the sheet detached from the bottom of the plate. (C) Stereomicroscopic view of the sheet in a 10-cm culture dish (diameter 90 mm). (D, E) Hematoxylin and eosin staining of the neuronal cell sheet (D, lower; E, higher magnification). Flow cytometric analysis of the cells in the sheet treated with trypsin/EDTA revealed that more than 75% of the cells expressed human NCAM (data not shown). (F, G) The neuronal cell sheet at day 19 was stained with anti-human nuclei (F) and anti-β-tubulin antibodies (G). (H) Real-time polymerase chain reaction analyses of the Figure 1. (continued) . neuronal cell sheet. For comparison, neural cells on day 8 (neural cells were cultured in the same manner except that SHH was added instead of CyP and were harvested on day 8 before making cell sheet) were included. The cell sheet expressed Foxp2, Fezf2, Igfbp4, CTIP2, and synaptophysin mRNA predominantly. CRIM1 mRNA was expressed throughout the culture period. Thus, the cell sheet expressed mRNAs of cortical motoneuron–associated proteins. A white horizontal bar represents 200 μm in panel D, 100 μm in panels E and F, and 50 μm in panel G. UiPSCs, undifferentiated-induced pluripotent stem cells; CRIM1, cysteine-rich motor neuron 1 protein precursor; MEF, mouse embryonic fibroblasts; floating indicates floating condition; Foxp2, forkhead box p2; Fezf2, forebrain embryonic zinc finger family zinc finger 2; Igfbp4, insulin-like growth factor-binding protein 4; CTIP2, COUP-TF-interacting protein 2; mRNA, messenger RNA.

    Journal: Cell Transplantation

    Article Title: Neuronal Cell Sheets of Cortical Motor Neuron Phenotype Derived from Human iPSCs

    doi: 10.1177/0963689717720280

    Figure Lengend Snippet: In vitro characterization of neuronal cell sheet derived from human induced pluripotent stem cells (hiPSCs). (A) A schematic representation of neuronal sheet formation from hiPSCs and transplantation. Embryoid bodies were developed from undifferentiated hiPSCs in a floating condition for 4 d. Then the cells were cultured in fibronectin-coated dishes for 4 d during which retinoic acid (RA), noggin (NOG), and cyclopamine (CyP; 3 factors) were introduced twice (on days 5 and 7). In some experiments, sonic hedgehog (SHH) were introduced instead of adding CyP, together with RA and NOG in order to compare the effects of CyP with those of SHH. Thereafter, the cells cultured with RA, NOG, and CyP were harvested on day 8. An aliquot of the cells was cultured in 24-well culture plates for 12 to 16 d and then harvested using trypsin/ethylenediaminetetraacetic acid (EDTA) solution for transplantation. The remaining cells were cultured on temperature-responsive gelation polymer-coated plate (temp. resp. polymer) for 12 to 16 d depending on the neuronal maturation, where they extended axon-like processes, leading to the formation of neuronal cell sheet. The neuronal cell sheet was recovered by lowering the temperature of culture plate below 22°C. Schedule for brain injury, transplantation, and motor function test was reported previously. 10 , 20 , 28 , 29 The cells on day 8 were used as neural stem/progenitor cells for comparison. (B) Stereomicroscopic view of the neuronal cell sheet cultured in a 48-well (diameter 12 mm) culture plate. Right lower part of the sheet detached from the bottom of the plate. (C) Stereomicroscopic view of the sheet in a 10-cm culture dish (diameter 90 mm). (D, E) Hematoxylin and eosin staining of the neuronal cell sheet (D, lower; E, higher magnification). Flow cytometric analysis of the cells in the sheet treated with trypsin/EDTA revealed that more than 75% of the cells expressed human NCAM (data not shown). (F, G) The neuronal cell sheet at day 19 was stained with anti-human nuclei (F) and anti-β-tubulin antibodies (G). (H) Real-time polymerase chain reaction analyses of the Figure 1. (continued) . neuronal cell sheet. For comparison, neural cells on day 8 (neural cells were cultured in the same manner except that SHH was added instead of CyP and were harvested on day 8 before making cell sheet) were included. The cell sheet expressed Foxp2, Fezf2, Igfbp4, CTIP2, and synaptophysin mRNA predominantly. CRIM1 mRNA was expressed throughout the culture period. Thus, the cell sheet expressed mRNAs of cortical motoneuron–associated proteins. A white horizontal bar represents 200 μm in panel D, 100 μm in panels E and F, and 50 μm in panel G. UiPSCs, undifferentiated-induced pluripotent stem cells; CRIM1, cysteine-rich motor neuron 1 protein precursor; MEF, mouse embryonic fibroblasts; floating indicates floating condition; Foxp2, forkhead box p2; Fezf2, forebrain embryonic zinc finger family zinc finger 2; Igfbp4, insulin-like growth factor-binding protein 4; CTIP2, COUP-TF-interacting protein 2; mRNA, messenger RNA.

    Article Snippet: An aliquot of the cells was cultured in 24-well culture plates for 12–16 d, which were then harvested using trypsin/ethylenediaminetetraacetic acid (EDTA) (Thermo Fisher Scientific, Inc.) solution for transplantation.

    Techniques: In Vitro, Derivative Assay, Transplantation Assay, Cell Culture, Staining, Flow Cytometry, Real-time Polymerase Chain Reaction, Binding Assay

    Dependence of pH and pCu fixed (the negative logarithm of the concentration of Cu 2+ ions fixed by chelating agent) on the Cu 2+ to chelating agent molar ratio (n Cu :n chelating agent ) in water ( a , c , e ) and KCl solution ( b , d , f ) at pH 5.5; ( a , b ) rhamnolipids (Rh); ( c , d ) methylglycinediacetic acid (MGDA); and ( e , f ) ethylenediaminetetraacetic acid (EDTA); CMC Rh means the rhamnolipids’ critical micelle concentration.

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: An Interaction of Rhamnolipids with Cu2+ Ions

    doi: 10.3390/molecules23020488

    Figure Lengend Snippet: Dependence of pH and pCu fixed (the negative logarithm of the concentration of Cu 2+ ions fixed by chelating agent) on the Cu 2+ to chelating agent molar ratio (n Cu :n chelating agent ) in water ( a , c , e ) and KCl solution ( b , d , f ) at pH 5.5; ( a , b ) rhamnolipids (Rh); ( c , d ) methylglycinediacetic acid (MGDA); and ( e , f ) ethylenediaminetetraacetic acid (EDTA); CMC Rh means the rhamnolipids’ critical micelle concentration.

    Article Snippet: The Chemicals Used The rhamnolipid R-95 (the 1:1 mixture of mono- and dirhamnolipids with the HPLC purity; the mean molecular weight of 577 g mol−1 , pK a = 5.6), methylglycinediacetic acid (MGDA; analytical grade, pK a = 14.6 [ ]), ethylenediaminetetraacetic acid (EDTA; analytical grade), potassium chloride (KCl), copper (II) sulfate (CuSO4 ; analytical grade), potassium hydroxide (KOH; analytical grade), and sulfuric acid (H2 SO4 ) were purchased from Sigma Aldrich (Poznan, Poland) company.

    Techniques: Concentration Assay

    Dependence of pH and pCu fixed (the negative logarithm of the concentration of Cu 2+ ions fixed by chelating agent) on the Cu 2+ to chelating agent molar ratio (n Cu :n chelating agent ) in water ( a , c , e ) and KCl solution ( b , d , f ) at pH 6.0; ( a , b ) rhamnolipids (Rh); ( c , d ) methylglycinediacetic acid (MGDA); and ( e , f ) ethylenediaminetetraacetic acid (EDTA); CMC Rh means the rhamnolipids’ critical micelle concentration.

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: An Interaction of Rhamnolipids with Cu2+ Ions

    doi: 10.3390/molecules23020488

    Figure Lengend Snippet: Dependence of pH and pCu fixed (the negative logarithm of the concentration of Cu 2+ ions fixed by chelating agent) on the Cu 2+ to chelating agent molar ratio (n Cu :n chelating agent ) in water ( a , c , e ) and KCl solution ( b , d , f ) at pH 6.0; ( a , b ) rhamnolipids (Rh); ( c , d ) methylglycinediacetic acid (MGDA); and ( e , f ) ethylenediaminetetraacetic acid (EDTA); CMC Rh means the rhamnolipids’ critical micelle concentration.

    Article Snippet: The Chemicals Used The rhamnolipid R-95 (the 1:1 mixture of mono- and dirhamnolipids with the HPLC purity; the mean molecular weight of 577 g mol−1 , pK a = 5.6), methylglycinediacetic acid (MGDA; analytical grade, pK a = 14.6 [ ]), ethylenediaminetetraacetic acid (EDTA; analytical grade), potassium chloride (KCl), copper (II) sulfate (CuSO4 ; analytical grade), potassium hydroxide (KOH; analytical grade), and sulfuric acid (H2 SO4 ) were purchased from Sigma Aldrich (Poznan, Poland) company.

    Techniques: Concentration Assay

    The structures of ( a ) monorhamnolipid; ( b ) dirhamnolipid; ( c ) ethylenediaminetetraacetic acid (EDTA); and ( d ) methylglycinediacetic acid (MGDA).

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: An Interaction of Rhamnolipids with Cu2+ Ions

    doi: 10.3390/molecules23020488

    Figure Lengend Snippet: The structures of ( a ) monorhamnolipid; ( b ) dirhamnolipid; ( c ) ethylenediaminetetraacetic acid (EDTA); and ( d ) methylglycinediacetic acid (MGDA).

    Article Snippet: The Chemicals Used The rhamnolipid R-95 (the 1:1 mixture of mono- and dirhamnolipids with the HPLC purity; the mean molecular weight of 577 g mol−1 , pK a = 5.6), methylglycinediacetic acid (MGDA; analytical grade, pK a = 14.6 [ ]), ethylenediaminetetraacetic acid (EDTA; analytical grade), potassium chloride (KCl), copper (II) sulfate (CuSO4 ; analytical grade), potassium hydroxide (KOH; analytical grade), and sulfuric acid (H2 SO4 ) were purchased from Sigma Aldrich (Poznan, Poland) company.

    Techniques:

    Schematic of (A) fabrication of the immunosensor and (B) reaction occurring at the working electrode surface in 1 × PBS solution at pH 7.4 containing 1 mM[Fe(CN) 6 ] 3− , 0.1 M KCl, and 100 μM EDTA at scan rate 50 mV s −1 .

    Journal: Biosensors & bioelectronics

    Article Title: An electrochemical immunosensing method for detecting melanoma cells

    doi: 10.1016/j.bios.2015.01.022

    Figure Lengend Snippet: Schematic of (A) fabrication of the immunosensor and (B) reaction occurring at the working electrode surface in 1 × PBS solution at pH 7.4 containing 1 mM[Fe(CN) 6 ] 3− , 0.1 M KCl, and 100 μM EDTA at scan rate 50 mV s −1 .

    Article Snippet: Cells were dislodged from the surface of the culture dish by using 0.25% Trypsin–EDTA (Life Technologies, Grand Island, NY), or 2% EDTA solution in 1 × PBS (Sigma, St. Louis, MO), or a cell scraper (Sarstedt, Newton, NC).

    Techniques:

    (A) Typical cyclic voltammograms of (a) bare SPE (b) PPy/SPE, (c) n-SiNPs/PPy/SPE and (d) MC1R-Ab/n-SiNPs/PPy/SPE in 1 × PBS solution at pH 7.4 containing 1 mM[Fe(CN) 6 ] 3− , 0.1 M KCl, and 100 μM EDTA at scan rate 50 mV s −1

    Journal: Biosensors & bioelectronics

    Article Title: An electrochemical immunosensing method for detecting melanoma cells

    doi: 10.1016/j.bios.2015.01.022

    Figure Lengend Snippet: (A) Typical cyclic voltammograms of (a) bare SPE (b) PPy/SPE, (c) n-SiNPs/PPy/SPE and (d) MC1R-Ab/n-SiNPs/PPy/SPE in 1 × PBS solution at pH 7.4 containing 1 mM[Fe(CN) 6 ] 3− , 0.1 M KCl, and 100 μM EDTA at scan rate 50 mV s −1

    Article Snippet: Cells were dislodged from the surface of the culture dish by using 0.25% Trypsin–EDTA (Life Technologies, Grand Island, NY), or 2% EDTA solution in 1 × PBS (Sigma, St. Louis, MO), or a cell scraper (Sarstedt, Newton, NC).

    Techniques:

    Transport of catechin, EGCG, and their niosomes by human intestinal Caco-2 cells. Notes: Schematic diagram of transcellular transport and different transporters in Caco-2 cells ( A ); effect of concentration on the flux of catechin, EGCG, and their niosomes (0, 10, 20, 50, and 100 μg/mL) through Caco-2 monolayer from apical to basolateral chamber ( B ); effect of temperature (4°C and 37°C), ATP inhibitor (sodium azide), P-gp inhibitor (verapamil), MRP2 inhibitor (MK-571) and absorption enhancer (EDTA) on transport of 100 μg/mL different drugs after 6 hours ( C ). Data are presented as mean ± SD (n=3). Abbreviations: EDTA, ethylenediaminetetraacetic acid; EGCG, (−)-epigallocatechin gallate; MRP2, multidrug resistance-associated protein 2; P-gp, permeability glycoprotein; SD, standard deviation.

    Journal: International Journal of Nanomedicine

    Article Title: Enhanced uptake and transport of (+)-catechin and (-)-epigallocatechin gallate in niosomal formulation by human intestinal Caco-2 cells

    doi: 10.2147/IJN.S59331

    Figure Lengend Snippet: Transport of catechin, EGCG, and their niosomes by human intestinal Caco-2 cells. Notes: Schematic diagram of transcellular transport and different transporters in Caco-2 cells ( A ); effect of concentration on the flux of catechin, EGCG, and their niosomes (0, 10, 20, 50, and 100 μg/mL) through Caco-2 monolayer from apical to basolateral chamber ( B ); effect of temperature (4°C and 37°C), ATP inhibitor (sodium azide), P-gp inhibitor (verapamil), MRP2 inhibitor (MK-571) and absorption enhancer (EDTA) on transport of 100 μg/mL different drugs after 6 hours ( C ). Data are presented as mean ± SD (n=3). Abbreviations: EDTA, ethylenediaminetetraacetic acid; EGCG, (−)-epigallocatechin gallate; MRP2, multidrug resistance-associated protein 2; P-gp, permeability glycoprotein; SD, standard deviation.

    Article Snippet: Chemicals and reagents Catechin, EGCG, sorbitan monostearate (Span 60), CH, dihexadecyl phosphate, fluorescein isothiocyanate (FITC), sulforhodamine B (SRB), fluorescein sodium salt, ascorbic acid (AA), sodium azide, verapamil, 5-(3-(2-(7-chloroquinolin-2-yl)ethenyl)phenyl)-8-dimethylcarbamyl-4,6-dithiaoctanoic acid sodium salt hydrate (MK-571), and ethylenediaminetetraacetic acid (EDTA) were purchased from Sigma-Aldrich (St Louis, MO, USA).

    Techniques: Concentration Assay, Permeability, Standard Deviation

    Impact of different blood anticoagulants on the surface expression of mCD14 on bovine monocytes . Whole blood samples obtained from 18 Holstein cows were anticoagulated with: sodium heparin (HEPARIN), sodium citrate (CITRATE) and ethylenediaminetetraacetic acid (EDTA). The percentage of monocytes expressing mCD14 (2A) and the mean channel fluorescence intensity (MFI) (2B) was measured. Results for each treatment are the mean from 6 cows. Treatment means with different superscripts are significantly different using Scheffe's multiple comparison tests.

    Journal: BMC Research Notes

    Article Title: The influence of different anticoagulants and sample preparation methods on measurement of mCD14 on bovine monocytes and polymorphonuclear neutrophil leukocytes

    doi: 10.1186/1756-0500-5-93

    Figure Lengend Snippet: Impact of different blood anticoagulants on the surface expression of mCD14 on bovine monocytes . Whole blood samples obtained from 18 Holstein cows were anticoagulated with: sodium heparin (HEPARIN), sodium citrate (CITRATE) and ethylenediaminetetraacetic acid (EDTA). The percentage of monocytes expressing mCD14 (2A) and the mean channel fluorescence intensity (MFI) (2B) was measured. Results for each treatment are the mean from 6 cows. Treatment means with different superscripts are significantly different using Scheffe's multiple comparison tests.

    Article Snippet: Blood sampling Blood was collected aseptically from the caudal vein by venipuncture into one of three vacutainer tubes containing either sodium heparin (HEPARIN), sodium citrate (CITRATE) or ethylenediaminetetraacetic acid (EDTA) (Becton Dickinson, Franklin Lakes, NJ, USA).

    Techniques: Expressing, Fluorescence

    Impact of different blood anticoagulants on the surface expression of CD14 on bovine neutrophils . Whole blood samples obtained from 18 Holstein cows were anticoagulated with: sodium heparin (HEPARIN), sodium citrate (CITRATE) and ethylenediaminetetraacetic acid (EDTA). The percentage of PMN expressing mCD14 (1A) and the mean channel fluorescence intensity (MFI) (1B) was measured. Results for each treatment are the mean from 6 cows. Treatment means with different superscripts are significantly different ( p

    Journal: BMC Research Notes

    Article Title: The influence of different anticoagulants and sample preparation methods on measurement of mCD14 on bovine monocytes and polymorphonuclear neutrophil leukocytes

    doi: 10.1186/1756-0500-5-93

    Figure Lengend Snippet: Impact of different blood anticoagulants on the surface expression of CD14 on bovine neutrophils . Whole blood samples obtained from 18 Holstein cows were anticoagulated with: sodium heparin (HEPARIN), sodium citrate (CITRATE) and ethylenediaminetetraacetic acid (EDTA). The percentage of PMN expressing mCD14 (1A) and the mean channel fluorescence intensity (MFI) (1B) was measured. Results for each treatment are the mean from 6 cows. Treatment means with different superscripts are significantly different ( p

    Article Snippet: Blood sampling Blood was collected aseptically from the caudal vein by venipuncture into one of three vacutainer tubes containing either sodium heparin (HEPARIN), sodium citrate (CITRATE) or ethylenediaminetetraacetic acid (EDTA) (Becton Dickinson, Franklin Lakes, NJ, USA).

    Techniques: Expressing, Fluorescence

    Scanning electron micrographs (a) 1% oregano extract solution (OES) + 17% ethylenediaminetetraacetic acid (EDTA) (b) 2% OES + 17% EDTA (c) 5% OES + 17% EDTA

    Journal: European Journal of Dentistry

    Article Title: Antibacterial and smear layer removal capability of oregano extract solution

    doi: 10.4103/1305-7456.149633

    Figure Lengend Snippet: Scanning electron micrographs (a) 1% oregano extract solution (OES) + 17% ethylenediaminetetraacetic acid (EDTA) (b) 2% OES + 17% EDTA (c) 5% OES + 17% EDTA

    Article Snippet: The smear layer was removed in the first seven groups with 3 ml 17% ethylenediaminetetraacetic acid (EDTA) (Merck KGaA, Darmstadt, Germany) for 1-min, followed by 3 ml 5.25% NaOCl (Wizard, Ankara, Turkey) for 1-min, and then 5 ml distillate water for 1-min as outlined by Teixeira et al .

    Techniques:

    Scanning electron micrographs of 5.25 NaOCl + 17% ethylenediaminetetraacetic acid

    Journal: European Journal of Dentistry

    Article Title: Antibacterial and smear layer removal capability of oregano extract solution

    doi: 10.4103/1305-7456.149633

    Figure Lengend Snippet: Scanning electron micrographs of 5.25 NaOCl + 17% ethylenediaminetetraacetic acid

    Article Snippet: The smear layer was removed in the first seven groups with 3 ml 17% ethylenediaminetetraacetic acid (EDTA) (Merck KGaA, Darmstadt, Germany) for 1-min, followed by 3 ml 5.25% NaOCl (Wizard, Ankara, Turkey) for 1-min, and then 5 ml distillate water for 1-min as outlined by Teixeira et al .

    Techniques: