ethylene glycol tetraacetic acid egta Search Results


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  • 99
    Thermo Fisher ethylene glycol tetraacetic acid egta
    Ethylene Glycol Tetraacetic Acid Egta, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore ethylene glycol tetraacetic acid egta
    Rotundifolone-induced Ca 2+ transient in myocytes depend of extracellular Ca 2+ . (A) Representative recording of the Ca 2+ signals induced by rotundifolone (10 −3 and 3x10 -3 M) in freshly dispersed vascular smooth muscle cells from rat superior mesenteric artery. (B) Summary data showing the amplitude of the Ca 2+ signal induced after perfusion with different concentrations of rotundifolone (3x10 -4 , n = 9; 10 −3 , n = 5; 3x10 -3 M, n = 11). (C) Representative recording of Ca 2+ oscillations induced by rotundifolone (3x10 -3 M) in myocytes placed in Ca 2+ -free medium fortified with 2 mM ethylene glycol <t>tetraacetic</t> acid <t>(EGTA).</t> (D) Summary data demonstrating the decreased amplitude of the Ca 2+ signal induced by rotundifolone in Ca 2+ -free medium (n = 13). The Ca 2+ oscillations were measured and represented as increases in fluorescence intensity relative to baseline [DF (%) = (F-F0/F0)*100]. The data were examined using unpaired Student's t tests (rotundifolone with or without EGTA) and one-way ANOVA followed by the Bonferroni post-test. (** p
    Ethylene Glycol Tetraacetic Acid Egta, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 796 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Tocris ethylene glycol tetraacetic acid egta
    Rotundifolone-induced Ca 2+ transient in myocytes depend of extracellular Ca 2+ . (A) Representative recording of the Ca 2+ signals induced by rotundifolone (10 −3 and 3x10 -3 M) in freshly dispersed vascular smooth muscle cells from rat superior mesenteric artery. (B) Summary data showing the amplitude of the Ca 2+ signal induced after perfusion with different concentrations of rotundifolone (3x10 -4 , n = 9; 10 −3 , n = 5; 3x10 -3 M, n = 11). (C) Representative recording of Ca 2+ oscillations induced by rotundifolone (3x10 -3 M) in myocytes placed in Ca 2+ -free medium fortified with 2 mM ethylene glycol <t>tetraacetic</t> acid <t>(EGTA).</t> (D) Summary data demonstrating the decreased amplitude of the Ca 2+ signal induced by rotundifolone in Ca 2+ -free medium (n = 13). The Ca 2+ oscillations were measured and represented as increases in fluorescence intensity relative to baseline [DF (%) = (F-F0/F0)*100]. The data were examined using unpaired Student's t tests (rotundifolone with or without EGTA) and one-way ANOVA followed by the Bonferroni post-test. (** p
    Ethylene Glycol Tetraacetic Acid Egta, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Amresco ethylene glycol tetraacetic acid egta
    Rotundifolone-induced Ca 2+ transient in myocytes depend of extracellular Ca 2+ . (A) Representative recording of the Ca 2+ signals induced by rotundifolone (10 −3 and 3x10 -3 M) in freshly dispersed vascular smooth muscle cells from rat superior mesenteric artery. (B) Summary data showing the amplitude of the Ca 2+ signal induced after perfusion with different concentrations of rotundifolone (3x10 -4 , n = 9; 10 −3 , n = 5; 3x10 -3 M, n = 11). (C) Representative recording of Ca 2+ oscillations induced by rotundifolone (3x10 -3 M) in myocytes placed in Ca 2+ -free medium fortified with 2 mM ethylene glycol <t>tetraacetic</t> acid <t>(EGTA).</t> (D) Summary data demonstrating the decreased amplitude of the Ca 2+ signal induced by rotundifolone in Ca 2+ -free medium (n = 13). The Ca 2+ oscillations were measured and represented as increases in fluorescence intensity relative to baseline [DF (%) = (F-F0/F0)*100]. The data were examined using unpaired Student's t tests (rotundifolone with or without EGTA) and one-way ANOVA followed by the Bonferroni post-test. (** p
    Ethylene Glycol Tetraacetic Acid Egta, supplied by Amresco, used in various techniques. Bioz Stars score: 93/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioShop ethylene glycol tetraacetic acid egta
    Rotundifolone-induced Ca 2+ transient in myocytes depend of extracellular Ca 2+ . (A) Representative recording of the Ca 2+ signals induced by rotundifolone (10 −3 and 3x10 -3 M) in freshly dispersed vascular smooth muscle cells from rat superior mesenteric artery. (B) Summary data showing the amplitude of the Ca 2+ signal induced after perfusion with different concentrations of rotundifolone (3x10 -4 , n = 9; 10 −3 , n = 5; 3x10 -3 M, n = 11). (C) Representative recording of Ca 2+ oscillations induced by rotundifolone (3x10 -3 M) in myocytes placed in Ca 2+ -free medium fortified with 2 mM ethylene glycol <t>tetraacetic</t> acid <t>(EGTA).</t> (D) Summary data demonstrating the decreased amplitude of the Ca 2+ signal induced by rotundifolone in Ca 2+ -free medium (n = 13). The Ca 2+ oscillations were measured and represented as increases in fluorescence intensity relative to baseline [DF (%) = (F-F0/F0)*100]. The data were examined using unpaired Student's t tests (rotundifolone with or without EGTA) and one-way ANOVA followed by the Bonferroni post-test. (** p
    Ethylene Glycol Tetraacetic Acid Egta, supplied by BioShop, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Dojindo Labs ethylene glycol tetraacetic acid egta
    Rotundifolone-induced Ca 2+ transient in myocytes depend of extracellular Ca 2+ . (A) Representative recording of the Ca 2+ signals induced by rotundifolone (10 −3 and 3x10 -3 M) in freshly dispersed vascular smooth muscle cells from rat superior mesenteric artery. (B) Summary data showing the amplitude of the Ca 2+ signal induced after perfusion with different concentrations of rotundifolone (3x10 -4 , n = 9; 10 −3 , n = 5; 3x10 -3 M, n = 11). (C) Representative recording of Ca 2+ oscillations induced by rotundifolone (3x10 -3 M) in myocytes placed in Ca 2+ -free medium fortified with 2 mM ethylene glycol <t>tetraacetic</t> acid <t>(EGTA).</t> (D) Summary data demonstrating the decreased amplitude of the Ca 2+ signal induced by rotundifolone in Ca 2+ -free medium (n = 13). The Ca 2+ oscillations were measured and represented as increases in fluorescence intensity relative to baseline [DF (%) = (F-F0/F0)*100]. The data were examined using unpaired Student's t tests (rotundifolone with or without EGTA) and one-way ANOVA followed by the Bonferroni post-test. (** p
    Ethylene Glycol Tetraacetic Acid Egta, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GERBU Biotechnik GmbH ethylene glycol tetraacetic acid egta
    Rotundifolone-induced Ca 2+ transient in myocytes depend of extracellular Ca 2+ . (A) Representative recording of the Ca 2+ signals induced by rotundifolone (10 −3 and 3x10 -3 M) in freshly dispersed vascular smooth muscle cells from rat superior mesenteric artery. (B) Summary data showing the amplitude of the Ca 2+ signal induced after perfusion with different concentrations of rotundifolone (3x10 -4 , n = 9; 10 −3 , n = 5; 3x10 -3 M, n = 11). (C) Representative recording of Ca 2+ oscillations induced by rotundifolone (3x10 -3 M) in myocytes placed in Ca 2+ -free medium fortified with 2 mM ethylene glycol <t>tetraacetic</t> acid <t>(EGTA).</t> (D) Summary data demonstrating the decreased amplitude of the Ca 2+ signal induced by rotundifolone in Ca 2+ -free medium (n = 13). The Ca 2+ oscillations were measured and represented as increases in fluorescence intensity relative to baseline [DF (%) = (F-F0/F0)*100]. The data were examined using unpaired Student's t tests (rotundifolone with or without EGTA) and one-way ANOVA followed by the Bonferroni post-test. (** p
    Ethylene Glycol Tetraacetic Acid Egta, supplied by GERBU Biotechnik GmbH, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co ethylene glycol tetraacetic acid egta
    Rotundifolone-induced Ca 2+ transient in myocytes depend of extracellular Ca 2+ . (A) Representative recording of the Ca 2+ signals induced by rotundifolone (10 −3 and 3x10 -3 M) in freshly dispersed vascular smooth muscle cells from rat superior mesenteric artery. (B) Summary data showing the amplitude of the Ca 2+ signal induced after perfusion with different concentrations of rotundifolone (3x10 -4 , n = 9; 10 −3 , n = 5; 3x10 -3 M, n = 11). (C) Representative recording of Ca 2+ oscillations induced by rotundifolone (3x10 -3 M) in myocytes placed in Ca 2+ -free medium fortified with 2 mM ethylene glycol <t>tetraacetic</t> acid <t>(EGTA).</t> (D) Summary data demonstrating the decreased amplitude of the Ca 2+ signal induced by rotundifolone in Ca 2+ -free medium (n = 13). The Ca 2+ oscillations were measured and represented as increases in fluorescence intensity relative to baseline [DF (%) = (F-F0/F0)*100]. The data were examined using unpaired Student's t tests (rotundifolone with or without EGTA) and one-way ANOVA followed by the Bonferroni post-test. (** p
    Ethylene Glycol Tetraacetic Acid Egta, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fisher Scientific ethylene glycol tetraacetic acid egta treated
    Rotundifolone-induced Ca 2+ transient in myocytes depend of extracellular Ca 2+ . (A) Representative recording of the Ca 2+ signals induced by rotundifolone (10 −3 and 3x10 -3 M) in freshly dispersed vascular smooth muscle cells from rat superior mesenteric artery. (B) Summary data showing the amplitude of the Ca 2+ signal induced after perfusion with different concentrations of rotundifolone (3x10 -4 , n = 9; 10 −3 , n = 5; 3x10 -3 M, n = 11). (C) Representative recording of Ca 2+ oscillations induced by rotundifolone (3x10 -3 M) in myocytes placed in Ca 2+ -free medium fortified with 2 mM ethylene glycol <t>tetraacetic</t> acid <t>(EGTA).</t> (D) Summary data demonstrating the decreased amplitude of the Ca 2+ signal induced by rotundifolone in Ca 2+ -free medium (n = 13). The Ca 2+ oscillations were measured and represented as increases in fluorescence intensity relative to baseline [DF (%) = (F-F0/F0)*100]. The data were examined using unpaired Student's t tests (rotundifolone with or without EGTA) and one-way ANOVA followed by the Bonferroni post-test. (** p
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    Roche ethylene glycol tetraacetic acid egta
    Rotundifolone-induced Ca 2+ transient in myocytes depend of extracellular Ca 2+ . (A) Representative recording of the Ca 2+ signals induced by rotundifolone (10 −3 and 3x10 -3 M) in freshly dispersed vascular smooth muscle cells from rat superior mesenteric artery. (B) Summary data showing the amplitude of the Ca 2+ signal induced after perfusion with different concentrations of rotundifolone (3x10 -4 , n = 9; 10 −3 , n = 5; 3x10 -3 M, n = 11). (C) Representative recording of Ca 2+ oscillations induced by rotundifolone (3x10 -3 M) in myocytes placed in Ca 2+ -free medium fortified with 2 mM ethylene glycol <t>tetraacetic</t> acid <t>(EGTA).</t> (D) Summary data demonstrating the decreased amplitude of the Ca 2+ signal induced by rotundifolone in Ca 2+ -free medium (n = 13). The Ca 2+ oscillations were measured and represented as increases in fluorescence intensity relative to baseline [DF (%) = (F-F0/F0)*100]. The data were examined using unpaired Student's t tests (rotundifolone with or without EGTA) and one-way ANOVA followed by the Bonferroni post-test. (** p
    Ethylene Glycol Tetraacetic Acid Egta, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 477 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    FUJIFILM ethylene glycol tetraacetic acid egta
    Rotundifolone-induced Ca 2+ transient in myocytes depend of extracellular Ca 2+ . (A) Representative recording of the Ca 2+ signals induced by rotundifolone (10 −3 and 3x10 -3 M) in freshly dispersed vascular smooth muscle cells from rat superior mesenteric artery. (B) Summary data showing the amplitude of the Ca 2+ signal induced after perfusion with different concentrations of rotundifolone (3x10 -4 , n = 9; 10 −3 , n = 5; 3x10 -3 M, n = 11). (C) Representative recording of Ca 2+ oscillations induced by rotundifolone (3x10 -3 M) in myocytes placed in Ca 2+ -free medium fortified with 2 mM ethylene glycol <t>tetraacetic</t> acid <t>(EGTA).</t> (D) Summary data demonstrating the decreased amplitude of the Ca 2+ signal induced by rotundifolone in Ca 2+ -free medium (n = 13). The Ca 2+ oscillations were measured and represented as increases in fluorescence intensity relative to baseline [DF (%) = (F-F0/F0)*100]. The data were examined using unpaired Student's t tests (rotundifolone with or without EGTA) and one-way ANOVA followed by the Bonferroni post-test. (** p
    Ethylene Glycol Tetraacetic Acid Egta, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Avantor ethylene glycol tetraacetic acid egta
    Rotundifolone-induced Ca 2+ transient in myocytes depend of extracellular Ca 2+ . (A) Representative recording of the Ca 2+ signals induced by rotundifolone (10 −3 and 3x10 -3 M) in freshly dispersed vascular smooth muscle cells from rat superior mesenteric artery. (B) Summary data showing the amplitude of the Ca 2+ signal induced after perfusion with different concentrations of rotundifolone (3x10 -4 , n = 9; 10 −3 , n = 5; 3x10 -3 M, n = 11). (C) Representative recording of Ca 2+ oscillations induced by rotundifolone (3x10 -3 M) in myocytes placed in Ca 2+ -free medium fortified with 2 mM ethylene glycol <t>tetraacetic</t> acid <t>(EGTA).</t> (D) Summary data demonstrating the decreased amplitude of the Ca 2+ signal induced by rotundifolone in Ca 2+ -free medium (n = 13). The Ca 2+ oscillations were measured and represented as increases in fluorescence intensity relative to baseline [DF (%) = (F-F0/F0)*100]. The data were examined using unpaired Student's t tests (rotundifolone with or without EGTA) and one-way ANOVA followed by the Bonferroni post-test. (** p
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    GE Healthcare ethylene glycol tetraacetic acid egta
    Rotundifolone-induced Ca 2+ transient in myocytes depend of extracellular Ca 2+ . (A) Representative recording of the Ca 2+ signals induced by rotundifolone (10 −3 and 3x10 -3 M) in freshly dispersed vascular smooth muscle cells from rat superior mesenteric artery. (B) Summary data showing the amplitude of the Ca 2+ signal induced after perfusion with different concentrations of rotundifolone (3x10 -4 , n = 9; 10 −3 , n = 5; 3x10 -3 M, n = 11). (C) Representative recording of Ca 2+ oscillations induced by rotundifolone (3x10 -3 M) in myocytes placed in Ca 2+ -free medium fortified with 2 mM ethylene glycol <t>tetraacetic</t> acid <t>(EGTA).</t> (D) Summary data demonstrating the decreased amplitude of the Ca 2+ signal induced by rotundifolone in Ca 2+ -free medium (n = 13). The Ca 2+ oscillations were measured and represented as increases in fluorescence intensity relative to baseline [DF (%) = (F-F0/F0)*100]. The data were examined using unpaired Student's t tests (rotundifolone with or without EGTA) and one-way ANOVA followed by the Bonferroni post-test. (** p
    Ethylene Glycol Tetraacetic Acid Egta, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA ethylene glycol tetraacetic acid egta
    Rotundifolone-induced Ca 2+ transient in myocytes depend of extracellular Ca 2+ . (A) Representative recording of the Ca 2+ signals induced by rotundifolone (10 −3 and 3x10 -3 M) in freshly dispersed vascular smooth muscle cells from rat superior mesenteric artery. (B) Summary data showing the amplitude of the Ca 2+ signal induced after perfusion with different concentrations of rotundifolone (3x10 -4 , n = 9; 10 −3 , n = 5; 3x10 -3 M, n = 11). (C) Representative recording of Ca 2+ oscillations induced by rotundifolone (3x10 -3 M) in myocytes placed in Ca 2+ -free medium fortified with 2 mM ethylene glycol <t>tetraacetic</t> acid <t>(EGTA).</t> (D) Summary data demonstrating the decreased amplitude of the Ca 2+ signal induced by rotundifolone in Ca 2+ -free medium (n = 13). The Ca 2+ oscillations were measured and represented as increases in fluorescence intensity relative to baseline [DF (%) = (F-F0/F0)*100]. The data were examined using unpaired Student's t tests (rotundifolone with or without EGTA) and one-way ANOVA followed by the Bonferroni post-test. (** p
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    Bio-Rad ethylene glycol tetraacetic acid egta
    Rotundifolone-induced Ca 2+ transient in myocytes depend of extracellular Ca 2+ . (A) Representative recording of the Ca 2+ signals induced by rotundifolone (10 −3 and 3x10 -3 M) in freshly dispersed vascular smooth muscle cells from rat superior mesenteric artery. (B) Summary data showing the amplitude of the Ca 2+ signal induced after perfusion with different concentrations of rotundifolone (3x10 -4 , n = 9; 10 −3 , n = 5; 3x10 -3 M, n = 11). (C) Representative recording of Ca 2+ oscillations induced by rotundifolone (3x10 -3 M) in myocytes placed in Ca 2+ -free medium fortified with 2 mM ethylene glycol <t>tetraacetic</t> acid <t>(EGTA).</t> (D) Summary data demonstrating the decreased amplitude of the Ca 2+ signal induced by rotundifolone in Ca 2+ -free medium (n = 13). The Ca 2+ oscillations were measured and represented as increases in fluorescence intensity relative to baseline [DF (%) = (F-F0/F0)*100]. The data were examined using unpaired Student's t tests (rotundifolone with or without EGTA) and one-way ANOVA followed by the Bonferroni post-test. (** p
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    Rotundifolone-induced Ca 2+ transient in myocytes depend of extracellular Ca 2+ . (A) Representative recording of the Ca 2+ signals induced by rotundifolone (10 −3 and 3x10 -3 M) in freshly dispersed vascular smooth muscle cells from rat superior mesenteric artery. (B) Summary data showing the amplitude of the Ca 2+ signal induced after perfusion with different concentrations of rotundifolone (3x10 -4 , n = 9; 10 −3 , n = 5; 3x10 -3 M, n = 11). (C) Representative recording of Ca 2+ oscillations induced by rotundifolone (3x10 -3 M) in myocytes placed in Ca 2+ -free medium fortified with 2 mM ethylene glycol <t>tetraacetic</t> acid <t>(EGTA).</t> (D) Summary data demonstrating the decreased amplitude of the Ca 2+ signal induced by rotundifolone in Ca 2+ -free medium (n = 13). The Ca 2+ oscillations were measured and represented as increases in fluorescence intensity relative to baseline [DF (%) = (F-F0/F0)*100]. The data were examined using unpaired Student's t tests (rotundifolone with or without EGTA) and one-way ANOVA followed by the Bonferroni post-test. (** p
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    Rotundifolone-induced Ca 2+ transient in myocytes depend of extracellular Ca 2+ . (A) Representative recording of the Ca 2+ signals induced by rotundifolone (10 −3 and 3x10 -3 M) in freshly dispersed vascular smooth muscle cells from rat superior mesenteric artery. (B) Summary data showing the amplitude of the Ca 2+ signal induced after perfusion with different concentrations of rotundifolone (3x10 -4 , n = 9; 10 −3 , n = 5; 3x10 -3 M, n = 11). (C) Representative recording of Ca 2+ oscillations induced by rotundifolone (3x10 -3 M) in myocytes placed in Ca 2+ -free medium fortified with 2 mM ethylene glycol <t>tetraacetic</t> acid <t>(EGTA).</t> (D) Summary data demonstrating the decreased amplitude of the Ca 2+ signal induced by rotundifolone in Ca 2+ -free medium (n = 13). The Ca 2+ oscillations were measured and represented as increases in fluorescence intensity relative to baseline [DF (%) = (F-F0/F0)*100]. The data were examined using unpaired Student's t tests (rotundifolone with or without EGTA) and one-way ANOVA followed by the Bonferroni post-test. (** p
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    Rotundifolone-induced Ca 2+ transient in myocytes depend of extracellular Ca 2+ . (A) Representative recording of the Ca 2+ signals induced by rotundifolone (10 −3 and 3x10 -3 M) in freshly dispersed vascular smooth muscle cells from rat superior mesenteric artery. (B) Summary data showing the amplitude of the Ca 2+ signal induced after perfusion with different concentrations of rotundifolone (3x10 -4 , n = 9; 10 −3 , n = 5; 3x10 -3 M, n = 11). (C) Representative recording of Ca 2+ oscillations induced by rotundifolone (3x10 -3 M) in myocytes placed in Ca 2+ -free medium fortified with 2 mM ethylene glycol <t>tetraacetic</t> acid <t>(EGTA).</t> (D) Summary data demonstrating the decreased amplitude of the Ca 2+ signal induced by rotundifolone in Ca 2+ -free medium (n = 13). The Ca 2+ oscillations were measured and represented as increases in fluorescence intensity relative to baseline [DF (%) = (F-F0/F0)*100]. The data were examined using unpaired Student's t tests (rotundifolone with or without EGTA) and one-way ANOVA followed by the Bonferroni post-test. (** p
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    Rotundifolone-induced Ca 2+ transient in myocytes depend of extracellular Ca 2+ . (A) Representative recording of the Ca 2+ signals induced by rotundifolone (10 −3 and 3x10 -3 M) in freshly dispersed vascular smooth muscle cells from rat superior mesenteric artery. (B) Summary data showing the amplitude of the Ca 2+ signal induced after perfusion with different concentrations of rotundifolone (3x10 -4 , n = 9; 10 −3 , n = 5; 3x10 -3 M, n = 11). (C) Representative recording of Ca 2+ oscillations induced by rotundifolone (3x10 -3 M) in myocytes placed in Ca 2+ -free medium fortified with 2 mM ethylene glycol <t>tetraacetic</t> acid <t>(EGTA).</t> (D) Summary data demonstrating the decreased amplitude of the Ca 2+ signal induced by rotundifolone in Ca 2+ -free medium (n = 13). The Ca 2+ oscillations were measured and represented as increases in fluorescence intensity relative to baseline [DF (%) = (F-F0/F0)*100]. The data were examined using unpaired Student's t tests (rotundifolone with or without EGTA) and one-way ANOVA followed by the Bonferroni post-test. (** p
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    Rotundifolone-induced Ca 2+ transient in myocytes depend of extracellular Ca 2+ . (A) Representative recording of the Ca 2+ signals induced by rotundifolone (10 −3 and 3x10 -3 M) in freshly dispersed vascular smooth muscle cells from rat superior mesenteric artery. (B) Summary data showing the amplitude of the Ca 2+ signal induced after perfusion with different concentrations of rotundifolone (3x10 -4 , n = 9; 10 −3 , n = 5; 3x10 -3 M, n = 11). (C) Representative recording of Ca 2+ oscillations induced by rotundifolone (3x10 -3 M) in myocytes placed in Ca 2+ -free medium fortified with 2 mM ethylene glycol tetraacetic acid (EGTA). (D) Summary data demonstrating the decreased amplitude of the Ca 2+ signal induced by rotundifolone in Ca 2+ -free medium (n = 13). The Ca 2+ oscillations were measured and represented as increases in fluorescence intensity relative to baseline [DF (%) = (F-F0/F0)*100]. The data were examined using unpaired Student's t tests (rotundifolone with or without EGTA) and one-way ANOVA followed by the Bonferroni post-test. (** p

    Journal: PLoS ONE

    Article Title: TRPM8 Channel Activation Induced by Monoterpenoid Rotundifolone Underlies Mesenteric Artery Relaxation

    doi: 10.1371/journal.pone.0143171

    Figure Lengend Snippet: Rotundifolone-induced Ca 2+ transient in myocytes depend of extracellular Ca 2+ . (A) Representative recording of the Ca 2+ signals induced by rotundifolone (10 −3 and 3x10 -3 M) in freshly dispersed vascular smooth muscle cells from rat superior mesenteric artery. (B) Summary data showing the amplitude of the Ca 2+ signal induced after perfusion with different concentrations of rotundifolone (3x10 -4 , n = 9; 10 −3 , n = 5; 3x10 -3 M, n = 11). (C) Representative recording of Ca 2+ oscillations induced by rotundifolone (3x10 -3 M) in myocytes placed in Ca 2+ -free medium fortified with 2 mM ethylene glycol tetraacetic acid (EGTA). (D) Summary data demonstrating the decreased amplitude of the Ca 2+ signal induced by rotundifolone in Ca 2+ -free medium (n = 13). The Ca 2+ oscillations were measured and represented as increases in fluorescence intensity relative to baseline [DF (%) = (F-F0/F0)*100]. The data were examined using unpaired Student's t tests (rotundifolone with or without EGTA) and one-way ANOVA followed by the Bonferroni post-test. (** p

    Article Snippet: Drugs Acetylcholine chloride, L-phenylephrine chloride, ethylene glycol tetraacetic acid (EGTA), capsazepine, ruthenium red, menthol, capsaicin, SKF 96365, BCTC (4-(3-Chloro-2-pyridinyl)-N-[4-(1,1-dimethylethyl)phenyl]-1-piperazinecarboxamide) bovine serum albumin, hyaluronidase type II, chymopapain, DL-dithiothreitol and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (Sigma Chemical Co., St. Louis, MO, USA).

    Techniques: Fluorescence