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  • 99
    Thermo Fisher pierce ltq esi positive ion calibration solution
    Pierce Ltq Esi Positive Ion Calibration Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pierce ltq esi positive ion calibration solution/product/Thermo Fisher
    Average 99 stars, based on 38 article reviews
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    94
    Millipore proteomass esi calibration kit mscal5
    Proteomass Esi Calibration Kit Mscal5, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/proteomass esi calibration kit mscal5/product/Millipore
    Average 94 stars, based on 42 article reviews
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    90
    Waters Corporation electrospray ionization mode
    Targeted metabolomics profiles in the GBS patients, the multiple sclerosis (MS) patients, and the healthy controls (control). Extracted plasma from GBS patients (n = 38), MS patients (n = 22) and healthy controls (n = 40) were analyzed by LC-MS/MS and FIA-MS/MS in <t>electrospray</t> positive and negative ion mode. ( A ) The orthogonal partial least squares discriminant analysis (OPLS-DA) demonstrates a clear separation of metabolites between GBS, MS, and control cases (R2 = 0.503, Q2 = 0.338). ( B ) A Venn diagram was used to visualize the number of extremely different metabolites (p
    Electrospray Ionization Mode, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 90/100, based on 187 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/electrospray ionization mode/product/Waters Corporation
    Average 90 stars, based on 187 article reviews
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    91
    Agilent technologies esi mode
    Detection of phosphorylated sphingosine analogs. (A) LC-HRMS verification of the identity of the clickable sphingosine analogs used and detection of a phosphorylation product of ω-N 3 -sphingosine catalyzed by SphK1 in vitro . Lipid extracts from incubation of 1-N 3 -sphingosine or ω-N 3 -sphingosine with SphK1 and ATP were chromatographically separated by HPLC and analyzed with a quadrupole-time-of-flight mass spectrometer (QTOF MS) operating in the positive <t>electrospray</t> ionization mode <t>(ESI+).</t> Chemical structures and mass spectra of ionized ω-N 3 -sphingosine (left), 1-N 3 -sphingosine (middle), and phosphorylated ω-N 3 -S1P (right) are shown. No phosphorylation product of 1-N 3 -sphingosine could be detected. The retention time (t R ) of each substance analyzed is given as inset (top left) in the respective mass spectrum. (B) Detection of ω-N 3 -sphingosine 1-phosphate in Chang cells by LC-MS/MS. Chang cells were incubated with either 1-N 3 -sphingosine or ω-N 3 -sphingosine (10 μM) for 17 h. Lipid extracts were analyzed by selected reaction monitoring (SRM) after positive electrospray ionization (ESI+). As expected, no phosphorylation product could be detected for 1-N 3 -sphingosine (not shown). On the other hand, both ω-N 3 -sphingosine (upper chromatogram) and ω-N 3 -S1P (lower chromatogram) were present in lipid extracts of Chang cells stimulated with ω-N 3 -sphingosine. Assuming similar MS/MS responses for precursor and phosphorylation product, a conversion of about 20% occurred under the given experimental conditions. Peaks are labeled with retention time. Structural formulas and MS/MS fragmentations monitored are given as insets. CID, collision-induced dissociation.
    Esi Mode, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 287 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/esi mode/product/Agilent technologies
    Average 91 stars, based on 287 article reviews
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    99
    Thermo Fisher electrospray ionization mode
    Detection of phosphorylated sphingosine analogs. (A) LC-HRMS verification of the identity of the clickable sphingosine analogs used and detection of a phosphorylation product of ω-N 3 -sphingosine catalyzed by SphK1 in vitro . Lipid extracts from incubation of 1-N 3 -sphingosine or ω-N 3 -sphingosine with SphK1 and ATP were chromatographically separated by HPLC and analyzed with a quadrupole-time-of-flight mass spectrometer (QTOF MS) operating in the positive <t>electrospray</t> ionization mode <t>(ESI+).</t> Chemical structures and mass spectra of ionized ω-N 3 -sphingosine (left), 1-N 3 -sphingosine (middle), and phosphorylated ω-N 3 -S1P (right) are shown. No phosphorylation product of 1-N 3 -sphingosine could be detected. The retention time (t R ) of each substance analyzed is given as inset (top left) in the respective mass spectrum. (B) Detection of ω-N 3 -sphingosine 1-phosphate in Chang cells by LC-MS/MS. Chang cells were incubated with either 1-N 3 -sphingosine or ω-N 3 -sphingosine (10 μM) for 17 h. Lipid extracts were analyzed by selected reaction monitoring (SRM) after positive electrospray ionization (ESI+). As expected, no phosphorylation product could be detected for 1-N 3 -sphingosine (not shown). On the other hand, both ω-N 3 -sphingosine (upper chromatogram) and ω-N 3 -S1P (lower chromatogram) were present in lipid extracts of Chang cells stimulated with ω-N 3 -sphingosine. Assuming similar MS/MS responses for precursor and phosphorylation product, a conversion of about 20% occurred under the given experimental conditions. Peaks are labeled with retention time. Structural formulas and MS/MS fragmentations monitored are given as insets. CID, collision-induced dissociation.
    Electrospray Ionization Mode, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/electrospray ionization mode/product/Thermo Fisher
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    88
    Supelco proteomass ltq ft hybrid esi positive mode cal mix
    Detection of phosphorylated sphingosine analogs. (A) LC-HRMS verification of the identity of the clickable sphingosine analogs used and detection of a phosphorylation product of ω-N 3 -sphingosine catalyzed by SphK1 in vitro . Lipid extracts from incubation of 1-N 3 -sphingosine or ω-N 3 -sphingosine with SphK1 and ATP were chromatographically separated by HPLC and analyzed with a quadrupole-time-of-flight mass spectrometer (QTOF MS) operating in the positive <t>electrospray</t> ionization mode <t>(ESI+).</t> Chemical structures and mass spectra of ionized ω-N 3 -sphingosine (left), 1-N 3 -sphingosine (middle), and phosphorylated ω-N 3 -S1P (right) are shown. No phosphorylation product of 1-N 3 -sphingosine could be detected. The retention time (t R ) of each substance analyzed is given as inset (top left) in the respective mass spectrum. (B) Detection of ω-N 3 -sphingosine 1-phosphate in Chang cells by LC-MS/MS. Chang cells were incubated with either 1-N 3 -sphingosine or ω-N 3 -sphingosine (10 μM) for 17 h. Lipid extracts were analyzed by selected reaction monitoring (SRM) after positive electrospray ionization (ESI+). As expected, no phosphorylation product could be detected for 1-N 3 -sphingosine (not shown). On the other hand, both ω-N 3 -sphingosine (upper chromatogram) and ω-N 3 -S1P (lower chromatogram) were present in lipid extracts of Chang cells stimulated with ω-N 3 -sphingosine. Assuming similar MS/MS responses for precursor and phosphorylation product, a conversion of about 20% occurred under the given experimental conditions. Peaks are labeled with retention time. Structural formulas and MS/MS fragmentations monitored are given as insets. CID, collision-induced dissociation.
    Proteomass Ltq Ft Hybrid Esi Positive Mode Cal Mix, supplied by Supelco, used in various techniques. Bioz Stars score: 88/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Waters Corporation electrospray positive ionization mode
    Detection of phosphorylated sphingosine analogs. (A) LC-HRMS verification of the identity of the clickable sphingosine analogs used and detection of a phosphorylation product of ω-N 3 -sphingosine catalyzed by SphK1 in vitro . Lipid extracts from incubation of 1-N 3 -sphingosine or ω-N 3 -sphingosine with SphK1 and ATP were chromatographically separated by HPLC and analyzed with a quadrupole-time-of-flight mass spectrometer (QTOF MS) operating in the positive <t>electrospray</t> ionization mode <t>(ESI+).</t> Chemical structures and mass spectra of ionized ω-N 3 -sphingosine (left), 1-N 3 -sphingosine (middle), and phosphorylated ω-N 3 -S1P (right) are shown. No phosphorylation product of 1-N 3 -sphingosine could be detected. The retention time (t R ) of each substance analyzed is given as inset (top left) in the respective mass spectrum. (B) Detection of ω-N 3 -sphingosine 1-phosphate in Chang cells by LC-MS/MS. Chang cells were incubated with either 1-N 3 -sphingosine or ω-N 3 -sphingosine (10 μM) for 17 h. Lipid extracts were analyzed by selected reaction monitoring (SRM) after positive electrospray ionization (ESI+). As expected, no phosphorylation product could be detected for 1-N 3 -sphingosine (not shown). On the other hand, both ω-N 3 -sphingosine (upper chromatogram) and ω-N 3 -S1P (lower chromatogram) were present in lipid extracts of Chang cells stimulated with ω-N 3 -sphingosine. Assuming similar MS/MS responses for precursor and phosphorylation product, a conversion of about 20% occurred under the given experimental conditions. Peaks are labeled with retention time. Structural formulas and MS/MS fragmentations monitored are given as insets. CID, collision-induced dissociation.
    Electrospray Positive Ionization Mode, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 88/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/electrospray positive ionization mode/product/Waters Corporation
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    90
    Waters Corporation ion mode electrospray ionization
    Detection of phosphorylated sphingosine analogs. (A) LC-HRMS verification of the identity of the clickable sphingosine analogs used and detection of a phosphorylation product of ω-N 3 -sphingosine catalyzed by SphK1 in vitro . Lipid extracts from incubation of 1-N 3 -sphingosine or ω-N 3 -sphingosine with SphK1 and ATP were chromatographically separated by HPLC and analyzed with a quadrupole-time-of-flight mass spectrometer (QTOF MS) operating in the positive <t>electrospray</t> ionization mode <t>(ESI+).</t> Chemical structures and mass spectra of ionized ω-N 3 -sphingosine (left), 1-N 3 -sphingosine (middle), and phosphorylated ω-N 3 -S1P (right) are shown. No phosphorylation product of 1-N 3 -sphingosine could be detected. The retention time (t R ) of each substance analyzed is given as inset (top left) in the respective mass spectrum. (B) Detection of ω-N 3 -sphingosine 1-phosphate in Chang cells by LC-MS/MS. Chang cells were incubated with either 1-N 3 -sphingosine or ω-N 3 -sphingosine (10 μM) for 17 h. Lipid extracts were analyzed by selected reaction monitoring (SRM) after positive electrospray ionization (ESI+). As expected, no phosphorylation product could be detected for 1-N 3 -sphingosine (not shown). On the other hand, both ω-N 3 -sphingosine (upper chromatogram) and ω-N 3 -S1P (lower chromatogram) were present in lipid extracts of Chang cells stimulated with ω-N 3 -sphingosine. Assuming similar MS/MS responses for precursor and phosphorylation product, a conversion of about 20% occurred under the given experimental conditions. Peaks are labeled with retention time. Structural formulas and MS/MS fragmentations monitored are given as insets. CID, collision-induced dissociation.
    Ion Mode Electrospray Ionization, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 90/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ion mode electrospray ionization/product/Waters Corporation
    Average 90 stars, based on 17 article reviews
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    ion mode electrospray ionization - by Bioz Stars, 2021-01
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    99
    Thermo Fisher pierce negative ion calibration solution
    Detection of phosphorylated sphingosine analogs. (A) LC-HRMS verification of the identity of the clickable sphingosine analogs used and detection of a phosphorylation product of ω-N 3 -sphingosine catalyzed by SphK1 in vitro . Lipid extracts from incubation of 1-N 3 -sphingosine or ω-N 3 -sphingosine with SphK1 and ATP were chromatographically separated by HPLC and analyzed with a quadrupole-time-of-flight mass spectrometer (QTOF MS) operating in the positive <t>electrospray</t> ionization mode <t>(ESI+).</t> Chemical structures and mass spectra of ionized ω-N 3 -sphingosine (left), 1-N 3 -sphingosine (middle), and phosphorylated ω-N 3 -S1P (right) are shown. No phosphorylation product of 1-N 3 -sphingosine could be detected. The retention time (t R ) of each substance analyzed is given as inset (top left) in the respective mass spectrum. (B) Detection of ω-N 3 -sphingosine 1-phosphate in Chang cells by LC-MS/MS. Chang cells were incubated with either 1-N 3 -sphingosine or ω-N 3 -sphingosine (10 μM) for 17 h. Lipid extracts were analyzed by selected reaction monitoring (SRM) after positive electrospray ionization (ESI+). As expected, no phosphorylation product could be detected for 1-N 3 -sphingosine (not shown). On the other hand, both ω-N 3 -sphingosine (upper chromatogram) and ω-N 3 -S1P (lower chromatogram) were present in lipid extracts of Chang cells stimulated with ω-N 3 -sphingosine. Assuming similar MS/MS responses for precursor and phosphorylation product, a conversion of about 20% occurred under the given experimental conditions. Peaks are labeled with retention time. Structural formulas and MS/MS fragmentations monitored are given as insets. CID, collision-induced dissociation.
    Pierce Negative Ion Calibration Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pierce negative ion calibration solution/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
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    99
    Agilent technologies apci esi ionization mode
    Detection of phosphorylated sphingosine analogs. (A) LC-HRMS verification of the identity of the clickable sphingosine analogs used and detection of a phosphorylation product of ω-N 3 -sphingosine catalyzed by SphK1 in vitro . Lipid extracts from incubation of 1-N 3 -sphingosine or ω-N 3 -sphingosine with SphK1 and ATP were chromatographically separated by HPLC and analyzed with a quadrupole-time-of-flight mass spectrometer (QTOF MS) operating in the positive <t>electrospray</t> ionization mode <t>(ESI+).</t> Chemical structures and mass spectra of ionized ω-N 3 -sphingosine (left), 1-N 3 -sphingosine (middle), and phosphorylated ω-N 3 -S1P (right) are shown. No phosphorylation product of 1-N 3 -sphingosine could be detected. The retention time (t R ) of each substance analyzed is given as inset (top left) in the respective mass spectrum. (B) Detection of ω-N 3 -sphingosine 1-phosphate in Chang cells by LC-MS/MS. Chang cells were incubated with either 1-N 3 -sphingosine or ω-N 3 -sphingosine (10 μM) for 17 h. Lipid extracts were analyzed by selected reaction monitoring (SRM) after positive electrospray ionization (ESI+). As expected, no phosphorylation product could be detected for 1-N 3 -sphingosine (not shown). On the other hand, both ω-N 3 -sphingosine (upper chromatogram) and ω-N 3 -S1P (lower chromatogram) were present in lipid extracts of Chang cells stimulated with ω-N 3 -sphingosine. Assuming similar MS/MS responses for precursor and phosphorylation product, a conversion of about 20% occurred under the given experimental conditions. Peaks are labeled with retention time. Structural formulas and MS/MS fragmentations monitored are given as insets. CID, collision-induced dissociation.
    Apci Esi Ionization Mode, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apci esi ionization mode/product/Agilent technologies
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    90
    Vion Pharma esi mode
    Detection of phosphorylated sphingosine analogs. (A) LC-HRMS verification of the identity of the clickable sphingosine analogs used and detection of a phosphorylation product of ω-N 3 -sphingosine catalyzed by SphK1 in vitro . Lipid extracts from incubation of 1-N 3 -sphingosine or ω-N 3 -sphingosine with SphK1 and ATP were chromatographically separated by HPLC and analyzed with a quadrupole-time-of-flight mass spectrometer (QTOF MS) operating in the positive <t>electrospray</t> ionization mode <t>(ESI+).</t> Chemical structures and mass spectra of ionized ω-N 3 -sphingosine (left), 1-N 3 -sphingosine (middle), and phosphorylated ω-N 3 -S1P (right) are shown. No phosphorylation product of 1-N 3 -sphingosine could be detected. The retention time (t R ) of each substance analyzed is given as inset (top left) in the respective mass spectrum. (B) Detection of ω-N 3 -sphingosine 1-phosphate in Chang cells by LC-MS/MS. Chang cells were incubated with either 1-N 3 -sphingosine or ω-N 3 -sphingosine (10 μM) for 17 h. Lipid extracts were analyzed by selected reaction monitoring (SRM) after positive electrospray ionization (ESI+). As expected, no phosphorylation product could be detected for 1-N 3 -sphingosine (not shown). On the other hand, both ω-N 3 -sphingosine (upper chromatogram) and ω-N 3 -S1P (lower chromatogram) were present in lipid extracts of Chang cells stimulated with ω-N 3 -sphingosine. Assuming similar MS/MS responses for precursor and phosphorylation product, a conversion of about 20% occurred under the given experimental conditions. Peaks are labeled with retention time. Structural formulas and MS/MS fragmentations monitored are given as insets. CID, collision-induced dissociation.
    Esi Mode, supplied by Vion Pharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/esi mode/product/Vion Pharma
    Average 90 stars, based on 1 article reviews
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    91
    Agilent technologies esi mass spectrum mode
    Detection of phosphorylated sphingosine analogs. (A) LC-HRMS verification of the identity of the clickable sphingosine analogs used and detection of a phosphorylation product of ω-N 3 -sphingosine catalyzed by SphK1 in vitro . Lipid extracts from incubation of 1-N 3 -sphingosine or ω-N 3 -sphingosine with SphK1 and ATP were chromatographically separated by HPLC and analyzed with a quadrupole-time-of-flight mass spectrometer (QTOF MS) operating in the positive <t>electrospray</t> ionization mode <t>(ESI+).</t> Chemical structures and mass spectra of ionized ω-N 3 -sphingosine (left), 1-N 3 -sphingosine (middle), and phosphorylated ω-N 3 -S1P (right) are shown. No phosphorylation product of 1-N 3 -sphingosine could be detected. The retention time (t R ) of each substance analyzed is given as inset (top left) in the respective mass spectrum. (B) Detection of ω-N 3 -sphingosine 1-phosphate in Chang cells by LC-MS/MS. Chang cells were incubated with either 1-N 3 -sphingosine or ω-N 3 -sphingosine (10 μM) for 17 h. Lipid extracts were analyzed by selected reaction monitoring (SRM) after positive electrospray ionization (ESI+). As expected, no phosphorylation product could be detected for 1-N 3 -sphingosine (not shown). On the other hand, both ω-N 3 -sphingosine (upper chromatogram) and ω-N 3 -S1P (lower chromatogram) were present in lipid extracts of Chang cells stimulated with ω-N 3 -sphingosine. Assuming similar MS/MS responses for precursor and phosphorylation product, a conversion of about 20% occurred under the given experimental conditions. Peaks are labeled with retention time. Structural formulas and MS/MS fragmentations monitored are given as insets. CID, collision-induced dissociation.
    Esi Mass Spectrum Mode, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/esi mass spectrum mode/product/Agilent technologies
    Average 91 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    esi mass spectrum mode - by Bioz Stars, 2021-01
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    Image Search Results


    Targeted metabolomics profiles in the GBS patients, the multiple sclerosis (MS) patients, and the healthy controls (control). Extracted plasma from GBS patients (n = 38), MS patients (n = 22) and healthy controls (n = 40) were analyzed by LC-MS/MS and FIA-MS/MS in electrospray positive and negative ion mode. ( A ) The orthogonal partial least squares discriminant analysis (OPLS-DA) demonstrates a clear separation of metabolites between GBS, MS, and control cases (R2 = 0.503, Q2 = 0.338). ( B ) A Venn diagram was used to visualize the number of extremely different metabolites (p

    Journal: Scientific Reports

    Article Title: Disturbance of Plasma Lipid Metabolic Profile in Guillain-Barre Syndrome

    doi: 10.1038/s41598-017-08338-7

    Figure Lengend Snippet: Targeted metabolomics profiles in the GBS patients, the multiple sclerosis (MS) patients, and the healthy controls (control). Extracted plasma from GBS patients (n = 38), MS patients (n = 22) and healthy controls (n = 40) were analyzed by LC-MS/MS and FIA-MS/MS in electrospray positive and negative ion mode. ( A ) The orthogonal partial least squares discriminant analysis (OPLS-DA) demonstrates a clear separation of metabolites between GBS, MS, and control cases (R2 = 0.503, Q2 = 0.338). ( B ) A Venn diagram was used to visualize the number of extremely different metabolites (p

    Article Snippet: The analysis was performed in positive electrospray ionization mode with multiple reaction monitoring (Waters, Milford, USA).

    Techniques: Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy

    Effect of LTA4H variants on gene expression and ex vivo LTB 4 production. a LTA4H mRNA levels in monocytes are not significantly different between carriers and non-carriers of the HapK or rs2540477 variants. Real-time PCR was carried out in triplicate and expression levels were normalized to GUSB as an endogenous control. b Monocytes isolated from carriers of HapK or rs2540477 produce significantly higher levels of LTB 4 than non-carriers. Monocytes were isolated from healthy subjects and stimulated with the calcium ionophore A23187 for 60 min. LTB 4 was measured in the supernatant by negative mode electrospray ionization tandem mass spectrometry. Data are shown as mean ± SE and the number of samples analyzed for each genotype is given in parentheses

    Journal: Human Genetics

    Article Title: Genetic contribution of the leukotriene pathway to coronary artery disease

    doi: 10.1007/s00439-011-0963-3

    Figure Lengend Snippet: Effect of LTA4H variants on gene expression and ex vivo LTB 4 production. a LTA4H mRNA levels in monocytes are not significantly different between carriers and non-carriers of the HapK or rs2540477 variants. Real-time PCR was carried out in triplicate and expression levels were normalized to GUSB as an endogenous control. b Monocytes isolated from carriers of HapK or rs2540477 produce significantly higher levels of LTB 4 than non-carriers. Monocytes were isolated from healthy subjects and stimulated with the calcium ionophore A23187 for 60 min. LTB 4 was measured in the supernatant by negative mode electrospray ionization tandem mass spectrometry. Data are shown as mean ± SE and the number of samples analyzed for each genotype is given in parentheses

    Article Snippet: Oxylipid analytes, including LTB4 , were chromatographically separated on an ultra-performance liquid chromatography system equipped with a 2.1 × 150 mm Acquity BEHC18 reversed phase column and quantified by negative mode electrospray ionization on a Quattro Micro tandem mass spectrometer (Waters, Inc.).

    Techniques: Expressing, Ex Vivo, Real-time Polymerase Chain Reaction, Isolation, Mass Spectrometry

    Detection of phosphorylated sphingosine analogs. (A) LC-HRMS verification of the identity of the clickable sphingosine analogs used and detection of a phosphorylation product of ω-N 3 -sphingosine catalyzed by SphK1 in vitro . Lipid extracts from incubation of 1-N 3 -sphingosine or ω-N 3 -sphingosine with SphK1 and ATP were chromatographically separated by HPLC and analyzed with a quadrupole-time-of-flight mass spectrometer (QTOF MS) operating in the positive electrospray ionization mode (ESI+). Chemical structures and mass spectra of ionized ω-N 3 -sphingosine (left), 1-N 3 -sphingosine (middle), and phosphorylated ω-N 3 -S1P (right) are shown. No phosphorylation product of 1-N 3 -sphingosine could be detected. The retention time (t R ) of each substance analyzed is given as inset (top left) in the respective mass spectrum. (B) Detection of ω-N 3 -sphingosine 1-phosphate in Chang cells by LC-MS/MS. Chang cells were incubated with either 1-N 3 -sphingosine or ω-N 3 -sphingosine (10 μM) for 17 h. Lipid extracts were analyzed by selected reaction monitoring (SRM) after positive electrospray ionization (ESI+). As expected, no phosphorylation product could be detected for 1-N 3 -sphingosine (not shown). On the other hand, both ω-N 3 -sphingosine (upper chromatogram) and ω-N 3 -S1P (lower chromatogram) were present in lipid extracts of Chang cells stimulated with ω-N 3 -sphingosine. Assuming similar MS/MS responses for precursor and phosphorylation product, a conversion of about 20% occurred under the given experimental conditions. Peaks are labeled with retention time. Structural formulas and MS/MS fragmentations monitored are given as insets. CID, collision-induced dissociation.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: A Role of Sphingosine in the Intracellular Survival of Neisseria gonorrhoeae

    doi: 10.3389/fcimb.2020.00215

    Figure Lengend Snippet: Detection of phosphorylated sphingosine analogs. (A) LC-HRMS verification of the identity of the clickable sphingosine analogs used and detection of a phosphorylation product of ω-N 3 -sphingosine catalyzed by SphK1 in vitro . Lipid extracts from incubation of 1-N 3 -sphingosine or ω-N 3 -sphingosine with SphK1 and ATP were chromatographically separated by HPLC and analyzed with a quadrupole-time-of-flight mass spectrometer (QTOF MS) operating in the positive electrospray ionization mode (ESI+). Chemical structures and mass spectra of ionized ω-N 3 -sphingosine (left), 1-N 3 -sphingosine (middle), and phosphorylated ω-N 3 -S1P (right) are shown. No phosphorylation product of 1-N 3 -sphingosine could be detected. The retention time (t R ) of each substance analyzed is given as inset (top left) in the respective mass spectrum. (B) Detection of ω-N 3 -sphingosine 1-phosphate in Chang cells by LC-MS/MS. Chang cells were incubated with either 1-N 3 -sphingosine or ω-N 3 -sphingosine (10 μM) for 17 h. Lipid extracts were analyzed by selected reaction monitoring (SRM) after positive electrospray ionization (ESI+). As expected, no phosphorylation product could be detected for 1-N 3 -sphingosine (not shown). On the other hand, both ω-N 3 -sphingosine (upper chromatogram) and ω-N 3 -S1P (lower chromatogram) were present in lipid extracts of Chang cells stimulated with ω-N 3 -sphingosine. Assuming similar MS/MS responses for precursor and phosphorylation product, a conversion of about 20% occurred under the given experimental conditions. Peaks are labeled with retention time. Structural formulas and MS/MS fragmentations monitored are given as insets. CID, collision-induced dissociation.

    Article Snippet: Briefly, chromatographic separations were performed with an Agilent 1260 Infinity HPLC coupled to an Agilent 6530 quadrupole-time-of-flight mass spectrometer (QTOF MS) operating in the positive electrospray ionization (ESI+) mode (Agilent Technologies, Waldbronn, Germany).

    Techniques: In Vitro, Incubation, High Performance Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy, Tandem Mass Spectroscopy, Labeling