ethyl heptanoate Search Results


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  • 92
    Millipore ethyl heptanoate
    Medium-chain fatty acids and ketones mitigate TDP-43 dependent locomotor dysfunction in a Drosophila model of ALS. (A,B) Larval turning times, used to measure locomotor deficits caused by TDP WT or TDP G298S , are rescued by feeding different medium-chain fatty acids [coconut oil (A) or ethyl <t>heptanoate</t> (B) ]. (C) Ketone (beta-hydroxybutyrate) feeding reduces larval turning times in ALS larvae. TDP WT or TDP G298S were expressed in motor neurons using D42 GAL4. Genotypes and treatments as indicated. Kruskal-Wallis multiple comparisons test was used to calculate significance. * P value
    Ethyl Heptanoate, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 1273 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore heptane ethyl acetate
    Medium-chain fatty acids and ketones mitigate TDP-43 dependent locomotor dysfunction in a Drosophila model of ALS. (A,B) Larval turning times, used to measure locomotor deficits caused by TDP WT or TDP G298S , are rescued by feeding different medium-chain fatty acids [coconut oil (A) or ethyl <t>heptanoate</t> (B) ]. (C) Ketone (beta-hydroxybutyrate) feeding reduces larval turning times in ALS larvae. TDP WT or TDP G298S were expressed in motor neurons using D42 GAL4. Genotypes and treatments as indicated. Kruskal-Wallis multiple comparisons test was used to calculate significance. * P value
    Heptane Ethyl Acetate, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore n hexane ethyl acetate
    SMSr forms SAM-dependent homo-oligomers in the ER. ( a ) Detergent extracts of HeLa cells co-transfected with HA-tagged SMSr and V5/His6-tagged SMSr, SMSr∆SAM, SMSr-KKSA or SMSr∆SAM-KKSA were subjected to immunoprecipitation analysis using an anti-V5 antibody. Immunoprecipitates (IP) and total extracts (input) were immunoblotted (IB) using anti-V5 and anti-HA antibodies. An asterisk denotes immunoreactivity with IgG heavy chain. ( b ) Detergent extracts of HeLa cells co-transfected with HA-tagged SMS1 and V5/His6-tagged SMS1 or SMS1∆SAM were subjected to immunoprecipitation analysis using an anti-V5 antibody. Immunoprecipitates (IP) and total extracts (input) were immunoblotted (IB) using anti-V5 and anti-HA antibodies, as in ( a ). ( c ) HeLa cells transfected with empty vector (EV) or V5/His6-tagged SMSr were solubilized using detergent in the presence or absence of 10 mM N -ethyl <t>maleimide</t> <t>(NEM)</t> and subjected to Ni 2+ -NTA affinity chromatography. Ni 2+ -NTA eluates were incubated in the presence or absence of 100 mM DTT, and immunoblotted (IB) using an anti-V5 antibody, as in ( a ). ( d ) HeLa cells transfected with HA-tagged SMSr were treated with chemical crosslinker DSP (0–50 μM, 15 min, RT), solubilized by detergent in the presence of 10 mM NEM and subjected to immunoprecipitation analysis using an anti-HA antibody. Immunoprecipitates were immunoblotted using an anti-HA antibody. ( e ) Yeast cells transfected with empty vector (EV) or V5/His6-tagged SMSr were treated with DSP as in ( d ), lysed and subjected to immunoblot analysis using an anti-V5 antibody. ( f ) HeLa cells transfected with empty vector (EV), V5/His6-tagged SMSr or SMSr∆SAM were incubated in the presence or absence of DSP (50 μM, 15 min, RT), solubilized with detergent in the presence of 10 mM NEM and subjected to Ni 2+ -NTA affinity chromatography. Ni 2+ -NTA eluates were immunoblotted using an anti-V5 antibody. Uncropped images of blots are provided in Supplementary Fig. S1 .
    N Hexane Ethyl Acetate, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore protease inhibitors chymostatin 1 chloro 3 tosylamido 7 amino 2 heptanone l tosylamido 2 phenyl ethyl chloromethyl ketone
    SMSr forms SAM-dependent homo-oligomers in the ER. ( a ) Detergent extracts of HeLa cells co-transfected with HA-tagged SMSr and V5/His6-tagged SMSr, SMSr∆SAM, SMSr-KKSA or SMSr∆SAM-KKSA were subjected to immunoprecipitation analysis using an anti-V5 antibody. Immunoprecipitates (IP) and total extracts (input) were immunoblotted (IB) using anti-V5 and anti-HA antibodies. An asterisk denotes immunoreactivity with IgG heavy chain. ( b ) Detergent extracts of HeLa cells co-transfected with HA-tagged SMS1 and V5/His6-tagged SMS1 or SMS1∆SAM were subjected to immunoprecipitation analysis using an anti-V5 antibody. Immunoprecipitates (IP) and total extracts (input) were immunoblotted (IB) using anti-V5 and anti-HA antibodies, as in ( a ). ( c ) HeLa cells transfected with empty vector (EV) or V5/His6-tagged SMSr were solubilized using detergent in the presence or absence of 10 mM N -ethyl <t>maleimide</t> <t>(NEM)</t> and subjected to Ni 2+ -NTA affinity chromatography. Ni 2+ -NTA eluates were incubated in the presence or absence of 100 mM DTT, and immunoblotted (IB) using an anti-V5 antibody, as in ( a ). ( d ) HeLa cells transfected with HA-tagged SMSr were treated with chemical crosslinker DSP (0–50 μM, 15 min, RT), solubilized by detergent in the presence of 10 mM NEM and subjected to immunoprecipitation analysis using an anti-HA antibody. Immunoprecipitates were immunoblotted using an anti-HA antibody. ( e ) Yeast cells transfected with empty vector (EV) or V5/His6-tagged SMSr were treated with DSP as in ( d ), lysed and subjected to immunoblot analysis using an anti-V5 antibody. ( f ) HeLa cells transfected with empty vector (EV), V5/His6-tagged SMSr or SMSr∆SAM were incubated in the presence or absence of DSP (50 μM, 15 min, RT), solubilized with detergent in the presence of 10 mM NEM and subjected to Ni 2+ -NTA affinity chromatography. Ni 2+ -NTA eluates were immunoblotted using an anti-V5 antibody. Uncropped images of blots are provided in Supplementary Fig. S1 .
    Protease Inhibitors Chymostatin 1 Chloro 3 Tosylamido 7 Amino 2 Heptanone L Tosylamido 2 Phenyl Ethyl Chloromethyl Ketone, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    protease inhibitors chymostatin 1 chloro 3 tosylamido 7 amino 2 heptanone l tosylamido 2 phenyl ethyl chloromethyl ketone - by Bioz Stars, 2020-10
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    Image Search Results


    Medium-chain fatty acids and ketones mitigate TDP-43 dependent locomotor dysfunction in a Drosophila model of ALS. (A,B) Larval turning times, used to measure locomotor deficits caused by TDP WT or TDP G298S , are rescued by feeding different medium-chain fatty acids [coconut oil (A) or ethyl heptanoate (B) ]. (C) Ketone (beta-hydroxybutyrate) feeding reduces larval turning times in ALS larvae. TDP WT or TDP G298S were expressed in motor neurons using D42 GAL4. Genotypes and treatments as indicated. Kruskal-Wallis multiple comparisons test was used to calculate significance. * P value

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Medium-Chain Fatty Acids, Beta-Hydroxybutyric Acid and Genetic Modulation of the Carnitine Shuttle Are Protective in a Drosophila Model of ALS Based on TDP-43

    doi: 10.3389/fnmol.2018.00182

    Figure Lengend Snippet: Medium-chain fatty acids and ketones mitigate TDP-43 dependent locomotor dysfunction in a Drosophila model of ALS. (A,B) Larval turning times, used to measure locomotor deficits caused by TDP WT or TDP G298S , are rescued by feeding different medium-chain fatty acids [coconut oil (A) or ethyl heptanoate (B) ]. (C) Ketone (beta-hydroxybutyrate) feeding reduces larval turning times in ALS larvae. TDP WT or TDP G298S were expressed in motor neurons using D42 GAL4. Genotypes and treatments as indicated. Kruskal-Wallis multiple comparisons test was used to calculate significance. * P value

    Article Snippet: The food was supplemented with either coconut oil [organic, expeller pressed unrefined virgin coconut oil (365 everyday value, Whole Foods market)], ethyl heptanoate (Sigma cat#: W243728) or sodium R-,S-beta-hydroxybutyrate (BHB, Sigma cat#: H6501).

    Techniques:

    Scatterplots and boxplots of active (red) and passive (blue) RV diastolic pressure components measured at the times of pulmonary valve closure, tricuspid valve opening, minimal RV pressure, and end-diastole at baseline ( n = 13), esmolol ( n = 12), dobutamine

    Journal: Cardiovascular Research

    Article Title: The role of elastic restoring forces in right-ventricular filling

    doi: 10.1093/cvr/cvv047

    Figure Lengend Snippet: Scatterplots and boxplots of active (red) and passive (blue) RV diastolic pressure components measured at the times of pulmonary valve closure, tricuspid valve opening, minimal RV pressure, and end-diastole at baseline ( n = 13), esmolol ( n = 12), dobutamine

    Article Snippet: We performed a sequential data acquisition protocol which included baseline ( n = 13), esmolol (75–200 µg/kg/min; n = 12), and dobutamine (2.5 µg/kg/min; n = 11) infusions, acute volume overload (1000–1500 mL saline isotonic solution in 5–10 min; n = 11), and endotoxic RV failure [lipopolysaccharide from Escherichia coli serotype 0127:B8 (Sigma Chemical); 0.5 mg/kg over 30 min infusion].

    Techniques:

    SMSr forms SAM-dependent homo-oligomers in the ER. ( a ) Detergent extracts of HeLa cells co-transfected with HA-tagged SMSr and V5/His6-tagged SMSr, SMSr∆SAM, SMSr-KKSA or SMSr∆SAM-KKSA were subjected to immunoprecipitation analysis using an anti-V5 antibody. Immunoprecipitates (IP) and total extracts (input) were immunoblotted (IB) using anti-V5 and anti-HA antibodies. An asterisk denotes immunoreactivity with IgG heavy chain. ( b ) Detergent extracts of HeLa cells co-transfected with HA-tagged SMS1 and V5/His6-tagged SMS1 or SMS1∆SAM were subjected to immunoprecipitation analysis using an anti-V5 antibody. Immunoprecipitates (IP) and total extracts (input) were immunoblotted (IB) using anti-V5 and anti-HA antibodies, as in ( a ). ( c ) HeLa cells transfected with empty vector (EV) or V5/His6-tagged SMSr were solubilized using detergent in the presence or absence of 10 mM N -ethyl maleimide (NEM) and subjected to Ni 2+ -NTA affinity chromatography. Ni 2+ -NTA eluates were incubated in the presence or absence of 100 mM DTT, and immunoblotted (IB) using an anti-V5 antibody, as in ( a ). ( d ) HeLa cells transfected with HA-tagged SMSr were treated with chemical crosslinker DSP (0–50 μM, 15 min, RT), solubilized by detergent in the presence of 10 mM NEM and subjected to immunoprecipitation analysis using an anti-HA antibody. Immunoprecipitates were immunoblotted using an anti-HA antibody. ( e ) Yeast cells transfected with empty vector (EV) or V5/His6-tagged SMSr were treated with DSP as in ( d ), lysed and subjected to immunoblot analysis using an anti-V5 antibody. ( f ) HeLa cells transfected with empty vector (EV), V5/His6-tagged SMSr or SMSr∆SAM were incubated in the presence or absence of DSP (50 μM, 15 min, RT), solubilized with detergent in the presence of 10 mM NEM and subjected to Ni 2+ -NTA affinity chromatography. Ni 2+ -NTA eluates were immunoblotted using an anti-V5 antibody. Uncropped images of blots are provided in Supplementary Fig. S1 .

    Journal: Scientific Reports

    Article Title: ER residency of the ceramide phosphoethanolamine synthase SMSr relies on homotypic oligomerization mediated by its SAM domain

    doi: 10.1038/srep41290

    Figure Lengend Snippet: SMSr forms SAM-dependent homo-oligomers in the ER. ( a ) Detergent extracts of HeLa cells co-transfected with HA-tagged SMSr and V5/His6-tagged SMSr, SMSr∆SAM, SMSr-KKSA or SMSr∆SAM-KKSA were subjected to immunoprecipitation analysis using an anti-V5 antibody. Immunoprecipitates (IP) and total extracts (input) were immunoblotted (IB) using anti-V5 and anti-HA antibodies. An asterisk denotes immunoreactivity with IgG heavy chain. ( b ) Detergent extracts of HeLa cells co-transfected with HA-tagged SMS1 and V5/His6-tagged SMS1 or SMS1∆SAM were subjected to immunoprecipitation analysis using an anti-V5 antibody. Immunoprecipitates (IP) and total extracts (input) were immunoblotted (IB) using anti-V5 and anti-HA antibodies, as in ( a ). ( c ) HeLa cells transfected with empty vector (EV) or V5/His6-tagged SMSr were solubilized using detergent in the presence or absence of 10 mM N -ethyl maleimide (NEM) and subjected to Ni 2+ -NTA affinity chromatography. Ni 2+ -NTA eluates were incubated in the presence or absence of 100 mM DTT, and immunoblotted (IB) using an anti-V5 antibody, as in ( a ). ( d ) HeLa cells transfected with HA-tagged SMSr were treated with chemical crosslinker DSP (0–50 μM, 15 min, RT), solubilized by detergent in the presence of 10 mM NEM and subjected to immunoprecipitation analysis using an anti-HA antibody. Immunoprecipitates were immunoblotted using an anti-HA antibody. ( e ) Yeast cells transfected with empty vector (EV) or V5/His6-tagged SMSr were treated with DSP as in ( d ), lysed and subjected to immunoblot analysis using an anti-V5 antibody. ( f ) HeLa cells transfected with empty vector (EV), V5/His6-tagged SMSr or SMSr∆SAM were incubated in the presence or absence of DSP (50 μM, 15 min, RT), solubilized with detergent in the presence of 10 mM NEM and subjected to Ni 2+ -NTA affinity chromatography. Ni 2+ -NTA eluates were immunoblotted using an anti-V5 antibody. Uncropped images of blots are provided in Supplementary Fig. S1 .

    Article Snippet: Reagents N -ethyl maleimide (NEM), dithiothreitol (DTT) and myriocin were from Sigma-Aldrich, 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn -glycero-3-phosphoethanol-amine (DOPE) and 1-palmitoyl-2-{6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl}-sn -glycero-3-phosphoethanolamine (NBD-PE) from Avanti Polar Lipids, and dithiobis(succinimidyl-propionate) (DSP), mouse monoclonal anti-HA agarose beads, C6 -NBD-ceramide and N3 -Alexa-Fluor647 from Thermo Fischer Scientific.

    Techniques: Transfection, Immunoprecipitation, Plasmid Preparation, Affinity Chromatography, Incubation