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  • 89
    Agilent technologies electrospray ionization esi mode
    Detection of phosphorylated sphingosine analogs. (A) LC-HRMS verification of the identity of the clickable sphingosine analogs used and detection of a phosphorylation product of ω-N 3 -sphingosine catalyzed by SphK1 in vitro . Lipid extracts from incubation of 1-N 3 -sphingosine or ω-N 3 -sphingosine with SphK1 and ATP were chromatographically separated by HPLC and analyzed with a quadrupole-time-of-flight mass spectrometer (QTOF MS) operating in the positive <t>electrospray</t> ionization mode <t>(ESI+).</t> Chemical structures and mass spectra of ionized ω-N 3 -sphingosine (left), 1-N 3 -sphingosine (middle), and phosphorylated ω-N 3 -S1P (right) are shown. No phosphorylation product of 1-N 3 -sphingosine could be detected. The retention time (t R ) of each substance analyzed is given as inset (top left) in the respective mass spectrum. (B) Detection of ω-N 3 -sphingosine 1-phosphate in Chang cells by LC-MS/MS. Chang cells were incubated with either 1-N 3 -sphingosine or ω-N 3 -sphingosine (10 μM) for 17 h. Lipid extracts were analyzed by selected reaction monitoring (SRM) after positive electrospray ionization (ESI+). As expected, no phosphorylation product could be detected for 1-N 3 -sphingosine (not shown). On the other hand, both ω-N 3 -sphingosine (upper chromatogram) and ω-N 3 -S1P (lower chromatogram) were present in lipid extracts of Chang cells stimulated with ω-N 3 -sphingosine. Assuming similar MS/MS responses for precursor and phosphorylation product, a conversion of about 20% occurred under the given experimental conditions. Peaks are labeled with retention time. Structural formulas and MS/MS fragmentations monitored are given as insets. CID, collision-induced dissociation.
    Electrospray Ionization Esi Mode, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Waters Corporation electrospray ionization mode esi
    Targeted metabolomics profiles in the GBS patients, the multiple sclerosis (MS) patients, and the healthy controls (control). Extracted plasma from GBS patients (n = 38), MS patients (n = 22) and healthy controls (n = 40) were analyzed by LC-MS/MS and FIA-MS/MS in <t>electrospray</t> positive and negative ion mode. ( A ) The orthogonal partial least squares discriminant analysis (OPLS-DA) demonstrates a clear separation of metabolites between GBS, MS, and control cases (R2 = 0.503, Q2 = 0.338). ( B ) A Venn diagram was used to visualize the number of extremely different metabolites (p
    Electrospray Ionization Mode Esi, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 91/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    SCIEX electrospray ionization esi mode
    Targeted metabolomics profiles in the GBS patients, the multiple sclerosis (MS) patients, and the healthy controls (control). Extracted plasma from GBS patients (n = 38), MS patients (n = 22) and healthy controls (n = 40) were analyzed by LC-MS/MS and FIA-MS/MS in <t>electrospray</t> positive and negative ion mode. ( A ) The orthogonal partial least squares discriminant analysis (OPLS-DA) demonstrates a clear separation of metabolites between GBS, MS, and control cases (R2 = 0.503, Q2 = 0.338). ( B ) A Venn diagram was used to visualize the number of extremely different metabolites (p
    Electrospray Ionization Esi Mode, supplied by SCIEX, used in various techniques. Bioz Stars score: 93/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher mode electrospray ionization esi technique
    <t>Electrospray</t> ionization <t>(ESI)</t> mass spectra of a compound 1 and b compound 2
    Mode Electrospray Ionization Esi Technique, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Bruker Corporation esi electrospray ionization mode
    <t>Electrospray</t> ionization <t>(ESI)</t> mass spectra of a compound 1 and b compound 2
    Esi Electrospray Ionization Mode, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Shimadzu Corporation electrospray ionization esi mode
    Determination of reduction potentials of cSelS and cSelS U188C in GSH/GSSG redox buffers. The ratio of reduced and oxidized protein was determined by <t>electrospray</t> ionization mass spectroscopy. (A) A representative electrospray ionization mass spectrum of cSelS following incubation in a redox buffer poised at −232 mV and subsequent alkylation. The panel shows the charge state distribution of multiply charged ions [M+18H] 18+ to [M+22H] 22+ from m/z 680 to 870. (B) Deconvoluted spectrum. Molecular weights: alkylated cSelS 188Δ 15149 Da, oxidized cSelS 15300 Da and alkylated cSelS 15414 Da. (C) A representative electrospray ionization mass spectrum of cSelS U188C following incubation in a redox buffer poised at −209 mV and subsequent alkylation. The panel shows the charge state distribution of multiply charged ions [M+18H] 18+ to [M+22H] 22+ from m/z 685 to 870. (D) Deconvoluted spectrum. Molecular weights: oxidized cSelS U188C 15253 Da and alkylated cSelS U188C 15365 Da. Panels E and F display the resulting titration curves for (E) cSelS, reduction potential −234±1 mV; and (F) cSelS U188C, reduction potential −211±1 mV. The fraction of reduced protein is plotted against the buffer redox potential poised by the ratio of GSH to GSSG. The error bars represent the range of measurements (that is, highest and lowest values) among three repetitions, using two independent protein preparations.
    Electrospray Ionization Esi Mode, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 90/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Detection of phosphorylated sphingosine analogs. (A) LC-HRMS verification of the identity of the clickable sphingosine analogs used and detection of a phosphorylation product of ω-N 3 -sphingosine catalyzed by SphK1 in vitro . Lipid extracts from incubation of 1-N 3 -sphingosine or ω-N 3 -sphingosine with SphK1 and ATP were chromatographically separated by HPLC and analyzed with a quadrupole-time-of-flight mass spectrometer (QTOF MS) operating in the positive electrospray ionization mode (ESI+). Chemical structures and mass spectra of ionized ω-N 3 -sphingosine (left), 1-N 3 -sphingosine (middle), and phosphorylated ω-N 3 -S1P (right) are shown. No phosphorylation product of 1-N 3 -sphingosine could be detected. The retention time (t R ) of each substance analyzed is given as inset (top left) in the respective mass spectrum. (B) Detection of ω-N 3 -sphingosine 1-phosphate in Chang cells by LC-MS/MS. Chang cells were incubated with either 1-N 3 -sphingosine or ω-N 3 -sphingosine (10 μM) for 17 h. Lipid extracts were analyzed by selected reaction monitoring (SRM) after positive electrospray ionization (ESI+). As expected, no phosphorylation product could be detected for 1-N 3 -sphingosine (not shown). On the other hand, both ω-N 3 -sphingosine (upper chromatogram) and ω-N 3 -S1P (lower chromatogram) were present in lipid extracts of Chang cells stimulated with ω-N 3 -sphingosine. Assuming similar MS/MS responses for precursor and phosphorylation product, a conversion of about 20% occurred under the given experimental conditions. Peaks are labeled with retention time. Structural formulas and MS/MS fragmentations monitored are given as insets. CID, collision-induced dissociation.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: A Role of Sphingosine in the Intracellular Survival of Neisseria gonorrhoeae

    doi: 10.3389/fcimb.2020.00215

    Figure Lengend Snippet: Detection of phosphorylated sphingosine analogs. (A) LC-HRMS verification of the identity of the clickable sphingosine analogs used and detection of a phosphorylation product of ω-N 3 -sphingosine catalyzed by SphK1 in vitro . Lipid extracts from incubation of 1-N 3 -sphingosine or ω-N 3 -sphingosine with SphK1 and ATP were chromatographically separated by HPLC and analyzed with a quadrupole-time-of-flight mass spectrometer (QTOF MS) operating in the positive electrospray ionization mode (ESI+). Chemical structures and mass spectra of ionized ω-N 3 -sphingosine (left), 1-N 3 -sphingosine (middle), and phosphorylated ω-N 3 -S1P (right) are shown. No phosphorylation product of 1-N 3 -sphingosine could be detected. The retention time (t R ) of each substance analyzed is given as inset (top left) in the respective mass spectrum. (B) Detection of ω-N 3 -sphingosine 1-phosphate in Chang cells by LC-MS/MS. Chang cells were incubated with either 1-N 3 -sphingosine or ω-N 3 -sphingosine (10 μM) for 17 h. Lipid extracts were analyzed by selected reaction monitoring (SRM) after positive electrospray ionization (ESI+). As expected, no phosphorylation product could be detected for 1-N 3 -sphingosine (not shown). On the other hand, both ω-N 3 -sphingosine (upper chromatogram) and ω-N 3 -S1P (lower chromatogram) were present in lipid extracts of Chang cells stimulated with ω-N 3 -sphingosine. Assuming similar MS/MS responses for precursor and phosphorylation product, a conversion of about 20% occurred under the given experimental conditions. Peaks are labeled with retention time. Structural formulas and MS/MS fragmentations monitored are given as insets. CID, collision-induced dissociation.

    Article Snippet: Briefly, chromatographic separations were performed with an Agilent 1260 Infinity HPLC coupled to an Agilent 6530 quadrupole-time-of-flight mass spectrometer (QTOF MS) operating in the positive electrospray ionization (ESI+) mode (Agilent Technologies, Waldbronn, Germany).

    Techniques: In Vitro, Incubation, High Performance Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy, Tandem Mass Spectroscopy, Labeling

    Targeted metabolomics profiles in the GBS patients, the multiple sclerosis (MS) patients, and the healthy controls (control). Extracted plasma from GBS patients (n = 38), MS patients (n = 22) and healthy controls (n = 40) were analyzed by LC-MS/MS and FIA-MS/MS in electrospray positive and negative ion mode. ( A ) The orthogonal partial least squares discriminant analysis (OPLS-DA) demonstrates a clear separation of metabolites between GBS, MS, and control cases (R2 = 0.503, Q2 = 0.338). ( B ) A Venn diagram was used to visualize the number of extremely different metabolites (p

    Journal: Scientific Reports

    Article Title: Disturbance of Plasma Lipid Metabolic Profile in Guillain-Barre Syndrome

    doi: 10.1038/s41598-017-08338-7

    Figure Lengend Snippet: Targeted metabolomics profiles in the GBS patients, the multiple sclerosis (MS) patients, and the healthy controls (control). Extracted plasma from GBS patients (n = 38), MS patients (n = 22) and healthy controls (n = 40) were analyzed by LC-MS/MS and FIA-MS/MS in electrospray positive and negative ion mode. ( A ) The orthogonal partial least squares discriminant analysis (OPLS-DA) demonstrates a clear separation of metabolites between GBS, MS, and control cases (R2 = 0.503, Q2 = 0.338). ( B ) A Venn diagram was used to visualize the number of extremely different metabolites (p

    Article Snippet: The analysis was performed in positive electrospray ionization mode with multiple reaction monitoring (Waters, Milford, USA).

    Techniques: Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy

    Electrospray ionization (ESI) mass spectra of a compound 1 and b compound 2

    Journal: Forensic Toxicology

    Article Title: Spectroscopic characterization and crystal structures of two cathinone derivatives: N-ethyl-2-amino-1-phenylpropan-1-one (ethcathinone) hydrochloride and N-ethyl-2-amino-1-(4-chlorophenyl)propan-1-one (4-CEC) hydrochloride

    doi: 10.1007/s11419-016-0345-6

    Figure Lengend Snippet: Electrospray ionization (ESI) mass spectra of a compound 1 and b compound 2

    Article Snippet: Liquid chromatography–mass spectrometry (LC–MS) analysis of samples was performed on a Thermo Scientific TSQ Quantum Access Max LC–MS operating in positive electrospray ionization (ESI) mode (Thermo Scientific, Waltham, MA, USA).

    Techniques:

    Determination of reduction potentials of cSelS and cSelS U188C in GSH/GSSG redox buffers. The ratio of reduced and oxidized protein was determined by electrospray ionization mass spectroscopy. (A) A representative electrospray ionization mass spectrum of cSelS following incubation in a redox buffer poised at −232 mV and subsequent alkylation. The panel shows the charge state distribution of multiply charged ions [M+18H] 18+ to [M+22H] 22+ from m/z 680 to 870. (B) Deconvoluted spectrum. Molecular weights: alkylated cSelS 188Δ 15149 Da, oxidized cSelS 15300 Da and alkylated cSelS 15414 Da. (C) A representative electrospray ionization mass spectrum of cSelS U188C following incubation in a redox buffer poised at −209 mV and subsequent alkylation. The panel shows the charge state distribution of multiply charged ions [M+18H] 18+ to [M+22H] 22+ from m/z 685 to 870. (D) Deconvoluted spectrum. Molecular weights: oxidized cSelS U188C 15253 Da and alkylated cSelS U188C 15365 Da. Panels E and F display the resulting titration curves for (E) cSelS, reduction potential −234±1 mV; and (F) cSelS U188C, reduction potential −211±1 mV. The fraction of reduced protein is plotted against the buffer redox potential poised by the ratio of GSH to GSSG. The error bars represent the range of measurements (that is, highest and lowest values) among three repetitions, using two independent protein preparations.

    Journal: Biochemistry

    Article Title: The intrinsically disordered membrane protein selenoprotein S is a reductase in vitro

    doi: 10.1021/bi4001358

    Figure Lengend Snippet: Determination of reduction potentials of cSelS and cSelS U188C in GSH/GSSG redox buffers. The ratio of reduced and oxidized protein was determined by electrospray ionization mass spectroscopy. (A) A representative electrospray ionization mass spectrum of cSelS following incubation in a redox buffer poised at −232 mV and subsequent alkylation. The panel shows the charge state distribution of multiply charged ions [M+18H] 18+ to [M+22H] 22+ from m/z 680 to 870. (B) Deconvoluted spectrum. Molecular weights: alkylated cSelS 188Δ 15149 Da, oxidized cSelS 15300 Da and alkylated cSelS 15414 Da. (C) A representative electrospray ionization mass spectrum of cSelS U188C following incubation in a redox buffer poised at −209 mV and subsequent alkylation. The panel shows the charge state distribution of multiply charged ions [M+18H] 18+ to [M+22H] 22+ from m/z 685 to 870. (D) Deconvoluted spectrum. Molecular weights: oxidized cSelS U188C 15253 Da and alkylated cSelS U188C 15365 Da. Panels E and F display the resulting titration curves for (E) cSelS, reduction potential −234±1 mV; and (F) cSelS U188C, reduction potential −211±1 mV. The fraction of reduced protein is plotted against the buffer redox potential poised by the ratio of GSH to GSSG. The error bars represent the range of measurements (that is, highest and lowest values) among three repetitions, using two independent protein preparations.

    Article Snippet: Mass spectra were obtained using a QTOF Ultima (Waters, MA), operating under positive electrospray ionization (+ESI) mode, connected to an LC-20AD (Shimadzu, Kyoto, Japan).

    Techniques: Mass Spectrometry, Incubation, Titration