Journal: International Journal of Molecular Sciences
Article Title: Cellular Pharmacology of Palladinum(III) Hematoporphyrin IX Complexes: Solution Stability, Antineoplastic and Apoptogenic Activity, DNA Binding, and Processing of DNA-Adducts
Figure Lengend Snippet: Metallation of a 40-base DNA fragment following treatment with cisplatin (molar ratio 1:50) and Pd1 (molar ratios 1:100 and 1:200). The level of metal binding following interactions between the tested complexes and the DNA probe was analyzed after BamH1 treatment, electrophoretic analysis in 5% native polyacrilamide gel, and ethidium bromide staining. On the picture: C , unmodified DNA; CB , BamH1-digested DNA; cis , cisplatin-modified DNA; cisB , cisplatin-modified DNA (1:50) BamH1-digested; Pd , Pd1 -modified DNA; PdIB , Pd1 -modified DNA (1:100) BamH1-digested, PdIIB , Pd1 -modified DNA (1:200) BamH1-digested.
Article Snippet: Agarose, ethanol, DMSO, formic acid, 2-propanol, methanol, EDTA, ethidium bromide, sodium chloride, Tris hydrochloride, Triton® X-100, l -glutamine were purchased from AppliChem GmbH, Darmstadt, Germany.
Techniques: Binding Assay, Staining, Modification