Journal: PLoS ONE
Article Title: TRPM8 Channel Activation Induced by Monoterpenoid Rotundifolone Underlies Mesenteric Artery Relaxation
Figure Lengend Snippet: Rotundifolone-induced Ca 2+ transient in myocytes depend of extracellular Ca 2+ . (A) Representative recording of the Ca 2+ signals induced by rotundifolone (10 −3 and 3x10 -3 M) in freshly dispersed vascular smooth muscle cells from rat superior mesenteric artery. (B) Summary data showing the amplitude of the Ca 2+ signal induced after perfusion with different concentrations of rotundifolone (3x10 -4 , n = 9; 10 −3 , n = 5; 3x10 -3 M, n = 11). (C) Representative recording of Ca 2+ oscillations induced by rotundifolone (3x10 -3 M) in myocytes placed in Ca 2+ -free medium fortified with 2 mM ethylene glycol tetraacetic acid (EGTA). (D) Summary data demonstrating the decreased amplitude of the Ca 2+ signal induced by rotundifolone in Ca 2+ -free medium (n = 13). The Ca 2+ oscillations were measured and represented as increases in fluorescence intensity relative to baseline [DF (%) = (F-F0/F0)*100]. The data were examined using unpaired Student's t tests (rotundifolone with or without EGTA) and one-way ANOVA followed by the Bonferroni post-test. (** p
Article Snippet: Drugs Acetylcholine chloride, L-phenylephrine chloride, ethylene glycol tetraacetic acid (EGTA), capsazepine, ruthenium red, menthol, capsaicin, SKF 96365, BCTC (4-(3-Chloro-2-pyridinyl)-N-[4-(1,1-dimethylethyl)phenyl]-1-piperazinecarboxamide) bovine serum albumin, hyaluronidase type II, chymopapain, DL-dithiothreitol and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (Sigma Chemical Co., St. Louis, MO, USA).